Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92477.t1 | RHN52506.1 | 63.2 | 209 | 14 | 3 | 1 | 146 | 162 | 370 | 1.60E-56 | 228.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92477.t1 | A0A396HGY0 | 63.2 | 209 | 14 | 3 | 1 | 146 | 162 | 370 | 1.2e-56 | 228.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene92477 | 0.812267 | 4.62E-51 | -1.69E-46 |
| MS.gene049159 | MS.gene92477 | 0.84546 | 4.01E-59 | -1.69E-46 |
| MS.gene049435 | MS.gene92477 | 0.813237 | 2.83E-51 | -1.69E-46 |
| MS.gene049821 | MS.gene92477 | 0.805926 | 1.05E-49 | -1.69E-46 |
| MS.gene049850 | MS.gene92477 | 0.807009 | 6.23E-50 | -1.69E-46 |
| MS.gene050020 | MS.gene92477 | 0.83473 | 2.53E-56 | -1.69E-46 |
| MS.gene050442 | MS.gene92477 | 0.816786 | 4.62E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene92477 | 0.823199 | 1.58E-53 | -1.69E-46 |
| MS.gene051198 | MS.gene92477 | 0.81295 | 3.28E-51 | -1.69E-46 |
| MS.gene05157 | MS.gene92477 | 0.814592 | 1.42E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene92477 | 0.819967 | 8.79E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene92477 | 0.816376 | 5.71E-52 | -1.69E-46 |
| MS.gene052020 | MS.gene92477 | 0.801596 | 8.36E-49 | -1.69E-46 |
| MS.gene052611 | MS.gene92477 | 0.814012 | 1.91E-51 | -1.69E-46 |
| MS.gene052665 | MS.gene92477 | 0.815787 | 7.73E-52 | -1.69E-46 |
| MS.gene052863 | MS.gene92477 | 0.830819 | 2.37E-55 | -1.69E-46 |
| MS.gene052896 | MS.gene92477 | 0.807045 | 6.12E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene92477 | 0.813111 | 3.02E-51 | -1.69E-46 |
| MS.gene054472 | MS.gene92477 | 0.804011 | 2.65E-49 | -1.69E-46 |
| MS.gene05462 | MS.gene92477 | 0.801491 | 8.78E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene92477 | 0.817588 | 3.05E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene92477 | 0.81425 | 1.69E-51 | -1.69E-46 |
| MS.gene055926 | MS.gene92477 | 0.811265 | 7.63E-51 | -1.69E-46 |
| MS.gene057693 | MS.gene92477 | 0.80547 | 1.31E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene92477 | 0.827308 | 1.68E-54 | -1.69E-46 |
| MS.gene057915 | MS.gene92477 | 0.823137 | 1.63E-53 | -1.69E-46 |
| MS.gene058010 | MS.gene92477 | 0.816962 | 4.22E-52 | -1.69E-46 |
| MS.gene058636 | MS.gene92477 | 0.801765 | 7.72E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene92477 | 0.807307 | 5.39E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene92477 | 0.814212 | 1.73E-51 | -1.69E-46 |
| MS.gene059937 | MS.gene92477 | 0.805784 | 1.13E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene92477 | 0.835663 | 1.47E-56 | -1.69E-46 |
| MS.gene060998 | MS.gene92477 | 0.802186 | 6.32E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene92477 | MS.gene42493 | PPI |
| MS.gene041717 | MS.gene92477 | PPI |
| MS.gene92477 | MS.gene40432 | PPI |
| MS.gene92477 | MS.gene05157 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92477.t1 | MTR_6g081040 | 77.885 | 104 | 22 | 1 | 44 | 146 | 224 | 327 | 1.22e-41 | 141 |
| MS.gene92477.t1 | MTR_6g081040 | 86.957 | 46 | 5 | 1 | 1 | 45 | 119 | 164 | 2.19e-18 | 80.1 |
| MS.gene92477.t1 | MTR_6g081040 | 77.885 | 104 | 22 | 1 | 44 | 146 | 267 | 370 | 3.66e-41 | 140 |
| MS.gene92477.t1 | MTR_6g081040 | 86.957 | 46 | 5 | 1 | 1 | 45 | 162 | 207 | 3.34e-18 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTCCACTTAAACCTTCT+AGG | 0.230863 | 7.3:-76756537 | None:intergenic |
| AAAAGAGAGAGTCAAGAAAA+TGG | 0.291854 | 7.3:+76756444 | MS.gene92477:CDS |
| TTCAAATGGAGAAGCCTAGA+AGG | 0.411732 | 7.3:+76756523 | MS.gene92477:CDS |
| TCAAGGGTAGATACATGGTT+TGG | 0.416509 | 7.3:+76756386 | MS.gene92477:intron |
| GAAAGTATTGCGAGTCTTCG+AGG | 0.458677 | 7.3:+76756133 | MS.gene92477:CDS |
| TGGCGGTGAAGGCATAGCTT+TGG | 0.472745 | 7.3:+76756491 | MS.gene92477:CDS |
| TTTGGAAGTTCATGTTCAAA+TGG | 0.473753 | 7.3:+76756509 | MS.gene92477:CDS |
| GGAAGAAAGGTCATAGTTTG+TGG | 0.489302 | 7.3:+76756592 | MS.gene92477:CDS |
| AGATACATGGTTTGGAGAAG+AGG | 0.492157 | 7.3:+76756394 | MS.gene92477:intron |
| AGGTTTAAGTGGAAAGAAGA+AGG | 0.501263 | 7.3:+76756543 | MS.gene92477:CDS |
| GGTTCATGAGGTTGGTGTCA+TGG | 0.507161 | 7.3:+76756107 | MS.gene92477:CDS |
| AAGAAGAAGGAAATTCATCT+AGG | 0.520316 | 7.3:+76756556 | MS.gene92477:CDS |
| AAAGGGAAGAATGGCGGTGA+AGG | 0.530751 | 7.3:+76756480 | MS.gene92477:CDS |
| AGTCAAGAAAATGGTTGTAT+CGG | 0.537463 | 7.3:+76756453 | MS.gene92477:CDS |
| GATCTTCAAGGGTAGATACA+TGG | 0.550147 | 7.3:+76756381 | MS.gene92477:intron |
| AATGGTTGTATCGGCAACAA+AGG | 0.550602 | 7.3:+76756462 | MS.gene92477:CDS |
| AGAAGCCTAGAAGGTTTAAG+TGG | 0.552094 | 7.3:+76756532 | MS.gene92477:CDS |
| GATCAAGATCAAGCGCAAGT+TGG | 0.564528 | 7.3:-76756657 | None:intergenic |
| ATCGGCAACAAAGGGAAGAA+TGG | 0.569240 | 7.3:+76756471 | MS.gene92477:CDS |
| ATGGTTGTATCGGCAACAAA+GGG | 0.570595 | 7.3:+76756463 | MS.gene92477:CDS |
| CAAAGAGCTTGCTTCCGAGT+CGG | 0.577892 | 7.3:+76756268 | MS.gene92477:CDS |
| ACATGGACTTGTTGCTGTGT+GGG | 0.595042 | 7.3:+76756628 | MS.gene92477:CDS |
| TCTCTTAATGAAAACCGACT+CGG | 0.617722 | 7.3:-76756282 | None:intergenic |
| AATTGCTTCAACATCAACCA+TGG | 0.625609 | 7.3:-76756043 | None:intergenic |
| AACATGGACTTGTTGCTGTG+TGG | 0.658532 | 7.3:+76756627 | MS.gene92477:CDS |
| GGAACAAGGAGTATTATGAG+AGG | 0.665037 | 7.3:+76756415 | MS.gene92477:CDS |
| GGCAACAAAGGGAAGAATGG+CGG | 0.694360 | 7.3:+76756474 | MS.gene92477:CDS |
| TGGTTTGGAGAAGAGGAACA+AGG | 0.704148 | 7.3:+76756401 | MS.gene92477:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAATGTTATGTGTGATTTTT+TGG | + | chr7.3:76756086-76756105 | MS.gene92477:CDS | 25.0% |
| AAAAGAGAGAGTCAAGAAAA+TGG | + | chr7.3:76756444-76756463 | MS.gene92477:CDS | 30.0% | |
| AAGAAGAAGGAAATTCATCT+AGG | + | chr7.3:76756556-76756575 | MS.gene92477:CDS | 30.0% | |
| AGTCAAGAAAATGGTTGTAT+CGG | + | chr7.3:76756453-76756472 | MS.gene92477:CDS | 30.0% | |
| ATCAGAGTTTATCTATTGCA+TGG | + | chr7.3:76756348-76756367 | MS.gene92477:intron | 30.0% | |
| GGAAAATTGAAGATCTTCAA+GGG | + | chr7.3:76756370-76756389 | MS.gene92477:intron | 30.0% | |
| TCAGAGTTTATCTATTGCAT+GGG | + | chr7.3:76756349-76756368 | MS.gene92477:intron | 30.0% | |
| TTTGGAAGTTCATGTTCAAA+TGG | + | chr7.3:76756509-76756528 | MS.gene92477:CDS | 30.0% | |
| ! | GTTTTCATTAAGAGAAAACC+CGG | + | chr7.3:76756290-76756309 | MS.gene92477:CDS | 30.0% |
| !! | GAGTTATAGAGTTGATAGTT+TGG | + | chr7.3:76756235-76756254 | MS.gene92477:intron | 30.0% |
| AGGTTTAAGTGGAAAGAAGA+AGG | + | chr7.3:76756543-76756562 | MS.gene92477:CDS | 35.0% | |
| GGGAAAATTGAAGATCTTCA+AGG | + | chr7.3:76756369-76756388 | MS.gene92477:intron | 35.0% | |
| GTGGAAAAAGTTGAAGAACA+TGG | + | chr7.3:76756611-76756630 | MS.gene92477:CDS | 35.0% | |
| TCTCTTAATGAAAACCGACT+CGG | - | chr7.3:76756285-76756304 | None:intergenic | 35.0% | |
| TCTTTCCACTTAAACCTTCT+AGG | - | chr7.3:76756540-76756559 | None:intergenic | 35.0% | |
| ! | AAGAATTTTGCTGGGAAGAA+AGG | + | chr7.3:76756579-76756598 | MS.gene92477:CDS | 35.0% |
| ! | CATCTAGGAAGAATTTTGCT+GGG | + | chr7.3:76756571-76756590 | MS.gene92477:CDS | 35.0% |
| ! | TCATCTAGGAAGAATTTTGC+TGG | + | chr7.3:76756570-76756589 | MS.gene92477:CDS | 35.0% |
| !! | GTTTTGTTGAATGAGAATGC+TGG | + | chr7.3:76756209-76756228 | MS.gene92477:intron | 35.0% |
| !!! | GATTTTTTGGTTCATGAGGT+TGG | + | chr7.3:76756099-76756118 | MS.gene92477:CDS | 35.0% |
| !!! | GTGTGATTTTTTGGTTCATG+AGG | + | chr7.3:76756095-76756114 | MS.gene92477:CDS | 35.0% |
| AGAAGCCTAGAAGGTTTAAG+TGG | + | chr7.3:76756532-76756551 | MS.gene92477:CDS | 40.0% | |
| GATCTTCAAGGGTAGATACA+TGG | + | chr7.3:76756381-76756400 | MS.gene92477:intron | 40.0% | |
| GGAAGAAAGGTCATAGTTTG+TGG | + | chr7.3:76756592-76756611 | MS.gene92477:CDS | 40.0% | |
| TAAGTGTGTGCTGAAGATGA+GGG | + | chr7.3:76756322-76756341 | MS.gene92477:intron | 40.0% | |
| TCAAGGGTAGATACATGGTT+TGG | + | chr7.3:76756386-76756405 | MS.gene92477:intron | 40.0% | |
| TTCAAATGGAGAAGCCTAGA+AGG | + | chr7.3:76756523-76756542 | MS.gene92477:CDS | 40.0% | |
| ! | AATGGTTGTATCGGCAACAA+AGG | + | chr7.3:76756462-76756481 | MS.gene92477:CDS | 40.0% |
| ! | AGATACATGGTTTGGAGAAG+AGG | + | chr7.3:76756394-76756413 | MS.gene92477:intron | 40.0% |
| ! | ATGGTTGTATCGGCAACAAA+GGG | + | chr7.3:76756463-76756482 | MS.gene92477:CDS | 40.0% |
| ! | GTTACAAAAGAGTTTGCTCG+AGG | + | chr7.3:76756166-76756185 | MS.gene92477:intron | 40.0% |
| !! | GGAACAAGGAGTATTATGAG+AGG | + | chr7.3:76756415-76756434 | MS.gene92477:CDS | 40.0% |
| ACACACTTAGCTAACTCACC+GGG | - | chr7.3:76756311-76756330 | None:intergenic | 45.0% | |
| ATCGGCAACAAAGGGAAGAA+TGG | + | chr7.3:76756471-76756490 | MS.gene92477:CDS | 45.0% | |
| CACACACTTAGCTAACTCAC+CGG | - | chr7.3:76756312-76756331 | None:intergenic | 45.0% | |
| CTAAGTGTGTGCTGAAGATG+AGG | + | chr7.3:76756321-76756340 | MS.gene92477:intron | 45.0% | |
| ! | AAAGAGTTTGCTCGAGGTGT+TGG | + | chr7.3:76756172-76756191 | MS.gene92477:intron | 45.0% |
| ! | TGGTTTGGAGAAGAGGAACA+AGG | + | chr7.3:76756401-76756420 | MS.gene92477:CDS | 45.0% |
| !! | AACATGGACTTGTTGCTGTG+TGG | + | chr7.3:76756627-76756646 | MS.gene92477:CDS | 45.0% |
| !! | ACATGGACTTGTTGCTGTGT+GGG | + | chr7.3:76756628-76756647 | MS.gene92477:CDS | 45.0% |
| !! | GAAAGTATTGCGAGTCTTCG+AGG | + | chr7.3:76756133-76756152 | MS.gene92477:CDS | 45.0% |
| AAAGGGAAGAATGGCGGTGA+AGG | + | chr7.3:76756480-76756499 | MS.gene92477:CDS | 50.0% | |
| GGCAACAAAGGGAAGAATGG+CGG | + | chr7.3:76756474-76756493 | MS.gene92477:CDS | 50.0% | |
| ! | CAAAGAGCTTGCTTCCGAGT+CGG | + | chr7.3:76756268-76756287 | MS.gene92477:CDS | 50.0% |
| !! | GGTTCATGAGGTTGGTGTCA+TGG | + | chr7.3:76756107-76756126 | MS.gene92477:CDS | 50.0% |
| GTGTGTGCTGAAGATGAGGG+AGG | + | chr7.3:76756325-76756344 | MS.gene92477:intron | 55.0% | |
| ! | TGGCGGTGAAGGCATAGCTT+TGG | + | chr7.3:76756491-76756510 | MS.gene92477:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 76756045 | 76756671 | 76756045 | ID=MS.gene92477 |
| chr7.3 | mRNA | 76756045 | 76756671 | 76756045 | ID=MS.gene92477.t1;Parent=MS.gene92477 |
| chr7.3 | exon | 76756045 | 76756161 | 76756045 | ID=MS.gene92477.t1.exon1;Parent=MS.gene92477.t1 |
| chr7.3 | CDS | 76756045 | 76756161 | 76756045 | ID=cds.MS.gene92477.t1;Parent=MS.gene92477.t1 |
| chr7.3 | exon | 76756264 | 76756311 | 76756264 | ID=MS.gene92477.t1.exon2;Parent=MS.gene92477.t1 |
| chr7.3 | CDS | 76756264 | 76756311 | 76756264 | ID=cds.MS.gene92477.t1;Parent=MS.gene92477.t1 |
| chr7.3 | exon | 76756396 | 76756671 | 76756396 | ID=MS.gene92477.t1.exon3;Parent=MS.gene92477.t1 |
| chr7.3 | CDS | 76756396 | 76756671 | 76756396 | ID=cds.MS.gene92477.t1;Parent=MS.gene92477.t1 |
| Gene Sequence |
| Protein sequence |
