AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063695

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063695.t1 MTR_8g104655 89.267 382 24 1 1 365 160 541 0.0 684
MS.gene063695.t1 MTR_8g104645 89.431 369 22 1 14 365 4 372 0.0 660
MS.gene063695.t1 MTR_8g104580 80.366 382 25 3 1 365 152 500 0.0 589
MS.gene063695.t1 MTR_0618s0020 95.175 228 11 0 138 365 1 228 3.47e-167 466
MS.gene063695.t1 MTR_4g025690 56.283 382 125 6 1 365 157 513 1.10e-147 427
MS.gene063695.t1 MTR_4g025730 54.974 382 129 7 1 365 154 509 1.11e-141 412
MS.gene063695.t1 MTR_4g025670 59.810 316 108 2 69 365 174 489 1.01e-140 409
MS.gene063695.t1 MTR_7g108905 51.989 377 157 4 2 361 183 552 5.54e-137 401
MS.gene063695.t1 MTR_3g008640 51.429 385 147 6 1 365 163 527 6.06e-134 393
MS.gene063695.t1 MTR_7g053360 55.457 339 129 3 45 365 193 527 2.96e-133 391
MS.gene063695.t1 MTR_3g033690 55.752 339 128 3 45 365 192 526 2.97e-133 391
MS.gene063695.t1 MTR_3g008720 51.042 384 150 5 1 365 163 527 1.70e-132 389
MS.gene063695.t1 MTR_7g050870 50.777 386 158 6 1 365 180 554 3.40e-132 389
MS.gene063695.t1 MTR_3g008700 55.294 340 128 4 45 365 193 527 1.59e-131 387
MS.gene063695.t1 MTR_2g044880 50.267 374 157 4 9 365 164 525 7.59e-128 377
MS.gene063695.t1 MTR_8g023310 50.394 381 160 5 8 365 215 589 3.25e-127 377
MS.gene063695.t1 MTR_8g042900 51.688 385 148 7 2 365 214 581 2.26e-126 375
MS.gene063695.t1 MTR_4g035980 47.781 383 170 4 1 365 169 539 4.15e-126 373
MS.gene063695.t1 MTR_7g050950 53.779 344 135 5 41 365 215 553 5.62e-126 373
MS.gene063695.t1 MTR_1g008140 48.454 388 167 8 2 365 229 607 2.44e-124 371
MS.gene063695.t1 MTR_3g091380 48.579 387 167 6 2 365 220 597 6.27e-124 369
MS.gene063695.t1 MTR_7g050980 52.786 341 138 4 44 365 219 555 6.59e-124 368
MS.gene063695.t1 MTR_4g087980 51.176 340 143 3 43 365 183 516 3.26e-121 360
MS.gene063695.t1 MTR_7g112580 47.917 384 162 6 2 365 177 542 6.92e-120 357
MS.gene063695.t1 MTR_7g108900 50.708 353 143 4 39 361 205 556 2.31e-119 357
MS.gene063695.t1 MTR_1g083520 50.000 378 162 7 9 365 157 528 4.59e-119 355
MS.gene063695.t1 MTR_7g108890 52.695 334 137 4 51 365 231 562 2.22e-118 354
MS.gene063695.t1 MTR_7g108875 47.837 393 175 5 2 365 173 564 3.58e-118 353
MS.gene063695.t1 MTR_3g010780 48.312 385 160 7 2 365 208 574 5.98e-118 353
MS.gene063695.t1 MTR_1g088020 52.836 335 137 4 51 365 222 555 7.91e-117 350
MS.gene063695.t1 MTR_1g054880 51.796 334 140 4 51 365 227 558 3.30e-115 346
MS.gene063695.t1 MTR_4g087970 48.824 340 151 3 43 365 184 517 5.37e-114 342
MS.gene063695.t1 MTR_8g042910 49.849 331 146 3 54 365 283 612 8.88e-114 344
MS.gene063695.t1 MTR_4g124550 46.373 386 169 7 7 365 219 593 1.80e-112 340
MS.gene063695.t1 MTR_1g088225 51.940 335 138 5 51 365 222 553 1.96e-111 336
MS.gene063695.t1 MTR_4g124520 45.812 382 168 9 4 365 159 521 6.76e-110 331
MS.gene063695.t1 MTR_1g086210 47.059 357 156 5 38 365 217 569 6.99e-110 333
MS.gene063695.t1 MTR_8g023150 47.656 384 168 9 1 365 213 582 1.63e-108 329
MS.gene063695.t1 MTR_2g044810 43.799 379 177 6 4 365 175 534 1.53e-107 325
MS.gene063695.t1 MTR_0458s0030 57.088 261 94 2 122 365 55 314 6.73e-107 316
MS.gene063695.t1 MTR_1g088040 57.088 261 94 2 122 365 55 314 6.73e-107 316
MS.gene063695.t1 MTR_3g010770 46.866 335 157 4 50 365 243 575 3.78e-105 320
MS.gene063695.t1 MTR_2g044660 50.000 322 138 5 61 365 317 632 2.07e-104 320
MS.gene063695.t1 MTR_1g086190 41.927 384 198 7 2 365 185 563 6.19e-100 307
MS.gene063695.t1 MTR_1g083470 42.408 382 191 7 1 361 193 566 5.65e-98 302
MS.gene063695.t1 MTR_1g083500 42.408 382 191 7 1 361 193 566 5.65e-98 302
MS.gene063695.t1 MTR_1g083490 41.623 382 194 7 1 361 198 571 1.21e-96 299
MS.gene063695.t1 MTR_4g005170 41.885 382 193 7 1 361 198 571 2.94e-96 299
MS.gene063695.t1 MTR_7g112590 41.646 401 187 8 1 364 192 582 1.19e-94 294
MS.gene063695.t1 MTR_4g120290 44.125 383 182 9 7 364 196 571 1.42e-94 293
MS.gene063695.t1 MTR_4g120280 44.416 385 178 10 7 364 196 571 1.64e-94 293
MS.gene063695.t1 MTR_1g083510 39.655 406 191 9 1 364 189 582 4.46e-93 290
MS.gene063695.t1 MTR_4g005180 40.838 382 197 7 1 361 180 553 9.44e-93 288
MS.gene063695.t1 MTR_7g110490 42.051 390 193 6 2 360 182 569 2.19e-92 288
MS.gene063695.t1 MTR_7g110510 42.051 390 193 6 2 360 182 569 2.19e-92 288
MS.gene063695.t1 MTR_2g436040 41.926 353 170 8 35 361 199 542 1.01e-88 279
MS.gene063695.t1 MTR_2g044810 52.609 230 108 1 136 365 237 465 2.05e-88 274
MS.gene063695.t1 MTR_7g110440 40.302 397 200 7 2 365 182 574 6.93e-88 276
MS.gene063695.t1 MTR_7g110560 37.958 382 205 6 2 365 182 549 2.71e-86 271
MS.gene063695.t1 MTR_8g093460 43.614 321 148 7 68 359 229 545 3.82e-85 268
MS.gene063695.t1 MTR_3g095920 38.308 402 191 9 3 358 167 557 1.88e-81 259
MS.gene063695.t1 MTR_8g088610 38.279 337 181 4 44 361 44 372 2.25e-81 253
MS.gene063695.t1 MTR_5g083620 40.058 342 181 7 43 365 20 356 3.63e-78 244
MS.gene063695.t1 MTR_2g436050 41.143 350 176 6 38 364 221 563 4.29e-78 250
MS.gene063695.t1 MTR_7g107180 40.129 309 169 5 68 365 4 307 1.01e-77 241
MS.gene063695.t1 MTR_2g105560 39.344 305 171 5 70 365 40 339 1.94e-77 242
MS.gene063695.t1 MTR_7g110420 41.499 347 164 7 38 348 217 560 3.48e-74 241
MS.gene063695.t1 MTR_7g110430 37.119 361 157 7 38 365 217 540 1.64e-65 217
MS.gene063695.t1 MTR_7g111560 34.985 323 176 7 71 365 240 556 4.04e-58 197
MS.gene063695.t1 MTR_0458s0010 38.507 335 148 8 51 365 222 518 3.01e-57 194
MS.gene063695.t1 MTR_2g039530 38.636 308 150 8 44 331 30 318 1.42e-53 180
MS.gene063695.t1 MTR_5g083590 32.698 315 143 6 69 365 5 268 1.12e-51 173
MS.gene063695.t1 MTR_6g024165 34.295 312 159 9 71 354 41 334 2.04e-49 169
MS.gene063695.t1 MTR_6g088810 35.197 304 153 7 79 354 90 377 1.10e-47 166
MS.gene063695.t1 MTR_6g012800 31.148 305 168 5 71 348 6 295 5.18e-46 160
MS.gene063695.t1 MTR_8g102380 34.185 313 160 7 72 354 81 377 7.85e-46 161
MS.gene063695.t1 MTR_6g042660 33.451 284 156 6 62 327 28 296 1.32e-45 159
MS.gene063695.t1 MTR_7g011530 30.864 324 176 7 73 363 8 316 6.44e-44 154
MS.gene063695.t1 MTR_1g086540 30.097 309 173 6 73 354 52 344 2.98e-43 153
MS.gene063695.t1 MTR_7g092780 29.581 382 218 9 1 355 31 388 1.87e-41 149
MS.gene063695.t1 MTR_6g042690 33.333 291 152 8 62 327 30 303 1.20e-40 146
MS.gene063695.t1 MTR_7g102600 26.933 401 228 11 2 354 10 393 1.35e-38 142
MS.gene063695.t1 MTR_1g033050 29.000 300 174 6 79 358 26 306 4.96e-38 139
MS.gene063695.t1 MTR_8g102380 34.241 257 126 5 72 298 81 324 5.24e-38 139
MS.gene063695.t1 MTR_5g088280 31.438 299 173 7 71 355 39 319 2.58e-36 134
MS.gene063695.t1 MTR_5g088290 31.000 300 174 7 71 355 48 329 2.27e-35 132
MS.gene063695.t1 MTR_5g088300 31.438 299 173 7 71 355 37 317 4.83e-35 131
MS.gene063695.t1 MTR_4g124740 29.677 310 168 11 71 354 54 339 1.08e-34 130
MS.gene063695.t1 MTR_5g093970 30.132 302 180 7 71 355 43 330 4.57e-34 129
MS.gene063695.t1 MTR_5g095490 29.642 307 171 6 77 354 67 357 1.20e-33 128
MS.gene063695.t1 MTR_4g051810 28.981 314 192 7 56 355 22 318 1.84e-32 124
MS.gene063695.t1 MTR_5g012600 28.571 329 184 9 66 363 56 364 1.23e-30 120
MS.gene063695.t1 MTR_4g074290 28.623 276 163 6 71 324 71 334 4.10e-30 119
MS.gene063695.t1 MTR_2g033970 27.492 331 205 7 46 354 29 346 4.31e-30 118
MS.gene063695.t1 MTR_8g036225 27.832 309 193 8 65 355 27 323 1.08e-29 117
MS.gene063695.t1 MTR_2g073390 28.315 279 164 7 71 326 71 336 8.98e-29 115
MS.gene063695.t1 MTR_0009s0350 28.520 277 162 7 71 324 71 334 2.24e-28 114
MS.gene063695.t1 MTR_0009s0340 28.520 277 162 7 71 324 71 334 2.24e-28 114
MS.gene063695.t1 MTR_2g436600 26.807 332 197 10 46 354 28 336 1.15e-25 106
MS.gene063695.t1 MTR_6g025590 30.481 187 99 5 53 214 36 216 1.61e-20 89.7
MS.gene063695.t1 MTR_0408s0030 40.404 99 46 3 231 327 36 123 1.09e-15 73.9
MS.gene063695.t1 MTR_4g130790 28.834 163 95 4 102 243 55 217 2.96e-13 69.7
MS.gene063695.t1 MTR_2g436070 44.681 94 41 4 38 128 221 306 2.57e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063695.t1 AT1G23200 52.658 395 142 7 4 365 172 554 2.29e-139 407
MS.gene063695.t1 AT2G45220 58.235 340 113 6 43 365 184 511 5.86e-139 405
MS.gene063695.t1 AT3G60730 56.563 320 120 2 65 365 200 519 3.04e-132 388
MS.gene063695.t1 AT5G53370 48.953 382 171 6 2 365 210 585 8.17e-126 374
MS.gene063695.t1 AT5G53370 48.953 382 171 6 2 365 246 621 1.75e-125 374
MS.gene063695.t1 AT3G49220 49.354 387 164 8 2 365 219 596 7.65e-125 372
MS.gene063695.t1 AT1G11580 51.884 345 147 3 38 365 215 557 2.40e-124 369
MS.gene063695.t1 AT3G49220 49.354 387 164 8 2 365 264 641 2.40e-124 372
MS.gene063695.t1 AT1G11580 51.884 345 147 3 38 365 236 578 7.19e-124 369
MS.gene063695.t1 AT3G14310 52.738 347 141 3 36 365 252 592 6.59e-123 367
MS.gene063695.t1 AT1G53830 48.705 386 164 5 8 365 208 587 7.00e-123 367
MS.gene063695.t1 AT5G51490 45.953 383 170 5 3 365 171 536 5.10e-121 360
MS.gene063695.t1 AT3G59010 50.789 380 149 8 4 365 169 528 1.03e-120 359
MS.gene063695.t1 AT5G51500 46.997 383 168 5 3 365 173 540 2.00e-120 358
MS.gene063695.t1 AT4G02330 53.571 336 135 5 50 365 237 571 1.44e-119 357
MS.gene063695.t1 AT3G47400 46.982 381 171 4 4 365 226 594 4.07e-119 357
MS.gene063695.t1 AT4G02320 51.479 338 143 4 45 365 185 518 5.75e-119 354
MS.gene063695.t1 AT3G43270 51.917 339 141 3 44 365 192 525 9.31e-118 351
MS.gene063695.t1 AT5G27870 48.986 345 154 5 38 365 223 562 3.42e-117 356
MS.gene063695.t1 AT4G02300 47.326 374 166 5 9 365 173 532 3.67e-117 350
MS.gene063695.t1 AT3G05620 47.244 381 161 6 2 365 184 541 1.32e-116 349
MS.gene063695.t1 AT2G26450 47.340 376 168 7 8 365 248 611 5.48e-116 350
MS.gene063695.t1 AT1G02810 51.735 317 132 4 69 365 262 577 4.19e-113 341
MS.gene063695.t1 AT2G26440 45.478 387 182 6 1 365 168 547 4.48e-113 340
MS.gene063695.t1 AT2G43050 46.194 381 161 5 4 365 161 516 6.51e-113 338
MS.gene063695.t1 AT4G33220 46.608 339 158 3 44 365 70 402 1.33e-112 334
MS.gene063695.t1 AT3G05610 46.821 346 162 5 38 365 224 565 3.50e-112 342
MS.gene063695.t1 AT5G49180 45.889 377 176 7 7 361 196 566 1.38e-111 337
MS.gene063695.t1 AT4G33220 46.608 339 158 3 44 365 191 523 1.60e-111 335
MS.gene063695.t1 AT4G00190 51.887 318 132 4 66 365 160 474 1.12e-109 329
MS.gene063695.t1 AT2G47550 50.755 331 142 5 54 365 230 558 1.99e-109 331
MS.gene063695.t1 AT3G10710 46.194 381 171 6 2 365 198 561 4.75e-109 330
MS.gene063695.t1 AT5G04960 44.737 380 181 6 2 363 194 562 3.93e-108 328
MS.gene063695.t1 AT5G20860 44.792 384 167 9 2 365 180 538 4.14e-108 327
MS.gene063695.t1 AT3G06830 43.684 380 188 7 7 363 190 566 6.62e-108 327
MS.gene063695.t1 AT4G33230 45.263 380 179 8 8 365 234 606 3.10e-107 327
MS.gene063695.t1 AT3G10720 47.917 336 153 4 51 365 281 615 1.96e-105 323
MS.gene063695.t1 AT5G04970 49.682 314 138 3 71 365 308 620 8.78e-103 316
MS.gene063695.t1 AT4G15980 45.797 345 164 5 39 363 359 700 6.77e-99 308
MS.gene063695.t1 AT3G10720 53.462 260 103 2 123 365 1 259 9.26e-99 293
MS.gene063695.t1 AT4G15980 45.797 345 164 5 39 363 369 710 1.03e-98 308
MS.gene063695.t1 AT3G27980 45.697 337 158 4 46 365 169 497 1.15e-97 299
MS.gene063695.t1 AT4G03930 45.697 337 162 4 46 365 192 524 1.51e-97 300
MS.gene063695.t1 AT1G11590 45.401 337 163 4 46 365 192 524 4.25e-97 298
MS.gene063695.t1 AT1G53840 41.775 383 179 9 2 359 218 581 1.38e-94 294
MS.gene063695.t1 AT3G14300 42.932 382 179 9 2 360 601 966 6.71e-94 301
MS.gene063695.t1 AT5G20860 43.353 346 157 7 2 344 180 489 2.87e-89 278
MS.gene063695.t1 AT5G09760 38.579 394 197 7 4 358 164 551 2.71e-85 269
MS.gene063695.t1 AT5G09760 38.579 394 197 7 4 358 190 577 7.98e-85 268
MS.gene063695.t1 AT2G47030 38.847 399 202 7 2 365 197 588 4.37e-81 259
MS.gene063695.t1 AT2G47040 40.318 377 186 8 21 365 226 595 1.78e-80 257
MS.gene063695.t1 AT3G62170 38.164 414 201 9 2 365 180 588 4.74e-80 256
MS.gene063695.t1 AT1G11370 46.512 258 120 2 57 297 32 288 5.18e-79 244
MS.gene063695.t1 AT5G64640 40.785 331 156 7 58 355 276 599 8.17e-79 253
MS.gene063695.t1 AT5G19730 34.700 317 162 8 67 354 81 381 2.20e-48 168
MS.gene063695.t1 AT3G29090 32.746 284 152 4 71 327 7 278 2.68e-45 158
MS.gene063695.t1 AT1G05310 31.915 329 178 8 61 359 81 393 4.24e-42 151
MS.gene063695.t1 AT2G36700 30.263 304 176 6 73 354 44 333 1.10e-40 146
MS.gene063695.t1 AT2G36710 30.671 313 180 6 71 359 91 390 3.08e-40 147
MS.gene063695.t1 AT2G21610 31.208 298 175 8 71 354 51 332 2.59e-39 143
MS.gene063695.t1 AT2G21610 30.872 298 176 8 71 354 56 337 3.73e-39 143
MS.gene063695.t1 AT3G24130 30.496 282 162 6 70 327 33 304 1.26e-36 135
MS.gene063695.t1 AT1G44980 39.614 207 106 3 29 218 37 241 2.07e-36 132
MS.gene063695.t1 AT5G18990 31.081 296 163 7 56 327 21 299 1.08e-35 133
MS.gene063695.t1 AT3G17060 28.013 307 182 7 71 354 46 336 1.52e-35 133
MS.gene063695.t1 AT5G47500 28.783 337 193 8 50 355 38 358 1.89e-34 130
MS.gene063695.t1 AT5G07430 28.947 304 181 6 71 354 67 355 1.90e-34 130
MS.gene063695.t1 AT5G55590 32.166 314 166 7 71 354 82 378 5.66e-32 124
MS.gene063695.t1 AT5G61680 27.297 381 227 13 1 354 1 358 1.03e-31 123
MS.gene063695.t1 AT5G26810 28.302 318 178 9 71 355 34 334 1.76e-31 122
MS.gene063695.t1 AT2G19150 28.221 326 190 9 62 361 32 339 3.79e-31 121
MS.gene063695.t1 AT5G55590 31.907 257 133 5 71 298 82 325 8.54e-30 117
MS.gene063695.t1 AT5G07420 27.922 308 179 10 71 354 67 355 1.45e-29 117
MS.gene063695.t1 AT2G47280 27.036 307 189 7 71 355 35 328 7.20e-27 109
MS.gene063695.t1 AT2G19150 29.119 261 148 7 62 298 32 279 2.33e-26 107
MS.gene063695.t1 AT1G69940 25.253 297 186 7 79 354 74 355 5.55e-26 107
MS.gene063695.t1 AT5G07410 24.916 297 187 7 79 354 74 355 9.49e-25 103
MS.gene063695.t1 AT3G49220 37.143 140 73 7 2 135 264 394 2.56e-15 77.4

Find 98 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGGTGATTCTTGGATTTC+AGG 0.138272 4.3:+1673899 MS.gene063695:CDS
GTGACAAATTTAATGATATT+TGG 0.156463 4.3:+1671884 MS.gene063695:CDS
GCATTGAGTACACTTTATTA+TGG 0.267204 4.3:+1673761 MS.gene063695:CDS
AATCATTTGCTGCGGTTATA+CGG 0.315468 4.3:-1673604 None:intergenic
CTGGTGATTCTTGGATTTCA+GGG 0.333928 4.3:+1673900 MS.gene063695:CDS
AATTTCCATACTGGCTTTCA+AGG 0.355470 4.3:+1671660 MS.gene063695:CDS
TCATCAAGTACTAAACAAAA+TGG 0.356786 4.3:+1671614 MS.gene063695:CDS
TCTGACCTTGAAAGCCAGTA+TGG 0.366153 4.3:-1671665 None:intergenic
GGAACTATTGATTTCATCTT+CGG 0.369134 4.3:+1673452 MS.gene063695:CDS
TATCAGATAAATTTCCATAC+TGG 0.372948 4.3:+1671651 MS.gene063695:CDS
TTTGATAAATTCACAAGGTT+GGG 0.373566 4.3:+1673718 MS.gene063695:CDS
CTTCAGGCAACACCGGGGTC+AGG 0.382501 4.3:+1671698 MS.gene063695:CDS
AATTCAGTTAAAACATATCT+TGG 0.398697 4.3:+1673641 MS.gene063695:CDS
TGTTGCAGTCGCGGTAGAAT+TGG 0.411073 4.3:-1673424 None:intergenic
TGTTCTTCCTTGTGCTGTTA+CGG 0.414972 4.3:-1673543 None:intergenic
ATACTGGTGGAAGAGTTAAT+TGG 0.417090 4.3:+1673813 MS.gene063695:CDS
GTAATGAAAAGTAATATTGA+TGG 0.420796 4.3:+1673695 MS.gene063695:CDS
GTTTGATAAATTCACAAGGT+TGG 0.422089 4.3:+1673717 MS.gene063695:CDS
TGAAGTTAGAGTATTGATAA+TGG 0.424970 4.3:-1671570 None:intergenic
GCGGTTGGCGTAAGCATATA+AGG 0.426322 4.3:-1673402 None:intergenic
AATAATAACTGCAGCTGTCA+TGG 0.427766 4.3:+1673247 MS.gene063695:intron
GCTGCTAAGCTTAGTGGAAA+AGG 0.429873 4.3:+1671800 MS.gene063695:CDS
CGCAGCAAATGATTTGAAAC+CGG 0.431620 4.3:+1673613 MS.gene063695:CDS
GTTGGTGGTGGTGCTAATAC+TGG 0.437055 4.3:+1673797 MS.gene063695:CDS
CAAGCAGTAGCTCTCCGTTC+CGG 0.442034 4.3:+1673329 MS.gene063695:CDS
GTCAGATAGAAAGCTTCTTC+AGG 0.443922 4.3:+1671682 MS.gene063695:CDS
GGTGTCTTGGTAGCCTTTGA+AGG 0.444283 4.3:-1673381 None:intergenic
GTGTTCTGTTGGTTGCTCAT+TGG 0.453378 4.3:-1673521 None:intergenic
GCAACACCGGGGTCAGGGAC+AGG 0.454024 4.3:+1671704 MS.gene063695:CDS
AAGAGAATGTTGATATCAAA+AGG 0.455565 4.3:+1671858 MS.gene063695:CDS
ACAAGGTTGGGCTCCATGGA+TGG 0.456078 4.3:+1673730 MS.gene063695:CDS
CAAGGTTGGGCTCCATGGAT+GGG 0.470284 4.3:+1673731 MS.gene063695:CDS
ATTCACAAGGTTGGGCTCCA+TGG 0.471108 4.3:+1673726 MS.gene063695:CDS
GGACATGACCTTTGAGAATA+CGG 0.477411 4.3:+1673289 MS.gene063695:CDS
AATGATATTTGGAGATGGAA+TGG 0.477734 4.3:+1671895 MS.gene063695:CDS
GATGCAGCTGCTAAGCTTAG+TGG 0.483722 4.3:+1671794 MS.gene063695:CDS
GTTGTTATTCATCTGAAAGC+AGG 0.486478 4.3:+1671827 MS.gene063695:CDS
TTCTACCGTTGTGCCTTCAA+AGG 0.490213 4.3:+1673368 MS.gene063695:CDS
CTGCTAAGCTTAGTGGAAAA+GGG 0.504769 4.3:+1671801 MS.gene063695:CDS
AGTGGTGTGCCGTTTGATGC+AGG 0.508356 4.3:+1673923 MS.gene063695:CDS
AAGCTTCTTCAGGCAACACC+GGG 0.510144 4.3:+1671692 MS.gene063695:CDS
TGCAGTCGCGGTAGAATTGG+CGG 0.511251 4.3:-1673421 None:intergenic
GTTCTTGAATATTTCTGCCA+TGG 0.518439 4.3:-1673668 None:intergenic
GGTGGAAGAGTTAATTGGCC+TGG 0.520232 4.3:+1673818 MS.gene063695:CDS
TAAGACAATATCTGAGGGTG+TGG 0.520252 4.3:+1671772 MS.gene063695:CDS
GAAGGCACAACGGTAGAAAA+CGG 0.523739 4.3:-1673363 None:intergenic
CTTGGTCCTGTCCCTGACCC+CGG 0.525603 4.3:-1671710 None:intergenic
ATTCACAAACCTGCATCAAA+CGG 0.526344 4.3:-1673932 None:intergenic
TTTAGTGAAGTTGCTTAACA+TGG 0.527598 4.3:-1671528 None:intergenic
TACCGCGACTGCAACATCTA+CGG 0.528232 4.3:+1673431 MS.gene063695:CDS
ACTGCAGCTGTCATGGGAGA+TGG 0.533273 4.3:+1673254 MS.gene063695:intron
CGTAAGCATATAAGGTGTCT+TGG 0.533953 4.3:-1673394 None:intergenic
GGTTGGGCTCCATGGATGGG+TGG 0.536463 4.3:+1673734 MS.gene063695:CDS
TGTTATTACTAATCCTTCTG+AGG 0.537907 4.3:+1673847 MS.gene063695:CDS
GTGCTGTTACGGTGTTCTGT+TGG 0.539536 4.3:-1673532 None:intergenic
AATTTAATGATATTTGGAGA+TGG 0.540497 4.3:+1671890 MS.gene063695:CDS
ATAATAACTGCAGCTGTCAT+GGG 0.542595 4.3:+1673248 MS.gene063695:intron
TATGGTGAATATATGAATGT+TGG 0.547151 4.3:+1673779 MS.gene063695:CDS
AAAGCTTCTTCAGGCAACAC+CGG 0.552192 4.3:+1671691 MS.gene063695:CDS
GAAAATTTAACAGCCTCAGA+AGG 0.553132 4.3:-1673860 None:intergenic
GTGGTTGCACAAGATGGAAG+TGG 0.556036 4.3:+1671743 MS.gene063695:CDS
CAGAACACCGTAACAGCACA+AGG 0.556039 4.3:+1673536 MS.gene063695:CDS
GATTCTTGGATTTCAGGGAG+TGG 0.559486 4.3:+1673905 MS.gene063695:CDS
CGGAGTGATCGGCGCCGGAA+CGG 0.559791 4.3:-1673343 None:intergenic
GGGAGATGGTTTCATAGCTA+AGG 0.562094 4.3:+1673268 MS.gene063695:CDS
TTGTTTAGTACTTGATGATG+TGG 0.565122 4.3:-1671609 None:intergenic
ACGGTAGAAAACGGAGTGAT+CGG 0.568769 4.3:-1673354 None:intergenic
GGGTCCTGCCGTATTCTCAA+AGG 0.569689 4.3:-1673297 None:intergenic
GTCGCGGTAGAATTGGCGGT+TGG 0.570719 4.3:-1673417 None:intergenic
ATGGATTCTACTATTGTCAC+GGG 0.574508 4.3:+1671914 MS.gene063695:CDS
CGGAACGGAGAGCTACTGCT+TGG 0.579824 4.3:-1673328 None:intergenic
AATGCAAAACCACCCATCCA+TGG 0.580980 4.3:-1673743 None:intergenic
GAAAACGGAGTGATCGGCGC+CGG 0.581382 4.3:-1673348 None:intergenic
TTCCGTAGATGTTGCAGTCG+CGG 0.586032 4.3:-1673433 None:intergenic
TCAAGTACTAAACAAAATGG+AGG 0.588650 4.3:+1671617 MS.gene063695:CDS
AATGGATTCTACTATTGTCA+CGG 0.588947 4.3:+1671913 MS.gene063695:CDS
GGTGAATATATGAATGTTGG+TGG 0.591996 4.3:+1673782 MS.gene063695:CDS
GATATCGTGGTTGCACAAGA+TGG 0.592172 4.3:+1671737 MS.gene063695:CDS
ATGACCTTTGAGAATACGGC+AGG 0.599978 4.3:+1673293 MS.gene063695:CDS
TTCAGGCAACACCGGGGTCA+GGG 0.601142 4.3:+1671699 MS.gene063695:CDS
GAACTATAAGACAATATCTG+AGG 0.605352 4.3:+1671766 MS.gene063695:CDS
AACTATAAGACAATATCTGA+GGG 0.612206 4.3:+1671767 MS.gene063695:CDS
CTAAACAAAATGGAGGGAGA+AGG 0.620123 4.3:+1671624 MS.gene063695:CDS
GAATATATGAATGTTGGTGG+TGG 0.621736 4.3:+1673785 MS.gene063695:CDS
CGGTTTCAAATCATTTGCTG+CGG 0.623680 4.3:-1673612 None:intergenic
CAAGTACTAAACAAAATGGA+GGG 0.628274 4.3:+1671618 MS.gene063695:CDS
AGGACCAAGTGCTGATATCG+TGG 0.631260 4.3:+1671724 MS.gene063695:CDS
GATGATGTGGATGAAGATGA+TGG 0.634150 4.3:-1671596 None:intergenic
ACAGATCCAAATGAAAACAC+AGG 0.641536 4.3:+1673563 MS.gene063695:CDS
TGGTTGCACAAGATGGAAGT+GGG 0.642324 4.3:+1671744 MS.gene063695:CDS
AAACATATCTTGGTAGACCA+TGG 0.646826 4.3:+1673651 MS.gene063695:CDS
GCAACCACGATATCAGCACT+TGG 0.658862 4.3:-1671728 None:intergenic
GGTGGTGGTGCTAATACTGG+TGG 0.661832 4.3:+1673800 MS.gene063695:CDS
ATGGCAGAAATATTCAAGAA+CGG 0.671890 4.3:+1673670 MS.gene063695:CDS
TTAGTAATAACATGAAAACC+AGG 0.674041 4.3:-1673836 None:intergenic
GGTAGCCTTTGAAGGCACAA+CGG 0.688392 4.3:-1673373 None:intergenic
GATGGTTTGATAAATTCACA+AGG 0.696704 4.3:+1673713 MS.gene063695:CDS
AGCTTCTTCAGGCAACACCG+GGG 0.762405 4.3:+1671693 MS.gene063695:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACACTATAAATATTCATTT+TGG + chr4.3:1672525-1672544 MS.gene063695:intron 15.0%
!!! TTAAAATTAGTCATCTTTTT+AGG + chr4.3:1672230-1672249 MS.gene063695:intron 15.0%
!! AATTCAGTTAAAACATATCT+TGG + chr4.3:1673641-1673660 MS.gene063695:CDS 20.0%
!! AATTTAATGATATTTGGAGA+TGG + chr4.3:1671890-1671909 MS.gene063695:CDS 20.0%
!! GAATAGGATAATTAACAAAT+AGG + chr4.3:1672692-1672711 MS.gene063695:intron 20.0%
!! GGAAAAAACGAAAAATTTAA+AGG + chr4.3:1672387-1672406 MS.gene063695:intron 20.0%
!! GTAATGAAAAGTAATATTGA+TGG + chr4.3:1673695-1673714 MS.gene063695:CDS 20.0%
!! GTGACAAATTTAATGATATT+TGG + chr4.3:1671884-1671903 MS.gene063695:CDS 20.0%
!! TTATTTCATAGTTAGAACTT+TGG - chr4.3:1673228-1673247 None:intergenic 20.0%
!! TTGAAACTTAAAAATCACTT+TGG + chr4.3:1672366-1672385 MS.gene063695:intron 20.0%
!!! AAAAGATGACTAATTTTAAG+TGG - chr4.3:1672229-1672248 None:intergenic 20.0%
!!! AAAGATGACTAATTTTAAGT+GGG - chr4.3:1672228-1672247 None:intergenic 20.0%
!!! ATTTTCTGTTGGTAATTTTT+TGG + chr4.3:1673877-1673896 MS.gene063695:CDS 20.0%
! AACTATAAGACAATATCTGA+GGG + chr4.3:1671767-1671786 MS.gene063695:CDS 25.0%
! AAGAGAATGTTGATATCAAA+AGG + chr4.3:1671858-1671877 MS.gene063695:CDS 25.0%
! AGAAAAGTTATCGTTACATA+TGG - chr4.3:1673079-1673098 None:intergenic 25.0%
! AGATTATTGTGTGAGAATAT+AGG + chr4.3:1672021-1672040 MS.gene063695:intron 25.0%
! AGGAAAAACGAAAAACTTAA+AGG - chr4.3:1672065-1672084 None:intergenic 25.0%
! ATATTATTGCTGTGATTACA+CGG - chr4.3:1672957-1672976 None:intergenic 25.0%
! ATTACTTACTTTCTCACAAA+AGG + chr4.3:1672769-1672788 MS.gene063695:intron 25.0%
! GAAACGATTCAAAAACTAAA+AGG + chr4.3:1672328-1672347 MS.gene063695:intron 25.0%
! GAAACGATTCAAAAACTAAA+AGG - chr4.3:1672347-1672328 None:intergenic 25.0%
! TATCAGATAAATTTCCATAC+TGG + chr4.3:1671651-1671670 MS.gene063695:CDS 25.0%
! TATGGTGAATATATGAATGT+TGG + chr4.3:1673779-1673798 MS.gene063695:CDS 25.0%
! TCATCAAGTACTAAACAAAA+TGG + chr4.3:1671614-1671633 MS.gene063695:CDS 25.0%
! TTAGTAATAACATGAAAACC+AGG - chr4.3:1673839-1673858 None:intergenic 25.0%
! TTTGATAAATTCACAAGGTT+GGG + chr4.3:1673718-1673737 MS.gene063695:CDS 25.0%
!! AAAAACTAAAAGGACTTGTT+TGG + chr4.3:1672338-1672357 MS.gene063695:intron 25.0%
!! AAAACTAAAAGGACTTGTTT+GGG + chr4.3:1672339-1672358 MS.gene063695:intron 25.0%
!!! ATTAGTCATCTTTTTAGGTA+TGG + chr4.3:1672235-1672254 MS.gene063695:intron 25.0%
!!! TGAAGTTAGAGTATTGATAA+TGG - chr4.3:1671573-1671592 None:intergenic 25.0%
!!! TGAGACTTTTGAAACTTAAA+AGG - chr4.3:1672096-1672115 None:intergenic 25.0%
!!! TTTCAAACAAGTCATTTTAG+AGG - chr4.3:1672312-1672331 None:intergenic 25.0%
AAACTTAAAAGGACCACTTT+AGG - chr4.3:1672085-1672104 None:intergenic 30.0%
AATGATATTTGGAGATGGAA+TGG + chr4.3:1671895-1671914 MS.gene063695:CDS 30.0%
ATGGCAGAAATATTCAAGAA+CGG + chr4.3:1673670-1673689 MS.gene063695:CDS 30.0%
CAAGTACTAAACAAAATGGA+GGG + chr4.3:1671618-1671637 MS.gene063695:CDS 30.0%
GAACTATAAGACAATATCTG+AGG + chr4.3:1671766-1671785 MS.gene063695:CDS 30.0%
GATGGTTTGATAAATTCACA+AGG + chr4.3:1673713-1673732 MS.gene063695:CDS 30.0%
GCATTGAGTACACTTTATTA+TGG + chr4.3:1673761-1673780 MS.gene063695:CDS 30.0%
GGTAATCAAAATGCTATAGA+TGG + chr4.3:1671935-1671954 MS.gene063695:intron 30.0%
GTTTGATAAATTCACAAGGT+TGG + chr4.3:1673717-1673736 MS.gene063695:CDS 30.0%
TATAGGTCAAATGCATCTAA+AGG + chr4.3:1672038-1672057 MS.gene063695:intron 30.0%
TCAAGTACTAAACAAAATGG+AGG + chr4.3:1671617-1671636 MS.gene063695:CDS 30.0%
TCTCGATCAAATGAATCTAA+AGG - chr4.3:1672414-1672433 None:intergenic 30.0%
TGTACAAATTGTGTTACGTA+CGG + chr4.3:1672590-1672609 MS.gene063695:intron 30.0%
TGTTATTACTAATCCTTCTG+AGG + chr4.3:1673847-1673866 MS.gene063695:CDS 30.0%
! AATGGATTCTACTATTGTCA+CGG + chr4.3:1671913-1671932 MS.gene063695:CDS 30.0%
! ACAATTCCTGTGTTTTCATT+TGG - chr4.3:1673572-1673591 None:intergenic 30.0%
!! GATTGTTTGCTATTATGCAT+GGG + chr4.3:1672443-1672462 MS.gene063695:intron 30.0%
!! GGAACTATTGATTTCATCTT+CGG + chr4.3:1673452-1673471 MS.gene063695:CDS 30.0%
!! GTTTTTCGTTTTTCCTAAAG+TGG + chr4.3:1672069-1672088 MS.gene063695:intron 30.0%
!! TCGTTTTGCTATCAAAGAAT+AGG + chr4.3:1672676-1672695 MS.gene063695:intron 30.0%
!! TGATTGTTTGCTATTATGCA+TGG + chr4.3:1672442-1672461 MS.gene063695:intron 30.0%
!! TTGTTTAGTACTTGATGATG+TGG - chr4.3:1671612-1671631 None:intergenic 30.0%
!!! ATCTTTTTAGGTATGGCAAA+TGG + chr4.3:1672242-1672261 MS.gene063695:intron 30.0%
!!! GTTTTAACTGAATTCTGAAC+CGG - chr4.3:1673635-1673654 None:intergenic 30.0%
AAACATATCTTGGTAGACCA+TGG + chr4.3:1673651-1673670 MS.gene063695:CDS 35.0%
AATAATAACTGCAGCTGTCA+TGG + chr4.3:1673247-1673266 MS.gene063695:intron 35.0%
ACAGATCCAAATGAAAACAC+AGG + chr4.3:1673563-1673582 MS.gene063695:CDS 35.0%
ATAATAACTGCAGCTGTCAT+GGG + chr4.3:1673248-1673267 MS.gene063695:intron 35.0%
ATACTGGTGGAAGAGTTAAT+TGG + chr4.3:1673813-1673832 MS.gene063695:CDS 35.0%
ATGGATTCTACTATTGTCAC+GGG + chr4.3:1671914-1671933 MS.gene063695:CDS 35.0%
CCAACTATGCTATCTACAAT+AGG - chr4.3:1672157-1672176 None:intergenic 35.0%
CCTATTGTAGATAGCATAGT+TGG + chr4.3:1672154-1672173 MS.gene063695:intron 35.0%
GAAAATTTAACAGCCTCAGA+AGG - chr4.3:1673863-1673882 None:intergenic 35.0%
GGTGAATATATGAATGTTGG+TGG + chr4.3:1673782-1673801 MS.gene063695:CDS 35.0%
TGCATGGGATAAATCATATG+TGG + chr4.3:1672458-1672477 MS.gene063695:intron 35.0%
! AATCATTTGCTGCGGTTATA+CGG - chr4.3:1673607-1673626 None:intergenic 35.0%
! ACATTTCGTTCAGCAACTTT+TGG + chr4.3:1671965-1671984 MS.gene063695:intron 35.0%
! GAATATATGAATGTTGGTGG+TGG + chr4.3:1673785-1673804 MS.gene063695:CDS 35.0%
! GAGGCTGTTAAATTTTCTGT+TGG + chr4.3:1673866-1673885 MS.gene063695:CDS 35.0%
! GTTCTTGAATATTTCTGCCA+TGG - chr4.3:1673671-1673690 None:intergenic 35.0%
! GTTGTTATTCATCTGAAAGC+AGG + chr4.3:1671827-1671846 MS.gene063695:CDS 35.0%
!! AATATTCATTTTGGCCCCAT+TGG + chr4.3:1672534-1672553 MS.gene063695:intron 35.0%
!! AATTTCCATACTGGCTTTCA+AGG + chr4.3:1671660-1671679 MS.gene063695:CDS 35.0%
!! ATATTCATTTTGGCCCCATT+GGG + chr4.3:1672535-1672554 MS.gene063695:intron 35.0%
!!! ATTTTTTGGCTGGTGATTCT+TGG + chr4.3:1673891-1673910 MS.gene063695:CDS 35.0%
!!! TCTGTTGGTAATTTTTTGGC+TGG + chr4.3:1673881-1673900 MS.gene063695:CDS 35.0%
!!! TTTTTAGGTATGGCAAATGG+TGG + chr4.3:1672245-1672264 MS.gene063695:intron 35.0%
CTAAACAAAATGGAGGGAGA+AGG + chr4.3:1671624-1671643 MS.gene063695:CDS 40.0%
CTCATCTTTGGTACCAAATC+TGG + chr4.3:1672193-1672212 MS.gene063695:intron 40.0%
CTGCTAAGCTTAGTGGAAAA+GGG + chr4.3:1671801-1671820 MS.gene063695:CDS 40.0%
CTGGTGATTCTTGGATTTCA+GGG + chr4.3:1673900-1673919 MS.gene063695:CDS 40.0%
GATGATGTGGATGAAGATGA+TGG - chr4.3:1671599-1671618 None:intergenic 40.0%
GTCAGATAGAAAGCTTCTTC+AGG + chr4.3:1671682-1671701 MS.gene063695:CDS 40.0%
GTCTCTATCCTAATCCCAAT+GGG - chr4.3:1672552-1672571 None:intergenic 40.0%
TAAGACAATATCTGAGGGTG+TGG + chr4.3:1671772-1671791 MS.gene063695:CDS 40.0%
TACAGCAGTCTCACAAAACA+TGG + chr4.3:1673003-1673022 MS.gene063695:intron 40.0%
TCTCTATCCTAATCCCAATG+GGG - chr4.3:1672551-1672570 None:intergenic 40.0%
TGCTTCCTTGAACTCATCTT+TGG + chr4.3:1672181-1672200 MS.gene063695:intron 40.0%
TGGTACCAAAGATGAGTTCA+AGG - chr4.3:1672189-1672208 None:intergenic 40.0%
TGTCTCTATCCTAATCCCAA+TGG - chr4.3:1672553-1672572 None:intergenic 40.0%
! CGCAGCAAATGATTTGAAAC+CGG + chr4.3:1673613-1673632 MS.gene063695:CDS 40.0%
! CGGTTTCAAATCATTTGCTG+CGG - chr4.3:1673615-1673634 None:intergenic 40.0%
! CGTAAGCATATAAGGTGTCT+TGG - chr4.3:1673397-1673416 None:intergenic 40.0%
! GGACATGACCTTTGAGAATA+CGG + chr4.3:1673289-1673308 MS.gene063695:CDS 40.0%
! TGTTCTTCCTTGTGCTGTTA+CGG - chr4.3:1673546-1673565 None:intergenic 40.0%
!! CGAAGATGTTGCAGTTTTGA+AGG - chr4.3:1673493-1673512 None:intergenic 40.0%
!!! AGCTACTGCTTGGTGTTTTT+GGG - chr4.3:1673321-1673340 None:intergenic 40.0%
AAAGCTTCTTCAGGCAACAC+CGG + chr4.3:1671691-1671710 MS.gene063695:CDS 45.0%
AATGCAAAACCACCCATCCA+TGG - chr4.3:1673746-1673765 None:intergenic 45.0%
ACGGTAGAAAACGGAGTGAT+CGG - chr4.3:1673357-1673376 None:intergenic 45.0%
GATATCGTGGTTGCACAAGA+TGG + chr4.3:1671737-1671756 MS.gene063695:CDS 45.0%
GATTCTTGGATTTCAGGGAG+TGG + chr4.3:1673905-1673924 MS.gene063695:CDS 45.0%
GCTGCTAAGCTTAGTGGAAA+AGG + chr4.3:1671800-1671819 MS.gene063695:CDS 45.0%
GCTGGTGATTCTTGGATTTC+AGG + chr4.3:1673899-1673918 MS.gene063695:CDS 45.0%
GGGAGATGGTTTCATAGCTA+AGG + chr4.3:1673268-1673287 MS.gene063695:CDS 45.0%
TCTGACCTTGAAAGCCAGTA+TGG - chr4.3:1671668-1671687 None:intergenic 45.0%
TGGGTGTTAGAAGCCAGATT+TGG - chr4.3:1672209-1672228 None:intergenic 45.0%
TGGTTGCACAAGATGGAAGT+GGG + chr4.3:1671744-1671763 MS.gene063695:CDS 45.0%
TTCTACCGTTGTGCCTTCAA+AGG + chr4.3:1673368-1673387 MS.gene063695:CDS 45.0%
! ATGACCTTTGAGAATACGGC+AGG + chr4.3:1673293-1673312 MS.gene063695:CDS 45.0%
! GAAGGCACAACGGTAGAAAA+CGG - chr4.3:1673366-1673385 None:intergenic 45.0%
! GTGTTCTGTTGGTTGCTCAT+TGG - chr4.3:1673524-1673543 None:intergenic 45.0%
!! ATTTTGGCCCCATTGGGATT+AGG + chr4.3:1672541-1672560 MS.gene063695:intron 45.0%
!!! GAGCTACTGCTTGGTGTTTT+TGG - chr4.3:1673322-1673341 None:intergenic 45.0%
!!! GCTACTGCTTGGTGTTTTTG+GGG - chr4.3:1673320-1673339 None:intergenic 45.0%
AAGCTTCTTCAGGCAACACC+GGG + chr4.3:1671692-1671711 MS.gene063695:CDS 50.0%
ATTCACAAGGTTGGGCTCCA+TGG + chr4.3:1673726-1673745 MS.gene063695:CDS 50.0%
CAGAACACCGTAACAGCACA+AGG + chr4.3:1673536-1673555 MS.gene063695:CDS 50.0%
GATGCAGCTGCTAAGCTTAG+TGG + chr4.3:1671794-1671813 MS.gene063695:CDS 50.0%
GCAACCACGATATCAGCACT+TGG - chr4.3:1671731-1671750 None:intergenic 50.0%
GCGGTTGGCGTAAGCATATA+AGG - chr4.3:1673405-1673424 None:intergenic 50.0%
GGTGGAAGAGTTAATTGGCC+TGG + chr4.3:1673818-1673837 MS.gene063695:CDS 50.0%
GTGGTTGCACAAGATGGAAG+TGG + chr4.3:1671743-1671762 MS.gene063695:CDS 50.0%
TACCGCGACTGCAACATCTA+CGG + chr4.3:1673431-1673450 MS.gene063695:CDS 50.0%
TGTTGCAGTCGCGGTAGAAT+TGG - chr4.3:1673427-1673446 None:intergenic 50.0%
TTCCGTAGATGTTGCAGTCG+CGG - chr4.3:1673436-1673455 None:intergenic 50.0%
! GGTAGCCTTTGAAGGCACAA+CGG - chr4.3:1673376-1673395 None:intergenic 50.0%
! GGTGTCTTGGTAGCCTTTGA+AGG - chr4.3:1673384-1673403 None:intergenic 50.0%
! GTGCTGTTACGGTGTTCTGT+TGG - chr4.3:1673535-1673554 None:intergenic 50.0%
!! AGGACCAAGTGCTGATATCG+TGG + chr4.3:1671724-1671743 MS.gene063695:CDS 50.0%
!! GTTGGTGGTGGTGCTAATAC+TGG + chr4.3:1673797-1673816 MS.gene063695:CDS 50.0%
ACAAGGTTGGGCTCCATGGA+TGG + chr4.3:1673730-1673749 MS.gene063695:CDS 55.0%
ACTGCAGCTGTCATGGGAGA+TGG + chr4.3:1673254-1673273 MS.gene063695:intron 55.0%
AGCTTCTTCAGGCAACACCG+GGG + chr4.3:1671693-1671712 MS.gene063695:CDS 55.0%
CAAGCAGTAGCTCTCCGTTC+CGG + chr4.3:1673329-1673348 MS.gene063695:CDS 55.0%
CAAGGTTGGGCTCCATGGAT+GGG + chr4.3:1673731-1673750 MS.gene063695:CDS 55.0%
GGGTCCTGCCGTATTCTCAA+AGG - chr4.3:1673300-1673319 None:intergenic 55.0%
TGCAGTCGCGGTAGAATTGG+CGG - chr4.3:1673424-1673443 None:intergenic 55.0%
! GGTGGTGGTGCTAATACTGG+TGG + chr4.3:1673800-1673819 MS.gene063695:CDS 55.0%
!! AGTGGTGTGCCGTTTGATGC+AGG + chr4.3:1673923-1673942 MS.gene063695:CDS 55.0%
CGGAACGGAGAGCTACTGCT+TGG - chr4.3:1673331-1673350 None:intergenic 60.0%
GAAAACGGAGTGATCGGCGC+CGG - chr4.3:1673351-1673370 None:intergenic 60.0%
GTCGCGGTAGAATTGGCGGT+TGG - chr4.3:1673420-1673439 None:intergenic 60.0%
TTCAGGCAACACCGGGGTCA+GGG + chr4.3:1671699-1671718 MS.gene063695:CDS 60.0%
CTTCAGGCAACACCGGGGTC+AGG + chr4.3:1671698-1671717 MS.gene063695:CDS 65.0%
CTTGGTCCTGTCCCTGACCC+CGG - chr4.3:1671713-1671732 None:intergenic 65.0%
GGTTGGGCTCCATGGATGGG+TGG + chr4.3:1673734-1673753 MS.gene063695:CDS 65.0%
GCAACACCGGGGTCAGGGAC+AGG + chr4.3:1671704-1671723 MS.gene063695:CDS 70.0%
! CGGAGTGATCGGCGCCGGAA+CGG - chr4.3:1673346-1673365 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 1671530 1673952 1671530 ID=MS.gene063695
chr4.3 mRNA 1671530 1673952 1671530 ID=MS.gene063695.t1;Parent=MS.gene063695
chr4.3 exon 1671530 1671935 1671530 ID=MS.gene063695.t1.exon1;Parent=MS.gene063695.t1
chr4.3 CDS 1671530 1671935 1671530 ID=cds.MS.gene063695.t1;Parent=MS.gene063695.t1
chr4.3 exon 1673261 1673952 1673261 ID=MS.gene063695.t1.exon2;Parent=MS.gene063695.t1
chr4.3 CDS 1673261 1673952 1673261 ID=cds.MS.gene063695.t1;Parent=MS.gene063695.t1
Gene Sequence

>MS.gene063695

ATGTTAAGCAACTTCACTAAATTGCTTAGCAATTCTTTGTCCATTATCAATACTCTAACTTCATCGCCATCATCTTCATCCACATCATCAAGTACTAAACAAAATGGAGGGAGAAGGTTGTTATCAGATAAATTTCCATACTGGCTTTCAAGGTCAGATAGAAAGCTTCTTCAGGCAACACCGGGGTCAGGGACAGGACCAAGTGCTGATATCGTGGTTGCACAAGATGGAAGTGGGAACTATAAGACAATATCTGAGGGTGTGGATGCAGCTGCTAAGCTTAGTGGAAAAGGGAGAGTTGTTATTCATCTGAAAGCAGGTGTTTATAAAGAGAATGTTGATATCAAAAGGACAGTGACAAATTTAATGATATTTGGAGATGGAATGGATTCTACTATTGTCACGGGTAATCAAAATGCTATAGATGGTTCTACTACATTTCGTTCAGCAACTTTTGGTAAGTTCTTCAATAGTTAATTACTCATTCATATAGATTATTGTGTGAGAATATAGGTCAAATGCATCTAAAGGTCCTTTAAGTTTTTCGTTTTTCCTAAAGTGGTCCTTTTAAGTTTCAAAAGTCTCAAACAAGTCCTTTTAGTTTTTGAATCGTTTCAAACAAGTCCTATTGTAGATAGCATAGTTGGTAATTGCTTCCTTGAACTCATCTTTGGTACCAAATCTGGCTTCTAACACCCACTTAAAATTAGTCATCTTTTTAGGTATGGCAAATGGTGGATAATGCTCTTTGTTGCTATCATCTGAATCATCTTCATCATCCTCTAAAATGACTTGTTTGAAACGATTCAAAAACTAAAAGGACTTGTTTGGGACTTTTGAAACTTAAAAATCACTTTGGAAAAAACGAAAAATTTAAAGGACCTTTAGATTCATTTGATCGAGAATATATTATGATTGTTTGCTATTATGCATGGGATAAATCATATGTGGAATAGTAACAATGATAGTAATATAAAAACACTTTGAATTAACTGAACACTATAAATATTCATTTTGGCCCCATTGGGATTAGGATAGAGACATATGCAGTACTTACTTTTGTACAAATTGTGTTACGTACGGATCATACATCACTTCTTTTTTTGATAAGCATATAGCTCATATAATATTATATTGAGAAAATTGTCGTTTTGCTATCAAAGAATAGGATAATTAACAAATAGGATAATTAACAAAAAGTACAAAGACGATAATATTTTCAAAATAATCTCTTTTTTAATTACTTACTTTCTCACAAAAGGTACTTTTTGTTATATTTTTTTTTTTAGACTTGTTAATTAAAACATGTTCAAACGCATATTCAAAAAAAAAAAAAAAATTAGCTAAGATATGATCATGTCAATTATAAAACTCTAATAACTTTAATTTTTCTTTCAAAAAAAAAAAAACTCTAATTTCTATAACCGTGTAATCACAGCAATAATATATCAATATTACTCAACATACACTTTTTACAGCAGTCTCACAAAACATGGTATCTATCTATGATGTTCTTACTTTATATCATGCGAAGAAAATATCTTATCCATATGTAACGATAACTTTTCTAACAAAATACAATGCATTGTAATTGCAAATTTCTTTCACAGTAAATTAGAGTCGATCATTTGTATGACTTTAAAAGTAATTAATGATAAAAGTCTTACTAATTAACTAGATGTATATAAATTAAATCCAAAGTTCTAACTATGAAATAAATAATAACTGCAGCTGTCATGGGAGATGGTTTCATAGCTAAGGACATGACCTTTGAGAATACGGCAGGACCCCAAAAACACCAAGCAGTAGCTCTCCGTTCCGGCGCCGATCACTCCGTTTTCTACCGTTGTGCCTTCAAAGGCTACCAAGACACCTTATATGCTTACGCCAACCGCCAATTCTACCGCGACTGCAACATCTACGGAACTATTGATTTCATCTTCGGCAACGCAGTTACTGTCCTTCAAAACTGCAACATCTTCGTTAGAAAACCAATGAGCAACCAACAGAACACCGTAACAGCACAAGGAAGAACAGATCCAAATGAAAACACAGGAATTGTAATACATAACTGCCGTATAACCGCAGCAAATGATTTGAAACCGGTTCAGAATTCAGTTAAAACATATCTTGGTAGACCATGGCAGAAATATTCAAGAACGGTTGTAATGAAAAGTAATATTGATGGTTTGATAAATTCACAAGGTTGGGCTCCATGGATGGGTGGTTTTGCATTGAGTACACTTTATTATGGTGAATATATGAATGTTGGTGGTGGTGCTAATACTGGTGGAAGAGTTAATTGGCCTGGTTTTCATGTTATTACTAATCCTTCTGAGGCTGTTAAATTTTCTGTTGGTAATTTTTTGGCTGGTGATTCTTGGATTTCAGGGAGTGGTGTGCCGTTTGATGCAGGTTTGTGA
Protein sequence

>MS.gene063695.t1

MLSNFTKLLSNSLSIINTLTSSPSSSSTSSSTKQNGGRRLLSDKFPYWLSRSDRKLLQATPGSGTGPSADIVVAQDGSGNYKTISEGVDAAAKLSGKGRVVIHLKAGVYKENVDIKRTVTNLMIFGDGMDSTIVTAVMGDGFIAKDMTFENTAGPQKHQAVALRSGADHSVFYRCAFKGYQDTLYAYANRQFYRDCNIYGTIDFIFGNAVTVLQNCNIFVRKPMSNQQNTVTAQGRTDPNENTGIVIHNCRITAANDLKPVQNSVKTYLGRPWQKYSRTVVMKSNIDGLINSQGWAPWMGGFALSTLYYGEYMNVGGGANTGGRVNWPGFHVITNPSEAVKFSVGNFLAGDSWISGSGVPFDAGL