Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049393.t1 | KEH35557.1 | 97.8 | 90 | 2 | 0 | 1 | 90 | 1 | 90 | 5.90E-41 | 176.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049393.t1 | A0A072V146 | 97.8 | 90 | 2 | 0 | 1 | 90 | 1 | 90 | 4.3e-41 | 176.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049393 | MS.gene06520 | 0.855811 | 4.91E-62 | -1.69E-46 |
| MS.gene049393 | MS.gene65502 | 0.801443 | 8.98E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049393.t1 | MTR_3g094120 | 97.778 | 90 | 2 | 0 | 1 | 90 | 1 | 90 | 1.71e-62 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTATGATTATGATTTCTAT+AGG | 0.366731 | 3.1:-75246651 | MS.gene049393:CDS |
| CCTCCACCTAGTGATGATTA+TGG | 0.382564 | 3.1:-75246673 | MS.gene049393:CDS |
| CGTGATGGATTACAGTGATC+AGG | 0.418617 | 3.1:-75246710 | MS.gene049393:CDS |
| GAGAGCAGTTGTACATCAAT+TGG | 0.422945 | 3.1:-75246757 | MS.gene049393:CDS |
| CAGTGACCAGCACCAGGACT+TGG | 0.469796 | 3.1:+75246613 | None:intergenic |
| TGCTTGTAGCTGCAATCTCT+TGG | 0.489828 | 3.1:+75246794 | None:intergenic |
| CACATCAAAGATAGAAGAAG+AGG | 0.533554 | 3.1:+75246817 | None:intergenic |
| TAATCATCACTAGGTGGAGG+TGG | 0.538814 | 3.1:+75246676 | None:intergenic |
| CATGGTGATATTCCAAGTCC+TGG | 0.543326 | 3.1:-75246625 | MS.gene049393:CDS |
| CTTTATCAGTGACCAGCACC+AGG | 0.558843 | 3.1:+75246607 | None:intergenic |
| GATATTCCAAGTCCTGGTGC+TGG | 0.576712 | 3.1:-75246619 | MS.gene049393:CDS |
| CCATGTCAAAGTCACCGTGA+TGG | 0.583602 | 3.1:-75246725 | MS.gene049393:CDS |
| CCATAATCATCACTAGGTGG+AGG | 0.607825 | 3.1:+75246673 | None:intergenic |
| GTGATGGATTACAGTGATCA+GGG | 0.608820 | 3.1:-75246709 | MS.gene049393:CDS |
| TATGATTTCTATAGGAAACA+TGG | 0.610105 | 3.1:-75246643 | MS.gene049393:CDS |
| TCATAGCCATAATCATCACT+AGG | 0.627153 | 3.1:+75246667 | None:intergenic |
| CCATCACGGTGACTTTGACA+TGG | 0.629958 | 3.1:+75246725 | None:intergenic |
| CTGATCACTGTAATCCATCA+CGG | 0.656835 | 3.1:+75246711 | None:intergenic |
| TAGCCATAATCATCACTAGG+TGG | 0.699004 | 3.1:+75246670 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GCTATGATTATGATTTCTAT+AGG | - | chr3.1:75246823-75246842 | MS.gene049393:CDS | 25.0% |
| ! | TATGATTTCTATAGGAAACA+TGG | - | chr3.1:75246831-75246850 | MS.gene049393:CDS | 25.0% |
| CACATCAAAGATAGAAGAAG+AGG | + | chr3.1:75246660-75246679 | None:intergenic | 35.0% | |
| TCATAGCCATAATCATCACT+AGG | + | chr3.1:75246810-75246829 | None:intergenic | 35.0% | |
| CTGATCACTGTAATCCATCA+CGG | + | chr3.1:75246766-75246785 | None:intergenic | 40.0% | |
| GAGAGCAGTTGTACATCAAT+TGG | - | chr3.1:75246717-75246736 | MS.gene049393:CDS | 40.0% | |
| TAGCCATAATCATCACTAGG+TGG | + | chr3.1:75246807-75246826 | None:intergenic | 40.0% | |
| !! | GTGATGGATTACAGTGATCA+GGG | - | chr3.1:75246765-75246784 | MS.gene049393:CDS | 40.0% |
| CATGGTGATATTCCAAGTCC+TGG | - | chr3.1:75246849-75246868 | MS.gene049393:CDS | 45.0% | |
| CCATAATCATCACTAGGTGG+AGG | + | chr3.1:75246804-75246823 | None:intergenic | 45.0% | |
| CCTCCACCTAGTGATGATTA+TGG | - | chr3.1:75246801-75246820 | MS.gene049393:CDS | 45.0% | |
| TAATCATCACTAGGTGGAGG+TGG | + | chr3.1:75246801-75246820 | None:intergenic | 45.0% | |
| TGCTTGTAGCTGCAATCTCT+TGG | + | chr3.1:75246683-75246702 | None:intergenic | 45.0% | |
| !! | CGTGATGGATTACAGTGATC+AGG | - | chr3.1:75246764-75246783 | MS.gene049393:CDS | 45.0% |
| CCATCACGGTGACTTTGACA+TGG | + | chr3.1:75246752-75246771 | None:intergenic | 50.0% | |
| CCATGTCAAAGTCACCGTGA+TGG | - | chr3.1:75246749-75246768 | MS.gene049393:CDS | 50.0% | |
| GATATTCCAAGTCCTGGTGC+TGG | - | chr3.1:75246855-75246874 | MS.gene049393:CDS | 50.0% | |
| CAGTGACCAGCACCAGGACT+TGG | + | chr3.1:75246864-75246883 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 75246612 | 75246884 | 75246612 | ID=MS.gene049393 |
| chr3.1 | mRNA | 75246612 | 75246884 | 75246612 | ID=MS.gene049393.t1;Parent=MS.gene049393 |
| chr3.1 | exon | 75246612 | 75246884 | 75246612 | ID=MS.gene049393.t1.exon1;Parent=MS.gene049393.t1 |
| chr3.1 | CDS | 75246612 | 75246884 | 75246612 | ID=cds.MS.gene049393.t1;Parent=MS.gene049393.t1 |
| Gene Sequence |
| Protein sequence |
