Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049712.t1 | XP_013444920.1 | 89.2 | 102 | 11 | 0 | 1 | 102 | 64 | 165 | 2.90E-45 | 191 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049712.t1 | A0A072TZI9 | 89.2 | 102 | 11 | 0 | 1 | 102 | 64 | 165 | 2.1e-45 | 191.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049712 | MS.gene36870 | 0.802334 | 5.89E-49 | -1.69E-46 |
| MS.gene049712 | MS.gene58300 | 0.803074 | 4.15E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049712.t1 | MTR_8g432490 | 89.216 | 102 | 11 | 0 | 1 | 102 | 64 | 165 | 2.29e-63 | 191 |
| MS.gene049712.t1 | MTR_8g432460 | 86.275 | 102 | 14 | 0 | 1 | 102 | 64 | 165 | 2.27e-61 | 185 |
| MS.gene049712.t1 | MTR_3g064780 | 85.294 | 102 | 15 | 0 | 1 | 102 | 64 | 165 | 1.10e-58 | 181 |
| MS.gene049712.t1 | MTR_8g432440 | 77.670 | 103 | 22 | 1 | 1 | 102 | 64 | 166 | 4.93e-53 | 164 |
| MS.gene049712.t1 | MTR_8g432420 | 77.670 | 103 | 22 | 1 | 1 | 102 | 64 | 166 | 4.93e-53 | 164 |
| MS.gene049712.t1 | MTR_8g432470 | 78.431 | 102 | 22 | 0 | 1 | 102 | 65 | 166 | 5.15e-53 | 164 |
| MS.gene049712.t1 | MTR_8g432450 | 76.699 | 103 | 23 | 1 | 1 | 102 | 65 | 167 | 4.62e-51 | 159 |
| MS.gene049712.t1 | MTR_8g432480 | 51.852 | 81 | 38 | 1 | 1 | 80 | 65 | 145 | 2.39e-23 | 89.0 |
| MS.gene049712.t1 | MTR_8g432370 | 44.776 | 67 | 37 | 0 | 1 | 67 | 62 | 128 | 2.00e-12 | 60.5 |
| MS.gene049712.t1 | MTR_8g432400 | 47.143 | 70 | 36 | 1 | 1 | 69 | 62 | 131 | 2.04e-12 | 60.5 |
| MS.gene049712.t1 | MTR_8g432350 | 45.588 | 68 | 36 | 1 | 1 | 67 | 62 | 129 | 5.36e-12 | 59.3 |
| MS.gene049712.t1 | MTR_8g432390 | 45.588 | 68 | 36 | 1 | 1 | 67 | 62 | 129 | 3.65e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATGGAGTTATTCATTT+TGG | 0.193044 | 3.3:+58419464 | MS.gene049712:CDS |
| TTCAAAAGTGTTCGCCCTAT+TGG | 0.292098 | 3.3:+58419344 | MS.gene049712:CDS |
| GGGATTCTTGCCACCCCAAT+AGG | 0.301800 | 3.3:-58419359 | None:intergenic |
| TCAAAAGTGTTCGCCCTATT+GGG | 0.316840 | 3.3:+58419345 | MS.gene049712:CDS |
| TATTCTAAACGGGGCTGAAT+TGG | 0.327706 | 3.3:+58419391 | MS.gene049712:CDS |
| AGTTATTGTGATCGGTTAAC+TGG | 0.349361 | 3.3:+58419557 | MS.gene049712:CDS |
| GATTGGGAAGGGAAGCAAAA+TGG | 0.374296 | 3.3:+58419449 | MS.gene049712:CDS |
| CCCGTTTCGGATATTCTAAA+CGG | 0.390478 | 3.3:+58419380 | MS.gene049712:CDS |
| GAACTATTGGTGTTCCTGTT+TGG | 0.405823 | 3.3:+58419598 | MS.gene049712:CDS |
| TGAGTTATAAACTGAGGGAT+TGG | 0.418159 | 3.3:+58419432 | MS.gene049712:CDS |
| ATTGGCTACCGTGGCGGAAA+AGG | 0.420371 | 3.3:+58419514 | MS.gene049712:CDS |
| CCGTTTCGGATATTCTAAAC+GGG | 0.420732 | 3.3:+58419381 | MS.gene049712:CDS |
| ACAGGAACACCAATAGTTCT+TGG | 0.423198 | 3.3:-58419594 | None:intergenic |
| CCGTTTAGAATATCCGAAAC+GGG | 0.434294 | 3.3:-58419380 | None:intergenic |
| TCAGTTTATAACTCAACATC+CGG | 0.444784 | 3.3:-58419424 | None:intergenic |
| GAGTTATAAACTGAGGGATT+GGG | 0.449638 | 3.3:+58419433 | MS.gene049712:CDS |
| CTGAATTGGAAAATTGTTCC+CGG | 0.456171 | 3.3:+58419405 | MS.gene049712:CDS |
| GGTGGCAAGAATCCCCGTTT+CGG | 0.458489 | 3.3:+58419367 | MS.gene049712:CDS |
| GGATTCTTGCCACCCCAATA+GGG | 0.475826 | 3.3:-58419358 | None:intergenic |
| CTAGGGTAATTCCACCAAAC+AGG | 0.484703 | 3.3:-58419612 | None:intergenic |
| CCCGTTTAGAATATCCGAAA+CGG | 0.512439 | 3.3:-58419381 | None:intergenic |
| GGTTATGCCGGAGAAAAGAT+TGG | 0.515464 | 3.3:+58419496 | MS.gene049712:CDS |
| CTATTGGTGTTCCTGTTTGG+TGG | 0.518353 | 3.3:+58419601 | MS.gene049712:CDS |
| ATGTTGGTACCAAGAACTAT+TGG | 0.518426 | 3.3:+58419585 | MS.gene049712:CDS |
| CGGTTAACTGGTATTGATGT+TGG | 0.523272 | 3.3:+58419569 | MS.gene049712:CDS |
| TGGTGTGAGGGTGGTTATGC+CGG | 0.526142 | 3.3:+58419484 | MS.gene049712:CDS |
| TGCAAGGTCAATCACATTTG+AGG | 0.534627 | 3.3:+58419307 | MS.gene049712:CDS |
| TGAACATGCCATTGCATGCA+AGG | 0.566598 | 3.3:+58419291 | None:intergenic |
| CAAAAGTGTTCGCCCTATTG+GGG | 0.566660 | 3.3:+58419346 | MS.gene049712:CDS |
| ATAAACTGAGGGATTGGGAA+GGG | 0.569199 | 3.3:+58419438 | MS.gene049712:CDS |
| GATGTTGAGTTATAAACTGA+GGG | 0.570650 | 3.3:+58419427 | MS.gene049712:CDS |
| ATGGAAAGAGTTATTGTGAT+CGG | 0.575040 | 3.3:+58419549 | MS.gene049712:CDS |
| GTGATTGACCTTGCATGCAA+TGG | 0.597541 | 3.3:-58419299 | None:intergenic |
| GAAAAGGAAACAATGGCGGA+TGG | 0.603995 | 3.3:+58419530 | MS.gene049712:CDS |
| TATAAACTGAGGGATTGGGA+AGG | 0.629947 | 3.3:+58419437 | MS.gene049712:CDS |
| AAGTGTTCGCCCTATTGGGG+TGG | 0.646267 | 3.3:+58419349 | MS.gene049712:CDS |
| GGAGAAAAGATTGGCTACCG+TGG | 0.651552 | 3.3:+58419505 | MS.gene049712:CDS |
| CGTTTCGGATATTCTAAACG+GGG | 0.653561 | 3.3:+58419382 | MS.gene049712:CDS |
| CAGTTTATAACTCAACATCC+GGG | 0.658129 | 3.3:-58419423 | None:intergenic |
| CGTTTAGAATATCCGAAACG+GGG | 0.658786 | 3.3:-58419379 | None:intergenic |
| CGTGGCGGAAAAGGAAACAA+TGG | 0.664549 | 3.3:+58419523 | MS.gene049712:CDS |
| GGATGTTGAGTTATAAACTG+AGG | 0.678004 | 3.3:+58419426 | MS.gene049712:CDS |
| GAAAAGATTGGCTACCGTGG+CGG | 0.683791 | 3.3:+58419508 | MS.gene049712:CDS |
| GGCGGAAAAGGAAACAATGG+CGG | 0.701876 | 3.3:+58419526 | MS.gene049712:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAAATGGAGTTATTCATTT+TGG | + | chr3.3:58419464-58419483 | MS.gene049712:CDS | 25.0% |
| ATGGAAAGAGTTATTGTGAT+CGG | + | chr3.3:58419549-58419568 | MS.gene049712:CDS | 30.0% | |
| GATGTTGAGTTATAAACTGA+GGG | + | chr3.3:58419427-58419446 | MS.gene049712:CDS | 30.0% | |
| TCAGTTTATAACTCAACATC+CGG | - | chr3.3:58419427-58419446 | None:intergenic | 30.0% | |
| !! | AGTTATTCATTTTGGTGTGA+GGG | + | chr3.3:58419472-58419491 | MS.gene049712:CDS | 30.0% |
| AGTTATTGTGATCGGTTAAC+TGG | + | chr3.3:58419557-58419576 | MS.gene049712:CDS | 35.0% | |
| ATGTTGGTACCAAGAACTAT+TGG | + | chr3.3:58419585-58419604 | MS.gene049712:CDS | 35.0% | |
| CAGTTTATAACTCAACATCC+GGG | - | chr3.3:58419426-58419445 | None:intergenic | 35.0% | |
| CTGAATTGGAAAATTGTTCC+CGG | + | chr3.3:58419405-58419424 | MS.gene049712:CDS | 35.0% | |
| GAGTTATAAACTGAGGGATT+GGG | + | chr3.3:58419433-58419452 | MS.gene049712:CDS | 35.0% | |
| GGATGTTGAGTTATAAACTG+AGG | + | chr3.3:58419426-58419445 | MS.gene049712:CDS | 35.0% | |
| TGAGTTATAAACTGAGGGAT+TGG | + | chr3.3:58419432-58419451 | MS.gene049712:CDS | 35.0% | |
| !! | GAGTTATTCATTTTGGTGTG+AGG | + | chr3.3:58419471-58419490 | MS.gene049712:CDS | 35.0% |
| ACAGGAACACCAATAGTTCT+TGG | - | chr3.3:58419597-58419616 | None:intergenic | 40.0% | |
| ATAAACTGAGGGATTGGGAA+GGG | + | chr3.3:58419438-58419457 | MS.gene049712:CDS | 40.0% | |
| CCCGTTTCGGATATTCTAAA+CGG | + | chr3.3:58419380-58419399 | MS.gene049712:CDS | 40.0% | |
| CCGTTTCGGATATTCTAAAC+GGG | + | chr3.3:58419381-58419400 | MS.gene049712:CDS | 40.0% | |
| CGTTTCGGATATTCTAAACG+GGG | + | chr3.3:58419382-58419401 | MS.gene049712:CDS | 40.0% | |
| TATAAACTGAGGGATTGGGA+AGG | + | chr3.3:58419437-58419456 | MS.gene049712:CDS | 40.0% | |
| TATTCTAAACGGGGCTGAAT+TGG | + | chr3.3:58419391-58419410 | MS.gene049712:CDS | 40.0% | |
| TGCAAGGTCAATCACATTTG+AGG | + | chr3.3:58419307-58419326 | MS.gene049712:CDS | 40.0% | |
| ! | CCCGTTTAGAATATCCGAAA+CGG | - | chr3.3:58419384-58419403 | None:intergenic | 40.0% |
| ! | CCGTTTAGAATATCCGAAAC+GGG | - | chr3.3:58419383-58419402 | None:intergenic | 40.0% |
| ! | CGTTTAGAATATCCGAAACG+GGG | - | chr3.3:58419382-58419401 | None:intergenic | 40.0% |
| ! | GAACTATTGGTGTTCCTGTT+TGG | + | chr3.3:58419598-58419617 | MS.gene049712:CDS | 40.0% |
| !! | CGGTTAACTGGTATTGATGT+TGG | + | chr3.3:58419569-58419588 | MS.gene049712:CDS | 40.0% |
| !! | TATTCATTTTGGTGTGAGGG+TGG | + | chr3.3:58419475-58419494 | MS.gene049712:CDS | 40.0% |
| !! | TCAAAAGTGTTCGCCCTATT+GGG | + | chr3.3:58419345-58419364 | MS.gene049712:CDS | 40.0% |
| !! | TTCAAAAGTGTTCGCCCTAT+TGG | + | chr3.3:58419344-58419363 | MS.gene049712:CDS | 40.0% |
| CTAGGGTAATTCCACCAAAC+AGG | - | chr3.3:58419615-58419634 | None:intergenic | 45.0% | |
| GATTGGGAAGGGAAGCAAAA+TGG | + | chr3.3:58419449-58419468 | MS.gene049712:CDS | 45.0% | |
| GGTTATGCCGGAGAAAAGAT+TGG | + | chr3.3:58419496-58419515 | MS.gene049712:CDS | 45.0% | |
| GTGATTGACCTTGCATGCAA+TGG | - | chr3.3:58419302-58419321 | None:intergenic | 45.0% | |
| ! | ACGGTAGCCAATCTTTTCTC+CGG | - | chr3.3:58419506-58419525 | None:intergenic | 45.0% |
| ! | CATTGTTTCCTTTTCCGCCA+CGG | - | chr3.3:58419525-58419544 | None:intergenic | 45.0% |
| ! | CTATTGGTGTTCCTGTTTGG+TGG | + | chr3.3:58419601-58419620 | MS.gene049712:CDS | 45.0% |
| ! | GAAAAGGAAACAATGGCGGA+TGG | + | chr3.3:58419530-58419549 | MS.gene049712:CDS | 45.0% |
| !! | CAAAAGTGTTCGCCCTATTG+GGG | + | chr3.3:58419346-58419365 | MS.gene049712:CDS | 45.0% |
| GGATTCTTGCCACCCCAATA+GGG | - | chr3.3:58419361-58419380 | None:intergenic | 50.0% | |
| GGCGGAAAAGGAAACAATGG+CGG | + | chr3.3:58419526-58419545 | MS.gene049712:CDS | 50.0% | |
| ! | CGTGGCGGAAAAGGAAACAA+TGG | + | chr3.3:58419523-58419542 | MS.gene049712:CDS | 50.0% |
| ! | GAAAAGATTGGCTACCGTGG+CGG | + | chr3.3:58419508-58419527 | MS.gene049712:CDS | 50.0% |
| ! | GGAGAAAAGATTGGCTACCG+TGG | + | chr3.3:58419505-58419524 | MS.gene049712:CDS | 50.0% |
| AAGTGTTCGCCCTATTGGGG+TGG | + | chr3.3:58419349-58419368 | MS.gene049712:CDS | 55.0% | |
| GGGATTCTTGCCACCCCAAT+AGG | - | chr3.3:58419362-58419381 | None:intergenic | 55.0% | |
| GGTGGCAAGAATCCCCGTTT+CGG | + | chr3.3:58419367-58419386 | MS.gene049712:CDS | 55.0% | |
| TGGTGTGAGGGTGGTTATGC+CGG | + | chr3.3:58419484-58419503 | MS.gene049712:CDS | 55.0% | |
| ! | ATTGGCTACCGTGGCGGAAA+AGG | + | chr3.3:58419514-58419533 | MS.gene049712:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 58419296 | 58419634 | 58419296 | ID=MS.gene049712 |
| chr3.3 | mRNA | 58419296 | 58419634 | 58419296 | ID=MS.gene049712.t1;Parent=MS.gene049712 |
| chr3.3 | exon | 58419296 | 58419634 | 58419296 | ID=MS.gene049712.t1.exon1;Parent=MS.gene049712.t1 |
| chr3.3 | CDS | 58419296 | 58419634 | 58419296 | ID=cds.MS.gene049712.t1;Parent=MS.gene049712.t1 |
| Gene Sequence |
| Protein sequence |
