Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041717.t1 | RHN52506.1 | 98.1 | 209 | 4 | 0 | 1 | 209 | 162 | 370 | 1.50E-108 | 402.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041717.t1 | Q9SX73 | 32.0 | 175 | 110 | 2 | 3 | 177 | 111 | 276 | 7.2e-14 | 79.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041717.t1 | A0A396HGY0 | 98.1 | 209 | 4 | 0 | 1 | 209 | 162 | 370 | 1.1e-108 | 402.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene041717 | MS.gene42493 | PPI |
| MS.gene041717 | MS.gene92477 | PPI |
| MS.gene041717 | MS.gene40432 | PPI |
| MS.gene041717 | MS.gene05157 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041717.t1 | MTR_6g081040 | 92.823 | 209 | 15 | 0 | 1 | 209 | 119 | 327 | 1.54e-129 | 368 |
| MS.gene041717.t1 | MTR_6g081040 | 92.823 | 209 | 15 | 0 | 1 | 209 | 162 | 370 | 9.78e-129 | 367 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041717.t1 | AT3G17680 | 42.609 | 115 | 64 | 1 | 4 | 116 | 128 | 242 | 6.19e-21 | 89.0 |
| MS.gene041717.t1 | AT3G17680 | 42.609 | 115 | 64 | 1 | 4 | 116 | 160 | 274 | 8.84e-21 | 89.0 |
| MS.gene041717.t1 | AT3G17680 | 42.609 | 115 | 64 | 1 | 4 | 116 | 172 | 286 | 1.15e-20 | 88.6 |
| MS.gene041717.t1 | AT1G48405 | 36.719 | 128 | 76 | 1 | 3 | 130 | 111 | 233 | 1.42e-16 | 76.6 |
Find 36 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCCACTTAAACCTTCT+AGG | 0.254299 | 6.3:-70820882 | None:intergenic |
| TCTTCTCCAAACCATGTATC+TGG | 0.388286 | 6.3:-70820734 | None:intergenic |
| TCAAGGCCAGATACATGGTT+TGG | 0.410594 | 6.3:+70820728 | MS.gene041717:CDS |
| TTCAAATGGAGAAGCCTAGA+AGG | 0.411732 | 6.3:+70820868 | MS.gene041717:CDS |
| GAAGAGTTTGCTTGAGGTGT+TGG | 0.416910 | 6.3:+70820512 | MS.gene041717:CDS |
| TGGTGGTGAAGGGTTAGCTT+TGG | 0.430299 | 6.3:+70820836 | MS.gene041717:CDS |
| AGGAAGAAGGAAATTCTTCT+AGG | 0.441661 | 6.3:+70820901 | MS.gene041717:CDS |
| GAGTTATAGAGTTAATAGTT+TGG | 0.443249 | 6.3:+70820575 | MS.gene041717:CDS |
| GAAAGTATTGCGAGTCTTCG+AGG | 0.458677 | 6.3:+70820475 | MS.gene041717:CDS |
| TTTGGAAGTTCATGTTCAAA+TGG | 0.473753 | 6.3:+70820854 | MS.gene041717:CDS |
| ATCGGCAACAAAGGGAAGAA+TGG | 0.498887 | 6.3:+70820816 | MS.gene041717:CDS |
| GATCTTCAAGGCCAGATACA+TGG | 0.502154 | 6.3:+70820723 | MS.gene041717:CDS |
| AGAAGCCTAGAAGGTTTAAG+TGG | 0.505030 | 6.3:+70820877 | MS.gene041717:CDS |
| GGTTCATGAGGTTGGTGTCA+TGG | 0.507161 | 6.3:+70820449 | MS.gene041717:CDS |
| AGAAGAGAGAGTCAAGAAAA+CGG | 0.507190 | 6.3:+70820786 | MS.gene041717:CDS |
| AGGTTTAAGTGGAAGGAAGA+AGG | 0.509088 | 6.3:+70820888 | MS.gene041717:CDS |
| AGATACATGGTTTGGAGAAG+AGG | 0.525980 | 6.3:+70820736 | MS.gene041717:CDS |
| GCCTAGAAGGTTTAAGTGGA+AGG | 0.532394 | 6.3:+70820881 | MS.gene041717:CDS |
| AAAGGGAAGAATGGTGGTGA+AGG | 0.547748 | 6.3:+70820825 | MS.gene041717:CDS |
| AACATGGACTTGCTGCTATG+TGG | 0.560577 | 6.3:+70820972 | MS.gene041717:CDS |
| GATCAAGATCAAGCGCAAGT+TGG | 0.564528 | 6.3:-70821002 | None:intergenic |
| CAAAGAGCTTGCGTCCGAGT+CGG | 0.566207 | 6.3:+70820608 | MS.gene041717:CDS |
| AAGGGAAGAATGGTGGTGAA+GGG | 0.583396 | 6.3:+70820826 | MS.gene041717:CDS |
| ACATGGACTTGCTGCTATGT+GGG | 0.588821 | 6.3:+70820973 | MS.gene041717:CDS |
| AGGAAGATTGAAGATCTTCA+AGG | 0.589114 | 6.3:+70820711 | MS.gene041717:CDS |
| CTAAGTGTGTGCTGAAGATG+AGG | 0.598254 | 6.3:+70820661 | MS.gene041717:CDS |
| GTTACAGAAGAGTTTGCTTG+AGG | 0.610285 | 6.3:+70820506 | MS.gene041717:CDS |
| CACACACTTAGCTAACTCAC+CGG | 0.626155 | 6.3:-70820649 | None:intergenic |
| GTACTGTTGAATGAGAATGC+TGG | 0.626374 | 6.3:+70820549 | MS.gene041717:CDS |
| AATTGCATCAACATCAACCA+TGG | 0.628092 | 6.3:-70820385 | None:intergenic |
| TCTCTTTATGAAAACCGACT+CGG | 0.628900 | 6.3:-70820622 | None:intergenic |
| GGAACAAGGAGTATTATGAG+AGG | 0.665037 | 6.3:+70820757 | MS.gene041717:CDS |
| GGCAACAAAGGGAAGAATGG+TGG | 0.678048 | 6.3:+70820819 | MS.gene041717:CDS |
| TGGTTTGGAGAAGAGGAACA+AGG | 0.704148 | 6.3:+70820743 | MS.gene041717:CDS |
| GTGTGTGCTGAAGATGAGGG+AGG | 0.735654 | 6.3:+70820665 | MS.gene041717:CDS |
| TAAGTGTGTGCTGAAGATGA+GGG | 0.745974 | 6.3:+70820662 | MS.gene041717:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAGTTATAGAGTTAATAGTT+TGG | + | chr6.3:70820575-70820594 | MS.gene041717:CDS | 25.0% |
| !! | ATAGAGTTTTTCTATTGCAT+AGG | + | chr6.3:70820691-70820710 | MS.gene041717:CDS | 25.0% |
| !!! | GAATGTTATGTGTGATTTTT+TGG | + | chr6.3:70820428-70820447 | MS.gene041717:CDS | 25.0% |
| TTTGGAAGTTCATGTTCAAA+TGG | + | chr6.3:70820854-70820873 | MS.gene041717:CDS | 30.0% | |
| ! | AAGAATTTTGCTGGGAAAAA+AGG | + | chr6.3:70820924-70820943 | MS.gene041717:CDS | 30.0% |
| ! | GTTTTCATAAAGAGAAAAGC+CGG | + | chr6.3:70820630-70820649 | MS.gene041717:CDS | 30.0% |
| !!! | CAAGAAAACGGTAGTTTTAT+CGG | + | chr6.3:70820798-70820817 | MS.gene041717:CDS | 30.0% |
| AGAAGAGAGAGTCAAGAAAA+CGG | + | chr6.3:70820786-70820805 | MS.gene041717:CDS | 35.0% | |
| AGGAAGAAGGAAATTCTTCT+AGG | + | chr6.3:70820901-70820920 | MS.gene041717:CDS | 35.0% | |
| AGGAAGATTGAAGATCTTCA+AGG | + | chr6.3:70820711-70820730 | MS.gene041717:CDS | 35.0% | |
| GGAAAAAAGGTCATAGTTTG+TGG | + | chr6.3:70820937-70820956 | MS.gene041717:CDS | 35.0% | |
| GTGGAAAAAGTTGAAGAACA+TGG | + | chr6.3:70820956-70820975 | MS.gene041717:CDS | 35.0% | |
| TCTCTTTATGAAAACCGACT+CGG | - | chr6.3:70820625-70820644 | None:intergenic | 35.0% | |
| !! | CTTCTAGGAAGAATTTTGCT+GGG | + | chr6.3:70820916-70820935 | MS.gene041717:CDS | 35.0% |
| !! | TCTTCTAGGAAGAATTTTGC+TGG | + | chr6.3:70820915-70820934 | MS.gene041717:CDS | 35.0% |
| !!! | GATTTTTTGGTTCATGAGGT+TGG | + | chr6.3:70820441-70820460 | MS.gene041717:CDS | 35.0% |
| !!! | GTAGTTTTATCGGCAACAAA+GGG | + | chr6.3:70820808-70820827 | MS.gene041717:CDS | 35.0% |
| !!! | GTGTGATTTTTTGGTTCATG+AGG | + | chr6.3:70820437-70820456 | MS.gene041717:CDS | 35.0% |
| AGAAGCCTAGAAGGTTTAAG+TGG | + | chr6.3:70820877-70820896 | MS.gene041717:CDS | 40.0% | |
| AGGTTTAAGTGGAAGGAAGA+AGG | + | chr6.3:70820888-70820907 | MS.gene041717:CDS | 40.0% | |
| GTACTGTTGAATGAGAATGC+TGG | + | chr6.3:70820549-70820568 | MS.gene041717:CDS | 40.0% | |
| TAAGTGTGTGCTGAAGATGA+GGG | + | chr6.3:70820662-70820681 | MS.gene041717:CDS | 40.0% | |
| TCCTTCCACTTAAACCTTCT+AGG | - | chr6.3:70820885-70820904 | None:intergenic | 40.0% | |
| TCTTCTCCAAACCATGTATC+TGG | - | chr6.3:70820737-70820756 | None:intergenic | 40.0% | |
| TTCAAATGGAGAAGCCTAGA+AGG | + | chr6.3:70820868-70820887 | MS.gene041717:CDS | 40.0% | |
| ! | AGATACATGGTTTGGAGAAG+AGG | + | chr6.3:70820736-70820755 | MS.gene041717:CDS | 40.0% |
| ! | GTTACAGAAGAGTTTGCTTG+AGG | + | chr6.3:70820506-70820525 | MS.gene041717:CDS | 40.0% |
| !! | GGAACAAGGAGTATTATGAG+AGG | + | chr6.3:70820757-70820776 | MS.gene041717:CDS | 40.0% |
| !!! | GGTAGTTTTATCGGCAACAA+AGG | + | chr6.3:70820807-70820826 | MS.gene041717:CDS | 40.0% |
| ATCGGCAACAAAGGGAAGAA+TGG | + | chr6.3:70820816-70820835 | MS.gene041717:CDS | 45.0% | |
| CACACACTTAGCTAACTCAC+CGG | - | chr6.3:70820652-70820671 | None:intergenic | 45.0% | |
| CTAAGTGTGTGCTGAAGATG+AGG | + | chr6.3:70820661-70820680 | MS.gene041717:CDS | 45.0% | |
| GATCTTCAAGGCCAGATACA+TGG | + | chr6.3:70820723-70820742 | MS.gene041717:CDS | 45.0% | |
| GCCTAGAAGGTTTAAGTGGA+AGG | + | chr6.3:70820881-70820900 | MS.gene041717:CDS | 45.0% | |
| TCAAGGCCAGATACATGGTT+TGG | + | chr6.3:70820728-70820747 | MS.gene041717:CDS | 45.0% | |
| ! | AAAGGGAAGAATGGTGGTGA+AGG | + | chr6.3:70820825-70820844 | MS.gene041717:CDS | 45.0% |
| ! | AAGGGAAGAATGGTGGTGAA+GGG | + | chr6.3:70820826-70820845 | MS.gene041717:CDS | 45.0% |
| ! | GAAGAGTTTGCTTGAGGTGT+TGG | + | chr6.3:70820512-70820531 | MS.gene041717:CDS | 45.0% |
| ! | TGGTTTGGAGAAGAGGAACA+AGG | + | chr6.3:70820743-70820762 | MS.gene041717:CDS | 45.0% |
| !! | AACATGGACTTGCTGCTATG+TGG | + | chr6.3:70820972-70820991 | MS.gene041717:CDS | 45.0% |
| !! | ACATGGACTTGCTGCTATGT+GGG | + | chr6.3:70820973-70820992 | MS.gene041717:CDS | 45.0% |
| !! | GAAAGTATTGCGAGTCTTCG+AGG | + | chr6.3:70820475-70820494 | MS.gene041717:CDS | 45.0% |
| GGCAACAAAGGGAAGAATGG+TGG | + | chr6.3:70820819-70820838 | MS.gene041717:CDS | 50.0% | |
| !! | GGTTCATGAGGTTGGTGTCA+TGG | + | chr6.3:70820449-70820468 | MS.gene041717:CDS | 50.0% |
| !! | TGGTGGTGAAGGGTTAGCTT+TGG | + | chr6.3:70820836-70820855 | MS.gene041717:CDS | 50.0% |
| CAAAGAGCTTGCGTCCGAGT+CGG | + | chr6.3:70820608-70820627 | MS.gene041717:CDS | 55.0% | |
| GTGTGTGCTGAAGATGAGGG+AGG | + | chr6.3:70820665-70820684 | MS.gene041717:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 70820387 | 70821016 | 70820387 | ID=MS.gene041717 |
| chr6.3 | mRNA | 70820387 | 70821016 | 70820387 | ID=MS.gene041717.t1;Parent=MS.gene041717 |
| chr6.3 | exon | 70820387 | 70821016 | 70820387 | ID=MS.gene041717.t1.exon1;Parent=MS.gene041717.t1 |
| chr6.3 | CDS | 70820387 | 70821016 | 70820387 | ID=cds.MS.gene041717.t1;Parent=MS.gene041717.t1 |
| Gene Sequence |
| Protein sequence |
