AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene041141

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041141.t1 MTR_5g018270 96.840 538 17 0 1 538 1 538 0.0 1077
MS.gene041141.t1 MTR_8g479390 81.783 516 91 2 25 537 25 540 0.0 889
MS.gene041141.t1 MTR_8g479380 66.730 523 169 4 15 533 13 534 0.0 731
MS.gene041141.t1 MTR_4g132320 62.963 540 196 2 1 536 1 540 0.0 684
MS.gene041141.t1 MTR_4g049390 61.346 520 197 2 22 537 18 537 0.0 673
MS.gene041141.t1 MTR_4g048350 61.731 520 194 3 22 537 18 536 0.0 669
MS.gene041141.t1 MTR_4g075690 58.647 532 217 2 9 537 8 539 0.0 650
MS.gene041141.t1 MTR_3g115050 59.134 531 211 3 11 537 12 540 0.0 649
MS.gene041141.t1 MTR_2g088080 60.971 515 197 2 26 536 25 539 0.0 646
MS.gene041141.t1 MTR_1g026410 59.065 535 213 3 9 537 5 539 0.0 643
MS.gene041141.t1 MTR_4g083190 53.420 541 235 5 4 531 2 538 0.0 585
MS.gene041141.t1 MTR_3g083440 50.936 534 248 4 10 531 5 536 0.0 562
MS.gene041141.t1 MTR_2g088080 60.699 458 176 2 83 536 1 458 0.0 562
MS.gene041141.t1 MTR_3g083390 50.936 534 248 4 10 531 5 536 0.0 561
MS.gene041141.t1 MTR_4g083210 51.698 530 246 4 10 531 5 532 0.0 561
MS.gene041141.t1 MTR_3g083430 50.749 534 249 4 10 531 5 536 0.0 559
MS.gene041141.t1 MTR_3g083450 52.308 520 235 3 24 531 19 537 0.0 559
MS.gene041141.t1 MTR_3g083400 50.749 534 249 4 10 531 5 536 0.0 558
MS.gene041141.t1 MTR_4g075690 58.636 440 179 2 9 445 8 447 0.0 537
MS.gene041141.t1 MTR_4g101650 50.275 545 248 7 11 532 7 551 5.33e-180 519
MS.gene041141.t1 MTR_3g083410 50.407 492 231 3 52 531 10 500 6.48e-178 511
MS.gene041141.t1 MTR_3g008580 48.535 546 257 7 9 531 8 552 2.74e-176 510
MS.gene041141.t1 MTR_7g051440 50.667 525 237 8 28 531 26 549 1.46e-170 496
MS.gene041141.t1 MTR_1g090707 47.842 533 261 9 15 532 8 538 1.71e-158 464
MS.gene041141.t1 MTR_4g133150 56.585 205 68 3 138 338 24 211 1.99e-65 212
MS.gene041141.t1 MTR_3g078740 27.973 597 330 22 13 531 11 585 9.75e-56 197
MS.gene041141.t1 MTR_3g078700 27.178 574 315 19 13 531 11 536 2.48e-53 189
MS.gene041141.t1 MTR_4g015120 26.773 564 358 15 8 530 8 557 2.85e-53 189
MS.gene041141.t1 MTR_5g020620 30.467 535 310 20 1 490 4 521 3.30e-52 187
MS.gene041141.t1 MTR_5g020600 27.829 539 324 18 7 496 10 532 5.50e-52 186
MS.gene041141.t1 MTR_8g091390 28.120 569 335 17 21 535 24 572 1.30e-51 186
MS.gene041141.t1 MTR_5g069680 25.394 571 364 15 9 530 1 558 3.89e-49 178
MS.gene041141.t1 MTR_3g078730 27.521 585 313 22 7 531 2 535 2.19e-45 168
MS.gene041141.t1 MTR_5g073210 26.816 537 334 20 8 496 1 526 2.02e-44 165
MS.gene041141.t1 MTR_3g462760 24.675 539 359 14 31 530 26 556 3.05e-44 165
MS.gene041141.t1 MTR_5g081810 26.554 531 331 16 45 530 13 529 3.95e-44 164
MS.gene041141.t1 MTR_3g071890 26.769 523 329 14 10 486 12 526 2.38e-43 162
MS.gene041141.t1 MTR_3g101640 26.354 554 347 16 11 514 13 555 6.50e-42 158
MS.gene041141.t1 MTR_5g018290 94.030 67 4 0 472 538 5 71 7.90e-40 139
MS.gene041141.t1 MTR_3g101635 25.939 559 342 18 14 518 14 554 3.62e-39 150
MS.gene041141.t1 MTR_5g083360 26.617 541 325 20 7 490 2 527 4.11e-39 150
MS.gene041141.t1 MTR_7g065970 25.355 564 349 17 8 513 4 553 2.59e-38 148
MS.gene041141.t1 MTR_7g065980 25.627 558 342 18 33 530 27 571 3.43e-38 148
MS.gene041141.t1 MTR_7g062250 25.836 538 334 18 12 496 19 544 6.74e-38 147
MS.gene041141.t1 MTR_8g027375 24.226 549 368 16 9 514 12 555 5.04e-37 144
MS.gene041141.t1 MTR_2g008330 23.909 573 382 15 4 530 15 579 1.83e-35 140
MS.gene041141.t1 MTR_7g058690 24.630 540 347 18 7 496 17 546 3.44e-34 136
MS.gene041141.t1 MTR_7g060460 25.362 552 351 21 1 496 1 547 1.09e-33 135
MS.gene041141.t1 MTR_7g458880 24.723 542 354 19 7 496 7 546 1.20e-33 135
MS.gene041141.t1 MTR_7g062310 23.181 591 388 13 1 530 8 593 3.58e-33 134
MS.gene041141.t1 MTR_4g064530 23.933 539 354 15 3 490 12 545 1.74e-30 125
MS.gene041141.t1 MTR_4g019225 25.000 552 353 16 33 530 28 572 1.86e-30 125
MS.gene041141.t1 MTR_4g064530 23.836 537 353 15 3 488 12 543 5.81e-29 121
MS.gene041141.t1 MTR_4g133160 42.056 107 48 2 414 520 3 95 5.52e-20 85.1
MS.gene041141.t1 MTR_5g081790 31.081 148 81 5 360 488 275 420 1.47e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene041141.t1 AT5G66920 73.308 517 136 1 23 537 29 545 0.0 803
MS.gene041141.t1 AT4G37160 64.739 536 183 4 4 533 2 537 0.0 694
MS.gene041141.t1 AT2G23630 66.092 522 172 3 20 538 19 538 0.0 692
MS.gene041141.t1 AT2G23630 66.092 522 172 3 20 538 24 543 0.0 692
MS.gene041141.t1 AT4G22010 60.452 531 205 3 12 537 7 537 0.0 665
MS.gene041141.t1 AT1G76160 58.824 527 214 2 13 536 10 536 0.0 645
MS.gene041141.t1 AT1G21850 58.333 540 219 4 1 537 1 537 0.0 638
MS.gene041141.t1 AT1G21860 57.963 540 221 4 1 537 1 537 0.0 635
MS.gene041141.t1 AT1G41830 56.238 537 228 3 4 537 6 538 0.0 627
MS.gene041141.t1 AT4G38420 56.929 534 220 4 14 537 13 546 0.0 617
MS.gene041141.t1 AT1G55570 55.370 540 227 4 9 535 7 545 0.0 597
MS.gene041141.t1 AT3G13400 55.204 538 227 4 11 535 6 542 0.0 589
MS.gene041141.t1 AT3G13400 55.204 538 227 4 11 535 6 542 0.0 589
MS.gene041141.t1 AT1G55560 53.717 538 235 4 11 535 5 541 0.0 583
MS.gene041141.t1 AT3G13390 56.322 522 214 4 27 535 24 544 0.0 578
MS.gene041141.t1 AT4G37160 64.732 448 152 4 4 445 2 449 0.0 575
MS.gene041141.t1 AT4G28090 52.552 529 241 5 13 533 11 537 0.0 573
MS.gene041141.t1 AT4G12420 50.916 546 245 6 9 531 3 548 0.0 548
MS.gene041141.t1 AT4G12420 50.916 546 245 6 9 531 3 548 0.0 548
MS.gene041141.t1 AT5G48450 52.444 532 239 7 15 532 13 544 0.0 541
MS.gene041141.t1 AT5G48450 52.444 532 239 7 15 532 13 544 0.0 541
MS.gene041141.t1 AT4G38420 56.596 470 194 4 13 472 12 481 0.0 533
MS.gene041141.t1 AT1G75790 48.624 545 252 8 13 533 4 544 4.74e-179 515
MS.gene041141.t1 AT4G25240 49.728 551 254 9 3 531 2 551 8.83e-179 516
MS.gene041141.t1 AT4G25240 49.728 551 254 9 3 531 2 551 8.83e-179 516
MS.gene041141.t1 AT5G51480 49.811 530 241 10 26 531 23 551 1.37e-177 513
MS.gene041141.t1 AT4G38420 56.885 443 181 4 13 445 12 454 2.06e-176 505
MS.gene041141.t1 AT5G21105 28.522 575 333 19 15 531 22 576 1.80e-60 210
MS.gene041141.t1 AT4G39830 27.855 578 349 14 9 532 16 579 8.51e-58 202
MS.gene041141.t1 AT5G21100 28.468 555 320 18 34 531 27 561 1.02e-57 202
MS.gene041141.t1 AT5G21105 29.135 532 304 17 15 488 22 538 1.63e-57 201
MS.gene041141.t1 AT5G58910 26.655 574 353 19 11 530 8 567 7.90e-51 183
MS.gene041141.t1 AT5G58910 27.210 577 349 20 7 530 8 566 3.91e-50 181
MS.gene041141.t1 AT2G38080 24.522 575 372 16 1 530 1 558 8.24e-48 175
MS.gene041141.t1 AT5G58910 26.952 538 320 18 45 530 7 523 2.07e-45 167
MS.gene041141.t1 AT5G01190 25.830 542 348 13 33 530 27 558 7.25e-45 166
MS.gene041141.t1 AT2G30210 26.471 578 365 20 4 530 2 570 1.25e-44 166
MS.gene041141.t1 AT5G09360 27.140 549 321 20 7 500 14 538 1.57e-44 166
MS.gene041141.t1 AT5G03260 26.855 566 358 18 8 530 5 557 6.00e-44 164
MS.gene041141.t1 AT5G05390 27.221 529 328 17 9 490 7 525 3.64e-43 162
MS.gene041141.t1 AT4G39830 27.403 489 290 12 97 532 6 482 1.28e-42 159
MS.gene041141.t1 AT4G39830 27.403 489 290 12 97 532 8 484 1.64e-42 159
MS.gene041141.t1 AT5G01190 25.954 524 334 13 51 530 31 544 2.04e-42 159
MS.gene041141.t1 AT5G01050 24.742 582 368 16 3 532 7 570 2.75e-42 160
MS.gene041141.t1 AT2G46570 24.602 565 376 15 9 530 12 569 9.13e-39 149
MS.gene041141.t1 AT5G07130 26.439 556 343 21 14 514 9 553 4.40e-38 147
MS.gene041141.t1 AT5G48100 25.836 538 331 17 33 515 24 548 1.65e-37 146
MS.gene041141.t1 AT4G39830 26.176 489 283 12 97 532 8 471 1.81e-37 144
MS.gene041141.t1 AT5G01040 25.551 544 340 16 41 532 38 568 2.56e-36 143
MS.gene041141.t1 AT2G40370 25.594 547 329 19 10 490 6 540 6.31e-36 141
MS.gene041141.t1 AT5G21105 28.571 343 209 10 211 531 57 385 8.16e-35 135
MS.gene041141.t1 AT2G29130 25.245 511 336 15 31 496 30 539 1.24e-34 138
MS.gene041141.t1 AT1G18140 24.335 526 315 17 33 490 31 541 1.27e-33 135
MS.gene041141.t1 AT3G09220 24.015 533 346 15 11 496 13 533 1.07e-32 132
MS.gene041141.t1 AT5G60020 23.050 577 384 18 12 530 3 577 1.85e-30 125
MS.gene041141.t1 AT2G46570 25.103 486 316 14 88 530 4 484 6.69e-29 120
MS.gene041141.t1 AT5G60020 23.277 537 355 17 49 530 11 545 7.10e-29 120
MS.gene041141.t1 AT5G60020 23.978 538 349 18 12 491 3 538 7.54e-29 120
MS.gene041141.t1 AT5G07130 25.420 476 291 20 94 514 2 468 5.38e-24 105
MS.gene041141.t1 AT5G07130 25.420 476 291 20 94 514 2 468 5.38e-24 105
MS.gene041141.t1 AT3G09220 24.146 439 282 13 104 496 3 436 6.97e-22 99.0

Find 121 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAACCAAAAGAATATATT+TGG 0.166156 5.2:+10230704 MS.gene041141:CDS
TTGAGACAATCTTTAGATTT+AGG 0.168571 5.2:+10229715 MS.gene041141:CDS
CTCAATCAACTTTAGAATTC+AGG 0.185567 5.2:+10229953 MS.gene041141:CDS
GTCCTTTGGAGGTTGATTTA+AGG 0.210344 5.2:-10230078 None:intergenic
GCAAGTTTAAGAGGGGTATC+AGG 0.239219 5.2:-10230792 None:intergenic
CAAGTGAATCATATAGATTT+TGG 0.246728 5.2:-10230019 None:intergenic
CCACAACGCAGAGCATTATT+AGG 0.254641 5.2:-10231771 None:intergenic
TTTCAAACCAAGGATCAAAT+TGG 0.285144 5.2:+10229065 MS.gene041141:CDS
AATATTGCTGTCCAAGAGTT+TGG 0.289589 5.2:-10231621 None:intergenic
TTGATTAATGGTCAGTTTCC+TGG 0.297368 5.2:+10228380 MS.gene041141:CDS
TACACATGGACTGTGACTTA+TGG 0.307708 5.2:+10228224 MS.gene041141:CDS
TAATTTGAGTTTGGAGGAAT+AGG 0.313962 5.2:-10229032 None:intergenic
CCACAAGGGTCATTCCATTA+TGG 0.322201 5.2:+10230672 MS.gene041141:CDS
TTGGAATTATCTTTCCATAA+TGG 0.324968 5.2:-10230686 None:intergenic
AAACTCTTGGACAGCAATAT+TGG 0.326496 5.2:+10231623 MS.gene041141:CDS
CTCCATCAGGATTTGGATAA+GGG 0.326577 5.2:-10229178 None:intergenic
ATTGATTGCAGTTATGGTTC+TGG 0.329329 5.2:+10231106 MS.gene041141:intron
CAAGGCATCCACTAGATTAT+AGG 0.334627 5.2:-10231155 None:intergenic
CATAAAGCTGCTGGAGGGTT+TGG 0.337768 5.2:+10229119 MS.gene041141:CDS
GCTGATTACTTCAACATTCC+TGG 0.338827 5.2:+10230813 MS.gene041141:CDS
ATTGGCCAAATATATTCTTT+TGG 0.345775 5.2:-10230709 None:intergenic
TTCATGGCAAGATGGAGTTC+TGG 0.347094 5.2:+10228998 MS.gene041141:CDS
ATTAGGAATGTCGTATTCAT+TGG 0.349261 5.2:-10231754 None:intergenic
GTAAAATCTCCATCAGGATT+TGG 0.362098 5.2:-10229185 None:intergenic
TTGTAAGTATAATTTGAGTT+TGG 0.365073 5.2:-10229041 None:intergenic
CCTCACCAAGTTAGTAGAAA+TGG 0.367092 5.2:-10228467 None:intergenic
TGAAGCAAGCTAGAACTTAC+AGG 0.368666 5.2:+10230234 MS.gene041141:CDS
TGGAATGACCCTTGTGGGTT+AGG 0.375841 5.2:-10230666 None:intergenic
GACAGCAATATTGGTGTCAT+TGG 0.377118 5.2:+10231632 MS.gene041141:CDS
AAGATATTGTCTTTCCCATA+TGG 0.379656 5.2:-10231685 None:intergenic
ATTGATCAATGGTGCTGAAT+TGG 0.381881 5.2:-10230727 None:intergenic
ATGCAATCTTGGCACCTTGA+TGG 0.391608 5.2:+10230954 MS.gene041141:CDS
ATAAGTTCCAATTTGATCCT+TGG 0.398271 5.2:-10229072 None:intergenic
GTGAAAATAACATGCAATCT+TGG 0.409939 5.2:+10230943 MS.gene041141:CDS
TCAATCAACTTTAGAATTCA+GGG 0.421010 5.2:+10229954 MS.gene041141:CDS
CATAATGGAATGACCCTTGT+GGG 0.423157 5.2:-10230671 None:intergenic
TCTCCATCAGGATTTGGATA+AGG 0.425122 5.2:-10229179 None:intergenic
AACTCTCGTTCCTCAGCATC+AGG 0.430959 5.2:+10230170 MS.gene041141:CDS
ACTAAAATGCATAAAGCTGC+TGG 0.431983 5.2:+10229110 MS.gene041141:CDS
CCTGATGGGCTCCTTATCAA+TGG 0.433708 5.2:+10229754 MS.gene041141:CDS
TAAGTATAATTTGAGTTTGG+AGG 0.437329 5.2:-10229038 None:intergenic
CAAGTTCATTCCACCTTCAC+TGG 0.440625 5.2:+10229778 MS.gene041141:CDS
AGGGGTATCAGGGTTAATAT+AGG 0.445205 5.2:-10230781 None:intergenic
CTAAAACTAGTCGAAGTTGA+AGG 0.449028 5.2:+10229984 MS.gene041141:CDS
TTACTCTACTCATTGGGGAT+TGG 0.449944 5.2:+10229204 MS.gene041141:CDS
TCATGGCAAGATGGAGTTCT+GGG 0.450810 5.2:+10228999 MS.gene041141:CDS
TATAGGTATATCCAAACTCT+TGG 0.453504 5.2:+10231610 MS.gene041141:intron
CCATTGATAAGGAGCCCATC+AGG 0.455106 5.2:-10229754 None:intergenic
TATGGAACTCTCTCTCCTCT+TGG 0.456521 5.2:+10228242 MS.gene041141:CDS
AAGTAATCAGCAAGTTTAAG+AGG 0.459151 5.2:-10230801 None:intergenic
ATGAGCCATTTCTACTAACT+TGG 0.461538 5.2:+10228462 MS.gene041141:CDS
GCAACAATGTAATAGTCCTT+TGG 0.463420 5.2:-10230092 None:intergenic
ATATGTGCAGGACCATTAGA+TGG 0.466940 5.2:-10230861 None:intergenic
GGAACTTGAGATCAGCCATA+TGG 0.467224 5.2:+10231670 MS.gene041141:CDS
TCAGGATTTGGATAAGGGAT+TGG 0.469027 5.2:-10229173 None:intergenic
TTGGACAACAGTTGTATATG+AGG 0.472580 5.2:+10231706 MS.gene041141:CDS
AAAGCTGCTGGAGGGTTTGG+AGG 0.472975 5.2:+10229122 MS.gene041141:CDS
GACCAGTCATAATTGTCAGC+AGG 0.473259 5.2:-10230209 None:intergenic
CGGAGCTTTCCATTGATCAA+TGG 0.473382 5.2:-10230738 None:intergenic
ATTCAGACTACTCCATCTAA+TGG 0.483356 5.2:+10230849 MS.gene041141:CDS
CTGCGTTGTGGCAAAGCATT+GGG 0.484504 5.2:+10231783 MS.gene041141:CDS
TTCTCTTCATGATTTCATTG+AGG 0.488380 5.2:+10230905 MS.gene041141:CDS
TTAGATTTAGGAAAATCTCT+TGG 0.492903 5.2:+10229727 MS.gene041141:CDS
ATATGGGAAAGACAATATCT+TGG 0.497914 5.2:+10231687 MS.gene041141:CDS
TTCTACTAGCATCTGTCCAT+TGG 0.500133 5.2:-10231129 None:intergenic
GCAGTTATGGTTCTGGCCAA+TGG 0.502544 5.2:+10231113 MS.gene041141:intron
CTGAATTCTAAAGTTGATTG+AGG 0.506388 5.2:-10229952 None:intergenic
TATGTTACTTACCTTGTGGC+TGG 0.512638 5.2:-10229234 None:intergenic
GAACTTGAGATCAGCCATAT+GGG 0.512652 5.2:+10231671 MS.gene041141:CDS
AATGAACTTGACCATTGATA+AGG 0.514250 5.2:-10229765 None:intergenic
CCTAATAATGCTCTGCGTTG+TGG 0.524629 5.2:+10231771 MS.gene041141:CDS
ATCTCAAGTTCCACATACCT+TGG 0.525823 5.2:-10231659 None:intergenic
CAAGTTTAAGAGGGGTATCA+GGG 0.530536 5.2:-10230791 None:intergenic
AGTAGAGTAAAATCTCCATC+AGG 0.532598 5.2:-10229191 None:intergenic
AACAGTTGTATATGAGGGTG+TGG 0.532794 5.2:+10231712 MS.gene041141:CDS
ATAAACCCACATTTGAGATC+CGG 0.537702 5.2:-10229926 None:intergenic
ATCCCTTATCCAAATCCTGA+TGG 0.543230 5.2:+10229176 MS.gene041141:CDS
ATTACCTTGGTTACCAGTGA+AGG 0.544886 5.2:-10229791 None:intergenic
TTAGTAATCAAGTCAAGTCT+AGG 0.545141 5.2:-10228404 None:intergenic
AATTCAGCACCATTGATCAA+TGG 0.545343 5.2:+10230729 MS.gene041141:CDS
ACCAGTCATAATTGTCAGCA+GGG 0.547383 5.2:-10230208 None:intergenic
TCTGCGTTGTGGCAAAGCAT+TGG 0.552680 5.2:+10231782 MS.gene041141:CDS
CCCCTGCTGACAATTATGAC+TGG 0.556480 5.2:+10230207 MS.gene041141:CDS
AGTAATCAGCAAGTTTAAGA+GGG 0.556655 5.2:-10230800 None:intergenic
ACAATGTAATAGTCCTTTGG+AGG 0.562881 5.2:-10230089 None:intergenic
TAGAAGTACCTATAATCTAG+TGG 0.562923 5.2:+10231147 MS.gene041141:CDS
TGAATATCACTGAAGACTCC+AGG 0.564427 5.2:-10230831 None:intergenic
CATGTTCCGGATCTCAAATG+TGG 0.568521 5.2:+10229920 MS.gene041141:CDS
CCATAATGGAATGACCCTTG+TGG 0.569679 5.2:-10230672 None:intergenic
GATTCACTTGATGTACATGT+TGG 0.569946 5.2:+10230032 MS.gene041141:CDS
CATTGGATAACCAAGGTATG+TGG 0.570010 5.2:+10231649 MS.gene041141:CDS
TACCTTAAATCAACCTCCAA+AGG 0.573055 5.2:+10230076 MS.gene041141:CDS
ACCGATGTAGCTATATGTGC+AGG 0.576247 5.2:-10230873 None:intergenic
CTGTGCAGTGTGTCTAGTCA+AGG 0.577408 5.2:-10231173 None:intergenic
CTTGACTAGACACACTGCAC+AGG 0.578929 5.2:+10231174 MS.gene041141:CDS
AAGCTGCTGGAGGGTTTGGA+GGG 0.580367 5.2:+10229123 MS.gene041141:CDS
TATTGGTCACTCACTTGTTG+AGG 0.582846 5.2:-10228269 None:intergenic
TACTTACAAGTTTCAAACCA+AGG 0.587094 5.2:+10229055 MS.gene041141:CDS
ATCAAGTCAAGTCTAGGACC+AGG 0.590567 5.2:-10228398 None:intergenic
ATCTGACAGCAAATGCTGCT+AGG 0.593901 5.2:+10230643 MS.gene041141:CDS
ACCTTGGTTACCAGTGAAGG+TGG 0.610392 5.2:-10229788 None:intergenic
AAAATGCATAAAGCTGCTGG+AGG 0.614281 5.2:+10229113 MS.gene041141:CDS
AGGCAAGGGTCCTGATGCTG+AGG 0.616933 5.2:-10230180 None:intergenic
GCTGCTAGGCCTAACCCACA+AGG 0.622148 5.2:+10230657 MS.gene041141:CDS
AGTTTGAGTAAATCTTGTTG+AGG 0.622304 5.2:-10230114 None:intergenic
CCAGGAATGGCATTAAACAA+AGG 0.629619 5.2:+10228970 MS.gene041141:intron
ATGTTCCGGATCTCAAATGT+GGG 0.629630 5.2:+10229921 MS.gene041141:CDS
TGGACAACAGTTGTATATGA+GGG 0.631389 5.2:+10231707 MS.gene041141:CDS
TCCACCTTCACTGGTAACCA+AGG 0.636980 5.2:+10229787 MS.gene041141:CDS
AGACACTATTAACTGCATAA+CGG 0.638349 5.2:-10230758 None:intergenic
TCCTGCACATATAGCTACAT+CGG 0.643778 5.2:+10230872 MS.gene041141:CDS
AAATGCATAAAGCTGCTGGA+GGG 0.654387 5.2:+10229114 MS.gene041141:CDS
CAGTCATAATTGTCAGCAGG+GGG 0.660164 5.2:-10230206 None:intergenic
CTGCTAGGCCTAACCCACAA+GGG 0.669842 5.2:+10230658 MS.gene041141:CDS
GTAATCAGCAAGTTTAAGAG+GGG 0.686146 5.2:-10230799 None:intergenic
TTGGTATAAGACCAGCCACA+AGG 0.697545 5.2:+10229223 MS.gene041141:CDS
CCAGTCATAATTGTCAGCAG+GGG 0.699494 5.2:-10230207 None:intergenic
ATGATTATAAGTTCTACACA+TGG 0.702965 5.2:+10228210 MS.gene041141:CDS
ACTTGTTGAGGAGTACCAAG+AGG 0.729297 5.2:-10228257 None:intergenic
TTGGTGTCATTGGATAACCA+AGG 0.736898 5.2:+10231642 MS.gene041141:CDS
GTGGAATGCACAACGCACTG+CGG 0.747881 5.2:+10231731 MS.gene041141:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGAATGATATTTAATATA+GGG - chr5.2:10229286-10229305 None:intergenic 10.0%
!! TGTTACATATTATAAAAAAA+AGG + chr5.2:10229339-10229358 MS.gene041141:intron 10.0%
!!! ATTTTTATATGATTAGAATT+TGG + chr5.2:10231336-10231355 MS.gene041141:intron 10.0%
!! ATAAAAACCAAATAAACATT+GGG - chr5.2:10231560-10231579 None:intergenic 15.0%
!! GTATGAATGATATTTAATAT+AGG - chr5.2:10229287-10229306 None:intergenic 15.0%
!! TATAAAAACCAAATAAACAT+TGG - chr5.2:10231561-10231580 None:intergenic 15.0%
!!! ATATTTAAGTTTATTTCAAC+AGG + chr5.2:10231282-10231301 MS.gene041141:intron 15.0%
!!! CATATCAATGTTTTAAAAAT+CGG - chr5.2:10228844-10228863 None:intergenic 15.0%
!!! CATTTTAAAAAAATCATCTA+TGG + chr5.2:10229438-10229457 MS.gene041141:intron 15.0%
!!! TTAAAATGTGATTTTGATTA+TGG - chr5.2:10229426-10229445 None:intergenic 15.0%
!! TTAAACAAAGGAAAAATTCA+TGG + chr5.2:10228982-10229001 MS.gene041141:CDS 20.0%
!! TTACATTTATTATGTCTTGT+AGG + chr5.2:10229690-10229709 MS.gene041141:intron 20.0%
!!! AAATCTAATCTCATATTTTG+GGG - chr5.2:10229648-10229667 None:intergenic 20.0%
!!! ATTTGCTTTGATTTGTTTAT+AGG + chr5.2:10230582-10230601 MS.gene041141:intron 20.0%
!!! CAAATCTAATCTCATATTTT+GGG - chr5.2:10229649-10229668 None:intergenic 20.0%
!!! TATCTGTTAAAATTTTAGCA+TGG - chr5.2:10229830-10229849 None:intergenic 20.0%
!!! TGTATTTAGTTTTCAAGTTA+TGG + chr5.2:10230278-10230297 MS.gene041141:intron 20.0%
!!! TTGAAGGTTATTTTGATTAA+TGG + chr5.2:10228368-10228387 MS.gene041141:intron 20.0%
!!! TTGTAAGTATAATTTGAGTT+TGG - chr5.2:10229044-10229063 None:intergenic 20.0%
!!! TTTAAAACACAAAATTACCT+TGG - chr5.2:10229807-10229826 None:intergenic 20.0%
!!! TTTAATATAGGGTGTTTATA+AGG - chr5.2:10229275-10229294 None:intergenic 20.0%
! AAAAACTGAACCAAATTAGT+CGG + chr5.2:10228581-10228600 MS.gene041141:intron 25.0%
! AAATCAAAGCAAATTCACAA+AGG - chr5.2:10230576-10230595 None:intergenic 25.0%
! AAATGAAATATTCTTGCAGA+TGG + chr5.2:10230619-10230638 MS.gene041141:intron 25.0%
! AACATTGATGTATACACAAA+GGG - chr5.2:10228559-10228578 None:intergenic 25.0%
! AATAGGTGGTTAAATATGTT+TGG + chr5.2:10230431-10230450 MS.gene041141:intron 25.0%
! AATGTTTAACACAGTGAAAT+AGG + chr5.2:10230414-10230433 MS.gene041141:intron 25.0%
! ACAATGTTAAATCATGTTCT+CGG - chr5.2:10228940-10228959 None:intergenic 25.0%
! AGAACATGATTTAACATTGT+TGG + chr5.2:10228940-10228959 MS.gene041141:intron 25.0%
! ATGATTATAAGTTCTACACA+TGG + chr5.2:10228210-10228229 MS.gene041141:CDS 25.0%
! GAACATGATTTAACATTGTT+GGG + chr5.2:10228941-10228960 MS.gene041141:intron 25.0%
! TAAATATCATTCATACTGCT+AGG + chr5.2:10229291-10229310 MS.gene041141:intron 25.0%
! TAAATGTAAGTTAACATGAC+AGG - chr5.2:10229679-10229698 None:intergenic 25.0%
! TATTTGTTTGCATTACTTGT+TGG + chr5.2:10228899-10228918 MS.gene041141:intron 25.0%
! TCACAGAAATATTATTCAGA+AGG - chr5.2:10231226-10231245 None:intergenic 25.0%
! TCCAACCAAAAGAATATATT+TGG + chr5.2:10230704-10230723 MS.gene041141:CDS 25.0%
! TGGCAATTATGTTCAATATA+TGG - chr5.2:10230485-10230504 None:intergenic 25.0%
! TTAGATTTAGGAAAATCTCT+TGG + chr5.2:10229727-10229746 MS.gene041141:CDS 25.0%
! TTGAGACAATCTTTAGATTT+AGG + chr5.2:10229715-10229734 MS.gene041141:CDS 25.0%
! TTGGAATTATCTTTCCATAA+TGG - chr5.2:10230689-10230708 None:intergenic 25.0%
!! ATTGGCCAAATATATTCTTT+TGG - chr5.2:10230712-10230731 None:intergenic 25.0%
!! CAAGTGAATCATATAGATTT+TGG - chr5.2:10230022-10230041 None:intergenic 25.0%
!! GATCCAATATAAAAGCATAT+TGG - chr5.2:10228289-10228308 None:intergenic 25.0%
!! GCAAATCTAATCTCATATTT+TGG - chr5.2:10229650-10229669 None:intergenic 25.0%
!! TAAGTATAATTTGAGTTTGG+AGG - chr5.2:10229041-10229060 None:intergenic 25.0%
!! TGACCAATATGCTTTTATAT+TGG + chr5.2:10228283-10228302 MS.gene041141:intron 25.0%
!! TTTTATATGCTGAATTGATG+TGG + chr5.2:10231574-10231593 MS.gene041141:intron 25.0%
!! TTTTTGTGGTTTCTTTTTGA+AGG + chr5.2:10228352-10228371 MS.gene041141:intron 25.0%
!!! AAACAAACCTTTAAAATTGG+CGG + chr5.2:10229622-10229641 MS.gene041141:intron 25.0%
!!! AGCAAACAAACCTTTAAAAT+TGG + chr5.2:10229619-10229638 MS.gene041141:intron 25.0%
!!! GCAATTTAGAATTTTCCTAA+TGG + chr5.2:10231058-10231077 MS.gene041141:intron 25.0%
!!! TCAATCAACTTTAGAATTCA+GGG + chr5.2:10229954-10229973 MS.gene041141:CDS 25.0%
AAAACAAAGTGCAAAACTGT+TGG - chr5.2:10229886-10229905 None:intergenic 30.0%
AAGATATTGTCTTTCCCATA+TGG - chr5.2:10231688-10231707 None:intergenic 30.0%
AAGTAATCAGCAAGTTTAAG+AGG - chr5.2:10230804-10230823 None:intergenic 30.0%
AAGTGCAAAAAGAAAAACAG+TGG - chr5.2:10228702-10228721 None:intergenic 30.0%
AATGAACTTGACCATTGATA+AGG - chr5.2:10229768-10229787 None:intergenic 30.0%
AGACACTATTAACTGCATAA+CGG - chr5.2:10230761-10230780 None:intergenic 30.0%
AGCATTATCAAAACACCATT+AGG - chr5.2:10231076-10231095 None:intergenic 30.0%
AGTAATCAGCAAGTTTAAGA+GGG - chr5.2:10230803-10230822 None:intergenic 30.0%
AGTTTGAGTAAATCTTGTTG+AGG - chr5.2:10230117-10230136 None:intergenic 30.0%
ATAAGTTCCAATTTGATCCT+TGG - chr5.2:10229075-10229094 None:intergenic 30.0%
ATATGGGAAAGACAATATCT+TGG + chr5.2:10231687-10231706 MS.gene041141:CDS 30.0%
ATTAGGAATGTCGTATTCAT+TGG - chr5.2:10231757-10231776 None:intergenic 30.0%
CAACATTGATGTATACACAA+AGG - chr5.2:10228560-10228579 None:intergenic 30.0%
CTGGTTAACACAATTCAAAT+AGG - chr5.2:10230508-10230527 None:intergenic 30.0%
GTGAAAATAACATGCAATCT+TGG + chr5.2:10230943-10230962 MS.gene041141:CDS 30.0%
GTGGTTACATATGAATGTAT+AGG + chr5.2:10231593-10231612 MS.gene041141:intron 30.0%
GTTAACACAATTCAAATAGG+TGG - chr5.2:10230505-10230524 None:intergenic 30.0%
TACTTACAAGTTTCAAACCA+AGG + chr5.2:10229055-10229074 MS.gene041141:CDS 30.0%
TATAGGTATATCCAAACTCT+TGG + chr5.2:10231610-10231629 MS.gene041141:intron 30.0%
TGGTTTAACTAGTTCAAAAG+TGG - chr5.2:10228783-10228802 None:intergenic 30.0%
TTAGTAATCAAGTCAAGTCT+AGG - chr5.2:10228407-10228426 None:intergenic 30.0%
TTATGTGCCCAATGTTTATT+TGG + chr5.2:10231550-10231569 MS.gene041141:intron 30.0%
TTCTCTTCATGATTTCATTG+AGG + chr5.2:10230905-10230924 MS.gene041141:CDS 30.0%
TTTCAAACCAAGGATCAAAT+TGG + chr5.2:10229065-10229084 MS.gene041141:CDS 30.0%
! AACATATGTTACTTACCTTG+TGG - chr5.2:10229241-10229260 None:intergenic 30.0%
! AGATTTTACTCTACTCATTG+GGG + chr5.2:10229199-10229218 MS.gene041141:CDS 30.0%
! ATATAGGGTGTTTATAAGGA+AGG - chr5.2:10229271-10229290 None:intergenic 30.0%
! GAGATTTTACTCTACTCATT+GGG + chr5.2:10229198-10229217 MS.gene041141:CDS 30.0%
! GCCAAATATATTCTTTTGGT+TGG - chr5.2:10230708-10230727 None:intergenic 30.0%
! TAATTTGAGTTTGGAGGAAT+AGG - chr5.2:10229035-10229054 None:intergenic 30.0%
! TATAGGGTGTTTATAAGGAA+GGG - chr5.2:10229270-10229289 None:intergenic 30.0%
!! ACCTTGATGGTTATGATTTT+TGG + chr5.2:10230967-10230986 MS.gene041141:CDS 30.0%
!! CCTTGATGGTTATGATTTTT+GGG + chr5.2:10230968-10230987 MS.gene041141:CDS 30.0%
!! CTCAATCAACTTTAGAATTC+AGG + chr5.2:10229953-10229972 MS.gene041141:CDS 30.0%
!! CTGAATTCTAAAGTTGATTG+AGG - chr5.2:10229955-10229974 None:intergenic 30.0%
!! TAGAAGTACCTATAATCTAG+TGG + chr5.2:10231147-10231166 MS.gene041141:CDS 30.0%
!!! AGTTTTGCACTTTGTTTTTC+AGG + chr5.2:10229888-10229907 MS.gene041141:intron 30.0%
!!! CAGATTCTGACTTTTTTTTG+TGG + chr5.2:10228338-10228357 MS.gene041141:intron 30.0%
!!! GCTTTATGCATTTTAGTTGA+TGG - chr5.2:10229107-10229126 None:intergenic 30.0%
!!! TGTTCTGTTGTTTTAGTTGA+TGG + chr5.2:10231028-10231047 MS.gene041141:intron 30.0%
AAACTCTTGGACAGCAATAT+TGG + chr5.2:10231623-10231642 MS.gene041141:CDS 35.0%
AACAAACCAACATGACAAAC+TGG - chr5.2:10228632-10228651 None:intergenic 35.0%
AACATTGTTGGGAATAAACC+AGG + chr5.2:10228952-10228971 MS.gene041141:intron 35.0%
AATATTGCTGTCCAAGAGTT+TGG - chr5.2:10231624-10231643 None:intergenic 35.0%
AATTCAGCACCATTGATCAA+TGG + chr5.2:10230729-10230748 MS.gene041141:CDS 35.0%
ACAATGTAATAGTCCTTTGG+AGG - chr5.2:10230092-10230111 None:intergenic 35.0%
ACTAAAATGCATAAAGCTGC+TGG + chr5.2:10229110-10229129 MS.gene041141:CDS 35.0%
ACTACTAATAAGACCTCTAG+AGG - chr5.2:10228809-10228828 None:intergenic 35.0%
AGGAAAAATTCATGGCAAGA+TGG + chr5.2:10228990-10229009 MS.gene041141:CDS 35.0%
AGTAGAGTAAAATCTCCATC+AGG - chr5.2:10229194-10229213 None:intergenic 35.0%
ATGAGCCATTTCTACTAACT+TGG + chr5.2:10228462-10228481 MS.gene041141:CDS 35.0%
ATTCAGACTACTCCATCTAA+TGG + chr5.2:10230849-10230868 MS.gene041141:CDS 35.0%
ATTGATTGCAGTTATGGTTC+TGG + chr5.2:10231106-10231125 MS.gene041141:intron 35.0%
CCACAGACACTTAAGATTAA+AGG + chr5.2:10231424-10231443 MS.gene041141:intron 35.0%
CCCAAAAATCATAACCATCA+AGG - chr5.2:10230971-10230990 None:intergenic 35.0%
CCTTTAATCTTAAGTGTCTG+TGG - chr5.2:10231427-10231446 None:intergenic 35.0%
CTAAAACTAGTCGAAGTTGA+AGG + chr5.2:10229984-10230003 MS.gene041141:CDS 35.0%
GATTCACTTGATGTACATGT+TGG + chr5.2:10230032-10230051 MS.gene041141:CDS 35.0%
GCAACAATGTAATAGTCCTT+TGG - chr5.2:10230095-10230114 None:intergenic 35.0%
GCTACAATTGATTGCAGTTA+TGG + chr5.2:10231100-10231119 MS.gene041141:intron 35.0%
GTAAAATCTCCATCAGGATT+TGG - chr5.2:10229188-10229207 None:intergenic 35.0%
GTAATCAGCAAGTTTAAGAG+GGG - chr5.2:10230802-10230821 None:intergenic 35.0%
GTTTAACACAGTGAAATAGG+TGG + chr5.2:10230417-10230436 MS.gene041141:intron 35.0%
TACCTTAAATCAACCTCCAA+AGG + chr5.2:10230076-10230095 MS.gene041141:CDS 35.0%
TCGCGAAAGTTGTCAAAATT+AGG + chr5.2:10230532-10230551 MS.gene041141:intron 35.0%
TGGACAACAGTTGTATATGA+GGG + chr5.2:10231707-10231726 MS.gene041141:CDS 35.0%
TGTACATGTCTGACACATTT+CGG + chr5.2:10231449-10231468 MS.gene041141:intron 35.0%
TTGATTAATGGTCAGTTTCC+TGG + chr5.2:10228380-10228399 MS.gene041141:CDS 35.0%
TTGGACAACAGTTGTATATG+AGG + chr5.2:10231706-10231725 MS.gene041141:CDS 35.0%
! ATAAACCCACATTTGAGATC+CGG - chr5.2:10229929-10229948 None:intergenic 35.0%
! ATTGATCAATGGTGCTGAAT+TGG - chr5.2:10230730-10230749 None:intergenic 35.0%
! GGAGATTTTACTCTACTCAT+TGG + chr5.2:10229197-10229216 MS.gene041141:CDS 35.0%
! TAAAGTTCTTCGTCGCATTT+TGG + chr5.2:10230324-10230343 MS.gene041141:intron 35.0%
!! AAATCTCTTGGTTTTCCTGA+TGG + chr5.2:10229739-10229758 MS.gene041141:CDS 35.0%
!! AATCTCTTGGTTTTCCTGAT+GGG + chr5.2:10229740-10229759 MS.gene041141:CDS 35.0%
!!! GTTATGATTTTTGGGTCGTT+GGG + chr5.2:10230976-10230995 MS.gene041141:CDS 35.0%
AAAATGCATAAAGCTGCTGG+AGG + chr5.2:10229113-10229132 MS.gene041141:CDS 40.0%
AAATGCATAAAGCTGCTGGA+GGG + chr5.2:10229114-10229133 MS.gene041141:CDS 40.0%
AACAGTTGTATATGAGGGTG+TGG + chr5.2:10231712-10231731 MS.gene041141:CDS 40.0%
ACCAGTCATAATTGTCAGCA+GGG - chr5.2:10230211-10230230 None:intergenic 40.0%
ACTGGTTGAACCGACTAATT+TGG - chr5.2:10228594-10228613 None:intergenic 40.0%
AGTTAAACCATGACCTCTAG+AGG + chr5.2:10228793-10228812 MS.gene041141:intron 40.0%
ATACACACCTGTTTACGAAC+AGG - chr5.2:10230353-10230372 None:intergenic 40.0%
ATATGTGCAGGACCATTAGA+TGG - chr5.2:10230864-10230883 None:intergenic 40.0%
ATCCCTTATCCAAATCCTGA+TGG + chr5.2:10229176-10229195 MS.gene041141:CDS 40.0%
ATCTCAAGTTCCACATACCT+TGG - chr5.2:10231662-10231681 None:intergenic 40.0%
ATGTTCCGGATCTCAAATGT+GGG + chr5.2:10229921-10229940 MS.gene041141:CDS 40.0%
ATTACCTTGGTTACCAGTGA+AGG - chr5.2:10229794-10229813 None:intergenic 40.0%
CAACCAGTTGAACTGGAAAT+CGG + chr5.2:10228606-10228625 MS.gene041141:intron 40.0%
CAGGAAAAACGTACATGTTC+CGG + chr5.2:10229907-10229926 MS.gene041141:intron 40.0%
CATTGGATAACCAAGGTATG+TGG + chr5.2:10231649-10231668 MS.gene041141:CDS 40.0%
CCAGGAATGGCATTAAACAA+AGG + chr5.2:10228970-10228989 MS.gene041141:intron 40.0%
CCTCACCAAGTTAGTAGAAA+TGG - chr5.2:10228470-10228489 None:intergenic 40.0%
CTCCATCAGGATTTGGATAA+GGG - chr5.2:10229181-10229200 None:intergenic 40.0%
GAACTTGAGATCAGCCATAT+GGG + chr5.2:10231671-10231690 MS.gene041141:CDS 40.0%
GCTGATTACTTCAACATTCC+TGG + chr5.2:10230813-10230832 MS.gene041141:CDS 40.0%
TCAGGATTTGGATAAGGGAT+TGG - chr5.2:10229176-10229195 None:intergenic 40.0%
TCCTGCACATATAGCTACAT+CGG + chr5.2:10230872-10230891 MS.gene041141:CDS 40.0%
TCTCCATCAGGATTTGGATA+AGG - chr5.2:10229182-10229201 None:intergenic 40.0%
TGAAGCAAGCTAGAACTTAC+AGG + chr5.2:10230234-10230253 MS.gene041141:CDS 40.0%
TGAATATCACTGAAGACTCC+AGG - chr5.2:10230834-10230853 None:intergenic 40.0%
TGTTGGGAATAAACCAGGAA+TGG + chr5.2:10228957-10228976 MS.gene041141:intron 40.0%
TTACTCTACTCATTGGGGAT+TGG + chr5.2:10229204-10229223 MS.gene041141:CDS 40.0%
TTCTACTAGCATCTGTCCAT+TGG - chr5.2:10231132-10231151 None:intergenic 40.0%
! AATAAGACCTCTAGAGGTCA+TGG - chr5.2:10228803-10228822 None:intergenic 40.0%
! CAAGGCATCCACTAGATTAT+AGG - chr5.2:10231158-10231177 None:intergenic 40.0%
! CATAATGGAATGACCCTTGT+GGG - chr5.2:10230674-10230693 None:intergenic 40.0%
! CCATTTCTACTAACTTGGTG+AGG + chr5.2:10228467-10228486 MS.gene041141:intron 40.0%
! CCTTTGTTTAATGCCATTCC+TGG - chr5.2:10228973-10228992 None:intergenic 40.0%
! GTCCTTTGGAGGTTGATTTA+AGG - chr5.2:10230081-10230100 None:intergenic 40.0%
! TACACATGGACTGTGACTTA+TGG + chr5.2:10228224-10228243 MS.gene041141:CDS 40.0%
! TATGTTACTTACCTTGTGGC+TGG - chr5.2:10229237-10229256 None:intergenic 40.0%
! TATTGGTCACTCACTTGTTG+AGG - chr5.2:10228272-10228291 None:intergenic 40.0%
!! AGGGGTATCAGGGTTAATAT+AGG - chr5.2:10230784-10230803 None:intergenic 40.0%
!! CAAGTTTAAGAGGGGTATCA+GGG - chr5.2:10230794-10230813 None:intergenic 40.0%
!! GACAGCAATATTGGTGTCAT+TGG + chr5.2:10231632-10231651 MS.gene041141:CDS 40.0%
!! TGCATTACTTGTTGGTTCGT+GGG + chr5.2:10228907-10228926 MS.gene041141:intron 40.0%
!! TTGCATTACTTGTTGGTTCG+TGG + chr5.2:10228906-10228925 MS.gene041141:intron 40.0%
!! TTGGTGTCATTGGATAACCA+AGG + chr5.2:10231642-10231661 MS.gene041141:CDS 40.0%
!!! GGTTATGATTTTTGGGTCGT+TGG + chr5.2:10230975-10230994 MS.gene041141:CDS 40.0%
AAAAACAGTGGAACCAGCCA+CGG - chr5.2:10228690-10228709 None:intergenic 45.0%
AATCGGCCAGTTTGTCATGT+TGG + chr5.2:10228623-10228642 MS.gene041141:intron 45.0%
ACCGATGTAGCTATATGTGC+AGG - chr5.2:10230876-10230895 None:intergenic 45.0%
ATCTGACAGCAAATGCTGCT+AGG + chr5.2:10230643-10230662 MS.gene041141:CDS 45.0%
ATGCAATCTTGGCACCTTGA+TGG + chr5.2:10230954-10230973 MS.gene041141:CDS 45.0%
CAACCAGACTGCGATACAAT+CGG - chr5.2:10228655-10228674 None:intergenic 45.0%
CAAGTTCATTCCACCTTCAC+TGG + chr5.2:10229778-10229797 MS.gene041141:CDS 45.0%
CAGTCATAATTGTCAGCAGG+GGG - chr5.2:10230209-10230228 None:intergenic 45.0%
CATGTTCCGGATCTCAAATG+TGG + chr5.2:10229920-10229939 MS.gene041141:CDS 45.0%
CCACAAGGGTCATTCCATTA+TGG + chr5.2:10230672-10230691 MS.gene041141:CDS 45.0%
CCAGTCATAATTGTCAGCAG+GGG - chr5.2:10230210-10230229 None:intergenic 45.0%
CCTAATAATGCTCTGCGTTG+TGG + chr5.2:10231771-10231790 MS.gene041141:CDS 45.0%
CGGAGCTTTCCATTGATCAA+TGG - chr5.2:10230741-10230760 None:intergenic 45.0%
GACCAGTCATAATTGTCAGC+AGG - chr5.2:10230212-10230231 None:intergenic 45.0%
GGAACTTGAGATCAGCCATA+TGG + chr5.2:10231670-10231689 MS.gene041141:CDS 45.0%
TATGGAACTCTCTCTCCTCT+TGG + chr5.2:10228242-10228261 MS.gene041141:CDS 45.0%
TCATGGCAAGATGGAGTTCT+GGG + chr5.2:10228999-10229018 MS.gene041141:CDS 45.0%
TGACAACTTTCGCGACTTTC+TGG - chr5.2:10230527-10230546 None:intergenic 45.0%
TTCATGGCAAGATGGAGTTC+TGG + chr5.2:10228998-10229017 MS.gene041141:CDS 45.0%
TTGGTATAAGACCAGCCACA+AGG + chr5.2:10229223-10229242 MS.gene041141:CDS 45.0%
TTGGTCTCCTGTTCGTAAAC+AGG + chr5.2:10230343-10230362 MS.gene041141:intron 45.0%
! ATCAAGTCAAGTCTAGGACC+AGG - chr5.2:10228401-10228420 None:intergenic 45.0%
! CCACAACGCAGAGCATTATT+AGG - chr5.2:10231774-10231793 None:intergenic 45.0%
! CCATAATGGAATGACCCTTG+TGG - chr5.2:10230675-10230694 None:intergenic 45.0%
!! ACTTGTTGAGGAGTACCAAG+AGG - chr5.2:10228260-10228279 None:intergenic 45.0%
!! GCAAGTTTAAGAGGGGTATC+AGG - chr5.2:10230795-10230814 None:intergenic 45.0%
!! TTTGGGGCCGCCAATTTTAA+AGG - chr5.2:10229632-10229651 None:intergenic 45.0%
AACTCTCGTTCCTCAGCATC+AGG + chr5.2:10230170-10230189 MS.gene041141:CDS 50.0%
ACCTTGGTTACCAGTGAAGG+TGG - chr5.2:10229791-10229810 None:intergenic 50.0%
ACGTGTCAGTGTTGTGTGCA+TGG + chr5.2:10231491-10231510 MS.gene041141:intron 50.0%
CATAAAGCTGCTGGAGGGTT+TGG + chr5.2:10229119-10229138 MS.gene041141:CDS 50.0%
CCATTGATAAGGAGCCCATC+AGG - chr5.2:10229757-10229776 None:intergenic 50.0%
CCCCTGCTGACAATTATGAC+TGG + chr5.2:10230207-10230226 MS.gene041141:CDS 50.0%
CTGTGCAGTGTGTCTAGTCA+AGG - chr5.2:10231176-10231195 None:intergenic 50.0%
CTTGACTAGACACACTGCAC+AGG + chr5.2:10231174-10231193 MS.gene041141:CDS 50.0%
GGTTGAACTGGATTGAACCG+TGG + chr5.2:10228670-10228689 MS.gene041141:intron 50.0%
GTATCGCAGTCTGGTTGAAC+TGG + chr5.2:10228658-10228677 MS.gene041141:intron 50.0%
GTTCCGATTGTATCGCAGTC+TGG + chr5.2:10228649-10228668 MS.gene041141:intron 50.0%
TCCACCTTCACTGGTAACCA+AGG + chr5.2:10229787-10229806 MS.gene041141:CDS 50.0%
TCTGCGTTGTGGCAAAGCAT+TGG + chr5.2:10231782-10231801 MS.gene041141:CDS 50.0%
TGGAATGACCCTTGTGGGTT+AGG - chr5.2:10230669-10230688 None:intergenic 50.0%
! CTGCGTTGTGGCAAAGCATT+GGG + chr5.2:10231783-10231802 MS.gene041141:CDS 50.0%
! GTCGGTTCAACCAGTTGAAC+TGG + chr5.2:10228599-10228618 MS.gene041141:intron 50.0%
! TGGCCGATTTCCAGTTCAAC+TGG - chr5.2:10228612-10228631 None:intergenic 50.0%
!! CCTGATGGGCTCCTTATCAA+TGG + chr5.2:10229754-10229773 MS.gene041141:CDS 50.0%
!! GCAGTTATGGTTCTGGCCAA+TGG + chr5.2:10231113-10231132 MS.gene041141:intron 50.0%
AAAGCTGCTGGAGGGTTTGG+AGG + chr5.2:10229122-10229141 MS.gene041141:CDS 55.0%
AACACTGACACGTCGACGCT+AGG - chr5.2:10231484-10231503 None:intergenic 55.0%
AAGCTGCTGGAGGGTTTGGA+GGG + chr5.2:10229123-10229142 MS.gene041141:CDS 55.0%
CTGCTAGGCCTAACCCACAA+GGG + chr5.2:10230658-10230677 MS.gene041141:CDS 55.0%
GAACTGGATTGAACCGTGGC+TGG + chr5.2:10228674-10228693 MS.gene041141:intron 55.0%
GTGGAATGCACAACGCACTG+CGG + chr5.2:10231731-10231750 MS.gene041141:CDS 55.0%
TAATTGTCAGCAGGGGGAGC+AGG - chr5.2:10230203-10230222 None:intergenic 55.0%
AGGCAAGGGTCCTGATGCTG+AGG - chr5.2:10230183-10230202 None:intergenic 60.0%
GACACGTCGACGCTAGGTGT+CGG - chr5.2:10231478-10231497 None:intergenic 60.0%
GCTGCTAGGCCTAACCCACA+AGG + chr5.2:10230657-10230676 MS.gene041141:CDS 60.0%
TCAGCAGGGGGAGCAGGCAA+GGG - chr5.2:10230197-10230216 None:intergenic 65.0%
GTCAGCAGGGGGAGCAGGCA+AGG - chr5.2:10230198-10230217 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 10228128 10231818 10228128 ID=MS.gene041141
chr5.2 mRNA 10228128 10231818 10228128 ID=MS.gene041141.t1;Parent=MS.gene041141
chr5.2 exon 10228128 10228277 10228128 ID=MS.gene041141.t1.exon1;Parent=MS.gene041141.t1
chr5.2 CDS 10228128 10228277 10228128 ID=cds.MS.gene041141.t1;Parent=MS.gene041141.t1
chr5.2 exon 10228374 10228483 10228374 ID=MS.gene041141.t1.exon2;Parent=MS.gene041141.t1
chr5.2 CDS 10228374 10228483 10228374 ID=cds.MS.gene041141.t1;Parent=MS.gene041141.t1
chr5.2 exon 10228974 10229244 10228974 ID=MS.gene041141.t1.exon3;Parent=MS.gene041141.t1
chr5.2 CDS 10228974 10229244 10228974 ID=cds.MS.gene041141.t1;Parent=MS.gene041141.t1
chr5.2 exon 10229712 10229808 10229712 ID=MS.gene041141.t1.exon4;Parent=MS.gene041141.t1
chr5.2 CDS 10229712 10229808 10229712 ID=cds.MS.gene041141.t1;Parent=MS.gene041141.t1
chr5.2 exon 10229910 10230255 10229910 ID=MS.gene041141.t1.exon5;Parent=MS.gene041141.t1
chr5.2 CDS 10229910 10230255 10229910 ID=cds.MS.gene041141.t1;Parent=MS.gene041141.t1
chr5.2 exon 10230638 10230997 10230638 ID=MS.gene041141.t1.exon6;Parent=MS.gene041141.t1
chr5.2 CDS 10230638 10230997 10230638 ID=cds.MS.gene041141.t1;Parent=MS.gene041141.t1
chr5.2 exon 10231117 10231195 10231117 ID=MS.gene041141.t1.exon7;Parent=MS.gene041141.t1
chr5.2 CDS 10231117 10231195 10231117 ID=cds.MS.gene041141.t1;Parent=MS.gene041141.t1
chr5.2 exon 10231615 10231818 10231615 ID=MS.gene041141.t1.exon8;Parent=MS.gene041141.t1
chr5.2 CDS 10231615 10231818 10231615 ID=cds.MS.gene041141.t1;Parent=MS.gene041141.t1
Gene Sequence

>MS.gene041141

ATGGGAAGCAAAAGCACTAGTTTGCTATCTCTACTATGTCTTGTTGTTGCATTAGTAAGTGTGTCGTTAGTGCAAGCAGAAGATGATTATAAGTTCTACACATGGACTGTGACTTATGGAACTCTCTCTCCTCTTGGTACTCCTCAACAAGTGAGTGACCAATATGCTTTTATATTGGATCTAAATCTAATAACTTTTTGTTAGAAAAAACAGATTCTGACTTTTTTTTGTGGTTTCTTTTTGAAGGTTATTTTGATTAATGGTCAGTTTCCTGGTCCTAGACTTGACTTGATTACTAATAACAATGTGATTCTCAATCTTATCAACAAGCTAGATGAGCCATTTCTACTAACTTGGTGAGGTTTTGTTTATGTTTTTATTTTCTTCTTGCTTGATTCAAGTTCTTTGCTTTATGATCTGCATTGAGTCCCTTTGTGTATACATCAATGTTGTAAAAACTGAACCAAATTAGTCGGTTCAACCAGTTGAACTGGAAATCGGCCAGTTTGTCATGTTGGTTTGTTCCGATTGTATCGCAGTCTGGTTGAACTGGATTGAACCGTGGCTGGTTCCACTGTTTTTCTTTTTGCACTTCAATTTACTTTTGTAACACTTTTTTACTGTTTATTACTTTATTTGTCATAAGCTTCAACCACTTTTGAACTAGTTAAACCATGACCTCTAGAGGTCTTATTAGTAGTTTGATCTTTCGTCCGATTTTTAAAACATTGATATGAAGTATCAACTTGTGTTTTCTTTTGTATGTTTTTCTATTTGTTTGCATTACTTGTTGGTTCGTGGGATTTTCTCCGAGAACATGATTTAACATTGTTGGGAATAAACCAGGAATGGCATTAAACAAAGGAAAAATTCATGGCAAGATGGAGTTCTGGGAACAAACTGTCCTATTCCTCCAAACTCAAATTATACTTACAAGTTTCAAACCAAGGATCAAATTGGAACTTATTCTTATTTTCCATCAACTAAAATGCATAAAGCTGCTGGAGGGTTTGGAGGGCTCAATGTTTATCGTAGATCTGTCATTCCAATCCCTTATCCAAATCCTGATGGAGATTTTACTCTACTCATTGGGGATTGGTATAAGACCAGCCACAAGGTAAGTAACATATGTTTTTTTTCCCTTCCTTATAAACACCCTATATTAAATATCATTCATACTGCTAGGCTGTTTGTGAGAATTAAGATCAATTTGTTACATATTATAAAAAAAAGGATTTTGACATTAAAAATTTATGTATTATTAAGAAAAGTAAAAGCTGATTTATGTTTGTTTACCATAATCAAAATCACATTTTAAAAAAATCATCTATGGAAAATCATTTTCAAGAACTACTCAAAAGAGCTTCTCATTTGAGACATTTTTAGATTTTGTTAAAATTCTAATTTTTTTTTTTAAAGTAATAACTAACAGATAAAAACTTCTGATATATTTTTTAAAAACAAGTAATTTTTTATAAAATTTTGTTTTGTAGCAAACAAACCTTTAAAATTGGCGGCCCCAAAATATGAGATTAGATTTGCTTGTTTCCTGTCATGTTAACTTACATTTATTATGTCTTGTAGGTATTGAGACAATCTTTAGATTTAGGAAAATCTCTTGGTTTTCCTGATGGGCTCCTTATCAATGGTCAAGTTCATTCCACCTTCACTGGTAACCAAGGTAATTTTGTGTTTTAAACCATGCTAAAATTTTAACAGATACTATACATTTTAACTTTGTAAAATTTGCTTTTACCAACAGTTTTGCACTTTGTTTTTCAGGAAAAACGTACATGTTCCGGATCTCAAATGTGGGTTTATCTACCTCAATCAACTTTAGAATTCAGGGTCATATGCTAAAACTAGTCGAAGTTGAAGGATCACATACTCTCCAAAATCTATATGATTCACTTGATGTACATGTTGGACAATCAGTTTCTGTGTTAGTTACCTTAAATCAACCTCCAAAGGACTATTACATTGTTGCCTCAACAAGATTTACTCAAACTGTTCTCAGCACAACTTCTGTGCTGCACTATTCAAACTCTCGTTCCTCAGCATCAGGACCCTTGCCTGCTCCCCCTGCTGACAATTATGACTGGTCTATGAAGCAAGCTAGAACTTACAGGTATTGAAGAAAGATTTAAATATGTATTTAGTTTTCAAGTTATGGTATTTTGCACTCTTTTTTCTCTCTAAAGTTCTTCGTCGCATTTTGGTCTCCTGTTCGTAAACAGGTGTGTATTTGAACAACTAAAATTTGACAACTTTTGCGACAACAATCTAAATGTTTAACACAGTGAAATAGGTGGTTAAATATGTTTGGTAGATGATTAATGTATATATCAACTTGACCATATATTGAACATAATTGCCACCTATTTGAATTGTGTTAACCAGAAAGTCGCGAAAGTTGTCAAAATTAGGTTCTTCGTAAAAGCAAAACCTTTGTGAATTTGCTTTGATTTGTTTATAGGTTTTAATCTGTTTGAAATGAAATATTCTTGCAGATGGAATCTGACAGCAAATGCTGCTAGGCCTAACCCACAAGGGTCATTCCATTATGGAAAGATAATTCCAACCAAAAGAATATATTTGGCCAATTCAGCACCATTGATCAATGGAAAGCTCCGTTATGCAGTTAATAGTGTCTCCTATATTAACCCTGATACCCCTCTTAAACTTGCTGATTACTTCAACATTCCTGGAGTCTTCAGTGATATTCAGACTACTCCATCTAATGGTCCTGCACATATAGCTACATCGGTGTTGCAAACTTCTCTTCATGATTTCATTGAGGTTGTGTTTCAAAACAGTGAAAATAACATGCAATCTTGGCACCTTGATGGTTATGATTTTTGGGTCGTTGGGTATGTTTGTTAGCTTCTTGATTTGAGTCTTGTTCTGTTGTTTTAGTTGATGGTGATGAAGCAATTTAGAATTTTCCTAATGGTGTTTTGATAATGCTCAATGCTACAATTGATTGCAGTTATGGTTCTGGCCAATGGACAGATGCTAGTAGAAGTACCTATAATCTAGTGGATGCCTTGACTAGACACACTGCACAGGTAATAAAACAAAACAAGTGTCGCATTCCTTCTGAATAATATTTCTGTGAGAAAATTTATGAAAACAACATATGATATGTCATAAGATATTTAAGTTTATTTCAACAGGTTTTCTAAAATAGTTTATGAAAACAGTTTGCATTTTTATATGATTAGAATTTGGCTGCATTATATTTAATTATAGAAATAACATATACACAAACACTTTGATAAGTTTTAAACTATGTACCACAGACACTTAAGATTAAAGGTGTGTACATGTCTGACACATTTCGGTGTCCGACACCTAGCGTCGACGTGTCAGTGTTGTGTGCATGGTCAATTCTGTGTATGTACTTCATAAGTCTTAATGCATTATGTGCCCAATGTTTATTTGGTTTTTATATGCTGAATTGATGTGGTTACATATGAATGTATAGGTATATCCAAACTCTTGGACAGCAATATTGGTGTCATTGGATAACCAAGGTATGTGGAACTTGAGATCAGCCATATGGGAAAGACAATATCTTGGACAACAGTTGTATATGAGGGTGTGGAATGCACAACGCACTGCGGCCAATGAATACGACATTCCTAATAATGCTCTGCGTTGTGGCAAAGCATTGGGACATCATCCTTAG
Protein sequence

>MS.gene041141.t1

MGSKSTSLLSLLCLVVALVSVSLVQAEDDYKFYTWTVTYGTLSPLGTPQQVILINGQFPGPRLDLITNNNVILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCPIPPNSNYTYKFQTKDQIGTYSYFPSTKMHKAAGGFGGLNVYRRSVIPIPYPNPDGDFTLLIGDWYKTSHKVLRQSLDLGKSLGFPDGLLINGQVHSTFTGNQGKTYMFRISNVGLSTSINFRIQGHMLKLVEVEGSHTLQNLYDSLDVHVGQSVSVLVTLNQPPKDYYIVASTRFTQTVLSTTSVLHYSNSRSSASGPLPAPPADNYDWSMKQARTYRWNLTANAARPNPQGSFHYGKIIPTKRIYLANSAPLINGKLRYAVNSVSYINPDTPLKLADYFNIPGVFSDIQTTPSNGPAHIATSVLQTSLHDFIEVVFQNSENNMQSWHLDGYDFWVVGYGSGQWTDASRSTYNLVDALTRHTAQVYPNSWTAILVSLDNQGMWNLRSAIWERQYLGQQLYMRVWNAQRTAANEYDIPNNALRCGKALGHHP