Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044441.t1 | XP_013457979.1 | 98.2 | 510 | 9 | 0 | 1 | 510 | 1 | 510 | 1.30E-289 | 1005 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044441.t1 | Q9SBQ9 | 68.5 | 508 | 156 | 3 | 5 | 510 | 6 | 511 | 6.5e-202 | 704.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044441.t1 | A0A072V1L1 | 98.2 | 510 | 9 | 0 | 1 | 510 | 1 | 510 | 9.2e-290 | 1005.0 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| No data available in table | ||||
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene001721 | MS.gene044441 | PPI |
| MS.gene005830 | MS.gene044441 | PPI |
| MS.gene009898 | MS.gene044441 | PPI |
| MS.gene033410 | MS.gene044441 | PPI |
| MS.gene033413 | MS.gene044441 | PPI |
| MS.gene04038 | MS.gene044441 | PPI |
| MS.gene044441 | MS.gene62831 | PPI |
| MS.gene044441 | MS.gene011458 | PPI |
| MS.gene044441 | MS.gene011456 | PPI |
| MS.gene044441 | MS.gene04563 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044441.t1 | MTR_4g109470 | 98.235 | 510 | 9 | 0 | 1 | 510 | 1 | 510 | 0.0 | 1035 |
| MS.gene044441.t1 | MTR_3g025230 | 75.292 | 514 | 120 | 4 | 1 | 509 | 1 | 512 | 0.0 | 772 |
| MS.gene044441.t1 | MTR_3g024520 | 75.292 | 514 | 120 | 4 | 1 | 509 | 1 | 512 | 0.0 | 772 |
| MS.gene044441.t1 | MTR_3g025260 | 71.206 | 514 | 141 | 4 | 1 | 509 | 1 | 512 | 0.0 | 748 |
| MS.gene044441.t1 | MTR_3g436540 | 49.225 | 516 | 241 | 8 | 6 | 510 | 9 | 514 | 7.50e-179 | 513 |
| MS.gene044441.t1 | MTR_3g436390 | 49.395 | 496 | 226 | 9 | 23 | 510 | 28 | 506 | 1.27e-174 | 501 |
| MS.gene044441.t1 | MTR_3g025250 | 64.939 | 328 | 67 | 5 | 184 | 509 | 1 | 282 | 2.75e-147 | 423 |
| MS.gene044441.t1 | MTR_8g020940 | 42.650 | 483 | 265 | 4 | 31 | 509 | 36 | 510 | 5.82e-147 | 431 |
| MS.gene044441.t1 | MTR_8g020950 | 42.443 | 483 | 266 | 4 | 31 | 509 | 38 | 512 | 5.83e-143 | 421 |
| MS.gene044441.t1 | MTR_8g020960 | 40.430 | 512 | 292 | 6 | 3 | 509 | 9 | 512 | 1.66e-137 | 407 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044441.t1 | AT5G07990 | 64.522 | 513 | 170 | 4 | 4 | 509 | 1 | 508 | 0.0 | 711 |
| MS.gene044441.t1 | AT2G45550 | 38.750 | 480 | 271 | 7 | 31 | 500 | 38 | 504 | 1.20e-118 | 358 |
| MS.gene044441.t1 | AT2G45560 | 37.917 | 509 | 291 | 8 | 4 | 500 | 9 | 504 | 3.80e-117 | 355 |
| MS.gene044441.t1 | AT2G40890 | 37.958 | 519 | 294 | 8 | 1 | 509 | 1 | 501 | 6.85e-117 | 354 |
| MS.gene044441.t1 | AT5G06900 | 37.800 | 500 | 288 | 11 | 5 | 500 | 9 | 489 | 6.94e-117 | 354 |
| MS.gene044441.t1 | AT4G12300 | 39.147 | 516 | 294 | 8 | 1 | 510 | 15 | 516 | 2.16e-114 | 348 |
| MS.gene044441.t1 | AT3G26310 | 38.716 | 483 | 277 | 7 | 28 | 505 | 30 | 498 | 4.41e-114 | 347 |
| MS.gene044441.t1 | AT3G61040 | 38.901 | 473 | 272 | 7 | 32 | 500 | 34 | 493 | 1.41e-113 | 345 |
| MS.gene044441.t1 | AT1G01280 | 38.298 | 517 | 296 | 8 | 4 | 509 | 5 | 509 | 1.73e-113 | 345 |
| MS.gene044441.t1 | AT4G12320 | 36.673 | 529 | 305 | 7 | 1 | 510 | 1 | 518 | 6.07e-113 | 344 |
Find 159 sgRNAs with CRISPR-Local
Find 316 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCAGAAAGGTTTCTTCC+TGG | 0.393453 | 8.1:-16161302 | MS.gene044441:CDS |
| AAACGCCACCGTGGTCCATA+AGG | 0.473854 | 8.1:+16166043 | None:intergenic |
| AAAGCTGCTAGGGCATGGTG+TGG | 0.671786 | 8.1:+16166244 | None:intergenic |
| AAATGAGTTGGACACTGTTG+TGG | 0.569675 | 8.1:-16161552 | MS.gene044441:CDS |
| AACAGCAAGCACAACAGAGT+GGG | 0.612112 | 8.1:-16161621 | MS.gene044441:CDS |
| AACCCCTCTATCCCTCCCAC+GGG | 0.572144 | 8.1:-16161441 | MS.gene044441:CDS |
| AACCCGTGGGAGGGATAGAG+GGG | 0.611184 | 8.1:+16161439 | None:intergenic |
| AACTTTGTGTTGCAACCCGT+GGG | 0.549176 | 8.1:+16161426 | None:intergenic |
| AAGTCCTGAAAAGCTCAATA+TGG | 0.422165 | 8.1:-16161117 | MS.gene044441:CDS |
| AAGTTTGATGTTGATGTTAG+GGG | 0.655910 | 8.1:-16161272 | MS.gene044441:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGCAGTACAGTACTAAAA+CGG | - | chr8.1:16163514-16163533 | MS.gene044441:intron | 30.0% | |
| AAGTTTGATGTTGATGTTAG+GGG | - | chr8.1:16166153-16166172 | MS.gene044441:CDS | 30.0% | |
| AGAAGTTTGATGTTGATGTT+AGG | - | chr8.1:16166151-16166170 | MS.gene044441:CDS | 30.0% | |
| AGTAATGAATCATGTTGTCA+AGG | - | chr8.1:16163036-16163055 | MS.gene044441:intron | 30.0% | |
| CAAAAATGCACATATAATGC+AGG | - | chr8.1:16164590-16164609 | MS.gene044441:intron | 30.0% | |
| CATAACTACATGAAAACGAA+AGG | + | chr8.1:16161498-16161517 | None:intergenic | 30.0% | |
| CCTCTATATATATGTTTGGT+CGG | + | chr8.1:16162348-16162367 | None:intergenic | 30.0% | |
| CTCTATATATATGTTTGGTC+GGG | + | chr8.1:16162347-16162366 | None:intergenic | 30.0% | |
| GAAAGTTGAGTAATACTTCA+GGG | - | chr8.1:16163940-16163959 | MS.gene044441:intron | 30.0% | |
| GAAGTTTGATGTTGATGTTA+GGG | - | chr8.1:16166152-16166171 | MS.gene044441:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 16161031 | 16166416 | 16161031 | ID=MS.gene044441 |
| chr8.1 | mRNA | 16161031 | 16166416 | 16161031 | ID=MS.gene044441.t1;Parent=MS.gene044441 |
| chr8.1 | exon | 16165973 | 16166416 | 16165973 | ID=MS.gene044441.t1.exon1;Parent=MS.gene044441.t1 |
| chr8.1 | CDS | 16165973 | 16166416 | 16165973 | ID=cds.MS.gene044441.t1;Parent=MS.gene044441.t1 |
| chr8.1 | exon | 16162386 | 16162835 | 16162386 | ID=MS.gene044441.t1.exon2;Parent=MS.gene044441.t1 |
| chr8.1 | CDS | 16162386 | 16162835 | 16162386 | ID=cds.MS.gene044441.t1;Parent=MS.gene044441.t1 |
| chr8.1 | exon | 16161031 | 16161669 | 16161031 | ID=MS.gene044441.t1.exon3;Parent=MS.gene044441.t1 |
| chr8.1 | CDS | 16161031 | 16161669 | 16161031 | ID=cds.MS.gene044441.t1;Parent=MS.gene044441.t1 |
| Gene Sequence |
>MS.gene044441 ATGTCTCCATTGATTATTGTCTTTGCTACAATTGCAGCAGCGATTCTCATTTACCGTCTCTTAAACCTAATAACCAAACCTTCCTTACCTCTTCCACCTGGGCCAACCCCATGGCCCATCGTCGGAAACCTACCTCACATGGGCCCAGTACCACACCATGCCCTAGCAGCTTTGGCCCTTAAACACGGTCCTCTCATGCATCTTCGTCTGGGCTACGTCAACGTGATTGTCGCTGCTTCCGCCTCCGTTGCTGAACAATTCTTAAAGGTTCATGATACCAATTTCTCTAGCCGGCCAACTAACTCCGGCGCCAAATACTTAGCATATAATTATCAAGATCTTGTGTTTGCTCCTTATGGACCACGGTGGCGTTTGTTAAGGAAAATATCGTACGTTCACATGTTCTCTTCTAAGGCTTTGGATGAATTTAGACACATACGCCAGGTAAGGATCATTTTTCGTTTCCTTTCGTTTTCATGTAGTTATGTTCATATTTTGTCTTTAAATCAACAATGTATTTTCATGCCATGGTACCCTACCACTTTACCCCTGCCTACTATTTTTTGACAGGTCCTCCTACTCTCCTTAAACAGCAAAAATAGCGGACAATTTATTATTCTCCCCTCCACTTTTGTACGTGAAGGGCTAGAATGGGGTCCATTTTTTACAAATGTTTTACAAAATTAAGTAGAAGTTGGTTTACTATAAATTATTTTAAAAAAATAGTATCAAATTGTTTTATAGGAAAGTTACCCAAAACAATTTTTAGATTTTTCTCCCTTCAAATTCTTGTTTGTTAATAAAACTTTAACAAATAGATGAAATATTCTAACAATGATTATATTATTTAAAAAAGTCATTTTTCTCGAAAAAACTTCAACAACCGGGATCTTAGATTGTAAAAATACATTTTTTTATACGTAGGAAATATCAATTGTATTTGTAAAAAAATTGGTAACGTATATTCATGAGTAGAATTTATAAGCTATTGCAAACTGTGTAGTCAATATAGAGATAATAAAATAAGGGTGCCGCTAACTAGTGTTTCTGAGGCACTGGTTAAGGAAACAAAAAAAAAAAAACAATAATTTTGTATTGAAAACAATATTTTCTACACTTTTCAATACTTACTACATTTGTTTTCTTAATTAGTGTCCCGGCGACACTAGTTAACATTTTCCTTAAAATAATTCGGTTTATGAAGTCTTCCATTTGAAAGGTAGCTCAACATCATGTGTAAAAATGAAATTGCTTATTACCAATTCTAGGATTATTGTCATAGTGAGTTTAAAAAGGACATAAAGAGAGGAAAGCCCGACCAAACATATATATAGAGGCCTAAATTAAAAATTAAAGGGGTTTTAAATATAATTAGCTTTTACTCTCTTCGTCCCTAATTATAGGACAATTTGCAAAAAATTGTTTATCCGTATATAAATATAAGTCTATAAATTTCTAAATGCGTTTATTATATTTTTCTCAAATATTTGTCTTTTTTCGAAGAACTATCAACTTGTCTTAAAATATAAAAAAAAATATTGAATGCATTTATATACAAATGGTAATTTAGTAGTTAAAACACACAATTAAAACAAATTTTTACTTCAACAACTTTACTTAATACTTATGATGACTTTTGTTACGTGTTCTTGTAAAAAGAAACTAATGGAGTAATATAATCTTAAAAAACACAGAAATATTTAGTGCATTTGCATTTGATCAATTAGTAGCAAAATTAATGAGTAAACTTCATACCATTTTTTTTAGAGAAATTGAGCTATCTTTTAGCTTATGTGTGAACTTGTGCGTGTGTGTTTTTTATGTGAACTAGTGTATTTTAAGTGATGTTTAATGCAACTCTTTTTTTTTAAGGATGTTTAATACAATTCTTTTTTTGAGAAAATGTTTAATACAATTTTTCAAAAATAGTTTTAAACAATAGTTTTGAATTCGACATTTCAAAAATACGGTATAATTAATTCCCATTTTACAACTGTTTATGATAAGTAATGAATCATGTTGTCAAGGTTACAAAGGGTGTGTTTGGTAAAAATAAGCTATAAGCTAGCTGATAGCTGATAGCTGAAAAGCTAGCTTATAGCTGATGGCTGATAGCTGATAGCTTATAGCTGAAAAGCTAGCTTATTAAAATTAAAAGTGTTTGGTAAAATTAGCTGTTGAAGTGACTGATAAATATAAAATGACATAAAAGGACATGTTTGTTTAATTTTTTTAATATCATATTTACAATTCTTATTACTTAATGTATTTCTTTTTAAATATTTAAATTTATTTCAAATTAATTTAATCATTAAAATGATAAATATATTTATGCATTTGACATTTTTTATTTAACAAAACTTTTTTTATAAATTTTATTAAACTAAACTTGCTTCAGTATTTATGGTAAATTTTATATAAAATTATTTATCATATGAATTAAGGGCAAGGTCAAAAAGAAAACTTGAACAAGAAAAGTTAAAAAAGCAGTACAGTACTAAAACGGAATGCTTGAACAAGTTCATCTTGTTTTTTGTTTTGAAGAGGAACGTGGGTGGTTATATTAGAAAGCGGTTAGTTTTTTTTAATTAAAAACAATAAATTAAAATAAATAAAAGGTTAAAATTGGAAGAAAATATAAAAAGCTATCAGCTATAAGCTAAAAAGCTACTTGAATTAGCTTCTTTAAAAACGCTATAAGCTAGTGAGAAAAGCTTTTTACCAAACACATGACATTTTATTAAAACAAGCTTATAAGCTAGTCCAACAAGCTATAAGCTAGCTTATTTGTGTTACCAAACACAGCCAAAGTTGTACTCTTAAGTTAACAACTCTTGAACAAATTCAATTGGAAAATCCAGTAAAAACAGTTGTTTGAAAATTATGTGTAGAATATGATTGACTATTTTGAAAGTTGAGTAATACTTCAGGGTGTTTCCAACATGGACCTCTGAAAACAACAGTATGAAGGCGTGTCTTTGCCATTTATAGTAAAAATGAATATTTCCCTAAAAAAAAAAAGTAAAAATGAATATTATAACAACAAAAAATGGAAAACTGAAATAATAATATTTTTTAAAACTTACCAAAAGTAATATGATCCCCCATTGGAACAAGTGCATCTTCCGGCGGAATACTTACTTCATTCAGTCTTTAACATGAGTAGAATTTAACAATATTAATAACTACCGTTAGAATTTTAGTTGGAACAAGTGCAGCTTTGGACCGAATTTTAAATTATCGACCCTTTTCTCATAGAATTAGATGTGTTTAATTGAGGCGTCATTTCCTCACACTCACCCTAAATTTTCCGTTACAAAATACTACAAAATAAAATTGTTTTTTTTTTGTTTGGTTTAGGTGATAAATAAAATTGTTAAGCGATAAAATATAGACTTTTTGGAGTACAAATTTGTGCTTGATAAATAATTATATTAGTTTTTATTTTATTTTATTTTAAGAAAAGCATGTAAATGAATTAATGTACAGTCGTTTGCAAACTTAAACATTTTATATATTTGTAAAGCAATTTAGATTAAAAAAAAACAATTTGAATTAACAAAAATGCACATATAATGCAGGAAGAGGTAGCAAGATTGATCCGCAATTTGGCAAGTTCAGGGTCCAAAGCAGTAAACTTAGGACAAATGTTGAATGTATGCACGACAAACGCATTGGCTAGGGTAATGATAGGACGGCGAGTATTTAATGAGGGGAATGGTGGGTGTGAATGTGATCCTAGGGCTGATGAGTTTAAGTCTATGGTGGTTGAACTTATGGTATTGGCTGGTGTTTTCAATATTGGTGATTTTATTCCTGCTTTGGAGTGGTTGGATCTTCAAGGTGTGCAAGGTAAGATGAAAAAATTACACAAAAGGTTTGATGCATTTTTAACAAGCATTATTGAGGACCATAGGATTTCTAAGAGTGAGAAGCACAATGATTTGTTGAGTACGTTGTTGTCACTCAAAGAAAAACTTCCTGAGGATGAAGACAAACTCAATGATACTGAGATCAAAGCATTACTCTTGGTACGTGTACATTCTACTGTCCAATTAAATCTTGTTTGTTTGTTTTTTTCTTTTCTTTTTTTGATAAAAAGAATACTTTTAATTTGTTAAAAAAAAATTCATCTATTTATTCAAGCTTAAACGGACTGACGATAAACAATTTGTAATAATTCTGGAAATTTACGTAGTAATTGCGGAAAAGTCGTAATAATATCGATCATCTTAAAAGAATATCATTATTTAATTATAATAAAAAGAAAATAATGTTTTTTTAAGGAAAAATGAAAATAATGTTTTTTTTAAAGAAGATAAATGAAAATAATGTTTTTTTTTTGAATAAATAAATGAAAATAATGTTGTTCCCCGAAAAAATGAAAATAATGTTTACTTTACATGATTTTTTTTAACAAGATTATATTTTCTTTTGAAAGAAACAAAATTATATGTTCATACATGGTTTGAAATCTCTTTATTTAATGCGAAAAAATGAAACCAAACTCTTAATATTTATCCACTAATGACCATATAACATATAAAAATCCATATTAAATAATTTTTATTTTATTGTTTAACAAAGGTCGTCTGTTACTTTTTATGTGACAATCTATATATAAAAAAAAATGTGAATTTATTAATCTTTGATATAAAACTTTATAAAGTCAAATTATTTTCAATATAAATTAAAACATTAATGAACTTGTAATATTCATGAGAGTTCAACTTTTGTGTACTTGTAGAACTTGTTCACAGCAGGAACAGATACAACAGCAAGCACAACAGAGTGGGCAATTTCTGAACTAATAAAAAATCCAAAACTTATGGCTTGTATCCAAAATGAGTTGGACACTGTTGTGGGACGAGACAGATTGGTAACTGAACAAGACTTGACCAACCTTCCTTACTTAGAGGCTGTTGTGAAGGAAACATTTCGTCTCCATCCATCAACCCCTCTATCCCTCCCACGGGTTGCAACACAAAGTTGTGAGATCTTTAACTACCATATCCCTAAAGGTGCTACTCTATTGGTTAATGTTTGGGCCATATCACGTGACCCTAAAGAATGGTCTAACCCATTGGAATTCAAACCAGAAAGGTTTCTTCCTGGTGGTGAGAAGTTTGATGTTGATGTTAGGGGAAATGACTTTGAGGTGATACCTTTTGGTGCTGGACGTAGAATATGTGCTGGTATGAGTCTTGGTCTTAGGATGGTTCAACTTCTCACAGCAACGTTGGCTCATGCATATGATTGGGAGTTGGAGAATGGTCAAAGTCCTGAAAAGCTCAATATGGATGAAGCATATGGACTCACCTTACAAAGAGCAGTGCCTATTTTGGCCCACCCTCGTCCAAGGCTTTCACCACATTTGTACTTGTAA |
| Protein sequence |
>MS.gene044441.t1 MSPLIIVFATIAAAILIYRLLNLITKPSLPLPPGPTPWPIVGNLPHMGPVPHHALAALALKHGPLMHLRLGYVNVIVAASASVAEQFLKVHDTNFSSRPTNSGAKYLAYNYQDLVFAPYGPRWRLLRKISYVHMFSSKALDEFRHIRQEEVARLIRNLASSGSKAVNLGQMLNVCTTNALARVMIGRRVFNEGNGGCECDPRADEFKSMVVELMVLAGVFNIGDFIPALEWLDLQGVQGKMKKLHKRFDAFLTSIIEDHRISKSEKHNDLLSTLLSLKEKLPEDEDKLNDTEIKALLLNLFTAGTDTTASTTEWAISELIKNPKLMACIQNELDTVVGRDRLVTEQDLTNLPYLEAVVKETFRLHPSTPLSLPRVATQSCEIFNYHIPKGATLLVNVWAISRDPKEWSNPLEFKPERFLPGGEKFDVDVRGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLAHAYDWELENGQSPEKLNMDEAYGLTLQRAVPILAHPRPRLSPHLYL |
