AlfalfaGEDB | MS.gene047221

M. sativa cultivar XinJiangDaYe / MS.gene047221

KEGG: -
GO: -
PFAM: PF05078.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047221.t1	GAU24406.1	81.6	185	26	3	42	226	53	229	1.30E-66	263.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047221.t1	Q9STW3	55.3	179	70	3	44	222	39	207	5.0e-48	192.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047221.t1	A0A2Z6LYX5	81.6	185	26	3	42	226	53	229	9.1e-67	263.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene047359	MS.gene047221	PPI
MS.gene43093	MS.gene047221	PPI
MS.gene01477	MS.gene047221	PPI
MS.gene004174	MS.gene047221	PPI
MS.gene047221	MS.gene32027	PPI
MS.gene047221	MS.gene002504	PPI
MS.gene02809	MS.gene047221	PPI
MS.gene02810	MS.gene047221	PPI
MS.gene39522	MS.gene047221	PPI
MS.gene72970	MS.gene047221	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047221.t1	MTR_4g088335	47.917	192	86	4	32	222	26	204	2.75e-60	189
MS.gene047221.t1	MTR_2g014550	48.187	193	85	3	33	222	28	208	3.62e-60	188
MS.gene047221.t1	MTR_2g014520	47.120	191	89	2	32	222	27	205	1.05e-58	184
MS.gene047221.t1	MTR_4g078870	43.784	185	89	4	40	222	32	203	1.50e-48	159
MS.gene047221.t1	MTR_2g020850	40.000	190	99	3	33	222	13	187	2.46e-43	145
MS.gene047221.t1	MTR_5g044580	39.683	189	99	4	33	218	38	214	1.65e-41	141
MS.gene047221.t1	MTR_7g010890	39.153	189	100	4	33	218	38	214	1.94e-39	135
MS.gene047221.t1	MTR_4g118480	37.186	199	111	4	24	222	11	195	6.23e-38	132
MS.gene047221.t1	MTR_4g118470	39.344	183	97	4	40	222	29	197	8.69e-38	131

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene047221.t1	AT3G02430	52.535	217	93	3	6	222	7	213	1.95e-71	218
MS.gene047221.t1	AT4G24310	53.394	221	84	4	2	222	6	207	2.62e-70	214
MS.gene047221.t1	AT4G18425	49.153	177	76	4	47	222	48	211	1.05e-53	172
MS.gene047221.t1	AT5G46090	45.078	193	92	4	31	222	33	212	3.03e-52	168
MS.gene047221.t1	AT4G28485	44.670	197	94	5	29	222	14	198	5.07e-45	149
MS.gene047221.t1	AT3G21550	40.957	188	94	3	45	232	14	184	2.26e-39	134
MS.gene047221.t1	AT1G09157	38.889	180	108	2	40	218	61	239	4.98e-37	130
MS.gene047221.t1	AT3G21520	37.173	191	104	4	43	232	26	201	1.10e-36	128
MS.gene047221.t1	AT5G39650	38.889	180	108	2	40	218	62	240	4.73e-36	128
MS.gene047221.t1	AT5G27370	37.705	183	103	5	43	218	8	186	1.88e-29	109
MS.gene047221.t1	AT4G28485	44.545	110	50	2	113	222	65	163	5.60e-20	84.0

Find 59 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTTCAGATTGCTTATTAT+TGG	0.102422	2.2:-73642897	None:intergenic
GTACTCCTAGTAATTATATT+TGG	0.194804	2.2:-73643566	None:intergenic
CTAGTAATTATATTTGGAAT+TGG	0.267098	2.2:-73643560	None:intergenic
TGTTCTTTGCTGTTGCATTA+AGG	0.305960	2.2:+73643368	MS.gene047221:CDS
GCAGCAGGTGCAGGTGTAAC+AGG	0.319770	2.2:-73643532	None:intergenic
GATATTGTGCCCCTCGGAAT+TGG	0.326356	2.2:+73643451	MS.gene047221:CDS
TGAAGACTGGCGTGAGAATC+TGG	0.355118	2.2:-73643060	None:intergenic
TAGTCACACGGCGTGGTTGC+GGG	0.359159	2.2:-73643417	None:intergenic
GTTCTTTGCTGTTGCATTAA+GGG	0.365115	2.2:+73643369	MS.gene047221:CDS
ATGCTTCCTTGCTTGTTTCA+CGG	0.371761	2.2:+73643153	MS.gene047221:CDS
TAGTAATTATATTTGGAATT+GGG	0.375012	2.2:-73643559	None:intergenic
CCATGGCATCGCAACCTTCA+AGG	0.392282	2.2:+73643210	MS.gene047221:CDS
GTAGTATCTGGAGCATTAAC+GGG	0.406461	2.2:+73642989	MS.gene047221:CDS
AAATGCATGTACAAAGTCAA+TGG	0.433016	2.2:-73643332	None:intergenic
AGTAGTATCTGGAGCATTAA+CGG	0.435471	2.2:+73642988	MS.gene047221:CDS
CGACGGTCAGTAGTAGTATC+TGG	0.437746	2.2:+73642977	MS.gene047221:CDS
TTAGTCACACGGCGTGGTTG+CGG	0.444013	2.2:-73643418	None:intergenic
TTGGAATTGGGTGCAGCAGC+AGG	0.459846	2.2:-73643547	None:intergenic
TTGGGTGCAGCAGCAGGTGC+AGG	0.468002	2.2:-73643541	None:intergenic
TCGCAACCTTCAAGGGTCTC+TGG	0.469635	2.2:+73643218	MS.gene047221:CDS
GCAGCAGCACATGGAATTAG+TGG	0.477735	2.2:+73642941	MS.gene047221:CDS
CAATTCCAAATATAATTACT+AGG	0.486361	2.2:+73643561	MS.gene047221:CDS
GCAACCACGCCGTGTGACTA+AGG	0.489093	2.2:+73643420	MS.gene047221:CDS
CATGGCATCGCAACCTTCAA+GGG	0.499059	2.2:+73643211	MS.gene047221:CDS
GCTCCGTTGTTTGTGAAGAC+TGG	0.503057	2.2:-73643073	None:intergenic
AAATAGCCAGAGACCCTTGA+AGG	0.509853	2.2:-73643224	None:intergenic
CCAACCACTAGACATGGGAT+TGG	0.514072	2.2:+73643505	MS.gene047221:CDS
TTATACTTGCTCAACTCAGC+TGG	0.520275	2.2:-73643301	None:intergenic
GGGAAGTAAGTTTGCCAAGT+TGG	0.532415	2.2:-73643017	None:intergenic
AATGCATGTACAAAGTCAAT+GGG	0.533496	2.2:-73643331	None:intergenic
GCGTGAGAATCTGGAATGCT+AGG	0.537937	2.2:-73643051	None:intergenic
ATTAACGGGGACAGCCAACT+TGG	0.539398	2.2:+73643003	MS.gene047221:CDS
AATAAGCAATCTGAAGCAGA+TGG	0.548858	2.2:+73642902	MS.gene047221:CDS
TCCATGACATTGACATTGAC+GGG	0.550083	2.2:-73643256	None:intergenic
AGTTGAGCAAGTATAAGATC+AGG	0.557038	2.2:+73643308	MS.gene047221:CDS
CCAATCCCATGTCTAGTGGT+TGG	0.561333	2.2:-73643505	None:intergenic
GTCCATGACATTGACATTGA+CGG	0.563837	2.2:-73643257	None:intergenic
GTTCTTGATATTGTGCCCCT+CGG	0.569071	2.2:+73643445	MS.gene047221:CDS
CCTTGAAGGTTGCGATGCCA+TGG	0.569779	2.2:-73643210	None:intergenic
ACCCGTCAATGTCAATGTCA+TGG	0.570259	2.2:+73643255	MS.gene047221:CDS
ACAGTGAATAATGCATCAGA+TGG	0.586121	2.2:+73643178	MS.gene047221:CDS
TTGGCAAACTTACTTCCCAC+AGG	0.590824	2.2:+73643022	MS.gene047221:CDS
GAATGCTAGGAGTGTTCCTG+TGG	0.592747	2.2:-73643038	None:intergenic
CAGCAGGTGCAGGTGTAACA+GGG	0.600653	2.2:-73643531	None:intergenic
ATGAGATTGCAAGCAAGATG+AGG	0.612498	2.2:-73643132	None:intergenic
GTAGCCAATCCCATGTCTAG+TGG	0.615835	2.2:-73643509	None:intergenic
AAGTGTGAGAATGCGAGTAA+CGG	0.616907	2.2:-73643104	None:intergenic
GCATAGGAATCCAATTCCGA+GGG	0.617670	2.2:-73643461	None:intergenic
TGCATAGGAATCCAATTCCG+AGG	0.619320	2.2:-73643462	None:intergenic
CAAGAACCTCCTTAGTCACA+CGG	0.627426	2.2:-73643429	None:intergenic
CTGTGTCCGTGAAACAAGCA+AGG	0.639798	2.2:-73643159	None:intergenic
ACCTCCTTAGTCACACGGCG+TGG	0.648095	2.2:-73643424	None:intergenic
ACGCCAGTCTTCACAAACAA+CGG	0.649231	2.2:+73643070	MS.gene047221:CDS
GTGGTAGACGACGACGACGA+CGG	0.670485	2.2:+73642960	MS.gene047221:CDS
TAGTATCTGGAGCATTAACG+GGG	0.687598	2.2:+73642990	MS.gene047221:CDS
TCAGATGGAAGAATCTACCA+TGG	0.695565	2.2:+73643193	MS.gene047221:CDS
ACCACGCCGTGTGACTAAGG+AGG	0.698128	2.2:+73643423	MS.gene047221:CDS
AATGCTAGGAGTGTTCCTGT+GGG	0.719785	2.2:-73643037	None:intergenic
CATAGGAATCCAATTCCGAG+GGG	0.764587	2.2:-73643460	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAGTAATTATATTTGGAATT+GGG	-	chr2.2:73643562-73643581	None:intergenic	15.0%
!!!	CTAGTAATTATATTTGGAAT+TGG	-	chr2.2:73643563-73643582	None:intergenic	20.0%
	AAATGCATGTACAAAGTCAA+TGG	-	chr2.2:73643335-73643354	None:intergenic	30.0%
	AATGCATGTACAAAGTCAAT+GGG	-	chr2.2:73643334-73643353	None:intergenic	30.0%
	TGCTTCAGATTGCTTATTAT+TGG	-	chr2.2:73642900-73642919	None:intergenic	30.0%
	ACAGTGAATAATGCATCAGA+TGG	+	chr2.2:73643178-73643197	MS.gene047221:CDS	35.0%
	AGTTGAGCAAGTATAAGATC+AGG	+	chr2.2:73643308-73643327	MS.gene047221:CDS	35.0%
!	AATAAGCAATCTGAAGCAGA+TGG	+	chr2.2:73642902-73642921	MS.gene047221:CDS	35.0%
!	AGTAGTATCTGGAGCATTAA+CGG	+	chr2.2:73642988-73643007	MS.gene047221:CDS	35.0%
!	TTTTTCCAACCACTAGACAT+GGG	+	chr2.2:73643500-73643519	MS.gene047221:CDS	35.0%
!!	GTTCTTTGCTGTTGCATTAA+GGG	+	chr2.2:73643369-73643388	MS.gene047221:CDS	35.0%
!!	TGTTCTTTGCTGTTGCATTA+AGG	+	chr2.2:73643368-73643387	MS.gene047221:CDS	35.0%
	AAGTGTGAGAATGCGAGTAA+CGG	-	chr2.2:73643107-73643126	None:intergenic	40.0%
	ATGAGATTGCAAGCAAGATG+AGG	-	chr2.2:73643135-73643154	None:intergenic	40.0%
	ATGCTTCCTTGCTTGTTTCA+CGG	+	chr2.2:73643153-73643172	MS.gene047221:CDS	40.0%
	CGACAAAAAGCAAAGTGCAT+AGG	-	chr2.2:73643480-73643499	None:intergenic	40.0%
	GTCCATGACATTGACATTGA+CGG	-	chr2.2:73643260-73643279	None:intergenic	40.0%
	TCAGATGGAAGAATCTACCA+TGG	+	chr2.2:73643193-73643212	MS.gene047221:CDS	40.0%
	TCCATGACATTGACATTGAC+GGG	-	chr2.2:73643259-73643278	None:intergenic	40.0%
	TTATACTTGCTCAACTCAGC+TGG	-	chr2.2:73643304-73643323	None:intergenic	40.0%
!	GTAGTATCTGGAGCATTAAC+GGG	+	chr2.2:73642989-73643008	MS.gene047221:CDS	40.0%
!	GTTTTTCCAACCACTAGACA+TGG	+	chr2.2:73643499-73643518	MS.gene047221:CDS	40.0%
!	TAGTATCTGGAGCATTAACG+GGG	+	chr2.2:73642990-73643009	MS.gene047221:CDS	40.0%
	AAATAGCCAGAGACCCTTGA+AGG	-	chr2.2:73643227-73643246	None:intergenic	45.0%
	ACCCGTCAATGTCAATGTCA+TGG	+	chr2.2:73643255-73643274	MS.gene047221:CDS	45.0%
	ACGCCAGTCTTCACAAACAA+CGG	+	chr2.2:73643070-73643089	MS.gene047221:CDS	45.0%
	CAAGAACCTCCTTAGTCACA+CGG	-	chr2.2:73643432-73643451	None:intergenic	45.0%
	CATAGGAATCCAATTCCGAG+GGG	-	chr2.2:73643463-73643482	None:intergenic	45.0%
	GCATAGGAATCCAATTCCGA+GGG	-	chr2.2:73643464-73643483	None:intergenic	45.0%
	GGGAAGTAAGTTTGCCAAGT+TGG	-	chr2.2:73643020-73643039	None:intergenic	45.0%
	GTTCTTGATATTGTGCCCCT+CGG	+	chr2.2:73643445-73643464	MS.gene047221:CDS	45.0%
	TGCATAGGAATCCAATTCCG+AGG	-	chr2.2:73643465-73643484	None:intergenic	45.0%
	TTGGCAAACTTACTTCCCAC+AGG	+	chr2.2:73643022-73643041	MS.gene047221:CDS	45.0%
!!	AATGCTAGGAGTGTTCCTGT+GGG	-	chr2.2:73643040-73643059	None:intergenic	45.0%
	ATTAACGGGGACAGCCAACT+TGG	+	chr2.2:73643003-73643022	MS.gene047221:CDS	50.0%
	CATGGCATCGCAACCTTCAA+GGG	+	chr2.2:73643211-73643230	MS.gene047221:CDS	50.0%
	CCAACCACTAGACATGGGAT+TGG	+	chr2.2:73643505-73643524	MS.gene047221:CDS	50.0%
	CCAATCCCATGTCTAGTGGT+TGG	-	chr2.2:73643508-73643527	None:intergenic	50.0%
	CGACGGTCAGTAGTAGTATC+TGG	+	chr2.2:73642977-73642996	MS.gene047221:CDS	50.0%
	CTGTGTCCGTGAAACAAGCA+AGG	-	chr2.2:73643162-73643181	None:intergenic	50.0%
	GATATTGTGCCCCTCGGAAT+TGG	+	chr2.2:73643451-73643470	MS.gene047221:CDS	50.0%
	GCAGCAGCACATGGAATTAG+TGG	+	chr2.2:73642941-73642960	MS.gene047221:CDS	50.0%
	GCTCCGTTGTTTGTGAAGAC+TGG	-	chr2.2:73643076-73643095	None:intergenic	50.0%
	GTAGCCAATCCCATGTCTAG+TGG	-	chr2.2:73643512-73643531	None:intergenic	50.0%
	TGAAGACTGGCGTGAGAATC+TGG	-	chr2.2:73643063-73643082	None:intergenic	50.0%
!	GCGTGAGAATCTGGAATGCT+AGG	-	chr2.2:73643054-73643073	None:intergenic	50.0%
!!	GAATGCTAGGAGTGTTCCTG+TGG	-	chr2.2:73643041-73643060	None:intergenic	50.0%
	CAGCAGGTGCAGGTGTAACA+GGG	-	chr2.2:73643534-73643553	None:intergenic	55.0%
	CCATGGCATCGCAACCTTCA+AGG	+	chr2.2:73643210-73643229	MS.gene047221:CDS	55.0%
	CCTTGAAGGTTGCGATGCCA+TGG	-	chr2.2:73643213-73643232	None:intergenic	55.0%
	TCGCAACCTTCAAGGGTCTC+TGG	+	chr2.2:73643218-73643237	MS.gene047221:CDS	55.0%
	TTAGTCACACGGCGTGGTTG+CGG	-	chr2.2:73643421-73643440	None:intergenic	55.0%
	TTGGAATTGGGTGCAGCAGC+AGG	-	chr2.2:73643550-73643569	None:intergenic	55.0%
	ACCACGCCGTGTGACTAAGG+AGG	+	chr2.2:73643423-73643442	MS.gene047221:CDS	60.0%
	ACCTCCTTAGTCACACGGCG+TGG	-	chr2.2:73643427-73643446	None:intergenic	60.0%
	GCAACCACGCCGTGTGACTA+AGG	+	chr2.2:73643420-73643439	MS.gene047221:CDS	60.0%
	GCAGCAGGTGCAGGTGTAAC+AGG	-	chr2.2:73643535-73643554	None:intergenic	60.0%
	GTGGTAGACGACGACGACGA+CGG	+	chr2.2:73642960-73642979	MS.gene047221:CDS	60.0%
	TAGTCACACGGCGTGGTTGC+GGG	-	chr2.2:73643420-73643439	None:intergenic	60.0%
	TCAGCAGCAGCAGCAGCACA+TGG	+	chr2.2:73642932-73642951	MS.gene047221:CDS	60.0%
	TTGGGTGCAGCAGCAGGTGC+AGG	-	chr2.2:73643544-73643563	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	73642875	73643582	73642875	ID=MS.gene047221
chr2.2	mRNA	73642875	73643582	73642875	ID=MS.gene047221.t1;Parent=MS.gene047221
chr2.2	exon	73642875	73643582	73642875	ID=MS.gene047221.t1.exon1;Parent=MS.gene047221.t1
chr2.2	CDS	73642875	73643582	73642875	ID=cds.MS.gene047221.t1;Parent=MS.gene047221.t1

Gene Sequence

>MS.gene047221

ATGTCGGTTACACAACGTCGATCCAATAATAAGCAATCTGAAGCAGATGGATCATCATCAGCAGCAGCAGCAGCACATGGAATTAGTGGTAGACGACGACGACGACGGTCAGTAGTAGTATCTGGAGCATTAACGGGGACAGCCAACTTGGCAAACTTACTTCCCACAGGAACACTCCTAGCATTCCAGATTCTCACGCCAGTCTTCACAAACAACGGAGCTTGTGACTCCGTTACTCGCATTCTCACACTTGTACTCCTCATCTTGCTTGCAATCTCATGCTTCCTTGCTTGTTTCACGGACACAGTGAATAATGCATCAGATGGAAGAATCTACCATGGCATCGCAACCTTCAAGGGTCTCTGGCTATTTGATTACTCACCCGTCAATGTCAATGTCATGGACAGTAGTAGTACAATAATATTACCAGCTGAGTTGAGCAAGTATAAGATCAGGCCCATTGACTTTGTACATGCATTTTTGTCTGTCTTTGTGTTCTTTGCTGTTGCATTAAGGGATAACAATGTGTTGACATGTTATTACCCGCAACCACGCCGTGTGACTAAGGAGGTTCTTGATATTGTGCCCCTCGGAATTGGATTCCTATGCACTTTGCTTTTTGTCGTTTTTCCAACCACTAGACATGGGATTGGCTACCCTGTTACACCTGCACCTGCTGCTGCACCCAATTCCAAATATAATTACTAG

Protein sequence

>MS.gene047221.t1

MSVTQRRSNNKQSEADGSSSAAAAAHGISGRRRRRRSVVVSGALTGTANLANLLPTGTLLAFQILTPVFTNNGACDSVTRILTLVLLILLAISCFLACFTDTVNNASDGRIYHGIATFKGLWLFDYSPVNVNVMDSSSTIILPAELSKYKIRPIDFVHAFLSVFVFFAVALRDNNVLTCYYPQPRRVTKEVLDIVPLGIGFLCTLLFVVFPTTRHGIGYPVTPAPAAAPNSKYNY