AlfalfaGEDB | MS.gene44877

M. sativa cultivar XinJiangDaYe / MS.gene44877

KEGG: K15401
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005506 GO:0008150 GO:0008152 GO:0016491 GO:0016705 GO:0020037 GO:0043167 GO:0043169 GO:0046872 GO:0046906 GO:0046914 GO:0048037 GO:0055114 GO:0097159 GO:1901363
PFAM: PF00067.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44877.t1	XP_003615606.1	97	399	12	0	1	399	1	399	4.50E-226	793.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44877.t1	P48422	72.0	400	108	2	1	398	1	398	7.5e-169	594.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44877.t1	G7K6C6	97.0	399	12	0	1	399	1	399	3.3e-226	793.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049611	MS.gene44877	0.800376	1.48E-48	-1.69E-46
MS.gene050082	MS.gene44877	0.809023	2.32E-50	-1.69E-46
MS.gene050338	MS.gene44877	0.809249	2.08E-50	-1.69E-46
MS.gene050809	MS.gene44877	0.802537	5.35E-49	-1.69E-46
MS.gene051138	MS.gene44877	0.815428	9.29E-52	-1.69E-46
MS.gene051422	MS.gene44877	0.812697	3.72E-51	-1.69E-46
MS.gene051651	MS.gene44877	0.814822	1.27E-51	-1.69E-46
MS.gene05240	MS.gene44877	0.819825	9.48E-53	-1.69E-46
MS.gene052702	MS.gene44877	0.814357	1.61E-51	-1.69E-46
MS.gene053420	MS.gene44877	0.813037	3.13E-51	-1.69E-46
MS.gene053920	MS.gene44877	0.825464	4.62E-54	-1.69E-46
MS.gene054181	MS.gene44877	0.829776	4.26E-55	-1.69E-46
MS.gene054706	MS.gene44877	0.813446	2.55E-51	-1.69E-46
MS.gene054742	MS.gene44877	0.801966	7.02E-49	-1.69E-46
MS.gene055959	MS.gene44877	0.826317	2.90E-54	-1.69E-46
MS.gene056198	MS.gene44877	0.800093	1.69E-48	-1.69E-46
MS.gene056387	MS.gene44877	-0.807547	4.79E-50	-1.69E-46
MS.gene056590	MS.gene44877	0.830683	2.56E-55	-1.69E-46
MS.gene056591	MS.gene44877	0.828094	1.09E-54	-1.69E-46
MS.gene056688	MS.gene44877	0.817474	3.24E-52	-1.69E-46
MS.gene056697	MS.gene44877	0.859598	3.70E-63	-1.69E-46
MS.gene056748	MS.gene44877	0.805351	1.39E-49	-1.69E-46
MS.gene056856	MS.gene44877	0.840192	1.01E-57	-1.69E-46
MS.gene057544	MS.gene44877	0.830001	3.75E-55	-1.69E-46
MS.gene057549	MS.gene44877	0.818872	1.56E-52	-1.69E-46
MS.gene057717	MS.gene44877	0.818757	1.66E-52	-1.69E-46
MS.gene057768	MS.gene44877	0.806587	7.65E-50	-1.69E-46
MS.gene058067	MS.gene44877	0.845145	4.87E-59	-1.69E-46
MS.gene058068	MS.gene44877	0.828906	6.93E-55	-1.69E-46
MS.gene058093	MS.gene44877	0.805087	1.58E-49	-1.69E-46
MS.gene058099	MS.gene44877	0.829415	5.22E-55	-1.69E-46
MS.gene058268	MS.gene44877	0.818552	1.85E-52	-1.69E-46
MS.gene058269	MS.gene44877	0.804985	1.66E-49	-1.69E-46
MS.gene058382	MS.gene44877	0.803354	3.63E-49	-1.69E-46
MS.gene059069	MS.gene44877	0.812219	4.73E-51	-1.69E-46
MS.gene059070	MS.gene44877	0.812032	5.20E-51	-1.69E-46
MS.gene05926	MS.gene44877	0.838751	2.39E-57	-1.69E-46
MS.gene059315	MS.gene44877	0.82819	1.03E-54	-1.69E-46
MS.gene060405	MS.gene44877	0.828068	1.10E-54	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene94506	MS.gene44877	PPI
MS.gene44877	MS.gene044441	PPI
MS.gene001721	MS.gene44877	PPI
MS.gene005830	MS.gene44877	PPI
MS.gene44877	MS.gene97810	PPI
MS.gene44877	MS.gene000022	PPI
MS.gene44877	MS.gene056797	PPI
MS.gene59358	MS.gene44877	PPI
MS.gene019650	MS.gene44877	PPI
MS.gene063487	MS.gene44877	PPI
MS.gene79433	MS.gene44877	PPI
MS.gene44877	MS.gene79428	PPI
MS.gene000023	MS.gene44877	PPI
MS.gene44877	MS.gene033068	PPI
MS.gene065589	MS.gene44877	PPI
MS.gene79430	MS.gene44877	PPI
MS.gene44877	MS.gene94508	PPI
MS.gene97813	MS.gene44877	PPI
MS.gene60558	MS.gene44877	PPI
MS.gene84824	MS.gene44877	PPI
MS.gene44877	MS.gene87433	PPI
MS.gene94504	MS.gene44877	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44877.t1	MTR_5g070010	96.992	399	12	0	1	399	1	399	0.0	806
MS.gene44877.t1	MTR_3g463060	74.055	397	90	1	3	399	8	391	0.0	605
MS.gene44877.t1	MTR_8g030590	61.125	409	142	4	1	399	1	402	0.0	517
MS.gene44877.t1	MTR_8g098435	62.069	406	140	5	1	399	1	399	0.0	516
MS.gene44877.t1	MTR_1g037560	42.708	384	200	6	23	394	56	431	3.04e-98	303
MS.gene44877.t1	MTR_4g075110	40.488	410	210	6	7	397	47	441	1.85e-97	301
MS.gene44877.t1	MTR_1g037550	41.266	395	196	6	23	395	56	436	3.30e-92	288
MS.gene44877.t1	MTR_7g406990	41.758	364	187	5	30	381	46	396	3.64e-88	274
MS.gene44877.t1	MTR_4g119660	35.814	430	224	10	1	399	1	409	2.07e-82	261
MS.gene44877.t1	MTR_7g096340	37.968	374	208	7	27	397	35	387	6.99e-81	257
MS.gene44877.t1	MTR_5g019140	36.543	405	208	9	26	399	38	424	2.13e-80	256
MS.gene44877.t1	MTR_7g096320	36.772	378	205	9	28	397	37	388	4.80e-76	244
MS.gene44877.t1	MTR_2g086040	41.325	317	167	4	85	397	88	389	1.25e-74	241
MS.gene44877.t1	MTR_7g088330	37.221	403	220	10	8	397	12	394	1.03e-72	236
MS.gene44877.t1	MTR_7g029275	33.017	421	234	8	2	399	3	398	6.08e-71	231
MS.gene44877.t1	MTR_3g080580	35.620	379	213	8	31	397	40	399	4.91e-69	227
MS.gene44877.t1	MTR_1g070345	35.396	404	215	9	14	397	12	389	2.17e-68	224
MS.gene44877.t1	MTR_4g054920	38.182	330	174	5	85	397	91	407	2.52e-67	222
MS.gene44877.t1	MTR_7g033570	34.688	369	224	6	34	399	33	387	2.69e-67	221
MS.gene44877.t1	MTR_8g088470	34.688	369	225	6	34	399	29	384	3.71e-67	221
MS.gene44877.t1	MTR_7g033580	34.115	384	235	7	18	397	16	385	1.04e-66	220
MS.gene44877.t1	MTR_1g070365	35.809	377	205	9	31	397	40	389	1.47e-66	220
MS.gene44877.t1	MTR_1g060550	34.262	359	212	6	45	399	64	402	1.99e-64	214
MS.gene44877.t1	MTR_6g004590	30.216	417	252	10	1	394	3	403	1.96e-60	204
MS.gene44877.t1	MTR_6g004670	32.447	376	226	7	34	397	37	396	8.16e-60	202
MS.gene44877.t1	MTR_6g004600	30.216	417	252	10	1	394	3	403	2.16e-59	201
MS.gene44877.t1	MTR_8g035810	32.039	412	248	10	1	394	1	398	1.69e-58	197
MS.gene44877.t1	MTR_1g102220	35.185	324	186	7	87	397	84	396	3.71e-58	197
MS.gene44877.t1	MTR_6g004630	31.707	410	245	10	7	397	1	394	4.54e-58	197
MS.gene44877.t1	MTR_8g035810	32.039	412	248	10	1	394	1	398	4.97e-58	197
MS.gene44877.t1	MTR_8g035760	31.926	379	216	9	35	397	49	401	1.04e-56	194
MS.gene44877.t1	MTR_8g035780	34.259	324	194	7	87	397	84	401	4.98e-56	192
MS.gene44877.t1	MTR_8g035860	30.825	412	249	10	4	394	1	397	8.27e-56	191
MS.gene44877.t1	MTR_8g035810	31.738	397	239	10	1	379	1	383	3.59e-55	187
MS.gene44877.t1	MTR_8g035680	31.649	376	222	10	35	397	49	402	1.56e-54	188
MS.gene44877.t1	MTR_6g004610	31.880	367	227	7	40	397	2	354	3.24e-54	186
MS.gene44877.t1	MTR_1g036200	30.591	389	247	7	17	397	17	390	1.06e-53	186
MS.gene44877.t1	MTR_8g035820	33.542	319	197	6	87	394	83	397	1.46e-52	183
MS.gene44877.t1	MTR_1g035670	29.412	408	262	6	1	397	1	393	6.34e-52	181
MS.gene44877.t1	MTR_1g050362	30.637	408	249	10	10	397	5	398	3.59e-51	179
MS.gene44877.t1	MTR_1g036130	29.440	411	262	7	1	397	1	397	4.11e-51	179
MS.gene44877.t1	MTR_1g036270	30.052	386	233	8	34	397	33	403	1.94e-49	174
MS.gene44877.t1	MTR_1g035750	29.798	396	249	9	17	397	15	396	2.51e-49	174
MS.gene44877.t1	MTR_1g035790	30.831	373	237	8	34	397	33	393	3.48e-49	174
MS.gene44877.t1	MTR_1g035860	29.412	374	240	7	31	394	37	396	2.19e-46	166
MS.gene44877.t1	MTR_1g035760	30.597	402	242	8	1	397	1	370	8.03e-46	164
MS.gene44877.t1	MTR_1g036010	27.778	414	260	10	1	394	1	395	1.81e-45	164
MS.gene44877.t1	MTR_1g035830	30.105	382	238	10	31	397	31	398	3.21e-45	163
MS.gene44877.t1	MTR_1g035940	27.704	379	245	8	34	397	34	398	3.36e-43	157
MS.gene44877.t1	MTR_1g035800	29.870	385	235	9	34	397	33	403	3.81e-43	157
MS.gene44877.t1	MTR_1g035580	29.351	385	222	8	34	397	32	387	1.55e-42	155
MS.gene44877.t1	MTR_1g070370	37.118	229	118	5	177	397	5	215	2.09e-38	138
MS.gene44877.t1	MTR_1g069715	28.882	322	206	7	92	397	2	316	6.70e-36	139
MS.gene44877.t1	MTR_8g035680	31.301	246	146	7	165	397	20	255	1.48e-33	129
MS.gene44877.t1	MTR_2g062670	36.111	180	106	3	176	351	67	241	1.15e-29	120
MS.gene44877.t1	MTR_2g062670	30.357	168	77	4	176	343	283	410	3.92e-18	86.3
MS.gene44877.t1	MTR_2g061750	34.254	181	105	4	86	253	16	195	1.40e-24	100
MS.gene44877.t1	MTR_2g061740	34.254	181	105	4	86	253	16	195	1.53e-24	100
MS.gene44877.t1	MTR_1g102210	29.435	248	124	9	165	397	40	251	1.42e-20	92.8
MS.gene44877.t1	MTR_4g012320	43.750	112	56	2	284	394	6	111	1.42e-18	84.3
MS.gene44877.t1	MTR_7g079440	25.000	324	206	10	84	391	231	533	2.42e-18	87.4
MS.gene44877.t1	MTR_7g079440	25.000	324	206	10	84	391	231	533	2.76e-18	87.4
MS.gene44877.t1	MTR_8g046580	37.037	108	67	1	91	198	61	167	8.27e-16	77.8
MS.gene44877.t1	MTR_5g009110	22.812	377	235	14	50	399	96	443	8.94e-14	73.2
MS.gene44877.t1	MTR_5g009110	22.895	380	231	14	50	399	96	443	1.30e-13	72.4
MS.gene44877.t1	MTR_2g061910	40.741	81	48	0	86	166	73	153	1.69e-13	68.6
MS.gene44877.t1	MTR_4g088420	37.391	115	62	2	285	399	16	120	1.52e-12	67.4
MS.gene44877.t1	MTR_4g104660	22.867	293	190	8	123	399	131	403	2.71e-12	68.6
MS.gene44877.t1	MTR_2g065360	24.235	392	237	15	24	391	4	359	3.01e-12	68.2
MS.gene44877.t1	MTR_1g023040	32.051	156	67	5	189	343	14	131	6.48e-12	63.5
MS.gene44877.t1	MTR_3g436540	22.030	404	263	12	18	398	21	395	6.96e-12	67.4
MS.gene44877.t1	MTR_2g073480	24.123	228	155	6	178	398	86	302	8.77e-12	66.6
MS.gene44877.t1	MTR_5g073250	23.117	385	246	10	30	399	41	390	1.11e-11	66.6
MS.gene44877.t1	MTR_2g073440	24.123	228	155	6	178	398	153	369	1.35e-11	66.2
MS.gene44877.t1	MTR_8g012585	26.871	294	173	12	127	397	63	337	1.52e-11	66.2
MS.gene44877.t1	MTR_2g061760	34.694	98	61	2	158	253	5	101	2.64e-11	60.8
MS.gene44877.t1	MTR_3g465300	21.343	417	253	17	19	398	33	411	2.86e-11	65.5
MS.gene44877.t1	MTR_4g104540	29.530	149	91	4	253	399	185	321	3.08e-11	65.1
MS.gene44877.t1	MTR_5g009110	22.977	309	197	11	118	399	1	295	3.83e-11	64.7
MS.gene44877.t1	MTR_5g009110	22.977	309	197	11	118	399	1	295	6.42e-11	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44877.t1	AT5G58860	72.000	400	108	2	1	398	1	398	0.0	605
MS.gene44877.t1	AT4G00360	59.950	402	146	3	8	399	7	403	1.46e-176	504
MS.gene44877.t1	AT2G45970	59.804	408	148	4	1	399	1	401	2.02e-175	500
MS.gene44877.t1	AT1G63710	60.099	406	144	4	3	399	5	401	1.70e-172	492
MS.gene44877.t1	AT1G01600	59.158	404	148	4	8	399	7	405	7.73e-171	489
MS.gene44877.t1	AT5G23190	40.547	402	221	5	7	397	38	432	6.01e-98	303
MS.gene44877.t1	AT5G08250	42.933	375	191	7	33	395	3	366	9.21e-98	300
MS.gene44877.t1	AT5G08250	42.857	378	193	7	30	395	62	428	9.58e-98	302
MS.gene44877.t1	AT1G13140	39.737	380	209	6	30	397	45	416	7.56e-90	281
MS.gene44877.t1	AT1G13140	39.516	372	195	6	30	397	45	390	3.26e-84	266
MS.gene44877.t1	AT1G13150	38.010	392	220	6	21	397	26	409	5.93e-84	266
MS.gene44877.t1	AT1G24540	36.409	401	228	5	13	397	23	412	3.31e-82	261
MS.gene44877.t1	AT1G69500	35.267	431	230	8	4	399	1	417	3.53e-81	258
MS.gene44877.t1	AT3G26125	36.869	396	204	7	31	397	46	424	6.14e-80	256
MS.gene44877.t1	AT1G34540	39.096	376	203	9	30	397	30	387	8.47e-79	251
MS.gene44877.t1	AT3G56630	38.133	375	208	7	30	397	30	387	1.10e-78	251
MS.gene44877.t1	AT3G01900	36.658	371	209	8	31	399	32	378	5.00e-73	236
MS.gene44877.t1	AT3G48520	35.263	380	211	7	27	397	32	385	3.28e-68	224
MS.gene44877.t1	AT2G27690	41.066	319	170	7	85	397	76	382	2.47e-65	216
MS.gene44877.t1	AT4G39490	34.625	413	241	10	7	399	6	409	2.73e-65	217
MS.gene44877.t1	AT5G63450	33.333	411	232	8	1	395	1	385	1.92e-62	209
MS.gene44877.t1	AT2G45510	34.845	419	231	13	1	397	1	399	6.80e-62	207
MS.gene44877.t1	AT5G63450	33.333	411	232	8	1	395	59	443	7.13e-62	209
MS.gene44877.t1	AT2G23180	32.938	422	240	8	4	399	1	405	1.08e-60	204
MS.gene44877.t1	AT1G57750	32.775	418	233	11	1	399	1	389	1.62e-60	202
MS.gene44877.t1	AT1G57750	32.775	418	233	11	1	399	1	389	6.09e-60	202
MS.gene44877.t1	AT2G44890	33.816	414	238	12	1	397	1	395	1.45e-59	201
MS.gene44877.t1	AT2G44890	33.014	418	240	11	1	397	1	399	3.71e-59	201
MS.gene44877.t1	AT2G21910	31.332	383	229	7	31	397	32	396	6.84e-58	197
MS.gene44877.t1	AT4G32170	31.463	410	245	8	7	399	6	396	1.41e-57	196
MS.gene44877.t1	AT4G39480	34.595	370	209	10	49	399	51	406	2.41e-56	193
MS.gene44877.t1	AT5G52320	32.632	380	217	9	34	397	37	393	5.51e-56	192
MS.gene44877.t1	AT5G52320	32.632	380	217	9	34	397	37	393	6.33e-56	192
MS.gene44877.t1	AT1G65340	32.632	380	218	8	34	397	37	394	1.07e-55	191
MS.gene44877.t1	AT4G39500	32.117	411	240	10	10	399	17	409	7.32e-54	187
MS.gene44877.t1	AT4G39510	30.097	412	250	8	7	399	6	398	3.51e-53	184
MS.gene44877.t1	AT1G47620	29.657	408	248	10	14	399	18	408	4.73e-51	179
MS.gene44877.t1	AT5G02900	30.105	382	211	11	34	397	26	369	6.19e-48	170
MS.gene44877.t1	AT4G39500	31.649	376	223	8	40	399	2	359	7.17e-48	169
MS.gene44877.t1	AT4G37410	23.105	277	197	5	125	398	119	382	2.27e-17	84.3
MS.gene44877.t1	AT1G13110	23.990	421	267	14	4	399	1	393	9.12e-17	82.4
MS.gene44877.t1	AT4G37400	23.636	275	192	7	127	398	121	380	1.77e-14	75.5
MS.gene44877.t1	AT1G50560	26.957	230	143	8	165	385	174	387	6.57e-14	73.6
MS.gene44877.t1	AT1G31800	22.050	322	218	9	84	391	152	454	8.56e-14	73.6
MS.gene44877.t1	AT4G37340	24.221	417	265	13	1	398	1	385	3.88e-13	71.2
MS.gene44877.t1	AT3G26210	27.068	266	166	12	127	379	120	370	3.98e-12	68.2
MS.gene44877.t1	AT5G10610	27.675	271	159	10	123	379	118	365	8.19e-12	67.0
MS.gene44877.t1	AT4G37330	25.784	287	179	10	125	397	121	387	8.83e-12	67.0
MS.gene44877.t1	AT1G13080	24.567	289	191	10	123	398	116	390	2.19e-11	65.9
MS.gene44877.t1	AT4G15110	22.222	342	224	12	77	394	116	439	3.36e-11	65.5
MS.gene44877.t1	AT2G25160	33.835	133	76	3	259	389	269	391	3.59e-11	65.1
MS.gene44877.t1	AT2G25160	34.091	132	75	3	260	389	270	391	3.99e-11	65.1
MS.gene44877.t1	AT5G51900	27.097	155	94	3	223	359	1	154	5.52e-11	62.8

Find 116 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATGTATGTTTCTACAATT+TGG	0.165510	5.2:-59262619	None:intergenic
AAACCGACTATGGTGTATTT+TGG	0.174156	5.2:+59261727	MS.gene44877:CDS
TTGCTCTCAGCTGGTTCTTC+TGG	0.199506	5.2:+59262795	MS.gene44877:CDS
TTTGCCACGTAGGCCCTTTC+GGG	0.242244	5.2:-59261562	None:intergenic
AACTGCATTTCACGATCTTT+TGG	0.262319	5.2:+59261583	MS.gene44877:CDS
GTTTGCCACGTAGGCCCTTT+CGG	0.282214	5.2:-59261563	None:intergenic
CCGATGAAGGGCCATGCTTT+TGG	0.286678	5.2:-59261341	None:intergenic
CCCTCTCTGCCTATTTCCTT+TGG	0.287982	5.2:+59261288	MS.gene44877:CDS
AGGCAGAGAGGGAGGCTGTT+AGG	0.297332	5.2:-59261277	None:intergenic
AACGCCGGTGAAAGAGTTTC+AGG	0.297525	5.2:-59261845	None:intergenic
TCCAATCATGAACTCGGTTC+CGG	0.322013	5.2:-59261388	None:intergenic
TCCTCTACCCAGGTCTAATA+TGG	0.330952	5.2:+59262543	MS.gene44877:CDS
GCCCTTCATCGGTAGCCTTC+CGG	0.354269	5.2:+59261352	MS.gene44877:CDS
GGCCGGAAGGCTACCGATGA+AGG	0.369556	5.2:-59261354	None:intergenic
TGTAGCACTGTTGCGTTGAT+TGG	0.369771	5.2:-59262725	None:intergenic
CTTTCGGGTAATTATCGAAC+CGG	0.376839	5.2:-59261547	None:intergenic
GAAAAGGGATAATGCATGTT+TGG	0.376856	5.2:-59261451	None:intergenic
GGTTTCCTTGCGATCTGAAA+TGG	0.394738	5.2:-59262648	None:intergenic
TTAACATTTGATAATATTTG+TGG	0.398061	5.2:+59261803	MS.gene44877:CDS
GAGATGGAACCAAAGGAAAT+AGG	0.400112	5.2:-59261297	None:intergenic
CTCCTCGCCCGAACCCTAAC+CGG	0.401641	5.2:+59261317	MS.gene44877:CDS
CATAATCTTAAACTTCATCT+TGG	0.408457	5.2:+59262751	MS.gene44877:CDS
GGCAGAGAGGGAGGCTGTTA+GGG	0.409411	5.2:-59261276	None:intergenic
TCTCAGCTGGTTCTTCTGGC+TGG	0.416231	5.2:+59262799	MS.gene44877:CDS
CCTTTCTTGGCTCACAAGCA+AGG	0.416901	5.2:+59261473	MS.gene44877:CDS
ATGAAGAAACGAGACATAGA+TGG	0.425791	5.2:+59262698	MS.gene44877:CDS
ACCGGAACCGAGTTCATGAT+TGG	0.426606	5.2:+59261387	MS.gene44877:CDS
AATATTTGTGGACTTACGTT+AGG	0.428971	5.2:+59261815	MS.gene44877:CDS
CTTTCTTGGCTCACAAGCAA+GGG	0.444050	5.2:+59261474	MS.gene44877:CDS
AACCGCTGCCCTCGAATTCA+CGG	0.445492	5.2:+59261655	MS.gene44877:CDS
TTGTGAGCCAAGAAAGGAAA+AGG	0.445700	5.2:-59261467	None:intergenic
TTTCAGTGAAGCGGATCAAT+TGG	0.447152	5.2:+59262928	MS.gene44877:CDS
GGTTTATTTGAAAGCGGCAT+TGG	0.449392	5.2:+59262949	MS.gene44877:CDS
GCGGAAAATCTCCGAGCAAC+TGG	0.449775	5.2:+59261413	MS.gene44877:CDS
AACTGATGATGTGTCCCTTC+CGG	0.455158	5.2:-59262774	None:intergenic
CTATGGCTCGATGGGTGAAC+CGG	0.460543	5.2:+59261696	MS.gene44877:CDS
ATCTTAAACTTCATCTTGGC+CGG	0.464594	5.2:+59262755	MS.gene44877:CDS
CGCTGGAACAACTGTTCCAT+CGG	0.470965	5.2:-59263041	None:intergenic
TTAGGGTTCGGGCGAGGAGA+TGG	0.473448	5.2:-59261313	None:intergenic
ATGTTCGTGCCGTGAATTCG+AGG	0.476259	5.2:-59261664	None:intergenic
CATCATCAGTTGCTCTCAGC+TGG	0.486555	5.2:+59262786	MS.gene44877:CDS
AATTATCGAACCGGGTTCGA+AGG	0.492468	5.2:-59261538	None:intergenic
GTGTCAAAAGCAACAGAAAA+GGG	0.494022	5.2:-59261878	None:intergenic
TGCCGTGAATTCGAGGGCAG+CGG	0.495798	5.2:-59261657	None:intergenic
GACCGGTTAGGGTTCGGGCG+AGG	0.500245	5.2:-59261319	None:intergenic
CCGCGATCCAATCATGAACT+CGG	0.502112	5.2:-59261394	None:intergenic
GGTGTCAAAAGCAACAGAAA+AGG	0.504563	5.2:-59261879	None:intergenic
GGGTTCGAAGGATGTGTTCG+AGG	0.505106	5.2:-59261526	None:intergenic
CCTGTGCATGGTGGCTTCAG+TGG	0.515527	5.2:-59261903	None:intergenic
TTATTTGAAAGCGGCATTGG+CGG	0.519244	5.2:+59262952	MS.gene44877:CDS
AGAGGGAGGCTGTTAGGGTA+AGG	0.522124	5.2:-59261271	None:intergenic
CGTCGCTGTTGAAAATGCCT+TGG	0.524131	5.2:-59261607	None:intergenic
TTTGTATCCGTCAGTGCCGC+AGG	0.531175	5.2:+59262985	MS.gene44877:CDS
TGAAACTAGCTATGGCTCGA+TGG	0.532408	5.2:+59261687	MS.gene44877:CDS
GGATATCAAGCAGGCAGTCG+TGG	0.533881	5.2:+59263006	MS.gene44877:CDS
CCTTGCTTGTGAGCCAAGAA+AGG	0.534180	5.2:-59261473	None:intergenic
AGACATTAAGTCATCGGAAG+GGG	0.534696	5.2:-59262669	None:intergenic
TATCCAAAATACACCATAGT+CGG	0.536774	5.2:-59261730	None:intergenic
TGAAACCTCCATATTAGACC+TGG	0.538839	5.2:-59262551	None:intergenic
GGTTCGATAATTACCCGAAA+GGG	0.544252	5.2:+59261549	MS.gene44877:CDS
GCCGGAAGGCTACCGATGAA+GGG	0.550316	5.2:-59261353	None:intergenic
CCAAAGGAAATAGGCAGAGA+GGG	0.555146	5.2:-59261288	None:intergenic
CCCTAACCGGTCCAAAAGCA+TGG	0.557273	5.2:+59261330	MS.gene44877:CDS
TCCGCTTGATTTCAGTGAAG+CGG	0.558902	5.2:+59262919	MS.gene44877:CDS
GAAACTAGCTATGGCTCGAT+GGG	0.559504	5.2:+59261688	MS.gene44877:CDS
TTGATATCCTGCGGCACTGA+CGG	0.559723	5.2:-59262992	None:intergenic
TAAACTTCATCTTGGCCGGA+AGG	0.566371	5.2:+59262759	MS.gene44877:CDS
CCACTGAAGCCACCATGCAC+AGG	0.566415	5.2:+59261903	MS.gene44877:CDS
TGGCTGAACCACCAGTTGCT+CGG	0.569314	5.2:-59261424	None:intergenic
CGGTTCGATAATTACCCGAA+AGG	0.569958	5.2:+59261548	MS.gene44877:CDS
AGTTGAGGAGAAAATAATCA+AGG	0.570335	5.2:+59262838	MS.gene44877:CDS
GCTGGAACAACTGTTCCATC+GGG	0.572512	5.2:-59263040	None:intergenic
CCAAAAGCATGGCCCTTCAT+CGG	0.572585	5.2:+59261341	MS.gene44877:CDS
TCCATATTAGACCTGGGTAG+AGG	0.573226	5.2:-59262544	None:intergenic
CGAGACATTAAGTCATCGGA+AGG	0.574841	5.2:-59262671	None:intergenic
GAAACGAGACATTAAGTCAT+CGG	0.575285	5.2:-59262675	None:intergenic
TCAATTGGTTTATTTGAAAG+CGG	0.575733	5.2:+59262943	MS.gene44877:CDS
ACCAAAGGAAATAGGCAGAG+AGG	0.577966	5.2:-59261289	None:intergenic
AGACCCTGAAACTCTTTCAC+CGG	0.578867	5.2:+59261841	MS.gene44877:CDS
CAACGCCATTTCAGATCGCA+AGG	0.579512	5.2:+59262643	MS.gene44877:CDS
TGTGAGCCAAGAAAGGAAAA+GGG	0.581941	5.2:-59261466	None:intergenic
CGATGGAACAGTTGTTCCAG+CGG	0.593470	5.2:+59263042	MS.gene44877:CDS
TTTCGGGTAATTATCGAACC+GGG	0.595782	5.2:-59261546	None:intergenic
TCGAACACATCCTTCGAACC+CGG	0.597628	5.2:+59261528	MS.gene44877:CDS
GGAGTTAACCACTGTTCTTG+AGG	0.597985	5.2:+59262859	MS.gene44877:CDS
TCTACCCAGGTCTAATATGG+AGG	0.599018	5.2:+59262546	MS.gene44877:CDS
ACGCCGGTGAAAGAGTTTCA+GGG	0.603266	5.2:-59261844	None:intergenic
ACGAACATTGAAACTAGCTA+TGG	0.603699	5.2:+59261679	MS.gene44877:CDS
GATGGAACAGTTGTTCCAGC+GGG	0.604671	5.2:+59263043	MS.gene44877:CDS
TCCGCTTCACTGAAATCAAG+CGG	0.609099	5.2:-59262920	None:intergenic
AAACTTCATCTTGGCCGGAA+GGG	0.611766	5.2:+59262760	MS.gene44877:CDS
GAAACCTCCATATTAGACCT+GGG	0.611859	5.2:-59262550	None:intergenic
GGTTAAAGATAATGTCTATG+TGG	0.611909	5.2:+59261757	MS.gene44877:CDS
GGGCGAGGAGATGGAACCAA+AGG	0.616249	5.2:-59261304	None:intergenic
AGTGCCGCAGGATATCAAGC+AGG	0.616718	5.2:+59262997	MS.gene44877:CDS
TGTTCGTGCCGTGAATTCGA+GGG	0.624155	5.2:-59261663	None:intergenic
ATATATAGGTTCCTCTACCC+AGG	0.624510	5.2:+59262533	MS.gene44877:intron
GATAATGCATGTTTGGTATG+TGG	0.626077	5.2:-59261444	None:intergenic
TCAACAGCGACGGTGAAACG+TGG	0.626506	5.2:+59261618	MS.gene44877:CDS
CATGAACCATCCGAAAGTTG+AGG	0.626510	5.2:+59262823	MS.gene44877:CDS
TGAAATGCAGTTTGCCACGT+AGG	0.632446	5.2:-59261572	None:intergenic
GAAAATCTCCGAGCAACTGG+TGG	0.635625	5.2:+59261416	MS.gene44877:CDS
TTAGGGTAAGGAGAAGAGTG+AGG	0.652517	5.2:-59261259	None:intergenic
TAGGGTAAGGAGAAGAGTGA+GGG	0.653407	5.2:-59261258	None:intergenic
GTGCATGGTGGCTTCAGTGG+CGG	0.653546	5.2:-59261900	None:intergenic
AAGGAAATAGGCAGAGAGGG+AGG	0.655551	5.2:-59261285	None:intergenic
TGTCAAAAGCAACAGAAAAG+GGG	0.657811	5.2:-59261877	None:intergenic
GAGACATTAAGTCATCGGAA+GGG	0.658249	5.2:-59262670	None:intergenic
TCGAGTTTCCTCAAGAACAG+TGG	0.669542	5.2:-59262867	None:intergenic
AAGAGTTACTGACCTGTGCA+TGG	0.674124	5.2:-59261915	None:intergenic
ATTACCCGAAAGGGCCTACG+TGG	0.691464	5.2:+59261558	MS.gene44877:CDS
ACTGTTCTTGAGGAAACTCG+AGG	0.694261	5.2:+59262869	MS.gene44877:CDS
CCGAGTTCATGATTGGATCG+CGG	0.700241	5.2:+59261394	MS.gene44877:CDS
ACTGCCTGCTTGATATCCTG+CGG	0.704665	5.2:-59263001	None:intergenic
GTTCTTGAGGAAACTCGAGG+AGG	0.744109	5.2:+59262872	MS.gene44877:CDS
AGTTACTGACCTGTGCATGG+TGG	0.749084	5.2:-59261912	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATATAATATAATAAGAG+AGG	+	chr5.2:59262329-59262348	MS.gene44877:intron	10.0%
!!!	ATTTTAAAAATAAAGCAAAA+TGG	-	chr5.2:59262141-59262160	None:intergenic	10.0%
!!	AAATTCTGTCAATTATTAAA+CGG	-	chr5.2:59262392-59262411	None:intergenic	15.0%
!!!	AAAATGGTTTTTCTTTTTAA+GGG	-	chr5.2:59262125-59262144	None:intergenic	15.0%
!!!	ACTTTGTTTTAACATTTTTT+CGG	-	chr5.2:59262597-59262616	None:intergenic	15.0%
!!!	TTAACATTTGATAATATTTG+TGG	+	chr5.2:59261803-59261822	MS.gene44877:CDS	15.0%
!!	AATAATTGCTCAACATATAT+AGG	+	chr5.2:59262519-59262538	MS.gene44877:intron	20.0%
!!!	CAAAATGGTTTTTCTTTTTA+AGG	-	chr5.2:59262126-59262145	None:intergenic	20.0%
!	ACTCAACTAAAATATATGCT+TGG	-	chr5.2:59262365-59262384	None:intergenic	25.0%
!	CATAATCTTAAACTTCATCT+TGG	+	chr5.2:59262751-59262770	MS.gene44877:CDS	25.0%
!	TACTTATAGTTGTAGACATA+TGG	+	chr5.2:59261937-59261956	MS.gene44877:intron	25.0%
!!	ATTATTTTCTCCTCAACTTT+CGG	-	chr5.2:59262836-59262855	None:intergenic	25.0%
!!	TCAATTGGTTTATTTGAAAG+CGG	+	chr5.2:59262943-59262962	MS.gene44877:CDS	25.0%
!!	TCATGTATGTTTCTACAATT+TGG	-	chr5.2:59262622-59262641	None:intergenic	25.0%
!!!	ATGGTGTATTTTGGATAAAT+CGG	+	chr5.2:59261736-59261755	MS.gene44877:CDS	25.0%
!!!	ATTTTTGACAAGTTTTGTTC+AGG	-	chr5.2:59262270-59262289	None:intergenic	25.0%
!!!	TAAACATTTTAGTGATTAGC+TGG	-	chr5.2:59262198-59262217	None:intergenic	25.0%
	AATATTTGTGGACTTACGTT+AGG	+	chr5.2:59261815-59261834	MS.gene44877:CDS	30.0%
	AGTTGAGGAGAAAATAATCA+AGG	+	chr5.2:59262838-59262857	MS.gene44877:CDS	30.0%
	CTTATTGTGAAACGTGATTT+GGG	+	chr5.2:59261990-59262009	MS.gene44877:intron	30.0%
	GGTTAAAGATAATGTCTATG+TGG	+	chr5.2:59261757-59261776	MS.gene44877:CDS	30.0%
	TATCCAAAATACACCATAGT+CGG	-	chr5.2:59261733-59261752	None:intergenic	30.0%
!	TGGAGGTTTCAAAAACTTTT+CGG	+	chr5.2:59262563-59262582	MS.gene44877:CDS	30.0%
!	TTTCAAAAACTTTTCGGCAT+AGG	+	chr5.2:59262569-59262588	MS.gene44877:CDS	30.0%
	ACGAACATTGAAACTAGCTA+TGG	+	chr5.2:59261679-59261698	MS.gene44877:CDS	35.0%
	AGAAAAGCAAAAATGGACAG+AGG	+	chr5.2:59262895-59262914	MS.gene44877:CDS	35.0%
	ATCTTAAACTTCATCTTGGC+CGG	+	chr5.2:59262755-59262774	MS.gene44877:CDS	35.0%
	ATGAAGAAACGAGACATAGA+TGG	+	chr5.2:59262698-59262717	MS.gene44877:CDS	35.0%
	GAAAAGGGATAATGCATGTT+TGG	-	chr5.2:59261454-59261473	None:intergenic	35.0%
	GAAACGAGACATTAAGTCAT+CGG	-	chr5.2:59262678-59262697	None:intergenic	35.0%
	GAGAATGATCAAGTTACTCA+AGG	-	chr5.2:59262433-59262452	None:intergenic	35.0%
	GATAATGCATGTTTGGTATG+TGG	-	chr5.2:59261447-59261466	None:intergenic	35.0%
	GCTTATTGTGAAACGTGATT+TGG	+	chr5.2:59261989-59262008	MS.gene44877:intron	35.0%
	GTGTCAAAAGCAACAGAAAA+GGG	-	chr5.2:59261881-59261900	None:intergenic	35.0%
	TGTCAAAAGCAACAGAAAAG+GGG	-	chr5.2:59261880-59261899	None:intergenic	35.0%
!	AAACCGACTATGGTGTATTT+TGG	+	chr5.2:59261727-59261746	MS.gene44877:CDS	35.0%
!	AACTGCATTTCACGATCTTT+TGG	+	chr5.2:59261583-59261602	MS.gene44877:CDS	35.0%
!	ACTGCATTTCACGATCTTTT+GGG	+	chr5.2:59261584-59261603	MS.gene44877:CDS	35.0%
!	CATTATCCCTTTTCCTTTCT+TGG	+	chr5.2:59261460-59261479	MS.gene44877:CDS	35.0%
	AGACATTAAGTCATCGGAAG+GGG	-	chr5.2:59262672-59262691	None:intergenic	40.0%
	ATATATAGGTTCCTCTACCC+AGG	+	chr5.2:59262533-59262552	MS.gene44877:intron	40.0%
	GAAACCTCCATATTAGACCT+GGG	-	chr5.2:59262553-59262572	None:intergenic	40.0%
	GAGACATTAAGTCATCGGAA+GGG	-	chr5.2:59262673-59262692	None:intergenic	40.0%
	GAGATGGAACCAAAGGAAAT+AGG	-	chr5.2:59261300-59261319	None:intergenic	40.0%
	GAGGAGGAGAAAAGCAAAAA+TGG	+	chr5.2:59262888-59262907	MS.gene44877:CDS	40.0%
	GGTGTCAAAAGCAACAGAAA+AGG	-	chr5.2:59261882-59261901	None:intergenic	40.0%
	GGTTCGATAATTACCCGAAA+GGG	+	chr5.2:59261549-59261568	MS.gene44877:CDS	40.0%
	GGTTTATTTGAAAGCGGCAT+TGG	+	chr5.2:59262949-59262968	MS.gene44877:CDS	40.0%
	TGAAACCTCCATATTAGACC+TGG	-	chr5.2:59262554-59262573	None:intergenic	40.0%
	TGTGAGCCAAGAAAGGAAAA+GGG	-	chr5.2:59261469-59261488	None:intergenic	40.0%
	TTGTGAGCCAAGAAAGGAAA+AGG	-	chr5.2:59261470-59261489	None:intergenic	40.0%
	TTTCAGTGAAGCGGATCAAT+TGG	+	chr5.2:59262928-59262947	MS.gene44877:CDS	40.0%
!	AGGATGTGTTCGAGGTTTTT+AGG	-	chr5.2:59261521-59261540	None:intergenic	40.0%
!	CTTTCGGGTAATTATCGAAC+CGG	-	chr5.2:59261550-59261569	None:intergenic	40.0%
!	GGATGTGTTCGAGGTTTTTA+GGG	-	chr5.2:59261520-59261539	None:intergenic	40.0%
!	TCGGTTCCGGTTTTTGAATA+GGG	-	chr5.2:59261378-59261397	None:intergenic	40.0%
!	TTATTTGAAAGCGGCATTGG+CGG	+	chr5.2:59262952-59262971	MS.gene44877:CDS	40.0%
!	TTGTGAAACGTGATTTGGGT+AGG	+	chr5.2:59261994-59262013	MS.gene44877:intron	40.0%
!	TTTCGGGTAATTATCGAACC+GGG	-	chr5.2:59261549-59261568	None:intergenic	40.0%
!	TTTTCTCCTCAACTTTCGGA+TGG	-	chr5.2:59262832-59262851	None:intergenic	40.0%
!!	ACACCATAGTCGGTTTTTGA+TGG	-	chr5.2:59261723-59261742	None:intergenic	40.0%
!!	ATAGTCGGTTTTTGATGGAC+CGG	-	chr5.2:59261718-59261737	None:intergenic	40.0%
	AAACTTCATCTTGGCCGGAA+GGG	+	chr5.2:59262760-59262779	MS.gene44877:CDS	45.0%
	AAGAGTTACTGACCTGTGCA+TGG	-	chr5.2:59261918-59261937	None:intergenic	45.0%
	AATTATCGAACCGGGTTCGA+AGG	-	chr5.2:59261541-59261560	None:intergenic	45.0%
	ACCAAAGGAAATAGGCAGAG+AGG	-	chr5.2:59261292-59261311	None:intergenic	45.0%
	ACTGTTCTTGAGGAAACTCG+AGG	+	chr5.2:59262869-59262888	MS.gene44877:CDS	45.0%
	AGACCCTGAAACTCTTTCAC+CGG	+	chr5.2:59261841-59261860	MS.gene44877:CDS	45.0%
	CATGAACCATCCGAAAGTTG+AGG	+	chr5.2:59262823-59262842	MS.gene44877:CDS	45.0%
	CCAAAGGAAATAGGCAGAGA+GGG	-	chr5.2:59261291-59261310	None:intergenic	45.0%
	CGAGACATTAAGTCATCGGA+AGG	-	chr5.2:59262674-59262693	None:intergenic	45.0%
	CGGTTCGATAATTACCCGAA+AGG	+	chr5.2:59261548-59261567	MS.gene44877:CDS	45.0%
	CTTTCTTGGCTCACAAGCAA+GGG	+	chr5.2:59261474-59261493	MS.gene44877:CDS	45.0%
	GAAACTAGCTATGGCTCGAT+GGG	+	chr5.2:59261688-59261707	MS.gene44877:CDS	45.0%
	GGAGTTAACCACTGTTCTTG+AGG	+	chr5.2:59262859-59262878	MS.gene44877:CDS	45.0%
	GGTCCATCAAAAACCGACTA+TGG	+	chr5.2:59261717-59261736	MS.gene44877:CDS	45.0%
	GGTTTCCTTGCGATCTGAAA+TGG	-	chr5.2:59262651-59262670	None:intergenic	45.0%
	TAAACTTCATCTTGGCCGGA+AGG	+	chr5.2:59262759-59262778	MS.gene44877:CDS	45.0%
	TAGGGTAAGGAGAAGAGTGA+GGG	-	chr5.2:59261261-59261280	None:intergenic	45.0%
	TCCAATCATGAACTCGGTTC+CGG	-	chr5.2:59261391-59261410	None:intergenic	45.0%
	TCCATATTAGACCTGGGTAG+AGG	-	chr5.2:59262547-59262566	None:intergenic	45.0%
	TCCGCTTCACTGAAATCAAG+CGG	-	chr5.2:59262923-59262942	None:intergenic	45.0%
	TCCGCTTGATTTCAGTGAAG+CGG	+	chr5.2:59262919-59262938	MS.gene44877:CDS	45.0%
	TCCTCTACCCAGGTCTAATA+TGG	+	chr5.2:59262543-59262562	MS.gene44877:CDS	45.0%
	TCGAGTTTCCTCAAGAACAG+TGG	-	chr5.2:59262870-59262889	None:intergenic	45.0%
	TCTACCCAGGTCTAATATGG+AGG	+	chr5.2:59262546-59262565	MS.gene44877:CDS	45.0%
	TGAAACTAGCTATGGCTCGA+TGG	+	chr5.2:59261687-59261706	MS.gene44877:CDS	45.0%
	TGAAATGCAGTTTGCCACGT+AGG	-	chr5.2:59261575-59261594	None:intergenic	45.0%
	TTAGGGTAAGGAGAAGAGTG+AGG	-	chr5.2:59261262-59261281	None:intergenic	45.0%
	TTCCGGCCCTATTCAAAAAC+CGG	+	chr5.2:59261369-59261388	MS.gene44877:CDS	45.0%
!	AACTGATGATGTGTCCCTTC+CGG	-	chr5.2:59262777-59262796	None:intergenic	45.0%
!	CTCGGTTCCGGTTTTTGAAT+AGG	-	chr5.2:59261379-59261398	None:intergenic	45.0%
!	GCAACAGAAAAGGGGTTTTC+AGG	-	chr5.2:59261872-59261891	None:intergenic	45.0%
!	TGTAGCACTGTTGCGTTGAT+TGG	-	chr5.2:59262728-59262747	None:intergenic	45.0%
!	TTCCGGTTTTTGAATAGGGC+CGG	-	chr5.2:59261374-59261393	None:intergenic	45.0%
!	TTTCACGATCTTTTGGGCCA+AGG	+	chr5.2:59261590-59261609	MS.gene44877:CDS	45.0%
!!	CAAGGCATTTTCAACAGCGA+CGG	+	chr5.2:59261608-59261627	MS.gene44877:CDS	45.0%
!!	TGTGTTCGAGGTTTTTAGGG+TGG	-	chr5.2:59261517-59261536	None:intergenic	45.0%
!!!	GTTTTTAGGGTGGCATGTGA+CGG	-	chr5.2:59261507-59261526	None:intergenic	45.0%
	AACGCCGGTGAAAGAGTTTC+AGG	-	chr5.2:59261848-59261867	None:intergenic	50.0%
	AAGGAAATAGGCAGAGAGGG+AGG	-	chr5.2:59261288-59261307	None:intergenic	50.0%
	ACCGGAACCGAGTTCATGAT+TGG	+	chr5.2:59261387-59261406	MS.gene44877:CDS	50.0%
	ACGCCGGTGAAAGAGTTTCA+GGG	-	chr5.2:59261847-59261866	None:intergenic	50.0%
	ACTGCCTGCTTGATATCCTG+CGG	-	chr5.2:59263004-59263023	None:intergenic	50.0%
	AGTTACTGACCTGTGCATGG+TGG	-	chr5.2:59261915-59261934	None:intergenic	50.0%
	ATGTTCGTGCCGTGAATTCG+AGG	-	chr5.2:59261667-59261686	None:intergenic	50.0%
	CAACGCCATTTCAGATCGCA+AGG	+	chr5.2:59262643-59262662	MS.gene44877:CDS	50.0%
	CATCATCAGTTGCTCTCAGC+TGG	+	chr5.2:59262786-59262805	MS.gene44877:CDS	50.0%
	CCCTCTCTGCCTATTTCCTT+TGG	+	chr5.2:59261288-59261307	MS.gene44877:CDS	50.0%
	CCGAGTTCATGATTGGATCG+CGG	+	chr5.2:59261394-59261413	MS.gene44877:CDS	50.0%
	CCGCGATCCAATCATGAACT+CGG	-	chr5.2:59261397-59261416	None:intergenic	50.0%
	CCTTGCTTGTGAGCCAAGAA+AGG	-	chr5.2:59261476-59261495	None:intergenic	50.0%
	CCTTTCTTGGCTCACAAGCA+AGG	+	chr5.2:59261473-59261492	MS.gene44877:CDS	50.0%
	CGATGGAACAGTTGTTCCAG+CGG	+	chr5.2:59263042-59263061	MS.gene44877:CDS	50.0%
	CGCTGGAACAACTGTTCCAT+CGG	-	chr5.2:59263044-59263063	None:intergenic	50.0%
	CGTCGCTGTTGAAAATGCCT+TGG	-	chr5.2:59261610-59261629	None:intergenic	50.0%
	GAAAATCTCCGAGCAACTGG+TGG	+	chr5.2:59261416-59261435	MS.gene44877:CDS	50.0%
	GCTGGAACAACTGTTCCATC+GGG	-	chr5.2:59263043-59263062	None:intergenic	50.0%
	GTTCTTGAGGAAACTCGAGG+AGG	+	chr5.2:59262872-59262891	MS.gene44877:CDS	50.0%
	TCGAACACATCCTTCGAACC+CGG	+	chr5.2:59261528-59261547	MS.gene44877:CDS	50.0%
	TGTTCGTGCCGTGAATTCGA+GGG	-	chr5.2:59261666-59261685	None:intergenic	50.0%
!	CCAAAAGCATGGCCCTTCAT+CGG	+	chr5.2:59261341-59261360	MS.gene44877:CDS	50.0%
!	CCATGCTTTTGGACCGGTTA+GGG	-	chr5.2:59261333-59261352	None:intergenic	50.0%
!	CTTTTGGACCGGTTAGGGTT+CGG	-	chr5.2:59261328-59261347	None:intergenic	50.0%
!	GGTTTTTGAATAGGGCCGGA+AGG	-	chr5.2:59261370-59261389	None:intergenic	50.0%
!	GTGGACGATGTTTTTCCCGA+TGG	+	chr5.2:59263025-59263044	MS.gene44877:CDS	50.0%
!	TTGATATCCTGCGGCACTGA+CGG	-	chr5.2:59262995-59263014	None:intergenic	50.0%
!	TTGCTCTCAGCTGGTTCTTC+TGG	+	chr5.2:59262795-59262814	MS.gene44877:CDS	50.0%
!	TTTTGGACCGGTTAGGGTTC+GGG	-	chr5.2:59261327-59261346	None:intergenic	50.0%
!!!	AAGGGGTTTTCAGGTAACGC+CGG	-	chr5.2:59261863-59261882	None:intergenic	50.0%
	AACCGCTGCCCTCGAATTCA+CGG	+	chr5.2:59261655-59261674	MS.gene44877:CDS	55.0%
	AGAGGGAGGCTGTTAGGGTA+AGG	-	chr5.2:59261274-59261293	None:intergenic	55.0%
	AGTGCCGCAGGATATCAAGC+AGG	+	chr5.2:59262997-59263016	MS.gene44877:CDS	55.0%
	ATTACCCGAAAGGGCCTACG+TGG	+	chr5.2:59261558-59261577	MS.gene44877:CDS	55.0%
	CCCTAACCGGTCCAAAAGCA+TGG	+	chr5.2:59261330-59261349	MS.gene44877:CDS	55.0%
	CTATGGCTCGATGGGTGAAC+CGG	+	chr5.2:59261696-59261715	MS.gene44877:CDS	55.0%
	GCGGAAAATCTCCGAGCAAC+TGG	+	chr5.2:59261413-59261432	MS.gene44877:CDS	55.0%
	GGATATCAAGCAGGCAGTCG+TGG	+	chr5.2:59263006-59263025	MS.gene44877:CDS	55.0%
	GGGTTCGAAGGATGTGTTCG+AGG	-	chr5.2:59261529-59261548	None:intergenic	55.0%
	GTTTGCCACGTAGGCCCTTT+CGG	-	chr5.2:59261566-59261585	None:intergenic	55.0%
	TCAACAGCGACGGTGAAACG+TGG	+	chr5.2:59261618-59261637	MS.gene44877:CDS	55.0%
	TGGCTGAACCACCAGTTGCT+CGG	-	chr5.2:59261427-59261446	None:intergenic	55.0%
	TTTGCCACGTAGGCCCTTTC+GGG	-	chr5.2:59261565-59261584	None:intergenic	55.0%
	TTTGTATCCGTCAGTGCCGC+AGG	+	chr5.2:59262985-59263004	MS.gene44877:CDS	55.0%
!	CCGATGAAGGGCCATGCTTT+TGG	-	chr5.2:59261344-59261363	None:intergenic	55.0%
!	GAAGGGCCATGCTTTTGGAC+CGG	-	chr5.2:59261339-59261358	None:intergenic	55.0%
!	GCCATGCTTTTGGACCGGTT+AGG	-	chr5.2:59261334-59261353	None:intergenic	55.0%
!	TCTCAGCTGGTTCTTCTGGC+TGG	+	chr5.2:59262799-59262818	MS.gene44877:CDS	55.0%
	AGGCAGAGAGGGAGGCTGTT+AGG	-	chr5.2:59261280-59261299	None:intergenic	60.0%
	CCACTGAAGCCACCATGCAC+AGG	+	chr5.2:59261903-59261922	MS.gene44877:CDS	60.0%
	CCTGTGCATGGTGGCTTCAG+TGG	-	chr5.2:59261906-59261925	None:intergenic	60.0%
	GCCCTTCATCGGTAGCCTTC+CGG	+	chr5.2:59261352-59261371	MS.gene44877:CDS	60.0%
	GCCGGAAGGCTACCGATGAA+GGG	-	chr5.2:59261356-59261375	None:intergenic	60.0%
	GGCAGAGAGGGAGGCTGTTA+GGG	-	chr5.2:59261279-59261298	None:intergenic	60.0%
	GGGCGAGGAGATGGAACCAA+AGG	-	chr5.2:59261307-59261326	None:intergenic	60.0%
	GTGCATGGTGGCTTCAGTGG+CGG	-	chr5.2:59261903-59261922	None:intergenic	60.0%
	TGCCGTGAATTCGAGGGCAG+CGG	-	chr5.2:59261660-59261679	None:intergenic	60.0%
	TTAGGGTTCGGGCGAGGAGA+TGG	-	chr5.2:59261316-59261335	None:intergenic	60.0%
	CTCCTCGCCCGAACCCTAAC+CGG	+	chr5.2:59261317-59261336	MS.gene44877:CDS	65.0%
	GGCCGGAAGGCTACCGATGA+AGG	-	chr5.2:59261357-59261376	None:intergenic	65.0%
	GACCGGTTAGGGTTCGGGCG+AGG	-	chr5.2:59261322-59261341	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	59261251	59263064	59261251	ID=MS.gene44877
chr5.2	mRNA	59261251	59263064	59261251	ID=MS.gene44877.t1;Parent=MS.gene44877
chr5.2	exon	59261251	59261924	59261251	ID=MS.gene44877.t1.exon1;Parent=MS.gene44877.t1
chr5.2	CDS	59261251	59261924	59261251	ID=cds.MS.gene44877.t1;Parent=MS.gene44877.t1
chr5.2	exon	59262541	59263064	59262541	ID=MS.gene44877.t1.exon2;Parent=MS.gene44877.t1
chr5.2	CDS	59262541	59263064	59262541	ID=cds.MS.gene44877.t1;Parent=MS.gene44877.t1

Gene Sequence

>MS.gene44877

ATGGAGACCCTCACTCTTCTCCTTACCCTAACAGCCTCCCTCTCTGCCTATTTCCTTTGGTTCCATCTCCTCGCCCGAACCCTAACCGGTCCAAAAGCATGGCCCTTCATCGGTAGCCTTCCGGCCCTATTCAAAAACCGGAACCGAGTTCATGATTGGATCGCGGAAAATCTCCGAGCAACTGGTGGTTCAGCCACATACCAAACATGCATTATCCCTTTTCCTTTCTTGGCTCACAAGCAAGGGTTCTACACCGTCACATGCCACCCTAAAAACCTCGAACACATCCTTCGAACCCGGTTCGATAATTACCCGAAAGGGCCTACGTGGCAAACTGCATTTCACGATCTTTTGGGCCAAGGCATTTTCAACAGCGACGGTGAAACGTGGATAATGCAACGTAAAACCGCTGCCCTCGAATTCACGGCACGAACATTGAAACTAGCTATGGCTCGATGGGTGAACCGGTCCATCAAAAACCGACTATGGTGTATTTTGGATAAATCGGTTAAAGATAATGTCTATGTGGATTTACAAGATCTTTTGTTAAGATTAACATTTGATAATATTTGTGGACTTACGTTAGGTAAAGACCCTGAAACTCTTTCACCGGCGTTACCTGAAAACCCCTTTTCTGTTGCTTTTGACACCGCCACTGAAGCCACCATGCACAGGTCAGTAACTCTTACTTATAGTTGTAGACATATGGTTTTGAATCTTAATTAAGTTCATGTTTGAGCTTATTGTGAAACGTGATTTGGGTAGGATTAATTAATGAGTCGCTTGTCAAAAAAAATCATCAGTCACATGTCATAATTAACTTAATTTGCTTTCTGTTTAAAACTAATAAAAATCATTTTGCTTTATTTTTCCCTTAAAAAGAAAAACCATTTTGCTTTATTTTTAAAATTTAATTCATATACGTCATCAATGTGAATAGTTAACCAGCTAATCACTAAAATGTTTAACATTTAGATTAATTATTTCTAAAGTCACACATGATTCATTGTGATTTTCCTGAACAAAACTTGTCAAAAATTAAATTTTTTCTTTTATATATAATGTGAATTTCGTTTTGATAATATAATATAATAAGAGAGGTCATGTTATTCCAAGCATATATTTTAGTTGAGTTATTCCGTTTAATAATTGACAGAATTTTGTCAACTCAAACTTGCTCCTTGAGTAACTTGATCATTCTCATTAATAAATAGTGTTATTTCGATACATGTTGAGATTATAATTTAGTCATCTATCATTCTCACAAAAATAATTGCTCAACATATATAGGTTCCTCTACCCAGGTCTAATATGGAGGTTTCAAAAACTTTTCGGCATAGGATCCGAAAAAATGTTAAAACAAAGTCTCCAAATTGTAGAAACATACATGAACAACGCCATTTCAGATCGCAAGGAAACCCCTTCCGATGACTTAATGTCTCGTTTCATGAAGAAACGAGACATAGATGGAAAACCAATCAACGCAACAGTGCTACAACACATAATCTTAAACTTCATCTTGGCCGGAAGGGACACATCATCAGTTGCTCTCAGCTGGTTCTTCTGGCTGGTCATGAACCATCCGAAAGTTGAGGAGAAAATAATCAAGGAGTTAACCACTGTTCTTGAGGAAACTCGAGGAGGAGAAAAGCAAAAATGGACAGAGGATCCGCTTGATTTCAGTGAAGCGGATCAATTGGTTTATTTGAAAGCGGCATTGGCGGAAACACTGCGTTTGTATCCGTCAGTGCCGCAGGATATCAAGCAGGCAGTCGTGGACGATGTTTTTCCCGATGGAACAGTTGTTCCAGCGG

Protein sequence

>MS.gene44877.t1

METLTLLLTLTASLSAYFLWFHLLARTLTGPKAWPFIGSLPALFKNRNRVHDWIAENLRATGGSATYQTCIIPFPFLAHKQGFYTVTCHPKNLEHILRTRFDNYPKGPTWQTAFHDLLGQGIFNSDGETWIMQRKTAALEFTARTLKLAMARWVNRSIKNRLWCILDKSVKDNVYVDLQDLLLRLTFDNICGLTLGKDPETLSPALPENPFSVAFDTATEATMHRFLYPGLIWRFQKLFGIGSEKMLKQSLQIVETYMNNAISDRKETPSDDLMSRFMKKRDIDGKPINATVLQHIILNFILAGRDTSSVALSWFFWLVMNHPKVEEKIIKELTTVLEETRGGEKQKWTEDPLDFSEADQLVYLKAALAETLRLYPSVPQDIKQAVVDDVFPDGTVVPA