AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035020.t1 MTR_1g063020 99.324 296 2 0 1 296 1 296 0.0 616
MS.gene035020.t1 MTR_7g102260 83.162 291 49 0 6 296 3 293 0.0 521
MS.gene035020.t1 MTR_4g057450 69.550 289 88 0 6 294 16 304 2.68e-154 433
MS.gene035020.t1 MTR_2g095800 64.474 304 97 2 2 294 6 309 1.48e-144 409
MS.gene035020.t1 MTR_1g033420 40.604 298 156 7 5 294 7 291 1.24e-69 219
MS.gene035020.t1 MTR_8g064180 37.459 307 175 6 1 294 8 310 1.08e-65 208
MS.gene035020.t1 MTR_1g105900 38.686 274 149 8 32 294 26 291 4.29e-61 196
MS.gene035020.t1 MTR_5g029100 38.603 272 152 4 35 294 37 305 5.01e-61 197
MS.gene035020.t1 MTR_3g089540 41.481 270 140 7 33 294 23 282 3.57e-60 193
MS.gene035020.t1 MTR_7g093530 39.245 265 144 6 39 294 23 279 1.89e-58 189
MS.gene035020.t1 MTR_8g081040 40.076 262 141 6 39 294 30 281 4.86e-58 187
MS.gene035020.t1 MTR_4g128410 38.868 265 145 7 39 294 24 280 1.03e-57 187
MS.gene035020.t1 MTR_5g091730 41.597 238 125 6 61 294 51 278 1.17e-57 187
MS.gene035020.t1 MTR_7g084770 38.545 275 148 8 31 294 23 287 1.72e-57 186
MS.gene035020.t1 MTR_4g128570 40.840 262 130 9 39 294 30 272 4.05e-57 185
MS.gene035020.t1 MTR_6g088320 38.760 258 139 6 48 294 34 283 4.49e-57 185
MS.gene035020.t1 MTR_4g128400 40.310 258 133 7 48 294 36 283 6.38e-57 185
MS.gene035020.t1 MTR_7g084760 38.662 269 144 8 37 294 29 287 8.86e-57 185
MS.gene035020.t1 MTR_3g084630 40.152 264 141 6 37 294 25 277 1.64e-56 184
MS.gene035020.t1 MTR_3g084620 40.152 264 141 6 37 294 25 277 2.01e-56 183
MS.gene035020.t1 MTR_7g084750 38.722 266 142 8 40 294 32 287 9.15e-56 182
MS.gene035020.t1 MTR_4g126920 37.132 272 152 7 34 294 18 281 2.00e-55 181
MS.gene035020.t1 MTR_8g102910 40.840 262 128 8 48 294 30 279 2.11e-55 181
MS.gene035020.t1 MTR_4g045853 38.583 254 133 5 48 286 35 280 5.04e-55 180
MS.gene035020.t1 MTR_6g033085 37.879 264 147 7 40 294 32 287 1.01e-54 179
MS.gene035020.t1 MTR_2g089140 36.000 275 153 5 35 294 20 286 1.43e-54 179
MS.gene035020.t1 MTR_5g029100 39.407 236 130 3 69 294 42 274 1.77e-54 179
MS.gene035020.t1 MTR_4g128580 37.410 278 153 6 30 294 16 285 2.07e-54 178
MS.gene035020.t1 MTR_1g105495 34.437 302 166 9 9 294 9 294 4.68e-54 178
MS.gene035020.t1 MTR_2g038860 36.667 270 132 7 39 286 25 277 7.98e-54 177
MS.gene035020.t1 MTR_2g038705 36.667 270 132 7 39 286 25 277 7.98e-54 177
MS.gene035020.t1 MTR_6g033055 37.500 264 148 7 40 294 32 287 8.36e-54 177
MS.gene035020.t1 MTR_8g102220 37.023 262 136 8 39 294 28 266 4.83e-51 169
MS.gene035020.t1 MTR_8g090100 36.727 275 150 7 35 294 16 281 4.89e-51 171
MS.gene035020.t1 MTR_8g013055 37.736 265 141 8 34 286 25 277 2.61e-50 168
MS.gene035020.t1 MTR_7g056090 37.248 298 150 11 7 294 11 281 7.58e-50 167
MS.gene035020.t1 MTR_4g097640 36.194 268 154 7 36 294 28 287 9.56e-50 166
MS.gene035020.t1 MTR_4g097670 36.194 268 154 7 36 294 28 287 9.56e-50 166
MS.gene035020.t1 MTR_2g089140 37.991 229 119 5 81 294 3 223 2.34e-49 164
MS.gene035020.t1 MTR_4g097700 36.226 265 152 7 39 294 68 324 3.92e-49 166
MS.gene035020.t1 MTR_4g128590 40.455 220 111 6 87 294 64 275 1.09e-48 163
MS.gene035020.t1 MTR_2g049030 32.342 269 156 11 35 294 25 276 6.80e-37 133
MS.gene035020.t1 MTR_2g049130 32.353 272 154 12 37 296 26 279 2.02e-36 132
MS.gene035020.t1 MTR_8g032640 33.333 174 99 6 65 234 38 198 6.65e-15 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035020.t1 AT2G36870 81.443 291 54 0 5 295 8 298 0.0 505
MS.gene035020.t1 AT3G44990 65.085 295 100 2 1 294 1 293 1.03e-139 396
MS.gene035020.t1 AT2G36870 80.117 171 34 0 5 175 8 178 1.02e-100 293
MS.gene035020.t1 AT1G10550 35.714 308 171 6 6 294 10 309 4.53e-65 207
MS.gene035020.t1 AT1G14720 39.405 269 152 5 34 294 25 290 1.59e-64 206
MS.gene035020.t1 AT4G14130 41.791 268 134 7 39 294 27 284 6.13e-64 203
MS.gene035020.t1 AT1G32170 40.511 274 146 6 33 294 25 293 4.46e-62 200
MS.gene035020.t1 AT5G57550 38.246 285 154 6 7 286 6 273 9.05e-62 197
MS.gene035020.t1 AT2G01850 40.152 264 147 5 39 294 30 290 3.54e-61 197
MS.gene035020.t1 AT5G57530 40.370 270 142 8 35 294 22 282 1.31e-60 194
MS.gene035020.t1 AT3G23730 39.852 271 142 7 37 294 24 286 5.17e-60 193
MS.gene035020.t1 AT1G11545 37.500 312 151 10 2 294 13 299 2.15e-58 189
MS.gene035020.t1 AT5G57540 40.000 265 142 8 39 294 25 281 6.10e-58 187
MS.gene035020.t1 AT4G18990 37.193 285 151 6 33 294 33 312 1.53e-57 189
MS.gene035020.t1 AT5G57560 39.483 271 142 9 37 294 20 281 3.14e-57 186
MS.gene035020.t1 AT5G48070 37.377 305 146 10 2 294 10 281 1.31e-56 184
MS.gene035020.t1 AT3G25050 36.466 266 153 5 38 295 33 290 2.30e-56 184
MS.gene035020.t1 AT4G25820 41.729 266 136 9 39 294 29 285 2.79e-56 183
MS.gene035020.t1 AT4G25810 40.000 270 141 9 37 294 23 283 3.15e-56 183
MS.gene035020.t1 AT4G30290 44.681 235 107 7 69 294 56 276 3.16e-56 183
MS.gene035020.t1 AT4G30280 44.681 235 107 7 69 294 61 281 8.81e-56 182
MS.gene035020.t1 AT1G65310 44.589 231 113 7 69 294 61 281 1.95e-55 181
MS.gene035020.t1 AT5G13870 33.887 301 171 7 1 294 8 287 1.97e-55 181
MS.gene035020.t1 AT5G13870 33.887 301 171 7 1 294 8 287 1.97e-55 181
MS.gene035020.t1 AT5G13870 33.887 301 171 7 1 294 8 287 1.97e-55 181
MS.gene035020.t1 AT1G65310 44.589 231 113 7 69 294 83 303 2.79e-55 181
MS.gene035020.t1 AT4G03210 36.194 268 140 5 35 286 24 276 1.33e-54 179
MS.gene035020.t1 AT4G30270 39.098 266 137 8 35 294 19 265 1.44e-54 178
MS.gene035020.t1 AT2G14620 36.093 302 166 10 4 294 9 294 2.56e-54 179
MS.gene035020.t1 AT4G37800 38.806 268 147 6 36 294 30 289 4.80e-54 178
MS.gene035020.t1 AT4G13080 35.880 301 173 8 1 294 4 291 9.87e-54 177
MS.gene035020.t1 AT5G65730 35.235 298 160 8 6 294 17 290 1.09e-53 177
MS.gene035020.t1 AT2G06850 38.267 277 146 9 31 294 26 290 3.24e-53 176
MS.gene035020.t1 AT2G18800 36.803 269 140 8 48 294 36 296 1.94e-52 174
MS.gene035020.t1 AT4G13090 36.333 300 164 7 5 294 6 288 4.58e-52 172
MS.gene035020.t1 AT2G06850 40.336 238 119 8 68 294 5 230 2.78e-50 166
MS.gene035020.t1 AT4G03210 36.800 250 124 6 61 294 25 256 3.44e-49 164
MS.gene035020.t1 AT2G14620 37.872 235 129 7 69 294 18 244 4.90e-49 163
MS.gene035020.t1 AT4G28850 35.581 267 152 5 40 294 29 287 2.93e-48 163
MS.gene035020.t1 AT2G14620 37.991 229 125 7 75 294 13 233 1.43e-47 159
MS.gene035020.t1 AT4G18990 38.208 212 105 5 104 294 32 238 6.35e-42 146
MS.gene035020.t1 AT3G48580 34.082 267 133 10 42 294 36 273 6.67e-40 140
MS.gene035020.t1 AT3G48580 34.034 238 119 8 68 294 4 214 2.19e-36 130
MS.gene035020.t1 AT3G48580 29.963 267 124 11 42 294 36 253 1.94e-24 99.8

Find 91 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGACCAATGTGGGTTTA+TGG 0.178750 1.3:-45496631 MS.gene035020:CDS
CTTCCTTGGGTACCTCCTTA+TGG 0.183924 1.3:+45496672 None:intergenic
GGCCACCTTCCCCTGGTTAC+TGG 0.192690 1.3:-45498579 MS.gene035020:CDS
TGTGGGTTTATGGTTCTATT+TGG 0.204208 1.3:-45496621 MS.gene035020:CDS
ACTATTGTCAAGATCCTAAA+AGG 0.217459 1.3:-45496381 MS.gene035020:CDS
ATCAACATGGAACAACAATT+TGG 0.259205 1.3:-45498480 MS.gene035020:CDS
TTCCTTGGGTACCTCCTTAT+GGG 0.266893 1.3:+45496673 None:intergenic
TTCATCACTATGCTATAGTT+TGG 0.290387 1.3:-45497831 MS.gene035020:CDS
CAATGGTTATAGAAACCTTT+GGG 0.298310 1.3:-45498524 MS.gene035020:CDS
AAGATTTAACCCCAACATTC+AGG 0.298689 1.3:+45496343 None:intergenic
ACATTTGTCTGCAAAGTATA+AGG 0.303238 1.3:+45497934 None:intergenic
CTCCTTCAAGCAATGCCTAT+TGG 0.339681 1.3:-45498600 MS.gene035020:CDS
GAAGTAGACATTGAATTTCT+AGG 0.341397 1.3:-45497973 MS.gene035020:CDS
GTGGGTTTATGGTTCTATTT+GGG 0.354310 1.3:-45496620 MS.gene035020:CDS
CAGACAAATGTTTATATAAG+AGG 0.361231 1.3:-45497922 MS.gene035020:CDS
TAGACTAACACCTGAATGTT+GGG 0.370136 1.3:-45496353 MS.gene035020:CDS
AACAATGTACAGATTCTTAG+TGG 0.388025 1.3:-45496704 MS.gene035020:intron
GAATTTCTAGGGACAACATT+TGG 0.388283 1.3:-45497961 MS.gene035020:CDS
TTCCATGTTGATCCATGCTT+TGG 0.388299 1.3:+45498491 None:intergenic
AATGTTGCATCACTCTTCCT+TGG 0.393842 1.3:+45496658 None:intergenic
ACAATGGTTATAGAAACCTT+TGG 0.410802 1.3:-45498525 MS.gene035020:CDS
AACATTCAGGTGTTAGTCTA+TGG 0.416142 1.3:+45496356 None:intergenic
GTGACTTACATTAATTCCTT+AGG 0.417640 1.3:+45497805 None:intergenic
TTTGTGTACTTTGCAACAAA+AGG 0.422740 1.3:+45496541 None:intergenic
TAAACCCACATTGGTCTTAG+TGG 0.431879 1.3:+45496634 None:intergenic
CCATTTCGATCGGGATACTT+TGG 0.438416 1.3:-45498202 MS.gene035020:CDS
TGCATCACGGTGGTGTCGTC+CGG 0.448578 1.3:-45496491 MS.gene035020:CDS
GCCTATTGGCCACCTTCCCC+TGG 0.450220 1.3:-45498586 MS.gene035020:CDS
ACAACATTGGTCTATGAGTT+GGG 0.459656 1.3:-45496428 MS.gene035020:CDS
CCAAAGTATCCCGATCGAAA+TGG 0.463082 1.3:+45498202 None:intergenic
ACCAGGGGAAGGTGGCCAAT+AGG 0.463800 1.3:+45498585 None:intergenic
AACAACATTGGTCTATGAGT+TGG 0.476903 1.3:-45496429 MS.gene035020:CDS
ATGTTGCATCACTCTTCCTT+GGG 0.478571 1.3:+45496659 None:intergenic
GGCCAATAGGCATTGCTTGA+AGG 0.480007 1.3:+45498598 None:intergenic
TCATCATGGGCAACAGAAGA+TGG 0.483074 1.3:-45496592 MS.gene035020:CDS
TCTAACAATGAAGCGCATCC+AGG 0.493067 1.3:-45498006 MS.gene035020:CDS
TTGGAACTTGGCCAGTAACC+AGG 0.493648 1.3:+45498568 None:intergenic
TCTACTTCATCATGAAATCC+TGG 0.494735 1.3:+45497988 None:intergenic
CGGCCACGGCATCGCCGTAT+CGG 0.495943 1.3:-45496471 MS.gene035020:CDS
CTATTTGGGATGCTTCATCA+TGG 0.504142 1.3:-45496606 MS.gene035020:CDS
ATAGACCTGACTTTGGAACT+TGG 0.504684 1.3:+45498556 None:intergenic
ATTTGGCTTGATAGAACCTC+AGG 0.506453 1.3:-45498463 MS.gene035020:intron
ATAACTCCTGCAGTGTAGCC+AGG 0.507404 1.3:+45498160 None:intergenic
AAGTAGACATTGAATTTCTA+GGG 0.508075 1.3:-45497972 MS.gene035020:CDS
ACGGTGGTGTCGTCCGGCCA+CGG 0.512599 1.3:-45496485 MS.gene035020:CDS
ACTGGCCAAGTTCCAAAGTC+AGG 0.515213 1.3:-45498561 MS.gene035020:CDS
ACGGCATCGCCGTATCGGTC+CGG 0.518193 1.3:-45496466 MS.gene035020:CDS
TATATAAGAGGGAGTGGTGA+TGG 0.520979 1.3:-45497910 MS.gene035020:CDS
CTTCATCCTGGCTACACTGC+AGG 0.522870 1.3:-45498166 MS.gene035020:CDS
TAGTGGATGATATGCCCATA+AGG 0.525035 1.3:-45496687 MS.gene035020:CDS
GCATCGCCGTATCGGTCCGG+TGG 0.538297 1.3:-45496463 MS.gene035020:CDS
GTCGAATCGTCCATTTCGAT+CGG 0.539068 1.3:-45498212 MS.gene035020:CDS
AATGTTTATATAAGAGGGAG+TGG 0.539977 1.3:-45497916 MS.gene035020:CDS
GAGAAATGAAATTTCATCTG+TGG 0.540326 1.3:-45497873 MS.gene035020:CDS
AAAGCTCATAGACCTGACTT+TGG 0.542912 1.3:+45498549 None:intergenic
AGACAAATGTTTATATAAGA+GGG 0.545157 1.3:-45497921 MS.gene035020:CDS
CGATACGGCGATGCCGTGGC+CGG 0.546014 1.3:+45496472 None:intergenic
TGATCCATGCTTTGGTGCTG+AGG 0.547528 1.3:+45498499 None:intergenic
TATTTGGGATGCTTCATCAT+GGG 0.549893 1.3:-45496605 MS.gene035020:CDS
TGCTATAGTTTGGAATCCTA+AGG 0.550228 1.3:-45497821 MS.gene035020:CDS
TCGAATCGTCCATTTCGATC+GGG 0.552144 1.3:-45498211 MS.gene035020:CDS
ATTTCCACTAAGACCAATGT+GGG 0.553604 1.3:-45496638 MS.gene035020:CDS
TGTACTTTGCAACAAAAGGT+TGG 0.556484 1.3:+45496545 None:intergenic
ATAGACTAACACCTGAATGT+TGG 0.559466 1.3:-45496354 MS.gene035020:CDS
GGCCAGTAACCAGGGGAAGG+TGG 0.570410 1.3:+45498577 None:intergenic
GTCAACCCACCGGACCGATA+CGG 0.571686 1.3:+45496457 None:intergenic
ACGACACCACCGTGATGCAT+AGG 0.586001 1.3:+45496495 None:intergenic
TGGAACTTGGCCAGTAACCA+GGG 0.586133 1.3:+45498569 None:intergenic
GGGTCCTCAGCACCAAAGCA+TGG 0.591378 1.3:-45498503 MS.gene035020:CDS
GCTTCGATTAAACTTCATCC+TGG 0.593516 1.3:-45498178 MS.gene035020:CDS
CTATTGTCAAGATCCTAAAA+GGG 0.595880 1.3:-45496380 MS.gene035020:CDS
CATCGCCGTATCGGTCCGGT+GGG 0.599396 1.3:-45496462 MS.gene035020:CDS
CTTGGCCAGTAACCAGGGGA+AGG 0.603822 1.3:+45498574 None:intergenic
TGCCCATAAGGAGGTACCCA+AGG 0.604081 1.3:-45496675 MS.gene035020:CDS
TACACAAACTTCAAAGCTAG+TGG 0.609014 1.3:-45496526 MS.gene035020:CDS
TTGTTGTCTTGTCAACCCAC+CGG 0.610498 1.3:+45496447 None:intergenic
GGACCGATACGGCGATGCCG+TGG 0.614335 1.3:+45496468 None:intergenic
TTTGGTGCTGAGGACCCCAA+AGG 0.620328 1.3:+45498509 None:intergenic
TTGGGTTCAAAGACACCACA+TGG 0.630330 1.3:-45496410 MS.gene035020:CDS
CACCAAAGCATGGATCAACA+TGG 0.631601 1.3:-45498493 MS.gene035020:CDS
CTAGCAAAGAGAAATATGAA+AGG 0.636720 1.3:+45498637 None:intergenic
GGAACTTGGCCAGTAACCAG+GGG 0.650744 1.3:+45498570 None:intergenic
GGTTGACAAGACAACAACAT+TGG 0.653516 1.3:-45496441 MS.gene035020:CDS
AATGGTTATAGAAACCTTTG+GGG 0.656393 1.3:-45498523 MS.gene035020:CDS
ATAGAACCATAAACCCACAT+TGG 0.658123 1.3:+45496625 None:intergenic
CATTTCCACTAAGACCAATG+TGG 0.658778 1.3:-45496639 MS.gene035020:CDS
GTTGCACAGCCTATGCATCA+CGG 0.660505 1.3:-45496504 MS.gene035020:CDS
AGACTAACACCTGAATGTTG+GGG 0.661530 1.3:-45496352 MS.gene035020:CDS
GACAATAGTTATAAACCATG+TGG 0.677923 1.3:+45496395 None:intergenic
TGGATGATATGCCCATAAGG+AGG 0.688479 1.3:-45496684 MS.gene035020:CDS
GCACAGCCTATGCATCACGG+TGG 0.776007 1.3:-45496501 MS.gene035020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAACAATCATA+TGG + chr1.3:45497571-45497590 None:intergenic 10.0%
!! AATAATTTAAATAACAACAA+GGG - chr1.3:45497467-45497486 MS.gene035020:intron 10.0%
!! GAAACTAATTATATATAATA+AGG + chr1.3:45498156-45498175 None:intergenic 10.0%
!! TAATAATTTAAATAACAACA+AGG - chr1.3:45497466-45497485 MS.gene035020:intron 10.0%
!!! TATATTTTTTTCAAAGAAAA+TGG + chr1.3:45497857-45497876 None:intergenic 10.0%
!!! TTTTTTTTATTTCAATCTAA+TGG - chr1.3:45498052-45498071 MS.gene035020:intron 10.0%
!! AAATGAAGTTAATTAATAGA+AGG - chr1.3:45496899-45496918 MS.gene035020:intron 15.0%
!! ATAATTAGAAAGAAAAATGT+AGG + chr1.3:45496746-45496765 None:intergenic 15.0%
!! GATAAATAAATGAAATAGAA+GGG + chr1.3:45497510-45497529 None:intergenic 15.0%
!! TGATAAATAAATGAAATAGA+AGG + chr1.3:45497511-45497530 None:intergenic 15.0%
!!! CATTTTTCTTTCTAATTATT+AGG - chr1.3:45496747-45496766 MS.gene035020:intron 15.0%
!! AAAAATGCAATAATGTAACT+TGG - chr1.3:45497717-45497736 MS.gene035020:intron 20.0%
!! AAAATGCAATAATGTAACTT+GGG - chr1.3:45497718-45497737 MS.gene035020:intron 20.0%
!! AGACAAATGTTTATATAAGA+GGG - chr1.3:45497073-45497092 MS.gene035020:intron 20.0%
!! TTCAAAGAAAATGGTATTAT+TGG + chr1.3:45497848-45497867 None:intergenic 20.0%
!!! AGTTATTTCCAAAATTACAA+TGG - chr1.3:45496650-45496669 MS.gene035020:CDS 20.0%
!!! TGACATTTTGTAAAATGAAA+TGG - chr1.3:45497815-45497834 MS.gene035020:CDS 20.0%
! AAGAAAAAATCACTTTCACA+TGG + chr1.3:45498202-45498221 None:intergenic 25.0%
! AAGTAGACATTGAATTTCTA+GGG - chr1.3:45497022-45497041 MS.gene035020:intron 25.0%
! CAGACAAATGTTTATATAAG+AGG - chr1.3:45497072-45497091 MS.gene035020:intron 25.0%
! GCATATGTTTGTGTTATAAT+CGG - chr1.3:45497596-45497615 MS.gene035020:intron 25.0%
! GGAACCTTACTAATTATAAA+TGG - chr1.3:45498090-45498109 MS.gene035020:intron 25.0%
! GTGACATTTCACTTAATAAA+TGG - chr1.3:45497273-45497292 MS.gene035020:intron 25.0%
! TCAAAAAAGTAGAGCATATA+GGG - chr1.3:45497642-45497661 MS.gene035020:intron 25.0%
! TCTATCTACTGGAAAAATTA+CGG - chr1.3:45496594-45496613 MS.gene035020:CDS 25.0%
! TCTTTCTAATTATTAGGAAG+TGG - chr1.3:45496753-45496772 MS.gene035020:intron 25.0%
! TGACGATTATTATACTATGT+CGG + chr1.3:45497383-45497402 None:intergenic 25.0%
! TTAAAGTTTCAGCAAAATCA+TGG + chr1.3:45496625-45496644 None:intergenic 25.0%
! TTAAATGTGACACTCTTTAA+GGG + chr1.3:45497420-45497439 None:intergenic 25.0%
! TTCAAAAAAGTAGAGCATAT+AGG - chr1.3:45497641-45497660 MS.gene035020:intron 25.0%
! TTCAGCAGAAAAAGAAATAT+TGG + chr1.3:45497671-45497690 None:intergenic 25.0%
! TTTATTATTCCTTTCGAATC+TGG - chr1.3:45497924-45497943 MS.gene035020:CDS 25.0%
!! TTTTCTGCTGAAACATAATA+GGG - chr1.3:45497679-45497698 MS.gene035020:intron 25.0%
!! TTTTTCTGCTGAAACATAAT+AGG - chr1.3:45497678-45497697 MS.gene035020:intron 25.0%
!!! AGCATAGTGATGAAATTTTT+TGG + chr1.3:45497157-45497176 None:intergenic 25.0%
!!! AGTGCCATTTATAATTAGTA+AGG + chr1.3:45498097-45498116 None:intergenic 25.0%
!!! ATTGATTTGTCTGATTCTTA+CGG - chr1.3:45497757-45497776 MS.gene035020:intron 25.0%
!!! TAGTGATGAAATTTTTTGGT+AGG + chr1.3:45497153-45497172 None:intergenic 25.0%
!!! TGTGAAAGTGATTTTTTCTT+TGG - chr1.3:45498202-45498221 MS.gene035020:CDS 25.0%
AACAATGTACAGATTCTTAG+TGG - chr1.3:45498290-45498309 MS.gene035020:intron 30.0%
AATGGTTATAGAAACCTTTG+GGG - chr1.3:45496471-45496490 MS.gene035020:CDS 30.0%
AATGTTTATATAAGAGGGAG+TGG - chr1.3:45497078-45497097 MS.gene035020:intron 30.0%
ACAATGGTTATAGAAACCTT+TGG - chr1.3:45496469-45496488 MS.gene035020:CDS 30.0%
ACATTATATGTGGAGAAGTA+GGG + chr1.3:45497953-45497972 None:intergenic 30.0%
ACATTTGTCTGCAAAGTATA+AGG + chr1.3:45497063-45497082 None:intergenic 30.0%
ACTATTGTCAAGATCCTAAA+AGG - chr1.3:45498613-45498632 MS.gene035020:CDS 30.0%
ATCAACATGGAACAACAATT+TGG - chr1.3:45496514-45496533 MS.gene035020:CDS 30.0%
ATTTCCAAAATTACAATGGC+CGG - chr1.3:45496654-45496673 MS.gene035020:CDS 30.0%
CAATGGTTATAGAAACCTTT+GGG - chr1.3:45496470-45496489 MS.gene035020:CDS 30.0%
CTAATGGTCATGATATTGAA+GGG - chr1.3:45498068-45498087 MS.gene035020:intron 30.0%
CTAGCAAAGAGAAATATGAA+AGG + chr1.3:45496360-45496379 None:intergenic 30.0%
CTATTGTCAAGATCCTAAAA+GGG - chr1.3:45498614-45498633 MS.gene035020:CDS 30.0%
CTTAAATGTGACACTCTTTA+AGG + chr1.3:45497421-45497440 None:intergenic 30.0%
GAAGTAGACATTGAATTTCT+AGG - chr1.3:45497021-45497040 MS.gene035020:intron 30.0%
GACAATAGTTATAAACCATG+TGG + chr1.3:45498602-45498621 None:intergenic 30.0%
GAGAAATGAAATTTCATCTG+TGG - chr1.3:45497121-45497140 MS.gene035020:intron 30.0%
GTGACTTACATTAATTCCTT+AGG + chr1.3:45497192-45497211 None:intergenic 30.0%
TAATGGTCATGATATTGAAG+GGG - chr1.3:45498069-45498088 MS.gene035020:intron 30.0%
TCTAATGGTCATGATATTGA+AGG - chr1.3:45498067-45498086 MS.gene035020:intron 30.0%
TTCATCACTATGCTATAGTT+TGG - chr1.3:45497163-45497182 MS.gene035020:intron 30.0%
TTTGTGTACTTTGCAACAAA+AGG + chr1.3:45498456-45498475 None:intergenic 30.0%
! ATGTCACTTATAGTTGAGAT+CGG + chr1.3:45497260-45497279 None:intergenic 30.0%
!! AGTGGTGATGGAAAAATTAT+AGG - chr1.3:45497096-45497115 MS.gene035020:intron 30.0%
ACATGGCTGCAATTGTATAA+TGG + chr1.3:45498185-45498204 None:intergenic 35.0%
ACTAATCAGATTCATACCTG+AGG + chr1.3:45496550-45496569 None:intergenic 35.0%
AGTGTAGATACTCTATCTAC+TGG - chr1.3:45496583-45496602 MS.gene035020:CDS 35.0%
ATAGAACCATAAACCCACAT+TGG + chr1.3:45498372-45498391 None:intergenic 35.0%
ATAGACTAACACCTGAATGT+TGG - chr1.3:45498640-45498659 MS.gene035020:CDS 35.0%
ATCTACTGGAAAAATTACGG+TGG - chr1.3:45496597-45496616 MS.gene035020:CDS 35.0%
ATTTCCACTAAGACCAATGT+GGG - chr1.3:45498356-45498375 MS.gene035020:intron 35.0%
CACATTATATGTGGAGAAGT+AGG + chr1.3:45497954-45497973 None:intergenic 35.0%
GCTGCAATTGTATAATGGTA+TGG + chr1.3:45498180-45498199 None:intergenic 35.0%
GGATTCACACACATTATATG+TGG + chr1.3:45497963-45497982 None:intergenic 35.0%
GTCTGATTCTTACGGAATAT+AGG - chr1.3:45497765-45497784 MS.gene035020:intron 35.0%
TACACAAACTTCAAAGCTAG+TGG - chr1.3:45498468-45498487 MS.gene035020:CDS 35.0%
TAGACTAACACCTGAATGTT+GGG - chr1.3:45498641-45498660 MS.gene035020:CDS 35.0%
TCTACTTCATCATGAAATCC+TGG + chr1.3:45497009-45497028 None:intergenic 35.0%
TGTACTTTGCAACAAAAGGT+TGG + chr1.3:45498452-45498471 None:intergenic 35.0%
TTCACGAAATCAAATTGTGC+CGG + chr1.3:45496676-45496695 None:intergenic 35.0%
TTTGTGCATGTTTGAATTCG+CGG + chr1.3:45496704-45496723 None:intergenic 35.0%
! AACAACATTGGTCTATGAGT+TGG - chr1.3:45498565-45498584 MS.gene035020:CDS 35.0%
! AACATTCAGGTGTTAGTCTA+TGG + chr1.3:45498641-45498660 None:intergenic 35.0%
! ACAACATTGGTCTATGAGTT+GGG - chr1.3:45498566-45498585 MS.gene035020:CDS 35.0%
! AGGTCTATGAGCTTTTACAA+TGG - chr1.3:45496453-45496472 MS.gene035020:CDS 35.0%
! GAATTTCTAGGGACAACATT+TGG - chr1.3:45497033-45497052 MS.gene035020:intron 35.0%
! GTGGGTTTATGGTTCTATTT+GGG - chr1.3:45498374-45498393 MS.gene035020:intron 35.0%
! TATTTGGGATGCTTCATCAT+GGG - chr1.3:45498389-45498408 MS.gene035020:intron 35.0%
! TGCTATAGTTTGGAATCCTA+AGG - chr1.3:45497173-45497192 MS.gene035020:intron 35.0%
! TGTGGGTTTATGGTTCTATT+TGG - chr1.3:45498373-45498392 MS.gene035020:intron 35.0%
AAAGCTCATAGACCTGACTT+TGG + chr1.3:45496448-45496467 None:intergenic 40.0%
AATGTTGCATCACTCTTCCT+TGG + chr1.3:45498339-45498358 None:intergenic 40.0%
ACTTATAGTTGAGATCGGAG+GGG + chr1.3:45497255-45497274 None:intergenic 40.0%
AGACTAACACCTGAATGTTG+GGG - chr1.3:45498642-45498661 MS.gene035020:CDS 40.0%
ATGTTGCATCACTCTTCCTT+GGG + chr1.3:45498338-45498357 None:intergenic 40.0%
CACTTATAGTTGAGATCGGA+GGG + chr1.3:45497256-45497275 None:intergenic 40.0%
CATTTCCACTAAGACCAATG+TGG - chr1.3:45498355-45498374 MS.gene035020:intron 40.0%
CTAAGACCAATGTGGGTTTA+TGG - chr1.3:45498363-45498382 MS.gene035020:intron 40.0%
GCTTCGATTAAACTTCATCC+TGG - chr1.3:45496816-45496835 MS.gene035020:intron 40.0%
GGTTGACAAGACAACAACAT+TGG - chr1.3:45498553-45498572 MS.gene035020:CDS 40.0%
TATATAAGAGGGAGTGGTGA+TGG - chr1.3:45497084-45497103 MS.gene035020:intron 40.0%
TCACTTATAGTTGAGATCGG+AGG + chr1.3:45497257-45497276 None:intergenic 40.0%
TTCCATGTTGATCCATGCTT+TGG + chr1.3:45496506-45496525 None:intergenic 40.0%
! CTATTTGGGATGCTTCATCA+TGG - chr1.3:45498388-45498407 MS.gene035020:intron 40.0%
! TAGTGGATGATATGCCCATA+AGG - chr1.3:45498307-45498326 MS.gene035020:intron 40.0%
! TTGTCAGCTAGTTTAGTAGC+TGG + chr1.3:45497984-45498003 None:intergenic 40.0%
!! ATAGACCTGACTTTGGAACT+TGG + chr1.3:45496441-45496460 None:intergenic 40.0%
!! ATTTGGCTTGATAGAACCTC+AGG - chr1.3:45496531-45496550 MS.gene035020:CDS 40.0%
!! GTTAGTCTATGGTCCCTTTT+AGG + chr1.3:45498630-45498649 None:intergenic 40.0%
!! TAAACCCACATTGGTCTTAG+TGG + chr1.3:45498363-45498382 None:intergenic 40.0%
CACCAAAGCATGGATCAACA+TGG - chr1.3:45496501-45496520 MS.gene035020:CDS 45.0%
CCATTTCGATCGGGATACTT+TGG - chr1.3:45496792-45496811 MS.gene035020:intron 45.0%
CTCCTTCAAGCAATGCCTAT+TGG - chr1.3:45496394-45496413 MS.gene035020:CDS 45.0%
CTTATAGTTGAGATCGGAGG+GGG + chr1.3:45497254-45497273 None:intergenic 45.0%
GTAGGGAATCCAGATTCGAA+AGG + chr1.3:45497936-45497955 None:intergenic 45.0%
GTCGAATCGTCCATTTCGAT+CGG - chr1.3:45496782-45496801 MS.gene035020:intron 45.0%
TCATCATGGGCAACAGAAGA+TGG - chr1.3:45498402-45498421 MS.gene035020:intron 45.0%
TCGAATCGTCCATTTCGATC+GGG - chr1.3:45496783-45496802 MS.gene035020:intron 45.0%
TCTAACAATGAAGCGCATCC+AGG - chr1.3:45496988-45497007 MS.gene035020:intron 45.0%
TGGATGATATGCCCATAAGG+AGG - chr1.3:45498310-45498329 MS.gene035020:intron 45.0%
TTCCTTGGGTACCTCCTTAT+GGG + chr1.3:45498324-45498343 None:intergenic 45.0%
TTGGGTTCAAAGACACCACA+TGG - chr1.3:45498584-45498603 MS.gene035020:CDS 45.0%
TTGTTGTCTTGTCAACCCAC+CGG + chr1.3:45498550-45498569 None:intergenic 45.0%
! CCAAAGTATCCCGATCGAAA+TGG + chr1.3:45496795-45496814 None:intergenic 45.0%
!! TGTGCCGGCCATTGTAATTT+TGG + chr1.3:45496661-45496680 None:intergenic 45.0%
ACTGGCCAAGTTCCAAAGTC+AGG - chr1.3:45496433-45496452 MS.gene035020:CDS 50.0%
ATAACTCCTGCAGTGTAGCC+AGG + chr1.3:45496837-45496856 None:intergenic 50.0%
CTTCCTTGGGTACCTCCTTA+TGG + chr1.3:45498325-45498344 None:intergenic 50.0%
GTTGCACAGCCTATGCATCA+CGG - chr1.3:45498490-45498509 MS.gene035020:CDS 50.0%
TGGAACTTGGCCAGTAACCA+GGG + chr1.3:45496428-45496447 None:intergenic 50.0%
TTGGAACTTGGCCAGTAACC+AGG + chr1.3:45496429-45496448 None:intergenic 50.0%
! GGCCAATAGGCATTGCTTGA+AGG + chr1.3:45496399-45496418 None:intergenic 50.0%
!! TGATCCATGCTTTGGTGCTG+AGG + chr1.3:45496498-45496517 None:intergenic 50.0%
ACGACACCACCGTGATGCAT+AGG + chr1.3:45498502-45498521 None:intergenic 55.0%
CTTCATCCTGGCTACACTGC+AGG - chr1.3:45496828-45496847 MS.gene035020:intron 55.0%
GGAACTTGGCCAGTAACCAG+GGG + chr1.3:45496427-45496446 None:intergenic 55.0%
TAGTTGAGATCGGAGGGGGT+AGG + chr1.3:45497250-45497269 None:intergenic 55.0%
!! TGCCCATAAGGAGGTACCCA+AGG - chr1.3:45498319-45498338 MS.gene035020:intron 55.0%
!! TTTGGTGCTGAGGACCCCAA+AGG + chr1.3:45496488-45496507 None:intergenic 55.0%
ACCAGGGGAAGGTGGCCAAT+AGG + chr1.3:45496412-45496431 None:intergenic 60.0%
CTTGGCCAGTAACCAGGGGA+AGG + chr1.3:45496423-45496442 None:intergenic 60.0%
GCACAGCCTATGCATCACGG+TGG - chr1.3:45498493-45498512 MS.gene035020:CDS 60.0%
GGAGGGGGTAGGATCGAAAG+AGG + chr1.3:45497239-45497258 None:intergenic 60.0%
GGGTCCTCAGCACCAAAGCA+TGG - chr1.3:45496491-45496510 MS.gene035020:CDS 60.0%
GTCAACCCACCGGACCGATA+CGG + chr1.3:45498540-45498559 None:intergenic 60.0%
!! TGCATCACGGTGGTGTCGTC+CGG - chr1.3:45498503-45498522 MS.gene035020:CDS 60.0%
GCCTATTGGCCACCTTCCCC+TGG - chr1.3:45496408-45496427 MS.gene035020:CDS 65.0%
GGCCACCTTCCCCTGGTTAC+TGG - chr1.3:45496415-45496434 MS.gene035020:CDS 65.0%
GGCCAGTAACCAGGGGAAGG+TGG + chr1.3:45496420-45496439 None:intergenic 65.0%
! ACGGCATCGCCGTATCGGTC+CGG - chr1.3:45498528-45498547 MS.gene035020:CDS 65.0%
! CATCGCCGTATCGGTCCGGT+GGG - chr1.3:45498532-45498551 MS.gene035020:CDS 65.0%
CGATACGGCGATGCCGTGGC+CGG + chr1.3:45498525-45498544 None:intergenic 70.0%
CGGCCACGGCATCGCCGTAT+CGG - chr1.3:45498523-45498542 MS.gene035020:CDS 70.0%
GGACCGATACGGCGATGCCG+TGG + chr1.3:45498529-45498548 None:intergenic 70.0%
! GCATCGCCGTATCGGTCCGG+TGG - chr1.3:45498531-45498550 MS.gene035020:CDS 70.0%
!! ACGGTGGTGTCGTCCGGCCA+CGG - chr1.3:45498509-45498528 MS.gene035020:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 45496348 45498668 45496348 ID=MS.gene035020
chr1.3 mRNA 45496348 45498668 45496348 ID=MS.gene035020.t1;Parent=MS.gene035020
chr1.3 exon 45498464 45498668 45498464 ID=MS.gene035020.t1.exon1;Parent=MS.gene035020.t1
chr1.3 CDS 45498464 45498668 45498464 ID=cds.MS.gene035020.t1;Parent=MS.gene035020.t1
chr1.3 exon 45498147 45498247 45498147 ID=MS.gene035020.t1.exon2;Parent=MS.gene035020.t1
chr1.3 CDS 45498147 45498247 45498147 ID=cds.MS.gene035020.t1;Parent=MS.gene035020.t1
chr1.3 exon 45497814 45498031 45497814 ID=MS.gene035020.t1.exon3;Parent=MS.gene035020.t1
chr1.3 CDS 45497814 45498031 45497814 ID=cds.MS.gene035020.t1;Parent=MS.gene035020.t1
chr1.3 exon 45496348 45496714 45496348 ID=MS.gene035020.t1.exon4;Parent=MS.gene035020.t1
chr1.3 CDS 45496348 45496714 45496348 ID=cds.MS.gene035020.t1;Parent=MS.gene035020.t1
Gene Sequence

>MS.gene035020

ATGGCTTTTCCTTTCATATTTCTCTTTGCTAGTTTATTCTTTATTGCTCCTTCAAGCAATGCCTATTGGCCACCTTCCCCTGGTTACTGGCCAAGTTCCAAAGTCAGGTCTATGAGCTTTTACAATGGTTATAGAAACCTTTGGGGTCCTCAGCACCAAAGCATGGATCAACATGGAACAACAATTTGGCTTGATAGAACCTCAGGTATGAATCTGATTAGTTTCGATTCACGTTAGTGTAGATACTCTATCTACTGGAAAAATTACGGTGGTACCATGATTTTGCTGAAACTTTAAAGTGTAGTTATTTCCAAAATTACAATGGCCGGCACAATTTGATTTCGTGAAACTTACCGCGAATTCAAACATGCACAAAATATATCATTGACATGTAACCTACATTTTTCTTTCTAATTATTAGGAAGTGGATTCAAGTCGAATCGTCCATTTCGATCGGGATACTTTGGCGCTTCGATTAAACTTCATCCTGGCTACACTGCAGGAGTTATAACAGCTTTTTATGTAAGATTAAGATATGATGATGATTAAAAAAATGAAGTTAATTAATAGAAGGTTAAACATTTTTTTAAAAATTTGAGTGTGTGATAAATTTTACTAATGAGTTGATCAATTGCAGCTTTCTAACAATGAAGCGCATCCAGGATTTCATGATGAAGTAGACATTGAATTTCTAGGGACAACATTTGGAAAGCCTTATACTTTGCAGACAAATGTTTATATAAGAGGGAGTGGTGATGGAAAAATTATAGGCAGAGAAATGAAATTTCATCTGTGGTTTGATCCTACCAAAAAATTTCATCACTATGCTATAGTTTGGAATCCTAAGGAATTAATGTAAGTCACTGTTAATTTTCACTCTATATTACTCCTCTTTCGATCCTACCCCCTCCGATCTCAACTATAAGTGACATTTCACTTAATAAATGGTATTGTGTCTAGTTTCTAATATTGATTTTAGATACATAATTTATTAAATGAATTAAAAAAATTAATTTTTTCTTATAATAGAGACCGACATAGTATAATAATCGTCACAGAAAAAATTTATCCCTTAAAGAGTGTCACATTTAAGATATTATTTTTCACTTTTGTATTTATAATAATTTAAATAACAACAAGGGATAGTTTATTAAAATTACCCTTCTATTTCATTTATTTATCATTGTTTTTAATTTGTATGAAATGCTTAAATTAAACAACCATATGATTGTTTTTTTTTTTTAATAAGCATATGTTTGTGTTATAATCGGTTAGACTTTATTTTTGTGCTAGTTCAAAAAAGTAGAGCATATAGGGTAGCCAATATTTCTTTTTCTGCTGAAACATAATAGGGTAGATATGATAACAAAAAAATGCAATAATGTAACTTGGGTCTTTGTCTGTGTCATATTGATTTGTCTGATTCTTACGGAATATAGGTAATACAACAGTGTATGCACGAATGATTGACATTTTGTAAAATGAAATGGAAGCAAACCAATAATACCATTTTCTTTGAAAAAAATATATCATTTTCTTTGAAATTAAAGAATGTGAGAATAGAAGTATAATCTTTTTTATTATTCCTTTCGAATCTGGATTCCCTACTTCTCCACATATAATGTGTGTGAATCCAGCTACTAAACTAGCTGACAAAAAAAAACAGTAGAGAGTTGTCTTACTAAATTTTAATCATAACAATTTTTTTTTTTATTTCAATCTAATGGTCATGATATTGAAGGGGAACCTTACTAATTATAAATGGCACTAATAACAATCTAGTGATCTACATGTGTTTTTTTATACCTTATTATATATAATTAGTTTCACCATACCATTATACAATTGCAGCCATGTGAAAGTGATTTTTTCTTTGGTACAGTGAAGTGATTTTTATTAACTTGTTTTTGTAATATATATTCTTTAACTTACATTATAATTAAACAATGTACAGATTCTTAGTGGATGATATGCCCATAAGGAGGTACCCAAGGAAGAGTGATGCAACATTTCCACTAAGACCAATGTGGGTTTATGGTTCTATTTGGGATGCTTCATCATGGGCAACAGAAGATGGAAAATACAAAGCTGATTACAAATACCAACCTTTTGTTGCAAAGTACACAAACTTCAAAGCTAGTGGTTGCACAGCCTATGCATCACGGTGGTGTCGTCCGGCCACGGCATCGCCGTATCGGTCCGGTGGGTTGACAAGACAACAACATTGGTCTATGAGTTGGGTTCAAAGACACCACATGGTTTATAACTATTGTCAAGATCCTAAAAGGGACCATAGACTAACACCTGAATGTTGGGGTTAA
Protein sequence

>MS.gene035020.t1

MAFPFIFLFASLFFIAPSSNAYWPPSPGYWPSSKVRSMSFYNGYRNLWGPQHQSMDQHGTTIWLDRTSGSGFKSNRPFRSGYFGASIKLHPGYTAGVITAFYLSNNEAHPGFHDEVDIEFLGTTFGKPYTLQTNVYIRGSGDGKIIGREMKFHLWFDPTKKFHHYAIVWNPKELIFLVDDMPIRRYPRKSDATFPLRPMWVYGSIWDASSWATEDGKYKADYKYQPFVAKYTNFKASGCTAYASRWCRPATASPYRSGGLTRQQHWSMSWVQRHHMVYNYCQDPKRDHRLTPECWG