AlfalfaGEDB | MS.gene050335

M. sativa cultivar XinJiangDaYe / MS.gene050335

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF01428.19 PF01754.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050335.t1	XP_003625793.1	84.1	170	25	1	1	170	1	168	2.70E-63	251.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050335.t1	Q9SJM6	51.2	170	74	2	1	170	1	161	1.9e-41	170.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050335.t1	G7L614	84.1	170	25	1	1	170	1	168	2.0e-63	251.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050335	MS.gene056796	-0.816973	4.20E-52	-1.69E-46
MS.gene050335	MS.gene20098	-0.803709	3.06E-49	-1.69E-46
MS.gene050335	MS.gene24724	0.902204	1.23E-78	-1.69E-46
MS.gene050335	MS.gene29515	-0.813161	2.94E-51	-1.69E-46
MS.gene050335	MS.gene50134	-0.801255	9.81E-49	-1.69E-46
MS.gene050335	MS.gene59218	0.805481	1.31E-49	-1.69E-46
MS.gene050335	MS.gene76043	0.814075	1.85E-51	-1.69E-46
MS.gene050335	MS.gene98214	-0.812733	3.65E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene050335	MS.gene46281	PPI
MS.gene97659	MS.gene050335	PPI
MS.gene050335	MS.gene052048	PPI
MS.gene050335	MS.gene66771	PPI
MS.gene050335	MS.gene31086	PPI
MS.gene050335	MS.gene067716	PPI
MS.gene050335	MS.gene03835	PPI
MS.gene050335	MS.gene45557	PPI
MS.gene78378	MS.gene050335	PPI
MS.gene050335	MS.gene055591	PPI
MS.gene050335	MS.gene003946	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050335.t1	MTR_7g104320	97.059	170	3	1	1	170	1	168	8.75e-120	335
MS.gene050335.t1	MTR_1g060380	58.824	170	56	3	1	170	1	156	4.63e-60	184
MS.gene050335.t1	MTR_4g053440	53.672	177	68	5	1	170	1	170	2.78e-59	182
MS.gene050335.t1	MTR_2g086190	50.000	170	65	4	1	170	1	150	1.25e-53	167
MS.gene050335.t1	MTR_0249s0070	43.931	173	84	4	3	170	5	169	3.19e-47	151
MS.gene050335.t1	MTR_0249s0070	44.706	170	87	3	3	170	13	177	4.83e-47	151
MS.gene050335.t1	MTR_2g098160	44.910	167	80	4	10	170	12	172	1.18e-46	150
MS.gene050335.t1	MTR_7g092400	43.353	173	88	2	3	170	93	260	7.41e-46	151
MS.gene050335.t1	MTR_4g065570	39.583	144	78	3	29	170	1	137	2.88e-33	115
MS.gene050335.t1	MTR_7g114920	39.610	154	66	4	15	168	5	131	1.41e-30	108
MS.gene050335.t1	MTR_1g100773	37.908	153	75	5	15	167	5	137	1.99e-29	105
MS.gene050335.t1	MTR_3g028010	33.742	163	74	3	15	170	28	163	2.52e-26	98.2
MS.gene050335.t1	MTR_8g036980	59.677	62	25	0	109	170	90	151	1.03e-24	94.0
MS.gene050335.t1	MTR_2g054650	37.255	153	62	4	15	167	6	124	3.05e-24	92.0
MS.gene050335.t1	MTR_3g025570	60.000	40	16	0	109	148	8	47	1.13e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050335.t1	AT2G36320	59.412	170	60	3	1	170	1	161	1.24e-65	198
MS.gene050335.t1	AT3G52800	53.757	173	74	3	1	170	1	170	2.58e-52	164
MS.gene050335.t1	AT3G52800	53.757	173	74	3	1	170	1	170	2.58e-52	164
MS.gene050335.t1	AT2G27580	48.824	170	80	2	1	170	1	163	6.53e-50	158
MS.gene050335.t1	AT2G27580	48.824	170	80	2	1	170	1	163	6.53e-50	158
MS.gene050335.t1	AT4G22820	49.080	163	71	4	14	170	20	176	1.01e-49	158
MS.gene050335.t1	AT4G22820	49.080	163	71	4	14	170	20	176	1.01e-49	158
MS.gene050335.t1	AT4G12040	47.619	168	76	4	10	170	13	175	1.60e-47	152
MS.gene050335.t1	AT4G12040	47.619	168	76	4	10	170	13	175	1.60e-47	152
MS.gene050335.t1	AT1G12440	44.512	164	82	2	9	170	12	168	5.49e-43	141
MS.gene050335.t1	AT1G12440	44.512	164	82	2	9	170	12	168	5.49e-43	141
MS.gene050335.t1	AT1G12440	44.512	164	82	2	9	170	12	168	5.49e-43	141
MS.gene050335.t1	AT1G51200	46.707	167	78	3	10	170	12	173	2.49e-40	134
MS.gene050335.t1	AT1G51200	46.707	167	78	3	10	170	12	173	2.49e-40	134
MS.gene050335.t1	AT1G51200	46.707	167	78	3	10	170	12	173	2.49e-40	134
MS.gene050335.t1	AT1G51200	46.707	167	78	3	10	170	12	173	2.49e-40	134
MS.gene050335.t1	AT4G25380	36.129	155	65	4	16	170	10	130	1.39e-27	100
MS.gene050335.t1	AT3G12630	34.211	152	76	4	21	170	31	160	9.89e-25	94.4
MS.gene050335.t1	AT4G14225	38.462	156	59	6	15	170	7	125	4.01e-23	89.0

Find 51 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCGCAGAGTCTGTGGCCTTC+TGG	0.265748	7.2:+8447839	None:intergenic
CTTGTTCTTGTTCCTTCAAA+TGG	0.292468	7.2:+8447750	None:intergenic
TGTGAATTGTCCGATGCTTC+AGG	0.360056	7.2:+8447581	None:intergenic
AACGCCTGTGGTTGAGGTTG+AGG	0.384729	7.2:+8447623	None:intergenic
AGAAAGAGGACCGGGTTAAC+CGG	0.386243	7.2:-8447524	MS.gene050335:CDS
GGAGATAGCGAAAGCGAATC+CGG	0.416264	7.2:-8447408	MS.gene050335:CDS
GCAAACAACTGCGGCTTCTT+CGG	0.427864	7.2:-8447818	MS.gene050335:CDS
TTGTGGGTCCCACAGGTATC+CGG	0.444625	7.2:-8447474	MS.gene050335:CDS
AGAGATTCGATTTCCGCAAC+GGG	0.444842	7.2:+8447647	None:intergenic
AGCTTATCAGCTTTGATCAC+CGG	0.447035	7.2:+8447389	None:intergenic
AACGAACACGGCGGTGGAAG+CGG	0.470759	7.2:+8447679	None:intergenic
CGGTTCAATCGAACCGGTGC+GGG	0.475968	7.2:-8447553	MS.gene050335:CDS
AACTAAATCAACAATCGAAA+CGG	0.478568	7.2:-8447723	MS.gene050335:CDS
AGCAGCAACGAACACGGCGG+TGG	0.486244	7.2:+8447673	None:intergenic
CCGCACCGGTTCGATTGAAC+CGG	0.490138	7.2:+8447554	None:intergenic
TTACAGAGACATCCATTTGA+AGG	0.490147	7.2:-8447762	MS.gene050335:CDS
ACGGTCAACGCCTGTGGTTG+AGG	0.504756	7.2:+8447617	None:intergenic
ACGCCTGTGGTTGAGGTTGA+GGG	0.505809	7.2:+8447624	None:intergenic
CGTTGCTGCTTCGCCCGTTG+CGG	0.514245	7.2:-8447660	MS.gene050335:CDS
ATCGGACAATTCACAAGGAC+CGG	0.522802	7.2:-8447573	MS.gene050335:CDS
GGTGGAAGCGGATGCTGAGG+CGG	0.523382	7.2:+8447691	None:intergenic
AAACCGCACTCATGTTTCTC+CGG	0.524064	7.2:+8447455	None:intergenic
TTTCAAAGCAGTGGGGAGAG+AGG	0.529533	7.2:-8447429	MS.gene050335:CDS
TCTTTCTACACGTCCCGCAC+CGG	0.534045	7.2:+8447540	None:intergenic
GAAAGAGGACCGGGTTAACC+GGG	0.537492	7.2:-8447523	MS.gene050335:CDS
AAGGACCGGTTCAATCGAAC+CGG	0.537575	7.2:-8447559	MS.gene050335:CDS
GGAAGCGGATGCTGAGGCGG+AGG	0.542625	7.2:+8447694	None:intergenic
GTCGATCGTGCCTGAAGCAT+CGG	0.545884	7.2:-8447591	MS.gene050335:CDS
AGTTGTTTGCGCAGAGTCTG+TGG	0.555837	7.2:+8447831	None:intergenic
GGGCGAAGCAGCAACGAACA+CGG	0.557556	7.2:+8447667	None:intergenic
ACACTTGAACCCGGTTAACC+CGG	0.561755	7.2:+8447514	None:intergenic
GGCGGTGGAAGCGGATGCTG+AGG	0.566120	7.2:+8447688	None:intergenic
GAGAGATTCGATTTCCGCAA+CGG	0.574585	7.2:+8447646	None:intergenic
CATGTTTCTCCGGATACCTG+TGG	0.575785	7.2:+8447465	None:intergenic
TCTCCCTCAACCTCAACCAC+AGG	0.587611	7.2:-8447627	MS.gene050335:CDS
GGTGCGGGACGTGTAGAAAG+AGG	0.587813	7.2:-8447538	MS.gene050335:CDS
ACCGGGTTCAAGTGTAGATG+TGG	0.588078	7.2:-8447506	MS.gene050335:CDS
TCCACATCTACACTTGAACC+CGG	0.592518	7.2:+8447505	None:intergenic
GGGACGTGTAGAAAGAGGAC+CGG	0.594127	7.2:-8447533	MS.gene050335:CDS
GACGGTACGGTCAACGCCTG+TGG	0.595261	7.2:+8447611	None:intergenic
GGCCTTCTGGAGTTTCACAT+CGG	0.606780	7.2:+8447852	None:intergenic
GGACGTGTAGAAAGAGGACC+GGG	0.606988	7.2:-8447532	MS.gene050335:CDS
ATGTTTCTCCGGATACCTGT+GGG	0.618435	7.2:+8447466	None:intergenic
TTCAGGCACGATCGACGGTA+CGG	0.620849	7.2:+8447598	None:intergenic
GATGCTTCAGGCACGATCGA+CGG	0.657260	7.2:+8447593	None:intergenic
CCGGTTCAATCGAACCGGTG+CGG	0.668012	7.2:-8447554	MS.gene050335:CDS
CACCGATGTGAAACTCCAGA+AGG	0.686088	7.2:-8447854	MS.gene050335:CDS
GAAGCATCGGACAATTCACA+AGG	0.714221	7.2:-8447578	MS.gene050335:CDS
AGACTCTGCGCAAACAACTG+CGG	0.735745	7.2:-8447827	MS.gene050335:CDS
TATCCGGAGAAACATGAGTG+CGG	0.743977	7.2:-8447458	MS.gene050335:CDS
CGAAGCAGCAACGAACACGG+CGG	0.748812	7.2:+8447670	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AACTAAATCAACAATCGAAA+CGG	-	chr7.2:8447519-8447538	MS.gene050335:CDS	25.0%
	CTTGTTCTTGTTCCTTCAAA+TGG	+	chr7.2:8447495-8447514	None:intergenic	35.0%
	TTACAGAGACATCCATTTGA+AGG	-	chr7.2:8447480-8447499	MS.gene050335:CDS	35.0%
!	AGATGTGGAATAACGTTTTG+TGG	-	chr7.2:8447751-8447770	MS.gene050335:CDS	35.0%
!	GATGTGGAATAACGTTTTGT+GGG	-	chr7.2:8447752-8447771	MS.gene050335:CDS	35.0%
!!!	GGTTTTGATTTCAAAGCAGT+GGG	-	chr7.2:8447805-8447824	MS.gene050335:CDS	35.0%
!!!	GTTTTGATTTCAAAGCAGTG+GGG	-	chr7.2:8447806-8447825	MS.gene050335:CDS	35.0%
!	AGCTTATCAGCTTTGATCAC+CGG	+	chr7.2:8447856-8447875	None:intergenic	40.0%
!!!	CGGTTTTGATTTCAAAGCAG+TGG	-	chr7.2:8447804-8447823	MS.gene050335:CDS	40.0%
	AAACCGCACTCATGTTTCTC+CGG	+	chr7.2:8447790-8447809	None:intergenic	45.0%
	AGAGATTCGATTTCCGCAAC+GGG	+	chr7.2:8447598-8447617	None:intergenic	45.0%
	ATCGGACAATTCACAAGGAC+CGG	-	chr7.2:8447669-8447688	MS.gene050335:CDS	45.0%
	ATGTTTCTCCGGATACCTGT+GGG	+	chr7.2:8447779-8447798	None:intergenic	45.0%
	GAGAGATTCGATTTCCGCAA+CGG	+	chr7.2:8447599-8447618	None:intergenic	45.0%
	TATCCGGAGAAACATGAGTG+CGG	-	chr7.2:8447784-8447803	MS.gene050335:CDS	45.0%
	TCCACATCTACACTTGAACC+CGG	+	chr7.2:8447740-8447759	None:intergenic	45.0%
	TGTGAATTGTCCGATGCTTC+AGG	+	chr7.2:8447664-8447683	None:intergenic	45.0%
!!	GAAGCATCGGACAATTCACA+AGG	-	chr7.2:8447664-8447683	MS.gene050335:CDS	45.0%
	ACACTTGAACCCGGTTAACC+CGG	+	chr7.2:8447731-8447750	None:intergenic	50.0%
	ACCGGGTTCAAGTGTAGATG+TGG	-	chr7.2:8447736-8447755	MS.gene050335:CDS	50.0%
	AGAAAGAGGACCGGGTTAAC+CGG	-	chr7.2:8447718-8447737	MS.gene050335:CDS	50.0%
	AGACTCTGCGCAAACAACTG+CGG	-	chr7.2:8447415-8447434	MS.gene050335:CDS	50.0%
	AGTTGTTTGCGCAGAGTCTG+TGG	+	chr7.2:8447414-8447433	None:intergenic	50.0%
	CACCGATGTGAAACTCCAGA+AGG	-	chr7.2:8447388-8447407	MS.gene050335:CDS	50.0%
	CATGTTTCTCCGGATACCTG+TGG	+	chr7.2:8447780-8447799	None:intergenic	50.0%
	GCAAACAACTGCGGCTTCTT+CGG	-	chr7.2:8447424-8447443	MS.gene050335:CDS	50.0%
	GGAGATAGCGAAAGCGAATC+CGG	-	chr7.2:8447834-8447853	MS.gene050335:CDS	50.0%
	TTTCAAAGCAGTGGGGAGAG+AGG	-	chr7.2:8447813-8447832	MS.gene050335:CDS	50.0%
!	AAGGACCGGTTCAATCGAAC+CGG	-	chr7.2:8447683-8447702	MS.gene050335:CDS	50.0%
!	TAACGTTTTGTGGGTCCCAC+AGG	-	chr7.2:8447761-8447780	MS.gene050335:CDS	50.0%
!!	GGCCTTCTGGAGTTTCACAT+CGG	+	chr7.2:8447393-8447412	None:intergenic	50.0%
	AACGCCTGTGGTTGAGGTTG+AGG	+	chr7.2:8447622-8447641	None:intergenic	55.0%
	ACGCCTGTGGTTGAGGTTGA+GGG	+	chr7.2:8447621-8447640	None:intergenic	55.0%
	GAAAGAGGACCGGGTTAACC+GGG	-	chr7.2:8447719-8447738	MS.gene050335:CDS	55.0%
	GATGCTTCAGGCACGATCGA+CGG	+	chr7.2:8447652-8447671	None:intergenic	55.0%
	GGACGTGTAGAAAGAGGACC+GGG	-	chr7.2:8447710-8447729	MS.gene050335:CDS	55.0%
	GGGACGTGTAGAAAGAGGAC+CGG	-	chr7.2:8447709-8447728	MS.gene050335:CDS	55.0%
	GTCGATCGTGCCTGAAGCAT+CGG	-	chr7.2:8447651-8447670	MS.gene050335:CDS	55.0%
	TCTCCCTCAACCTCAACCAC+AGG	-	chr7.2:8447615-8447634	MS.gene050335:CDS	55.0%
	TCTTTCTACACGTCCCGCAC+CGG	+	chr7.2:8447705-8447724	None:intergenic	55.0%
	TTCAGGCACGATCGACGGTA+CGG	+	chr7.2:8447647-8447666	None:intergenic	55.0%
	TTGTGGGTCCCACAGGTATC+CGG	-	chr7.2:8447768-8447787	MS.gene050335:CDS	55.0%
	AACGAACACGGCGGTGGAAG+CGG	+	chr7.2:8447566-8447585	None:intergenic	60.0%
	ACGGTCAACGCCTGTGGTTG+AGG	+	chr7.2:8447628-8447647	None:intergenic	60.0%
	CCGGTTCAATCGAACCGGTG+CGG	-	chr7.2:8447688-8447707	MS.gene050335:CDS	60.0%
	CGAAGCAGCAACGAACACGG+CGG	+	chr7.2:8447575-8447594	None:intergenic	60.0%
	CGGTTCAATCGAACCGGTGC+GGG	-	chr7.2:8447689-8447708	MS.gene050335:CDS	60.0%
	GGGCGAAGCAGCAACGAACA+CGG	+	chr7.2:8447578-8447597	None:intergenic	60.0%
	GGTGCGGGACGTGTAGAAAG+AGG	-	chr7.2:8447704-8447723	MS.gene050335:CDS	60.0%
!!	CCGCACCGGTTCGATTGAAC+CGG	+	chr7.2:8447691-8447710	None:intergenic	60.0%
	AGCAGCAACGAACACGGCGG+TGG	+	chr7.2:8447572-8447591	None:intergenic	65.0%
	GACGGTACGGTCAACGCCTG+TGG	+	chr7.2:8447634-8447653	None:intergenic	65.0%
	GCGCAGAGTCTGTGGCCTTC+TGG	+	chr7.2:8447406-8447425	None:intergenic	65.0%
	GGTGGAAGCGGATGCTGAGG+CGG	+	chr7.2:8447554-8447573	None:intergenic	65.0%
!	CGTTGCTGCTTCGCCCGTTG+CGG	-	chr7.2:8447582-8447601	MS.gene050335:CDS	65.0%
	GGAAGCGGATGCTGAGGCGG+AGG	+	chr7.2:8447551-8447570	None:intergenic	70.0%
	GGCGGTGGAAGCGGATGCTG+AGG	+	chr7.2:8447557-8447576	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	8447376	8447888	8447376	ID=MS.gene050335
chr7.2	mRNA	8447376	8447888	8447376	ID=MS.gene050335.t1;Parent=MS.gene050335
chr7.2	exon	8447376	8447888	8447376	ID=MS.gene050335.t1.exon1;Parent=MS.gene050335.t1
chr7.2	CDS	8447376	8447888	8447376	ID=cds.MS.gene050335.t1;Parent=MS.gene050335.t1

Gene Sequence

>MS.gene050335

ATGGCGGAAGAGCACCGATGTGAAACTCCAGAAGGCCACAGACTCTGCGCAAACAACTGCGGCTTCTTCGGCAGTTCAGCTACGATGAATCTCTGTTCAAAATGTTACAGAGACATCCATTTGAAGGAACAAGAACAAGCTAAAACTAAATCAACAATCGAAACGGCTCTCTCCTCCGCCTCAGCATCCGCTTCCACCGCCGTGTTCGTTGCTGCTTCGCCCGTTGCGGAAATCGAATCTCTCCCTCAACCTCAACCACAGGCGTTGACCGTACCGTCGATCGTGCCTGAAGCATCGGACAATTCACAAGGACCGGTTCAATCGAACCGGTGCGGGACGTGTAGAAAGAGGACCGGGTTAACCGGGTTCAAGTGTAGATGTGGAATAACGTTTTGTGGGTCCCACAGGTATCCGGAGAAACATGAGTGCGGTTTTGATTTCAAAGCAGTGGGGAGAGAGGAGATAGCGAAAGCGAATCCGGTGATCAAAGCTGATAAGCTCAGAAGAATTTGA

Protein sequence

>MS.gene050335.t1

MAEEHRCETPEGHRLCANNCGFFGSSATMNLCSKCYRDIHLKEQEQAKTKSTIETALSSASASASTAVFVAASPVAEIESLPQPQPQALTVPSIVPEASDNSQGPVQSNRCGTCRKRTGLTGFKCRCGITFCGSHRYPEKHECGFDFKAVGREEIAKANPVIKADKLRRI