AlfalfaGEDB | MS.gene045722

M. sativa cultivar XinJiangDaYe / MS.gene045722

KEGG: K02935
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00542.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045722.t1	GAU35832.1	89.4	216	23	0	1	216	1	216	7.40E-98	366.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045722.t1	P53163	40.4	146	80	3	78	216	49	194	4.2e-17	89.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045722.t1	A0A2K3P7T6	89.4	216	23	0	1	216	1	216	5.4e-98	366.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene68085	MS.gene045722	PPI
MS.gene045722	MS.gene60517	PPI
MS.gene005792	MS.gene045722	PPI
MS.gene045722	MS.gene09988	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045722.t1	MTR_2g064300	95.833	216	9	0	1	216	1	216	3.83e-143	398
MS.gene045722.t1	MTR_7g053520	83.871	217	34	1	1	216	1	217	3.36e-120	340
MS.gene045722.t1	MTR_2g031760	40.606	165	93	3	53	216	30	190	4.68e-33	118
MS.gene045722.t1	MTR_2g031960	47.857	140	66	3	79	216	21	155	7.82e-31	111
MS.gene045722.t1	MTR_8g076780	44.737	152	74	2	74	216	45	195	1.13e-29	109

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene045722.t1	AT1G70190	53.182	220	87	3	1	216	1	208	1.20e-65	201
MS.gene045722.t1	AT1G70190	53.182	220	87	3	1	216	1	208	1.20e-65	201
MS.gene045722.t1	AT4G36420	44.211	190	91	4	30	216	2	179	1.92e-34	121
MS.gene045722.t1	AT3G06040	41.611	149	80	1	68	216	45	186	5.70e-28	104
MS.gene045722.t1	AT3G06040	41.611	149	80	1	68	216	45	186	5.70e-28	104
MS.gene045722.t1	AT3G06040	41.611	149	80	1	68	216	45	186	5.70e-28	104
MS.gene045722.t1	AT4G37660	38.798	183	89	3	34	216	8	167	4.53e-25	96.7
MS.gene045722.t1	AT2G03130	57.500	80	34	0	137	216	12	91	1.12e-15	70.5

Find 54 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTGATGACTTTAATTT+TGG	0.115213	2.3:-30600056	None:intergenic
CCAGTTTATGAAAGCGTATT+AGG	0.115413	2.3:+30599632	MS.gene045722:CDS
TGGAGGGCCACGATTGTTAT+TGG	0.240332	2.3:-30599792	None:intergenic
GAAAGCAGGGTCTGCAAATT+TGG	0.254350	2.3:+30599937	MS.gene045722:CDS
GTGCGGGGCTTTACCGATTT+AGG	0.299345	2.3:+30600079	MS.gene045722:CDS
ACTAGCTGAAGGTGCGGAAT+TGG	0.312932	2.3:+30599859	MS.gene045722:CDS
CCTAATACGCTTTCATAAAC+TGG	0.317127	2.3:-30599632	None:intergenic
TGAGCTTGCTGTTGAGAATA+AGG	0.322760	2.3:+30599579	MS.gene045722:CDS
CAGTTTATGAAAGCGTATTA+GGG	0.345172	2.3:+30599633	MS.gene045722:CDS
TGCCTCCTTCAAACCTAAAT+CGG	0.380631	2.3:-30600092	None:intergenic
GGAGGGCCACGATTGTTATT+GGG	0.390326	2.3:-30599791	None:intergenic
GGAGGAAGACGTAGAGGAAG+TGG	0.397120	2.3:+30599718	MS.gene045722:CDS
ACTTGGTGCTAATGTTGTTT+TGG	0.411925	2.3:+30600201	MS.gene045722:CDS
CTAGCTGAAGGTGCGGAATT+GGG	0.425870	2.3:+30599860	MS.gene045722:CDS
TTATTGGGATCAAATGTTGC+AGG	0.430449	2.3:-30599776	None:intergenic
CTTTACCGATTTAGGTTTGA+AGG	0.453564	2.3:+30600087	MS.gene045722:CDS
TAAAGTCATCAAGGAGGTGC+GGG	0.464501	2.3:+30600063	MS.gene045722:CDS
GTCTGCAAATTTGGCCGGAA+TGG	0.467477	2.3:+30599946	MS.gene045722:CDS
GCTTTCATATATCCCACATT+GGG	0.475885	2.3:-30599920	None:intergenic
CTCCTCTTGGGCTGCAGTCG+TGG	0.476435	2.3:-30599978	None:intergenic
AAAGGTGTTTCAAAGGAAGA+AGG	0.492660	2.3:+30600151	MS.gene045722:CDS
GTTGCAGGATCAAAATCAGC+TGG	0.495579	2.3:-30599761	None:intergenic
TATAAAGAAAGGTGTTTCAA+AGG	0.503102	2.3:+30600144	MS.gene045722:CDS
ACACCGTCTATTATAAAGAA+AGG	0.512263	2.3:+30600133	MS.gene045722:CDS
TGCTTTCATATATCCCACAT+TGG	0.515923	2.3:-30599921	None:intergenic
TGGTCAAGTTGCAAGAAAAG+AGG	0.520139	2.3:+30599694	MS.gene045722:CDS
GCGTATTAGGGCTTAATGCT+AGG	0.525999	2.3:+30599645	MS.gene045722:CDS
ACACCTTTCTTTATAATAGA+CGG	0.526861	2.3:-30600136	None:intergenic
TTCCAAAATTAAAGTCATCA+AGG	0.526956	2.3:+30600054	MS.gene045722:CDS
AATGTGGGATATATGAAAGC+AGG	0.529942	2.3:+30599923	MS.gene045722:CDS
AGCCTGAAAACTTTCTCTGA+TGG	0.532734	2.3:-30599812	None:intergenic
CTGAAAACTTTCTCTGATGG+AGG	0.535890	2.3:-30599809	None:intergenic
GCAGGGTCTGCAAATTTGGC+CGG	0.545113	2.3:+30599941	MS.gene045722:CDS
TTTCAGGCTTGTTGATGAAG+TGG	0.545631	2.3:+30599826	MS.gene045722:CDS
AGAGGAGGAGGAAGACGTAG+AGG	0.548567	2.3:+30599712	MS.gene045722:CDS
AGTTGCAAGAAAAGAGGAGG+AGG	0.555547	2.3:+30599700	MS.gene045722:CDS
TTTAGGTTTGAAGGAGGCAA+AGG	0.562989	2.3:+30600096	MS.gene045722:CDS
TGAAAACTTTCTCTGATGGA+GGG	0.607842	2.3:-30599808	None:intergenic
GATAAAAGAAATGCCCAATG+TGG	0.608771	2.3:+30599907	MS.gene045722:CDS
TTAAAGTCATCAAGGAGGTG+CGG	0.612909	2.3:+30600062	MS.gene045722:CDS
ATAGAGAAAATGAAAGCACT+TGG	0.623385	2.3:+30600184	MS.gene045722:CDS
ATGTGGGATATATGAAAGCA+GGG	0.623877	2.3:+30599924	MS.gene045722:CDS
ATAAAAGAAATGCCCAATGT+GGG	0.625462	2.3:+30599908	MS.gene045722:CDS
TACCGATTTAGGTTTGAAGG+AGG	0.626116	2.3:+30600090	MS.gene045722:CDS
CACGACTGCAGCCCAAGAGG+AGG	0.628136	2.3:+30599979	MS.gene045722:CDS
TTGCAGGATCAAAATCAGCT+GGG	0.640796	2.3:-30599760	None:intergenic
AGCCACGACTGCAGCCCAAG+AGG	0.642383	2.3:+30599976	MS.gene045722:CDS
GCGTCTCTAACACTAGCTGA+AGG	0.646060	2.3:+30599848	MS.gene045722:CDS
ATAAGGCATCGTTTACACAA+TGG	0.647228	2.3:+30599596	MS.gene045722:CDS
TCAAGTTGCAAGAAAAGAGG+AGG	0.661992	2.3:+30599697	MS.gene045722:CDS
AAAGTCATCAAGGAGGTGCG+GGG	0.667732	2.3:+30600064	MS.gene045722:CDS
TTTGATCCCAATAACAATCG+TGG	0.681002	2.3:+30599785	MS.gene045722:CDS
TCTAACACTAGCTGAAGGTG+CGG	0.686308	2.3:+30599853	MS.gene045722:CDS
CAAAATTAAAGTCATCAAGG+AGG	0.704497	2.3:+30600057	MS.gene045722:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTCAAATACAGTTTTTTC+TGG	-	chr2.3:30600010-30600029	None:intergenic	20.0%
!	ACACCTTTCTTTATAATAGA+CGG	-	chr2.3:30600139-30600158	None:intergenic	25.0%
!	TTCCAAAATTAAAGTCATCA+AGG	+	chr2.3:30600054-30600073	MS.gene045722:CDS	25.0%
!!!	AATGTATTTTCGAGAAACTT+TGG	+	chr2.3:30599674-30599693	MS.gene045722:CDS	25.0%
!!!	CTCATTTTGATGAAGAAAAT+GGG	+	chr2.3:30599884-30599903	MS.gene045722:CDS	25.0%
!!!	TATAAAGAAAGGTGTTTCAA+AGG	+	chr2.3:30600144-30600163	MS.gene045722:CDS	25.0%
!!!	TCATTTTGATGAAGAAAATG+GGG	+	chr2.3:30599885-30599904	MS.gene045722:CDS	25.0%
!!!	TCTCATTTTGATGAAGAAAA+TGG	+	chr2.3:30599883-30599902	MS.gene045722:CDS	25.0%
	ACACCGTCTATTATAAAGAA+AGG	+	chr2.3:30600133-30600152	MS.gene045722:CDS	30.0%
	ATAAAAGAAATGCCCAATGT+GGG	+	chr2.3:30599908-30599927	MS.gene045722:CDS	30.0%
	CAAAATTAAAGTCATCAAGG+AGG	+	chr2.3:30600057-30600076	MS.gene045722:CDS	30.0%
!	CAGTTTATGAAAGCGTATTA+GGG	+	chr2.3:30599633-30599652	MS.gene045722:CDS	30.0%
!!	ATAGAGAAAATGAAAGCACT+TGG	+	chr2.3:30600184-30600203	MS.gene045722:CDS	30.0%
!!	CTCCTTGATGACTTTAATTT+TGG	-	chr2.3:30600059-30600078	None:intergenic	30.0%
	AATGTGGGATATATGAAAGC+AGG	+	chr2.3:30599923-30599942	MS.gene045722:CDS	35.0%
	ATGTGGGATATATGAAAGCA+GGG	+	chr2.3:30599924-30599943	MS.gene045722:CDS	35.0%
	CCTAATACGCTTTCATAAAC+TGG	-	chr2.3:30599635-30599654	None:intergenic	35.0%
	GATAAAAGAAATGCCCAATG+TGG	+	chr2.3:30599907-30599926	MS.gene045722:CDS	35.0%
	GCTTTCATATATCCCACATT+GGG	-	chr2.3:30599923-30599942	None:intergenic	35.0%
	TGAAAACTTTCTCTGATGGA+GGG	-	chr2.3:30599811-30599830	None:intergenic	35.0%
	TGCTTTCATATATCCCACAT+TGG	-	chr2.3:30599924-30599943	None:intergenic	35.0%
	TTATTGGGATCAAATGTTGC+AGG	-	chr2.3:30599779-30599798	None:intergenic	35.0%
	TTTGATCCCAATAACAATCG+TGG	+	chr2.3:30599785-30599804	MS.gene045722:CDS	35.0%
!	CCAGTTTATGAAAGCGTATT+AGG	+	chr2.3:30599632-30599651	MS.gene045722:CDS	35.0%
!!	AAAGGTGTTTCAAAGGAAGA+AGG	+	chr2.3:30600151-30600170	MS.gene045722:CDS	35.0%
!!	ATAAGGCATCGTTTACACAA+TGG	+	chr2.3:30599596-30599615	MS.gene045722:CDS	35.0%
!!	CTTTACCGATTTAGGTTTGA+AGG	+	chr2.3:30600087-30600106	MS.gene045722:CDS	35.0%
!!!	ACTTGGTGCTAATGTTGTTT+TGG	+	chr2.3:30600201-30600220	MS.gene045722:CDS	35.0%
	AGCCTGAAAACTTTCTCTGA+TGG	-	chr2.3:30599815-30599834	None:intergenic	40.0%
	CTGAAAACTTTCTCTGATGG+AGG	-	chr2.3:30599812-30599831	None:intergenic	40.0%
	TGCCTCCTTCAAACCTAAAT+CGG	-	chr2.3:30600095-30600114	None:intergenic	40.0%
	TTAAAGTCATCAAGGAGGTG+CGG	+	chr2.3:30600062-30600081	MS.gene045722:CDS	40.0%
	TTGCAGGATCAAAATCAGCT+GGG	-	chr2.3:30599763-30599782	None:intergenic	40.0%
!	CTCCATCAGAGAAAGTTTTC+AGG	+	chr2.3:30599810-30599829	MS.gene045722:CDS	40.0%
!	TCAAGTTGCAAGAAAAGAGG+AGG	+	chr2.3:30599697-30599716	MS.gene045722:CDS	40.0%
!	TGGTCAAGTTGCAAGAAAAG+AGG	+	chr2.3:30599694-30599713	MS.gene045722:CDS	40.0%
!	TTTAGGTTTGAAGGAGGCAA+AGG	+	chr2.3:30600096-30600115	MS.gene045722:CDS	40.0%
!!	TACCGATTTAGGTTTGAAGG+AGG	+	chr2.3:30600090-30600109	MS.gene045722:CDS	40.0%
!!	TGAGCTTGCTGTTGAGAATA+AGG	+	chr2.3:30599579-30599598	MS.gene045722:CDS	40.0%
!!	TTTCAGGCTTGTTGATGAAG+TGG	+	chr2.3:30599826-30599845	MS.gene045722:CDS	40.0%
!!!	TTCTGGTTTTACCTCCTCTT+GGG	-	chr2.3:30599993-30600012	None:intergenic	40.0%
!!!	TTTCTGGTTTTACCTCCTCT+TGG	-	chr2.3:30599994-30600013	None:intergenic	40.0%
	AGTTGCAAGAAAAGAGGAGG+AGG	+	chr2.3:30599700-30599719	MS.gene045722:CDS	45.0%
	GAAAGCAGGGTCTGCAAATT+TGG	+	chr2.3:30599937-30599956	MS.gene045722:CDS	45.0%
	GCGTATTAGGGCTTAATGCT+AGG	+	chr2.3:30599645-30599664	MS.gene045722:CDS	45.0%
	GTTGCAGGATCAAAATCAGC+TGG	-	chr2.3:30599764-30599783	None:intergenic	45.0%
	TCTAACACTAGCTGAAGGTG+CGG	+	chr2.3:30599853-30599872	MS.gene045722:CDS	45.0%
!	TAAAGTCATCAAGGAGGTGC+GGG	+	chr2.3:30600063-30600082	MS.gene045722:CDS	45.0%
	GCGTCTCTAACACTAGCTGA+AGG	+	chr2.3:30599848-30599867	MS.gene045722:CDS	50.0%
	GTCTGCAAATTTGGCCGGAA+TGG	+	chr2.3:30599946-30599965	MS.gene045722:CDS	50.0%
	TGGAGGGCCACGATTGTTAT+TGG	-	chr2.3:30599795-30599814	None:intergenic	50.0%
!	AAAGTCATCAAGGAGGTGCG+GGG	+	chr2.3:30600064-30600083	MS.gene045722:CDS	50.0%
!	ACTAGCTGAAGGTGCGGAAT+TGG	+	chr2.3:30599859-30599878	MS.gene045722:CDS	50.0%
!	CGTGGCTTTTGTTGCCATTC+CGG	-	chr2.3:30599963-30599982	None:intergenic	50.0%
!	CTAGCTGAAGGTGCGGAATT+GGG	+	chr2.3:30599860-30599879	MS.gene045722:CDS	50.0%
!	GGAGGGCCACGATTGTTATT+GGG	-	chr2.3:30599794-30599813	None:intergenic	50.0%
	AGAGGAGGAGGAAGACGTAG+AGG	+	chr2.3:30599712-30599731	MS.gene045722:CDS	55.0%
	GCAGGGTCTGCAAATTTGGC+CGG	+	chr2.3:30599941-30599960	MS.gene045722:CDS	55.0%
	GGAGGAAGACGTAGAGGAAG+TGG	+	chr2.3:30599718-30599737	MS.gene045722:CDS	55.0%
!!	GTGCGGGGCTTTACCGATTT+AGG	+	chr2.3:30600079-30600098	MS.gene045722:CDS	55.0%
	AGCCACGACTGCAGCCCAAG+AGG	+	chr2.3:30599976-30599995	MS.gene045722:CDS	65.0%
	CACGACTGCAGCCCAAGAGG+AGG	+	chr2.3:30599979-30599998	MS.gene045722:CDS	65.0%
	CTCCTCTTGGGCTGCAGTCG+TGG	-	chr2.3:30599981-30600000	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	30599578	30600228	30599578	ID=MS.gene045722
chr2.3	mRNA	30599578	30600228	30599578	ID=MS.gene045722.t1;Parent=MS.gene045722
chr2.3	exon	30599578	30600228	30599578	ID=MS.gene045722.t1.exon1;Parent=MS.gene045722.t1
chr2.3	CDS	30599578	30600228	30599578	ID=cds.MS.gene045722.t1;Parent=MS.gene045722.t1

Gene Sequence

>MS.gene045722

ATGAGCTTGCTGTTGAGAATAAGGCATCGTTTACACAATGGTTTGTATAGACAACCAGTTTATGAAAGCGTATTAGGGCTTAATGCTAGGAAATTAAATGTATTTTCGAGAAACTTTGGTCAAGTTGCAAGAAAAGAGGAGGAGGAAGACGTAGAGGAAGTGGAAATTGATCAAAGAAGTCTCCCAGCTGATTTTGATCCTGCAACATTTGATCCCAATAACAATCGTGGCCCTCCATCAGAGAAAGTTTTCAGGCTTGTTGATGAAGTGGCGTCTCTAACACTAGCTGAAGGTGCGGAATTGGGTCTCATTTTGATGAAGAAAATGGGGATAAAAGAAATGCCCAATGTGGGATATATGAAAGCAGGGTCTGCAAATTTGGCCGGAATGGCAACAAAAGCCACGACTGCAGCCCAAGAGGAGGTAAAACCAGAAAAAACTGTATTTGAATTGAAACTGTTGTCGTATGAAGCAGCTTCCAAAATTAAAGTCATCAAGGAGGTGCGGGGCTTTACCGATTTAGGTTTGAAGGAGGCAAAGGATATTGTCGAAAAAACACCGTCTATTATAAAGAAAGGTGTTTCAAAGGAAGAAGGAGAACAGATAATAGAGAAAATGAAAGCACTTGGTGCTAATGTTGTTTTGGAGTGA

Protein sequence

>MS.gene045722.t1

MSLLLRIRHRLHNGLYRQPVYESVLGLNARKLNVFSRNFGQVARKEEEEDVEEVEIDQRSLPADFDPATFDPNNNRGPPSEKVFRLVDEVASLTLAEGAELGLILMKKMGIKEMPNVGYMKAGSANLAGMATKATTAAQEEVKPEKTVFELKLLSYEAASKIKVIKEVRGFTDLGLKEAKDIVEKTPSIIKKGVSKEEGEQIIEKMKALGANVVLE