Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03716.t1 | XP_003606312.1 | 100 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 2.50E-44 | 187.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03716.t1 | O23249 | 94.3 | 88 | 4 | 1 | 1 | 88 | 1 | 87 | 2.4e-43 | 175.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03716.t1 | G7JM76 | 100.0 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 1.8e-44 | 187.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene03716 | MS.gene031852 | PPI |
| MS.gene03716 | MS.gene036003 | PPI |
| MS.gene73923 | MS.gene03716 | PPI |
| MS.gene03716 | MS.gene54070 | PPI |
| MS.gene03716 | MS.gene021573 | PPI |
| MS.gene03716 | MS.gene053749 | PPI |
| MS.gene03716 | MS.gene30776 | PPI |
| MS.gene03716 | MS.gene29062 | PPI |
| MS.gene03716 | MS.gene94095 | PPI |
| MS.gene03716 | MS.gene86296 | PPI |
| MS.gene03716 | MS.gene015893 | PPI |
| MS.gene03716 | MS.gene57583 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03716.t1 | MTR_4g057040 | 100.000 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 1.71e-62 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03716.t1 | AT2G27970 | 90.000 | 80 | 8 | 0 | 1 | 80 | 1 | 80 | 1.45e-51 | 156 |
| MS.gene03716.t1 | AT2G27960 | 97.183 | 71 | 2 | 0 | 1 | 71 | 1 | 71 | 5.79e-49 | 150 |
Find 27 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGGACAACCAGGCCCAGC+AGG | 0.260365 | 4.3:+44061179 | MS.gene03716:CDS |
| CCAGCATGCCCTGCTGGGCC+TGG | 0.333404 | 4.3:-44061188 | None:intergenic |
| AGCCACACATTATGCTCTTC+AGG | 0.335634 | 4.3:+44061133 | MS.gene03716:CDS |
| TTCAGAGAGAAGTCGATTCT+TGG | 0.337969 | 4.3:-44058947 | None:intergenic |
| ATTTGACCAGCATGCCCTGC+TGG | 0.343644 | 4.3:-44061194 | None:intergenic |
| GAGTGCAGCAGAGCCGTGGC+TGG | 0.380036 | 4.3:+44061085 | MS.gene03716:CDS |
| CAGAATGAATGGCGCGCAAT+TGG | 0.392088 | 4.3:+44061063 | MS.gene03716:intron |
| GGAAGCAACTTCGCAACCTC+TGG | 0.415067 | 4.3:-44058925 | None:intergenic |
| CGAAATACTTCTCGGAGTAC+TGG | 0.418536 | 4.3:-44058761 | None:intergenic |
| GTTTAATCTTACAGAATGAA+TGG | 0.420697 | 4.3:+44061052 | MS.gene03716:intron |
| TTTGACCAGCATGCCCTGCT+GGG | 0.452510 | 4.3:-44061193 | None:intergenic |
| TCAGAGAGAAGTCGATTCTT+GGG | 0.456322 | 4.3:-44058946 | None:intergenic |
| CCAGGCCCAGCAGGGCATGC+TGG | 0.487057 | 4.3:+44061188 | MS.gene03716:CDS |
| TGCTGCTGCTGATAATTCAA+TGG | 0.507626 | 4.3:-44061159 | None:intergenic |
| AGTGTCATCGAAATACTTCT+CGG | 0.516378 | 4.3:-44058769 | None:intergenic |
| CAGGACAACCAGGCCCAGCA+GGG | 0.518346 | 4.3:+44061180 | MS.gene03716:CDS |
| AGTGCAGCAGAGCCGTGGCT+GGG | 0.520271 | 4.3:+44061086 | MS.gene03716:CDS |
| AAGAGCATAATGTGTGGCTC+AGG | 0.531062 | 4.3:-44061129 | None:intergenic |
| TCGATGACACTTATGAGTAC+AGG | 0.536322 | 4.3:+44058781 | MS.gene03716:CDS |
| GCATGTTGTTCTTCCTCCAG+AGG | 0.545874 | 4.3:+44058909 | MS.gene03716:CDS |
| AGCAACTTCGCAACCTCTGG+AGG | 0.549766 | 4.3:-44058922 | None:intergenic |
| ATTGAATTATCAGCAGCAGC+AGG | 0.557262 | 4.3:+44061161 | MS.gene03716:CDS |
| ATTGGAGTGCAGCAGAGCCG+TGG | 0.573620 | 4.3:+44061081 | MS.gene03716:CDS |
| TCAGCAGCAGCAGGACAACC+AGG | 0.580092 | 4.3:+44061170 | MS.gene03716:CDS |
| TTGCGTAATGAACCCAGCCA+CGG | 0.630137 | 4.3:-44061098 | None:intergenic |
| CACACATTATGCTCTTCAGG+AGG | 0.695794 | 4.3:+44061136 | MS.gene03716:CDS |
| CTCCTGAAGAGCATAATGTG+TGG | 0.729925 | 4.3:-44061135 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCAAAAAAAAAAAAAAACT+AGG | - | chr4.3:44058847-44058866 | None:intergenic | 10.0% |
| !!! | ATTGTTTTATTATTTTTTTG+TGG | + | chr4.3:44059153-44059172 | MS.gene03716:intron | 10.0% |
| !!! | TATTTGTAAAATTTAAGTTA+AGG | - | chr4.3:44060562-44060581 | None:intergenic | 10.0% |
| !! | AGTTATCAATTAAAAGTTTA+AGG | - | chr4.3:44059819-44059838 | None:intergenic | 15.0% |
| !! | ATTAAATACACAATGAATTT+AGG | - | chr4.3:44060507-44060526 | None:intergenic | 15.0% |
| !! | CAAAAAAAATATTATAAGTC+TGG | - | chr4.3:44059388-44059407 | None:intergenic | 15.0% |
| !!! | AATTAAAAGTTTAAGGTAAT+CGG | - | chr4.3:44059812-44059831 | None:intergenic | 15.0% |
| !!! | AATTATCGATTAAAAGTTTA+AGG | - | chr4.3:44060115-44060134 | None:intergenic | 15.0% |
| !!! | ATTGTTTTTGTAAATAAGTA+CGG | + | chr4.3:44060896-44060915 | MS.gene03716:intron | 15.0% |
| !!! | ATTTGTAAAATTTAAGTTCA+AGG | - | chr4.3:44060720-44060739 | None:intergenic | 15.0% |
| !!! | TCTTTGAAGATATTATATTT+TGG | + | chr4.3:44059124-44059143 | MS.gene03716:intron | 15.0% |
| !! | AGCTATCAATTAAAAGTTTA+AGG | - | chr4.3:44059847-44059866 | None:intergenic | 20.0% |
| !! | GTTTGTTTAAATATTCTGTT+TGG | + | chr4.3:44061030-44061049 | MS.gene03716:intron | 20.0% |
| !! | TAGAGATGTATATTTGAATT+TGG | + | chr4.3:44059640-44059659 | MS.gene03716:intron | 20.0% |
| !!! | AATTATCAATCAAAAGTGTA+AGG | - | chr4.3:44060313-44060332 | None:intergenic | 20.0% |
| !!! | ATCAGTTAAAAGTTTAAGTT+AGG | - | chr4.3:44059787-44059806 | None:intergenic | 20.0% |
| !!! | TATGTCTTAGAGTTTATTTT+AGG | + | chr4.3:44060204-44060223 | MS.gene03716:intron | 20.0% |
| !!! | TGAAGACTTAAATTTGATTT+TGG | + | chr4.3:44059239-44059258 | MS.gene03716:intron | 20.0% |
| !!! | TTAATTGATAGCTTAATATG+TGG | + | chr4.3:44059854-44059873 | MS.gene03716:intron | 20.0% |
| !!! | TTTGTTTTATGCTGAATTTT+TGG | + | chr4.3:44059072-44059091 | MS.gene03716:intron | 20.0% |
| !!! | TTTTTTTTGTTGCTGTTTTA+CGG | + | chr4.3:44059399-44059418 | MS.gene03716:intron | 20.0% |
| ! | AACCAAATTAAGAATCACTT+TGG | - | chr4.3:44060180-44060199 | None:intergenic | 25.0% |
| ! | AGGAAAAGTTCATATTGAAA+GGG | - | chr4.3:44059767-44059786 | None:intergenic | 25.0% |
| ! | GTCATAAAAATCATGTTTAG+AGG | - | chr4.3:44060486-44060505 | None:intergenic | 25.0% |
| ! | GTTTAATCTTACAGAATGAA+TGG | + | chr4.3:44061052-44061071 | MS.gene03716:intron | 25.0% |
| ! | TAGGAAAAGTTCATATTGAA+AGG | - | chr4.3:44059768-44059787 | None:intergenic | 25.0% |
| ! | TCAAAATAGATAATACCCTA+AGG | - | chr4.3:44059520-44059539 | None:intergenic | 25.0% |
| ! | TCAGATTGTTAAACAATGAA+GGG | + | chr4.3:44060812-44060831 | MS.gene03716:intron | 25.0% |
| ! | TCGAGACTTATATTTGAATT+TGG | + | chr4.3:44059926-44059945 | MS.gene03716:intron | 25.0% |
| ! | TCTCAGTGTATTTAGATATT+GGG | - | chr4.3:44060248-44060267 | None:intergenic | 25.0% |
| ! | TTCAGATTGTTAAACAATGA+AGG | + | chr4.3:44060811-44060830 | MS.gene03716:intron | 25.0% |
| ! | TTCTCAGTGTATTTAGATAT+TGG | - | chr4.3:44060249-44060268 | None:intergenic | 25.0% |
| !! | AAGGTAACATGATTTAAGTT+TGG | - | chr4.3:44060294-44060313 | None:intergenic | 25.0% |
| !! | ATTGATTCTAACAGAAAGTT+TGG | + | chr4.3:44059704-44059723 | MS.gene03716:intron | 25.0% |
| !! | CAAACAGTCTTTTCATTAAA+TGG | - | chr4.3:44061015-44061034 | None:intergenic | 25.0% |
| !! | TTATGATGATGACTTAATCT+TGG | + | chr4.3:44059488-44059507 | MS.gene03716:intron | 25.0% |
| !!! | AATGTTAATTTGTGCATGTT+AGG | + | chr4.3:44059189-44059208 | MS.gene03716:intron | 25.0% |
| !!! | AGTGATTCTTAATTTGGTTT+TGG | + | chr4.3:44060181-44060200 | MS.gene03716:intron | 25.0% |
| !!! | ATGATTTTTGGGTTTTGTTA+TGG | + | chr4.3:44060964-44060983 | MS.gene03716:intron | 25.0% |
| !!! | ATGTTAATTTGTGCATGTTA+GGG | + | chr4.3:44059190-44059209 | MS.gene03716:intron | 25.0% |
| !!! | TGATTTTTGGGTTTTGTTAT+GGG | + | chr4.3:44060965-44060984 | MS.gene03716:intron | 25.0% |
| !!! | TGTTTCGTTTTTGTTTTTTC+AGG | + | chr4.3:44058887-44058906 | MS.gene03716:intron | 25.0% |
| AAACAACAACTCTTGACTTT+AGG | - | chr4.3:44060460-44060479 | None:intergenic | 30.0% | |
| AAACCAAACACACTTAATCA+AGG | + | chr4.3:44060767-44060786 | MS.gene03716:intron | 30.0% | |
| AAAGTGAATTCAGTGTCTTT+GGG | - | chr4.3:44060019-44060038 | None:intergenic | 30.0% | |
| AACACACTTAATCAAGGAAA+CGG | + | chr4.3:44060773-44060792 | MS.gene03716:intron | 30.0% | |
| AGGTTACATGTATTACGTTA+AGG | - | chr4.3:44060700-44060719 | None:intergenic | 30.0% | |
| AGTTCATTACACAGTGAATT+TGG | - | chr4.3:44059347-44059366 | None:intergenic | 30.0% | |
| GACCAAAGTGATTCTTAATT+TGG | + | chr4.3:44060175-44060194 | MS.gene03716:intron | 30.0% | |
| GATAACTGAATATTACCATG+TGG | + | chr4.3:44059568-44059587 | MS.gene03716:intron | 30.0% | |
| GGTATAAAAATCACGTTTAG+AGG | - | chr4.3:44059325-44059344 | None:intergenic | 30.0% | |
| GTTCATTACACAGTGAATTT+GGG | - | chr4.3:44059346-44059365 | None:intergenic | 30.0% | |
| TAATCTTGGTTAAGTCCTTA+GGG | + | chr4.3:44059502-44059521 | MS.gene03716:intron | 30.0% | |
| TGGTATTATCACTGTTGAAA+GGG | + | chr4.3:44059890-44059909 | MS.gene03716:intron | 30.0% | |
| TTAATCTTGGTTAAGTCCTT+AGG | + | chr4.3:44059501-44059520 | MS.gene03716:intron | 30.0% | |
| TTTCCTTGATTAAGTGTGTT+TGG | - | chr4.3:44060773-44060792 | None:intergenic | 30.0% | |
| ! | AGCTGAAGCAATATGATTTT+TGG | + | chr4.3:44060952-44060971 | MS.gene03716:intron | 30.0% |
| ! | GCTGAAGCAATATGATTTTT+GGG | + | chr4.3:44060953-44060972 | MS.gene03716:intron | 30.0% |
| ! | GTTACTCTGATGATGAAATT+GGG | + | chr4.3:44059094-44059113 | MS.gene03716:intron | 30.0% |
| !! | GTTTGCATTCACTTTTGAAA+GGG | + | chr4.3:44060353-44060372 | MS.gene03716:intron | 30.0% |
| !! | TGTTTGCATTCACTTTTGAA+AGG | + | chr4.3:44060352-44060371 | MS.gene03716:intron | 30.0% |
| !! | TTTGCATTCACTTTTGAAAG+GGG | + | chr4.3:44060354-44060373 | MS.gene03716:intron | 30.0% |
| !!! | AAGTGTGTTTGGTTTTAATG+TGG | - | chr4.3:44060762-44060781 | None:intergenic | 30.0% |
| !!! | GTTGTTTTTGCTTCTAAACA+TGG | + | chr4.3:44060606-44060625 | MS.gene03716:intron | 30.0% |
| !!! | TGTTTGGTTTTAATGTGGAA+TGG | - | chr4.3:44060757-44060776 | None:intergenic | 30.0% |
| AAAGGGAATTCAGTGTCTTT+GGG | - | chr4.3:44059750-44059769 | None:intergenic | 35.0% | |
| AACATGCCAAAATGCAAATG+AGG | - | chr4.3:44060391-44060410 | None:intergenic | 35.0% | |
| AATATTACCCTGTGCATGAT+TGG | + | chr4.3:44060136-44060155 | MS.gene03716:intron | 35.0% | |
| AGTGTCATCGAAATACTTCT+CGG | - | chr4.3:44058772-44058791 | None:intergenic | 35.0% | |
| ATGAGTACAGGTAAACGAAT+CGG | + | chr4.3:44058793-44058812 | MS.gene03716:intron | 35.0% | |
| GAAAGTGAATTCAGTGTCTT+TGG | - | chr4.3:44060020-44060039 | None:intergenic | 35.0% | |
| GGTATTATCACTGTTGAAAG+GGG | + | chr4.3:44059891-44059910 | MS.gene03716:intron | 35.0% | |
| GTGGTATTATCACTGTTGAA+AGG | + | chr4.3:44059889-44059908 | MS.gene03716:intron | 35.0% | |
| TGCTTCAGCTTTGCATTAAA+TGG | - | chr4.3:44060942-44060961 | None:intergenic | 35.0% | |
| ! | GGTTACTCTGATGATGAAAT+TGG | + | chr4.3:44059093-44059112 | MS.gene03716:intron | 35.0% |
| !! | TATGTGGCTTGTTATTGTTG+TGG | + | chr4.3:44059870-44059889 | MS.gene03716:intron | 35.0% |
| !! | TTTGCATTTTGGCATGTTTG+AGG | + | chr4.3:44060393-44060412 | MS.gene03716:intron | 35.0% |
| AAGTGAATCCAATCATGCAC+AGG | - | chr4.3:44060147-44060166 | None:intergenic | 40.0% | |
| AGTGAATCCAATCATGCACA+GGG | - | chr4.3:44060146-44060165 | None:intergenic | 40.0% | |
| ATTGAATTATCAGCAGCAGC+AGG | + | chr4.3:44061161-44061180 | MS.gene03716:CDS | 40.0% | |
| CACAACAATAACATGCCACA+TGG | - | chr4.3:44059586-44059605 | None:intergenic | 40.0% | |
| CATGTGGCATGTTATTGTTG+TGG | + | chr4.3:44059584-44059603 | MS.gene03716:intron | 40.0% | |
| GAAAGGGAATTCAGTGTCTT+TGG | - | chr4.3:44059751-44059770 | None:intergenic | 40.0% | |
| TCGATGACACTTATGAGTAC+AGG | + | chr4.3:44058781-44058800 | MS.gene03716:CDS | 40.0% | |
| TGCTGCTGCTGATAATTCAA+TGG | - | chr4.3:44061162-44061181 | None:intergenic | 40.0% | |
| TTGGCATGTTTGAGGATTCT+AGG | + | chr4.3:44060401-44060420 | MS.gene03716:intron | 40.0% | |
| ! | GCGATTCCTCATTTGCATTT+TGG | + | chr4.3:44060382-44060401 | MS.gene03716:intron | 40.0% |
| ! | TCAGAGAGAAGTCGATTCTT+GGG | - | chr4.3:44058949-44058968 | None:intergenic | 40.0% |
| ! | TTCAGAGAGAAGTCGATTCT+TGG | - | chr4.3:44058950-44058969 | None:intergenic | 40.0% |
| AGCCACACATTATGCTCTTC+AGG | + | chr4.3:44061133-44061152 | MS.gene03716:CDS | 45.0% | |
| CACACATTATGCTCTTCAGG+AGG | + | chr4.3:44061136-44061155 | MS.gene03716:CDS | 45.0% | |
| CGAAATACTTCTCGGAGTAC+TGG | - | chr4.3:44058764-44058783 | None:intergenic | 45.0% | |
| GGTATTCTCGCTGTTGAAAG+AGG | + | chr4.3:44059605-44059624 | MS.gene03716:intron | 45.0% | |
| ! | AAGAGCATAATGTGTGGCTC+AGG | - | chr4.3:44061132-44061151 | None:intergenic | 45.0% |
| ! | CTCCTGAAGAGCATAATGTG+TGG | - | chr4.3:44061138-44061157 | None:intergenic | 45.0% |
| CAGAATGAATGGCGCGCAAT+TGG | + | chr4.3:44061063-44061082 | MS.gene03716:intron | 50.0% | |
| GCATGTTGTTCTTCCTCCAG+AGG | + | chr4.3:44058909-44058928 | MS.gene03716:CDS | 50.0% | |
| TTGCGTAATGAACCCAGCCA+CGG | - | chr4.3:44061101-44061120 | None:intergenic | 50.0% | |
| AGCAACTTCGCAACCTCTGG+AGG | - | chr4.3:44058925-44058944 | None:intergenic | 55.0% | |
| ATTTGACCAGCATGCCCTGC+TGG | - | chr4.3:44061197-44061216 | None:intergenic | 55.0% | |
| GGAAGCAACTTCGCAACCTC+TGG | - | chr4.3:44058928-44058947 | None:intergenic | 55.0% | |
| TTTGACCAGCATGCCCTGCT+GGG | - | chr4.3:44061196-44061215 | None:intergenic | 55.0% | |
| ATTGGAGTGCAGCAGAGCCG+TGG | + | chr4.3:44061081-44061100 | MS.gene03716:CDS | 60.0% | |
| TCAGCAGCAGCAGGACAACC+AGG | + | chr4.3:44061170-44061189 | MS.gene03716:CDS | 60.0% | |
| AGTGCAGCAGAGCCGTGGCT+GGG | + | chr4.3:44061086-44061105 | MS.gene03716:CDS | 65.0% | |
| CAGGACAACCAGGCCCAGCA+GGG | + | chr4.3:44061180-44061199 | MS.gene03716:CDS | 65.0% | |
| GAGTGCAGCAGAGCCGTGGC+TGG | + | chr4.3:44061085-44061104 | MS.gene03716:CDS | 70.0% | |
| GCAGGACAACCAGGCCCAGC+AGG | + | chr4.3:44061179-44061198 | MS.gene03716:CDS | 70.0% | |
| CCAGCATGCCCTGCTGGGCC+TGG | - | chr4.3:44061191-44061210 | None:intergenic | 75.0% | |
| CCAGGCCCAGCAGGGCATGC+TGG | + | chr4.3:44061188-44061207 | MS.gene03716:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 44058750 | 44061218 | 44058750 | ID=MS.gene03716 |
| chr4.3 | mRNA | 44058750 | 44061218 | 44058750 | ID=MS.gene03716.t1;Parent=MS.gene03716 |
| chr4.3 | exon | 44058750 | 44058802 | 44058750 | ID=MS.gene03716.t1.exon1;Parent=MS.gene03716.t1 |
| chr4.3 | CDS | 44058750 | 44058802 | 44058750 | ID=cds.MS.gene03716.t1;Parent=MS.gene03716.t1 |
| chr4.3 | exon | 44058909 | 44058969 | 44058909 | ID=MS.gene03716.t1.exon2;Parent=MS.gene03716.t1 |
| chr4.3 | CDS | 44058909 | 44058969 | 44058909 | ID=cds.MS.gene03716.t1;Parent=MS.gene03716.t1 |
| chr4.3 | exon | 44061066 | 44061218 | 44061066 | ID=MS.gene03716.t1.exon3;Parent=MS.gene03716.t1 |
| chr4.3 | CDS | 44061066 | 44061218 | 44061066 | ID=cds.MS.gene03716.t1;Parent=MS.gene03716.t1 |
| Gene Sequence |
| Protein sequence |
