AlfalfaGEDB | MS.gene049156

M. sativa cultivar XinJiangDaYe / MS.gene049156

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008150 GO:0009987 GO:0016043 GO:0016787 GO:0016788 GO:0030599 GO:0042545 GO:0045229 GO:0052689 GO:0071554 GO:0071555 GO:0071840
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049156.t1	RHN43755.1	94.8	229	12	0	1	229	313	541	6.20E-127	463.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049156.t1	O04887	66.8	229	75	1	1	229	283	510	2.2e-93	343.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049156.t1	A0A072TX14	94.8	229	12	0	1	229	144	372	4.5e-127	463.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049156	MS.gene063695	0.820948	5.24E-53	-1.69E-46
MS.gene049156	MS.gene063696	0.822237	2.64E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 60 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGCTGATTCTTGGATTTC+AGG	0.130565	4.4:+2361476	MS.gene049156:CDS
AATAATTATTGCAGCTGTTA+TGG	0.230375	4.4:+2360824	None:intergenic
GCATTGAGTACACTTTATTA+TGG	0.267204	4.4:+2361338	MS.gene049156:CDS
CTGCTGATTCTTGGATTTCA+GGG	0.308239	4.4:+2361477	MS.gene049156:CDS
AATCATTTGCTGCGGTTATA+CGG	0.315468	4.4:-2361181	None:intergenic
ATAATTATTGCAGCTGTTAT+GGG	0.345443	4.4:+2360825	None:intergenic
GGAACTATTGATTTCATCTT+CGG	0.369134	4.4:+2361029	MS.gene049156:CDS
TTTGATAAATTCACAAGGTT+GGG	0.373566	4.4:+2361295	MS.gene049156:CDS
AATTCAGTTAAAACATATCT+TGG	0.398697	4.4:+2361218	MS.gene049156:CDS
TGTTGCAGTCGCGGTAGAAT+TGG	0.411073	4.4:-2361001	None:intergenic
TGTTCTTCCTTGTGCTGTTA+CGG	0.414972	4.4:-2361120	None:intergenic
ATACTGGTGGAAGAGTTAAT+TGG	0.417090	4.4:+2361390	MS.gene049156:CDS
GTAATGAAAAGTAATATTGA+TGG	0.420796	4.4:+2361272	MS.gene049156:CDS
GTTTGATAAATTCACAAGGT+TGG	0.422089	4.4:+2361294	MS.gene049156:CDS
GCGGTTGGCGTAAGCATATA+AGG	0.426322	4.4:-2360979	None:intergenic
CGCAGCAAATGATTTGAAAC+CGG	0.431620	4.4:+2361190	MS.gene049156:CDS
GTTGGTGGTGGTGCTAATAC+TGG	0.437055	4.4:+2361374	MS.gene049156:CDS
CAAGCAGTAGCTCTCCGTTC+CGG	0.442034	4.4:+2360906	MS.gene049156:CDS
GGTGTCTTGGTAGCCTTTGA+AGG	0.444283	4.4:-2360958	None:intergenic
GTGTTCTGTTGGTTGCTCAT+TGG	0.453378	4.4:-2361098	None:intergenic
ACAAGGTTGGGCTCCATGGA+TGG	0.456078	4.4:+2361307	MS.gene049156:CDS
CAAGGTTGGGCTCCATGGAT+GGG	0.470284	4.4:+2361308	MS.gene049156:CDS
ATTCACAAGGTTGGGCTCCA+TGG	0.471108	4.4:+2361303	MS.gene049156:CDS
ATTGCAGCTGTTATGGGAGA+TGG	0.472604	4.4:+2360831	None:intergenic
GGACATGACCTTTGAGAATA+CGG	0.477411	4.4:+2360866	MS.gene049156:CDS
TTCTACCGTTGTGCCTTCAA+AGG	0.487774	4.4:+2360945	MS.gene049156:CDS
AGTGGTGTGCCGTTTGATGC+AGG	0.508356	4.4:+2361500	MS.gene049156:CDS
TGCAGTCGCGGTAGAATTGG+CGG	0.511251	4.4:-2360998	None:intergenic
GTTCTTGAATATTTCTGCCA+TGG	0.518439	4.4:-2361245	None:intergenic
GGTGGAAGAGTTAATTGGCC+TGG	0.520232	4.4:+2361395	MS.gene049156:CDS
GGGAGATGGTTTCATAGCTA+AGG	0.523196	4.4:+2360845	MS.gene049156:CDS
ATTCACAAACCTGCATCAAA+CGG	0.526344	4.4:-2361509	None:intergenic
TACCGCGACTGCAACATCTA+CGG	0.528232	4.4:+2361008	MS.gene049156:CDS
CGTAAGCATATAAGGTGTCT+TGG	0.533953	4.4:-2360971	None:intergenic
GGTTGGGCTCCATGGATGGG+TGG	0.536463	4.4:+2361311	MS.gene049156:CDS
TGTTATTACTAATCCTTCTG+AGG	0.537907	4.4:+2361424	MS.gene049156:CDS
GTGCTGTTACGGTGTTCTGT+TGG	0.539536	4.4:-2361109	None:intergenic
TATGGTGAATATATGAATGT+TGG	0.547151	4.4:+2361356	MS.gene049156:CDS
GAAAATTTAACAGCCTCAGA+AGG	0.553132	4.4:-2361437	None:intergenic
CAGAACACCGTAACAGCACA+AGG	0.556039	4.4:+2361113	MS.gene049156:CDS
GATTCTTGGATTTCAGGGAG+TGG	0.557003	4.4:+2361482	MS.gene049156:CDS
CAGAGTGATCGGCGCCGGAA+CGG	0.565710	4.4:-2360920	None:intergenic
GAAAACAGAGTGATCGGCGC+CGG	0.568686	4.4:-2360925	None:intergenic
GGGTCCTGCCGTATTCTCAA+AGG	0.569689	4.4:-2360874	None:intergenic
GTCGCGGTAGAATTGGCGGT+TGG	0.570719	4.4:-2360994	None:intergenic
CGGAACGGAGAGCTACTGCT+TGG	0.579824	4.4:-2360905	None:intergenic
AATGCAAAACCACCCATCCA+TGG	0.580980	4.4:-2361320	None:intergenic
TTCCGTAGATGTTGCAGTCG+CGG	0.586032	4.4:-2361010	None:intergenic
GGTGAATATATGAATGTTGG+TGG	0.591996	4.4:+2361359	MS.gene049156:CDS
ATGACCTTTGAGAATACGGC+AGG	0.599978	4.4:+2360870	MS.gene049156:CDS
ACGGTAGAAAACAGAGTGAT+CGG	0.606991	4.4:-2360931	None:intergenic
GAATATATGAATGTTGGTGG+TGG	0.621736	4.4:+2361362	MS.gene049156:CDS
CGGTTTCAAATCATTTGCTG+CGG	0.623680	4.4:-2361189	None:intergenic
ACAGATCCAAATGAAAACAC+AGG	0.641536	4.4:+2361140	MS.gene049156:CDS
AAACATATCTTGGTAGACCA+TGG	0.646826	4.4:+2361228	MS.gene049156:CDS
GGTGGTGGTGCTAATACTGG+TGG	0.661832	4.4:+2361377	MS.gene049156:CDS
ATGGCAGAAATATTCAAGAA+CGG	0.671890	4.4:+2361247	MS.gene049156:CDS
TTAGTAATAACATGAAAACC+AGG	0.674041	4.4:-2361413	None:intergenic
GGTAGCCTTTGAAGGCACAA+CGG	0.688392	4.4:-2360950	None:intergenic
GATGGTTTGATAAATTCACA+AGG	0.696704	4.4:+2361290	MS.gene049156:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTCAGTTAAAACATATCT+TGG	+	chr4.4:2361218-2361237	MS.gene049156:CDS	20.0%
!!	GTAATGAAAAGTAATATTGA+TGG	+	chr4.4:2361272-2361291	MS.gene049156:CDS	20.0%
!!!	ATTTTCTGTTGGTAATTTTT+TGG	+	chr4.4:2361454-2361473	MS.gene049156:CDS	20.0%
!	TATGGTGAATATATGAATGT+TGG	+	chr4.4:2361356-2361375	MS.gene049156:CDS	25.0%
!	TTAGTAATAACATGAAAACC+AGG	-	chr4.4:2361416-2361435	None:intergenic	25.0%
!	TTTGATAAATTCACAAGGTT+GGG	+	chr4.4:2361295-2361314	MS.gene049156:CDS	25.0%
	ATGGCAGAAATATTCAAGAA+CGG	+	chr4.4:2361247-2361266	MS.gene049156:CDS	30.0%
	GATGGTTTGATAAATTCACA+AGG	+	chr4.4:2361290-2361309	MS.gene049156:CDS	30.0%
	GCATTGAGTACACTTTATTA+TGG	+	chr4.4:2361338-2361357	MS.gene049156:CDS	30.0%
	GTTTGATAAATTCACAAGGT+TGG	+	chr4.4:2361294-2361313	MS.gene049156:CDS	30.0%
	TGTTATTACTAATCCTTCTG+AGG	+	chr4.4:2361424-2361443	MS.gene049156:CDS	30.0%
!	ACAATTCCTGTGTTTTCATT+TGG	-	chr4.4:2361149-2361168	None:intergenic	30.0%
!!	GGAACTATTGATTTCATCTT+CGG	+	chr4.4:2361029-2361048	MS.gene049156:CDS	30.0%
!!!	GTTTTAACTGAATTCTGAAC+CGG	-	chr4.4:2361212-2361231	None:intergenic	30.0%
	AAACATATCTTGGTAGACCA+TGG	+	chr4.4:2361228-2361247	MS.gene049156:CDS	35.0%
	ACAGATCCAAATGAAAACAC+AGG	+	chr4.4:2361140-2361159	MS.gene049156:CDS	35.0%
	ATACTGGTGGAAGAGTTAAT+TGG	+	chr4.4:2361390-2361409	MS.gene049156:CDS	35.0%
	GAAAATTTAACAGCCTCAGA+AGG	-	chr4.4:2361440-2361459	None:intergenic	35.0%
	GGTGAATATATGAATGTTGG+TGG	+	chr4.4:2361359-2361378	MS.gene049156:CDS	35.0%
!	AATCATTTGCTGCGGTTATA+CGG	-	chr4.4:2361184-2361203	None:intergenic	35.0%
!	GAATATATGAATGTTGGTGG+TGG	+	chr4.4:2361362-2361381	MS.gene049156:CDS	35.0%
!	GAGGCTGTTAAATTTTCTGT+TGG	+	chr4.4:2361443-2361462	MS.gene049156:CDS	35.0%
!	GTTCTTGAATATTTCTGCCA+TGG	-	chr4.4:2361248-2361267	None:intergenic	35.0%
!!!	ATTTTTTGGCTGCTGATTCT+TGG	+	chr4.4:2361468-2361487	MS.gene049156:CDS	35.0%
	ACGGTAGAAAACAGAGTGAT+CGG	-	chr4.4:2360934-2360953	None:intergenic	40.0%
!	CGCAGCAAATGATTTGAAAC+CGG	+	chr4.4:2361190-2361209	MS.gene049156:CDS	40.0%
!	CGGTTTCAAATCATTTGCTG+CGG	-	chr4.4:2361192-2361211	None:intergenic	40.0%
!	CGTAAGCATATAAGGTGTCT+TGG	-	chr4.4:2360974-2360993	None:intergenic	40.0%
!	GGACATGACCTTTGAGAATA+CGG	+	chr4.4:2360866-2360885	MS.gene049156:CDS	40.0%
!	TGTTCTTCCTTGTGCTGTTA+CGG	-	chr4.4:2361123-2361142	None:intergenic	40.0%
!!	CGAAGATGTTGCAGTTTTGA+AGG	-	chr4.4:2361070-2361089	None:intergenic	40.0%
!!	CTGCTGATTCTTGGATTTCA+GGG	+	chr4.4:2361477-2361496	MS.gene049156:CDS	40.0%
!!!	AGCTACTGCTTGGTGTTTTT+GGG	-	chr4.4:2360898-2360917	None:intergenic	40.0%
	AATGCAAAACCACCCATCCA+TGG	-	chr4.4:2361323-2361342	None:intergenic	45.0%
	GATTCTTGGATTTCAGGGAG+TGG	+	chr4.4:2361482-2361501	MS.gene049156:CDS	45.0%
	GGGAGATGGTTTCATAGCTA+AGG	+	chr4.4:2360845-2360864	MS.gene049156:CDS	45.0%
	TTCTACCGTTGTGCCTTCAA+AGG	+	chr4.4:2360945-2360964	MS.gene049156:CDS	45.0%
!	ATGACCTTTGAGAATACGGC+AGG	+	chr4.4:2360870-2360889	MS.gene049156:CDS	45.0%
!	GTGTTCTGTTGGTTGCTCAT+TGG	-	chr4.4:2361101-2361120	None:intergenic	45.0%
!!	GCTGCTGATTCTTGGATTTC+AGG	+	chr4.4:2361476-2361495	MS.gene049156:CDS	45.0%
!!!	GAGCTACTGCTTGGTGTTTT+TGG	-	chr4.4:2360899-2360918	None:intergenic	45.0%
!!!	GCTACTGCTTGGTGTTTTTG+GGG	-	chr4.4:2360897-2360916	None:intergenic	45.0%
	ATTCACAAGGTTGGGCTCCA+TGG	+	chr4.4:2361303-2361322	MS.gene049156:CDS	50.0%
	CAGAACACCGTAACAGCACA+AGG	+	chr4.4:2361113-2361132	MS.gene049156:CDS	50.0%
	GCGGTTGGCGTAAGCATATA+AGG	-	chr4.4:2360982-2361001	None:intergenic	50.0%
	GGTGGAAGAGTTAATTGGCC+TGG	+	chr4.4:2361395-2361414	MS.gene049156:CDS	50.0%
	TACCGCGACTGCAACATCTA+CGG	+	chr4.4:2361008-2361027	MS.gene049156:CDS	50.0%
	TGTTGCAGTCGCGGTAGAAT+TGG	-	chr4.4:2361004-2361023	None:intergenic	50.0%
	TTCCGTAGATGTTGCAGTCG+CGG	-	chr4.4:2361013-2361032	None:intergenic	50.0%
!	GGTAGCCTTTGAAGGCACAA+CGG	-	chr4.4:2360953-2360972	None:intergenic	50.0%
!	GGTGTCTTGGTAGCCTTTGA+AGG	-	chr4.4:2360961-2360980	None:intergenic	50.0%
!	GTGCTGTTACGGTGTTCTGT+TGG	-	chr4.4:2361112-2361131	None:intergenic	50.0%
!!	GTTGGTGGTGGTGCTAATAC+TGG	+	chr4.4:2361374-2361393	MS.gene049156:CDS	50.0%
	ACAAGGTTGGGCTCCATGGA+TGG	+	chr4.4:2361307-2361326	MS.gene049156:CDS	55.0%
	CAAGCAGTAGCTCTCCGTTC+CGG	+	chr4.4:2360906-2360925	MS.gene049156:CDS	55.0%
	CAAGGTTGGGCTCCATGGAT+GGG	+	chr4.4:2361308-2361327	MS.gene049156:CDS	55.0%
	GAAAACAGAGTGATCGGCGC+CGG	-	chr4.4:2360928-2360947	None:intergenic	55.0%
	GGGTCCTGCCGTATTCTCAA+AGG	-	chr4.4:2360877-2360896	None:intergenic	55.0%
	TGCAGTCGCGGTAGAATTGG+CGG	-	chr4.4:2361001-2361020	None:intergenic	55.0%
!	GGTGGTGGTGCTAATACTGG+TGG	+	chr4.4:2361377-2361396	MS.gene049156:CDS	55.0%
!!	AGTGGTGTGCCGTTTGATGC+AGG	+	chr4.4:2361500-2361519	MS.gene049156:CDS	55.0%
	CGGAACGGAGAGCTACTGCT+TGG	-	chr4.4:2360908-2360927	None:intergenic	60.0%
	GTCGCGGTAGAATTGGCGGT+TGG	-	chr4.4:2360997-2361016	None:intergenic	60.0%
	GGTTGGGCTCCATGGATGGG+TGG	+	chr4.4:2361311-2361330	MS.gene049156:CDS	65.0%
!	CAGAGTGATCGGCGCCGGAA+CGG	-	chr4.4:2360923-2360942	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	2360838	2361529	2360838	ID=MS.gene049156
chr4.4	mRNA	2360838	2361529	2360838	ID=MS.gene049156.t1;Parent=MS.gene049156
chr4.4	exon	2360838	2361529	2360838	ID=MS.gene049156.t1.exon1;Parent=MS.gene049156.t1
chr4.4	CDS	2360838	2361529	2360838	ID=cds.MS.gene049156.t1;Parent=MS.gene049156.t1

Gene Sequence

>MS.gene049156

CTGTTATGGGAGATGGTTTCATAGCTAAGGACATGACCTTTGAGAATACGGCAGGACCCCAAAAACACCAAGCAGTAGCTCTCCGTTCCGGCGCCGATCACTCTGTTTTCTACCGTTGTGCCTTCAAAGGCTACCAAGACACCTTATATGCTTACGCCAACCGCCAATTCTACCGCGACTGCAACATCTACGGAACTATTGATTTCATCTTCGGCAACGCAGTTACTGTCCTTCAAAACTGCAACATCTTCGTTAGAAAACCAATGAGCAACCAACAGAACACCGTAACAGCACAAGGAAGAACAGATCCAAATGAAAACACAGGAATTGTAATACATAACTGCCGTATAACCGCAGCAAATGATTTGAAACCGGTTCAGAATTCAGTTAAAACATATCTTGGTAGACCATGGCAGAAATATTCAAGAACGGTTGTAATGAAAAGTAATATTGATGGTTTGATAAATTCACAAGGTTGGGCTCCATGGATGGGTGGTTTTGCATTGAGTACACTTTATTATGGTGAATATATGAATGTTGGTGGTGGTGCTAATACTGGTGGAAGAGTTAATTGGCCTGGTTTTCATGTTATTACTAATCCTTCTGAGGCTGTTAAATTTTCTGTTGGTAATTTTTTGGCTGCTGATTCTTGGATTTCAGGGAGTGGTGTGCCGTTTGATGCAGGTTTGTGA

Protein sequence

>MS.gene049156.t1

LLWEMVSULRTUPLRIRQDPKNTKQULSVPAPITLFSTVVPSKATKTPYMLTPTANSTATATSTELLISSSATQLLSFKTATSSLENQUATNRTPUQHKEEQIQMKTQELUYITAVUPQQMIUNRFRIQLKHILVDHGRNIQERLUUKVILMVUUIHKVGLHGWVVLHUVHFIMVNIUMLVVVLILVEELIGLVFMLLLILLRLLNFLLVIFWLLILGFQGVVCRLMQVC