AlfalfaGEDB | MS.gene038971

M. sativa cultivar XinJiangDaYe / MS.gene038971

KEGG: K02935
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00542.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038971.t1	GAU35832.1	89.4	216	23	0	1	216	1	216	7.40E-98	366.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038971.t1	P53163	40.4	146	80	3	78	216	49	194	4.2e-17	89.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038971.t1	A0A2K3P7T6	89.4	216	23	0	1	216	1	216	5.4e-98	366.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene60517	MS.gene038971	PPI
MS.gene68085	MS.gene038971	PPI
MS.gene005792	MS.gene038971	PPI
MS.gene038971	MS.gene09988	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038971.t1	MTR_2g064300	95.833	216	9	0	1	216	1	216	3.83e-143	398
MS.gene038971.t1	MTR_7g053520	83.871	217	34	1	1	216	1	217	3.36e-120	340
MS.gene038971.t1	MTR_2g031760	40.606	165	93	3	53	216	30	190	4.68e-33	118
MS.gene038971.t1	MTR_2g031960	47.857	140	66	3	79	216	21	155	7.82e-31	111
MS.gene038971.t1	MTR_8g076780	44.737	152	74	2	74	216	45	195	1.13e-29	109

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene038971.t1	AT1G70190	53.182	220	87	3	1	216	1	208	1.20e-65	201
MS.gene038971.t1	AT1G70190	53.182	220	87	3	1	216	1	208	1.20e-65	201
MS.gene038971.t1	AT4G36420	44.211	190	91	4	30	216	2	179	1.92e-34	121
MS.gene038971.t1	AT3G06040	41.611	149	80	1	68	216	45	186	5.70e-28	104
MS.gene038971.t1	AT3G06040	41.611	149	80	1	68	216	45	186	5.70e-28	104
MS.gene038971.t1	AT3G06040	41.611	149	80	1	68	216	45	186	5.70e-28	104
MS.gene038971.t1	AT4G37660	38.798	183	89	3	34	216	8	167	4.53e-25	96.7
MS.gene038971.t1	AT2G03130	57.500	80	34	0	137	216	12	91	1.12e-15	70.5

Find 53 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTGATGACTTTAATTT+TGG	0.115213	2.4:-28729812	None:intergenic
CCAGTTTATGAAAGCGTATT+AGG	0.115413	2.4:+28729388	MS.gene038971:CDS
TGGAGGGCCACGATTGTTAT+TGG	0.240332	2.4:-28729548	None:intergenic
GAAAGCAGGGTCTGCAAATT+TGG	0.254350	2.4:+28729693	MS.gene038971:CDS
GTGCGGGGCTTTACCGATTT+AGG	0.299345	2.4:+28729835	MS.gene038971:CDS
CCTAATACGCTTTCATAAAC+TGG	0.317127	2.4:-28729388	None:intergenic
TGAGCTTGCTGTTGAGAATA+AGG	0.322760	2.4:+28729335	MS.gene038971:CDS
GCTAGCTGAAGGTGCGGAAT+TGG	0.332644	2.4:+28729615	MS.gene038971:CDS
CAGTTTATGAAAGCGTATTA+GGG	0.345172	2.4:+28729389	MS.gene038971:CDS
GCTTTCATATATCCCACATT+AGG	0.373781	2.4:-28729676	None:intergenic
TGCCTCCTTCAAACCTAAAT+CGG	0.380631	2.4:-28729848	None:intergenic
GGAGGGCCACGATTGTTATT+GGG	0.390326	2.4:-28729547	None:intergenic
GGAGGAAGACGTAGAGGAAG+TGG	0.397120	2.4:+28729474	MS.gene038971:CDS
ACTTGGTGCTAATGTTGTTT+TGG	0.411925	2.4:+28729957	MS.gene038971:CDS
CTAGCTGAAGGTGCGGAATT+GGG	0.422415	2.4:+28729616	MS.gene038971:CDS
TTATTGGGATCAAATGTTGC+AGG	0.430449	2.4:-28729532	None:intergenic
CTTTACCGATTTAGGTTTGA+AGG	0.453564	2.4:+28729843	MS.gene038971:CDS
TAAAGTCATCAAGGAGGTGC+GGG	0.464501	2.4:+28729819	MS.gene038971:CDS
GTCTGCAAATTTGGCCGGAA+TGG	0.467477	2.4:+28729702	MS.gene038971:CDS
CTCCTCTTGGGCTGCAGTCG+TGG	0.476435	2.4:-28729734	None:intergenic
AAAGGTGTTTCAAAGGAAGA+AGG	0.492660	2.4:+28729907	MS.gene038971:CDS
GTTGCAGGATCAAAATCAGC+TGG	0.495579	2.4:-28729517	None:intergenic
TATAAAGAAAGGTGTTTCAA+AGG	0.503102	2.4:+28729900	MS.gene038971:CDS
ACACCGTCTATTATAAAGAA+AGG	0.512263	2.4:+28729889	MS.gene038971:CDS
GATAAAAGAAATGCCTAATG+TGG	0.517109	2.4:+28729663	MS.gene038971:CDS
TGGTCAAGTTGCAAGAAAAG+AGG	0.520139	2.4:+28729450	MS.gene038971:CDS
AATGTGGGATATATGAAAGC+AGG	0.525305	2.4:+28729679	MS.gene038971:CDS
GCGTATTAGGGCTTAATGCT+AGG	0.525999	2.4:+28729401	MS.gene038971:CDS
ACACCTTTCTTTATAATAGA+CGG	0.526861	2.4:-28729892	None:intergenic
TTCCAAAATTAAAGTCATCA+AGG	0.526956	2.4:+28729810	MS.gene038971:CDS
AGCCTGAAAACTTTCTCTGA+TGG	0.532734	2.4:-28729568	None:intergenic
CTGAAAACTTTCTCTGATGG+AGG	0.535890	2.4:-28729565	None:intergenic
GCAGGGTCTGCAAATTTGGC+CGG	0.545113	2.4:+28729697	MS.gene038971:CDS
TTTCAGGCTTGTTGATGAAG+TGG	0.545631	2.4:+28729582	MS.gene038971:CDS
AGAGGAGGAGGAAGACGTAG+AGG	0.548567	2.4:+28729468	MS.gene038971:CDS
AGTTGCAAGAAAAGAGGAGG+AGG	0.555547	2.4:+28729456	MS.gene038971:CDS
TTTAGGTTTGAAGGAGGCAA+AGG	0.562989	2.4:+28729852	MS.gene038971:CDS
ATAAAAGAAATGCCTAATGT+GGG	0.594300	2.4:+28729664	MS.gene038971:CDS
TGAAAACTTTCTCTGATGGA+GGG	0.607842	2.4:-28729564	None:intergenic
GCGTCTCTAACGCTAGCTGA+AGG	0.607910	2.4:+28729604	MS.gene038971:CDS
TTAAAGTCATCAAGGAGGTG+CGG	0.612909	2.4:+28729818	MS.gene038971:CDS
ATGTGGGATATATGAAAGCA+GGG	0.613059	2.4:+28729680	MS.gene038971:CDS
ATAGAGAAAATGAAAGCACT+TGG	0.623385	2.4:+28729940	MS.gene038971:CDS
TACCGATTTAGGTTTGAAGG+AGG	0.626116	2.4:+28729846	MS.gene038971:CDS
CACGACTGCAGCCCAAGAGG+AGG	0.628136	2.4:+28729735	MS.gene038971:CDS
TTGCAGGATCAAAATCAGCT+GGG	0.640796	2.4:-28729516	None:intergenic
AGCCACGACTGCAGCCCAAG+AGG	0.642383	2.4:+28729732	MS.gene038971:CDS
ATAAGGCATCGTTTACACAA+TGG	0.647228	2.4:+28729352	MS.gene038971:CDS
TCAAGTTGCAAGAAAAGAGG+AGG	0.661992	2.4:+28729453	MS.gene038971:CDS
AAAGTCATCAAGGAGGTGCG+GGG	0.667732	2.4:+28729820	MS.gene038971:CDS
TTTGATCCCAATAACAATCG+TGG	0.681002	2.4:+28729541	MS.gene038971:CDS
TCTAACGCTAGCTGAAGGTG+CGG	0.697298	2.4:+28729609	MS.gene038971:CDS
CAAAATTAAAGTCATCAAGG+AGG	0.704497	2.4:+28729813	MS.gene038971:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTCAAATACAGTTTTTTC+TGG	-	chr2.4:28729766-28729785	None:intergenic	20.0%
!	ACACCTTTCTTTATAATAGA+CGG	-	chr2.4:28729895-28729914	None:intergenic	25.0%
!	ATAAAAGAAATGCCTAATGT+GGG	+	chr2.4:28729664-28729683	MS.gene038971:CDS	25.0%
!	TTCCAAAATTAAAGTCATCA+AGG	+	chr2.4:28729810-28729829	MS.gene038971:CDS	25.0%
!!!	AATGTATTTTCGAGAAACTT+TGG	+	chr2.4:28729430-28729449	MS.gene038971:CDS	25.0%
!!!	CTCATTTTGATGAAGAAAAT+GGG	+	chr2.4:28729640-28729659	MS.gene038971:CDS	25.0%
!!!	TATAAAGAAAGGTGTTTCAA+AGG	+	chr2.4:28729900-28729919	MS.gene038971:CDS	25.0%
!!!	TCATTTTGATGAAGAAAATG+GGG	+	chr2.4:28729641-28729660	MS.gene038971:CDS	25.0%
!!!	TCTCATTTTGATGAAGAAAA+TGG	+	chr2.4:28729639-28729658	MS.gene038971:CDS	25.0%
	ACACCGTCTATTATAAAGAA+AGG	+	chr2.4:28729889-28729908	MS.gene038971:CDS	30.0%
	CAAAATTAAAGTCATCAAGG+AGG	+	chr2.4:28729813-28729832	MS.gene038971:CDS	30.0%
	GATAAAAGAAATGCCTAATG+TGG	+	chr2.4:28729663-28729682	MS.gene038971:CDS	30.0%
!	CAGTTTATGAAAGCGTATTA+GGG	+	chr2.4:28729389-28729408	MS.gene038971:CDS	30.0%
!!	ATAGAGAAAATGAAAGCACT+TGG	+	chr2.4:28729940-28729959	MS.gene038971:CDS	30.0%
!!	CTCCTTGATGACTTTAATTT+TGG	-	chr2.4:28729815-28729834	None:intergenic	30.0%
	AATGTGGGATATATGAAAGC+AGG	+	chr2.4:28729679-28729698	MS.gene038971:CDS	35.0%
	ATGTGGGATATATGAAAGCA+GGG	+	chr2.4:28729680-28729699	MS.gene038971:CDS	35.0%
	CCTAATACGCTTTCATAAAC+TGG	-	chr2.4:28729391-28729410	None:intergenic	35.0%
	GCTTTCATATATCCCACATT+AGG	-	chr2.4:28729679-28729698	None:intergenic	35.0%
	TGAAAACTTTCTCTGATGGA+GGG	-	chr2.4:28729567-28729586	None:intergenic	35.0%
	TTATTGGGATCAAATGTTGC+AGG	-	chr2.4:28729535-28729554	None:intergenic	35.0%
	TTTGATCCCAATAACAATCG+TGG	+	chr2.4:28729541-28729560	MS.gene038971:CDS	35.0%
!	CCAGTTTATGAAAGCGTATT+AGG	+	chr2.4:28729388-28729407	MS.gene038971:CDS	35.0%
!!	AAAGGTGTTTCAAAGGAAGA+AGG	+	chr2.4:28729907-28729926	MS.gene038971:CDS	35.0%
!!	ATAAGGCATCGTTTACACAA+TGG	+	chr2.4:28729352-28729371	MS.gene038971:CDS	35.0%
!!	CTTTACCGATTTAGGTTTGA+AGG	+	chr2.4:28729843-28729862	MS.gene038971:CDS	35.0%
!!!	ACTTGGTGCTAATGTTGTTT+TGG	+	chr2.4:28729957-28729976	MS.gene038971:CDS	35.0%
	AGCCTGAAAACTTTCTCTGA+TGG	-	chr2.4:28729571-28729590	None:intergenic	40.0%
	CTGAAAACTTTCTCTGATGG+AGG	-	chr2.4:28729568-28729587	None:intergenic	40.0%
	TGCCTCCTTCAAACCTAAAT+CGG	-	chr2.4:28729851-28729870	None:intergenic	40.0%
	TTAAAGTCATCAAGGAGGTG+CGG	+	chr2.4:28729818-28729837	MS.gene038971:CDS	40.0%
	TTGCAGGATCAAAATCAGCT+GGG	-	chr2.4:28729519-28729538	None:intergenic	40.0%
!	CTCCATCAGAGAAAGTTTTC+AGG	+	chr2.4:28729566-28729585	MS.gene038971:CDS	40.0%
!	TCAAGTTGCAAGAAAAGAGG+AGG	+	chr2.4:28729453-28729472	MS.gene038971:CDS	40.0%
!	TGGTCAAGTTGCAAGAAAAG+AGG	+	chr2.4:28729450-28729469	MS.gene038971:CDS	40.0%
!	TTTAGGTTTGAAGGAGGCAA+AGG	+	chr2.4:28729852-28729871	MS.gene038971:CDS	40.0%
!!	TACCGATTTAGGTTTGAAGG+AGG	+	chr2.4:28729846-28729865	MS.gene038971:CDS	40.0%
!!	TGAGCTTGCTGTTGAGAATA+AGG	+	chr2.4:28729335-28729354	MS.gene038971:CDS	40.0%
!!	TTTCAGGCTTGTTGATGAAG+TGG	+	chr2.4:28729582-28729601	MS.gene038971:CDS	40.0%
!!!	TTCTGGTTTTACCTCCTCTT+GGG	-	chr2.4:28729749-28729768	None:intergenic	40.0%
!!!	TTTCTGGTTTTACCTCCTCT+TGG	-	chr2.4:28729750-28729769	None:intergenic	40.0%
	AGTTGCAAGAAAAGAGGAGG+AGG	+	chr2.4:28729456-28729475	MS.gene038971:CDS	45.0%
	GAAAGCAGGGTCTGCAAATT+TGG	+	chr2.4:28729693-28729712	MS.gene038971:CDS	45.0%
	GCGTATTAGGGCTTAATGCT+AGG	+	chr2.4:28729401-28729420	MS.gene038971:CDS	45.0%
	GTTGCAGGATCAAAATCAGC+TGG	-	chr2.4:28729520-28729539	None:intergenic	45.0%
!	TAAAGTCATCAAGGAGGTGC+GGG	+	chr2.4:28729819-28729838	MS.gene038971:CDS	45.0%
	GTCTGCAAATTTGGCCGGAA+TGG	+	chr2.4:28729702-28729721	MS.gene038971:CDS	50.0%
	TCTAACGCTAGCTGAAGGTG+CGG	+	chr2.4:28729609-28729628	MS.gene038971:CDS	50.0%
	TGGAGGGCCACGATTGTTAT+TGG	-	chr2.4:28729551-28729570	None:intergenic	50.0%
!	AAAGTCATCAAGGAGGTGCG+GGG	+	chr2.4:28729820-28729839	MS.gene038971:CDS	50.0%
!	CGTGGCTTTTGTTGCCATTC+CGG	-	chr2.4:28729719-28729738	None:intergenic	50.0%
!	CTAGCTGAAGGTGCGGAATT+GGG	+	chr2.4:28729616-28729635	MS.gene038971:CDS	50.0%
!	GGAGGGCCACGATTGTTATT+GGG	-	chr2.4:28729550-28729569	None:intergenic	50.0%
	AGAGGAGGAGGAAGACGTAG+AGG	+	chr2.4:28729468-28729487	MS.gene038971:CDS	55.0%
	GCAGGGTCTGCAAATTTGGC+CGG	+	chr2.4:28729697-28729716	MS.gene038971:CDS	55.0%
	GCGTCTCTAACGCTAGCTGA+AGG	+	chr2.4:28729604-28729623	MS.gene038971:CDS	55.0%
	GGAGGAAGACGTAGAGGAAG+TGG	+	chr2.4:28729474-28729493	MS.gene038971:CDS	55.0%
!	GCTAGCTGAAGGTGCGGAAT+TGG	+	chr2.4:28729615-28729634	MS.gene038971:CDS	55.0%
!!	GTGCGGGGCTTTACCGATTT+AGG	+	chr2.4:28729835-28729854	MS.gene038971:CDS	55.0%
	AGCCACGACTGCAGCCCAAG+AGG	+	chr2.4:28729732-28729751	MS.gene038971:CDS	65.0%
	CACGACTGCAGCCCAAGAGG+AGG	+	chr2.4:28729735-28729754	MS.gene038971:CDS	65.0%
	CTCCTCTTGGGCTGCAGTCG+TGG	-	chr2.4:28729737-28729756	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	28729334	28729984	28729334	ID=MS.gene038971
chr2.4	mRNA	28729334	28729984	28729334	ID=MS.gene038971.t1;Parent=MS.gene038971
chr2.4	exon	28729334	28729984	28729334	ID=MS.gene038971.t1.exon1;Parent=MS.gene038971.t1
chr2.4	CDS	28729334	28729984	28729334	ID=cds.MS.gene038971.t1;Parent=MS.gene038971.t1

Gene Sequence

>MS.gene038971

ATGAGCTTGCTGTTGAGAATAAGGCATCGTTTACACAATGGTTTGTATAGACAACCAGTTTATGAAAGCGTATTAGGGCTTAATGCTAGGAAATTAAATGTATTTTCGAGAAACTTTGGTCAAGTTGCAAGAAAAGAGGAGGAGGAAGACGTAGAGGAAGTGGAAATTGATCAAAGAAGTCTCCCAGCTGATTTTGATCCTGCAACATTTGATCCCAATAACAATCGTGGCCCTCCATCAGAGAAAGTTTTCAGGCTTGTTGATGAAGTGGCGTCTCTAACGCTAGCTGAAGGTGCGGAATTGGGTCTCATTTTGATGAAGAAAATGGGGATAAAAGAAATGCCTAATGTGGGATATATGAAAGCAGGGTCTGCAAATTTGGCCGGAATGGCAACAAAAGCCACGACTGCAGCCCAAGAGGAGGTAAAACCAGAAAAAACTGTATTTGAATTGAAACTGTTGTCGTATGAAGCAGCTTCCAAAATTAAAGTCATCAAGGAGGTGCGGGGCTTTACCGATTTAGGTTTGAAGGAGGCAAAGGATATTGTCGAAAAAACACCGTCTATTATAAAGAAAGGTGTTTCAAAGGAAGAAGGAGAACAGATAATAGAGAAAATGAAAGCACTTGGTGCTAATGTTGTTTTGGAGTGA

Protein sequence

>MS.gene038971.t1

MSLLLRIRHRLHNGLYRQPVYESVLGLNARKLNVFSRNFGQVARKEEEEDVEEVEIDQRSLPADFDPATFDPNNNRGPPSEKVFRLVDEVASLTLAEGAELGLILMKKMGIKEMPNVGYMKAGSANLAGMATKATTAAQEEVKPEKTVFELKLLSYEAASKIKVIKEVRGFTDLGLKEAKDIVEKTPSIIKKGVSKEEGEQIIEKMKALGANVVLE