AlfalfaGEDB | MS.gene65502

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65502.t1	RHN78606.1	80.4	102	6	1	1	88	13	114	9.00E-26	125.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65502.t1	G7I5D6	80.4	102	6	1	1	88	1	102	6.5e-26	125.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049393	MS.gene65502	0.801443	8.98E-49	-1.69E-46
MS.gene050082	MS.gene65502	0.868056	8.67E-66	-1.69E-46
MS.gene051138	MS.gene65502	0.800849	1.19E-48	-1.69E-46
MS.gene051142	MS.gene65502	0.820006	8.61E-53	-1.69E-46
MS.gene054057	MS.gene65502	0.819009	1.45E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65502.t1	MTR_1g043320	72.549	102	14	1	1	88	1	102	1.47e-26	94.0

Find 22 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGGTGGCCAGTGTTTCTT+AGG	0.235134	1.4:+27992409	None:intergenic
GGGATTGTTTGGTCCACTTT+TGG	0.317502	1.4:+27992346	None:intergenic
GTAGGAGGCCAGTGTTTCTT+TGG	0.327069	1.4:+27992391	None:intergenic
TGGAAGAAGCATCCTCCTTC+AGG	0.366754	1.4:-27992253	MS.gene65502:CDS
ATCTCACTGTGTCTTCTACT+AGG	0.408144	1.4:-27992475	MS.gene65502:CDS
GAAGGAGGATGCTTCTTCCA+TGG	0.436291	1.4:+27992256	None:intergenic
GGAGGCCAGTGTTTCTTTGG+TGG	0.468858	1.4:+27992394	None:intergenic
ACAAAATGACCTTCCAAAAG+TGG	0.469837	1.4:-27992359	MS.gene65502:CDS
AAGGAGGATGCTTCTTCCAT+GGG	0.483462	1.4:+27992257	None:intergenic
GCTTCTTCCATGGGTGCTTG+TGG	0.496634	1.4:+27992266	None:intergenic
TCTCACTGTGTCTTCTACTA+GGG	0.525074	1.4:-27992474	MS.gene65502:CDS
GGTGGCCAGTGTTTCTTAGG+TGG	0.530733	1.4:+27992412	None:intergenic
AGCATCACCACAAGCACCCA+TGG	0.549276	1.4:-27992273	MS.gene65502:CDS
ATTAATCAATTTCCTGAAGG+AGG	0.566620	1.4:+27992241	None:intergenic
ATGATTAATCAATTTCCTGA+AGG	0.570240	1.4:+27992238	None:intergenic
ACAAAACATACAAGCACAAG+AGG	0.577700	1.4:-27992323	MS.gene65502:CDS
ACTGGCCACCAAAGAAACAC+TGG	0.593745	1.4:-27992399	MS.gene65502:CDS
GACTATCCTCAGCTATAGTG+TGG	0.603968	1.4:+27992299	None:intergenic
ATGATCTGCTAGAGAAGTGG+TGG	0.618374	1.4:+27992438	None:intergenic
ATAAGCCACCTAAGAAACAC+TGG	0.623404	1.4:-27992417	MS.gene65502:CDS
CTTATGATCTGCTAGAGAAG+TGG	0.629676	1.4:+27992435	None:intergenic
AGAGGACCACACTATAGCTG+AGG	0.685652	1.4:-27992305	MS.gene65502:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ACAAAACATACAAGCACAAG+AGG	-	chr1.4:27992413-27992432	MS.gene65502:CDS	35.0%
	ACAAAATGACCTTCCAAAAG+TGG	-	chr1.4:27992377-27992396	MS.gene65502:CDS	35.0%
!	CTTGTGCTTGTATGTTTTGT+GGG	+	chr1.4:27992413-27992432	None:intergenic	35.0%
!	GTATGTTTTGTGGGATTGTT+TGG	+	chr1.4:27992404-27992423	None:intergenic	35.0%
!	TCATTTTGTGTAGTTGTGGT+AGG	+	chr1.4:27992366-27992385	None:intergenic	35.0%
!	TCTTGTGCTTGTATGTTTTG+TGG	+	chr1.4:27992414-27992433	None:intergenic	35.0%
!!	AAGGTCATTTTGTGTAGTTG+TGG	+	chr1.4:27992370-27992389	None:intergenic	35.0%
	ATAAGCCACCTAAGAAACAC+TGG	-	chr1.4:27992319-27992338	MS.gene65502:CDS	40.0%
	ATCTCACTGTGTCTTCTACT+AGG	-	chr1.4:27992261-27992280	MS.gene65502:CDS	40.0%
	CTTATGATCTGCTAGAGAAG+TGG	+	chr1.4:27992304-27992323	None:intergenic	40.0%
	TCTCACTGTGTCTTCTACTA+GGG	-	chr1.4:27992262-27992281	MS.gene65502:CDS	40.0%
!	TTTTGTGTAGTTGTGGTAGG+AGG	+	chr1.4:27992363-27992382	None:intergenic	40.0%
!!	TTGTTTGGTCCACTTTTGGA+AGG	+	chr1.4:27992389-27992408	None:intergenic	40.0%
	AAGGAGGATGCTTCTTCCAT+GGG	+	chr1.4:27992482-27992501	None:intergenic	45.0%
	ATGATCTGCTAGAGAAGTGG+TGG	+	chr1.4:27992301-27992320	None:intergenic	45.0%
	GACTATCCTCAGCTATAGTG+TGG	+	chr1.4:27992440-27992459	None:intergenic	45.0%
!	GGGATTGTTTGGTCCACTTT+TGG	+	chr1.4:27992393-27992412	None:intergenic	45.0%
!	TTTGGTGGCCAGTGTTTCTT+AGG	+	chr1.4:27992330-27992349	None:intergenic	45.0%
	ACTGGCCACCAAAGAAACAC+TGG	-	chr1.4:27992337-27992356	MS.gene65502:CDS	50.0%
	AGAGGACCACACTATAGCTG+AGG	-	chr1.4:27992431-27992450	MS.gene65502:CDS	50.0%
	GAAGGAGGATGCTTCTTCCA+TGG	+	chr1.4:27992483-27992502	None:intergenic	50.0%
	GTAGGAGGCCAGTGTTTCTT+TGG	+	chr1.4:27992348-27992367	None:intergenic	50.0%
!!	TGGAAGAAGCATCCTCCTTC+AGG	-	chr1.4:27992483-27992502	MS.gene65502:CDS	50.0%
	AGCATCACCACAAGCACCCA+TGG	-	chr1.4:27992463-27992482	MS.gene65502:CDS	55.0%
	GCTTCTTCCATGGGTGCTTG+TGG	+	chr1.4:27992473-27992492	None:intergenic	55.0%
	GGAGGCCAGTGTTTCTTTGG+TGG	+	chr1.4:27992345-27992364	None:intergenic	55.0%
	GGTGGCCAGTGTTTCTTAGG+TGG	+	chr1.4:27992327-27992346	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	27992246	27992512	27992246	ID=MS.gene65502
chr1.4	mRNA	27992246	27992512	27992246	ID=MS.gene65502.t1;Parent=MS.gene65502
chr1.4	exon	27992246	27992512	27992246	ID=MS.gene65502.t1.exon1;Parent=MS.gene65502.t1
chr1.4	CDS	27992246	27992512	27992246	ID=cds.MS.gene65502.t1;Parent=MS.gene65502.t1

Gene Sequence	>MS.gene65502 ATGTCTCTCAAATACATCTCACTGTGTCTTCTACTAGGGATAGTGCTTCTTGCCACCACTTCTCTAGCAGATCATAAGCCACCTAAGAAACACTGGCCACCAAAGAAACACTGGCCTCCTACCACAACTACACAAAATGACCTTCCAAAAGTGGACCAAACAATCCCACAAAACATACAAGCACAAGAGGACCACACTATAGCTGAGGATAGTCACAAGCATCACCACAAGCACCCATGGAAGAAGCATCCTCCTTCAGGAAATTGA
Protein sequence	>MS.gene65502.t1 MSLKYISLCLLLGIVLLATTSLADHKPPKKHWPPKKHWPPTTTTQNDLPKVDQTIPQNIQAQEDHTIAEDSHKHHHKHPWKKHPPSGN

M. sativa cultivar XinJiangDaYe / MS.gene65502