AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035577

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035577.t1 MTR_4g131160 99.658 292 1 0 1 292 109 400 0.0 593
MS.gene035577.t1 MTR_4g131160 98.644 295 1 1 1 292 109 403 0.0 587
MS.gene035577.t1 MTR_1g080890 62.366 186 60 4 73 256 176 353 9.90e-64 204
MS.gene035577.t1 MTR_1g070870 65.854 164 46 3 76 238 108 262 3.24e-56 183
MS.gene035577.t1 MTR_1g070870 65.854 164 46 3 76 238 111 265 4.72e-56 182
MS.gene035577.t1 MTR_3g498825 60.000 85 29 2 88 171 147 227 5.51e-24 99.8
MS.gene035577.t1 MTR_7g053410 51.220 123 49 5 78 195 305 421 1.45e-23 100
MS.gene035577.t1 MTR_7g053410 51.220 123 49 5 78 195 305 421 1.64e-23 100
MS.gene035577.t1 MTR_8g012290 56.522 92 36 2 65 152 265 356 2.19e-23 99.8
MS.gene035577.t1 MTR_8g012290 56.522 92 36 2 65 152 265 356 2.35e-23 99.8
MS.gene035577.t1 MTR_1g017350 60.241 83 32 1 71 152 243 325 2.92e-23 99.0
MS.gene035577.t1 MTR_1g017350 60.241 83 32 1 71 152 243 325 3.17e-23 99.0
MS.gene035577.t1 MTR_1g017350 60.241 83 32 1 71 152 243 325 3.52e-23 98.6
MS.gene035577.t1 MTR_1g017350 60.241 83 32 1 71 152 243 325 3.79e-23 98.6
MS.gene035577.t1 MTR_3g116770 72.727 66 17 1 88 152 191 256 3.89e-23 98.2
MS.gene035577.t1 MTR_3g116770 72.727 66 17 1 88 152 191 256 4.01e-23 98.2
MS.gene035577.t1 MTR_8g065740 64.935 77 26 1 77 152 294 370 1.48e-22 97.4
MS.gene035577.t1 MTR_4g070320 71.212 66 18 1 88 152 143 208 3.35e-22 95.1
MS.gene035577.t1 MTR_5g048860 64.474 76 26 1 78 152 355 430 4.78e-22 96.3
MS.gene035577.t1 MTR_8g099880 57.831 83 30 2 88 169 145 223 6.08e-22 94.0
MS.gene035577.t1 MTR_8g062240 68.182 66 20 1 88 152 151 216 8.80e-22 92.8
MS.gene035577.t1 MTR_5g037250 61.644 73 27 1 81 152 105 177 6.21e-21 89.7
MS.gene035577.t1 MTR_1g084980 55.405 74 29 1 90 163 464 533 5.94e-20 90.1
MS.gene035577.t1 MTR_4g033295 61.538 65 24 1 87 150 117 181 7.02e-20 85.5
MS.gene035577.t1 MTR_1g019240 45.545 101 52 2 56 156 55 152 7.43e-20 87.0
MS.gene035577.t1 MTR_1g018090 48.352 91 44 1 74 164 260 347 1.15e-19 88.2
MS.gene035577.t1 MTR_5g005110 60.000 65 26 0 87 151 223 287 1.25e-19 87.8
MS.gene035577.t1 MTR_4g033295 62.121 66 24 1 87 151 117 182 1.52e-19 84.7
MS.gene035577.t1 MTR_7g099540 57.746 71 26 1 91 161 255 321 1.53e-19 88.6
MS.gene035577.t1 MTR_3g103030 52.632 76 36 0 87 162 228 303 1.81e-19 87.0
MS.gene035577.t1 MTR_1g069155 54.430 79 32 1 83 161 312 386 2.60e-19 88.2
MS.gene035577.t1 MTR_1g052470 60.870 69 26 1 87 154 297 365 3.16e-19 87.0
MS.gene035577.t1 MTR_1g059270 50.000 94 43 3 84 174 156 248 9.34e-19 85.1
MS.gene035577.t1 MTR_1g059270 54.217 83 33 2 84 165 156 234 1.15e-18 83.6
MS.gene035577.t1 MTR_7g099540 52.857 70 33 0 91 160 255 324 2.32e-18 85.1
MS.gene035577.t1 MTR_3g449770 57.143 63 27 0 90 152 311 373 2.83e-18 85.1
MS.gene035577.t1 MTR_7g092510 61.429 70 26 1 84 152 169 238 4.42e-18 83.6
MS.gene035577.t1 MTR_1g106460 43.750 96 50 1 56 151 210 301 4.93e-18 83.2
MS.gene035577.t1 MTR_5g079500 47.674 86 40 2 67 151 200 281 1.06e-17 82.0
MS.gene035577.t1 MTR_1g072530 35.135 148 76 3 94 241 190 317 1.18e-17 82.0
MS.gene035577.t1 MTR_2g098310 59.091 66 26 1 87 151 331 396 1.41e-17 82.4
MS.gene035577.t1 MTR_5g017040 64.815 54 19 0 99 152 116 169 2.22e-17 81.3
MS.gene035577.t1 MTR_1g106470 41.667 96 56 0 67 162 175 270 4.00e-17 80.1
MS.gene035577.t1 MTR_7g111320 52.632 76 33 1 90 165 434 506 4.31e-17 81.6
MS.gene035577.t1 MTR_4g065870 56.522 69 29 1 87 154 354 422 4.74e-17 81.3
MS.gene035577.t1 MTR_8g036235 53.623 69 31 1 87 154 315 383 8.84e-17 80.1
MS.gene035577.t1 MTR_2g098310 58.462 65 26 1 87 150 331 395 1.17e-16 79.7
MS.gene035577.t1 MTR_7g092540 57.812 64 26 1 88 150 243 306 1.91e-16 78.2
MS.gene035577.t1 MTR_7g092540 54.412 68 30 1 88 154 243 310 2.22e-16 78.2
MS.gene035577.t1 MTR_7g110810 55.357 56 25 0 99 154 213 268 3.68e-16 78.2
MS.gene035577.t1 MTR_8g093500 50.667 75 37 0 76 150 102 176 4.88e-16 76.6
MS.gene035577.t1 MTR_4g065870 57.143 63 26 1 87 148 354 416 1.26e-15 77.0
MS.gene035577.t1 MTR_3g028520 54.545 66 29 1 87 151 321 386 1.34e-15 76.6
MS.gene035577.t1 MTR_3g027650 54.545 66 29 1 87 151 321 386 1.34e-15 76.6
MS.gene035577.t1 MTR_3g101810 54.545 66 29 1 95 160 178 242 4.36e-15 73.9
MS.gene035577.t1 MTR_3g027650 56.250 64 27 1 87 149 321 384 6.38e-15 74.7
MS.gene035577.t1 MTR_1g072530 47.222 72 38 0 94 165 190 261 4.36e-14 71.6
MS.gene035577.t1 MTR_1g106480 61.702 47 18 0 106 152 87 133 5.04e-14 68.9
MS.gene035577.t1 MTR_1g116790 53.846 65 29 1 87 150 264 328 7.26e-14 71.2
MS.gene035577.t1 MTR_6g084120 59.016 61 22 2 88 147 155 213 7.49e-14 70.5
MS.gene035577.t1 MTR_7g069590 50.909 55 27 0 95 149 33 87 1.31e-12 64.7
MS.gene035577.t1 MTR_4g097950 43.158 95 44 3 96 184 68 158 2.28e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035577.t1 AT2G24260 62.195 246 63 5 34 263 75 306 2.13e-91 275
MS.gene035577.t1 AT2G24260 72.973 185 41 2 80 263 27 203 4.49e-90 268
MS.gene035577.t1 AT4G30980 59.690 258 69 9 38 291 83 309 3.26e-83 253
MS.gene035577.t1 AT4G30980 59.690 258 69 9 38 291 131 357 3.34e-82 252
MS.gene035577.t1 AT5G58010 57.143 245 79 5 37 267 46 278 7.01e-81 246
MS.gene035577.t1 AT4G02590 62.366 186 59 5 74 255 66 244 1.31e-58 188
MS.gene035577.t1 AT4G02590 62.366 186 59 5 74 255 129 307 6.67e-58 188
MS.gene035577.t1 AT4G02590 62.366 186 59 5 74 255 129 307 6.67e-58 188
MS.gene035577.t1 AT1G03040 65.806 155 38 3 85 238 136 276 3.02e-52 173
MS.gene035577.t1 AT1G03040 65.806 155 38 3 85 238 141 281 4.02e-52 173
MS.gene035577.t1 AT1G03040 59.884 172 51 3 85 238 141 312 4.84e-49 166
MS.gene035577.t1 AT5G50915 58.824 85 30 2 88 171 136 216 1.80e-24 99.8
MS.gene035577.t1 AT5G50915 58.824 85 30 2 88 171 136 216 1.80e-24 99.8
MS.gene035577.t1 AT5G50915 58.824 85 30 2 88 171 136 216 2.28e-24 100
MS.gene035577.t1 AT5G50915 58.824 85 30 2 88 171 136 216 2.28e-24 100
MS.gene035577.t1 AT3G07340 67.123 73 23 1 81 152 251 323 5.13e-23 98.6
MS.gene035577.t1 AT3G07340 67.123 73 23 1 81 152 251 323 6.99e-23 98.2
MS.gene035577.t1 AT4G34530 71.212 66 18 1 88 152 172 237 7.54e-23 96.7
MS.gene035577.t1 AT5G48560 72.727 66 17 1 88 152 301 366 1.06e-22 97.8
MS.gene035577.t1 AT5G48560 72.727 66 17 1 88 152 301 366 1.24e-22 97.8
MS.gene035577.t1 AT1G10120 64.865 74 25 1 80 152 198 271 1.96e-22 95.9
MS.gene035577.t1 AT1G10120 64.865 74 25 1 80 152 198 271 1.96e-22 95.9
MS.gene035577.t1 AT1G68920 71.212 66 18 1 88 152 302 367 2.61e-22 96.7
MS.gene035577.t1 AT1G68920 71.212 66 18 1 88 152 303 368 2.68e-22 96.7
MS.gene035577.t1 AT1G68920 71.212 66 18 1 88 152 303 368 2.68e-22 96.7
MS.gene035577.t1 AT1G68920 71.212 66 18 1 88 152 303 368 2.68e-22 96.7
MS.gene035577.t1 AT1G59640 57.143 84 35 1 70 152 118 201 6.19e-22 94.4
MS.gene035577.t1 AT1G59640 57.143 84 35 1 70 152 118 201 6.68e-22 92.8
MS.gene035577.t1 AT3G23690 69.697 66 19 1 88 152 191 256 1.51e-21 93.6
MS.gene035577.t1 AT2G18300 68.182 66 20 1 88 152 185 250 1.60e-21 93.2
MS.gene035577.t1 AT2G18300 68.182 66 20 1 88 152 185 250 1.66e-21 93.2
MS.gene035577.t1 AT1G26260 59.494 79 27 2 88 165 172 246 2.03e-21 92.8
MS.gene035577.t1 AT2G18300 68.182 66 20 1 88 152 185 250 2.09e-21 93.2
MS.gene035577.t1 AT1G26260 59.494 79 27 2 88 165 201 275 2.41e-21 93.2
MS.gene035577.t1 AT1G26260 59.494 79 27 2 88 165 223 297 2.95e-21 92.8
MS.gene035577.t1 AT1G26260 59.494 79 27 2 88 165 223 297 2.95e-21 92.8
MS.gene035577.t1 AT1G26260 59.494 79 27 2 88 165 223 297 2.95e-21 92.8
MS.gene035577.t1 AT1G26260 59.494 79 27 2 88 165 223 297 2.95e-21 92.8
MS.gene035577.t1 AT4G36540 66.667 66 21 1 88 152 141 206 7.59e-21 90.5
MS.gene035577.t1 AT4G36540 66.667 66 21 1 88 152 141 206 7.72e-21 90.5
MS.gene035577.t1 AT1G73830 54.839 93 34 4 88 179 147 232 1.03e-20 89.4
MS.gene035577.t1 AT1G73830 54.839 93 34 4 88 179 147 232 1.09e-20 89.4
MS.gene035577.t1 AT5G62610 49.474 95 47 1 59 152 124 218 2.72e-20 88.6
MS.gene035577.t1 AT5G62610 49.474 95 47 1 59 152 124 218 2.72e-20 88.6
MS.gene035577.t1 AT2G14760 53.947 76 35 0 87 162 236 311 4.30e-20 89.0
MS.gene035577.t1 AT4G33880 53.947 76 35 0 87 162 265 340 6.29e-20 89.0
MS.gene035577.t1 AT1G25330 58.974 78 30 2 88 164 104 180 7.68e-20 86.3
MS.gene035577.t1 AT1G27740 51.220 82 40 0 87 168 166 247 1.26e-19 86.7
MS.gene035577.t1 AT5G43175 44.340 106 54 2 61 164 103 205 1.57e-19 85.5
MS.gene035577.t1 AT2G42280 47.573 103 48 3 54 154 248 346 3.05e-19 87.0
MS.gene035577.t1 AT1G09530 53.623 69 32 0 85 153 334 402 3.42e-19 87.8
MS.gene035577.t1 AT1G09530 53.623 69 32 0 85 153 334 402 3.42e-19 87.8
MS.gene035577.t1 AT1G09530 53.623 69 32 0 85 153 334 402 3.42e-19 87.8
MS.gene035577.t1 AT1G09530 53.623 69 32 0 85 153 334 402 3.42e-19 87.8
MS.gene035577.t1 AT1G09530 53.623 69 32 0 85 153 334 402 3.42e-19 87.8
MS.gene035577.t1 AT1G09530 53.623 69 32 0 85 153 334 402 3.42e-19 87.8
MS.gene035577.t1 AT2G20180 59.677 62 25 0 91 152 210 271 4.32e-19 87.0
MS.gene035577.t1 AT2G20180 59.677 62 25 0 91 152 210 271 4.32e-19 87.0
MS.gene035577.t1 AT2G20180 59.677 62 25 0 91 152 210 271 4.32e-19 87.0
MS.gene035577.t1 AT1G18400 55.128 78 33 2 88 164 145 221 5.02e-19 84.7
MS.gene035577.t1 AT2G20180 59.677 62 25 0 91 152 281 342 6.66e-19 86.7
MS.gene035577.t1 AT2G20180 59.677 62 25 0 91 152 281 342 6.66e-19 86.7
MS.gene035577.t1 AT2G20180 59.677 62 25 0 91 152 281 342 6.66e-19 86.7
MS.gene035577.t1 AT2G20180 59.677 62 25 0 91 152 281 342 6.66e-19 86.7
MS.gene035577.t1 AT2G42300 62.857 70 25 1 84 152 181 250 6.82e-19 84.7
MS.gene035577.t1 AT1G18400 62.121 66 24 1 88 152 145 210 7.81e-19 84.3
MS.gene035577.t1 AT2G42300 62.857 70 25 1 84 152 181 250 1.08e-18 85.1
MS.gene035577.t1 AT1G66470 44.860 107 49 2 70 166 168 274 2.28e-18 83.6
MS.gene035577.t1 AT3G57800 62.857 70 25 1 84 152 200 269 2.30e-18 84.7
MS.gene035577.t1 AT2G42280 48.980 98 44 3 54 149 248 341 2.90e-18 84.3
MS.gene035577.t1 AT5G37800 43.269 104 55 2 66 168 182 282 3.64e-18 83.2
MS.gene035577.t1 AT2G20180 61.017 59 23 0 91 149 281 339 4.36e-18 83.6
MS.gene035577.t1 AT2G14760 47.191 89 34 1 87 162 236 324 1.02e-17 82.4
MS.gene035577.t1 AT4G09180 57.576 66 27 1 87 151 183 248 1.43e-17 80.9
MS.gene035577.t1 AT1G51140 45.714 105 45 3 51 154 278 371 2.01e-17 82.0
MS.gene035577.t1 AT4G36930 61.017 59 23 0 94 152 197 255 2.90e-17 81.3
MS.gene035577.t1 AT1G35460 57.576 66 27 1 87 151 180 245 2.92e-17 80.1
MS.gene035577.t1 AT4G00050 53.731 67 31 0 85 151 102 168 4.20e-17 80.1
MS.gene035577.t1 AT5G67110 51.471 68 33 0 85 152 84 151 4.91e-17 78.2
MS.gene035577.t1 AT4G00050 53.731 67 31 0 85 151 204 270 8.44e-17 80.1
MS.gene035577.t1 AT4G00050 53.731 67 31 0 85 151 204 270 1.03e-16 80.1
MS.gene035577.t1 AT4G28815 43.210 81 46 0 67 147 47 127 1.50e-15 74.7
MS.gene035577.t1 AT4G28790 50.000 68 34 0 85 152 268 335 1.64e-15 76.6
MS.gene035577.t1 AT4G28815 43.210 81 46 0 67 147 123 203 3.43e-15 74.7
MS.gene035577.t1 AT2G46970 43.902 82 41 2 82 163 187 263 9.04e-15 74.3
MS.gene035577.t1 AT2G46970 43.902 82 41 2 82 163 196 272 9.56e-15 74.3
MS.gene035577.t1 AT2G46970 43.902 82 41 2 82 163 218 294 9.77e-15 74.3
MS.gene035577.t1 AT4G28800 44.000 75 38 1 91 165 252 322 1.42e-14 73.9
MS.gene035577.t1 AT3G59060 58.730 63 26 0 90 152 252 314 1.61e-14 73.9
MS.gene035577.t1 AT3G59060 58.730 63 26 0 90 152 252 314 1.62e-14 73.9
MS.gene035577.t1 AT3G59060 58.730 63 26 0 90 152 252 314 1.62e-14 73.9
MS.gene035577.t1 AT3G59060 58.730 63 26 0 90 152 252 314 1.62e-14 73.9
MS.gene035577.t1 AT2G43010 61.667 60 23 0 91 150 254 313 3.30e-14 72.8
MS.gene035577.t1 AT4G28790 50.000 64 32 0 85 148 268 331 3.43e-14 72.4
MS.gene035577.t1 AT2G43010 61.667 60 23 0 91 150 254 313 3.44e-14 72.8
MS.gene035577.t1 AT2G43010 61.667 60 23 0 91 150 261 320 3.59e-14 72.8
MS.gene035577.t1 AT3G21330 55.000 60 27 0 95 154 276 335 3.87e-14 72.4
MS.gene035577.t1 AT2G43010 61.667 60 23 0 91 150 254 313 4.45e-14 72.4
MS.gene035577.t1 AT2G43010 61.667 60 23 0 91 150 296 355 4.46e-14 72.4
MS.gene035577.t1 AT2G43140 55.385 65 28 1 87 150 230 294 1.15e-13 70.5
MS.gene035577.t1 AT2G43140 55.385 65 28 1 87 150 133 197 1.26e-13 68.9
MS.gene035577.t1 AT4G28800 43.038 79 44 1 91 169 264 341 1.30e-13 71.2
MS.gene035577.t1 AT5G01305 50.000 62 31 0 86 147 35 96 1.67e-13 67.8
MS.gene035577.t1 AT1G05805 47.436 78 36 2 74 150 273 346 3.06e-13 69.7
MS.gene035577.t1 AT4G00120 47.692 65 33 1 97 160 121 185 2.89e-12 64.7
MS.gene035577.t1 AT3G57800 39.316 117 23 2 84 152 200 316 3.00e-12 67.0
MS.gene035577.t1 AT2G42300 69.767 43 13 0 84 126 181 223 8.65e-12 63.9
MS.gene035577.t1 AT2G42300 69.767 43 13 0 84 126 181 223 1.07e-11 63.5
MS.gene035577.t1 AT2G42300 69.767 43 13 0 84 126 181 223 1.07e-11 63.5
MS.gene035577.t1 AT2G46510 38.298 94 49 2 65 156 367 453 3.42e-11 63.9
MS.gene035577.t1 AT2G20100 31.429 140 77 5 104 238 219 344 6.85e-11 62.8

Find 100 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTGTTAACGACGTCGTTC+TGG 0.234818 8.4:-2197830 None:intergenic
CCCCTCCGTTGCCGTTTGTT+TGG 0.284955 8.4:-2200768 None:intergenic
TGAGAGGATGAAAGCTTTGC+AGG 0.309874 8.4:+2198600 MS.gene035577:CDS
CATTGTGAGGCTTTCGTTAG+AGG 0.309986 8.4:-2200821 None:intergenic
ATGCTTCTCACCGCCGATTC+CGG 0.331027 8.4:+2197771 MS.gene035577:CDS
ATAGCAGTAGCAAGAGAAAT+AGG 0.336283 8.4:-2200928 None:intergenic
GAACGATAGGGGCAGAGTTT+TGG 0.337044 8.4:+2198137 MS.gene035577:intron
TCACCGTTAGATGCGGTGAT+AGG 0.343503 8.4:-2201024 None:intergenic
CTCAGCCAAGGCAGAAGGTT+AGG 0.345610 8.4:+2198210 MS.gene035577:CDS
AGTGTTACCTTGTTTGCATT+AGG 0.362556 8.4:-2198631 None:intergenic
GATGCTGTTAACGGACATTC+CGG 0.365589 8.4:-2201061 None:intergenic
ATCTTTGACGATGCTGTTAA+CGG 0.380837 8.4:-2201070 None:intergenic
GAGCTCCACTCGCCGGAGCT+TGG 0.406538 8.4:-2198184 None:intergenic
ACCTTCTGCCTTGGCTGAGC+CGG 0.408333 8.4:-2198206 None:intergenic
CATGTCTTCTTCCATTAGCT+TGG 0.408536 8.4:-2200860 None:intergenic
AGCGTTGATCAAAGGGTTCC+TGG 0.409141 8.4:-2200971 None:intergenic
GCATCAAGTGGCCAAGCTAA+TGG 0.427063 8.4:+2200849 MS.gene035577:CDS
CCCTTTCGGCGATGCTGTGT+GGG 0.435060 8.4:-2198256 None:intergenic
GCTCTCTTCAACGGTTTCTC+CGG 0.441559 8.4:+2197976 MS.gene035577:CDS
TAGTGAGATACATACCCTTT+CGG 0.447375 8.4:-2198270 None:intergenic
GTGGAGGAGACCTCCGGAAT+CGG 0.449433 8.4:-2197784 None:intergenic
CTTGCCCTAACCTTCTGCCT+TGG 0.449584 8.4:-2198215 None:intergenic
ATGCTGTTAACGGACATTCC+GGG 0.457739 8.4:-2201060 None:intergenic
GACAGACAAGGCTTCCATGC+TGG 0.473916 8.4:+2199890 MS.gene035577:intron
TCAGCCAAGGCAGAAGGTTA+GGG 0.474534 8.4:+2198211 MS.gene035577:CDS
GGTTGAAAATGATGAGGTTG+AGG 0.476792 8.4:-2198015 None:intergenic
GGTGTTAACGACGTCGTTCT+GGG 0.478074 8.4:-2197829 None:intergenic
TATTAGCAGCGTTGATCAAA+GGG 0.485300 8.4:-2200978 None:intergenic
AGGCATGTGGAGGAGACCTC+CGG 0.490529 8.4:-2197790 None:intergenic
CACCGTTAGATGCGGTGATA+GGG 0.491206 8.4:-2201023 None:intergenic
TCCGTTCAAGCTCTCTTCAA+CGG 0.500853 8.4:+2197967 MS.gene035577:CDS
CCCACACAGCATCGCCGAAA+GGG 0.501568 8.4:+2198256 MS.gene035577:CDS
ACCCTTTCGGCGATGCTGTG+TGG 0.505234 8.4:-2198257 None:intergenic
AGCTTGGTTCGTTGCACTCA+CGG 0.516430 8.4:-2198168 None:intergenic
AGCTCCGGCGAGTGGAGCTC+CGG 0.517913 8.4:+2198187 MS.gene035577:CDS
TTATTAGCAGCGTTGATCAA+AGG 0.525147 8.4:-2200979 None:intergenic
AGTGACTGCGTCCAAACAAA+CGG 0.526034 8.4:+2200757 MS.gene035577:CDS
AGGGGAATCTCACTTTGACT+TGG 0.526596 8.4:-2199947 None:intergenic
CGATTATGTGAAGTTCCTAC+AGG 0.526815 8.4:+2199923 MS.gene035577:CDS
AGGGACACCATGGAGAGATC+CGG 0.526947 8.4:-2197995 None:intergenic
CAAGCTAATGGAAGAAGACA+TGG 0.531731 8.4:+2200861 MS.gene035577:CDS
GGTGATGGGCCATCCTCTCC+CGG 0.535124 8.4:+2201042 MS.gene035577:CDS
GAGTGCAACGAACCAAGCTC+CGG 0.538396 8.4:+2198172 MS.gene035577:CDS
ACCGTTGAAGAGAGCTTGAA+CGG 0.539080 8.4:-2197968 None:intergenic
GTGGAGCTCCGGCTCAGCCA+AGG 0.540877 8.4:+2198198 MS.gene035577:CDS
TGAGCCGGAGCTCCACTCGC+CGG 0.543105 8.4:-2198191 None:intergenic
TCCAAACAAACGGCAACGGA+GGG 0.544554 8.4:+2200767 MS.gene035577:CDS
GTTAACGGACATTCCGGGAG+AGG 0.549252 8.4:-2201055 None:intergenic
ACGAACCAAGCTCCGGCGAG+TGG 0.549919 8.4:+2198179 MS.gene035577:CDS
TGGGAGGAGTCAAAGTCAGC+AGG 0.551562 8.4:-2197810 None:intergenic
GTTGAAAATGATGAGGTTGA+GGG 0.552496 8.4:-2198014 None:intergenic
AGGGGCTGTAGCTCCTCTTG+TGG 0.552998 8.4:+2200208 MS.gene035577:CDS
ATGCAATACTTGCAAGGCAA+AGG 0.553212 8.4:+2200892 MS.gene035577:CDS
ACGGACATTCCGGGAGAGGA+TGG 0.558510 8.4:-2201051 None:intergenic
AACCCTATCACCGCATCTAA+CGG 0.559814 8.4:+2201021 MS.gene035577:CDS
GGTTTCTCCGGATCTCTCCA+TGG 0.559827 8.4:+2197988 MS.gene035577:CDS
ATCACCGCATCTAACGGTGA+TGG 0.562234 8.4:+2201027 MS.gene035577:CDS
TCACCGCATCTAACGGTGAT+GGG 0.564709 8.4:+2201028 MS.gene035577:CDS
CTTGATGCTCCGTCATTGTG+AGG 0.566568 8.4:-2200834 None:intergenic
CAACTGCCACGTGTCACACC+AGG 0.567988 8.4:+2200953 MS.gene035577:CDS
ACCCACACAGCATCGCCGAA+AGG 0.569008 8.4:+2198255 MS.gene035577:CDS
TATGAATATACTCACGGAGG+AGG 0.570799 8.4:-2197850 None:intergenic
GGCGATGCTGTGTGGGTCGG+TGG 0.575489 8.4:-2198249 None:intergenic
CAAGGCAGAAGGTTAGGGCA+AGG 0.577404 8.4:+2198216 MS.gene035577:CDS
TCACTTTGACTTGGACCTGT+AGG 0.579189 8.4:-2199938 None:intergenic
CAAAGTCAGCAGGCATGTGG+AGG 0.582463 8.4:-2197800 None:intergenic
TCCGGCTCAGCCAAGGCAGA+AGG 0.583403 8.4:+2198205 MS.gene035577:CDS
ATCGATGATCTCATCCAGCA+TGG 0.593330 8.4:-2199904 None:intergenic
AGTCAAAGTCAGCAGGCATG+TGG 0.593631 8.4:-2197803 None:intergenic
TTTGAGTATGAGCAGATTAG+GGG 0.596866 8.4:+2200181 MS.gene035577:CDS
TTCGGCGATGCTGTGTGGGT+CGG 0.599244 8.4:-2198252 None:intergenic
GTCCAAACAAACGGCAACGG+AGG 0.599582 8.4:+2200766 MS.gene035577:CDS
TTTGCCTTGCAAGTATTGCA+TGG 0.601415 8.4:-2200890 None:intergenic
TTATGTATGGCAGTTACGGA+GGG 0.606270 8.4:+2198567 MS.gene035577:intron
TGCGTCCAAACAAACGGCAA+CGG 0.608283 8.4:+2200763 MS.gene035577:CDS
TTTATGTATGGCAGTTACGG+AGG 0.609201 8.4:+2198566 MS.gene035577:intron
CACAATGACGGAGCATCAAG+TGG 0.616267 8.4:+2200837 MS.gene035577:CDS
GATGAGGTTGAGGGACACCA+TGG 0.619769 8.4:-2198005 None:intergenic
ACTTGTTCCTAATGCAAACA+AGG 0.620351 8.4:+2198624 MS.gene035577:CDS
GAGGAGACCTCCGGAATCGG+CGG 0.620378 8.4:-2197781 None:intergenic
TAACGAAAGCCTCACAATGA+CGG 0.625198 8.4:+2200825 MS.gene035577:CDS
CTTCTCACCGCCGATTCCGG+AGG 0.626580 8.4:+2197774 MS.gene035577:CDS
TTGAGTATGAGCAGATTAGG+GGG 0.628679 8.4:+2200182 MS.gene035577:CDS
GCTGCTAATAACAATATCAA+CGG 0.630462 8.4:+2200991 MS.gene035577:CDS
TCTGCCATGCAATACTTGCA+AGG 0.632578 8.4:+2200886 MS.gene035577:CDS
AGAAAGAATAGAACCTGAGG+AGG 0.639672 8.4:-2198036 None:intergenic
CCAAACAAACGGCAACGGAG+GGG 0.643439 8.4:+2200768 MS.gene035577:CDS
CAGAAGGTTAGGGCAAGGAG+AGG 0.644091 8.4:+2198221 MS.gene035577:CDS
AGGGTTCCTGGTGTGACACG+TGG 0.654023 8.4:-2200959 None:intergenic
TGGCCCATCACCGTTAGATG+CGG 0.659070 8.4:-2201031 None:intergenic
GAGGACATATCAGCCACAAG+AGG 0.664253 8.4:-2200221 None:intergenic
TAGCAAGAGAAATAGGCATG+AGG 0.667127 8.4:-2200921 None:intergenic
GTTAACGACGTCGTTCTGGG+AGG 0.675970 8.4:-2197826 None:intergenic
TGTGGATCGACAGACAGACA+AGG 0.682348 8.4:+2199878 MS.gene035577:intron
AAGTGGAAAATTTACCTCGG+AGG 0.684381 8.4:-2200240 None:intergenic
AAGCTAATGGAAGAAGACAT+GGG 0.700225 8.4:+2200862 MS.gene035577:CDS
CGGCGTCTACGACTTAGAAG+CGG 0.702696 8.4:-2201100 None:intergenic
TGTGGCTGATATGTCCTCCG+AGG 0.708103 8.4:+2200226 MS.gene035577:CDS
TGAGGAGGTTGAAAATGATG+AGG 0.716913 8.4:-2198021 None:intergenic
GGAGGGAAAGAATTGCTGAG+AGG 0.735960 8.4:+2198584 MS.gene035577:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTAAAATTTCAACA+TGG - chr8.4:2199850-2199869 None:intergenic 10.0%
!! ACTATAAATAATAATAACTT+TGG - chr8.4:2200280-2200299 None:intergenic 10.0%
!! AGATAAAAATAAATTGTAAT+AGG - chr8.4:2200439-2200458 None:intergenic 10.0%
!! ATTAAAGAAAAATAATACTT+TGG + chr8.4:2198968-2198987 MS.gene035577:intron 10.0%
!!! ATAGTTTTCTTTTTTTAATA+AGG - chr8.4:2199461-2199480 None:intergenic 10.0%
!!! ATTTGATTTATTTCTTTTTT+GGG + chr8.4:2198109-2198128 MS.gene035577:intron 10.0%
!!! TGAAATTTTAATTATTTGTT+TGG + chr8.4:2199853-2199872 MS.gene035577:intron 10.0%
!! ACTTATAAACATAATGTAAT+GGG + chr8.4:2199815-2199834 MS.gene035577:intron 15.0%
!! CTTAATTTAATACTCAATTA+TGG + chr8.4:2198657-2198676 MS.gene035577:intron 15.0%
!! TAACGTTGTTAATTAATTAA+CGG - chr8.4:2197886-2197905 None:intergenic 15.0%
!! TGTAATTTAGATTAAAATTG+TGG - chr8.4:2199222-2199241 None:intergenic 15.0%
!!! ATTTTTTATTCCATTGAATT+AGG - chr8.4:2200407-2200426 None:intergenic 15.0%
!!! GATTTGATTTATTTCTTTTT+TGG + chr8.4:2198108-2198127 MS.gene035577:intron 15.0%
!!! TTTTTCTTCAATATACTTTT+GGG + chr8.4:2199971-2199990 MS.gene035577:intron 15.0%
!! AAACAAACTAATAGTTGATT+AGG - chr8.4:2198945-2198964 None:intergenic 20.0%
!! AAAGTATATTGAAGAAAAAG+AGG - chr8.4:2199970-2199989 None:intergenic 20.0%
!! AAGCTAACAAAAAATAAAGT+TGG - chr8.4:2200594-2200613 None:intergenic 20.0%
!! AAGTATATTGAAGAAAAAGA+GGG - chr8.4:2199969-2199988 None:intergenic 20.0%
!! AATTAATCACATGAGAAAAT+TGG - chr8.4:2198784-2198803 None:intergenic 20.0%
!! AATTATGCTCAAAAAATTGT+CGG - chr8.4:2199357-2199376 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG + chr8.4:2199032-2199051 MS.gene035577:intron 20.0%
!! AGAAAATATGAATATACTCA+CGG - chr8.4:2197859-2197878 None:intergenic 20.0%
!! CACTTATAAACATAATGTAA+TGG + chr8.4:2199814-2199833 MS.gene035577:intron 20.0%
!!! AGTAGTAAACTTTTTTTTGA+GGG - chr8.4:2198826-2198845 None:intergenic 20.0%
!!! ATCTGATATCTTTTTTTGTA+AGG + chr8.4:2199538-2199557 MS.gene035577:intron 20.0%
!!! CTTTTTCTTCAATATACTTT+TGG + chr8.4:2199970-2199989 MS.gene035577:intron 20.0%
!!! GATTTTAATACACTGTTTTT+TGG - chr8.4:2199292-2199311 None:intergenic 20.0%
!!! GGTAATTTTGTTTTTATGTA+TGG + chr8.4:2198554-2198573 MS.gene035577:intron 20.0%
!!! TGAGTATTTATTTCTTCTTA+TGG - chr8.4:2200526-2200545 None:intergenic 20.0%
!!! TTTCTTTTTTGAAAGATCAA+TGG - chr8.4:2200337-2200356 None:intergenic 20.0%
! ACAGAATATAAACCCTTATT+TGG + chr8.4:2198431-2198450 MS.gene035577:intron 25.0%
! ACATCTTGAGAAAATTATTG+AGG - chr8.4:2199754-2199773 None:intergenic 25.0%
! AGTATATTGAAGAAAAAGAG+GGG - chr8.4:2199968-2199987 None:intergenic 25.0%
! ATTATGCTCAAAAAATTGTC+GGG - chr8.4:2199356-2199375 None:intergenic 25.0%
! CTACAAATTGAAAATATCAC+AGG - chr8.4:2199408-2199427 None:intergenic 25.0%
! GTAACATAGGATTAATTGAA+TGG + chr8.4:2198998-2199017 MS.gene035577:intron 25.0%
! GTAATAGTACAATGGTAATT+TGG + chr8.4:2198533-2198552 MS.gene035577:intron 25.0%
! TCATTGTATGAAAATATCAG+AGG + chr8.4:2199511-2199530 MS.gene035577:intron 25.0%
! TCTCAAGTTAACTTATTTCA+AGG + chr8.4:2200105-2200124 MS.gene035577:intron 25.0%
! TTAATTATTTGTTTGGTCTG+TGG + chr8.4:2199860-2199879 MS.gene035577:intron 25.0%
! TTTCTTGCTTTCTCAATTTA+TGG - chr8.4:2200469-2200488 None:intergenic 25.0%
!! AAAAAAAGAAACTTTTCTCG+TGG + chr8.4:2200300-2200319 MS.gene035577:intron 25.0%
!! CAGAATATAAACCCTTATTT+GGG + chr8.4:2198432-2198451 MS.gene035577:intron 25.0%
!! TCAATAATTTTCTCAAGATG+TGG + chr8.4:2199753-2199772 MS.gene035577:intron 25.0%
!! TTTTCTTAATTGACTGAATG+CGG + chr8.4:2199607-2199626 MS.gene035577:intron 25.0%
!! TTTTTGTAAGGAAGTCAATT+GGG + chr8.4:2199550-2199569 MS.gene035577:intron 25.0%
!!! CAATTTTTTCAGAGAAAATG+CGG + chr8.4:2198391-2198410 MS.gene035577:intron 25.0%
!!! GAGTAGTAAACTTTTTTTTG+AGG - chr8.4:2198827-2198846 None:intergenic 25.0%
!!! GTTTTTTTTTGACACAAATC+TGG + chr8.4:2199145-2199164 MS.gene035577:intron 25.0%
!!! TTAATATTGTTAGCTTTTGC+AGG + chr8.4:2197930-2197949 MS.gene035577:intron 25.0%
!!! TTTTTTGTAAGGAAGTCAAT+TGG + chr8.4:2199549-2199568 MS.gene035577:intron 25.0%
AAATATGAATATACTCACGG+AGG - chr8.4:2197856-2197875 None:intergenic 30.0%
AACAGAAACAGAATGATAGT+AGG - chr8.4:2198468-2198487 None:intergenic 30.0%
AGAAAAAGCAGAAGAAAAGT+GGG - chr8.4:2200369-2200388 None:intergenic 30.0%
AGAAAGTGGAAAATTTACCT+CGG - chr8.4:2200246-2200265 None:intergenic 30.0%
ATTGAGAAAGCAAGAAATTG+AGG + chr8.4:2200472-2200491 MS.gene035577:intron 30.0%
GCTGCTAATAACAATATCAA+CGG + chr8.4:2200991-2201010 MS.gene035577:CDS 30.0%
TGCTTTCTCAATTTATGGTA+GGG - chr8.4:2200464-2200483 None:intergenic 30.0%
TGGTAAATTCAGAAAGAAAG+TGG - chr8.4:2200260-2200279 None:intergenic 30.0%
TTATGCTCAAAAAATTGTCG+GGG - chr8.4:2199355-2199374 None:intergenic 30.0%
TTGCTTTCTCAATTTATGGT+AGG - chr8.4:2200465-2200484 None:intergenic 30.0%
! AATATAAACCCTTATTTGGG+AGG + chr8.4:2198435-2198454 MS.gene035577:intron 30.0%
! ATATAAACCCTTATTTGGGA+GGG + chr8.4:2198436-2198455 MS.gene035577:intron 30.0%
! CTTGTTTCTTCCTAATTCAA+TGG + chr8.4:2200394-2200413 MS.gene035577:intron 30.0%
! GTTTTTATGTATGGCAGTTA+CGG + chr8.4:2198563-2198582 MS.gene035577:intron 30.0%
! TCTGAAAAAATTGCACGTTT+TGG - chr8.4:2198384-2198403 None:intergenic 30.0%
!! CTCTTTCTTTTGTTTCCTTT+TGG + chr8.4:2198496-2198515 MS.gene035577:intron 30.0%
!! TTTTGAGTATGAGCAGATTA+GGG + chr8.4:2200180-2200199 MS.gene035577:CDS 30.0%
!! TTTTGGGAATTTGAACGATA+GGG + chr8.4:2198125-2198144 MS.gene035577:intron 30.0%
!! TTTTTGGGAATTTGAACGAT+AGG + chr8.4:2198124-2198143 MS.gene035577:intron 30.0%
AAGCTAATGGAAGAAGACAT+GGG + chr8.4:2200862-2200881 MS.gene035577:CDS 35.0%
ACTTGTTCCTAATGCAAACA+AGG + chr8.4:2198624-2198643 MS.gene035577:CDS 35.0%
AGTTGTGGTTAATTCAATCG+AGG - chr8.4:2199255-2199274 None:intergenic 35.0%
ATAGCAGTAGCAAGAGAAAT+AGG - chr8.4:2200931-2200950 None:intergenic 35.0%
ATCTTTGACGATGCTGTTAA+CGG - chr8.4:2201073-2201092 None:intergenic 35.0%
CAGAAAAAGCAGAAGAAAAG+TGG - chr8.4:2200370-2200389 None:intergenic 35.0%
CTAAGAAAGAATAGAACCTG+AGG - chr8.4:2198042-2198061 None:intergenic 35.0%
GTATCTCACTATAACGTGTT+TGG + chr8.4:2198282-2198301 MS.gene035577:intron 35.0%
GTTGAAAATGATGAGGTTGA+GGG - chr8.4:2198017-2198036 None:intergenic 35.0%
GTTTGGTTGTGCAGAAAATT+GGG + chr8.4:2198299-2198318 MS.gene035577:intron 35.0%
TAGTGAGATACATACCCTTT+CGG - chr8.4:2198273-2198292 None:intergenic 35.0%
TGTAAATCCAATTACGCAAC+AGG - chr8.4:2200158-2200177 None:intergenic 35.0%
TGTTGCGTAATTGGATTTAC+AGG + chr8.4:2200157-2200176 MS.gene035577:intron 35.0%
TGTTTGGTTGTGCAGAAAAT+TGG + chr8.4:2198298-2198317 MS.gene035577:intron 35.0%
TTCCTTAATCTGTGTGCAAT+TGG - chr8.4:2198879-2198898 None:intergenic 35.0%
TTTGAGTATGAGCAGATTAG+GGG + chr8.4:2200181-2200200 MS.gene035577:CDS 35.0%
TTTGGGAATTTGAACGATAG+GGG + chr8.4:2198126-2198145 MS.gene035577:intron 35.0%
TTTGTTACCTGTTGCGTAAT+TGG + chr8.4:2200148-2200167 MS.gene035577:intron 35.0%
! AGTGTTACCTTGTTTGCATT+AGG - chr8.4:2198634-2198653 None:intergenic 35.0%
! CTTTGGAAGTAGTGTAACAT+AGG + chr8.4:2198985-2199004 MS.gene035577:intron 35.0%
! GTACATGATGTTTAGAGTTG+TGG - chr8.4:2199270-2199289 None:intergenic 35.0%
! TGTTTCCTTTTGGATCAATG+TGG + chr8.4:2198506-2198525 MS.gene035577:intron 35.0%
!! GTTTTGAGTATGAGCAGATT+AGG + chr8.4:2200179-2200198 MS.gene035577:CDS 35.0%
!! TATTAGCAGCGTTGATCAAA+GGG - chr8.4:2200981-2201000 None:intergenic 35.0%
!! TTATTAGCAGCGTTGATCAA+AGG - chr8.4:2200982-2201001 None:intergenic 35.0%
AAAATGCGGTTGTTTGTCAG+TGG + chr8.4:2198405-2198424 MS.gene035577:intron 40.0%
AACAGAATGATAGTAGGCGT+AGG - chr8.4:2198462-2198481 None:intergenic 40.0%
AAGTGGAAAATTTACCTCGG+AGG - chr8.4:2200243-2200262 None:intergenic 40.0%
AGAAAGAATAGAACCTGAGG+AGG - chr8.4:2198039-2198058 None:intergenic 40.0%
AGCCAATTGCACACAGATTA+AGG + chr8.4:2198874-2198893 MS.gene035577:intron 40.0%
ATGCAATACTTGCAAGGCAA+AGG + chr8.4:2200892-2200911 MS.gene035577:CDS 40.0%
CAAGCTAATGGAAGAAGACA+TGG + chr8.4:2200861-2200880 MS.gene035577:CDS 40.0%
CATGTCTTCTTCCATTAGCT+TGG - chr8.4:2200863-2200882 None:intergenic 40.0%
CGATTATGTGAAGTTCCTAC+AGG + chr8.4:2199923-2199942 MS.gene035577:CDS 40.0%
GCTCAATCAGCTAAAACAAG+TGG - chr8.4:2199705-2199724 None:intergenic 40.0%
GGTTGAAAATGATGAGGTTG+AGG - chr8.4:2198018-2198037 None:intergenic 40.0%
GTGGAAGGGTAATAGTACAA+TGG + chr8.4:2198525-2198544 MS.gene035577:intron 40.0%
TAACGAAAGCCTCACAATGA+CGG + chr8.4:2200825-2200844 MS.gene035577:CDS 40.0%
TAGCAAGAGAAATAGGCATG+AGG - chr8.4:2200924-2200943 None:intergenic 40.0%
TATGAATATACTCACGGAGG+AGG - chr8.4:2197853-2197872 None:intergenic 40.0%
TGAGGAGGTTGAAAATGATG+AGG - chr8.4:2198024-2198043 None:intergenic 40.0%
TGTAAGGAAGTCAATTGGGT+CGG + chr8.4:2199554-2199573 MS.gene035577:intron 40.0%
TTATGTATGGCAGTTACGGA+GGG + chr8.4:2198567-2198586 MS.gene035577:intron 40.0%
TTGAGTATGAGCAGATTAGG+GGG + chr8.4:2200182-2200201 MS.gene035577:CDS 40.0%
TTTATGTATGGCAGTTACGG+AGG + chr8.4:2198566-2198585 MS.gene035577:intron 40.0%
! AAGAACACGTGTCACTGTTA+AGG - chr8.4:2200719-2200738 None:intergenic 40.0%
! AGAACACGTGTCACTGTTAA+GGG - chr8.4:2200718-2200737 None:intergenic 40.0%
! CCTTTTGGATCAATGTGGAA+GGG + chr8.4:2198511-2198530 MS.gene035577:intron 40.0%
! GACTAATCTCTCTGTTTTCG+TGG - chr8.4:2200553-2200572 None:intergenic 40.0%
! TCATCATTTTCAACCTCCTC+AGG + chr8.4:2198023-2198042 MS.gene035577:CDS 40.0%
! TCCTTTTGGATCAATGTGGA+AGG + chr8.4:2198510-2198529 MS.gene035577:intron 40.0%
! TTTGCCTTGCAAGTATTGCA+TGG - chr8.4:2200893-2200912 None:intergenic 40.0%
!! CCACTTTTAATGTAGTGTGG+CGG + chr8.4:2199649-2199668 MS.gene035577:intron 40.0%
!!! ACGCCACTTTTAATGTAGTG+TGG + chr8.4:2199646-2199665 MS.gene035577:intron 40.0%
!!! ACGTGTTCTTTTTTCCTTGC+AGG + chr8.4:2200729-2200748 MS.gene035577:intron 40.0%
!!! ACTTTTGGGTTGTCGTTTTG+TGG - chr8.4:2200659-2200678 None:intergenic 40.0%
!!! CGTGTTCTTTTTTCCTTGCA+GGG + chr8.4:2200730-2200749 MS.gene035577:intron 40.0%
AACCCTATCACCGCATCTAA+CGG + chr8.4:2201021-2201040 MS.gene035577:CDS 45.0%
AAGCGACACTCGTCTTGAAA+CGG - chr8.4:2198853-2198872 None:intergenic 45.0%
ACCGTTGAAGAGAGCTTGAA+CGG - chr8.4:2197971-2197990 None:intergenic 45.0%
AGGGGAATCTCACTTTGACT+TGG - chr8.4:2199950-2199969 None:intergenic 45.0%
AGTGACTGCGTCCAAACAAA+CGG + chr8.4:2200757-2200776 MS.gene035577:CDS 45.0%
ATCGATGATCTCATCCAGCA+TGG - chr8.4:2199907-2199926 None:intergenic 45.0%
CATTGTGAGGCTTTCGTTAG+AGG - chr8.4:2200824-2200843 None:intergenic 45.0%
CCCTTCCACATTGATCCAAA+AGG - chr8.4:2198514-2198533 None:intergenic 45.0%
CCGCCACACTACATTAAAAG+TGG - chr8.4:2199652-2199671 None:intergenic 45.0%
TCCGTTCAAGCTCTCTTCAA+CGG + chr8.4:2197967-2197986 MS.gene035577:CDS 45.0%
TCTGCCATGCAATACTTGCA+AGG + chr8.4:2200886-2200905 MS.gene035577:CDS 45.0%
TGAGAGGATGAAAGCTTTGC+AGG + chr8.4:2198600-2198619 MS.gene035577:CDS 45.0%
! ATGCTGTTAACGGACATTCC+GGG - chr8.4:2201063-2201082 None:intergenic 45.0%
! GAACACGTGTCACTGTTAAG+GGG - chr8.4:2200717-2200736 None:intergenic 45.0%
! GATGCTGTTAACGGACATTC+CGG - chr8.4:2201064-2201083 None:intergenic 45.0%
!! TAGAGGACGACGCTGTTTTA+GGG - chr8.4:2200807-2200826 None:intergenic 45.0%
!! TCACTTTGACTTGGACCTGT+AGG - chr8.4:2199941-2199960 None:intergenic 45.0%
!! TTAGAGGACGACGCTGTTTT+AGG - chr8.4:2200808-2200827 None:intergenic 45.0%
!!! ACGCTGTTTTAGGGTTTCGT+GGG - chr8.4:2200798-2200817 None:intergenic 45.0%
!!! TAGCTACGCGTGCTACTTTT+GGG - chr8.4:2200673-2200692 None:intergenic 45.0%
!!! TTTTTTTATCATAAAATTTA+TGG + chr8.4:2199728-2199747 MS.gene035577:intron 5.0%
AGGTGTTAACGACGTCGTTC+TGG - chr8.4:2197833-2197852 None:intergenic 50.0%
AGTCAAAGTCAGCAGGCATG+TGG - chr8.4:2197806-2197825 None:intergenic 50.0%
ATCACCGCATCTAACGGTGA+TGG + chr8.4:2201027-2201046 MS.gene035577:CDS 50.0%
CACCGTTAGATGCGGTGATA+GGG - chr8.4:2201026-2201045 None:intergenic 50.0%
CTTGATGCTCCGTCATTGTG+AGG - chr8.4:2200837-2200856 None:intergenic 50.0%
GACACTCGTCTTGAAACGGA+GGG - chr8.4:2198849-2198868 None:intergenic 50.0%
GAGGACATATCAGCCACAAG+AGG - chr8.4:2200224-2200243 None:intergenic 50.0%
GCATCAAGTGGCCAAGCTAA+TGG + chr8.4:2200849-2200868 MS.gene035577:CDS 50.0%
GCTCTCTTCAACGGTTTCTC+CGG + chr8.4:2197976-2197995 MS.gene035577:CDS 50.0%
GGAGGGAAAGAATTGCTGAG+AGG + chr8.4:2198584-2198603 MS.gene035577:CDS 50.0%
GGTGTTAACGACGTCGTTCT+GGG - chr8.4:2197832-2197851 None:intergenic 50.0%
TCACCGCATCTAACGGTGAT+GGG + chr8.4:2201028-2201047 MS.gene035577:CDS 50.0%
TCACCGTTAGATGCGGTGAT+AGG - chr8.4:2201027-2201046 None:intergenic 50.0%
TCACTGTTAAGGGGAGAGAG+TGG - chr8.4:2200708-2200727 None:intergenic 50.0%
TCAGCCAAGGCAGAAGGTTA+GGG + chr8.4:2198211-2198230 MS.gene035577:CDS 50.0%
TCCAAACAAACGGCAACGGA+GGG + chr8.4:2200767-2200786 MS.gene035577:CDS 50.0%
TGCGTCCAAACAAACGGCAA+CGG + chr8.4:2200763-2200782 MS.gene035577:CDS 50.0%
TGTGGATCGACAGACAGACA+AGG + chr8.4:2199878-2199897 MS.gene035577:intron 50.0%
! AGCTTGGTTCGTTGCACTCA+CGG - chr8.4:2198171-2198190 None:intergenic 50.0%
! CACAATGACGGAGCATCAAG+TGG + chr8.4:2200837-2200856 MS.gene035577:CDS 50.0%
! GAACGATAGGGGCAGAGTTT+TGG + chr8.4:2198137-2198156 MS.gene035577:intron 50.0%
!! AGCGTTGATCAAAGGGTTCC+TGG - chr8.4:2200974-2200993 None:intergenic 50.0%
!!! CTGTTTTAGGGTTTCGTGGG+TGG - chr8.4:2200795-2200814 None:intergenic 50.0%
!!! GACGCTGTTTTAGGGTTTCG+TGG - chr8.4:2200799-2200818 None:intergenic 50.0%
!!! GTAGCTACGCGTGCTACTTT+TGG - chr8.4:2200674-2200693 None:intergenic 50.0%
!!! TAGGGGCAGAGTTTTGGAAG+TGG + chr8.4:2198143-2198162 MS.gene035577:intron 50.0%
!!! TGTTAGCTTTTGCAGGCTGC+TGG + chr8.4:2197937-2197956 MS.gene035577:intron 50.0%
AGGGACACCATGGAGAGATC+CGG - chr8.4:2197998-2198017 None:intergenic 55.0%
ATGAGCAGATTAGGGGGAGC+AGG + chr8.4:2200188-2200207 MS.gene035577:CDS 55.0%
CAAAGTCAGCAGGCATGTGG+AGG - chr8.4:2197803-2197822 None:intergenic 55.0%
CAAGGCAGAAGGTTAGGGCA+AGG + chr8.4:2198216-2198235 MS.gene035577:CDS 55.0%
CAGAAGGTTAGGGCAAGGAG+AGG + chr8.4:2198221-2198240 MS.gene035577:CDS 55.0%
CCAAACAAACGGCAACGGAG+GGG + chr8.4:2200768-2200787 MS.gene035577:CDS 55.0%
CGACACTCGTCTTGAAACGG+AGG - chr8.4:2198850-2198869 None:intergenic 55.0%
CTCAGCCAAGGCAGAAGGTT+AGG + chr8.4:2198210-2198229 MS.gene035577:CDS 55.0%
CTTGCCCTAACCTTCTGCCT+TGG - chr8.4:2198218-2198237 None:intergenic 55.0%
GACAGACAAGGCTTCCATGC+TGG + chr8.4:2199890-2199909 MS.gene035577:intron 55.0%
GAGTGCAACGAACCAAGCTC+CGG + chr8.4:2198172-2198191 MS.gene035577:CDS 55.0%
GATGAGGTTGAGGGACACCA+TGG - chr8.4:2198008-2198027 None:intergenic 55.0%
GCGTAGGCCCTCCCAAATAA+GGG - chr8.4:2198446-2198465 None:intergenic 55.0%
GGTTTCTCCGGATCTCTCCA+TGG + chr8.4:2197988-2198007 MS.gene035577:CDS 55.0%
GTCCAAACAAACGGCAACGG+AGG + chr8.4:2200766-2200785 MS.gene035577:CDS 55.0%
TGAGCAGATTAGGGGGAGCA+GGG + chr8.4:2200189-2200208 MS.gene035577:CDS 55.0%
TGGCCCATCACCGTTAGATG+CGG - chr8.4:2201034-2201053 None:intergenic 55.0%
TGGGAGGAGTCAAAGTCAGC+AGG - chr8.4:2197813-2197832 None:intergenic 55.0%
TGTGGCTGATATGTCCTCCG+AGG + chr8.4:2200226-2200245 MS.gene035577:CDS 55.0%
! GTTAACGACGTCGTTCTGGG+AGG - chr8.4:2197829-2197848 None:intergenic 55.0%
! GTTAACGGACATTCCGGGAG+AGG - chr8.4:2201058-2201077 None:intergenic 55.0%
!! ATGCTTCTCACCGCCGATTC+CGG + chr8.4:2197771-2197790 MS.gene035577:CDS 55.0%
ACCCACACAGCATCGCCGAA+AGG + chr8.4:2198255-2198274 MS.gene035577:CDS 60.0%
ACCCTTTCGGCGATGCTGTG+TGG - chr8.4:2198260-2198279 None:intergenic 60.0%
ACCTTCTGCCTTGGCTGAGC+CGG - chr8.4:2198209-2198228 None:intergenic 60.0%
ACGGACATTCCGGGAGAGGA+TGG - chr8.4:2201054-2201073 None:intergenic 60.0%
AGGCATGTGGAGGAGACCTC+CGG - chr8.4:2197793-2197812 None:intergenic 60.0%
AGGGGCTGTAGCTCCTCTTG+TGG + chr8.4:2200208-2200227 MS.gene035577:CDS 60.0%
AGGGTTCCTGGTGTGACACG+TGG - chr8.4:2200962-2200981 None:intergenic 60.0%
CAACTGCCACGTGTCACACC+AGG + chr8.4:2200953-2200972 MS.gene035577:CDS 60.0%
CCCACACAGCATCGCCGAAA+GGG + chr8.4:2198256-2198275 MS.gene035577:CDS 60.0%
CCCTTTCGGCGATGCTGTGT+GGG - chr8.4:2198259-2198278 None:intergenic 60.0%
GAGCAGATTAGGGGGAGCAG+GGG + chr8.4:2200190-2200209 MS.gene035577:CDS 60.0%
GCAGTCACTGACACCCTGCA+AGG - chr8.4:2200746-2200765 None:intergenic 60.0%
GGCGTAGGCCCTCCCAAATA+AGG - chr8.4:2198447-2198466 None:intergenic 60.0%
GTGGAGGAGACCTCCGGAAT+CGG - chr8.4:2197787-2197806 None:intergenic 60.0%
TTCGGCGATGCTGTGTGGGT+CGG - chr8.4:2198255-2198274 None:intergenic 60.0%
! CCCCTCCGTTGCCGTTTGTT+TGG - chr8.4:2200771-2200790 None:intergenic 60.0%
ACGAACCAAGCTCCGGCGAG+TGG + chr8.4:2198179-2198198 MS.gene035577:CDS 65.0%
GAGGAGACCTCCGGAATCGG+CGG - chr8.4:2197784-2197803 None:intergenic 65.0%
TCCGGCTCAGCCAAGGCAGA+AGG + chr8.4:2198205-2198224 MS.gene035577:CDS 65.0%
!! CTTCTCACCGCCGATTCCGG+AGG + chr8.4:2197774-2197793 MS.gene035577:CDS 65.0%
!! GGTGATGGGCCATCCTCTCC+CGG + chr8.4:2201042-2201061 MS.gene035577:CDS 65.0%
AGCTCCGGCGAGTGGAGCTC+CGG + chr8.4:2198187-2198206 MS.gene035577:CDS 70.0%
GAGCTCCACTCGCCGGAGCT+TGG - chr8.4:2198187-2198206 None:intergenic 70.0%
GGCGATGCTGTGTGGGTCGG+TGG - chr8.4:2198252-2198271 None:intergenic 70.0%
GTGGAGCTCCGGCTCAGCCA+AGG + chr8.4:2198198-2198217 MS.gene035577:CDS 70.0%
TGAGCCGGAGCTCCACTCGC+CGG - chr8.4:2198194-2198213 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 2197771 2201116 2197771 ID=MS.gene035577
chr8.4 mRNA 2197771 2201116 2197771 ID=MS.gene035577.t1;Parent=MS.gene035577
chr8.4 exon 2197771 2197857 2197771 ID=MS.gene035577.t1.exon1;Parent=MS.gene035577.t1
chr8.4 CDS 2197771 2197857 2197771 ID=cds.MS.gene035577.t1;Parent=MS.gene035577.t1
chr8.4 exon 2197952 2198044 2197952 ID=MS.gene035577.t1.exon2;Parent=MS.gene035577.t1
chr8.4 CDS 2197952 2198044 2197952 ID=cds.MS.gene035577.t1;Parent=MS.gene035577.t1
chr8.4 exon 2198146 2198277 2198146 ID=MS.gene035577.t1.exon3;Parent=MS.gene035577.t1
chr8.4 CDS 2198146 2198277 2198146 ID=cds.MS.gene035577.t1;Parent=MS.gene035577.t1
chr8.4 exon 2198580 2198645 2198580 ID=MS.gene035577.t1.exon4;Parent=MS.gene035577.t1
chr8.4 CDS 2198580 2198645 2198580 ID=cds.MS.gene035577.t1;Parent=MS.gene035577.t1
chr8.4 exon 2199891 2199956 2199891 ID=MS.gene035577.t1.exon5;Parent=MS.gene035577.t1
chr8.4 CDS 2199891 2199956 2199891 ID=cds.MS.gene035577.t1;Parent=MS.gene035577.t1
chr8.4 exon 2200179 2200247 2200179 ID=MS.gene035577.t1.exon6;Parent=MS.gene035577.t1
chr8.4 CDS 2200179 2200247 2200179 ID=cds.MS.gene035577.t1;Parent=MS.gene035577.t1
chr8.4 exon 2200751 2201116 2200751 ID=MS.gene035577.t1.exon7;Parent=MS.gene035577.t1
chr8.4 CDS 2200751 2201116 2200751 ID=cds.MS.gene035577.t1;Parent=MS.gene035577.t1
Gene Sequence

>MS.gene035577

ATGCTTCTCACCGCCGATTCCGGAGGTCTCCTCCACATGCCTGCTGACTTTGACTCCTCCCAGAACGACGTCGTTAACACCTCCTCCGTGAGTATATTCATATTTTCTCACTCCGTTAATTAATTAACAACGTTATCGTTTTTATTACTACTGAATATTTTAATATTGTTAGCTTTTGCAGGCTGCTGGTGACGCTTCCGTTCAAGCTCTCTTCAACGGTTTCTCCGGATCTCTCCATGGTGTCCCTCAACCTCATCATTTTCAACCTCCTCAGGTTCTATTCTTTCTTAGTTTTTCAAACAAAATCATTGAAATATTTTTCATGTGCATGTAAATTGATTTGATTTATTTCTTTTTTGGGAATTTGAACGATAGGGGCAGAGTTTTGGAAGTGGTTCCGTGAGTGCAACGAACCAAGCTCCGGCGAGTGGAGCTCCGGCTCAGCCAAGGCAGAAGGTTAGGGCAAGGAGAGGTCAAGCCACCGACCCACACAGCATCGCCGAAAGGGTATGTATCTCACTATAACGTGTTTGGTTGTGCAGAAAATTGGGTACAATAAATAACTTAAGTTGTCATTTTCAGTGTAGCAATTAATATACTTATGAGAACGCCAAAACGTGCAATTTTTTCAGAGAAAATGCGGTTGTTTGTCAGTGGCTAACAGAATATAAACCCTTATTTGGGAGGGCCTACGCCTACTATCATTCTGTTTCTGTTTTTAAAATCTCTTTCTTTTGTTTCCTTTTGGATCAATGTGGAAGGGTAATAGTACAATGGTAATTTGGTAATTTTGTTTTTATGTATGGCAGTTACGGAGGGAAAGAATTGCTGAGAGGATGAAAGCTTTGCAGGAACTTGTTCCTAATGCAAACAAGGTAACACTAGACTTAATTTAATACTCAATTATGGCTTATTTTGTTGATTATCATGAGTAAGTAAGAGTGATATTCAATTTCAATCATCATGTTATATTGTACTTAACAAAAAAAAAAAAAATGTTTCATGCATGTCCAATTTTCTCATGTGATTAATTTAGTTGAGTATATGATTATCCCTCAAAAAAAAGTTTACTACTCCCTCCGTTTCAAGACGAGTGTCGCTTTAGCCAATTGCACACAGATTAAGGAATGAAATAAAGTAGTCAAATGTCATAGCAATTTTATCAAATTATCCTAATCAACTATTAGTTTGTTTTTCATTAAAGAAAAATAATACTTTGGAAGTAGTGTAACATAGGATTAATTGAATGGTACAATTGAAAAGAAAAAGTTATTATTGCATTGGAAACGTAAAGTGACATTCATTTTAAAACAATTTTTTTTTGCTAAAGCGACACTCATTTTGAAACAGATAGAGTATATGATTAATCATCATGTTTTTTTTTGACACAAATCTGGATCATGTTAAAATTTGTGAAATTGTAGTAAATCATGTAGTAATTGAATTAACCACAATTTTAATCTAAATTACATTTGATATCTCCTCGATTGAATTAACCACAACTCTAAACATCATGTACCAAAAAACAGTGTATTAAAATCATAATAACTCTTTAGTTAGATAATGAATACTCTATTTCATCCCCGACAATTTTTTGAGCATAATTTTGTGTCTGACAAAAAATATCAAAATAGCCTGTGATATTTTCAATTTGTAGACAAATTAACTATTCTGTTACAACTTCTAGCCTTATTAAAAAAAGAAAACTATTCTGTTACAACGAGTCGTAAACTAATTTTTTCATTGTATGAAAATATCAGAGGACTAATCTGATATCTTTTTTTGTAAGGAAGTCAATTGGGTCGGAAAAAATTGTGATCGATCAAATTAAGTATTTTTTCTTAATTGACTGAATGCGGTTCATTACTTGTAAGCACGCCACTTTTAATGTAGTGTGGCGGTGATATACTTTCATTCGTGCGTTTACATGTCCACTTGTTTTAGCTGATTGAGCTTATTTTTTTATCATAAAATTTATGGCCTCAATAATTTTCTCAAGATGTGGTTATTAATTAATCAATTTAAAAACATTTAAATATAAGACACTTATAAACATAATGTAATGGGTGTATCTATCCATGTTGAAATTTTAATTATTTGTTTGGTCTGTGGATCGACAGACAGACAAGGCTTCCATGCTGGATGAGATCATCGATTATGTGAAGTTCCTACAGGTCCAAGTCAAAGTGAGATTCCCCTCTTTTTCTTCAATATACTTTTGGGAAATTTACAAAATACATTTTTCACTTAAAATGTCTTGTAATATCTATATCTATATAATTTCAACAATTTTTTTTCTTCTGTTGTTTCATGACTTAATAATGTTATTACGTTTCTCAAGTTAACTTATTTCAAGGATTCTTTGTTTGAAATAATTTTTGTTACCTGTTGCGTAATTGGATTTACAGGTTTTGAGTATGAGCAGATTAGGGGGAGCAGGGGCTGTAGCTCCTCTTGTGGCTGATATGTCCTCCGAGGTAAATTTTCCACTTTCTTTCTGAATTTACCAAAGTTATTATTATTTATAGTAAAAAAAGAAACTTTTCTCGTGGCTTTCTTGTTGCCATTGATCTTTCAAAAAAGAAAATAAACAATCCCACTTTTCTTCTGCTTTTTCTGATTGCTTGTTTCTTCCTAATTCAATGGAATAAAAAATAATCAAGCTCCTATTACAATTTATTTTTATCTTTCCCTACCATAAATTGAGAAAGCAAGAAATTGAGGAAAGTATTAAAAAAACATGTTAGAATTTCCATAAGAAGAAATAAATACTCAGCATCCACGAAAACAGAGAGATTAGTCTTCTCACAAGATGACACACCAACTTTATTTTTTGTTAGCTTTGTTTCGCAGTATTAATCAATCAATTCTGAAATTCTCAATCACCACAAAACGACAACCCAAAAGTAGCACGCGTAGCTACTTTCTCAGATTCCACTCTCTCCCCTTAACAGTGACACGTGTTCTTTTTTCCTTGCAGGGTGTCAGTGACTGCGTCCAAACAAACGGCAACGGAGGGGTCCACCCACGAAACCCTAAAACAGCGTCGTCCTCTAACGAAAGCCTCACAATGACGGAGCATCAAGTGGCCAAGCTAATGGAAGAAGACATGGGATCTGCCATGCAATACTTGCAAGGCAAAGGTCTTTGCCTCATGCCTATTTCTCTTGCTACTGCTATCTCAACTGCCACGTGTCACACCAGGAACCCTTTGATCAACGCTGCTAATAACAATATCAACGGTAGTTCTAACCCTATCACCGCATCTAACGGTGATGGGCCATCCTCTCCCGGAATGTCCGTTAACAGCATCGTCAAAGATGCTAACTCCGCTTCTAAGTCGTAG
Protein sequence

>MS.gene035577.t1

MLLTADSGGLLHMPADFDSSQNDVVNTSSAAGDASVQALFNGFSGSLHGVPQPHHFQPPQGQSFGSGSVSATNQAPASGAPAQPRQKVRARRGQATDPHSIAERLRRERIAERMKALQELVPNANKTDKASMLDEIIDYVKFLQVQVKVLSMSRLGGAGAVAPLVADMSSEGVSDCVQTNGNGGVHPRNPKTASSSNESLTMTEHQVAKLMEEDMGSAMQYLQGKGLCLMPISLATAISTATCHTRNPLINAANNNINGSSNPITASNGDGPSSPGMSVNSIVKDANSASKS