AlfalfaGEDB | MS.gene03835

M. sativa cultivar XinJiangDaYe / MS.gene03835

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF01428.19 PF01754.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03835.t1	XP_003606132.1	85.3	170	21	1	1	166	1	170	5.70E-74	287

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03835.t1	Q9SJM6	49.4	168	76	2	1	166	1	161	2.0e-38	160.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03835.t1	G7JIG0	85.3	170	21	1	1	166	1	170	4.1e-74	287.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene03835	MS.gene24724	PPI
MS.gene050335	MS.gene03835	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03835.t1	MTR_4g053440	93.529	170	7	2	1	166	1	170	7.06e-114	320
MS.gene03835.t1	MTR_2g086190	56.627	166	56	3	1	166	1	150	3.72e-59	181
MS.gene03835.t1	MTR_7g104320	51.705	176	67	4	1	166	1	168	1.26e-57	178
MS.gene03835.t1	MTR_1g060380	50.595	168	69	3	1	166	1	156	7.90e-47	150
MS.gene03835.t1	MTR_2g098160	41.279	172	82	5	7	166	8	172	7.14e-41	135
MS.gene03835.t1	MTR_0249s0070	42.604	169	88	5	3	166	5	169	1.07e-39	132
MS.gene03835.t1	MTR_0249s0070	42.604	169	88	5	3	166	13	177	1.42e-39	132
MS.gene03835.t1	MTR_7g092400	39.011	182	79	5	3	166	93	260	8.72e-38	130
MS.gene03835.t1	MTR_7g114920	40.789	152	63	5	13	163	5	130	2.28e-31	110
MS.gene03835.t1	MTR_4g065570	37.931	145	75	3	28	166	2	137	3.71e-31	109
MS.gene03835.t1	MTR_1g100773	39.073	151	74	4	13	163	5	137	5.37e-30	107
MS.gene03835.t1	MTR_3g028010	33.117	154	85	2	13	166	28	163	1.77e-24	93.6
MS.gene03835.t1	MTR_8g036980	56.452	62	27	0	105	166	90	151	1.58e-22	88.2
MS.gene03835.t1	MTR_2g054650	37.500	152	61	6	13	163	6	124	1.56e-19	79.7
MS.gene03835.t1	MTR_3g025570	54.545	44	20	0	101	144	4	47	6.62e-12	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03835.t1	AT2G27580	56.627	166	69	2	1	166	1	163	7.10e-56	173
MS.gene03835.t1	AT2G27580	56.627	166	69	2	1	166	1	163	7.10e-56	173
MS.gene03835.t1	AT2G36320	49.405	168	76	2	1	166	1	161	4.75e-51	161
MS.gene03835.t1	AT1G12440	45.161	155	82	1	12	166	17	168	2.35e-44	144
MS.gene03835.t1	AT1G12440	45.161	155	82	1	12	166	17	168	2.35e-44	144
MS.gene03835.t1	AT1G12440	45.161	155	82	1	12	166	17	168	2.35e-44	144
MS.gene03835.t1	AT3G52800	48.276	174	78	3	1	166	1	170	2.60e-43	141
MS.gene03835.t1	AT3G52800	48.276	174	78	3	1	166	1	170	2.60e-43	141
MS.gene03835.t1	AT4G12040	46.012	163	76	5	12	166	17	175	4.88e-42	138
MS.gene03835.t1	AT4G12040	46.012	163	76	5	12	166	17	175	4.88e-42	138
MS.gene03835.t1	AT4G22820	43.478	161	81	4	12	166	20	176	1.27e-40	135
MS.gene03835.t1	AT4G22820	43.478	161	81	4	12	166	20	176	1.27e-40	135
MS.gene03835.t1	AT1G51200	44.099	161	81	4	12	166	16	173	9.60e-38	127
MS.gene03835.t1	AT1G51200	44.099	161	81	4	12	166	16	173	9.60e-38	127
MS.gene03835.t1	AT1G51200	44.099	161	81	4	12	166	16	173	9.60e-38	127
MS.gene03835.t1	AT1G51200	44.099	161	81	4	12	166	16	173	9.60e-38	127
MS.gene03835.t1	AT3G12630	34.211	152	74	4	19	166	31	160	1.88e-24	93.2
MS.gene03835.t1	AT4G25380	33.987	153	69	5	14	166	10	130	2.80e-22	87.0
MS.gene03835.t1	AT4G14225	31.613	155	69	2	13	166	7	125	3.08e-20	81.6

Find 52 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACGTTCTTCATCTGCAAAAT+TGG	0.253435	4.1:+44727935	MS.gene03835:CDS
GCTAACAACTGCGGTTTCTT+TGG	0.331458	4.1:+44727852	MS.gene03835:CDS
TATCCGGAGCAGCACGGTTG+TGG	0.350405	4.1:+44728206	MS.gene03835:CDS
CTGCAAATCACGGTAACATT+TGG	0.369389	4.1:-44727901	None:intergenic
CACCTATTCGGCTGGACCTT+CGG	0.371593	4.1:-44728107	None:intergenic
AGCTTCTCTCCCTTCACCAC+TGG	0.384998	4.1:-44728275	None:intergenic
GTGGAGTTGGTAGCTGCTGC+TGG	0.406487	4.1:+44728065	MS.gene03835:CDS
CTACATGTCCCACACCTATT+CGG	0.409919	4.1:-44728119	None:intergenic
CACCGAAGGTCCAGCCGAAT+AGG	0.435160	4.1:+44728105	MS.gene03835:CDS
GTGTGGGACCCACAGGTATC+CGG	0.436288	4.1:+44728190	MS.gene03835:CDS
GAGCAAAGATCTTGCATGGC+AGG	0.449620	4.1:-44727879	None:intergenic
AACCCACAACCGTGCTGCTC+CGG	0.450007	4.1:-44728209	None:intergenic
AGACGGCGCGTGGGGCTGAC+AGG	0.474654	4.1:+44728140	MS.gene03835:CDS
ACTGTTGAGAAATCAACGTT+TGG	0.474698	4.1:-44727965	None:intergenic
TGGGACATGTAGACGGCGCG+TGG	0.476667	4.1:+44728130	MS.gene03835:CDS
ATCCGGAGCAGCACGGTTGT+GGG	0.489485	4.1:+44728207	MS.gene03835:CDS
ATGTCCCACACCTATTCGGC+TGG	0.501533	4.1:-44728115	None:intergenic
TTACCGTGATTTGCAGATGA+AGG	0.514621	4.1:+44727908	MS.gene03835:CDS
AGCTGCTGCTGGACCTTCAG+AGG	0.522810	4.1:+44728076	MS.gene03835:CDS
TTCGGTGGTTCAGCCTCTGA+AGG	0.527668	4.1:-44728089	None:intergenic
ACTACAACCGACGGTGGAGA+TGG	0.535288	4.1:-44728023	None:intergenic
CGTGCTGCTCCGGATACCTG+TGG	0.543879	4.1:-44728199	None:intergenic
GAAGGCGAATCCAGTGGTGA+AGG	0.546816	4.1:+44728265	MS.gene03835:CDS
ATCTACACCGACGGTGGAGT+TGG	0.547793	4.1:+44728052	MS.gene03835:CDS
TGACGTTGTGTGGGACCCAC+AGG	0.547943	4.1:+44728183	MS.gene03835:CDS
CAGGATTTAAGTGTCGGTGC+GGG	0.548307	4.1:+44728159	MS.gene03835:CDS
CGGTGCGGGTTGACGTTGTG+TGG	0.556113	4.1:+44728173	MS.gene03835:CDS
GGCTGACAGGATTTAAGTGT+CGG	0.567077	4.1:+44728153	MS.gene03835:CDS
AAGGTCCAGCCGAATAGGTG+TGG	0.567457	4.1:+44728110	MS.gene03835:CDS
TTTGGAGCAAAGATCTTGCA+TGG	0.576644	4.1:-44727883	None:intergenic
GTGCTGCTCCGGATACCTGT+GGG	0.602557	4.1:-44728198	None:intergenic
GTTCCTTCATCTGCAAATCA+CGG	0.602813	4.1:-44727911	None:intergenic
GGGACATGTAGACGGCGCGT+GGG	0.605951	4.1:+44728131	MS.gene03835:CDS
TATTCATCCATCTCCACCGT+CGG	0.610000	4.1:+44728016	MS.gene03835:CDS
AAGGCGAATCCAGTGGTGAA+GGG	0.610190	4.1:+44728266	MS.gene03835:CDS
ACAGGATTTAAGTGTCGGTG+CGG	0.618927	4.1:+44728158	MS.gene03835:CDS
TTCAGAGGCTGAACCACCGA+AGG	0.620244	4.1:+44728091	MS.gene03835:CDS
CTATTCGGCTGGACCTTCGG+TGG	0.620552	4.1:-44728104	None:intergenic
AGGTCCAGCCGAATAGGTGT+GGG	0.622585	4.1:+44728111	MS.gene03835:CDS
TAATTCTTCTTGTCTAGACA+CGG	0.623144	4.1:+44727989	MS.gene03835:CDS
GGTGCGGGTTGACGTTGTGT+GGG	0.623964	4.1:+44728174	MS.gene03835:CDS
ATAGGTGTGGGACATGTAGA+CGG	0.629117	4.1:+44728123	MS.gene03835:CDS
GCAGCTACCAACTCCACCGT+CGG	0.632431	4.1:-44728059	None:intergenic
GGACATGTAGACGGCGCGTG+GGG	0.632464	4.1:+44728132	MS.gene03835:CDS
AGTTTCTTCATCTACACCGA+CGG	0.637393	4.1:+44728043	MS.gene03835:CDS
TTCTTCATCTACACCGACGG+TGG	0.645268	4.1:+44728046	MS.gene03835:CDS
GGGAAGGGAGGAGATCAAGA+AGG	0.649649	4.1:+44728247	MS.gene03835:CDS
CACAGGTATCCGGAGCAGCA+CGG	0.653832	4.1:+44728200	MS.gene03835:CDS
GATCAAGAAGGCGAATCCAG+TGG	0.675101	4.1:+44728259	MS.gene03835:CDS
GATGAAGAAACTACAACCGA+CGG	0.679990	4.1:-44728032	None:intergenic
CGTCTCTGTGCTAACAACTG+CGG	0.696402	4.1:+44727843	MS.gene03835:CDS
GAAGAAACTACAACCGACGG+TGG	0.716652	4.1:-44728029	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TAATTCTTCTTGTCTAGACA+CGG	+	chr4.1:44727989-44728008	MS.gene03835:CDS	30.0%
	ACGTTCTTCATCTGCAAAAT+TGG	+	chr4.1:44727935-44727954	MS.gene03835:CDS	35.0%
	ACTGTTGAGAAATCAACGTT+TGG	-	chr4.1:44727968-44727987	None:intergenic	35.0%
!!	GGTTGTGGGTTTGATTTTAA+GGG	+	chr4.1:44728221-44728240	MS.gene03835:CDS	35.0%
!!	GTTGTGGGTTTGATTTTAAG+GGG	+	chr4.1:44728222-44728241	MS.gene03835:CDS	35.0%
	AGTTTCTTCATCTACACCGA+CGG	+	chr4.1:44728043-44728062	MS.gene03835:CDS	40.0%
	CTGCAAATCACGGTAACATT+TGG	-	chr4.1:44727904-44727923	None:intergenic	40.0%
	GATGAAGAAACTACAACCGA+CGG	-	chr4.1:44728035-44728054	None:intergenic	40.0%
	GTTCCTTCATCTGCAAATCA+CGG	-	chr4.1:44727914-44727933	None:intergenic	40.0%
!	TTACCGTGATTTGCAGATGA+AGG	+	chr4.1:44727908-44727927	MS.gene03835:CDS	40.0%
!	TTTGGAGCAAAGATCTTGCA+TGG	-	chr4.1:44727886-44727905	None:intergenic	40.0%
!!	CGGTTGTGGGTTTGATTTTA+AGG	+	chr4.1:44728220-44728239	MS.gene03835:CDS	40.0%
!!	GGGTTTGATTTTAAGGGGAT+GGG	+	chr4.1:44728227-44728246	MS.gene03835:CDS	40.0%
!!	TGATTTTAAGGGGATGGGAA+GGG	+	chr4.1:44728232-44728251	MS.gene03835:CDS	40.0%
!!	TGGGTTTGATTTTAAGGGGA+TGG	+	chr4.1:44728226-44728245	MS.gene03835:CDS	40.0%
!!	TTGATTTTAAGGGGATGGGA+AGG	+	chr4.1:44728231-44728250	MS.gene03835:CDS	40.0%
	ATAGGTGTGGGACATGTAGA+CGG	+	chr4.1:44728123-44728142	MS.gene03835:CDS	45.0%
	CTACATGTCCCACACCTATT+CGG	-	chr4.1:44728122-44728141	None:intergenic	45.0%
	GCTAACAACTGCGGTTTCTT+TGG	+	chr4.1:44727852-44727871	MS.gene03835:CDS	45.0%
	GGCTGACAGGATTTAAGTGT+CGG	+	chr4.1:44728153-44728172	MS.gene03835:CDS	45.0%
	TATTCATCCATCTCCACCGT+CGG	+	chr4.1:44728016-44728035	MS.gene03835:CDS	45.0%
!!	ACAGGATTTAAGTGTCGGTG+CGG	+	chr4.1:44728158-44728177	MS.gene03835:CDS	45.0%
	AAGGCGAATCCAGTGGTGAA+GGG	+	chr4.1:44728266-44728285	MS.gene03835:CDS	50.0%
	CGTCTCTGTGCTAACAACTG+CGG	+	chr4.1:44727843-44727862	MS.gene03835:CDS	50.0%
	GAAGAAACTACAACCGACGG+TGG	-	chr4.1:44728032-44728051	None:intergenic	50.0%
	GAGCAAAGATCTTGCATGGC+AGG	-	chr4.1:44727882-44727901	None:intergenic	50.0%
	GATCAAGAAGGCGAATCCAG+TGG	+	chr4.1:44728259-44728278	MS.gene03835:CDS	50.0%
	TTCTTCATCTACACCGACGG+TGG	+	chr4.1:44728046-44728065	MS.gene03835:CDS	50.0%
!!	CAGGATTTAAGTGTCGGTGC+GGG	+	chr4.1:44728159-44728178	MS.gene03835:CDS	50.0%
!!	TTTTAAGGGGATGGGAAGGG+AGG	+	chr4.1:44728235-44728254	MS.gene03835:CDS	50.0%
	AAGGTCCAGCCGAATAGGTG+TGG	+	chr4.1:44728110-44728129	MS.gene03835:CDS	55.0%
	ACTACAACCGACGGTGGAGA+TGG	-	chr4.1:44728026-44728045	None:intergenic	55.0%
	AGCTTCTCTCCCTTCACCAC+TGG	-	chr4.1:44728278-44728297	None:intergenic	55.0%
	AGGTCCAGCCGAATAGGTGT+GGG	+	chr4.1:44728111-44728130	MS.gene03835:CDS	55.0%
	ATCTACACCGACGGTGGAGT+TGG	+	chr4.1:44728052-44728071	MS.gene03835:CDS	55.0%
	ATGTCCCACACCTATTCGGC+TGG	-	chr4.1:44728118-44728137	None:intergenic	55.0%
	CACCTATTCGGCTGGACCTT+CGG	-	chr4.1:44728110-44728129	None:intergenic	55.0%
	GAAGGCGAATCCAGTGGTGA+AGG	+	chr4.1:44728265-44728284	MS.gene03835:CDS	55.0%
	GGGAAGGGAGGAGATCAAGA+AGG	+	chr4.1:44728247-44728266	MS.gene03835:CDS	55.0%
	TTCAGAGGCTGAACCACCGA+AGG	+	chr4.1:44728091-44728110	MS.gene03835:CDS	55.0%
	TTCGGTGGTTCAGCCTCTGA+AGG	-	chr4.1:44728092-44728111	None:intergenic	55.0%
	AGCTGCTGCTGGACCTTCAG+AGG	+	chr4.1:44728076-44728095	MS.gene03835:CDS	60.0%
	ATCCGGAGCAGCACGGTTGT+GGG	+	chr4.1:44728207-44728226	MS.gene03835:CDS	60.0%
	CACAGGTATCCGGAGCAGCA+CGG	+	chr4.1:44728200-44728219	MS.gene03835:CDS	60.0%
	CACCGAAGGTCCAGCCGAAT+AGG	+	chr4.1:44728105-44728124	MS.gene03835:CDS	60.0%
	CTATTCGGCTGGACCTTCGG+TGG	-	chr4.1:44728107-44728126	None:intergenic	60.0%
	GCAGCTACCAACTCCACCGT+CGG	-	chr4.1:44728062-44728081	None:intergenic	60.0%
	GTGTGGGACCCACAGGTATC+CGG	+	chr4.1:44728190-44728209	MS.gene03835:CDS	60.0%
	TATCCGGAGCAGCACGGTTG+TGG	+	chr4.1:44728206-44728225	MS.gene03835:CDS	60.0%
	TGACGTTGTGTGGGACCCAC+AGG	+	chr4.1:44728183-44728202	MS.gene03835:CDS	60.0%
!	AACCCACAACCGTGCTGCTC+CGG	-	chr4.1:44728212-44728231	None:intergenic	60.0%
!	GGTGCGGGTTGACGTTGTGT+GGG	+	chr4.1:44728174-44728193	MS.gene03835:CDS	60.0%
!	GTGCTGCTCCGGATACCTGT+GGG	-	chr4.1:44728201-44728220	None:intergenic	60.0%
!!	GTGGAGTTGGTAGCTGCTGC+TGG	+	chr4.1:44728065-44728084	MS.gene03835:CDS	60.0%
	GGACATGTAGACGGCGCGTG+GGG	+	chr4.1:44728132-44728151	MS.gene03835:CDS	65.0%
	GGGACATGTAGACGGCGCGT+GGG	+	chr4.1:44728131-44728150	MS.gene03835:CDS	65.0%
	TGGGACATGTAGACGGCGCG+TGG	+	chr4.1:44728130-44728149	MS.gene03835:CDS	65.0%
!	CGGTGCGGGTTGACGTTGTG+TGG	+	chr4.1:44728173-44728192	MS.gene03835:CDS	65.0%
!	CGTGCTGCTCCGGATACCTG+TGG	-	chr4.1:44728202-44728221	None:intergenic	65.0%
	AGACGGCGCGTGGGGCTGAC+AGG	+	chr4.1:44728140-44728159	MS.gene03835:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	44727810	44728310	44727810	ID=MS.gene03835
chr4.1	mRNA	44727810	44728310	44727810	ID=MS.gene03835.t1;Parent=MS.gene03835
chr4.1	exon	44727810	44728310	44727810	ID=MS.gene03835.t1.exon1;Parent=MS.gene03835.t1
chr4.1	CDS	44727810	44728310	44727810	ID=cds.MS.gene03835.t1;Parent=MS.gene03835.t1

Gene Sequence

>MS.gene03835

ATGGCTGAAGAACATAGATGTCAAGCTGCTCAACGTCTCTGTGCTAACAACTGCGGTTTCTTTGGAAGTCCTGCCATGCAAGATCTTTGCTCCAAATGTTACCGTGATTTGCAGATGAAGGAACAACGTTCTTCATCTGCAAAATTGGTTTTAAACCAAACGTTGATTTCTCAACAGTCTAATTCTTCTTGTCTAGACACGGAGATTATTCATCCATCTCCACCGTCGGTTGTAGTTTCTTCATCTACACCGACGGTGGAGTTGGTAGCTGCTGCTGGACCTTCAGAGGCTGAACCACCGAAGGTCCAGCCGAATAGGTGTGGGACATGTAGACGGCGCGTGGGGCTGACAGGATTTAAGTGTCGGTGCGGGTTGACGTTGTGTGGGACCCACAGGTATCCGGAGCAGCACGGTTGTGGGTTTGATTTTAAGGGGATGGGAAGGGAGGAGATCAAGAAGGCGAATCCAGTGGTGAAGGGAGAGAAGCTGAATAAGATCTGA

Protein sequence

>MS.gene03835.t1

MAEEHRCQAAQRLCANNCGFFGSPAMQDLCSKCYRDLQMKEQRSSSAKLVLNQTLISQQSNSSCLDTEIIHPSPPSVVVSSSTPTVELVAAAGPSEAEPPKVQPNRCGTCRRRVGLTGFKCRCGLTLCGTHRYPEQHGCGFDFKGMGREEIKKANPVVKGEKLNKI