AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063696

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063696.t1 MTR_8g104655 93.542 542 33 2 1 541 1 541 0.0 1041
MS.gene063696.t1 MTR_8g104580 87.453 534 32 4 9 541 1 500 0.0 931
MS.gene063696.t1 MTR_8g104645 92.954 369 26 0 173 541 4 372 0.0 703
MS.gene063696.t1 MTR_4g025690 50.652 537 228 11 13 541 6 513 0.0 529
MS.gene063696.t1 MTR_4g025730 50.187 534 225 13 18 541 7 509 3.77e-178 512
MS.gene063696.t1 MTR_0618s0020 95.175 228 11 0 314 541 1 228 3.74e-164 465
MS.gene063696.t1 MTR_3g008640 46.887 514 243 7 36 541 36 527 1.14e-160 468
MS.gene063696.t1 MTR_4g035980 44.942 514 261 6 36 541 40 539 4.67e-160 467
MS.gene063696.t1 MTR_3g033690 47.104 518 235 10 36 541 36 526 7.83e-159 463
MS.gene063696.t1 MTR_3g008720 46.498 514 245 6 36 541 36 527 8.93e-159 463
MS.gene063696.t1 MTR_3g008700 46.693 514 244 8 36 541 36 527 3.95e-158 461
MS.gene063696.t1 MTR_7g053360 46.109 514 247 7 36 541 36 527 6.73e-158 461
MS.gene063696.t1 MTR_4g025670 49.895 475 204 8 74 541 42 489 6.03e-157 457
MS.gene063696.t1 MTR_7g108905 45.753 518 253 7 35 537 48 552 7.88e-154 451
MS.gene063696.t1 MTR_7g050870 45.920 527 244 11 37 541 47 554 1.12e-152 448
MS.gene063696.t1 MTR_8g023310 44.381 525 268 6 35 541 71 589 2.12e-149 441
MS.gene063696.t1 MTR_1g008140 42.214 533 272 10 35 541 85 607 1.27e-141 422
MS.gene063696.t1 MTR_7g050950 43.762 521 253 10 42 541 52 553 1.71e-141 420
MS.gene063696.t1 MTR_8g042900 50.321 467 199 9 88 541 135 581 5.25e-141 419
MS.gene063696.t1 MTR_2g044880 46.849 476 219 7 79 541 71 525 6.13e-141 417
MS.gene063696.t1 MTR_3g091380 42.243 535 263 11 35 541 81 597 1.28e-140 419
MS.gene063696.t1 MTR_7g108900 45.047 535 251 11 33 537 35 556 5.05e-140 416
MS.gene063696.t1 MTR_7g050980 45.714 490 237 8 57 541 90 555 1.16e-139 415
MS.gene063696.t1 MTR_1g083520 45.698 523 243 14 35 541 31 528 2.62e-139 413
MS.gene063696.t1 MTR_4g087980 45.720 479 215 8 81 541 65 516 7.86e-137 407
MS.gene063696.t1 MTR_7g112580 44.848 495 235 10 63 541 70 542 2.08e-136 406
MS.gene063696.t1 MTR_7g108890 50.465 430 191 8 129 541 138 562 1.52e-133 400
MS.gene063696.t1 MTR_7g108875 42.750 531 273 8 35 541 41 564 1.81e-133 400
MS.gene063696.t1 MTR_3g010780 46.537 462 216 8 92 541 132 574 1.61e-132 397
MS.gene063696.t1 MTR_4g124550 41.777 529 271 10 33 541 82 593 1.67e-131 395
MS.gene063696.t1 MTR_1g088020 42.473 558 277 12 18 541 8 555 4.69e-131 393
MS.gene063696.t1 MTR_8g023150 43.238 525 256 13 36 541 81 582 1.22e-129 390
MS.gene063696.t1 MTR_8g042910 41.667 552 261 12 35 541 77 612 3.46e-129 390
MS.gene063696.t1 MTR_4g087970 43.552 473 236 6 81 541 64 517 5.87e-129 386
MS.gene063696.t1 MTR_1g054880 41.561 551 285 12 21 541 15 558 1.39e-128 387
MS.gene063696.t1 MTR_2g044810 40.625 512 276 10 36 541 45 534 9.04e-128 384
MS.gene063696.t1 MTR_1g088225 42.115 558 277 13 18 541 8 553 6.53e-126 380
MS.gene063696.t1 MTR_4g124520 41.954 522 243 14 36 541 44 521 2.06e-124 375
MS.gene063696.t1 MTR_1g086210 40.147 543 290 12 27 541 34 569 2.51e-123 374
MS.gene063696.t1 MTR_0458s0030 61.597 263 100 1 279 541 53 314 1.17e-118 353
MS.gene063696.t1 MTR_1g088040 61.597 263 100 1 279 541 53 314 1.17e-118 353
MS.gene063696.t1 MTR_1g086190 38.158 532 304 11 30 541 37 563 2.41e-118 361
MS.gene063696.t1 MTR_3g010770 43.764 441 220 7 124 541 140 575 9.86e-118 360
MS.gene063696.t1 MTR_2g044660 40.883 521 240 12 83 541 118 632 1.67e-117 361
MS.gene063696.t1 MTR_1g083470 38.921 519 286 14 36 537 62 566 1.80e-110 341
MS.gene063696.t1 MTR_1g083500 38.921 519 286 14 36 537 62 566 1.80e-110 341
MS.gene063696.t1 MTR_1g083510 37.569 543 281 14 36 540 60 582 2.12e-110 341
MS.gene063696.t1 MTR_7g112590 37.757 535 288 12 36 540 63 582 6.67e-110 340
MS.gene063696.t1 MTR_4g120290 38.964 521 290 12 36 540 63 571 4.65e-109 337
MS.gene063696.t1 MTR_1g083490 37.572 519 293 12 36 537 67 571 6.89e-109 337
MS.gene063696.t1 MTR_4g120280 38.593 526 291 12 33 540 60 571 9.13e-109 337
MS.gene063696.t1 MTR_4g005170 37.911 517 294 12 36 537 67 571 2.42e-108 337
MS.gene063696.t1 MTR_4g005180 36.802 519 297 12 36 537 49 553 3.27e-105 327
MS.gene063696.t1 MTR_7g110490 39.308 491 265 9 75 536 83 569 5.60e-104 324
MS.gene063696.t1 MTR_7g110510 39.308 491 265 9 75 536 83 569 5.60e-104 324
MS.gene063696.t1 MTR_2g436040 35.687 524 293 12 33 537 44 542 5.77e-102 320
MS.gene063696.t1 MTR_7g110440 36.059 538 309 10 33 541 43 574 1.34e-101 318
MS.gene063696.t1 MTR_7g110560 34.674 522 301 9 36 541 52 549 6.62e-99 310
MS.gene063696.t1 MTR_2g044810 53.360 253 117 1 289 541 214 465 8.70e-98 304
MS.gene063696.t1 MTR_2g436050 34.457 534 297 12 33 540 57 563 1.21e-92 294
MS.gene063696.t1 MTR_8g093460 34.535 527 298 13 31 533 40 543 8.61e-91 289
MS.gene063696.t1 MTR_3g095920 35.196 537 281 16 35 534 51 557 1.19e-89 286
MS.gene063696.t1 MTR_7g110420 37.500 480 268 9 75 524 83 560 2.58e-86 278
MS.gene063696.t1 MTR_5g083620 41.520 342 193 6 202 541 20 356 1.94e-82 261
MS.gene063696.t1 MTR_8g088610 39.466 337 194 4 203 537 44 372 2.73e-82 261
MS.gene063696.t1 MTR_7g110430 34.356 489 258 9 75 536 83 535 6.00e-78 255
MS.gene063696.t1 MTR_7g107180 39.936 313 175 5 230 541 7 307 1.48e-75 241
MS.gene063696.t1 MTR_2g105560 38.217 314 179 5 229 541 40 339 1.90e-74 239
MS.gene063696.t1 MTR_0458s0010 34.050 558 287 16 18 541 8 518 2.03e-71 237
MS.gene063696.t1 MTR_7g111560 31.530 536 294 17 36 541 64 556 1.66e-67 228
MS.gene063696.t1 MTR_2g039530 40.705 312 157 9 203 508 30 319 2.03e-57 195
MS.gene063696.t1 MTR_5g083590 34.286 315 155 5 228 541 5 268 7.12e-56 189
MS.gene063696.t1 MTR_6g024165 36.446 332 177 10 210 530 26 334 4.50e-53 183
MS.gene063696.t1 MTR_6g088810 39.085 284 147 7 258 530 109 377 5.10e-50 176
MS.gene063696.t1 MTR_8g102380 38.811 286 147 7 258 530 107 377 2.84e-49 174
MS.gene063696.t1 MTR_1g086540 32.733 333 193 8 208 530 33 344 4.07e-49 173
MS.gene063696.t1 MTR_6g012800 33.770 305 177 6 230 524 6 295 1.76e-48 171
MS.gene063696.t1 MTR_6g042660 34.722 288 164 6 221 503 28 296 8.63e-48 169
MS.gene063696.t1 MTR_7g092780 34.125 337 195 7 209 531 65 388 2.05e-47 170
MS.gene063696.t1 MTR_7g011530 32.099 324 189 7 232 539 8 316 1.35e-45 163
MS.gene063696.t1 MTR_1g033050 32.624 282 159 6 261 534 48 306 1.20e-42 155
MS.gene063696.t1 MTR_8g102380 40.000 230 113 5 258 474 107 324 1.15e-41 152
MS.gene063696.t1 MTR_6g042690 34.483 290 157 8 226 503 35 303 3.96e-41 151
MS.gene063696.t1 MTR_7g102600 30.719 306 192 5 232 530 101 393 1.39e-40 151
MS.gene063696.t1 MTR_5g095490 32.573 307 179 7 236 530 67 357 2.84e-37 141
MS.gene063696.t1 MTR_4g124740 31.833 311 177 12 230 530 54 339 3.74e-37 140
MS.gene063696.t1 MTR_5g088280 31.596 307 185 7 227 531 36 319 1.74e-35 135
MS.gene063696.t1 MTR_5g093970 30.363 303 195 6 230 531 43 330 2.71e-35 135
MS.gene063696.t1 MTR_5g088300 32.237 304 181 7 230 531 37 317 4.47e-35 134
MS.gene063696.t1 MTR_5g088290 30.693 303 186 6 230 530 48 328 6.53e-35 134
MS.gene063696.t1 MTR_5g012600 29.483 329 200 8 223 539 56 364 1.07e-33 131
MS.gene063696.t1 MTR_2g033970 29.231 325 204 7 212 530 42 346 1.86e-33 130
MS.gene063696.t1 MTR_8g036225 29.114 316 195 8 225 531 28 323 4.98e-31 123
MS.gene063696.t1 MTR_4g051810 30.033 303 190 7 230 531 37 318 9.33e-31 122
MS.gene063696.t1 MTR_2g073390 30.769 286 174 9 225 502 67 336 4.82e-30 121
MS.gene063696.t1 MTR_4g074290 29.603 277 176 6 230 500 71 334 2.11e-29 119
MS.gene063696.t1 MTR_0009s0350 29.474 285 170 8 230 502 71 336 4.91e-28 115
MS.gene063696.t1 MTR_0009s0340 29.474 285 170 8 230 502 71 336 4.91e-28 115
MS.gene063696.t1 MTR_2g436600 28.221 326 197 10 212 530 41 336 5.63e-28 115
MS.gene063696.t1 MTR_6g025590 34.921 189 109 7 210 390 34 216 1.36e-24 102
MS.gene063696.t1 MTR_2g436070 27.273 286 171 9 33 302 57 321 1.29e-20 94.4
MS.gene063696.t1 MTR_0408s0030 40.404 99 46 3 407 503 36 123 1.55e-15 74.3
MS.gene063696.t1 MTR_4g130790 28.313 166 109 3 261 419 55 217 2.58e-15 76.6
MS.gene063696.t1 MTR_7g102580 43.023 86 39 2 305 385 73 153 6.84e-13 67.0
MS.gene063696.t1 MTR_4g035880 28.313 166 103 5 27 181 35 195 7.93e-11 62.0
MS.gene063696.t1 MTR_4g035870 28.313 166 103 5 27 181 35 195 8.39e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063696.t1 AT1G23200 52.783 557 224 13 11 541 11 554 0.0 551
MS.gene063696.t1 AT2G45220 50.392 510 217 11 36 541 34 511 1.04e-173 501
MS.gene063696.t1 AT5G51490 43.570 521 257 9 36 541 38 536 1.76e-148 437
MS.gene063696.t1 AT3G49220 45.019 522 257 13 35 541 90 596 1.40e-147 437
MS.gene063696.t1 AT5G51500 43.846 520 257 10 36 541 42 540 5.02e-147 434
MS.gene063696.t1 AT3G49220 45.019 522 257 13 35 541 135 641 5.12e-147 437
MS.gene063696.t1 AT3G60730 45.253 495 239 7 70 541 34 519 8.22e-147 432
MS.gene063696.t1 AT5G53370 44.015 518 269 10 35 541 78 585 9.30e-145 429
MS.gene063696.t1 AT5G53370 44.015 518 269 10 35 541 114 621 2.13e-144 430
MS.gene063696.t1 AT3G47400 43.021 523 265 8 36 541 88 594 2.17e-144 429
MS.gene063696.t1 AT3G14310 43.623 541 261 9 35 541 62 592 5.11e-144 427
MS.gene063696.t1 AT1G11580 44.246 504 260 6 45 541 68 557 1.02e-141 421
MS.gene063696.t1 AT1G11580 44.246 504 260 6 45 541 89 578 1.66e-141 421
MS.gene063696.t1 AT1G53830 42.509 534 267 8 36 541 66 587 1.07e-138 414
MS.gene063696.t1 AT3G59010 46.170 483 227 11 69 541 69 528 1.24e-136 407
MS.gene063696.t1 AT3G43270 45.979 485 241 6 69 541 50 525 2.53e-135 403
MS.gene063696.t1 AT4G00190 42.967 519 246 11 28 541 1 474 1.31e-133 397
MS.gene063696.t1 AT2G26440 42.360 517 277 8 36 541 41 547 2.70e-133 399
MS.gene063696.t1 AT4G02330 42.004 569 279 12 18 541 9 571 1.95e-132 397
MS.gene063696.t1 AT5G27870 41.586 517 276 11 36 541 61 562 4.52e-131 399
MS.gene063696.t1 AT4G02320 45.395 456 229 5 96 541 73 518 5.83e-131 392
MS.gene063696.t1 AT3G05620 41.935 527 261 9 35 541 40 541 5.96e-131 392
MS.gene063696.t1 AT2G26450 41.586 517 273 9 36 541 113 611 7.38e-131 395
MS.gene063696.t1 AT4G02300 43.818 461 223 7 98 541 91 532 5.49e-130 390
MS.gene063696.t1 AT4G33220 42.585 472 237 6 94 541 62 523 4.71e-129 387
MS.gene063696.t1 AT3G05610 41.154 520 276 11 36 541 62 565 5.22e-129 392
MS.gene063696.t1 AT2G43050 45.393 445 209 7 103 541 100 516 6.64e-129 386
MS.gene063696.t1 AT5G04960 41.420 507 278 9 36 539 72 562 3.01e-128 386
MS.gene063696.t1 AT3G10710 41.748 515 264 9 36 541 74 561 2.95e-125 379
MS.gene063696.t1 AT5G04970 40.183 545 279 13 34 541 86 620 3.55e-123 375
MS.gene063696.t1 AT3G10720 38.961 539 292 9 35 541 82 615 4.07e-123 375
MS.gene063696.t1 AT1G02810 55.205 317 138 3 228 541 262 577 4.15e-123 374
MS.gene063696.t1 AT4G33220 50.442 339 162 2 203 541 70 402 1.65e-122 366
MS.gene063696.t1 AT2G47550 45.114 481 231 10 88 541 84 558 3.03e-122 370
MS.gene063696.t1 AT5G49180 40.344 523 282 13 33 537 56 566 9.43e-122 370
MS.gene063696.t1 AT4G33230 40.000 520 286 10 36 541 99 606 2.51e-121 370
MS.gene063696.t1 AT3G06830 39.771 523 285 14 36 539 55 566 1.00e-120 367
MS.gene063696.t1 AT1G53840 39.469 527 270 14 36 535 77 581 1.74e-120 367
MS.gene063696.t1 AT5G20860 39.279 527 272 12 36 541 39 538 2.11e-120 365
MS.gene063696.t1 AT3G14300 39.810 525 271 14 36 536 463 966 1.00e-112 357
MS.gene063696.t1 AT3G10720 56.538 260 112 1 282 541 1 259 3.98e-108 324
MS.gene063696.t1 AT4G03930 35.741 540 305 11 20 541 9 524 7.76e-105 325
MS.gene063696.t1 AT3G27980 47.181 337 170 3 205 541 169 497 1.97e-102 317
MS.gene063696.t1 AT1G11590 39.686 446 240 7 106 541 98 524 3.25e-101 315
MS.gene063696.t1 AT4G15980 46.746 338 176 4 203 539 366 700 1.60e-100 318
MS.gene063696.t1 AT4G15980 46.746 338 176 4 203 539 376 710 2.80e-100 318
MS.gene063696.t1 AT5G20860 36.047 516 252 12 28 520 29 489 2.68e-99 310
MS.gene063696.t1 AT5G09760 34.652 531 293 12 33 534 72 577 1.10e-91 292
MS.gene063696.t1 AT5G09760 34.652 531 293 12 33 534 46 551 1.15e-91 291
MS.gene063696.t1 AT1G11370 49.225 258 130 1 216 473 32 288 1.43e-86 269
MS.gene063696.t1 AT2G47030 39.563 412 218 8 154 541 184 588 5.23e-85 275
MS.gene063696.t1 AT2G47040 38.767 454 250 12 103 541 155 595 7.35e-85 275
MS.gene063696.t1 AT3G62170 36.709 474 259 11 100 541 124 588 9.36e-85 274
MS.gene063696.t1 AT5G64640 36.971 449 249 11 104 531 164 599 2.78e-84 273
MS.gene063696.t1 AT5G19730 37.676 284 151 6 258 530 113 381 3.01e-50 177
MS.gene063696.t1 AT3G29090 35.439 285 160 6 230 503 7 278 5.36e-48 169
MS.gene063696.t1 AT1G05310 34.713 314 176 7 230 530 91 388 2.00e-46 167
MS.gene063696.t1 AT2G36700 31.579 304 189 5 232 530 44 333 1.43e-43 158
MS.gene063696.t1 AT2G36710 33.117 308 186 5 230 530 91 385 1.92e-43 159
MS.gene063696.t1 AT1G44980 42.029 207 118 2 188 394 37 241 2.38e-41 149
MS.gene063696.t1 AT2G21610 31.126 302 187 6 230 530 51 332 5.47e-40 149
MS.gene063696.t1 AT2G21610 31.126 302 187 6 230 530 56 337 8.52e-40 148
MS.gene063696.t1 AT3G17060 29.167 336 204 8 210 530 20 336 9.02e-40 148
MS.gene063696.t1 AT3G24130 31.333 300 179 7 215 503 21 304 2.40e-38 144
MS.gene063696.t1 AT5G18990 32.667 300 170 7 215 503 21 299 1.20e-37 142
MS.gene063696.t1 AT5G47500 30.699 329 196 8 223 539 54 362 3.82e-37 141
MS.gene063696.t1 AT5G07430 30.323 310 196 6 225 530 62 355 1.46e-36 139
MS.gene063696.t1 AT5G55590 34.069 317 179 9 227 530 79 378 1.89e-35 137
MS.gene063696.t1 AT5G26810 30.794 315 191 9 230 531 34 334 8.87e-34 131
MS.gene063696.t1 AT5G55590 34.231 260 146 7 227 474 79 325 1.31e-33 130
MS.gene063696.t1 AT3G49220 33.219 292 165 13 35 311 135 411 2.25e-33 132
MS.gene063696.t1 AT2G19150 28.395 324 209 8 221 537 32 339 1.20e-32 128
MS.gene063696.t1 AT5G61680 29.052 327 206 9 209 530 53 358 1.33e-32 129
MS.gene063696.t1 AT5G07420 28.308 325 207 9 213 530 50 355 5.49e-31 124
MS.gene063696.t1 AT2G47280 29.487 312 192 8 230 531 35 328 6.14e-30 120
MS.gene063696.t1 AT2G19150 29.344 259 167 6 221 474 32 279 2.08e-28 115
MS.gene063696.t1 AT1G69940 26.045 311 207 8 225 530 63 355 8.43e-27 112
MS.gene063696.t1 AT5G07410 26.367 311 206 8 225 530 63 355 1.30e-26 111

Find 133 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGGTGATTCTTGGATTTC+AGG 0.138272 4.3:-1679237 MS.gene063696:CDS
AAATTTCCATACTGGCTTTC+AGG 0.138802 4.3:-1681204 MS.gene063696:CDS
GTGAAGAATTTAATGATATT+TGG 0.168927 4.3:-1680979 MS.gene063696:CDS
TTCTTCTTCTTTCCATGATA+TGG 0.204942 4.3:-1681628 MS.gene063696:CDS
AATAATTATTGCAGCTGTTA+TGG 0.230375 4.3:-1679889 MS.gene063696:intron
GCCAAGTTAATTTATCATTT+AGG 0.239565 4.3:+1681442 None:intergenic
GCATTGAGTACACTTTATTA+TGG 0.267204 4.3:-1679375 MS.gene063696:CDS
AATCATCAAACTTGTCAAAA+TGG 0.271720 4.3:-1681402 MS.gene063696:CDS
ACAATCTTCCCATGCTGATT+TGG 0.282004 4.3:+1681521 None:intergenic
TTTCCATGATATGGCTCTTA+AGG 0.287787 4.3:-1681619 MS.gene063696:CDS
AATCATTTGCTGCGGTTATA+CGG 0.315468 4.3:+1679532 None:intergenic
AATATGAGTTTATTCATATT+AGG 0.319561 4.3:+1681792 None:intergenic
CTGGTGATTCTTGGATTTCA+GGG 0.333928 4.3:-1679236 MS.gene063696:CDS
ATAATTATTGCAGCTGTTAT+GGG 0.345443 4.3:-1679888 MS.gene063696:intron
TCATCAAGTACTAAACAAAA+TGG 0.356786 4.3:-1681249 MS.gene063696:CDS
ATGGACTCCACTATTGTTAC+TGG 0.363587 4.3:-1680949 MS.gene063696:CDS
GGAACTATTGATTTCATCTT+CGG 0.369134 4.3:-1679684 MS.gene063696:CDS
CATGGAAAGAAGAAGAATCT+AGG 0.371037 4.3:+1681634 None:intergenic
TATCAGATAAATTTCCATAC+TGG 0.372948 4.3:-1681212 MS.gene063696:CDS
TTTGATAAATTCACAAGGTT+GGG 0.373566 4.3:-1679418 MS.gene063696:CDS
GTCAGATAGAAAGCTGCTTC+AGG 0.393140 4.3:-1681181 MS.gene063696:CDS
TCTGACCCTGAAAGCCAGTA+TGG 0.394838 4.3:+1681198 None:intergenic
AATTCAGTTAAAACATATCT+TGG 0.398697 4.3:-1679495 MS.gene063696:CDS
CTTCAGGCAACACCAGGGTC+AGG 0.401448 4.3:-1681165 MS.gene063696:CDS
AATTTCCATACTGGCTTTCA+GGG 0.402050 4.3:-1681203 MS.gene063696:CDS
GGTAATCATAATGCTATAGA+TGG 0.406520 4.3:-1680928 MS.gene063696:CDS
TGTTGCAGTCGCGGTAGAAT+TGG 0.411073 4.3:+1679712 None:intergenic
GTAATGAAAAGTAATCTTGA+TGG 0.411444 4.3:-1679441 MS.gene063696:CDS
GTTGTCATTCATGTGAAATC+AGG 0.412213 4.3:-1681036 MS.gene063696:CDS
TGTTCTTCCTTGTGCTGTTA+CGG 0.414972 4.3:+1679593 None:intergenic
ATACTGGTGGAAGAGTTAAT+TGG 0.417090 4.3:-1679323 MS.gene063696:CDS
GTTTGATAAATTCACAAGGT+TGG 0.422089 4.3:-1679419 MS.gene063696:CDS
AGACAGTGTGTTGGTGGTGG+TGG 0.423692 4.3:+1681659 None:intergenic
GCGGTTGGCGTAAGCATATA+AGG 0.426322 4.3:+1679734 None:intergenic
CGCAGCAAATGATTTGAAAC+CGG 0.431620 4.3:-1679523 MS.gene063696:CDS
TACAAGTTTGTGTGCTTCAA+TGG 0.434838 4.3:+1681581 None:intergenic
GTTGGTGGTGGTGCTAATAC+TGG 0.437055 4.3:-1679339 MS.gene063696:CDS
CTTGGTGCTGGCCCTGACCC+TGG 0.440453 4.3:+1681153 None:intergenic
CAAGCAGTAGCTCTCCGTTC+CGG 0.442034 4.3:-1679807 MS.gene063696:CDS
GGGAAGTAGTTTAAGTGAGA+TGG 0.442427 4.3:+1681360 None:intergenic
GGTGTCTTGGTAGCCTTTGA+AGG 0.444283 4.3:+1679755 None:intergenic
TTTGAAATTATTTAATTCCA+TGG 0.450557 4.3:+1681554 None:intergenic
GTGTTCTGTTGGTTGCTCAT+TGG 0.453378 4.3:+1679615 None:intergenic
ACAAGGTTGGGCTCCATGGA+TGG 0.456078 4.3:-1679406 MS.gene063696:CDS
TGGTTACACACATGAGGGTA+TGG 0.456641 4.3:+1681693 None:intergenic
GTGAAGTTGCTTAACATGGA+TGG 0.463236 4.3:+1681339 None:intergenic
CAAGGTTGGGCTCCATGGAT+GGG 0.470284 4.3:-1679405 MS.gene063696:CDS
ATTCACAAGGTTGGGCTCCA+TGG 0.471108 4.3:-1679410 MS.gene063696:CDS
ATTGCAGCTGTTATGGGAGA+TGG 0.472604 4.3:-1679882 MS.gene063696:intron
AATGATATTTGGAGATGGAA+TGG 0.477734 4.3:-1680968 MS.gene063696:CDS
GTTGCAGCTGCTAAGCTTAG+TGG 0.477922 4.3:-1681069 MS.gene063696:CDS
GTTTGTGTGCTTCAATGGCT+TGG 0.481327 4.3:+1681586 None:intergenic
AAGCTGCTTCAGGCAACACC+AGG 0.481870 4.3:-1681171 MS.gene063696:CDS
GGACACGACCTTTGAGAATA+CGG 0.482090 4.3:-1679847 MS.gene063696:CDS
ACAAGTTTGATGATTGGTAA+TGG 0.484728 4.3:+1681410 None:intergenic
TGCTTCAATGGCTTGGTCCA+TGG 0.489763 4.3:+1681593 None:intergenic
TTCTACCGTTGTGCCTTCAA+AGG 0.490213 4.3:-1679768 MS.gene063696:CDS
AGATTGTTTAGAGCTTTATG+AGG 0.504717 4.3:-1681505 MS.gene063696:CDS
AGTGGTGTGCCGTTTGATGC+AGG 0.508356 4.3:-1679213 MS.gene063696:CDS
ACCTAAATGATAAATTAACT+TGG 0.508748 4.3:-1681443 MS.gene063696:CDS
ACAATATCAGCTCTTGGTGC+TGG 0.510769 4.3:+1681141 None:intergenic
TGCAGTCGCGGTAGAATTGG+CGG 0.511251 4.3:+1679715 None:intergenic
TGAAGTTGCTTAACATGGAT+GGG 0.511299 4.3:+1681340 None:intergenic
TTGCAGCTGCTAAGCTTAGT+GGG 0.511688 4.3:-1681068 MS.gene063696:CDS
GGTAGACAGTGTGTTGGTGG+TGG 0.512332 4.3:+1681656 None:intergenic
GATATTGTTGTTGCACAAGA+TGG 0.515434 4.3:-1681126 MS.gene063696:CDS
TGGTGGTGGTTCCAATGTAA+TGG 0.517543 4.3:+1681673 None:intergenic
GTTCTTGAATATTTCTGCCA+TGG 0.518439 4.3:+1679468 None:intergenic
GGTGGAAGAGTTAATTGGCC+TGG 0.520232 4.3:-1679318 MS.gene063696:CDS
ATGATTACCAGTAACAATAG+TGG 0.522826 4.3:+1680942 None:intergenic
ATGGAAAGAAGAAGAATCTA+GGG 0.522868 4.3:+1681635 None:intergenic
GGGAGATGGTTTCATAGCTA+AGG 0.523196 4.3:-1679868 MS.gene063696:CDS
GAAGGCACAACGGTAGAAAA+CGG 0.523739 4.3:+1679773 None:intergenic
ATTCACAAACCTGCATCAAA+CGG 0.526344 4.3:+1679204 None:intergenic
TTTAGTGAAGTTGCTTAACA+TGG 0.527598 4.3:+1681335 None:intergenic
TACCGCGACTGCAACATCTA+CGG 0.528232 4.3:-1679705 MS.gene063696:CDS
TGACCTTAAGAGCCATATCA+TGG 0.529885 4.3:+1681616 None:intergenic
CGTAAGCATATAAGGTGTCT+TGG 0.533953 4.3:+1679742 None:intergenic
GGTTGGGCTCCATGGATGGG+TGG 0.536463 4.3:-1679402 MS.gene063696:CDS
TGTTATTACTAATCCTTCTG+AGG 0.537907 4.3:-1679289 MS.gene063696:CDS
GTGCTGTTACGGTGTTCTGT+TGG 0.539536 4.3:+1679604 None:intergenic
TGAAGTTAGAGTGTTGCTAA+TGG 0.541067 4.3:+1681293 None:intergenic
TATGGTGAATATATGAATGT+TGG 0.547151 4.3:-1679357 MS.gene063696:CDS
GGTTACACACATGAGGGTAT+GGG 0.553090 4.3:+1681694 None:intergenic
GAAAATTTAACAGCCTCAGA+AGG 0.553132 4.3:+1679276 None:intergenic
AATTTAATGATATTTGGAGA+TGG 0.553852 4.3:-1680973 MS.gene063696:CDS
CAGAACACCGTAACAGCACA+AGG 0.556039 4.3:-1679600 MS.gene063696:CDS
GGGTCCTGCCGTATTCTCAA+AGG 0.556845 4.3:+1679839 None:intergenic
GCTGCTAAGCTTAGTGGGAA+AGG 0.557812 4.3:-1681063 MS.gene063696:CDS
GATTCTTGGATTTCAGGGAG+TGG 0.559486 4.3:-1679231 MS.gene063696:CDS
CGGAGTGATCGGCGCCGGAA+CGG 0.559791 4.3:+1679793 None:intergenic
GCAACAACAATATCAGCTCT+TGG 0.560130 4.3:+1681135 None:intergenic
AGTACTTGATGATGTGGATG+AGG 0.565120 4.3:+1681260 None:intergenic
TTGTTTAGTACTTGATGATG+TGG 0.565122 4.3:+1681254 None:intergenic
GTTGTTGCACAAGATGGAAG+TGG 0.568022 4.3:-1681120 MS.gene063696:CDS
ACGGTAGAAAACGGAGTGAT+CGG 0.568769 4.3:+1679782 None:intergenic
CATGTGTGTAACCATTACAT+TGG 0.569476 4.3:-1681684 MS.gene063696:CDS
GTCGCGGTAGAATTGGCGGT+TGG 0.570719 4.3:+1679719 None:intergenic
AGTAGTTTAAGTGAGATGGA+AGG 0.574197 4.3:+1681364 None:intergenic
GTTACACACATGAGGGTATG+GGG 0.574452 4.3:+1681695 None:intergenic
TAGGGTAGACAGTGTGTTGG+TGG 0.577198 4.3:+1681653 None:intergenic
CGGAACGGAGAGCTACTGCT+TGG 0.579824 4.3:+1679808 None:intergenic
AATGCAAAACCACCCATCCA+TGG 0.580980 4.3:+1679393 None:intergenic
TGCAACAACACCCTCTGAGA+TGG 0.581283 4.3:+1681086 None:intergenic
GAAAACGGAGTGATCGGCGC+CGG 0.581382 4.3:+1679788 None:intergenic
TTCCGTAGATGTTGCAGTCG+CGG 0.586032 4.3:+1679703 None:intergenic
TCAAGTACTAAACAAAATGG+AGG 0.588650 4.3:-1681246 MS.gene063696:CDS
GGTGAATATATGAATGTTGG+TGG 0.591996 4.3:-1679354 MS.gene063696:CDS
ACAAGCGTGCCAAATCAGCA+TGG 0.597886 4.3:-1681530 MS.gene063696:CDS
AGGATATGCAATATTGTGCA+TGG 0.599200 4.3:+1681812 None:intergenic
AACTATAAGACCATCTCAGA+GGG 0.599247 4.3:-1681096 MS.gene063696:CDS
ACGACCTTTGAGAATACGGC+AGG 0.599978 4.3:-1679843 MS.gene063696:CDS
TTCAGGCAACACCAGGGTCA+GGG 0.603563 4.3:-1681164 MS.gene063696:CDS
CAAGCGTGCCAAATCAGCAT+GGG 0.604901 4.3:-1681529 MS.gene063696:CDS
ATGTAATGGTTACACACATG+AGG 0.605826 4.3:+1681687 None:intergenic
GATGATGTGGATGAGGATGA+TGG 0.609727 4.3:+1681267 None:intergenic
ATCTAGGGTAGACAGTGTGT+TGG 0.618112 4.3:+1681650 None:intergenic
CTAAACAAAATGGAGGGAGA+AGG 0.620123 4.3:-1681239 MS.gene063696:CDS
GAATATATGAATGTTGGTGG+TGG 0.621736 4.3:-1679351 MS.gene063696:CDS
CGGTTTCAAATCATTTGCTG+CGG 0.623680 4.3:+1679524 None:intergenic
CAAGTACTAAACAAAATGGA+GGG 0.628274 4.3:-1681245 MS.gene063696:CDS
TGTAATGGTTACACACATGA+GGG 0.637885 4.3:+1681688 None:intergenic
ACAGATCCAAATGAAAACAC+AGG 0.641536 4.3:-1679573 MS.gene063696:CDS
AAACATATCTTGGTAGACCA+TGG 0.646826 4.3:-1679485 MS.gene063696:CDS
GGTGGTGGTGCTAATACTGG+TGG 0.661832 4.3:-1679336 MS.gene063696:CDS
ATGGCAGAAATATTCAAGAA+CGG 0.671890 4.3:-1679466 MS.gene063696:CDS
TTAGTAATAACATGAAAACC+AGG 0.674041 4.3:+1679300 None:intergenic
ACACAAACTTGTATCAACCA+TGG 0.676347 4.3:-1681571 MS.gene063696:CDS
TATGGCTCTTAAGGTCACCA+TGG 0.681394 4.3:-1681610 MS.gene063696:CDS
GGTAGCCTTTGAAGGCACAA+CGG 0.688392 4.3:+1679763 None:intergenic
AGCTGCTTCAGGCAACACCA+GGG 0.695457 4.3:-1681170 MS.gene063696:CDS
GATGGTTTGATAAATTCACA+AGG 0.696704 4.3:-1679423 MS.gene063696:CDS
AAACTATAAGACCATCTCAG+AGG 0.701800 4.3:-1681097 MS.gene063696:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACAAATGTTAAATAAAGT+AGG + chr4.3:1680539-1680558 None:intergenic 15.0%
!! AACAAATGTTAAATAAAGTA+GGG + chr4.3:1680538-1680557 None:intergenic 15.0%
!! AATATGAGTTTATTCATATT+AGG + chr4.3:1679227-1679246 None:intergenic 15.0%
!! TTTAGTGAATATTGTTAAAA+AGG + chr4.3:1680869-1680888 None:intergenic 15.0%
!!! TTTGAAATTATTTAATTCCA+TGG + chr4.3:1679465-1679484 None:intergenic 15.0%
!! AATTCAGTTAAAACATATCT+TGG - chr4.3:1681521-1681540 MS.gene063696:CDS 20.0%
!! AATTTAATGATATTTGGAGA+TGG - chr4.3:1680043-1680062 MS.gene063696:intron 20.0%
!! ACCTAAATGATAAATTAACT+TGG - chr4.3:1679573-1679592 MS.gene063696:CDS 20.0%
!! GGTCATAATTACTTTATTAA+AGG + chr4.3:1681002-1681021 None:intergenic 20.0%
!! GTGAAGAATTTAATGATATT+TGG - chr4.3:1680037-1680056 MS.gene063696:intron 20.0%
!! TATGAATAGATTAGTGATTA+AGG + chr4.3:1680157-1680176 None:intergenic 20.0%
!!! AACTTTTTACATTAATTGAC+CGG + chr4.3:1680916-1680935 None:intergenic 20.0%
!!! ATTTTCTGTTGGTAATTTTT+TGG - chr4.3:1681757-1681776 MS.gene063696:CDS 20.0%
! AACAATATTCACTAAAGTTC+TGG - chr4.3:1680873-1680892 MS.gene063696:intron 25.0%
! AATAATTATTGCAGCTGTTA+TGG - chr4.3:1681127-1681146 MS.gene063696:CDS 25.0%
! AATCATCAAACTTGTCAAAA+TGG - chr4.3:1679614-1679633 MS.gene063696:CDS 25.0%
! ATAATTATTGCAGCTGTTAT+GGG - chr4.3:1681128-1681147 MS.gene063696:CDS 25.0%
! CACTAGAAAATATATCACTT+TGG - chr4.3:1680318-1680337 MS.gene063696:intron 25.0%
! GCCAAGTTAATTTATCATTT+AGG + chr4.3:1679577-1679596 None:intergenic 25.0%
! GTAATGAAAAGTAATCTTGA+TGG - chr4.3:1681575-1681594 MS.gene063696:CDS 25.0%
! TATCAGATAAATTTCCATAC+TGG - chr4.3:1679804-1679823 MS.gene063696:CDS 25.0%
! TATGGTGAATATATGAATGT+TGG - chr4.3:1681659-1681678 MS.gene063696:CDS 25.0%
! TCATCAAGTACTAAACAAAA+TGG - chr4.3:1679767-1679786 MS.gene063696:CDS 25.0%
! TTAGTAATAACATGAAAACC+AGG + chr4.3:1681719-1681738 None:intergenic 25.0%
! TTTATGTACAACTTGTGTTA+AGG - chr4.3:1680371-1680390 MS.gene063696:intron 25.0%
! TTTGATAAATTCACAAGGTT+GGG - chr4.3:1681598-1681617 MS.gene063696:CDS 25.0%
!! GATGTAAAAGGTATAATTGT+TGG + chr4.3:1680726-1680745 None:intergenic 25.0%
!!! ATTTTGACAAGTTTGATGAT+TGG + chr4.3:1679615-1679634 None:intergenic 25.0%
!!! GTAAAGAATTTTTTGCACTA+TGG + chr4.3:1681060-1681079 None:intergenic 25.0%
AATGATATTTGGAGATGGAA+TGG - chr4.3:1680048-1680067 MS.gene063696:intron 30.0%
ATGATTACCAGTAACAATAG+TGG + chr4.3:1680077-1680096 None:intergenic 30.0%
ATGGAAAGAAGAAGAATCTA+GGG + chr4.3:1679384-1679403 None:intergenic 30.0%
ATGGCAGAAATATTCAAGAA+CGG - chr4.3:1681550-1681569 MS.gene063696:CDS 30.0%
CAAGTACTAAACAAAATGGA+GGG - chr4.3:1679771-1679790 MS.gene063696:CDS 30.0%
CCACATACTATTAAATGTTC+AGG - chr4.3:1681020-1681039 MS.gene063696:CDS 30.0%
CCTGAACATTTAATAGTATG+TGG + chr4.3:1681023-1681042 None:intergenic 30.0%
GATGGTTTGATAAATTCACA+AGG - chr4.3:1681593-1681612 MS.gene063696:CDS 30.0%
GCATTGAGTACACTTTATTA+TGG - chr4.3:1681641-1681660 MS.gene063696:CDS 30.0%
GGTAATCATAATGCTATAGA+TGG - chr4.3:1680088-1680107 MS.gene063696:intron 30.0%
GTTTGATAAATTCACAAGGT+TGG - chr4.3:1681597-1681616 MS.gene063696:CDS 30.0%
TCAAGTACTAAACAAAATGG+AGG - chr4.3:1679770-1679789 MS.gene063696:CDS 30.0%
TGTTATTACTAATCCTTCTG+AGG - chr4.3:1681727-1681746 MS.gene063696:CDS 30.0%
TTCTTCTTCTTTCCATGATA+TGG - chr4.3:1679388-1679407 MS.gene063696:CDS 30.0%
TTGAGTAACTACTTGTCATA+TGG - chr4.3:1680414-1680433 MS.gene063696:intron 30.0%
TTTAGTGAAGTTGCTTAACA+TGG + chr4.3:1679684-1679703 None:intergenic 30.0%
! ACAAGTTTGATGATTGGTAA+TGG + chr4.3:1679609-1679628 None:intergenic 30.0%
! ACAATTCCTGTGTTTTCATT+TGG + chr4.3:1681452-1681471 None:intergenic 30.0%
! AGATTGTTTAGAGCTTTATG+AGG - chr4.3:1679511-1679530 MS.gene063696:CDS 30.0%
! CTTTTCTACTTTCTCTCAAA+AGG - chr4.3:1680566-1680585 MS.gene063696:intron 30.0%
!! ACAGTTGTTTTCTATATGCA+TGG - chr4.3:1680224-1680243 MS.gene063696:intron 30.0%
!! CAGTTGTTTTCTATATGCAT+GGG - chr4.3:1680225-1680244 MS.gene063696:intron 30.0%
!! GGAACTATTGATTTCATCTT+CGG - chr4.3:1681332-1681351 MS.gene063696:CDS 30.0%
!! TTGTTTAGTACTTGATGATG+TGG + chr4.3:1679765-1679784 None:intergenic 30.0%
!!! GTTTTAACTGAATTCTGAAC+CGG + chr4.3:1681515-1681534 None:intergenic 30.0%
!!! TTTTCTGAGACTGATGTAAA+AGG + chr4.3:1680738-1680757 None:intergenic 30.0%
AAACATATCTTGGTAGACCA+TGG - chr4.3:1681531-1681550 MS.gene063696:CDS 35.0%
AAACTATAAGACCATCTCAG+AGG - chr4.3:1679919-1679938 MS.gene063696:intron 35.0%
AACTATAAGACCATCTCAGA+GGG - chr4.3:1679920-1679939 MS.gene063696:intron 35.0%
ACACAAACTTGTATCAACCA+TGG - chr4.3:1679445-1679464 MS.gene063696:CDS 35.0%
ACAGATCCAAATGAAAACAC+AGG - chr4.3:1681443-1681462 MS.gene063696:CDS 35.0%
ACTGCATGTCTCTATCTTAA+TGG + chr4.3:1680346-1680365 None:intergenic 35.0%
AGGTATAATTGTTGGTCGTA+TGG + chr4.3:1680718-1680737 None:intergenic 35.0%
AGTAGTTTAAGTGAGATGGA+AGG + chr4.3:1679655-1679674 None:intergenic 35.0%
ATACTGGTGGAAGAGTTAAT+TGG - chr4.3:1681693-1681712 MS.gene063696:CDS 35.0%
ATGTAATGGTTACACACATG+AGG + chr4.3:1679332-1679351 None:intergenic 35.0%
CATGGAAAGAAGAAGAATCT+AGG + chr4.3:1679385-1679404 None:intergenic 35.0%
CATGTGTGTAACCATTACAT+TGG - chr4.3:1679332-1679351 MS.gene063696:CDS 35.0%
CTGCATGTCTCTATCTTAAT+GGG + chr4.3:1680345-1680364 None:intergenic 35.0%
GAAAATTTAACAGCCTCAGA+AGG + chr4.3:1681743-1681762 None:intergenic 35.0%
GCACAAACAAGAGAGAAAAA+AGG + chr4.3:1679274-1679293 None:intergenic 35.0%
GGGAGAAATCATATGATCAA+TGG - chr4.3:1680245-1680264 MS.gene063696:intron 35.0%
GGTGAATATATGAATGTTGG+TGG - chr4.3:1681662-1681681 MS.gene063696:CDS 35.0%
GTTGTCATTCATGTGAAATC+AGG - chr4.3:1679980-1679999 MS.gene063696:intron 35.0%
TACAAGTTTGTGTGCTTCAA+TGG + chr4.3:1679438-1679457 None:intergenic 35.0%
TGAAGTTGCTTAACATGGAT+GGG + chr4.3:1679679-1679698 None:intergenic 35.0%
TGCATGTCTCTATCTTAATG+GGG + chr4.3:1680344-1680363 None:intergenic 35.0%
TGTAATGGTTACACACATGA+GGG + chr4.3:1679331-1679350 None:intergenic 35.0%
TTTCCATGATATGGCTCTTA+AGG - chr4.3:1679397-1679416 MS.gene063696:CDS 35.0%
! AATCATTTGCTGCGGTTATA+CGG + chr4.3:1681487-1681506 None:intergenic 35.0%
! GAATATATGAATGTTGGTGG+TGG - chr4.3:1681665-1681684 MS.gene063696:CDS 35.0%
! GAGGCTGTTAAATTTTCTGT+TGG - chr4.3:1681746-1681765 MS.gene063696:CDS 35.0%
! GATATTGTTGTTGCACAAGA+TGG - chr4.3:1679890-1679909 MS.gene063696:intron 35.0%
! GTTCTTGAATATTTCTGCCA+TGG + chr4.3:1681551-1681570 None:intergenic 35.0%
! TACATTAATTGACCGGTTCA+TGG + chr4.3:1680909-1680928 None:intergenic 35.0%
!! AAATTTCCATACTGGCTTTC+AGG - chr4.3:1679812-1679831 MS.gene063696:CDS 35.0%
!! AATTTCCATACTGGCTTTCA+GGG - chr4.3:1679813-1679832 MS.gene063696:CDS 35.0%
!! ACTTTTCGTTCAGCTACTTT+TGG - chr4.3:1680118-1680137 MS.gene063696:intron 35.0%
!! TGAAGTTAGAGTGTTGCTAA+TGG + chr4.3:1679726-1679745 None:intergenic 35.0%
!! TTTTCTCTCTTGTTTGTGCA+TGG - chr4.3:1679275-1679294 MS.gene063696:CDS 35.0%
!!! ATTTTTTGGCTGGTGATTCT+TGG - chr4.3:1681771-1681790 MS.gene063696:CDS 35.0%
!!! TCTGTTGGTAATTTTTTGGC+TGG - chr4.3:1681761-1681780 MS.gene063696:CDS 35.0%
ATGGACTCCACTATTGTTAC+TGG - chr4.3:1680067-1680086 MS.gene063696:intron 40.0%
CTAAACAAAATGGAGGGAGA+AGG - chr4.3:1679777-1679796 MS.gene063696:CDS 40.0%
CTGGTGATTCTTGGATTTCA+GGG - chr4.3:1681780-1681799 MS.gene063696:CDS 40.0%
GCAACAACAATATCAGCTCT+TGG + chr4.3:1679884-1679903 None:intergenic 40.0%
GGAGTTTAATCTCCATGAAC+CGG - chr4.3:1680894-1680913 MS.gene063696:intron 40.0%
GGGAAGTAGTTTAAGTGAGA+TGG + chr4.3:1679659-1679678 None:intergenic 40.0%
GTGAAGTTGCTTAACATGGA+TGG + chr4.3:1679680-1679699 None:intergenic 40.0%
TGACCTTAAGAGCCATATCA+TGG + chr4.3:1679403-1679422 None:intergenic 40.0%
! ACAATCTTCCCATGCTGATT+TGG + chr4.3:1679498-1679517 None:intergenic 40.0%
! CGCAGCAAATGATTTGAAAC+CGG - chr4.3:1681493-1681512 MS.gene063696:CDS 40.0%
! CGGTTTCAAATCATTTGCTG+CGG + chr4.3:1681495-1681514 None:intergenic 40.0%
! CGTAAGCATATAAGGTGTCT+TGG + chr4.3:1681277-1681296 None:intergenic 40.0%
! TGTTCTTCCTTGTGCTGTTA+CGG + chr4.3:1681426-1681445 None:intergenic 40.0%
!! AGTACTTGATGATGTGGATG+AGG + chr4.3:1679759-1679778 None:intergenic 40.0%
!! CGAAGATGTTGCAGTTTTGA+AGG + chr4.3:1681373-1681392 None:intergenic 40.0%
!!! AGCTACTGCTTGGTGTTTTT+GGG + chr4.3:1681201-1681220 None:intergenic 40.0%
AATGCAAAACCACCCATCCA+TGG + chr4.3:1681626-1681645 None:intergenic 45.0%
ACGGTAGAAAACGGAGTGAT+CGG + chr4.3:1681237-1681256 None:intergenic 45.0%
ATTGCAGCTGTTATGGGAGA+TGG - chr4.3:1681134-1681153 MS.gene063696:CDS 45.0%
GATGATGTGGATGAGGATGA+TGG + chr4.3:1679752-1679771 None:intergenic 45.0%
GATTCTTGGATTTCAGGGAG+TGG - chr4.3:1681785-1681804 MS.gene063696:CDS 45.0%
GCTGGTGATTCTTGGATTTC+AGG - chr4.3:1681779-1681798 MS.gene063696:CDS 45.0%
GGGAGATGGTTTCATAGCTA+AGG - chr4.3:1681148-1681167 MS.gene063696:CDS 45.0%
GTCAGATAGAAAGCTGCTTC+AGG - chr4.3:1679835-1679854 MS.gene063696:CDS 45.0%
GTTGTTGCACAAGATGGAAG+TGG - chr4.3:1679896-1679915 MS.gene063696:intron 45.0%
GTTTGTGTGCTTCAATGGCT+TGG + chr4.3:1679433-1679452 None:intergenic 45.0%
TATGGCTCTTAAGGTCACCA+TGG - chr4.3:1679406-1679425 MS.gene063696:CDS 45.0%
TGGTTACACACATGAGGGTA+TGG + chr4.3:1679326-1679345 None:intergenic 45.0%
TTCTACCGTTGTGCCTTCAA+AGG - chr4.3:1681248-1681267 MS.gene063696:CDS 45.0%
TTGCAGCTGCTAAGCTTAGT+GGG - chr4.3:1679948-1679967 MS.gene063696:intron 45.0%
! ACAATATCAGCTCTTGGTGC+TGG + chr4.3:1679878-1679897 None:intergenic 45.0%
! ATCTAGGGTAGACAGTGTGT+TGG + chr4.3:1679369-1679388 None:intergenic 45.0%
! GAAGGCACAACGGTAGAAAA+CGG + chr4.3:1681246-1681265 None:intergenic 45.0%
! GGACACGACCTTTGAGAATA+CGG - chr4.3:1681169-1681188 MS.gene063696:CDS 45.0%
! GGTTACACACATGAGGGTAT+GGG + chr4.3:1679325-1679344 None:intergenic 45.0%
! GTGTTCTGTTGGTTGCTCAT+TGG + chr4.3:1681404-1681423 None:intergenic 45.0%
! GTTACACACATGAGGGTATG+GGG + chr4.3:1679324-1679343 None:intergenic 45.0%
!! TGGTGGTGGTTCCAATGTAA+TGG + chr4.3:1679346-1679365 None:intergenic 45.0%
!!! GAGCTACTGCTTGGTGTTTT+TGG + chr4.3:1681202-1681221 None:intergenic 45.0%
!!! GCTACTGCTTGGTGTTTTTG+GGG + chr4.3:1681200-1681219 None:intergenic 45.0%
ACAAGCGTGCCAAATCAGCA+TGG - chr4.3:1679486-1679505 MS.gene063696:CDS 50.0%
ATTCACAAGGTTGGGCTCCA+TGG - chr4.3:1681606-1681625 MS.gene063696:CDS 50.0%
CAAGCGTGCCAAATCAGCAT+GGG - chr4.3:1679487-1679506 MS.gene063696:CDS 50.0%
CAGAACACCGTAACAGCACA+AGG - chr4.3:1681416-1681435 MS.gene063696:CDS 50.0%
GCGGTTGGCGTAAGCATATA+AGG + chr4.3:1681285-1681304 None:intergenic 50.0%
GCTGCTAAGCTTAGTGGGAA+AGG - chr4.3:1679953-1679972 MS.gene063696:intron 50.0%
GGTGGAAGAGTTAATTGGCC+TGG - chr4.3:1681698-1681717 MS.gene063696:CDS 50.0%
GTTGCAGCTGCTAAGCTTAG+TGG - chr4.3:1679947-1679966 MS.gene063696:intron 50.0%
TACCGCGACTGCAACATCTA+CGG - chr4.3:1681311-1681330 MS.gene063696:CDS 50.0%
TAGGGTAGACAGTGTGTTGG+TGG + chr4.3:1679366-1679385 None:intergenic 50.0%
TCTGACCCTGAAAGCCAGTA+TGG + chr4.3:1679821-1679840 None:intergenic 50.0%
TGTTGCAGTCGCGGTAGAAT+TGG + chr4.3:1681307-1681326 None:intergenic 50.0%
TTCCGTAGATGTTGCAGTCG+CGG + chr4.3:1681316-1681335 None:intergenic 50.0%
! ACGACCTTTGAGAATACGGC+AGG - chr4.3:1681173-1681192 MS.gene063696:CDS 50.0%
! GGTAGCCTTTGAAGGCACAA+CGG + chr4.3:1681256-1681275 None:intergenic 50.0%
! GGTGTCTTGGTAGCCTTTGA+AGG + chr4.3:1681264-1681283 None:intergenic 50.0%
! GTGCTGTTACGGTGTTCTGT+TGG + chr4.3:1681415-1681434 None:intergenic 50.0%
! TGCAACAACACCCTCTGAGA+TGG + chr4.3:1679933-1679952 None:intergenic 50.0%
!! GTTGGTGGTGGTGCTAATAC+TGG - chr4.3:1681677-1681696 MS.gene063696:CDS 50.0%
!! TGCTTCAATGGCTTGGTCCA+TGG + chr4.3:1679426-1679445 None:intergenic 50.0%
AAGCTGCTTCAGGCAACACC+AGG - chr4.3:1679845-1679864 MS.gene063696:CDS 55.0%
ACAAGGTTGGGCTCCATGGA+TGG - chr4.3:1681610-1681629 MS.gene063696:CDS 55.0%
AGCTGCTTCAGGCAACACCA+GGG - chr4.3:1679846-1679865 MS.gene063696:CDS 55.0%
CAAGCAGTAGCTCTCCGTTC+CGG - chr4.3:1681209-1681228 MS.gene063696:CDS 55.0%
CAAGGTTGGGCTCCATGGAT+GGG - chr4.3:1681611-1681630 MS.gene063696:CDS 55.0%
GGGTCCTGCCGTATTCTCAA+AGG + chr4.3:1681180-1681199 None:intergenic 55.0%
TGCAGTCGCGGTAGAATTGG+CGG + chr4.3:1681304-1681323 None:intergenic 55.0%
TTCAGGCAACACCAGGGTCA+GGG - chr4.3:1679852-1679871 MS.gene063696:CDS 55.0%
! GGTAGACAGTGTGTTGGTGG+TGG + chr4.3:1679363-1679382 None:intergenic 55.0%
! GGTGGTGGTGCTAATACTGG+TGG - chr4.3:1681680-1681699 MS.gene063696:CDS 55.0%
!! AGACAGTGTGTTGGTGGTGG+TGG + chr4.3:1679360-1679379 None:intergenic 55.0%
!! AGTGGTGTGCCGTTTGATGC+AGG - chr4.3:1681803-1681822 MS.gene063696:CDS 55.0%
CGGAACGGAGAGCTACTGCT+TGG + chr4.3:1681211-1681230 None:intergenic 60.0%
CTTCAGGCAACACCAGGGTC+AGG - chr4.3:1679851-1679870 MS.gene063696:CDS 60.0%
GAAAACGGAGTGATCGGCGC+CGG + chr4.3:1681231-1681250 None:intergenic 60.0%
GTCGCGGTAGAATTGGCGGT+TGG + chr4.3:1681300-1681319 None:intergenic 60.0%
GGTTGGGCTCCATGGATGGG+TGG - chr4.3:1681614-1681633 MS.gene063696:CDS 65.0%
! CGGAGTGATCGGCGCCGGAA+CGG + chr4.3:1681226-1681245 None:intergenic 70.0%
!! CTTGGTGCTGGCCCTGACCC+TGG + chr4.3:1679866-1679885 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 1679206 1681832 1679206 ID=MS.gene063696
chr4.3 mRNA 1679206 1681832 1679206 ID=MS.gene063696.t1;Parent=MS.gene063696
chr4.3 exon 1680899 1681832 1680899 ID=MS.gene063696.t1.exon1;Parent=MS.gene063696.t1
chr4.3 CDS 1680899 1681832 1680899 ID=cds.MS.gene063696.t1;Parent=MS.gene063696.t1
chr4.3 exon 1679206 1679897 1679206 ID=MS.gene063696.t1.exon2;Parent=MS.gene063696.t1
chr4.3 CDS 1679206 1679897 1679206 ID=cds.MS.gene063696.t1;Parent=MS.gene063696.t1
Gene Sequence

>MS.gene063696

ATGCACAATATTGCATATCCTAATATGAATAAACTCATATTCAACTCTTGCATTGCATACTTTCTCCTTTTTTCTCTCTTGTTTGTGCATGGCAAAGAGTTATCATGCAATCAAACCCCATACCCTCATGTGTGTAACCATTACATTGGAACCACCACCACCAACACACTGTCTACCCTAGATTCTTCTTCTTTCCATGATATGGCTCTTAAGGTCACCATGGACCAAGCCATTGAAGCACACAAACTTGTATCAACCATGGAATTAAATAATTTCAAAGACAAGCGTGCCAAATCAGCATGGGAAGATTGTTTAGAGCTTTATGAGGATACAATTTATCAACTCAAACGCTCAATAAACTCAAACAACCTAAATGATAAATTAACTTGGCAAAGTGCTTCCATTACCAATCATCAAACTTGTCAAAATGGTTTCATTGATTTCAACCTTCCATCTCACTTAAACTACTTCCCATCCATGTTAAGCAACTTCACTAAATTGCTTAGCAATTCTTTGTCCATTAGCAACACTCTAACTTCATCACCATCATCCTCATCCACATCATCAAGTACTAAACAAAATGGAGGGAGAAGGTTGTTATCAGATAAATTTCCATACTGGCTTTCAGGGTCAGATAGAAAGCTGCTTCAGGCAACACCAGGGTCAGGGCCAGCACCAAGAGCTGATATTGTTGTTGCACAAGATGGAAGTGGAAACTATAAGACCATCTCAGAGGGTGTTGTTGCAGCTGCTAAGCTTAGTGGGAAAGGAAGAGTTGTCATTCATGTGAAATCAGGTGTTTATAGAGAGAATGTTGATATCAAGAAGACAGTGAAGAATTTAATGATATTTGGAGATGGAATGGACTCCACTATTGTTACTGGTAATCATAATGCTATAGATGGTTCTACTACTTTTCGTTCAGCTACTTTTGGTAAGTTAATCAATCCTTAATCACTAATCTATTCATATATCTATATATAGTTATATACTATAAATTATTATGTAAATAAATATCACAGTTGTTTTCTATATGCATGGGAGAAATCATATGATCAATGGAATAGTACGTAACAATAGTAGTAATATAAAAACAGTTTGAATTAAGTGAACACTAGAAAATATATCACTTTGGCCCCATTAAGATAGAGACATGCAGTACTTATTTATGTACAACTTGTGTTAAGGATCAAACATTATATTATATATTGAGTAACTACTTGTCATATGGCTATCAACAGATTAACAATAAAGTACAAATTTTTTAAATATCTTTTATCTTTGAAAACAGATAATATTTTCAAACTAAGTAATCTCTTTTTTTTTTGTCCCTACTTTATTTAACATTTGTTTTTAGTTACTTTTCTACTTTCTCTCAAAAGGTATTTTTTGTTCAATTTTTTTAATTAAAACATGTTGAAAACAAAAACAAAAATATTCTCTCAAAAAAAAAAAACAAAAATATAGCGAAGACATCATAACAATTATAAAACTGATATAAAGTCTAATCCATACGACCAACAATTATACCTTTTACATCAGTCTCAGAAAACATTGTGATTGAGATGTTTTTACTGTATAATAAAAGTGAAAATATCTCATTTCTATGTATTGACGTTGACTTTATTTTTTGTTTGTCAAATGATATATATTAATAAAACCTTTTTAACAATATTCACTAAAGTTCTGGAGTTTAATCTCCATGAACCGGTCAATTAATGTAAAAAGTTTTCAATTGTTCATTATTGTTTTACTTCACATATGAGTATCGTTCACAATCAATCATTTGCATGCCTTTAATAAAGTAATTATGACCACATACTATTAAATGTTCAGGCAATTATGATTGATCCATAGTGCAAAAAATTCTTTACATAAATATACTGTATGAATTAAATCTAAAGTTCTAACTATGAAATAAAATAATTATTGCAGCTGTTATGGGAGATGGTTTCATAGCTAAGGACACGACCTTTGAGAATACGGCAGGACCCCAAAAACACCAAGCAGTAGCTCTCCGTTCCGGCGCCGATCACTCCGTTTTCTACCGTTGTGCCTTCAAAGGCTACCAAGACACCTTATATGCTTACGCCAACCGCCAATTCTACCGCGACTGCAACATCTACGGAACTATTGATTTCATCTTCGGCAACGCAGTTACTGTCCTTCAAAACTGCAACATCTTCGTTAGAAAACCAATGAGCAACCAACAGAACACCGTAACAGCACAAGGAAGAACAGATCCAAATGAAAACACAGGAATTGTAATACATAACTGCCGTATAACCGCAGCAAATGATTTGAAACCGGTTCAGAATTCAGTTAAAACATATCTTGGTAGACCATGGCAGAAATATTCAAGAACGGTTGTAATGAAAAGTAATCTTGATGGTTTGATAAATTCACAAGGTTGGGCTCCATGGATGGGTGGTTTTGCATTGAGTACACTTTATTATGGTGAATATATGAATGTTGGTGGTGGTGCTAATACTGGTGGAAGAGTTAATTGGCCTGGTTTTCATGTTATTACTAATCCTTCTGAGGCTGTTAAATTTTCTGTTGGTAATTTTTTGGCTGGTGATTCTTGGATTTCAGGGAGTGGTGTGCCGTTTGATGCAGGTTTGTGA
Protein sequence

>MS.gene063696.t1

MHNIAYPNMNKLIFNSCIAYFLLFSLLFVHGKELSCNQTPYPHVCNHYIGTTTTNTLSTLDSSSFHDMALKVTMDQAIEAHKLVSTMELNNFKDKRAKSAWEDCLELYEDTIYQLKRSINSNNLNDKLTWQSASITNHQTCQNGFIDFNLPSHLNYFPSMLSNFTKLLSNSLSISNTLTSSPSSSSTSSSTKQNGGRRLLSDKFPYWLSGSDRKLLQATPGSGPAPRADIVVAQDGSGNYKTISEGVVAAAKLSGKGRVVIHVKSGVYRENVDIKKTVKNLMIFGDGMDSTIVTGNHNAIDGSTTFRSATFAVMGDGFIAKDTTFENTAGPQKHQAVALRSGADHSVFYRCAFKGYQDTLYAYANRQFYRDCNIYGTIDFIFGNAVTVLQNCNIFVRKPMSNQQNTVTAQGRTDPNENTGIVIHNCRITAANDLKPVQNSVKTYLGRPWQKYSRTVVMKSNLDGLINSQGWAPWMGGFALSTLYYGEYMNVGGGANTGGRVNWPGFHVITNPSEAVKFSVGNFLAGDSWISGSGVPFDAGL