Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24724.t1 | XP_003625793.1 | 85.9 | 170 | 22 | 1 | 1 | 170 | 1 | 168 | 1.70E-65 | 258.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24724.t1 | Q9SJM6 | 51.2 | 170 | 74 | 2 | 1 | 170 | 1 | 161 | 2.5e-41 | 169.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24724.t1 | G7L614 | 85.9 | 170 | 22 | 1 | 1 | 170 | 1 | 168 | 1.2e-65 | 258.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050335 | MS.gene24724 | 0.902204 | 1.23E-78 | -1.69E-46 |
| MS.gene05811 | MS.gene24724 | -0.806586 | 7.65E-50 | -1.69E-46 |
| MS.gene059974 | MS.gene24724 | -0.807785 | 4.27E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene24724 | MS.gene66771 | PPI |
| MS.gene003946 | MS.gene24724 | PPI |
| MS.gene03835 | MS.gene24724 | PPI |
| MS.gene24724 | MS.gene31086 | PPI |
| MS.gene24724 | MS.gene067716 | PPI |
| MS.gene052048 | MS.gene24724 | PPI |
| MS.gene055591 | MS.gene24724 | PPI |
| MS.gene78378 | MS.gene24724 | PPI |
| MS.gene46281 | MS.gene24724 | PPI |
| MS.gene45557 | MS.gene24724 | PPI |
| MS.gene97659 | MS.gene24724 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24724.t1 | MTR_7g104320 | 97.647 | 170 | 2 | 1 | 1 | 170 | 1 | 168 | 8.27e-121 | 338 |
| MS.gene24724.t1 | MTR_1g060380 | 58.824 | 170 | 56 | 3 | 1 | 170 | 1 | 156 | 5.69e-60 | 183 |
| MS.gene24724.t1 | MTR_4g053440 | 53.672 | 177 | 68 | 5 | 1 | 170 | 1 | 170 | 3.65e-59 | 182 |
| MS.gene24724.t1 | MTR_2g086190 | 51.765 | 170 | 62 | 5 | 1 | 170 | 1 | 150 | 1.26e-54 | 170 |
| MS.gene24724.t1 | MTR_0249s0070 | 44.706 | 170 | 87 | 3 | 3 | 170 | 5 | 169 | 3.55e-47 | 151 |
| MS.gene24724.t1 | MTR_0249s0070 | 44.706 | 170 | 87 | 3 | 3 | 170 | 13 | 177 | 4.24e-47 | 151 |
| MS.gene24724.t1 | MTR_2g098160 | 44.910 | 167 | 80 | 4 | 10 | 170 | 12 | 172 | 4.61e-46 | 149 |
| MS.gene24724.t1 | MTR_7g092400 | 43.353 | 173 | 88 | 2 | 3 | 170 | 93 | 260 | 1.82e-45 | 150 |
| MS.gene24724.t1 | MTR_4g065570 | 38.028 | 142 | 83 | 1 | 29 | 170 | 1 | 137 | 7.16e-33 | 114 |
| MS.gene24724.t1 | MTR_7g114920 | 39.610 | 154 | 66 | 4 | 15 | 168 | 5 | 131 | 1.61e-30 | 108 |
| MS.gene24724.t1 | MTR_1g100773 | 37.908 | 153 | 75 | 5 | 15 | 167 | 5 | 137 | 3.18e-29 | 105 |
| MS.gene24724.t1 | MTR_3g028010 | 33.742 | 163 | 74 | 3 | 15 | 170 | 28 | 163 | 2.23e-26 | 98.6 |
| MS.gene24724.t1 | MTR_8g036980 | 59.677 | 62 | 25 | 0 | 109 | 170 | 90 | 151 | 1.01e-24 | 94.0 |
| MS.gene24724.t1 | MTR_2g054650 | 37.255 | 153 | 62 | 4 | 15 | 167 | 6 | 124 | 3.55e-24 | 91.7 |
| MS.gene24724.t1 | MTR_3g025570 | 60.000 | 40 | 16 | 0 | 109 | 148 | 8 | 47 | 1.15e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24724.t1 | AT2G36320 | 59.412 | 170 | 60 | 3 | 1 | 170 | 1 | 161 | 2.00e-65 | 197 |
| MS.gene24724.t1 | AT3G52800 | 53.757 | 173 | 74 | 3 | 1 | 170 | 1 | 170 | 6.97e-52 | 163 |
| MS.gene24724.t1 | AT3G52800 | 53.757 | 173 | 74 | 3 | 1 | 170 | 1 | 170 | 6.97e-52 | 163 |
| MS.gene24724.t1 | AT2G27580 | 50.292 | 171 | 76 | 4 | 1 | 170 | 1 | 163 | 1.34e-49 | 157 |
| MS.gene24724.t1 | AT2G27580 | 50.292 | 171 | 76 | 4 | 1 | 170 | 1 | 163 | 1.34e-49 | 157 |
| MS.gene24724.t1 | AT4G22820 | 49.080 | 163 | 71 | 4 | 14 | 170 | 20 | 176 | 2.30e-49 | 157 |
| MS.gene24724.t1 | AT4G22820 | 49.080 | 163 | 71 | 4 | 14 | 170 | 20 | 176 | 2.30e-49 | 157 |
| MS.gene24724.t1 | AT4G12040 | 47.619 | 168 | 76 | 4 | 10 | 170 | 13 | 175 | 4.46e-47 | 151 |
| MS.gene24724.t1 | AT4G12040 | 47.619 | 168 | 76 | 4 | 10 | 170 | 13 | 175 | 4.46e-47 | 151 |
| MS.gene24724.t1 | AT1G12440 | 44.706 | 170 | 73 | 3 | 9 | 170 | 12 | 168 | 8.03e-43 | 140 |
| MS.gene24724.t1 | AT1G12440 | 44.706 | 170 | 73 | 3 | 9 | 170 | 12 | 168 | 8.03e-43 | 140 |
| MS.gene24724.t1 | AT1G12440 | 44.706 | 170 | 73 | 3 | 9 | 170 | 12 | 168 | 8.03e-43 | 140 |
| MS.gene24724.t1 | AT1G51200 | 46.707 | 167 | 78 | 3 | 10 | 170 | 12 | 173 | 2.96e-40 | 134 |
| MS.gene24724.t1 | AT1G51200 | 46.707 | 167 | 78 | 3 | 10 | 170 | 12 | 173 | 2.96e-40 | 134 |
| MS.gene24724.t1 | AT1G51200 | 46.707 | 167 | 78 | 3 | 10 | 170 | 12 | 173 | 2.96e-40 | 134 |
| MS.gene24724.t1 | AT1G51200 | 46.707 | 167 | 78 | 3 | 10 | 170 | 12 | 173 | 2.96e-40 | 134 |
| MS.gene24724.t1 | AT4G25380 | 36.129 | 155 | 65 | 4 | 16 | 170 | 10 | 130 | 2.12e-27 | 100 |
| MS.gene24724.t1 | AT3G12630 | 34.211 | 152 | 76 | 4 | 21 | 170 | 31 | 160 | 1.75e-24 | 93.6 |
| MS.gene24724.t1 | AT4G14225 | 35.032 | 157 | 63 | 4 | 15 | 170 | 7 | 125 | 4.87e-23 | 89.0 |
Find 52 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGCAGAGTCTGTGGCCTTC+TGG | 0.265748 | 7.4:-7327504 | None:intergenic |
| CTTGTTCTTGTTCCTTCAAA+TGG | 0.292468 | 7.4:-7327593 | None:intergenic |
| TGTGAATTGTCCGATGCTTC+AGG | 0.360056 | 7.4:-7327762 | None:intergenic |
| AACGCCGGTGGTTGAGGTTG+AGG | 0.369798 | 7.4:-7327720 | None:intergenic |
| AGAAAGAGGACCGGGTTAAC+CGG | 0.386243 | 7.4:+7327819 | MS.gene24724:CDS |
| GGAGATAGCGAAAGCGAATC+CGG | 0.416264 | 7.4:+7327935 | MS.gene24724:CDS |
| GCAAACAACTGCGGCTTCTT+CGG | 0.427864 | 7.4:+7327525 | MS.gene24724:CDS |
| TTGTGGGTCCCACAGGTATC+CGG | 0.444625 | 7.4:+7327869 | MS.gene24724:CDS |
| AGAGATTCGATTTCCGCAAC+GGG | 0.444842 | 7.4:-7327696 | None:intergenic |
| AGCTTATCAGCTTTGATCAC+CGG | 0.447035 | 7.4:-7327954 | None:intergenic |
| AACGAACACGGCGGTGGAAG+CGG | 0.470759 | 7.4:-7327664 | None:intergenic |
| CGGTTCAATCGAACCGGTGC+GGG | 0.475968 | 7.4:+7327790 | MS.gene24724:CDS |
| AACTAAATCAACAATCGAAA+CGG | 0.478568 | 7.4:+7327620 | MS.gene24724:CDS |
| ACGGTCAACGCCGGTGGTTG+AGG | 0.481127 | 7.4:-7327726 | None:intergenic |
| AGCAGCAACGAACACGGCGG+TGG | 0.486244 | 7.4:-7327670 | None:intergenic |
| CCGCACCGGTTCGATTGAAC+CGG | 0.490138 | 7.4:-7327789 | None:intergenic |
| TTACAGAGACATCCATTTGA+AGG | 0.490147 | 7.4:+7327581 | MS.gene24724:CDS |
| ACGCCGGTGGTTGAGGTTGA+GGG | 0.510233 | 7.4:-7327719 | None:intergenic |
| CGTTGCTGCTTCGCCCGTTG+CGG | 0.514245 | 7.4:+7327683 | MS.gene24724:CDS |
| ATCGGACAATTCACAAGGAC+CGG | 0.522802 | 7.4:+7327770 | MS.gene24724:CDS |
| GGTGGAAGCGGATGCTGAGG+CGG | 0.523382 | 7.4:-7327652 | None:intergenic |
| AAACCGCACTCATGTTTCTC+CGG | 0.524064 | 7.4:-7327888 | None:intergenic |
| TTTCAAAGCAGTGGGGAGAG+AGG | 0.529533 | 7.4:+7327914 | MS.gene24724:CDS |
| TCTTTCTACACGTCCCGCAC+CGG | 0.534045 | 7.4:-7327803 | None:intergenic |
| GAAAGAGGACCGGGTTAACC+GGG | 0.537492 | 7.4:+7327820 | MS.gene24724:CDS |
| AAGGACCGGTTCAATCGAAC+CGG | 0.537575 | 7.4:+7327784 | MS.gene24724:CDS |
| GGAAGCGGATGCTGAGGCGG+AGG | 0.542625 | 7.4:-7327649 | None:intergenic |
| GTCGATCGTGCCTGAAGCAT+CGG | 0.545884 | 7.4:+7327752 | MS.gene24724:CDS |
| AGTTGTTTGCGCAGAGTCTG+TGG | 0.555837 | 7.4:-7327512 | None:intergenic |
| GGGCGAAGCAGCAACGAACA+CGG | 0.557556 | 7.4:-7327676 | None:intergenic |
| ACACTTGAACCCGGTTAACC+CGG | 0.561755 | 7.4:-7327829 | None:intergenic |
| GGCGGTGGAAGCGGATGCTG+AGG | 0.566120 | 7.4:-7327655 | None:intergenic |
| GAGAGATTCGATTTCCGCAA+CGG | 0.574585 | 7.4:-7327697 | None:intergenic |
| CATGTTTCTCCGGATACCTG+TGG | 0.575785 | 7.4:-7327878 | None:intergenic |
| GGTGCGGGACGTGTAGAAAG+AGG | 0.587813 | 7.4:+7327805 | MS.gene24724:CDS |
| ACCGGGTTCAAGTGTAGATG+TGG | 0.588078 | 7.4:+7327837 | MS.gene24724:CDS |
| GACGGTACGGTCAACGCCGG+TGG | 0.590519 | 7.4:-7327732 | None:intergenic |
| TCCACATCTACACTTGAACC+CGG | 0.592518 | 7.4:-7327838 | None:intergenic |
| GGGACGTGTAGAAAGAGGAC+CGG | 0.594127 | 7.4:+7327810 | MS.gene24724:CDS |
| GGCCTTCTGGAGTTTCACAT+CGG | 0.606780 | 7.4:-7327491 | None:intergenic |
| GGACGTGTAGAAAGAGGACC+GGG | 0.606988 | 7.4:+7327811 | MS.gene24724:CDS |
| ATGTTTCTCCGGATACCTGT+GGG | 0.618435 | 7.4:-7327877 | None:intergenic |
| TTCAGGCACGATCGACGGTA+CGG | 0.620849 | 7.4:-7327745 | None:intergenic |
| TCTCCCTCAACCTCAACCAC+CGG | 0.644565 | 7.4:+7327716 | MS.gene24724:CDS |
| GATGCTTCAGGCACGATCGA+CGG | 0.657260 | 7.4:-7327750 | None:intergenic |
| ATCGACGGTACGGTCAACGC+CGG | 0.667919 | 7.4:-7327735 | None:intergenic |
| CCGGTTCAATCGAACCGGTG+CGG | 0.668012 | 7.4:+7327789 | MS.gene24724:CDS |
| CACCGATGTGAAACTCCAGA+AGG | 0.686088 | 7.4:+7327489 | MS.gene24724:CDS |
| GAAGCATCGGACAATTCACA+AGG | 0.714221 | 7.4:+7327765 | MS.gene24724:CDS |
| AGACTCTGCGCAAACAACTG+CGG | 0.735745 | 7.4:+7327516 | MS.gene24724:CDS |
| TATCCGGAGAAACATGAGTG+CGG | 0.743977 | 7.4:+7327885 | MS.gene24724:CDS |
| CGAAGCAGCAACGAACACGG+CGG | 0.748812 | 7.4:-7327673 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTAAATCAACAATCGAAA+CGG | + | chr7.4:7327620-7327639 | MS.gene24724:CDS | 25.0% |
| CTTGTTCTTGTTCCTTCAAA+TGG | - | chr7.4:7327596-7327615 | None:intergenic | 35.0% | |
| TTACAGAGACATCCATTTGA+AGG | + | chr7.4:7327581-7327600 | MS.gene24724:CDS | 35.0% | |
| ! | AGATGTGGAATAACGTTTTG+TGG | + | chr7.4:7327852-7327871 | MS.gene24724:CDS | 35.0% |
| ! | GATGTGGAATAACGTTTTGT+GGG | + | chr7.4:7327853-7327872 | MS.gene24724:CDS | 35.0% |
| !!! | GGTTTTGATTTCAAAGCAGT+GGG | + | chr7.4:7327906-7327925 | MS.gene24724:CDS | 35.0% |
| !!! | GTTTTGATTTCAAAGCAGTG+GGG | + | chr7.4:7327907-7327926 | MS.gene24724:CDS | 35.0% |
| ! | AGCTTATCAGCTTTGATCAC+CGG | - | chr7.4:7327957-7327976 | None:intergenic | 40.0% |
| !!! | CGGTTTTGATTTCAAAGCAG+TGG | + | chr7.4:7327905-7327924 | MS.gene24724:CDS | 40.0% |
| AAACCGCACTCATGTTTCTC+CGG | - | chr7.4:7327891-7327910 | None:intergenic | 45.0% | |
| AGAGATTCGATTTCCGCAAC+GGG | - | chr7.4:7327699-7327718 | None:intergenic | 45.0% | |
| ATCGGACAATTCACAAGGAC+CGG | + | chr7.4:7327770-7327789 | MS.gene24724:CDS | 45.0% | |
| ATGTTTCTCCGGATACCTGT+GGG | - | chr7.4:7327880-7327899 | None:intergenic | 45.0% | |
| GAGAGATTCGATTTCCGCAA+CGG | - | chr7.4:7327700-7327719 | None:intergenic | 45.0% | |
| TATCCGGAGAAACATGAGTG+CGG | + | chr7.4:7327885-7327904 | MS.gene24724:CDS | 45.0% | |
| TCCACATCTACACTTGAACC+CGG | - | chr7.4:7327841-7327860 | None:intergenic | 45.0% | |
| TGTGAATTGTCCGATGCTTC+AGG | - | chr7.4:7327765-7327784 | None:intergenic | 45.0% | |
| !! | GAAGCATCGGACAATTCACA+AGG | + | chr7.4:7327765-7327784 | MS.gene24724:CDS | 45.0% |
| ACACTTGAACCCGGTTAACC+CGG | - | chr7.4:7327832-7327851 | None:intergenic | 50.0% | |
| ACCGGGTTCAAGTGTAGATG+TGG | + | chr7.4:7327837-7327856 | MS.gene24724:CDS | 50.0% | |
| AGAAAGAGGACCGGGTTAAC+CGG | + | chr7.4:7327819-7327838 | MS.gene24724:CDS | 50.0% | |
| AGACTCTGCGCAAACAACTG+CGG | + | chr7.4:7327516-7327535 | MS.gene24724:CDS | 50.0% | |
| AGTTGTTTGCGCAGAGTCTG+TGG | - | chr7.4:7327515-7327534 | None:intergenic | 50.0% | |
| CACCGATGTGAAACTCCAGA+AGG | + | chr7.4:7327489-7327508 | MS.gene24724:CDS | 50.0% | |
| CATGTTTCTCCGGATACCTG+TGG | - | chr7.4:7327881-7327900 | None:intergenic | 50.0% | |
| GCAAACAACTGCGGCTTCTT+CGG | + | chr7.4:7327525-7327544 | MS.gene24724:CDS | 50.0% | |
| GGAGATAGCGAAAGCGAATC+CGG | + | chr7.4:7327935-7327954 | MS.gene24724:CDS | 50.0% | |
| TTTCAAAGCAGTGGGGAGAG+AGG | + | chr7.4:7327914-7327933 | MS.gene24724:CDS | 50.0% | |
| ! | AAGGACCGGTTCAATCGAAC+CGG | + | chr7.4:7327784-7327803 | MS.gene24724:CDS | 50.0% |
| ! | TAACGTTTTGTGGGTCCCAC+AGG | + | chr7.4:7327862-7327881 | MS.gene24724:CDS | 50.0% |
| !! | GGCCTTCTGGAGTTTCACAT+CGG | - | chr7.4:7327494-7327513 | None:intergenic | 50.0% |
| GAAAGAGGACCGGGTTAACC+GGG | + | chr7.4:7327820-7327839 | MS.gene24724:CDS | 55.0% | |
| GATGCTTCAGGCACGATCGA+CGG | - | chr7.4:7327753-7327772 | None:intergenic | 55.0% | |
| GGACGTGTAGAAAGAGGACC+GGG | + | chr7.4:7327811-7327830 | MS.gene24724:CDS | 55.0% | |
| GGGACGTGTAGAAAGAGGAC+CGG | + | chr7.4:7327810-7327829 | MS.gene24724:CDS | 55.0% | |
| GTCGATCGTGCCTGAAGCAT+CGG | + | chr7.4:7327752-7327771 | MS.gene24724:CDS | 55.0% | |
| TCTCCCTCAACCTCAACCAC+CGG | + | chr7.4:7327716-7327735 | MS.gene24724:CDS | 55.0% | |
| TCTTTCTACACGTCCCGCAC+CGG | - | chr7.4:7327806-7327825 | None:intergenic | 55.0% | |
| TTCAGGCACGATCGACGGTA+CGG | - | chr7.4:7327748-7327767 | None:intergenic | 55.0% | |
| TTGTGGGTCCCACAGGTATC+CGG | + | chr7.4:7327869-7327888 | MS.gene24724:CDS | 55.0% | |
| AACGAACACGGCGGTGGAAG+CGG | - | chr7.4:7327667-7327686 | None:intergenic | 60.0% | |
| AACGCCGGTGGTTGAGGTTG+AGG | - | chr7.4:7327723-7327742 | None:intergenic | 60.0% | |
| ACGCCGGTGGTTGAGGTTGA+GGG | - | chr7.4:7327722-7327741 | None:intergenic | 60.0% | |
| ATCGACGGTACGGTCAACGC+CGG | - | chr7.4:7327738-7327757 | None:intergenic | 60.0% | |
| CCGGTTCAATCGAACCGGTG+CGG | + | chr7.4:7327789-7327808 | MS.gene24724:CDS | 60.0% | |
| CGAAGCAGCAACGAACACGG+CGG | - | chr7.4:7327676-7327695 | None:intergenic | 60.0% | |
| CGGTTCAATCGAACCGGTGC+GGG | + | chr7.4:7327790-7327809 | MS.gene24724:CDS | 60.0% | |
| GGGCGAAGCAGCAACGAACA+CGG | - | chr7.4:7327679-7327698 | None:intergenic | 60.0% | |
| GGTGCGGGACGTGTAGAAAG+AGG | + | chr7.4:7327805-7327824 | MS.gene24724:CDS | 60.0% | |
| !! | CCGCACCGGTTCGATTGAAC+CGG | - | chr7.4:7327792-7327811 | None:intergenic | 60.0% |
| ACGGTCAACGCCGGTGGTTG+AGG | - | chr7.4:7327729-7327748 | None:intergenic | 65.0% | |
| AGCAGCAACGAACACGGCGG+TGG | - | chr7.4:7327673-7327692 | None:intergenic | 65.0% | |
| GCGCAGAGTCTGTGGCCTTC+TGG | - | chr7.4:7327507-7327526 | None:intergenic | 65.0% | |
| GGTGGAAGCGGATGCTGAGG+CGG | - | chr7.4:7327655-7327674 | None:intergenic | 65.0% | |
| ! | CGTTGCTGCTTCGCCCGTTG+CGG | + | chr7.4:7327683-7327702 | MS.gene24724:CDS | 65.0% |
| GACGGTACGGTCAACGCCGG+TGG | - | chr7.4:7327735-7327754 | None:intergenic | 70.0% | |
| GGAAGCGGATGCTGAGGCGG+AGG | - | chr7.4:7327652-7327671 | None:intergenic | 70.0% | |
| GGCGGTGGAAGCGGATGCTG+AGG | - | chr7.4:7327658-7327677 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 7327477 | 7327989 | 7327477 | ID=MS.gene24724 |
| chr7.4 | mRNA | 7327477 | 7327989 | 7327477 | ID=MS.gene24724.t1;Parent=MS.gene24724 |
| chr7.4 | exon | 7327477 | 7327989 | 7327477 | ID=MS.gene24724.t1.exon1;Parent=MS.gene24724.t1 |
| chr7.4 | CDS | 7327477 | 7327989 | 7327477 | ID=cds.MS.gene24724.t1;Parent=MS.gene24724.t1 |
| Gene Sequence |
| Protein sequence |
