Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040447.t1 | RHN44990.1 | 98.5 | 330 | 5 | 0 | 1 | 330 | 1 | 330 | 5.70E-190 | 673.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040447.t1 | P49730 | 85.5 | 330 | 47 | 1 | 1 | 330 | 1 | 329 | 3.5e-164 | 578.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040447.t1 | A0A396GVD1 | 98.5 | 330 | 5 | 0 | 1 | 330 | 1 | 330 | 4.1e-190 | 673.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene040447 | MS.gene034044 | PPI |
| MS.gene79484 | MS.gene040447 | PPI |
| MS.gene37310 | MS.gene040447 | PPI |
| MS.gene040447 | MS.gene034046 | PPI |
| MS.gene026793 | MS.gene040447 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040447.t1 | MTR_0268s0080 | 98.182 | 330 | 6 | 0 | 1 | 330 | 1 | 330 | 0.0 | 673 |
| MS.gene040447.t1 | MTR_1g050472 | 97.879 | 330 | 7 | 0 | 1 | 330 | 1 | 330 | 0.0 | 669 |
| MS.gene040447.t1 | MTR_7g029110 | 97.273 | 330 | 9 | 0 | 1 | 330 | 1 | 330 | 0.0 | 666 |
| MS.gene040447.t1 | MTR_8g469010 | 92.121 | 330 | 21 | 1 | 1 | 330 | 1 | 325 | 0.0 | 632 |
| MS.gene040447.t1 | MTR_4g117370 | 76.615 | 325 | 75 | 1 | 6 | 330 | 18 | 341 | 0.0 | 531 |
| MS.gene040447.t1 | MTR_6g005270 | 67.442 | 43 | 14 | 0 | 265 | 307 | 25 | 67 | 1.39e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040447.t1 | AT3G27060 | 83.434 | 332 | 53 | 2 | 1 | 330 | 1 | 332 | 0.0 | 556 |
| MS.gene040447.t1 | AT3G23580 | 76.923 | 325 | 74 | 1 | 6 | 330 | 18 | 341 | 0.0 | 521 |
Find 58 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGCATTTGCTTGTGTAGA+AGG | 0.323501 | 7.4:+72605691 | MS.gene040447:CDS |
| TGCAATCTGGAAACCGTAAA+AGG | 0.337118 | 7.4:-72605497 | None:intergenic |
| ATTTCTTTGAGAAGCGCGTT+GGG | 0.337568 | 7.4:+72606094 | MS.gene040447:CDS |
| TTTGTTGCTGATAGATTATT+GGG | 0.346573 | 7.4:+72605991 | MS.gene040447:CDS |
| AGTGGCGTTCACCGTCTGTT+AGG | 0.349203 | 7.4:-72605372 | None:intergenic |
| GTTTGTTGCTGATAGATTAT+TGG | 0.356307 | 7.4:+72605990 | MS.gene040447:CDS |
| AGGCTGATTGGGCGATGAAA+TGG | 0.358474 | 7.4:+72605635 | MS.gene040447:CDS |
| GGTGCGAGAAGTGGTTCTTC+TGG | 0.368731 | 7.4:-72605220 | None:intergenic |
| ACCGTAAAAGGCTCGTGCTT+CGG | 0.385674 | 7.4:-72605485 | None:intergenic |
| ATGTTCAGAATCCGTTTGAT+TGG | 0.390491 | 7.4:+72606040 | MS.gene040447:CDS |
| GCGCTTCCTTGCGCGTTGGT+TGG | 0.401864 | 7.4:+72605934 | MS.gene040447:CDS |
| GAAAATCGCGCAGAAGCTTC+CGG | 0.409737 | 7.4:-72605725 | None:intergenic |
| GGGAACATGCAGAATCGGTC+TGG | 0.418807 | 7.4:-72605247 | None:intergenic |
| ATGCAGAATCGGTCTGGGTT+TGG | 0.432958 | 7.4:-72605241 | None:intergenic |
| AGGCTCGTGCTTCGGAGACT+TGG | 0.439806 | 7.4:-72605477 | None:intergenic |
| TCAGAATCCGTTTGATTGGA+TGG | 0.443597 | 7.4:+72606044 | MS.gene040447:CDS |
| TCCGTCTGAGGCGGCGAAGA+AGG | 0.447235 | 7.4:-72605413 | None:intergenic |
| GCCTTCTTCGCCGCCTCAGA+CGG | 0.453049 | 7.4:+72605412 | MS.gene040447:CDS |
| CTGTTATGAATAGCTTGAAT+GGG | 0.453833 | 7.4:+72606133 | MS.gene040447:CDS |
| TACAATTTCCTTCACACGCT+CGG | 0.455423 | 7.4:-72605872 | None:intergenic |
| TCTGTTATGAATAGCTTGAA+TGG | 0.458759 | 7.4:+72606132 | MS.gene040447:CDS |
| CGACGCGCTTCCTTGCGCGT+TGG | 0.462960 | 7.4:+72605930 | MS.gene040447:CDS |
| CGAAATAAGCTCGTTTGAGA+AGG | 0.470666 | 7.4:-72605782 | None:intergenic |
| ATGGAATTGATTTCGCTTCA+AGG | 0.479094 | 7.4:+72606063 | MS.gene040447:CDS |
| ATCAATTCCATCCAATCAAA+CGG | 0.480364 | 7.4:-72606051 | None:intergenic |
| GTGGCGTTCACCGTCTGTTA+GGG | 0.484123 | 7.4:-72605371 | None:intergenic |
| GGAACATGCAGAATCGGTCT+GGG | 0.489835 | 7.4:-72605246 | None:intergenic |
| GCAATGCAGTTGAAATTGAA+AGG | 0.492366 | 7.4:+72605896 | MS.gene040447:CDS |
| GGAGGAAAGATCGACTTCTT+CGG | 0.499480 | 7.4:-72605329 | None:intergenic |
| GGCGGTCCAGAATGATGCTT+CGG | 0.505885 | 7.4:-72605308 | None:intergenic |
| AGTGGTTCTTCTGGAAAAGC+AGG | 0.510370 | 7.4:-72605211 | None:intergenic |
| GGGTGAACTTGGTTGTGAAA+AGG | 0.510958 | 7.4:+72606011 | MS.gene040447:CDS |
| GATAGATTATTGGGTGAACT+TGG | 0.513434 | 7.4:+72606000 | MS.gene040447:CDS |
| GGATTGGGAACATGCAGAAT+CGG | 0.521690 | 7.4:-72605252 | None:intergenic |
| AATTTCTTTGAGAAGCGCGT+TGG | 0.523856 | 7.4:+72606093 | MS.gene040447:CDS |
| TTCGAGGACAATTCCGTCTG+AGG | 0.536756 | 7.4:-72605425 | None:intergenic |
| TCTGGGTTTGGTGCGAGAAG+TGG | 0.546005 | 7.4:-72605229 | None:intergenic |
| CAATGCAGTTGAAATTGAAA+GGG | 0.547072 | 7.4:+72605897 | MS.gene040447:CDS |
| TGCGCGTTGGTTGGAATGAA+CGG | 0.550794 | 7.4:+72605943 | MS.gene040447:CDS |
| TGGAATTGATTTCGCTTCAA+GGG | 0.551257 | 7.4:+72606064 | MS.gene040447:CDS |
| CGAGGACGTGTGAGATGAAG+TGG | 0.551534 | 7.4:-72605390 | None:intergenic |
| GAGCTTATTTCGCGTGACGA+AGG | 0.555351 | 7.4:+72605793 | MS.gene040447:CDS |
| GGCGTGCCAGTGCTTGAGAT+CGG | 0.556207 | 7.4:-72605350 | None:intergenic |
| ATTGTCCTCGAAAATCTCGC+TGG | 0.568595 | 7.4:+72605436 | MS.gene040447:CDS |
| TTTCCTCCGATCTCAAGCAC+TGG | 0.569460 | 7.4:+72605344 | MS.gene040447:CDS |
| CACTGGCACGCCCTAACAGA+CGG | 0.581564 | 7.4:+72605361 | MS.gene040447:CDS |
| TCGTTTGAGAAGGTTAATCC+CGG | 0.587906 | 7.4:-72605772 | None:intergenic |
| TTCATTCCAACCAACGCGCA+AGG | 0.588514 | 7.4:-72605940 | None:intergenic |
| AATCGCTTATATATGTCTCA+AGG | 0.594122 | 7.4:-72605555 | None:intergenic |
| ACTAACTTCCGAGCGTGTGA+AGG | 0.594762 | 7.4:+72605864 | MS.gene040447:CDS |
| TTTCTTTGAGAAGCGCGTTG+GGG | 0.598671 | 7.4:+72606095 | MS.gene040447:CDS |
| GAGGACAATTCCGTCTGAGG+CGG | 0.603534 | 7.4:-72605422 | None:intergenic |
| ATGAATAGCTTGAATGGGAA+TGG | 0.603815 | 7.4:+72606138 | MS.gene040447:CDS |
| CTGAGGCGGCGAAGAAGGCG+AGG | 0.610767 | 7.4:-72605408 | None:intergenic |
| GTGCCAGTGCTTGAGATCGG+AGG | 0.637438 | 7.4:-72605347 | None:intergenic |
| GGAAAGATCGACTTCTTCGG+CGG | 0.687608 | 7.4:-72605326 | None:intergenic |
| GAAGAGAATACATCTCAGAG+TGG | 0.694344 | 7.4:-72605531 | None:intergenic |
| GCGTTGGTTGGAATGAACGG+TGG | 0.708152 | 7.4:+72605946 | MS.gene040447:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTGTTGCTGATAGATTATT+GGG | + | chr7.4:72605991-72606010 | MS.gene040447:CDS | 25.0% |
| AATCCAATACCCAAAAATCT+GGG | + | chr7.4:72605270-72605289 | MS.gene040447:CDS | 30.0% | |
| AATCGCTTATATATGTCTCA+AGG | - | chr7.4:72605558-72605577 | None:intergenic | 30.0% | |
| AATTCCATGTATCGCAAAAA+AGG | + | chr7.4:72605615-72605634 | MS.gene040447:CDS | 30.0% | |
| ATCAATTCCATCCAATCAAA+CGG | - | chr7.4:72606054-72606073 | None:intergenic | 30.0% | |
| CAATGCAGTTGAAATTGAAA+GGG | + | chr7.4:72605897-72605916 | MS.gene040447:CDS | 30.0% | |
| CTGTTATGAATAGCTTGAAT+GGG | + | chr7.4:72606133-72606152 | MS.gene040447:CDS | 30.0% | |
| TCTGTTATGAATAGCTTGAA+TGG | + | chr7.4:72606132-72606151 | MS.gene040447:CDS | 30.0% | |
| ! | GGATATTTTCAATTGCAATC+TGG | - | chr7.4:72605513-72605532 | None:intergenic | 30.0% |
| ! | GTTTGTTGCTGATAGATTAT+TGG | + | chr7.4:72605990-72606009 | MS.gene040447:CDS | 30.0% |
| ! | TGTACATTTCCCAGATTTTT+GGG | - | chr7.4:72605282-72605301 | None:intergenic | 30.0% |
| ! | TTGTACATTTCCCAGATTTT+TGG | - | chr7.4:72605283-72605302 | None:intergenic | 30.0% |
| !!! | AGATTTTTGGGTATTGGATT+GGG | - | chr7.4:72605270-72605289 | None:intergenic | 30.0% |
| !!! | ATTTTCTGGTTGAAAAAACG+AGG | + | chr7.4:72605742-72605761 | MS.gene040447:CDS | 30.0% |
| !!! | TGCTGATTTTTGCTTTAGTT+TGG | + | chr7.4:72606164-72606183 | MS.gene040447:CDS | 30.0% |
| AAAAAACGAGGTTTAATGCC+GGG | + | chr7.4:72605754-72605773 | MS.gene040447:CDS | 35.0% | |
| ATGAATAGCTTGAATGGGAA+TGG | + | chr7.4:72606138-72606157 | MS.gene040447:CDS | 35.0% | |
| ATGTTCAGAATCCGTTTGAT+TGG | + | chr7.4:72606040-72606059 | MS.gene040447:CDS | 35.0% | |
| CAATCCAATACCCAAAAATC+TGG | + | chr7.4:72605269-72605288 | MS.gene040447:CDS | 35.0% | |
| GAAAAAACGAGGTTTAATGC+CGG | + | chr7.4:72605753-72605772 | MS.gene040447:CDS | 35.0% | |
| GATAGATTATTGGGTGAACT+TGG | + | chr7.4:72606000-72606019 | MS.gene040447:CDS | 35.0% | |
| GCAATGCAGTTGAAATTGAA+AGG | + | chr7.4:72605896-72605915 | MS.gene040447:CDS | 35.0% | |
| TATCGCAAAAAAGGCTGATT+GGG | + | chr7.4:72605624-72605643 | MS.gene040447:CDS | 35.0% | |
| ! | ATGGAATTGATTTCGCTTCA+AGG | + | chr7.4:72606063-72606082 | MS.gene040447:CDS | 35.0% |
| ! | GTAGAAGGAATTTTCTTCTC+CGG | + | chr7.4:72605706-72605725 | MS.gene040447:CDS | 35.0% |
| ! | TGGAATTGATTTCGCTTCAA+GGG | + | chr7.4:72606064-72606083 | MS.gene040447:CDS | 35.0% |
| !!! | CAGATTTTTGGGTATTGGAT+TGG | - | chr7.4:72605271-72605290 | None:intergenic | 35.0% |
| !!! | TTTCCCAGATTTTTGGGTAT+TGG | - | chr7.4:72605276-72605295 | None:intergenic | 35.0% |
| CGAAATAAGCTCGTTTGAGA+AGG | - | chr7.4:72605785-72605804 | None:intergenic | 40.0% | |
| GAAGAGAATACATCTCAGAG+TGG | - | chr7.4:72605534-72605553 | None:intergenic | 40.0% | |
| GTATCGCAAAAAAGGCTGAT+TGG | + | chr7.4:72605623-72605642 | MS.gene040447:CDS | 40.0% | |
| GTTGCATTTGCTTGTGTAGA+AGG | + | chr7.4:72605691-72605710 | MS.gene040447:CDS | 40.0% | |
| TACAATTTCCTTCACACGCT+CGG | - | chr7.4:72605875-72605894 | None:intergenic | 40.0% | |
| TCAGAATCCGTTTGATTGGA+TGG | + | chr7.4:72606044-72606063 | MS.gene040447:CDS | 40.0% | |
| TCGTTTGAGAAGGTTAATCC+CGG | - | chr7.4:72605775-72605794 | None:intergenic | 40.0% | |
| TGCAATCTGGAAACCGTAAA+AGG | - | chr7.4:72605500-72605519 | None:intergenic | 40.0% | |
| ! | AAAAAGCCGAAGCATCATTC+TGG | + | chr7.4:72605302-72605321 | MS.gene040447:CDS | 40.0% |
| ! | AATTTCTTTGAGAAGCGCGT+TGG | + | chr7.4:72606093-72606112 | MS.gene040447:CDS | 40.0% |
| ! | ATTTCTTTGAGAAGCGCGTT+GGG | + | chr7.4:72606094-72606113 | MS.gene040447:CDS | 40.0% |
| !!! | TCAGCCTTTTTTGCGATACA+TGG | - | chr7.4:72605622-72605641 | None:intergenic | 40.0% |
| ATTGTCCTCGAAAATCTCGC+TGG | + | chr7.4:72605436-72605455 | MS.gene040447:CDS | 45.0% | |
| GGATTGGGAACATGCAGAAT+CGG | - | chr7.4:72605255-72605274 | None:intergenic | 45.0% | |
| GGGTGAACTTGGTTGTGAAA+AGG | + | chr7.4:72606011-72606030 | MS.gene040447:CDS | 45.0% | |
| ! | AGTGGTTCTTCTGGAAAAGC+AGG | - | chr7.4:72605214-72605233 | None:intergenic | 45.0% |
| ! | ATCTTCCAGCGAGATTTTCG+AGG | - | chr7.4:72605444-72605463 | None:intergenic | 45.0% |
| ! | GGAGGAAAGATCGACTTCTT+CGG | - | chr7.4:72605332-72605351 | None:intergenic | 45.0% |
| ! | TTTCTTTGAGAAGCGCGTTG+GGG | + | chr7.4:72606095-72606114 | MS.gene040447:CDS | 45.0% |
| ACCGTAAAAGGCTCGTGCTT+CGG | - | chr7.4:72605488-72605507 | None:intergenic | 50.0% | |
| ACTAACTTCCGAGCGTGTGA+AGG | + | chr7.4:72605864-72605883 | MS.gene040447:CDS | 50.0% | |
| AGGCTGATTGGGCGATGAAA+TGG | + | chr7.4:72605635-72605654 | MS.gene040447:CDS | 50.0% | |
| ATGCAGAATCGGTCTGGGTT+TGG | - | chr7.4:72605244-72605263 | None:intergenic | 50.0% | |
| GAAAATCGCGCAGAAGCTTC+CGG | - | chr7.4:72605728-72605747 | None:intergenic | 50.0% | |
| GAGCTTATTTCGCGTGACGA+AGG | + | chr7.4:72605793-72605812 | MS.gene040447:CDS | 50.0% | |
| GGAACATGCAGAATCGGTCT+GGG | - | chr7.4:72605249-72605268 | None:intergenic | 50.0% | |
| TTCATTCCAACCAACGCGCA+AGG | - | chr7.4:72605943-72605962 | None:intergenic | 50.0% | |
| TTCGAGGACAATTCCGTCTG+AGG | - | chr7.4:72605428-72605447 | None:intergenic | 50.0% | |
| TTTCCTCCGATCTCAAGCAC+TGG | + | chr7.4:72605344-72605363 | MS.gene040447:CDS | 50.0% | |
| ! | GAAGCTTCTGCGCGATTTTC+TGG | + | chr7.4:72605728-72605747 | MS.gene040447:CDS | 50.0% |
| ! | GGAAAGATCGACTTCTTCGG+CGG | - | chr7.4:72605329-72605348 | None:intergenic | 50.0% |
| ! | TCCGAAGCACGAGCCTTTTA+CGG | + | chr7.4:72605484-72605503 | MS.gene040447:CDS | 50.0% |
| !! | TGCGCGTTGGTTGGAATGAA+CGG | + | chr7.4:72605943-72605962 | MS.gene040447:CDS | 50.0% |
| AGTGGCGTTCACCGTCTGTT+AGG | - | chr7.4:72605375-72605394 | None:intergenic | 55.0% | |
| CGAGGACGTGTGAGATGAAG+TGG | - | chr7.4:72605393-72605412 | None:intergenic | 55.0% | |
| GAGGACAATTCCGTCTGAGG+CGG | - | chr7.4:72605425-72605444 | None:intergenic | 55.0% | |
| GGCGGTCCAGAATGATGCTT+CGG | - | chr7.4:72605311-72605330 | None:intergenic | 55.0% | |
| GGGAACATGCAGAATCGGTC+TGG | - | chr7.4:72605250-72605269 | None:intergenic | 55.0% | |
| GGTGCGAGAAGTGGTTCTTC+TGG | - | chr7.4:72605223-72605242 | None:intergenic | 55.0% | |
| GTGGCGTTCACCGTCTGTTA+GGG | - | chr7.4:72605374-72605393 | None:intergenic | 55.0% | |
| !! | GCGTTGGTTGGAATGAACGG+TGG | + | chr7.4:72605946-72605965 | MS.gene040447:CDS | 55.0% |
| !! | TCTGGGTTTGGTGCGAGAAG+TGG | - | chr7.4:72605232-72605251 | None:intergenic | 55.0% |
| AGGCTCGTGCTTCGGAGACT+TGG | - | chr7.4:72605480-72605499 | None:intergenic | 60.0% | |
| CACTGGCACGCCCTAACAGA+CGG | + | chr7.4:72605361-72605380 | MS.gene040447:CDS | 60.0% | |
| GGCGTGCCAGTGCTTGAGAT+CGG | - | chr7.4:72605353-72605372 | None:intergenic | 60.0% | |
| GTGCCAGTGCTTGAGATCGG+AGG | - | chr7.4:72605350-72605369 | None:intergenic | 60.0% | |
| GCCTTCTTCGCCGCCTCAGA+CGG | + | chr7.4:72605412-72605431 | MS.gene040447:CDS | 65.0% | |
| ! | GCGCTTCCTTGCGCGTTGGT+TGG | + | chr7.4:72605934-72605953 | MS.gene040447:CDS | 65.0% |
| ! | TCCGTCTGAGGCGGCGAAGA+AGG | - | chr7.4:72605416-72605435 | None:intergenic | 65.0% |
| CGACGCGCTTCCTTGCGCGT+TGG | + | chr7.4:72605930-72605949 | MS.gene040447:CDS | 70.0% | |
| CTGAGGCGGCGAAGAAGGCG+AGG | - | chr7.4:72605411-72605430 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 72605208 | 72606200 | 72605208 | ID=MS.gene040447 |
| chr7.4 | mRNA | 72605208 | 72606200 | 72605208 | ID=MS.gene040447.t1;Parent=MS.gene040447 |
| chr7.4 | exon | 72605208 | 72606200 | 72605208 | ID=MS.gene040447.t1.exon1;Parent=MS.gene040447.t1 |
| chr7.4 | CDS | 72605208 | 72606200 | 72605208 | ID=cds.MS.gene040447.t1;Parent=MS.gene040447.t1 |
| Gene Sequence |
| Protein sequence |
