Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044581.t1 | XP_003600054.1 | 96 | 251 | 10 | 0 | 142 | 392 | 1 | 251 | 3.60E-134 | 488.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044581.t1 | Q5M759 | 80.4 | 250 | 49 | 0 | 142 | 391 | 1 | 250 | 7.6e-117 | 422.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044581.t1 | G7IXK4 | 96.0 | 251 | 10 | 0 | 142 | 392 | 1 | 251 | 2.6e-134 | 488.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene77803 | MS.gene044581 | PPI |
| MS.gene028008 | MS.gene044581 | PPI |
| MS.gene94059 | MS.gene044581 | PPI |
| MS.gene016363 | MS.gene044581 | PPI |
| MS.gene59808 | MS.gene044581 | PPI |
| MS.gene72906 | MS.gene044581 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044581.t1 | MTR_3g051210 | 96.016 | 251 | 10 | 0 | 142 | 392 | 1 | 251 | 1.20e-178 | 499 |
| MS.gene044581.t1 | MTR_7g033650 | 94.821 | 251 | 13 | 0 | 142 | 392 | 1 | 251 | 3.07e-175 | 491 |
| MS.gene044581.t1 | MTR_7g033680 | 90.400 | 125 | 7 | 3 | 1 | 125 | 1 | 120 | 1.18e-72 | 225 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044581.t1 | AT4G27040 | 80.400 | 250 | 49 | 0 | 142 | 391 | 1 | 250 | 8.28e-152 | 431 |
| MS.gene044581.t1 | AT4G27040 | 80.400 | 250 | 49 | 0 | 142 | 391 | 1 | 250 | 8.28e-152 | 431 |
| MS.gene044581.t1 | AT4G27040 | 80.400 | 250 | 49 | 0 | 142 | 391 | 1 | 250 | 8.28e-152 | 431 |
| MS.gene044581.t1 | AT4G27040 | 80.400 | 250 | 49 | 0 | 142 | 391 | 1 | 250 | 8.28e-152 | 431 |
| MS.gene044581.t1 | AT4G27040 | 80.400 | 250 | 49 | 0 | 142 | 391 | 1 | 250 | 8.28e-152 | 431 |
| MS.gene044581.t1 | AT4G27040 | 80.400 | 250 | 49 | 0 | 142 | 391 | 1 | 250 | 8.28e-152 | 431 |
| MS.gene044581.t1 | AT5G40500 | 55.556 | 126 | 43 | 5 | 1 | 123 | 1 | 116 | 8.47e-37 | 131 |
| MS.gene044581.t1 | AT5G40500 | 56.911 | 123 | 37 | 4 | 1 | 123 | 1 | 107 | 9.58e-37 | 131 |
Find 94 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCCCTCTTGGCTCCGATTT+TGG | 0.079856 | 7.3:+65885154 | MS.gene044581:CDS |
| CCAGATTACACGAGAGTTAA+AGG | 0.174737 | 7.3:+65881886 | MS.gene044581:CDS |
| AAGAGATCAATATAAGAAAT+TGG | 0.210738 | 7.3:+65881987 | MS.gene044581:CDS |
| TGCGCTTAATTCTCAGCTTT+TGG | 0.214875 | 7.3:+65884926 | MS.gene044581:CDS |
| AGATCCATTGGCCTCGAATA+AGG | 0.248372 | 7.3:+65882238 | MS.gene044581:CDS |
| GTAATCTCAATAACTTTATA+TGG | 0.248815 | 7.3:-65872880 | None:intergenic |
| AATTCCCGTCAAAGACAAAA+TGG | 0.251935 | 7.3:-65884052 | None:intergenic |
| AGGCGACTATCTTGGATTTC+GGG | 0.267213 | 7.3:+65883837 | MS.gene044581:CDS |
| AAGGCGACTATCTTGGATTT+CGG | 0.297940 | 7.3:+65883836 | MS.gene044581:CDS |
| CCTTTAACTCTCGTGTAATC+TGG | 0.323351 | 7.3:-65881886 | None:intergenic |
| ATGAGAAGGAGAGCTGGAAT+TGG | 0.347741 | 7.3:+65881940 | MS.gene044581:CDS |
| CGTATTTCCCTTTGCGAAGC+TGG | 0.348117 | 7.3:-65877213 | None:intergenic |
| CACACGAGCTACCGAATACT+TGG | 0.355430 | 7.3:-65885200 | None:intergenic |
| TCAAATTGTGGACATTTGTT+TGG | 0.356074 | 7.3:+65882400 | MS.gene044581:CDS |
| TTGCACCTTCCCCAAGTATT+CGG | 0.381447 | 7.3:+65885189 | MS.gene044581:CDS |
| TGGGCAGAGCTATTAGGAAT+TGG | 0.401013 | 7.3:+65882266 | MS.gene044581:CDS |
| AGAGACATCTTCGCCAAAAT+CGG | 0.402214 | 7.3:-65885167 | None:intergenic |
| TTTCATAGACTTTGTGCAAA+AGG | 0.404737 | 7.3:+65885103 | MS.gene044581:CDS |
| TGTGCAGACTATGTTGTTTG+AGG | 0.408379 | 7.3:+65872798 | MS.gene044581:intron |
| GATCCATTGGCCTCGAATAA+GGG | 0.429732 | 7.3:+65882239 | MS.gene044581:CDS |
| ACTGGGGCTTTCCAGAAAAC+AGG | 0.437782 | 7.3:+65881847 | MS.gene044581:intron |
| AGAAGGAGAGCTGGAATTGG+AGG | 0.444875 | 7.3:+65881943 | MS.gene044581:CDS |
| GGAGTTGACAGAGTTCTTGT+AGG | 0.447771 | 7.3:-65882454 | None:intergenic |
| AGAGATCAATATAAGAAATT+GGG | 0.448974 | 7.3:+65881988 | MS.gene044581:CDS |
| GGTGATTTCTATTACGAACT+TGG | 0.449692 | 7.3:+65882287 | MS.gene044581:CDS |
| AAGTAGAAAGGCGACTATCT+TGG | 0.451104 | 7.3:+65883829 | MS.gene044581:CDS |
| TGGTCTTTGTTCAGCTCAGT+TGG | 0.452013 | 7.3:-65883005 | None:intergenic |
| AAAGGAATTGATATTCAAAT+CGG | 0.454636 | 7.3:+65881913 | MS.gene044581:CDS |
| TCACAAATATTTCAGGTTCT+CGG | 0.456872 | 7.3:+65882930 | MS.gene044581:intron |
| ATTCCCGTCAAAGACAAAAT+GGG | 0.463136 | 7.3:-65884051 | None:intergenic |
| ACATCTTAGGATCTCGCTTC+AGG | 0.466035 | 7.3:-65885226 | None:intergenic |
| ACAACGAACGCCAAGAGACT+TGG | 0.469904 | 7.3:-65872911 | None:intergenic |
| GGAGAGAGTGTCACAATTGA+AGG | 0.473888 | 7.3:+65877154 | MS.gene044581:CDS |
| TAATGTATCAAGTGCATCAA+TGG | 0.476110 | 7.3:-65883865 | None:intergenic |
| ATTGATGATGGCCACAAAGA+TGG | 0.476925 | 7.3:+65883997 | MS.gene044581:CDS |
| TATTTCAGGTTCTCGGTAGC+GGG | 0.477130 | 7.3:+65882937 | MS.gene044581:intron |
| CTATTTCAGCTGTAACGCTT+CGG | 0.481394 | 7.3:+65877188 | MS.gene044581:CDS |
| ATAAAATTTATAGGCGGTTG+AGG | 0.481678 | 7.3:+65885072 | MS.gene044581:intron |
| TTGTGCAAAAGGTGCCATCT+TGG | 0.482010 | 7.3:+65885114 | MS.gene044581:CDS |
| ATATTTCAGGTTCTCGGTAG+CGG | 0.490149 | 7.3:+65882936 | MS.gene044581:intron |
| CATGAAATGTGTGCTAAAGT+TGG | 0.496026 | 7.3:+65882212 | MS.gene044581:CDS |
| ACTCCTCCACCCAAGTCTCT+TGG | 0.496625 | 7.3:+65872901 | MS.gene044581:CDS |
| TCTCAAAATCCTCGCCCTCT+TGG | 0.500673 | 7.3:+65885142 | MS.gene044581:CDS |
| TTGAAGCTGAGAACGAAAAG+TGG | 0.513721 | 7.3:-65882058 | None:intergenic |
| TAATCTCAATAACTTTATAT+GGG | 0.517341 | 7.3:-65872879 | None:intergenic |
| TTCGGTACCAGCTTCGCAAA+GGG | 0.521585 | 7.3:+65877206 | MS.gene044581:CDS |
| GGGTTTGAAGTTATTTCTGT+TGG | 0.525591 | 7.3:+65882957 | MS.gene044581:CDS |
| GATCAGAACTGATTTGATGA+AGG | 0.529766 | 7.3:+65882026 | MS.gene044581:CDS |
| AAGACCTATGTTCATCATGC+TGG | 0.531340 | 7.3:-65872669 | None:intergenic |
| CTTGGTTTACTTCAGGGTCA+AGG | 0.537128 | 7.3:+65883789 | MS.gene044581:intron |
| TGAAACTGTGATCTGAATGC+AGG | 0.546795 | 7.3:-65882191 | None:intergenic |
| AAATCGGAGCCAAGAGGGCG+AGG | 0.548446 | 7.3:-65885151 | None:intergenic |
| TTGGCTACTAGACCCCTCAA+TGG | 0.554537 | 7.3:+65882419 | MS.gene044581:CDS |
| ACACGAGCTACCGAATACTT+GGG | 0.562572 | 7.3:-65885199 | None:intergenic |
| GAAGGAGAGCTGGAATTGGA+GGG | 0.569907 | 7.3:+65881944 | MS.gene044581:CDS |
| AAACCCTTATTCGAGGCCAA+TGG | 0.570088 | 7.3:-65882242 | None:intergenic |
| ACGAGAGTTAAAGGAAGGAA+AGG | 0.570101 | 7.3:+65881895 | MS.gene044581:CDS |
| CTTCGGTACCAGCTTCGCAA+AGG | 0.571486 | 7.3:+65877205 | MS.gene044581:CDS |
| TCTCAATAACTTTATATGGG+TGG | 0.572214 | 7.3:-65872876 | None:intergenic |
| GCTACTAGACCCCTCAATGG+TGG | 0.573069 | 7.3:+65882422 | MS.gene044581:CDS |
| CAACGAACGCCAAGAGACTT+GGG | 0.574152 | 7.3:-65872910 | None:intergenic |
| AATACGAACAAAGCGAAAAG+AGG | 0.584810 | 7.3:+65877230 | MS.gene044581:CDS |
| GTTGATCAATCCACCATTGA+GGG | 0.587064 | 7.3:-65882432 | None:intergenic |
| TTCCCGTCAAAGACAAAATG+GGG | 0.588402 | 7.3:-65884050 | None:intergenic |
| GGTTGATCAATCCACCATTG+AGG | 0.588512 | 7.3:-65882433 | None:intergenic |
| TCGTGCTATCAGTAAGCTGA+AGG | 0.589233 | 7.3:+65882529 | MS.gene044581:CDS |
| AAGATGGAAAACGACGGTAC+TGG | 0.589640 | 7.3:+65884013 | MS.gene044581:CDS |
| TAAAGTTGGAGTAGATCCAT+TGG | 0.592159 | 7.3:+65882226 | MS.gene044581:CDS |
| TCATCCAGCATGATGAACAT+AGG | 0.592518 | 7.3:+65872665 | MS.gene044581:CDS |
| AAAGATTTGGAGAATGGCAA+TGG | 0.592847 | 7.3:+65872640 | None:intergenic |
| TTCATCATACAAGAAGAAGA+AGG | 0.597762 | 7.3:+65872694 | MS.gene044581:CDS |
| GGACTTGCAATGATTGATGA+TGG | 0.603574 | 7.3:+65883985 | MS.gene044581:CDS |
| ACGCCAAGAGACTTGGGTGG+AGG | 0.606321 | 7.3:-65872904 | None:intergenic |
| TGACTGTTGATGAAGTAGAA+AGG | 0.610720 | 7.3:+65883817 | MS.gene044581:CDS |
| CGCCAAAATCGGAGCCAAGA+GGG | 0.612643 | 7.3:-65885156 | None:intergenic |
| ATTACACGAGAGTTAAAGGA+AGG | 0.617515 | 7.3:+65881890 | MS.gene044581:CDS |
| TATCAAGTGCATCAATGGCA+CGG | 0.623758 | 7.3:-65883860 | None:intergenic |
| TCAGCTCAGTTGGAACCGAA+CGG | 0.627094 | 7.3:-65882995 | None:intergenic |
| AGTATTCGGTAGCTCGTGTG+TGG | 0.629137 | 7.3:+65885203 | MS.gene044581:CDS |
| GAAACTGTGATCTGAATGCA+GGG | 0.646444 | 7.3:-65882190 | None:intergenic |
| CTAGACAGGAGTTCAAATTG+TGG | 0.654031 | 7.3:+65882388 | MS.gene044581:intron |
| AGCTACCGAATACTTGGGGA+AGG | 0.661241 | 7.3:-65885194 | None:intergenic |
| GCTGAGAACGAAAAGTGGAA+AGG | 0.668139 | 7.3:-65882053 | None:intergenic |
| TTAAAGCAGAACTTGCAATG+TGG | 0.668299 | 7.3:+65877133 | MS.gene044581:intron |
| AGAGACAGAGACAGAGAACG+AGG | 0.670989 | 7.3:+65872838 | MS.gene044581:CDS |
| CACGAGCTACCGAATACTTG+GGG | 0.671377 | 7.3:-65885198 | None:intergenic |
| TTGATCAATCCACCATTGAG+GGG | 0.680680 | 7.3:-65882431 | None:intergenic |
| TGACCGTGGAGTTGTTTCTG+AGG | 0.693759 | 7.3:+65882496 | MS.gene044581:CDS |
| TCGCCAAAATCGGAGCCAAG+AGG | 0.695160 | 7.3:-65885157 | None:intergenic |
| TCCCGTCAAAGACAAAATGG+GGG | 0.701921 | 7.3:-65884049 | None:intergenic |
| CGAACGCCAAGAGACTTGGG+TGG | 0.702523 | 7.3:-65872907 | None:intergenic |
| CATCCTCAGAAACAACTCCA+CGG | 0.709527 | 7.3:-65882499 | None:intergenic |
| GCCACAAAGATGGAAAACGA+CGG | 0.745304 | 7.3:+65884007 | MS.gene044581:CDS |
| TCAGCTTACTGATAGCACGA+AGG | 0.745994 | 7.3:-65882526 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 65872653 | 65885243 | 65872653 | ID=MS.gene044581 |
| chr7.3 | mRNA | 65872653 | 65885243 | 65872653 | ID=MS.gene044581.t1;Parent=MS.gene044581 |
| chr7.3 | exon | 65872653 | 65872715 | 65872653 | ID=MS.gene044581.t1.exon1;Parent=MS.gene044581.t1 |
| chr7.3 | CDS | 65872653 | 65872715 | 65872653 | ID=cds.MS.gene044581.t1;Parent=MS.gene044581.t1 |
| chr7.3 | exon | 65872805 | 65872942 | 65872805 | ID=MS.gene044581.t1.exon2;Parent=MS.gene044581.t1 |
| chr7.3 | CDS | 65872805 | 65872942 | 65872805 | ID=cds.MS.gene044581.t1;Parent=MS.gene044581.t1 |
| chr7.3 | exon | 65877139 | 65877282 | 65877139 | ID=MS.gene044581.t1.exon3;Parent=MS.gene044581.t1 |
| chr7.3 | CDS | 65877139 | 65877282 | 65877139 | ID=cds.MS.gene044581.t1;Parent=MS.gene044581.t1 |
| chr7.3 | exon | 65881862 | 65882101 | 65881862 | ID=MS.gene044581.t1.exon4;Parent=MS.gene044581.t1 |
| chr7.3 | CDS | 65881862 | 65882101 | 65881862 | ID=cds.MS.gene044581.t1;Parent=MS.gene044581.t1 |
| chr7.3 | exon | 65882173 | 65882308 | 65882173 | ID=MS.gene044581.t1.exon5;Parent=MS.gene044581.t1 |
| chr7.3 | CDS | 65882173 | 65882308 | 65882173 | ID=cds.MS.gene044581.t1;Parent=MS.gene044581.t1 |
| chr7.3 | exon | 65882396 | 65882550 | 65882396 | ID=MS.gene044581.t1.exon6;Parent=MS.gene044581.t1 |
| chr7.3 | CDS | 65882396 | 65882550 | 65882396 | ID=cds.MS.gene044581.t1;Parent=MS.gene044581.t1 |
| chr7.3 | exon | 65882945 | 65883052 | 65882945 | ID=MS.gene044581.t1.exon7;Parent=MS.gene044581.t1 |
| chr7.3 | CDS | 65882945 | 65883052 | 65882945 | ID=cds.MS.gene044581.t1;Parent=MS.gene044581.t1 |
| chr7.3 | exon | 65883804 | 65883893 | 65883804 | ID=MS.gene044581.t1.exon8;Parent=MS.gene044581.t1 |
| chr7.3 | CDS | 65883804 | 65883893 | 65883804 | ID=cds.MS.gene044581.t1;Parent=MS.gene044581.t1 |
| chr7.3 | exon | 65883982 | 65884082 | 65883982 | ID=MS.gene044581.t1.exon9;Parent=MS.gene044581.t1 |
| chr7.3 | CDS | 65883982 | 65884082 | 65883982 | ID=cds.MS.gene044581.t1;Parent=MS.gene044581.t1 |
| chr7.3 | exon | 65884917 | 65884956 | 65884917 | ID=MS.gene044581.t1.exon10;Parent=MS.gene044581.t1 |
| chr7.3 | CDS | 65884917 | 65884956 | 65884917 | ID=cds.MS.gene044581.t1;Parent=MS.gene044581.t1 |
| chr7.3 | exon | 65885085 | 65885243 | 65885085 | ID=MS.gene044581.t1.exon11;Parent=MS.gene044581.t1 |
| chr7.3 | CDS | 65885085 | 65885243 | 65885085 | ID=cds.MS.gene044581.t1;Parent=MS.gene044581.t1 |
| Gene Sequence |
| Protein sequence |
