Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58300.t1 | XP_013444920.1 | 94 | 183 | 11 | 0 | 1 | 183 | 1 | 183 | 1.20E-93 | 352.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58300.t1 | Q58FX0 | 32.8 | 134 | 74 | 6 | 6 | 130 | 8 | 134 | 5.7e-07 | 55.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58300.t1 | A0A072TZI9 | 94.0 | 183 | 11 | 0 | 1 | 183 | 1 | 183 | 8.8e-94 | 352.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049712 | MS.gene58300 | 0.803074 | 4.15E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene58300 | 0.802048 | 6.75E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58300.t1 | MTR_8g432490 | 93.989 | 183 | 11 | 0 | 1 | 183 | 1 | 183 | 4.50e-127 | 355 |
| MS.gene58300.t1 | MTR_8g432460 | 89.617 | 183 | 19 | 0 | 1 | 183 | 1 | 183 | 3.44e-122 | 342 |
| MS.gene58300.t1 | MTR_3g064780 | 86.667 | 180 | 24 | 0 | 1 | 180 | 1 | 180 | 1.50e-113 | 323 |
| MS.gene58300.t1 | MTR_8g432440 | 84.239 | 184 | 28 | 1 | 1 | 183 | 1 | 184 | 6.94e-113 | 319 |
| MS.gene58300.t1 | MTR_8g432420 | 84.239 | 184 | 28 | 1 | 1 | 183 | 1 | 184 | 6.94e-113 | 319 |
| MS.gene58300.t1 | MTR_8g432470 | 83.152 | 184 | 30 | 1 | 1 | 183 | 1 | 184 | 3.76e-110 | 312 |
| MS.gene58300.t1 | MTR_8g432450 | 84.181 | 177 | 26 | 2 | 1 | 175 | 1 | 177 | 1.73e-104 | 297 |
| MS.gene58300.t1 | MTR_8g432480 | 52.841 | 176 | 61 | 5 | 5 | 174 | 7 | 166 | 8.33e-49 | 157 |
| MS.gene58300.t1 | MTR_7g076000 | 36.872 | 179 | 81 | 8 | 5 | 174 | 2 | 157 | 8.59e-25 | 95.1 |
| MS.gene58300.t1 | MTR_8g432400 | 38.378 | 185 | 93 | 8 | 5 | 179 | 2 | 175 | 1.19e-22 | 89.7 |
| MS.gene58300.t1 | MTR_8g432370 | 36.413 | 184 | 97 | 7 | 5 | 179 | 2 | 174 | 5.75e-21 | 85.5 |
| MS.gene58300.t1 | MTR_8g432350 | 36.264 | 182 | 96 | 7 | 5 | 177 | 2 | 172 | 2.41e-20 | 83.6 |
| MS.gene58300.t1 | MTR_8g432390 | 37.297 | 185 | 95 | 8 | 5 | 179 | 2 | 175 | 3.18e-20 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATGGAGTTATTCATTT+TGG | 0.202103 | 8.2:-65514101 | MS.gene58300:CDS |
| AACTCTAATGGTGGTGTTAT+TGG | 0.227722 | 8.2:-65513966 | MS.gene58300:CDS |
| AACACCACCATTAGAGTTCT+TGG | 0.255915 | 8.2:+65513971 | None:intergenic |
| CCTGGGTAATTCCACCTAAA+AGG | 0.262534 | 8.2:+65513939 | None:intergenic |
| GCGATTCTTGCCACCCCAAT+AGG | 0.288851 | 8.2:+65514206 | None:intergenic |
| TTCAAAAGTGTTCGCCCTAT+TGG | 0.292098 | 8.2:-65514221 | MS.gene58300:CDS |
| TCAAAAGTGTTCGCCCTATT+GGG | 0.316840 | 8.2:-65514220 | MS.gene58300:CDS |
| GATTGGGAAGGGAGGCAAAA+TGG | 0.319629 | 8.2:-65514116 | MS.gene58300:CDS |
| TGAGTTATAAATTGAGGGAT+TGG | 0.355220 | 8.2:-65514133 | MS.gene58300:CDS |
| AGTTATGGTGATCGGTTAAC+AGG | 0.361407 | 8.2:-65514008 | MS.gene58300:CDS |
| ACAAATCTTTAAACAATGCC+TGG | 0.408723 | 8.2:+65513921 | None:intergenic |
| CCGTTTCGGATATTCTAAAC+GGG | 0.419922 | 8.2:-65514184 | MS.gene58300:CDS |
| TGAAAGATAATGACGATTGC+GGG | 0.421318 | 8.2:-65514337 | MS.gene58300:CDS |
| GCCGTTTCGGATATTCTAAA+CGG | 0.421563 | 8.2:-65514185 | MS.gene58300:CDS |
| ATCGGTTACGGTGGCAGAAA+AGG | 0.421967 | 8.2:-65514051 | MS.gene58300:CDS |
| TATTCTAAACGGGGATGAAT+CGG | 0.432147 | 8.2:-65514174 | MS.gene58300:CDS |
| TGAAAATATTCATGTAACTA+AGG | 0.454486 | 8.2:-65514414 | MS.gene58300:CDS |
| GGTGGCAAGAATCGCCGTTT+CGG | 0.455962 | 8.2:-65514198 | MS.gene58300:CDS |
| CGATTCTTGCCACCCCAATA+GGG | 0.466572 | 8.2:+65514207 | None:intergenic |
| GAGTTATAAATTGAGGGATT+GGG | 0.478191 | 8.2:-65514132 | MS.gene58300:CDS |
| CGGTTAACAGGAATTGATGT+TGG | 0.481111 | 8.2:-65513996 | MS.gene58300:CDS |
| TGGTGTGAGGGTGGTTCTGC+CGG | 0.495716 | 8.2:-65514081 | MS.gene58300:CDS |
| CTCACGTACATTAGAAATAA+CGG | 0.497810 | 8.2:-65514447 | MS.gene58300:CDS |
| ATAAATTGAGGGATTGGGAA+GGG | 0.506904 | 8.2:-65514127 | MS.gene58300:CDS |
| TTAGAAATAACGGTTATATC+CGG | 0.522816 | 8.2:-65514437 | MS.gene58300:CDS |
| TGCAAGGTCAATCACGTTTG+AGG | 0.523501 | 8.2:-65514258 | MS.gene58300:CDS |
| ATGGCGGATGGAAAGAGTTA+TGG | 0.523652 | 8.2:-65514023 | MS.gene58300:CDS |
| GATGCTTATGTTGTCGTTCG+AGG | 0.529974 | 8.2:-65514392 | MS.gene58300:CDS |
| GTTGGTACCAAGAACTCTAA+TGG | 0.534197 | 8.2:-65513978 | MS.gene58300:CDS |
| CCCGTTTAGAATATCCGAAA+CGG | 0.552046 | 8.2:+65514184 | None:intergenic |
| CAAATCTTTAAACAATGCCT+GGG | 0.558166 | 8.2:+65513922 | None:intergenic |
| TGAACATGCCATTGCATGCA+AGG | 0.566598 | 8.2:-65514274 | MS.gene58300:CDS |
| CAAAAGTGTTCGCCCTATTG+GGG | 0.566660 | 8.2:-65514219 | MS.gene58300:CDS |
| AATTGAGGGATTGGGAAGGG+AGG | 0.569326 | 8.2:-65514124 | MS.gene58300:CDS |
| GCCGGAGAAAACATCGGTTA+CGG | 0.571984 | 8.2:-65514063 | MS.gene58300:CDS |
| TATAAATTGAGGGATTGGGA+AGG | 0.575657 | 8.2:-65514128 | MS.gene58300:CDS |
| ATGGAAAGAGTTATGGTGAT+CGG | 0.595704 | 8.2:-65514016 | MS.gene58300:CDS |
| GTGAAAGATAATGACGATTG+CGG | 0.597093 | 8.2:-65514338 | MS.gene58300:CDS |
| GAAAAGGAAACAATGGCGGA+TGG | 0.601599 | 8.2:-65514035 | MS.gene58300:CDS |
| CGTTATTTCTAATGTACGTG+AGG | 0.605270 | 8.2:+65514448 | None:intergenic |
| GTGATTGACCTTGCATGCAA+TGG | 0.618595 | 8.2:+65514266 | None:intergenic |
| AAGTGTTCGCCCTATTGGGG+TGG | 0.646267 | 8.2:-65514216 | MS.gene58300:CDS |
| GGTGGCAGAAAAGGAAACAA+TGG | 0.664839 | 8.2:-65514042 | MS.gene58300:CDS |
| CGTTTCGGATATTCTAAACG+GGG | 0.669234 | 8.2:-65514183 | MS.gene58300:CDS |
| GGTTCTGCCGGAGAAAACAT+CGG | 0.670138 | 8.2:-65514069 | MS.gene58300:CDS |
| GGCAGAAAAGGAAACAATGG+CGG | 0.682285 | 8.2:-65514039 | MS.gene58300:CDS |
| GGAGAAAACATCGGTTACGG+TGG | 0.688629 | 8.2:-65514060 | MS.gene58300:CDS |
| GGTACCAAGAACTCTAATGG+TGG | 0.759836 | 8.2:-65513975 | MS.gene58300:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTGAGTTATAAATTGA+GGG | - | chr8.2:65514249-65514268 | MS.gene58300:CDS | 15.0% |
| !! | TAATTGTTACACAACAAAAA+TGG | - | chr8.2:65514027-65514046 | MS.gene58300:CDS | 20.0% |
| !! | TGAAAATATTCATGTAACTA+AGG | - | chr8.2:65513973-65513992 | MS.gene58300:CDS | 20.0% |
| !!! | GAATTTTGAGTTATAAATTG+AGG | - | chr8.2:65514248-65514267 | MS.gene58300:CDS | 20.0% |
| ! | TTAGAAATAACGGTTATATC+CGG | - | chr8.2:65513950-65513969 | MS.gene58300:CDS | 25.0% |
| !! | AGTTACATGAATATTTTCAC+CGG | + | chr8.2:65513972-65513991 | None:intergenic | 25.0% |
| !! | CAAAATGGAGTTATTCATTT+TGG | - | chr8.2:65514286-65514305 | MS.gene58300:CDS | 25.0% |
| CTCACGTACATTAGAAATAA+CGG | - | chr8.2:65513940-65513959 | MS.gene58300:CDS | 30.0% | |
| GAGTTATAAATTGAGGGATT+GGG | - | chr8.2:65514255-65514274 | MS.gene58300:CDS | 30.0% | |
| TGAGTTATAAATTGAGGGAT+TGG | - | chr8.2:65514254-65514273 | MS.gene58300:CDS | 30.0% | |
| ! | AAAACTCGAGTTTTCTTTCA+TGG | - | chr8.2:65514074-65514093 | MS.gene58300:CDS | 30.0% |
| ! | TTATTGGTATTCCTTTTAGG+TGG | - | chr8.2:65514437-65514456 | MS.gene58300:CDS | 30.0% |
| !! | AGTTATTCATTTTGGTGTGA+GGG | - | chr8.2:65514294-65514313 | MS.gene58300:CDS | 30.0% |
| !! | GTGTTATTGGTATTCCTTTT+AGG | - | chr8.2:65514434-65514453 | MS.gene58300:CDS | 30.0% |
| ATAAATTGAGGGATTGGGAA+GGG | - | chr8.2:65514260-65514279 | MS.gene58300:CDS | 35.0% | |
| ATGGAAAGAGTTATGGTGAT+CGG | - | chr8.2:65514371-65514390 | MS.gene58300:CDS | 35.0% | |
| GTGAAAGATAATGACGATTG+CGG | - | chr8.2:65514049-65514068 | MS.gene58300:CDS | 35.0% | |
| TATAAATTGAGGGATTGGGA+AGG | - | chr8.2:65514259-65514278 | MS.gene58300:CDS | 35.0% | |
| TATTCTAAACGGGGATGAAT+CGG | - | chr8.2:65514213-65514232 | MS.gene58300:CDS | 35.0% | |
| TGAAAGATAATGACGATTGC+GGG | - | chr8.2:65514050-65514069 | MS.gene58300:CDS | 35.0% | |
| !! | AACTCTAATGGTGGTGTTAT+TGG | - | chr8.2:65514421-65514440 | MS.gene58300:CDS | 35.0% |
| !! | CGTTATTTCTAATGTACGTG+AGG | + | chr8.2:65513942-65513961 | None:intergenic | 35.0% |
| !! | GAGTTATTCATTTTGGTGTG+AGG | - | chr8.2:65514293-65514312 | MS.gene58300:CDS | 35.0% |
| AACACCACCATTAGAGTTCT+TGG | + | chr8.2:65514419-65514438 | None:intergenic | 40.0% | |
| AGTTATGGTGATCGGTTAAC+AGG | - | chr8.2:65514379-65514398 | MS.gene58300:CDS | 40.0% | |
| CCGTTTCGGATATTCTAAAC+GGG | - | chr8.2:65514203-65514222 | MS.gene58300:CDS | 40.0% | |
| CGGTTAACAGGAATTGATGT+TGG | - | chr8.2:65514391-65514410 | MS.gene58300:CDS | 40.0% | |
| CGTTTCGGATATTCTAAACG+GGG | - | chr8.2:65514204-65514223 | MS.gene58300:CDS | 40.0% | |
| GCCGTTTCGGATATTCTAAA+CGG | - | chr8.2:65514202-65514221 | MS.gene58300:CDS | 40.0% | |
| GTTGGTACCAAGAACTCTAA+TGG | - | chr8.2:65514409-65514428 | MS.gene58300:CDS | 40.0% | |
| ! | CCCGTTTAGAATATCCGAAA+CGG | + | chr8.2:65514206-65514225 | None:intergenic | 40.0% |
| !! | TATTCATTTTGGTGTGAGGG+TGG | - | chr8.2:65514297-65514316 | MS.gene58300:CDS | 40.0% |
| !! | TCAAAAGTGTTCGCCCTATT+GGG | - | chr8.2:65514167-65514186 | MS.gene58300:CDS | 40.0% |
| !! | TTCAAAAGTGTTCGCCCTAT+TGG | - | chr8.2:65514166-65514185 | MS.gene58300:CDS | 40.0% |
| CCTGGGTAATTCCACCTAAA+AGG | + | chr8.2:65514451-65514470 | None:intergenic | 45.0% | |
| GATGCTTATGTTGTCGTTCG+AGG | - | chr8.2:65513995-65514014 | MS.gene58300:CDS | 45.0% | |
| GGCAGAAAAGGAAACAATGG+CGG | - | chr8.2:65514348-65514367 | MS.gene58300:CDS | 45.0% | |
| GGTACCAAGAACTCTAATGG+TGG | - | chr8.2:65514412-65514431 | MS.gene58300:CDS | 45.0% | |
| GGTGGCAGAAAAGGAAACAA+TGG | - | chr8.2:65514345-65514364 | MS.gene58300:CDS | 45.0% | |
| GTGATTGACCTTGCATGCAA+TGG | + | chr8.2:65514124-65514143 | None:intergenic | 45.0% | |
| TGAACATGCCATTGCATGCA+AGG | - | chr8.2:65514113-65514132 | MS.gene58300:CDS | 45.0% | |
| TGCAAGGTCAATCACGTTTG+AGG | - | chr8.2:65514129-65514148 | MS.gene58300:CDS | 45.0% | |
| ! | ACCGTAACCGATGTTTTCTC+CGG | + | chr8.2:65514328-65514347 | None:intergenic | 45.0% |
| ! | GAAAAGGAAACAATGGCGGA+TGG | - | chr8.2:65514352-65514371 | MS.gene58300:CDS | 45.0% |
| !! | ATGGCGGATGGAAAGAGTTA+TGG | - | chr8.2:65514364-65514383 | MS.gene58300:CDS | 45.0% |
| !! | CAAAAGTGTTCGCCCTATTG+GGG | - | chr8.2:65514168-65514187 | MS.gene58300:CDS | 45.0% |
| !! | CCTTTTAGGTGGAATTACCC+AGG | - | chr8.2:65514448-65514467 | MS.gene58300:CDS | 45.0% |
| AATTGAGGGATTGGGAAGGG+AGG | - | chr8.2:65514263-65514282 | MS.gene58300:CDS | 50.0% | |
| ATCGGTTACGGTGGCAGAAA+AGG | - | chr8.2:65514336-65514355 | MS.gene58300:CDS | 50.0% | |
| CGATTCTTGCCACCCCAATA+GGG | + | chr8.2:65514183-65514202 | None:intergenic | 50.0% | |
| GATTGGGAAGGGAGGCAAAA+TGG | - | chr8.2:65514271-65514290 | MS.gene58300:CDS | 50.0% | |
| GGTTCTGCCGGAGAAAACAT+CGG | - | chr8.2:65514318-65514337 | MS.gene58300:CDS | 50.0% | |
| ! | GCCGGAGAAAACATCGGTTA+CGG | - | chr8.2:65514324-65514343 | MS.gene58300:CDS | 50.0% |
| ! | GGAGAAAACATCGGTTACGG+TGG | - | chr8.2:65514327-65514346 | MS.gene58300:CDS | 50.0% |
| AAGTGTTCGCCCTATTGGGG+TGG | - | chr8.2:65514171-65514190 | MS.gene58300:CDS | 55.0% | |
| GCGATTCTTGCCACCCCAAT+AGG | + | chr8.2:65514184-65514203 | None:intergenic | 55.0% | |
| GGTGGCAAGAATCGCCGTTT+CGG | - | chr8.2:65514189-65514208 | MS.gene58300:CDS | 55.0% | |
| ! | TGGTGTGAGGGTGGTTCTGC+CGG | - | chr8.2:65514306-65514325 | MS.gene58300:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 65513929 | 65514480 | 65513929 | ID=MS.gene58300 |
| chr8.2 | mRNA | 65513929 | 65514480 | 65513929 | ID=MS.gene58300.t1;Parent=MS.gene58300 |
| chr8.2 | exon | 65513929 | 65514480 | 65513929 | ID=MS.gene58300.t1.exon1;Parent=MS.gene58300.t1 |
| chr8.2 | CDS | 65513929 | 65514480 | 65513929 | ID=cds.MS.gene58300.t1;Parent=MS.gene58300.t1 |
| Gene Sequence |
| Protein sequence |
