AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034500.t1 MTR_0015s0140 98.966 387 4 0 1 387 1 387 0.0 795
MS.gene034500.t1 MTR_0015s0140 96.864 287 9 0 101 387 47 333 0.0 576
MS.gene034500.t1 MTR_7g081020 53.887 373 155 5 9 370 4 370 9.64e-146 419
MS.gene034500.t1 MTR_1g112840 53.403 382 159 5 1 370 1 375 5.14e-145 417
MS.gene034500.t1 MTR_1g116260 53.619 373 152 5 7 370 8 368 2.45e-139 402
MS.gene034500.t1 MTR_2g033910 50.532 376 179 4 1 373 1 372 5.33e-138 398
MS.gene034500.t1 MTR_8g074930 53.043 345 151 3 37 370 29 373 2.43e-133 387
MS.gene034500.t1 MTR_1g106855 55.590 322 140 1 36 354 18 339 1.59e-129 377
MS.gene034500.t1 MTR_1g067210 53.644 343 149 3 38 370 27 369 1.11e-127 372
MS.gene034500.t1 MTR_3g032660 49.109 393 179 8 1 386 1 379 2.02e-123 363
MS.gene034500.t1 MTR_3g032700 48.855 393 180 8 1 386 1 379 9.04e-123 362
MS.gene034500.t1 MTR_7g100240 54.878 328 139 3 38 356 22 349 4.72e-121 355
MS.gene034500.t1 MTR_7g090550 52.459 366 146 10 1 353 1 351 1.16e-119 354
MS.gene034500.t1 MTR_7g081020 53.922 306 126 4 9 305 4 303 1.54e-116 342
MS.gene034500.t1 MTR_7g090530 49.485 388 174 8 1 380 1 374 1.77e-116 346
MS.gene034500.t1 MTR_1g106855 53.455 275 125 1 36 307 18 292 5.38e-102 304
MS.gene034500.t1 MTR_8g074930 53.640 261 118 2 113 370 1 261 1.19e-100 300
MS.gene034500.t1 MTR_3g032590 50.694 288 134 5 105 386 100 385 3.18e-92 284
MS.gene034500.t1 MTR_4g113480 41.176 323 174 7 38 353 34 347 3.51e-70 230
MS.gene034500.t1 MTR_4g113345 35.920 348 182 11 41 380 22 336 7.79e-56 187
MS.gene034500.t1 MTR_7g090540 47.964 221 92 6 165 380 5 207 3.20e-50 170
MS.gene034500.t1 MTR_4g113210 37.838 259 134 7 38 286 16 257 9.46e-50 168
MS.gene034500.t1 MTR_7g060770 31.757 296 172 10 78 353 262 547 1.63e-38 145
MS.gene034500.t1 MTR_4g076560 30.435 299 185 8 78 361 201 491 2.21e-37 141
MS.gene034500.t1 MTR_4g119830 31.698 265 161 6 101 352 230 487 6.40e-34 132
MS.gene034500.t1 MTR_4g098650 28.247 308 155 8 111 358 472 773 2.99e-27 114
MS.gene034500.t1 MTR_4g063905 27.562 283 160 8 118 361 428 704 5.34e-26 110
MS.gene034500.t1 MTR_3g464650 28.777 278 141 8 75 350 90 312 2.38e-25 105
MS.gene034500.t1 MTR_3g464700 30.667 225 115 5 75 298 114 298 4.03e-25 104
MS.gene034500.t1 MTR_5g071550 29.496 278 139 9 79 354 154 376 2.74e-24 103
MS.gene034500.t1 MTR_3g091060 26.269 335 172 11 31 355 74 343 1.40e-22 98.2
MS.gene034500.t1 MTR_1g085530 30.899 178 114 3 180 353 712 884 4.31e-21 95.9
MS.gene034500.t1 MTR_5g071550 30.876 217 107 7 79 293 154 329 1.13e-19 89.7
MS.gene034500.t1 MTR_7g093240 32.667 150 64 4 158 304 4 119 2.28e-19 84.0
MS.gene034500.t1 MTR_3g105730 27.984 243 136 6 79 320 43 247 3.61e-18 84.3
MS.gene034500.t1 MTR_1g014640 28.676 272 136 9 79 350 108 321 4.25e-18 85.1
MS.gene034500.t1 MTR_1g013400 28.676 272 136 9 79 350 108 321 4.25e-18 85.1
MS.gene034500.t1 MTR_3g105730 27.984 243 136 6 79 320 65 269 4.91e-18 84.3
MS.gene034500.t1 MTR_5g024340 28.235 255 136 8 70 316 115 330 5.20e-18 85.1
MS.gene034500.t1 MTR_1g115570 28.571 224 116 7 79 301 126 306 1.67e-17 83.2
MS.gene034500.t1 MTR_1g086350 26.923 260 129 9 77 316 100 318 1.93e-17 83.6
MS.gene034500.t1 MTR_1g086350 26.923 260 129 9 77 316 116 334 1.97e-17 83.6
MS.gene034500.t1 MTR_4g094542 27.419 248 132 9 73 315 144 348 9.39e-17 81.3
MS.gene034500.t1 MTR_4g125810 28.405 257 130 10 56 298 229 445 3.45e-16 80.5
MS.gene034500.t1 MTR_4g037470 26.667 315 172 13 76 360 102 387 4.45e-16 80.1
MS.gene034500.t1 MTR_1g086350 26.667 255 126 9 82 316 19 232 4.56e-16 78.2
MS.gene034500.t1 MTR_4g037470 26.667 315 172 13 76 360 93 378 4.88e-16 79.7
MS.gene034500.t1 MTR_2g078760 28.340 247 124 9 79 317 38 239 6.70e-16 77.4
MS.gene034500.t1 MTR_2g078760 27.018 285 149 11 46 317 80 318 1.38e-15 77.4
MS.gene034500.t1 MTR_4g094208 26.354 277 153 10 54 321 32 266 3.80e-15 75.5
MS.gene034500.t1 MTR_4g116420 26.645 304 155 12 65 355 105 353 5.69e-15 76.3
MS.gene034500.t1 MTR_3g104710 25.828 302 160 9 79 359 286 544 1.19e-14 75.9
MS.gene034500.t1 MTR_2g435550 27.458 295 164 10 76 351 75 338 5.74e-14 72.8
MS.gene034500.t1 MTR_2g435550 27.458 295 164 10 76 351 89 352 6.52e-14 72.8
MS.gene034500.t1 MTR_2g090190 26.646 319 176 12 76 365 87 376 9.15e-14 72.8
MS.gene034500.t1 MTR_4g123080 28.125 256 131 10 77 316 93 311 2.08e-13 71.2
MS.gene034500.t1 MTR_4g123080 28.125 256 131 10 77 316 111 329 2.85e-13 70.9
MS.gene034500.t1 MTR_4g007440 25.094 267 135 8 79 305 52 293 1.17e-12 68.9
MS.gene034500.t1 MTR_7g112490 23.026 304 177 11 76 352 106 379 2.61e-12 68.2
MS.gene034500.t1 MTR_7g112490 23.026 304 177 11 76 352 88 361 2.96e-12 67.8
MS.gene034500.t1 MTR_1g015110 26.578 301 163 13 79 360 290 551 4.06e-12 68.2
MS.gene034500.t1 MTR_2g435550 27.099 262 155 8 76 318 89 333 4.09e-12 67.4
MS.gene034500.t1 MTR_1g050520 24.852 338 180 14 33 352 363 644 5.20e-12 67.8
MS.gene034500.t1 MTR_2g008850 26.172 256 138 11 73 321 13 224 5.22e-12 65.5
MS.gene034500.t1 MTR_1g028300 28.287 251 144 8 74 324 140 354 5.53e-12 67.0
MS.gene034500.t1 MTR_2g008850 25.532 282 153 13 53 321 33 270 9.73e-12 65.5
MS.gene034500.t1 MTR_8g463130 27.273 242 127 12 82 317 41 239 1.09e-11 65.1
MS.gene034500.t1 MTR_8g463130 27.273 242 127 12 82 317 69 267 1.31e-11 65.1
MS.gene034500.t1 MTR_5g063940 24.675 308 160 12 53 350 34 279 1.62e-11 64.7
MS.gene034500.t1 MTR_5g080680 24.891 229 134 6 79 300 132 329 1.95e-11 65.5
MS.gene034500.t1 MTR_8g017240 24.823 282 173 10 76 342 83 340 7.70e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034500.t1 AT5G06750 69.565 391 111 3 1 383 1 391 0.0 555
MS.gene034500.t1 AT5G06750 69.565 391 111 3 1 383 1 391 0.0 555
MS.gene034500.t1 AT5G06750 71.378 283 76 2 106 383 27 309 1.54e-145 415
MS.gene034500.t1 AT3G51370 53.034 379 160 4 1 370 1 370 1.92e-145 417
MS.gene034500.t1 AT3G51370 53.034 379 160 4 1 370 1 370 1.92e-145 417
MS.gene034500.t1 AT5G66080 52.094 382 165 4 1 370 1 376 8.17e-143 411
MS.gene034500.t1 AT4G38520 50.919 381 170 5 1 370 1 375 5.18e-139 402
MS.gene034500.t1 AT4G38520 50.919 381 170 5 1 370 1 375 5.18e-139 402
MS.gene034500.t1 AT3G12620 50.781 384 173 4 5 378 7 384 3.83e-138 399
MS.gene034500.t1 AT3G12620 50.781 384 173 4 5 378 7 384 3.83e-138 399
MS.gene034500.t1 AT3G17090 56.918 318 136 1 40 356 45 362 5.78e-137 396
MS.gene034500.t1 AT3G55050 52.676 355 155 3 7 352 8 358 4.11e-135 391
MS.gene034500.t1 AT3G55050 52.676 355 155 3 7 352 8 358 4.11e-135 391
MS.gene034500.t1 AT4G33920 56.748 326 137 2 33 354 15 340 2.44e-134 389
MS.gene034500.t1 AT5G02760 53.198 344 152 2 35 370 20 362 3.38e-121 355
MS.gene034500.t1 AT5G66080 56.296 270 109 3 107 370 6 272 9.06e-109 320
MS.gene034500.t1 AT3G51370 55.849 265 115 2 107 370 22 285 5.99e-108 319
MS.gene034500.t1 AT3G17090 54.613 271 122 1 40 309 45 315 7.35e-108 320
MS.gene034500.t1 AT4G33920 57.143 252 104 2 107 354 6 257 1.90e-101 303
MS.gene034500.t1 AT4G33920 59.031 227 89 2 132 354 1 227 1.15e-94 284
MS.gene034500.t1 AT3G16560 29.808 312 177 5 78 352 174 480 5.40e-36 137
MS.gene034500.t1 AT3G16560 29.808 312 177 5 78 352 174 480 5.40e-36 137
MS.gene034500.t1 AT3G16560 29.808 312 177 5 78 352 174 480 5.40e-36 137
MS.gene034500.t1 AT5G02400 32.090 268 142 5 121 353 404 666 4.69e-34 134
MS.gene034500.t1 AT2G35350 27.759 299 160 6 105 352 477 770 2.16e-29 120
MS.gene034500.t1 AT2G40180 28.615 325 153 8 41 354 131 387 3.04e-29 117
MS.gene034500.t1 AT2G46920 26.129 310 158 6 110 353 532 836 3.28e-29 120
MS.gene034500.t1 AT2G46920 26.129 310 158 6 110 353 532 836 3.28e-29 120
MS.gene034500.t1 AT2G46920 26.129 310 158 6 110 353 532 836 3.28e-29 120
MS.gene034500.t1 AT2G46920 26.129 310 158 6 110 353 532 836 3.28e-29 120
MS.gene034500.t1 AT3G09400 29.104 268 149 6 118 353 384 642 6.36e-29 119
MS.gene034500.t1 AT1G07630 27.305 282 162 4 118 361 386 662 1.29e-28 118
MS.gene034500.t1 AT2G28890 27.682 289 163 5 114 361 371 654 1.65e-28 117
MS.gene034500.t1 AT3G16560 29.078 282 158 5 78 322 174 450 3.32e-28 115
MS.gene034500.t1 AT1G07160 31.071 280 138 9 77 354 152 378 5.57e-27 110
MS.gene034500.t1 AT1G67820 32.653 245 121 10 79 317 115 321 5.98e-26 108
MS.gene034500.t1 AT1G67820 32.530 249 124 10 75 317 147 357 9.08e-26 108
MS.gene034500.t1 AT2G30020 29.603 277 137 9 77 350 169 390 1.04e-23 102
MS.gene034500.t1 AT3G09400 28.270 237 129 6 118 322 384 611 1.44e-20 94.0
MS.gene034500.t1 AT4G31750 29.832 238 127 7 79 314 64 263 2.01e-20 91.3
MS.gene034500.t1 AT4G31750 29.832 238 127 7 79 314 64 263 2.01e-20 91.3
MS.gene034500.t1 AT4G31750 29.832 238 127 7 79 314 64 263 2.42e-20 90.9
MS.gene034500.t1 AT2G20630 26.471 272 153 11 54 318 34 265 6.47e-19 86.3
MS.gene034500.t1 AT2G20630 26.471 272 153 11 54 318 34 265 7.33e-19 86.7
MS.gene034500.t1 AT4G28400 26.820 261 147 12 64 321 31 250 1.25e-18 85.1
MS.gene034500.t1 AT4G28400 26.820 261 147 12 64 321 53 272 3.06e-18 84.7
MS.gene034500.t1 AT3G51470 26.774 310 158 11 64 365 47 295 3.14e-18 85.1
MS.gene034500.t1 AT3G51470 26.861 309 157 11 65 365 92 339 3.74e-18 85.5
MS.gene034500.t1 AT2G33700 29.297 256 134 9 69 316 113 329 4.02e-18 85.5
MS.gene034500.t1 AT5G53140 23.239 284 173 8 42 320 93 336 5.35e-18 85.5
MS.gene034500.t1 AT5G53140 23.239 284 173 8 42 320 93 336 5.35e-18 85.5
MS.gene034500.t1 AT5G24940 29.018 224 115 6 79 299 64 246 1.63e-17 84.3
MS.gene034500.t1 AT1G48040 27.304 293 160 11 35 321 90 335 2.01e-17 83.6
MS.gene034500.t1 AT5G10740 27.935 247 123 9 79 316 64 264 3.48e-17 82.4
MS.gene034500.t1 AT5G10740 27.935 247 123 9 79 316 64 264 3.48e-17 82.4
MS.gene034500.t1 AT3G62260 26.562 256 152 7 69 316 17 244 9.88e-17 80.5
MS.gene034500.t1 AT1G43900 26.829 246 130 8 74 313 149 350 2.11e-16 80.5
MS.gene034500.t1 AT3G62260 26.459 257 153 7 69 317 106 334 2.70e-16 80.1
MS.gene034500.t1 AT3G62260 26.459 257 153 7 69 317 107 335 2.72e-16 80.1
MS.gene034500.t1 AT4G26080 29.514 288 156 13 75 350 168 420 3.15e-16 80.5
MS.gene034500.t1 AT3G16800 28.333 300 149 13 76 351 90 347 3.16e-16 79.7
MS.gene034500.t1 AT3G16800 28.333 300 149 13 76 351 90 347 3.16e-16 79.7
MS.gene034500.t1 AT3G16800 28.333 300 149 13 76 351 90 347 3.16e-16 79.7
MS.gene034500.t1 AT3G16800 30.213 235 113 10 130 351 56 252 2.49e-15 75.9
MS.gene034500.t1 AT3G16800 30.213 235 113 10 130 351 56 252 2.49e-15 75.9
MS.gene034500.t1 AT2G25620 24.204 314 187 11 72 383 122 386 2.57e-15 77.4
MS.gene034500.t1 AT2G25620 24.204 314 187 11 72 383 122 386 2.57e-15 77.4
MS.gene034500.t1 AT5G26010 24.438 356 229 10 12 357 4 329 4.95e-15 75.9
MS.gene034500.t1 AT4G28400 27.434 226 123 10 64 288 53 238 1.86e-14 73.2
MS.gene034500.t1 AT1G03590 26.623 308 171 14 76 356 87 366 2.38e-14 74.7
MS.gene034500.t1 AT5G01700 26.144 306 176 13 76 363 26 299 1.04e-13 72.0
MS.gene034500.t1 AT3G17250 26.562 256 141 11 79 321 158 379 1.08e-13 72.4
MS.gene034500.t1 AT5G01700 26.144 306 176 13 76 363 75 348 1.95e-13 71.6
MS.gene034500.t1 AT5G36250 27.660 329 153 14 76 355 95 387 2.15e-13 71.6
MS.gene034500.t1 AT5G57050 28.475 295 147 15 77 350 158 409 3.53e-13 70.9
MS.gene034500.t1 AT4G08260 27.155 232 92 9 67 298 14 168 3.72e-13 68.6
MS.gene034500.t1 AT3G05640 26.280 293 165 11 76 351 90 348 4.50e-13 70.5
MS.gene034500.t1 AT3G05640 26.280 293 165 11 76 351 90 348 4.50e-13 70.5
MS.gene034500.t1 AT3G05640 26.280 293 165 11 76 351 90 348 4.50e-13 70.5
MS.gene034500.t1 AT5G57050 29.870 231 116 11 77 298 118 311 4.54e-13 70.5
MS.gene034500.t1 AT5G59220 29.310 232 116 11 79 299 148 342 4.99e-13 70.5
MS.gene034500.t1 AT4G03415 27.010 311 169 14 76 356 94 376 6.62e-13 70.5
MS.gene034500.t1 AT4G03415 27.010 311 169 14 76 356 94 376 6.62e-13 70.5
MS.gene034500.t1 AT4G03415 27.010 311 169 14 76 356 94 376 6.62e-13 70.5
MS.gene034500.t1 AT1G34750 24.675 308 160 12 53 350 34 279 1.21e-12 68.2
MS.gene034500.t1 AT1G34750 24.675 308 160 12 53 350 34 279 1.21e-12 68.2
MS.gene034500.t1 AT1G34750 24.675 308 160 12 53 350 34 279 1.21e-12 68.2
MS.gene034500.t1 AT1G22280 25.649 308 153 11 54 350 36 278 1.55e-12 67.8
MS.gene034500.t1 AT1G07430 25.000 244 116 7 79 300 157 355 1.83e-12 68.9
MS.gene034500.t1 AT4G31860 25.188 266 126 8 79 300 52 288 2.67e-12 67.4
MS.gene034500.t1 AT1G72770 25.490 306 166 10 79 359 238 506 4.69e-12 67.8
MS.gene034500.t1 AT1G72770 25.490 306 166 10 79 359 238 506 4.69e-12 67.8
MS.gene034500.t1 AT1G72770 25.490 306 166 10 79 359 238 506 4.69e-12 67.8
MS.gene034500.t1 AT4G31860 25.188 266 126 8 79 300 52 288 5.51e-12 67.0
MS.gene034500.t1 AT2G40860 25.559 313 167 14 50 352 294 550 7.59e-12 67.4
MS.gene034500.t1 AT2G40860 25.559 313 167 14 50 352 392 648 9.84e-12 67.0
MS.gene034500.t1 AT2G40860 26.071 280 161 12 50 318 392 636 1.23e-11 66.6
MS.gene034500.t1 AT4G32950 24.603 252 165 10 76 322 69 300 1.80e-11 65.1
MS.gene034500.t1 AT3G15260 25.993 277 137 10 79 350 73 286 5.74e-11 63.2
MS.gene034500.t1 AT3G15260 25.993 277 137 10 79 350 73 286 5.74e-11 63.2

Find 76 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCATTCATTGAGTTTCT+TGG 0.151665 1.1:-40742813 None:intergenic
TCTTCTGATCTCCTCTTTGC+AGG 0.247593 1.1:-40741315 None:intergenic
CATGGAACATGGCGCATTAA+AGG 0.259091 1.1:+40741386 MS.gene034500:CDS
TTTGCAGGCATTATGCTCTT+TGG 0.273523 1.1:-40741300 None:intergenic
GCAGGGATTGCTAAAGAAAA+TGG 0.288402 1.1:+40741050 MS.gene034500:intron
ACAACAATTTGTGAATCTTC+TGG 0.288612 1.1:-40741356 None:intergenic
AACATCAAACATACAGTTCT+AGG 0.297305 1.1:-40742442 None:intergenic
GTTGTCTTGTTGGTGTTATC+TGG 0.300700 1.1:+40741186 MS.gene034500:CDS
TATTTGCATCTGATGGCCTT+TGG 0.352622 1.1:+40742374 MS.gene034500:CDS
TTAACAAACTTAGAAGCTTC+AGG 0.354933 1.1:-40739088 None:intergenic
ATTTGCATCTGATGGCCTTT+GGG 0.364124 1.1:+40742375 MS.gene034500:CDS
ACTCTTGTTCGAAGGAGCTT+TGG 0.367886 1.1:+40741131 MS.gene034500:CDS
CTATCTCCGAAGATATTATA+AGG 0.376254 1.1:+40741075 MS.gene034500:CDS
AATATAACAAATTTATCGTT+AGG 0.381864 1.1:-40742355 None:intergenic
GAGTGCAAGAAGACTTCTTC+AGG 0.385692 1.1:+40742582 MS.gene034500:CDS
TGTGTCAATAAACCCTTTGA+TGG 0.386707 1.1:-40742764 None:intergenic
AAAACTCTTGAACACATAGA+TGG 0.402165 1.1:-40742328 None:intergenic
ATGGGTTCATGTTGTCTTGT+TGG 0.407205 1.1:+40741176 MS.gene034500:CDS
CATCTGAAGATGCTCTTGTT+TGG 0.409250 1.1:+40738927 MS.gene034500:CDS
CGTGCTGTGATCGGTTCAAC+TGG 0.411906 1.1:+40741245 MS.gene034500:CDS
AGCATTCCTTATAATATCTT+CGG 0.421473 1.1:-40741081 None:intergenic
AGATTTGCAGAAGGTTGAAA+AGG 0.421638 1.1:+40742648 MS.gene034500:CDS
GATCATAGTCAAGTTGAAAC+TGG 0.428878 1.1:+40739022 MS.gene034500:CDS
CAAATTGTTGTTATGAAACA+TGG 0.429867 1.1:+40741368 MS.gene034500:CDS
TGAATGAGGCAGCAAGGAAA+AGG 0.452832 1.1:+40742611 MS.gene034500:CDS
GGAGATTCTCGTGCTGTGAT+CGG 0.462599 1.1:+40741236 MS.gene034500:CDS
AGAAGGTTGAAAAGGGAATT+CGG 0.464125 1.1:+40742656 MS.gene034500:CDS
GATTTGCAGAAGGTTGAAAA+GGG 0.465611 1.1:+40742649 MS.gene034500:CDS
GATAACACAGGTCGGCTGAT+AGG 0.467496 1.1:-40742298 None:intergenic
ACACTTCATATCGCTAATCT+TGG 0.471882 1.1:+40741215 MS.gene034500:CDS
GCTGTTTCTGCTACGGAGGA+TGG 0.472253 1.1:+40741101 MS.gene034500:CDS
CTTGTTGGTGTTATCTGGAA+AGG 0.472649 1.1:+40741191 MS.gene034500:CDS
CTTCAAATATGCATCTCCTA+TGG 0.476245 1.1:-40742224 None:intergenic
TTTGTTATATTTGCATCTGA+TGG 0.477307 1.1:+40742367 MS.gene034500:CDS
TTCCTGAGTTATCCATCAAA+GGG 0.477943 1.1:+40742752 MS.gene034500:CDS
TCAAGCTAATGAAGTAATTG+AGG 0.480948 1.1:+40739000 MS.gene034500:CDS
ATTCGTGCATAGCGTCGAAC+TGG 0.485130 1.1:-40738866 None:intergenic
GTTGGTGTTTATGATGGTCA+TGG 0.489495 1.1:+40739061 MS.gene034500:CDS
ACAGGTCGGCTGATAGGATC+AGG 0.492423 1.1:-40742292 None:intergenic
TTGGGAACACATGACAAATC+AGG 0.500543 1.1:+40742393 MS.gene034500:CDS
AAAGGGTTTATTGACACAGT+TGG 0.502286 1.1:+40742769 MS.gene034500:CDS
AAGTGGAACCGCGGGAATGA+AGG 0.509280 1.1:-40742268 None:intergenic
GTTCCTGAGTTATCCATCAA+AGG 0.511493 1.1:+40742751 MS.gene034500:CDS
GATTTCTGACTCTTGTTCGA+AGG 0.521358 1.1:+40741123 MS.gene034500:CDS
TCTTCAGGCTGCTTTGAATG+AGG 0.522502 1.1:+40742597 MS.gene034500:CDS
AAGGAATGCTGTTTCTGCTA+CGG 0.533195 1.1:+40741094 MS.gene034500:CDS
GAATGAGGCAGCAAGGAAAA+GGG 0.539011 1.1:+40742612 MS.gene034500:CDS
TTGTTGGTGTTATCTGGAAA+GGG 0.539840 1.1:+40741192 MS.gene034500:CDS
AAAGCCGCTGATAGCAGCAA+TGG 0.553063 1.1:+40741157 MS.gene034500:CDS
ACATCAAACATACAGTTCTA+GGG 0.557511 1.1:-40742441 None:intergenic
GCGCATTAAAGGCATCATCC+AGG 0.559771 1.1:+40741397 MS.gene034500:CDS
AAGCCGCTGATAGCAGCAAT+GGG 0.565319 1.1:+40741158 MS.gene034500:CDS
AGATGCATATTTGAAGCGGC+CGG 0.571705 1.1:+40742231 MS.gene034500:CDS
GGCTGATAGGATCAGGCAAG+TGG 0.577398 1.1:-40742285 None:intergenic
GATCAGGCAAGTGGAACCGC+GGG 0.583734 1.1:-40742276 None:intergenic
GAGATATAAAGATTTGCAGA+AGG 0.584024 1.1:+40742639 MS.gene034500:CDS
CTGCTTTGAATGAGGCAGCA+AGG 0.594845 1.1:+40742605 MS.gene034500:CDS
GAATGCTGTTTCTGCTACGG+AGG 0.597657 1.1:+40741097 MS.gene034500:CDS
ATTAGCTTGAACAATAGCAA+AGG 0.603217 1.1:-40738987 None:intergenic
GAAGGATCAAATGAGAACTC+CGG 0.604037 1.1:-40742250 None:intergenic
GATCAGGTATCAAAATCCAT+AGG 0.620151 1.1:+40742208 MS.gene034500:intron
CACCACAAGAGTGTTTCTCA+AGG 0.621992 1.1:-40738958 None:intergenic
GAACCCATTGCTGCTATCAG+CGG 0.622130 1.1:-40741161 None:intergenic
GATTTGTCATGTGTTCCCAA+AGG 0.627452 1.1:-40742390 None:intergenic
CATTTGATCCTTCATTCCCG+CGG 0.631409 1.1:+40742260 MS.gene034500:CDS
GACCTTGAGAAACACTCTTG+TGG 0.632718 1.1:+40738956 MS.gene034500:CDS
TAGGAGATGCATATTTGAAG+CGG 0.637442 1.1:+40742227 MS.gene034500:CDS
AACCCTTTGATGGATAACTC+AGG 0.640358 1.1:-40742754 None:intergenic
AGAGCATAATGCCTGCAAAG+AGG 0.641485 1.1:+40741304 MS.gene034500:CDS
GGATCAGGCAAGTGGAACCG+CGG 0.643319 1.1:-40742277 None:intergenic
GATGGTTCTGCTGATAACAC+AGG 0.665211 1.1:-40742310 None:intergenic
TTGTTATGAAACATGGAACA+TGG 0.679272 1.1:+40741375 MS.gene034500:CDS
GGTGTTTATGATGGTCATGG+TGG 0.696441 1.1:+40739064 MS.gene034500:CDS
GTTCTGCTGATAACACAGGT+CGG 0.711534 1.1:-40742306 None:intergenic
CAACCACTGTGATATCATCG+TGG 0.720113 1.1:-40742687 None:intergenic
TTTCCACGATGATATCACAG+TGG 0.728364 1.1:+40742684 MS.gene034500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATATATATTTTTTTTT+TGG + chr1.1:40742004-40742023 MS.gene034500:intron 0.0%
!! CATATAAGATATAAAATTAA+GGG - chr1.1:40740335-40740354 None:intergenic 10.0%
!! TCATATAAGATATAAAATTA+AGG - chr1.1:40740336-40740355 None:intergenic 10.0%
!!! AATTTTATATCTTATATGAA+AGG + chr1.1:40740337-40740356 MS.gene034500:intron 10.0%
!!! ATATATTTTGTTGAAAATTT+GGG + chr1.1:40739579-40739598 MS.gene034500:intron 10.0%
!!! CATTCTTATAATTAATTTTA+GGG + chr1.1:40740128-40740147 MS.gene034500:intron 10.0%
!!! TATATATTTTGTTGAAAATT+TGG + chr1.1:40739578-40739597 MS.gene034500:intron 10.0%
!!! TTGTTTTGTATTAAATAATA+TGG + chr1.1:40739634-40739653 MS.gene034500:intron 10.0%
!!! TTTTTTTAATGTTGAATAAA+TGG + chr1.1:40739397-40739416 MS.gene034500:intron 10.0%
!! AATATAACAAATTTATCGTT+AGG - chr1.1:40742358-40742377 None:intergenic 15.0%
!! ATACTAATTACTAAACTTAA+AGG - chr1.1:40740836-40740855 None:intergenic 15.0%
!! ATCATTATTAGTTAAATTCA+TGG - chr1.1:40742117-40742136 None:intergenic 15.0%
!! TACTAATTACTAAACTTAAA+GGG - chr1.1:40740835-40740854 None:intergenic 15.0%
!! TTGTATTAAATAATATGGTA+TGG + chr1.1:40739639-40739658 MS.gene034500:intron 15.0%
!! TTTACTATAATAACACAATT+TGG + chr1.1:40740537-40740556 MS.gene034500:intron 15.0%
!!! AACTCTTTTTTTTTAAAACT+CGG - chr1.1:40740055-40740074 None:intergenic 15.0%
!!! GCATTCTTATAATTAATTTT+AGG + chr1.1:40740127-40740146 MS.gene034500:intron 15.0%
!!! TAAACTTTGAAAATTACTTA+TGG - chr1.1:40740613-40740632 None:intergenic 15.0%
!! AATTCATGTTCAAAATAGAT+AGG - chr1.1:40741790-40741809 None:intergenic 20.0%
!! ACTTAACATAAAAAAACATG+TGG - chr1.1:40740912-40740931 None:intergenic 20.0%
!! CAAAATATTTAACCATGTAA+TGG - chr1.1:40741602-40741621 None:intergenic 20.0%
!! TGACAAAATCAAATACAATA+AGG - chr1.1:40741693-40741712 None:intergenic 20.0%
!!! CTATACAGTTTTTCTTAAAA+AGG + chr1.1:40741517-40741536 MS.gene034500:intron 20.0%
!!! CTTTTTAAGAAAAACTGTAT+AGG - chr1.1:40741519-40741538 None:intergenic 20.0%
!!! TGTTTATTTCTTTAACATCA+GGG + chr1.1:40742559-40742578 MS.gene034500:intron 20.0%
!!! TTGATCAATGTTTGATATTT+TGG + chr1.1:40739708-40739727 MS.gene034500:intron 20.0%
!!! TTTATTTTCTCTTATTCCTA+TGG + chr1.1:40740882-40740901 MS.gene034500:intron 20.0%
!!! TTTTATTCTAACAATGAACT+TGG + chr1.1:40740103-40740122 MS.gene034500:intron 20.0%
!!! TTTTTAAGTAATAAGTGACT+TGG - chr1.1:40741989-40742008 None:intergenic 20.0%
! AACTGTTCAACCAATTAATT+GGG - chr1.1:40739512-40739531 None:intergenic 25.0%
! AAGTGATCTATAAGAAGTTA+AGG + chr1.1:40741005-40741024 MS.gene034500:intron 25.0%
! ACTAAAAATGTAATCATTGC+TGG - chr1.1:40740961-40740980 None:intergenic 25.0%
! AGCATTCCTTATAATATCTT+CGG - chr1.1:40741084-40741103 None:intergenic 25.0%
! ATTGGTTGAACAGTTAAATT+TGG + chr1.1:40739517-40739536 MS.gene034500:intron 25.0%
! TAAAACATCTTAGACAAAGT+AGG + chr1.1:40739422-40739441 MS.gene034500:intron 25.0%
! TAACTGTTCAACCAATTAAT+TGG - chr1.1:40739513-40739532 None:intergenic 25.0%
! TCCAAGTTACAAATTCTAAA+AGG - chr1.1:40740781-40740800 None:intergenic 25.0%
! TGGTTCATGTATATAGAAAA+AGG - chr1.1:40741633-40741652 None:intergenic 25.0%
! TTTATGTTACCTTTATCGTT+TGG + chr1.1:40741465-40741484 MS.gene034500:intron 25.0%
!! CAAATTGTTGTTATGAAACA+TGG + chr1.1:40741368-40741387 MS.gene034500:CDS 25.0%
!! CTGTTTATTTCTTTAACATC+AGG + chr1.1:40742558-40742577 MS.gene034500:intron 25.0%
!! GTTTATTTCTTTAACATCAG+GGG + chr1.1:40742560-40742579 MS.gene034500:intron 25.0%
!! TGGAAACTTCTAAATTTTGA+TGG - chr1.1:40742796-40742815 None:intergenic 25.0%
!! TTTGTTATATTTGCATCTGA+TGG + chr1.1:40742367-40742386 MS.gene034500:CDS 25.0%
!!! ACCTTTTAGAATTTGTAACT+TGG + chr1.1:40740777-40740796 MS.gene034500:intron 25.0%
!!! AGCTCTTTTTTGTTGTTATT+TGG + chr1.1:40739754-40739773 MS.gene034500:intron 25.0%
!!! CTTTTTTGTTGTTATTTGGA+TGG + chr1.1:40739758-40739777 MS.gene034500:intron 25.0%
!!! GACATATTGTTTTCTAAGAA+AGG + chr1.1:40739352-40739371 MS.gene034500:intron 25.0%
AAAACTCTTGAACACATAGA+TGG - chr1.1:40742331-40742350 None:intergenic 30.0%
AAACATAATTGTAGGATCCT+AGG - chr1.1:40742492-40742511 None:intergenic 30.0%
AAATTCACTATGCACATCTT+TGG + chr1.1:40740408-40740427 MS.gene034500:intron 30.0%
AACATCAAACATACAGTTCT+AGG - chr1.1:40742445-40742464 None:intergenic 30.0%
AAGCAAAAACCAAACGATAA+AGG - chr1.1:40741477-40741496 None:intergenic 30.0%
AAGGGAATTATTACCACAAA+AGG - chr1.1:40740267-40740286 None:intergenic 30.0%
AATTTGTAACTTGGAAAGCT+CGG + chr1.1:40740786-40740805 MS.gene034500:intron 30.0%
ACAACAATTTGTGAATCTTC+TGG - chr1.1:40741359-40741378 None:intergenic 30.0%
ACATCAAACATACAGTTCTA+GGG - chr1.1:40742444-40742463 None:intergenic 30.0%
ACTGGTTAAGGTTATGAAAA+AGG + chr1.1:40740176-40740195 MS.gene034500:intron 30.0%
AGTGGTTCATTAGAATGAAT+CGG - chr1.1:40741865-40741884 None:intergenic 30.0%
ATAAGATTCGCTTCACAATT+CGG + chr1.1:40742048-40742067 MS.gene034500:intron 30.0%
ATGTGTTGAATTTCATTGCA+GGG + chr1.1:40741033-40741052 MS.gene034500:intron 30.0%
CTATCTCCGAAGATATTATA+AGG + chr1.1:40741075-40741094 MS.gene034500:CDS 30.0%
GAACTTATCTCCTAAAACAA+AGG - chr1.1:40740713-40740732 None:intergenic 30.0%
GAGAAAGTAATATGAAAACC+TGG - chr1.1:40741418-40741437 None:intergenic 30.0%
GAGATATAAAGATTTGCAGA+AGG + chr1.1:40742639-40742658 MS.gene034500:CDS 30.0%
GGGTTAAACATACCATAAAA+GGG - chr1.1:40740285-40740304 None:intergenic 30.0%
TATGTGTTGAATTTCATTGC+AGG + chr1.1:40741032-40741051 MS.gene034500:intron 30.0%
TCAAGCTAATGAAGTAATTG+AGG + chr1.1:40739000-40739019 MS.gene034500:CDS 30.0%
TTAACAAACTTAGAAGCTTC+AGG - chr1.1:40739091-40739110 None:intergenic 30.0%
TTGAACATGAATTTCGTCTT+AGG + chr1.1:40741797-40741816 MS.gene034500:intron 30.0%
TTGTTATGAAACATGGAACA+TGG + chr1.1:40741375-40741394 MS.gene034500:CDS 30.0%
! ATTAGCTTGAACAATAGCAA+AGG - chr1.1:40738990-40739009 None:intergenic 30.0%
! GTGGTAATAATTCCCTTTTA+TGG + chr1.1:40740270-40740289 MS.gene034500:intron 30.0%
! TCAAAAACAGTTTCCTTTTG+TGG + chr1.1:40740251-40740270 MS.gene034500:intron 30.0%
! TCTTATATGAAAGGTTTGCT+TGG + chr1.1:40740346-40740365 MS.gene034500:intron 30.0%
!! AATTTTAGGGAAATGCTAAC+CGG + chr1.1:40740141-40740160 MS.gene034500:intron 30.0%
!! GTTTTTGTTGGTGTTTATGA+TGG + chr1.1:40739055-40739074 MS.gene034500:CDS 30.0%
!!! TTTACACAAGCCTTTGTTTT+AGG + chr1.1:40740700-40740719 MS.gene034500:intron 30.0%
!!! TTTTTTTAAAACTCGGTGTC+CGG - chr1.1:40740048-40740067 None:intergenic 30.0%
AAAAACATGTGGCAATCCAT+AGG - chr1.1:40740901-40740920 None:intergenic 35.0%
AAAACATCGCTTTCATGACT+AGG + chr1.1:40739550-40739569 MS.gene034500:intron 35.0%
AAAGGGTTTATTGACACAGT+TGG + chr1.1:40742769-40742788 MS.gene034500:CDS 35.0%
AAATGAATGAGAGCCAAATG+GGG + chr1.1:40740560-40740579 MS.gene034500:intron 35.0%
AAGGGAGAATTTGGGAAATT+TGG + chr1.1:40740381-40740400 MS.gene034500:intron 35.0%
ACACTTCATATCGCTAATCT+TGG + chr1.1:40741215-40741234 MS.gene034500:CDS 35.0%
AGAAGGTTGAAAAGGGAATT+CGG + chr1.1:40742656-40742675 MS.gene034500:CDS 35.0%
AGATTTGCAGAAGGTTGAAA+AGG + chr1.1:40742648-40742667 MS.gene034500:CDS 35.0%
ATAACTCTGTATGGACATCA+AGG - chr1.1:40739952-40739971 None:intergenic 35.0%
CAAAGTAGGTTTATGAGTCA+CGG + chr1.1:40739436-40739455 MS.gene034500:intron 35.0%
CGGGTTAAACATACCATAAA+AGG - chr1.1:40740286-40740305 None:intergenic 35.0%
CTTCAAATATGCATCTCCTA+TGG - chr1.1:40742227-40742246 None:intergenic 35.0%
GAAATGAATGAGAGCCAAAT+GGG + chr1.1:40740559-40740578 MS.gene034500:intron 35.0%
GATCAGGTATCAAAATCCAT+AGG + chr1.1:40742208-40742227 MS.gene034500:intron 35.0%
GATTTGCAGAAGGTTGAAAA+GGG + chr1.1:40742649-40742668 MS.gene034500:CDS 35.0%
GCACGCTAAAACATAATTGT+AGG - chr1.1:40742500-40742519 None:intergenic 35.0%
GCTACACTACATTTAAGTAG+TGG - chr1.1:40741883-40741902 None:intergenic 35.0%
GTGGTTCATTAGAATGAATC+GGG - chr1.1:40741864-40741883 None:intergenic 35.0%
GTGTTTCAACTTCCATTACA+TGG + chr1.1:40741587-40741606 MS.gene034500:intron 35.0%
TAACTCTGTATGGACATCAA+GGG - chr1.1:40739951-40739970 None:intergenic 35.0%
TAGGAGATGCATATTTGAAG+CGG + chr1.1:40742227-40742246 MS.gene034500:CDS 35.0%
TCAAATGCAGCTTCAAACTA+TGG - chr1.1:40741653-40741672 None:intergenic 35.0%
TCTAAGAAAGGCTAGTATGA+AGG + chr1.1:40739364-40739383 MS.gene034500:intron 35.0%
TGATGAAATGCATTGTGTGT+TGG + chr1.1:40742133-40742152 MS.gene034500:intron 35.0%
TGTGTCAATAAACCCTTTGA+TGG - chr1.1:40742767-40742786 None:intergenic 35.0%
TGTTCAACCAATTAATTGGG+CGG - chr1.1:40739509-40739528 None:intergenic 35.0%
TTCCTGAGTTATCCATCAAA+GGG + chr1.1:40742752-40742771 MS.gene034500:CDS 35.0%
! CATTCTTCTTTTGCATCTCA+TGG - chr1.1:40742726-40742745 None:intergenic 35.0%
! GAGTTTTTCTCTTTCACATC+AGG + chr1.1:40739917-40739936 MS.gene034500:intron 35.0%
!! AGTTATGCTCTGGCTTTAAA+TGG + chr1.1:40739966-40739985 MS.gene034500:intron 35.0%
!! GATCATAGTCAAGTTGAAAC+TGG + chr1.1:40739022-40739041 MS.gene034500:CDS 35.0%
!! GTTATGCTCTGGCTTTAAAT+GGG + chr1.1:40739967-40739986 MS.gene034500:intron 35.0%
!! TTATGCTCTGGCTTTAAATG+GGG + chr1.1:40739968-40739987 MS.gene034500:intron 35.0%
!! TTGTTGGTGTTATCTGGAAA+GGG + chr1.1:40741192-40741211 MS.gene034500:CDS 35.0%
AAATTAAGGGTCCTGCTAAC+CGG - chr1.1:40740322-40740341 None:intergenic 40.0%
AACAAACATGTGTCAGAGAC+CGG - chr1.1:40739204-40739223 None:intergenic 40.0%
AACTCTGTATGGACATCAAG+GGG - chr1.1:40739950-40739969 None:intergenic 40.0%
AATGAATGAGAGCCAAATGG+GGG + chr1.1:40740561-40740580 MS.gene034500:intron 40.0%
ACTGTATAGGCACAACACAA+TGG - chr1.1:40741506-40741525 None:intergenic 40.0%
ATCTGTGTCTCATACATGTC+TGG + chr1.1:40739291-40739310 MS.gene034500:intron 40.0%
ATGGGTTCATGTTGTCTTGT+TGG + chr1.1:40741176-40741195 MS.gene034500:CDS 40.0%
ATTTGCATCTGATGGCCTTT+GGG + chr1.1:40742375-40742394 MS.gene034500:CDS 40.0%
CATAATTGTAGGATCCTAGG+TGG - chr1.1:40742489-40742508 None:intergenic 40.0%
CATCTGAAGATGCTCTTGTT+TGG + chr1.1:40738927-40738946 MS.gene034500:CDS 40.0%
CTTTGGCTATGCATAACTAC+AGG + chr1.1:40740425-40740444 MS.gene034500:intron 40.0%
GAAGGATCAAATGAGAACTC+CGG - chr1.1:40742253-40742272 None:intergenic 40.0%
GATCAAACCGCCCAATTAAT+TGG + chr1.1:40739499-40739518 MS.gene034500:intron 40.0%
GATTTCTGACTCTTGTTCGA+AGG + chr1.1:40741123-40741142 MS.gene034500:CDS 40.0%
GATTTGTCATGTGTTCCCAA+AGG - chr1.1:40742393-40742412 None:intergenic 40.0%
GCAGGGATTGCTAAAGAAAA+TGG + chr1.1:40741050-40741069 MS.gene034500:intron 40.0%
GGAAATGAATGAGAGCCAAA+TGG + chr1.1:40740558-40740577 MS.gene034500:intron 40.0%
GGGATGAATCATAGTGAATC+AGG - chr1.1:40741844-40741863 None:intergenic 40.0%
GTTCCTGAGTTATCCATCAA+AGG + chr1.1:40742751-40742770 MS.gene034500:CDS 40.0%
TATTTGCATCTGATGGCCTT+TGG + chr1.1:40742374-40742393 MS.gene034500:CDS 40.0%
TTCTTCTTCACACCACAACT+GGG + chr1.1:40742186-40742205 MS.gene034500:intron 40.0%
TTGGGAACACATGACAAATC+AGG + chr1.1:40742393-40742412 MS.gene034500:CDS 40.0%
TTTCCACGATGATATCACAG+TGG + chr1.1:40742684-40742703 MS.gene034500:CDS 40.0%
TTTCTTCTTCACACCACAAC+TGG + chr1.1:40742185-40742204 MS.gene034500:intron 40.0%
TTTGCAGGCATTATGCTCTT+TGG - chr1.1:40741303-40741322 None:intergenic 40.0%
! AACCCTTTGATGGATAACTC+AGG - chr1.1:40742757-40742776 None:intergenic 40.0%
! GTAGTGGAAAGGGAGAATTT+GGG + chr1.1:40740373-40740392 MS.gene034500:intron 40.0%
! GTTGGTGTTTATGATGGTCA+TGG + chr1.1:40739061-40739080 MS.gene034500:CDS 40.0%
! TGTAGTGGAAAGGGAGAATT+TGG + chr1.1:40740372-40740391 MS.gene034500:intron 40.0%
!! AAGGAATGCTGTTTCTGCTA+CGG + chr1.1:40741094-40741113 MS.gene034500:CDS 40.0%
!! ATCATCTTTTTCGTCCACCT+AGG + chr1.1:40742472-40742491 MS.gene034500:intron 40.0%
!! CTTGTTGGTGTTATCTGGAA+AGG + chr1.1:40741191-40741210 MS.gene034500:CDS 40.0%
!! GTTGTCTTGTTGGTGTTATC+TGG + chr1.1:40741186-40741205 MS.gene034500:CDS 40.0%
!! TGTCTGTTTCTGTGCTTCAT+AGG + chr1.1:40739322-40739341 MS.gene034500:intron 40.0%
!!! GGTTCTGATGCTGTTTTTGT+TGG + chr1.1:40739043-40739062 MS.gene034500:CDS 40.0%
ACTCTTGTTCGAAGGAGCTT+TGG + chr1.1:40741131-40741150 MS.gene034500:CDS 45.0%
AGAGCATAATGCCTGCAAAG+AGG + chr1.1:40741304-40741323 MS.gene034500:CDS 45.0%
AGCCAGAGCATAACTCTGTA+TGG - chr1.1:40739961-40739980 None:intergenic 45.0%
CAACCACTGTGATATCATCG+TGG - chr1.1:40742690-40742709 None:intergenic 45.0%
CATGGAACATGGCGCATTAA+AGG + chr1.1:40741386-40741405 MS.gene034500:CDS 45.0%
CATTTGATCCTTCATTCCCG+CGG + chr1.1:40742260-40742279 MS.gene034500:CDS 45.0%
CGCTTCACAATTCGGAAAGT+AGG + chr1.1:40742056-40742075 MS.gene034500:intron 45.0%
CTATCACGCTATCTGATCGA+AGG + chr1.1:40739173-40739192 MS.gene034500:intron 45.0%
GAAGCACAGAAACAGACACA+CGG - chr1.1:40739320-40739339 None:intergenic 45.0%
GAATGAGGCAGCAAGGAAAA+GGG + chr1.1:40742612-40742631 MS.gene034500:CDS 45.0%
GACCTTGAGAAACACTCTTG+TGG + chr1.1:40738956-40738975 MS.gene034500:CDS 45.0%
GAGTGCAAGAAGACTTCTTC+AGG + chr1.1:40742582-40742601 MS.gene034500:CDS 45.0%
GATGTTACCAATCCCCCATT+TGG - chr1.1:40740576-40740595 None:intergenic 45.0%
GGTGTTTATGATGGTCATGG+TGG + chr1.1:40739064-40739083 MS.gene034500:CDS 45.0%
GTCCATACAGAGTTATGCTC+TGG + chr1.1:40739956-40739975 MS.gene034500:intron 45.0%
GTTATTTGGATGGCCCTTTG+TGG + chr1.1:40739768-40739787 MS.gene034500:intron 45.0%
GTTCTGCTGATAACACAGGT+CGG - chr1.1:40742309-40742328 None:intergenic 45.0%
TCTTCTGATCTCCTCTTTGC+AGG - chr1.1:40741318-40741337 None:intergenic 45.0%
TGAATGAGGCAGCAAGGAAA+AGG + chr1.1:40742611-40742630 MS.gene034500:CDS 45.0%
TTGATACCTGATCCCAGTTG+TGG - chr1.1:40742201-40742220 None:intergenic 45.0%
! AGATGCATATTTGAAGCGGC+CGG + chr1.1:40742231-40742250 MS.gene034500:CDS 45.0%
! AGGTTTGCTTGGTTGTGTAG+TGG + chr1.1:40740357-40740376 MS.gene034500:intron 45.0%
! GATGGTTCTGCTGATAACAC+AGG - chr1.1:40742313-40742332 None:intergenic 45.0%
! GCTTGGTTGTGTAGTGGAAA+GGG + chr1.1:40740363-40740382 MS.gene034500:intron 45.0%
! TCTTCAGGCTGCTTTGAATG+AGG + chr1.1:40742597-40742616 MS.gene034500:CDS 45.0%
! TGCTTGGTTGTGTAGTGGAA+AGG + chr1.1:40740362-40740381 MS.gene034500:intron 45.0%
!! CACCACAAGAGTGTTTCTCA+AGG - chr1.1:40738961-40738980 None:intergenic 45.0%
AAAGCCGCTGATAGCAGCAA+TGG + chr1.1:40741157-40741176 MS.gene034500:CDS 50.0%
AAGCCGCTGATAGCAGCAAT+GGG + chr1.1:40741158-40741177 MS.gene034500:CDS 50.0%
ATAACCTTAACCAGTGCCCC+GGG - chr1.1:40740171-40740190 None:intergenic 50.0%
ATGAGAGCCAAATGGGGGAT+TGG + chr1.1:40740566-40740585 MS.gene034500:intron 50.0%
ATTGGACCCCTCAGATGAGT+CGG + chr1.1:40740014-40740033 MS.gene034500:intron 50.0%
CATAACCTTAACCAGTGCCC+CGG - chr1.1:40740172-40740191 None:intergenic 50.0%
GAACCCATTGCTGCTATCAG+CGG - chr1.1:40741164-40741183 None:intergenic 50.0%
GATAACACAGGTCGGCTGAT+AGG - chr1.1:40742301-40742320 None:intergenic 50.0%
GGAGATTCTCGTGCTGTGAT+CGG + chr1.1:40741236-40741255 MS.gene034500:CDS 50.0%
TTCACACCACAACTGGGATC+AGG + chr1.1:40742192-40742211 MS.gene034500:intron 50.0%
! ATTCGTGCATAGCGTCGAAC+TGG - chr1.1:40738869-40738888 None:intergenic 50.0%
! CTGCTTTGAATGAGGCAGCA+AGG + chr1.1:40742605-40742624 MS.gene034500:CDS 50.0%
! TATCTGATCGAAGGCGTGTC+CGG + chr1.1:40739182-40739201 MS.gene034500:intron 50.0%
!! GAATGCTGTTTCTGCTACGG+AGG + chr1.1:40741097-40741116 MS.gene034500:CDS 50.0%
!! GCGCATTAAAGGCATCATCC+AGG + chr1.1:40741397-40741416 MS.gene034500:CDS 50.0%
!! GGCTGGCTGGTTGATTTGTT+TGG + chr1.1:40739795-40739814 MS.gene034500:intron 50.0%
AAGTGGAACCGCGGGAATGA+AGG - chr1.1:40742271-40742290 None:intergenic 55.0%
ACAGGTCGGCTGATAGGATC+AGG - chr1.1:40742295-40742314 None:intergenic 55.0%
CAGCCAGCCTGATCCACAAA+GGG - chr1.1:40739784-40739803 None:intergenic 55.0%
CCAATCCCACCGTTCACTTG+CGG - chr1.1:40739999-40740018 None:intergenic 55.0%
CCTCAGATGAGTCGGTCCTT+GGG + chr1.1:40740022-40740041 MS.gene034500:intron 55.0%
GGCTGATAGGATCAGGCAAG+TGG - chr1.1:40742288-40742307 None:intergenic 55.0%
GGTATGTTTAACCCGTGCCC+CGG + chr1.1:40740291-40740310 MS.gene034500:intron 55.0%
GTATGTTTAACCCGTGCCCC+GGG + chr1.1:40740292-40740311 MS.gene034500:intron 55.0%
TAACCTTAACCAGTGCCCCG+GGG - chr1.1:40740170-40740189 None:intergenic 55.0%
TATGTTTAACCCGTGCCCCG+GGG + chr1.1:40740293-40740312 MS.gene034500:intron 55.0%
TGGATGGCCCTTTGTGGATC+AGG + chr1.1:40739774-40739793 MS.gene034500:intron 55.0%
! CAAGGACCGACTCATCTGAG+GGG - chr1.1:40740023-40740042 None:intergenic 55.0%
! CCAAGGACCGACTCATCTGA+GGG - chr1.1:40740024-40740043 None:intergenic 55.0%
! CGTGCTGTGATCGGTTCAAC+TGG + chr1.1:40741245-40741264 MS.gene034500:CDS 55.0%
!! GCTGTTTCTGCTACGGAGGA+TGG + chr1.1:40741101-40741120 MS.gene034500:CDS 55.0%
AAATGCTAACCGGTGCCCCC+GGG + chr1.1:40740151-40740170 MS.gene034500:intron 60.0%
AACCTTAACCAGTGCCCCGG+GGG - chr1.1:40740169-40740188 None:intergenic 60.0%
AGATGAGTCGGTCCTTGGGC+CGG + chr1.1:40740026-40740045 MS.gene034500:intron 60.0%
ATGTTTAACCCGTGCCCCGG+GGG + chr1.1:40740294-40740313 MS.gene034500:intron 60.0%
CCAGCCAGCCTGATCCACAA+AGG - chr1.1:40739785-40739804 None:intergenic 60.0%
CCCTCAGATGAGTCGGTCCT+TGG + chr1.1:40740021-40740040 MS.gene034500:intron 60.0%
CCGCAAGTGAACGGTGGGAT+TGG + chr1.1:40739996-40740015 MS.gene034500:intron 60.0%
CCTTTGTGGATCAGGCTGGC+TGG + chr1.1:40739782-40739801 MS.gene034500:intron 60.0%
GAAATGCTAACCGGTGCCCC+CGG + chr1.1:40740150-40740169 MS.gene034500:intron 60.0%
GATCAGGCAAGTGGAACCGC+GGG - chr1.1:40742279-40742298 None:intergenic 60.0%
GGATCAGGCAAGTGGAACCG+CGG - chr1.1:40742280-40742299 None:intergenic 60.0%
TCTCGCCGCAAGTGAACGGT+GGG + chr1.1:40739991-40740010 MS.gene034500:intron 60.0%
TGGCCCTTTGTGGATCAGGC+TGG + chr1.1:40739778-40739797 MS.gene034500:intron 60.0%
! CCCAAGGACCGACTCATCTG+AGG - chr1.1:40740025-40740044 None:intergenic 60.0%
!! AAAACTCGGTGTCCGGCCCA+AGG - chr1.1:40740041-40740060 None:intergenic 60.0%
AATGCTAACCGGTGCCCCCG+GGG + chr1.1:40740152-40740171 MS.gene034500:intron 65.0%
GGGGTCTCGCCGCAAGTGAA+CGG + chr1.1:40739987-40740006 MS.gene034500:intron 65.0%
GTCTCGCCGCAAGTGAACGG+TGG + chr1.1:40739990-40740009 MS.gene034500:intron 65.0%
GTCCTGCTAACCGGTGCCCC+CGG - chr1.1:40740313-40740332 None:intergenic 70.0%
TCCTGCTAACCGGTGCCCCC+GGG - chr1.1:40740312-40740331 None:intergenic 70.0%
!! TGCCCCCGGGGCACTGGTTA+AGG + chr1.1:40740164-40740183 MS.gene034500:intron 70.0%
CCTGCTAACCGGTGCCCCCG+GGG - chr1.1:40740311-40740330 None:intergenic 75.0%
TAACCGGTGCCCCCGGGGCA+CGG - chr1.1:40740176-40740157 None:intergenic 75.0%
! AACCAGTGCCCCGGGGGCAC+CGG - chr1.1:40740163-40740182 None:intergenic 75.0%
! AACCGGTGCCCCCGGGGCAC+GGG - chr1.1:40740177-40740158 None:intergenic 80.0%
! AACCGGTGCCCCCGGGGCAC+TGG + chr1.1:40740158-40740177 MS.gene034500:intron 80.0%
!! AACCCGTGCCCCGGGGGCAC+CGG + chr1.1:40740300-40740319 MS.gene034500:intron 80.0%
!!! CCCCGGGGGCACCGGTTAGC+AGG + chr1.1:40740308-40740327 MS.gene034500:intron 80.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 40738824 40742828 40738824 ID=MS.gene034500
chr1.1 mRNA 40738824 40742828 40738824 ID=MS.gene034500.t1;Parent=MS.gene034500
chr1.1 exon 40738824 40739136 40738824 ID=MS.gene034500.t1.exon1;Parent=MS.gene034500.t1
chr1.1 CDS 40738824 40739136 40738824 ID=cds.MS.gene034500.t1;Parent=MS.gene034500.t1
chr1.1 exon 40741054 40741418 40741054 ID=MS.gene034500.t1.exon2;Parent=MS.gene034500.t1
chr1.1 CDS 40741054 40741418 40741054 ID=cds.MS.gene034500.t1;Parent=MS.gene034500.t1
chr1.1 exon 40742214 40742450 40742214 ID=MS.gene034500.t1.exon3;Parent=MS.gene034500.t1
chr1.1 CDS 40742214 40742450 40742214 ID=cds.MS.gene034500.t1;Parent=MS.gene034500.t1
chr1.1 exon 40742580 40742828 40742580 ID=MS.gene034500.t1.exon4;Parent=MS.gene034500.t1
chr1.1 CDS 40742580 40742828 40742580 ID=cds.MS.gene034500.t1;Parent=MS.gene034500.t1
Gene Sequence

>MS.gene034500

ATGTTTTCTTGGTTAGCAAGGATAGTTTCAGCGTGCTTGAGACCAGTTCGACGCTATGCACGAATGAACAAAGACAATGAAAATGATTCTGATGAGTCATCAACATCTGAAGATGCTCTTGTTTGGTGTAAAGACCTTGAGAAACACTCTTGTGGTGAATTCTCCTTTGCTATTGTTCAAGCTAATGAAGTAATTGAGGATCATAGTCAAGTTGAAACTGGTTCTGATGCTGTTTTTGTTGGTGTTTATGATGGTCATGGTGGTCCTGAAGCTTCTAAGTTTGTTAATGATCATCTCTTTCATCATCTCATAAGTGAGTCTTAATTTAATTCTTTTATTTATTTTTTCTCTATCACGCTATCTGATCGAAGGCGTGTCCGGTCTCTGACACATGTTTGTTTGACATGACACTGACACATGTGATTTGAATTATATCATTTTTTTGATGTTATTATTTGAGTTGACATATCTGTGTCTCATACATGTCTGGTGTCCGTGTGTCTGTTTCTGTGCTTCATAGGTGTCATAGACATATTGTTTTCTAAGAAAGGCTAGTATGAAGGTTTTTGTGCATTTTTTTAATGTTGAATAAATGGTCTAAAACATCTTAGACAAAGTAGGTTTATGAGTCACGGTTCAATTGCTTTAAACATCTTGAACTGATAAAAAAATTGTGATCAAACCGCCCAATTAATTGGTTGAACAGTTAAATTTGGTTTGATTTTTAAAACATCGCTTTCATGACTAGGACTAATATATATTTTGTTGAAAATTTGGGATTTATTTTTTATTTAATTTATTATAAACAAATTGTTTTGTATTAAATAATATGGTATGGATTTGTTTTGTTGCAATAGATTGAATTGATTTGAATTTGTAGAGTGTTGATCAATGTTTGATATTTTGGACTTTTTTGTTTCATATGTGACTAGCTCTTTTTTGTTGTTATTTGGATGGCCCTTTGTGGATCAGGCTGGCTGGTTGATTTGTTTGGTTTGTTTATATTTCTTACAAGTTAGAACAATCTCAATTATCTTGTAGTTGATTAATGATTATCAATTTAGCTTCTTTTTTTTTTTTTTTTTTTTCTTTTGAGTTTTTCTCTTTCACATCAGGTTCGATTCCCCTTGATGTCCATACAGAGTTATGCTCTGGCTTTAAATGGGGTCTCGCCGCAAGTGAACGGTGGGATTGGACCCCTCAGATGAGTCGGTCCTTGGGCCGGACACCGAGTTTTAAAAAAAAAGAGTTAATATCAACTTATCAATCATGTGTTTTTTTTTATTCTAACAATGAACTTGGTGCATTCTTATAATTAATTTTAGGGAAATGCTAACCGGTGCCCCCGGGGCACTGGTTAAGGTTATGAAAAAGGAAATTACATAGTAGTTAATGCATTGAAATTGTGTAATTAATTTATAATAAAGTCAAAAACAGTTTCCTTTTGTGGTAATAATTCCCTTTTATGGTATGTTTAACCCGTGCCCCGGGGGCACCGGTTAGCAGGACCCTTAATTTTATATCTTATATGAAAGGTTTGCTTGGTTGTGTAGTGGAAAGGGAGAATTTGGGAAATTTGGAAAGAAATTCACTATGCACATCTTTGGCTATGCATAACTACAGGAAATTTTGCTAAACAGCTTAATTAATCTCTTACATAATATCTCATGAATTAAGTTTCTTAAATTCAATAAATTATTTGCATTTTGAAATTTTACTATAATAACACAATTTGGAAATGAATGAGAGCCAAATGGGGGATTGGTAACATCTGCATAAGCTATGACCATAAGTAATTTTCAAAGTTTATTAAGATAAGCTGAATATTTTCTTATAAACATATCATTAATTGTTCAATAAGCTCTTGCAATCATTATTTACACAAGCCTTTGTTTTAGGAGATAAGTTCAAATAATTTTTTCAAACATCACTTTCATTGTGTTCTGTATTTTCACCTTTTAGAATTTGTAACTTGGAAAGCTCGGAATGCGTAAAATATATGATGTTTCCCTTTAAGTTTAGTAATTAGTATGTTAAAAAAATTGACATATCTGTGTTTTTATTTTCTCTTATTCCTATGGATTGCCACATGTTTTTTTATGTTAAGTTACTTTGAATTATTTTTATTGTCTGACCAGCAATGATTACATTTTTAGTAACTTTTAGCTCTATATTTTTCGCAAGTGATCTATAAGAAGTTAAGGAAGTTATGTGTTGAATTTCATTGCAGGGATTGCTAAAGAAAATGGTTCTATCTCCGAAGATATTATAAGGAATGCTGTTTCTGCTACGGAGGATGGATTTCTGACTCTTGTTCGAAGGAGCTTTGGAATAAAGCCGCTGATAGCAGCAATGGGTTCATGTTGTCTTGTTGGTGTTATCTGGAAAGGGACACTTCATATCGCTAATCTTGGAGATTCTCGTGCTGTGATCGGTTCAACTGGTCGATCTAACAAGATCGTTGCTGAACAGTTGACCAAAGAGCATAATGCCTGCAAAGAGGAGATCAGAAGAGAACTCAAGTCTTTGCATCCAGAAGATTCACAAATTGTTGTTATGAAACATGGAACATGGCGCATTAAAGGCATCATCCAGGTTTTCATATTACTTTCTCTTGAGAAATTAATTTCTGTCTATGAACTTTATGTTACCTTTATCGTTTGGTTTTTGCTTCTGATTCCATTGTGTTGTGCCTATACAGTTTTTCTTAAAAAGGTGTTTCAACTTCCATTGTGTTGTGCCTATACAGTTTTTCTTAAAAAGGTGTTTCAACTTCCATTACATGGTTAAATATTTTGTTTTTTTACCTTTTTCTATATACATGAACCATAGTTTGAAGCTGCATTTGATTTCTTTCTTTTCATTTCCTTATTGTATTTGATTTTGTCATGTTTCTTACGATACATGCAAGTCGTCTCTCGATTTGATACAAAACTAAATTCAATCGATACGAATTTCTAGTGCCTATCTATTTTGAACATGAATTTCGTCTTAGGTTTCAATTTATTATGATTCATCCTGATTCACTATGATTCATCCCGATTCATTCTAATGAACCACTACTTAAATGTAGTGTAGCTTGACTTTTTTTATTGTATATCTAGCAACTTTTCTTTTGTCATTTTATTGTATAAATAGATGACATAGTAGTTTGTAATTTTGCCAAGTCACTTATTACTTAAAAATATATATTTTTTTTTTGGTAATATATCTTAAGATTTATAATAAGATTCGCTTCACAATTCGGAAAGTAGGTTTTCTGATTCAAAGTTTGAATCTTGATTTCATAACCATGAATTTAACTAATAATGATGAAATGCATTGTGTGTTGGTTTGTTGTTTTCTCATTGTCACATATGTTTTTCTTCTTCACACCACAACTGGGATCAGGTATCAAAATCCATAGGAGATGCATATTTGAAGCGGCCGGAGTTCTCATTTGATCCTTCATTCCCGCGGTTCCACTTGCCTGATCCTATCAGCCGACCTGTGTTATCAGCAGAACCATCTATGTGTTCAAGAGTTTTACAACCTAACGATAAATTTGTTATATTTGCATCTGATGGCCTTTGGGAACACATGACAAATCAGGAAGCTGCTGAGATCGTTCATAACAACCCTAGAACTGTATGTTTGATGTTTCGTTTAATCATCTTTTTCGTCCACCTAGGATCCTACAATTATGTTTTAGCGTGCGATTCTAATAAAACTGCTCTAGTTTTTCTTAATGTATGCTGTTTATTTCTTTAACATCAGGGGAGTGCAAGAAGACTTCTTCAGGCTGCTTTGAATGAGGCAGCAAGGAAAAGGGAAATGAGATATAAAGATTTGCAGAAGGTTGAAAAGGGAATTCGGCGTTTTTTCCACGATGATATCACAGTGGTTGTTATCTTCATCGACCATGAGATGCAAAAGAAGAATGTTAATGTTCCTGAGTTATCCATCAAAGGGTTTATTGACACAGTTGGACCATCAAAATTTAGAAGTTTCCAAGAAACTCAATGA
Protein sequence

>MS.gene034500.t1

MFSWLARIVSACLRPVRRYARMNKDNENDSDESSTSEDALVWCKDLEKHSCGEFSFAIVQANEVIEDHSQVETGSDAVFVGVYDGHGGPEASKFVNDHLFHHLIRIAKENGSISEDIIRNAVSATEDGFLTLVRRSFGIKPLIAAMGSCCLVGVIWKGTLHIANLGDSRAVIGSTGRSNKIVAEQLTKEHNACKEEIRRELKSLHPEDSQIVVMKHGTWRIKGIIQVSKSIGDAYLKRPEFSFDPSFPRFHLPDPISRPVLSAEPSMCSRVLQPNDKFVIFASDGLWEHMTNQEAAEIVHNNPRTGSARRLLQAALNEAARKREMRYKDLQKVEKGIRRFFHDDITVVVIFIDHEMQKKNVNVPELSIKGFIDTVGPSKFRSFQETQ