Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06520.t1 | KEH35557.1 | 97.8 | 90 | 2 | 0 | 1 | 90 | 1 | 90 | 5.90E-41 | 176.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06520.t1 | A0A072V146 | 97.8 | 90 | 2 | 0 | 1 | 90 | 1 | 90 | 4.3e-41 | 176.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene06520 | -0.802089 | 6.62E-49 | -1.69E-46 |
| MS.gene049393 | MS.gene06520 | 0.855811 | 4.91E-62 | -1.69E-46 |
| MS.gene050082 | MS.gene06520 | 0.804132 | 2.50E-49 | -1.69E-46 |
| MS.gene050338 | MS.gene06520 | 0.811082 | 8.36E-51 | -1.69E-46 |
| MS.gene050444 | MS.gene06520 | -0.815972 | 7.03E-52 | -1.69E-46 |
| MS.gene050612 | MS.gene06520 | -0.807458 | 5.00E-50 | -1.69E-46 |
| MS.gene050876 | MS.gene06520 | -0.817572 | 3.08E-52 | -1.69E-46 |
| MS.gene050904 | MS.gene06520 | -0.852152 | 5.57E-61 | -1.69E-46 |
| MS.gene051317 | MS.gene06520 | -0.806666 | 7.36E-50 | -1.69E-46 |
| MS.gene051364 | MS.gene06520 | 0.80072 | 1.26E-48 | -1.69E-46 |
| MS.gene051366 | MS.gene06520 | 0.806695 | 7.26E-50 | -1.69E-46 |
| MS.gene051367 | MS.gene06520 | 0.800909 | 1.16E-48 | -1.69E-46 |
| MS.gene051651 | MS.gene06520 | 0.809443 | 1.89E-50 | -1.69E-46 |
| MS.gene053776 | MS.gene06520 | 0.817666 | 2.93E-52 | -1.69E-46 |
| MS.gene055158 | MS.gene06520 | 0.82236 | 2.47E-53 | -1.69E-46 |
| MS.gene055748 | MS.gene06520 | -0.823133 | 1.63E-53 | -1.69E-46 |
| MS.gene055750 | MS.gene06520 | -0.804532 | 2.07E-49 | -1.69E-46 |
| MS.gene055751 | MS.gene06520 | -0.830456 | 2.91E-55 | -1.69E-46 |
| MS.gene05589 | MS.gene06520 | -0.822773 | 1.98E-53 | -1.69E-46 |
| MS.gene056387 | MS.gene06520 | -0.801164 | 1.02E-48 | -1.69E-46 |
| MS.gene056504 | MS.gene06520 | -0.807912 | 4.01E-50 | -1.69E-46 |
| MS.gene056618 | MS.gene06520 | 0.809516 | 1.82E-50 | -1.69E-46 |
| MS.gene056856 | MS.gene06520 | 0.813998 | 1.93E-51 | -1.69E-46 |
| MS.gene058773 | MS.gene06520 | -0.802772 | 4.79E-49 | -1.69E-46 |
| MS.gene059079 | MS.gene06520 | -0.810166 | 1.32E-50 | -1.69E-46 |
| MS.gene059526 | MS.gene06520 | 0.827809 | 1.27E-54 | -1.69E-46 |
| MS.gene059865 | MS.gene06520 | -0.807202 | 5.67E-50 | -1.69E-46 |
| MS.gene06000 | MS.gene06520 | -0.80566 | 1.20E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene06520 | -0.83692 | 7.05E-57 | -1.69E-46 |
| MS.gene060823 | MS.gene06520 | 0.841475 | 4.65E-58 | -1.69E-46 |
| MS.gene060982 | MS.gene06520 | -0.809715 | 1.65E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06520.t1 | MTR_3g094120 | 97.778 | 90 | 2 | 0 | 1 | 90 | 1 | 90 | 1.71e-62 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTATGATTATGATTTCTAT+AGG | 0.366731 | 3.2:-78903236 | MS.gene06520:CDS |
| CCTCCACCTAGTGATGATTA+TGG | 0.382564 | 3.2:-78903258 | MS.gene06520:CDS |
| CGTGATGGATTACAGTGATC+AGG | 0.418617 | 3.2:-78903295 | MS.gene06520:CDS |
| GAGAGCAGTTGTACATCAAT+TGG | 0.422945 | 3.2:-78903342 | MS.gene06520:CDS |
| CAGTGACCAGCACCAGGACT+TGG | 0.482976 | 3.2:+78903198 | None:intergenic |
| TGCTTGTAGCTGCAATCTCT+TGG | 0.489828 | 3.2:+78903379 | None:intergenic |
| CACATCAAAGATAGAAGAAG+AGG | 0.533554 | 3.2:+78903402 | None:intergenic |
| TAATCATCACTAGGTGGAGG+TGG | 0.538814 | 3.2:+78903261 | None:intergenic |
| CATGGTGATATTCCAAGTCC+TGG | 0.543326 | 3.2:-78903210 | MS.gene06520:CDS |
| CTTCATCAGTGACCAGCACC+AGG | 0.558843 | 3.2:+78903192 | None:intergenic |
| GATATTCCAAGTCCTGGTGC+TGG | 0.576712 | 3.2:-78903204 | MS.gene06520:CDS |
| CCATGTCAAAGTCACCGTGA+TGG | 0.583602 | 3.2:-78903310 | MS.gene06520:CDS |
| CCATAATCATCACTAGGTGG+AGG | 0.607825 | 3.2:+78903258 | None:intergenic |
| GTGATGGATTACAGTGATCA+GGG | 0.608820 | 3.2:-78903294 | MS.gene06520:CDS |
| TATGATTTCTATAGGAAACA+TGG | 0.610105 | 3.2:-78903228 | MS.gene06520:CDS |
| TCATAGCCATAATCATCACT+AGG | 0.627153 | 3.2:+78903252 | None:intergenic |
| CCATCACGGTGACTTTGACA+TGG | 0.629958 | 3.2:+78903310 | None:intergenic |
| CTGATCACTGTAATCCATCA+CGG | 0.656835 | 3.2:+78903296 | None:intergenic |
| TAGCCATAATCATCACTAGG+TGG | 0.699004 | 3.2:+78903255 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GCTATGATTATGATTTCTAT+AGG | - | chr3.2:78903408-78903427 | MS.gene06520:CDS | 25.0% |
| ! | TATGATTTCTATAGGAAACA+TGG | - | chr3.2:78903416-78903435 | MS.gene06520:CDS | 25.0% |
| CACATCAAAGATAGAAGAAG+AGG | + | chr3.2:78903245-78903264 | None:intergenic | 35.0% | |
| TCATAGCCATAATCATCACT+AGG | + | chr3.2:78903395-78903414 | None:intergenic | 35.0% | |
| CTGATCACTGTAATCCATCA+CGG | + | chr3.2:78903351-78903370 | None:intergenic | 40.0% | |
| GAGAGCAGTTGTACATCAAT+TGG | - | chr3.2:78903302-78903321 | MS.gene06520:CDS | 40.0% | |
| TAGCCATAATCATCACTAGG+TGG | + | chr3.2:78903392-78903411 | None:intergenic | 40.0% | |
| !! | GTGATGGATTACAGTGATCA+GGG | - | chr3.2:78903350-78903369 | MS.gene06520:CDS | 40.0% |
| CATGGTGATATTCCAAGTCC+TGG | - | chr3.2:78903434-78903453 | MS.gene06520:CDS | 45.0% | |
| CCATAATCATCACTAGGTGG+AGG | + | chr3.2:78903389-78903408 | None:intergenic | 45.0% | |
| CCTCCACCTAGTGATGATTA+TGG | - | chr3.2:78903386-78903405 | MS.gene06520:CDS | 45.0% | |
| TAATCATCACTAGGTGGAGG+TGG | + | chr3.2:78903386-78903405 | None:intergenic | 45.0% | |
| TGCTTGTAGCTGCAATCTCT+TGG | + | chr3.2:78903268-78903287 | None:intergenic | 45.0% | |
| !! | CGTGATGGATTACAGTGATC+AGG | - | chr3.2:78903349-78903368 | MS.gene06520:CDS | 45.0% |
| CCATCACGGTGACTTTGACA+TGG | + | chr3.2:78903337-78903356 | None:intergenic | 50.0% | |
| CCATGTCAAAGTCACCGTGA+TGG | - | chr3.2:78903334-78903353 | MS.gene06520:CDS | 50.0% | |
| GATATTCCAAGTCCTGGTGC+TGG | - | chr3.2:78903440-78903459 | MS.gene06520:CDS | 50.0% | |
| CAGTGACCAGCACCAGGACT+TGG | + | chr3.2:78903449-78903468 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 78903197 | 78903469 | 78903197 | ID=MS.gene06520 |
| chr3.2 | mRNA | 78903197 | 78903469 | 78903197 | ID=MS.gene06520.t1;Parent=MS.gene06520 |
| chr3.2 | exon | 78903197 | 78903469 | 78903197 | ID=MS.gene06520.t1.exon1;Parent=MS.gene06520.t1 |
| chr3.2 | CDS | 78903197 | 78903469 | 78903197 | ID=cds.MS.gene06520.t1;Parent=MS.gene06520.t1 |
| Gene Sequence |
| Protein sequence |
