Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36870.t1 | XP_013444920.1 | 94 | 183 | 11 | 0 | 1 | 183 | 1 | 183 | 1.20E-93 | 352.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36870.t1 | A0A072TZI9 | 94.0 | 183 | 11 | 0 | 1 | 183 | 1 | 183 | 8.8e-94 | 352.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene36870 | 0.844124 | 9.18E-59 | -1.69E-46 |
| MS.gene049712 | MS.gene36870 | 0.802334 | 5.89E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene36870 | 0.812081 | 5.07E-51 | -1.69E-46 |
| MS.gene04998 | MS.gene36870 | 0.837517 | 4.96E-57 | -1.69E-46 |
| MS.gene050414 | MS.gene36870 | 0.808267 | 3.37E-50 | -1.69E-46 |
| MS.gene050654 | MS.gene36870 | 0.805396 | 1.36E-49 | -1.69E-46 |
| MS.gene052016 | MS.gene36870 | 0.815557 | 8.70E-52 | -1.69E-46 |
| MS.gene052493 | MS.gene36870 | 0.808435 | 3.10E-50 | -1.69E-46 |
| MS.gene052533 | MS.gene36870 | 0.803071 | 4.15E-49 | -1.69E-46 |
| MS.gene053094 | MS.gene36870 | 0.80158 | 8.42E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene36870 | 0.826304 | 2.92E-54 | -1.69E-46 |
| MS.gene053726 | MS.gene36870 | 0.808909 | 2.46E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene36870 | 0.823921 | 1.07E-53 | -1.69E-46 |
| MS.gene05439 | MS.gene36870 | 0.819345 | 1.22E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene36870 | 0.879068 | 1.69E-69 | -1.69E-46 |
| MS.gene054893 | MS.gene36870 | 0.883328 | 4.92E-71 | -1.69E-46 |
| MS.gene055147 | MS.gene36870 | 0.805309 | 1.42E-49 | -1.69E-46 |
| MS.gene055226 | MS.gene36870 | 0.808596 | 2.87E-50 | -1.69E-46 |
| MS.gene056699 | MS.gene36870 | 0.801753 | 7.76E-49 | -1.69E-46 |
| MS.gene057155 | MS.gene36870 | 0.817924 | 2.56E-52 | -1.69E-46 |
| MS.gene057622 | MS.gene36870 | 0.833033 | 6.73E-56 | -1.69E-46 |
| MS.gene058159 | MS.gene36870 | 0.800313 | 1.53E-48 | -1.69E-46 |
| MS.gene058429 | MS.gene36870 | 0.808362 | 3.22E-50 | -1.69E-46 |
| MS.gene058592 | MS.gene36870 | 0.826564 | 2.53E-54 | -1.69E-46 |
| MS.gene05952 | MS.gene36870 | 0.817027 | 4.08E-52 | -1.69E-46 |
| MS.gene05985 | MS.gene36870 | -0.820584 | 6.35E-53 | -1.69E-46 |
| MS.gene06023 | MS.gene36870 | 0.805409 | 1.35E-49 | -1.69E-46 |
| MS.gene060259 | MS.gene36870 | 0.841956 | 3.47E-58 | -1.69E-46 |
| MS.gene060412 | MS.gene36870 | 0.86532 | 6.43E-65 | -1.69E-46 |
| MS.gene060413 | MS.gene36870 | 0.841963 | 3.45E-58 | -1.69E-46 |
| MS.gene060840 | MS.gene36870 | 0.806393 | 8.41E-50 | -1.69E-46 |
| MS.gene060932 | MS.gene36870 | 0.812545 | 4.02E-51 | -1.69E-46 |
| MS.gene061047 | MS.gene36870 | 0.841491 | 4.60E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36870.t1 | MTR_8g432490 | 93.989 | 183 | 11 | 0 | 1 | 183 | 1 | 183 | 2.09e-127 | 355 |
| MS.gene36870.t1 | MTR_8g432460 | 89.071 | 183 | 20 | 0 | 1 | 183 | 1 | 183 | 1.13e-120 | 338 |
| MS.gene36870.t1 | MTR_3g064780 | 86.667 | 180 | 24 | 0 | 1 | 180 | 1 | 180 | 1.10e-113 | 323 |
| MS.gene36870.t1 | MTR_8g432440 | 84.239 | 184 | 28 | 1 | 1 | 183 | 1 | 184 | 8.10e-113 | 319 |
| MS.gene36870.t1 | MTR_8g432420 | 84.239 | 184 | 28 | 1 | 1 | 183 | 1 | 184 | 8.10e-113 | 319 |
| MS.gene36870.t1 | MTR_8g432470 | 83.696 | 184 | 29 | 1 | 1 | 183 | 1 | 184 | 1.14e-110 | 313 |
| MS.gene36870.t1 | MTR_8g432450 | 84.181 | 177 | 26 | 2 | 1 | 175 | 1 | 177 | 2.48e-104 | 297 |
| MS.gene36870.t1 | MTR_8g432480 | 50.568 | 176 | 65 | 5 | 5 | 174 | 7 | 166 | 6.72e-45 | 147 |
| MS.gene36870.t1 | MTR_7g076000 | 36.313 | 179 | 82 | 8 | 5 | 174 | 2 | 157 | 5.24e-23 | 90.5 |
| MS.gene36870.t1 | MTR_8g432400 | 37.705 | 183 | 97 | 6 | 5 | 179 | 2 | 175 | 1.39e-22 | 89.7 |
| MS.gene36870.t1 | MTR_8g432370 | 35.714 | 182 | 101 | 5 | 5 | 179 | 2 | 174 | 2.53e-21 | 86.3 |
| MS.gene36870.t1 | MTR_8g432350 | 35.556 | 180 | 100 | 5 | 5 | 177 | 2 | 172 | 1.28e-20 | 84.3 |
| MS.gene36870.t1 | MTR_8g432390 | 36.612 | 183 | 99 | 6 | 5 | 179 | 2 | 175 | 1.70e-20 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAATGGAGTTAATCATTT+TGG | 0.193044 | 8.4:-64121336 | MS.gene36870:CDS |
| AACTCTAATGGTGGTGTTAT+TGG | 0.227722 | 8.4:-64121201 | MS.gene36870:CDS |
| AACACCACCATTAGAGTTCT+TGG | 0.255915 | 8.4:+64121206 | None:intergenic |
| CCTGGGTAATTCCACCTAAA+AGG | 0.262534 | 8.4:+64121174 | None:intergenic |
| AAAACTCGAGTTATCTTTCA+TGG | 0.312100 | 8.4:-64121548 | MS.gene36870:CDS |
| GATTGGGAAGGGAGGCAAAA+TGG | 0.319629 | 8.4:-64121351 | MS.gene36870:CDS |
| AGTTATGGTGATCGGTTAAC+TGG | 0.339421 | 8.4:-64121243 | MS.gene36870:CDS |
| TTCAAAAGTGTTCGTCCTAT+TGG | 0.350867 | 8.4:-64121456 | MS.gene36870:CDS |
| TGAGTTATAAATTGAGGGAT+TGG | 0.355220 | 8.4:-64121368 | MS.gene36870:CDS |
| ATCGGTTACGGTGGCGGAAA+AGG | 0.359368 | 8.4:-64121286 | MS.gene36870:CDS |
| GCGATTCTTGCCACGCCAAT+AGG | 0.376994 | 8.4:+64121441 | None:intergenic |
| ACAAATCTTTAAACAATGCC+TGG | 0.408723 | 8.4:+64121156 | None:intergenic |
| GCCGTTTCAGATATTCTAAA+CGG | 0.413963 | 8.4:-64121420 | MS.gene36870:CDS |
| TATTCTAAACGGGGCTGAAT+CGG | 0.426061 | 8.4:-64121409 | MS.gene36870:CDS |
| CCGTTTCAGATATTCTAAAC+GGG | 0.426321 | 8.4:-64121419 | MS.gene36870:CDS |
| TGAAAGATAATGATGATTGC+GGG | 0.447649 | 8.4:-64121572 | MS.gene36870:CDS |
| GAGTTATAAATTGAGGGATT+GGG | 0.478191 | 8.4:-64121367 | MS.gene36870:CDS |
| GAAAACATCGGTTACGGTGG+CGG | 0.489652 | 8.4:-64121292 | MS.gene36870:CDS |
| ATGCATTTGTTGTCGTTCGA+GGG | 0.491000 | 8.4:-64121626 | MS.gene36870:CDS |
| CTCACGTACATTAGAAATAA+CGG | 0.497810 | 8.4:-64121682 | MS.gene36870:CDS |
| GATGCATTTGTTGTCGTTCG+AGG | 0.505000 | 8.4:-64121627 | MS.gene36870:CDS |
| ATAAATTGAGGGATTGGGAA+GGG | 0.506904 | 8.4:-64121362 | MS.gene36870:CDS |
| TTAGAAATAACGGTTATATC+CGG | 0.522816 | 8.4:-64121672 | MS.gene36870:CDS |
| TGCAAGGTCAATCACGTTTG+AGG | 0.523501 | 8.4:-64121493 | MS.gene36870:CDS |
| ATGGCGGATGGAAAGAGTTA+TGG | 0.523652 | 8.4:-64121258 | MS.gene36870:CDS |
| GTTGGTACCAAGAACTCTAA+TGG | 0.534197 | 8.4:-64121213 | MS.gene36870:CDS |
| CCCGTTTAGAATATCTGAAA+CGG | 0.537134 | 8.4:+64121419 | None:intergenic |
| CGGTTAACTGGAATTGATGT+TGG | 0.538648 | 8.4:-64121231 | MS.gene36870:CDS |
| CAAATCTTTAAACAATGCCT+GGG | 0.558166 | 8.4:+64121157 | None:intergenic |
| TGCATTTGTTGTCGTTCGAG+GGG | 0.563628 | 8.4:-64121625 | MS.gene36870:CDS |
| GCCTGAGAAAACATCGGTTA+CGG | 0.563675 | 8.4:-64121298 | MS.gene36870:CDS |
| TGAACATGCCATTGCATGCA+AGG | 0.566598 | 8.4:-64121509 | MS.gene36870:CDS |
| GTGAAAGATAATGATGATTG+CGG | 0.569123 | 8.4:-64121573 | MS.gene36870:CDS |
| AATTGAGGGATTGGGAAGGG+AGG | 0.569326 | 8.4:-64121359 | MS.gene36870:CDS |
| TATAAATTGAGGGATTGGGA+AGG | 0.575657 | 8.4:-64121363 | MS.gene36870:CDS |
| ATGGAAAGAGTTATGGTGAT+CGG | 0.595704 | 8.4:-64121251 | MS.gene36870:CDS |
| GAAAAGGAAACAATGGCGGA+TGG | 0.603995 | 8.4:-64121270 | MS.gene36870:CDS |
| CGTTATTTCTAATGTACGTG+AGG | 0.605270 | 8.4:+64121683 | None:intergenic |
| GTGATTGACCTTGCATGCAA+TGG | 0.618595 | 8.4:+64121501 | None:intergenic |
| TGAAAATATTCATGTAACTG+AGG | 0.625588 | 8.4:-64121649 | MS.gene36870:CDS |
| CGTTTCAGATATTCTAAACG+GGG | 0.637516 | 8.4:-64121418 | MS.gene36870:CDS |
| AAGTGTTCGTCCTATTGGCG+TGG | 0.646267 | 8.4:-64121451 | MS.gene36870:CDS |
| GGTGGCGGAAAAGGAAACAA+TGG | 0.668224 | 8.4:-64121277 | MS.gene36870:CDS |
| TGAGAAAACATCGGTTACGG+TGG | 0.678174 | 8.4:-64121295 | MS.gene36870:CDS |
| GGTTGTGCCTGAGAAAACAT+CGG | 0.692346 | 8.4:-64121304 | MS.gene36870:CDS |
| GGCGGAAAAGGAAACAATGG+CGG | 0.701876 | 8.4:-64121274 | MS.gene36870:CDS |
| GGTACCAAGAACTCTAATGG+TGG | 0.759836 | 8.4:-64121210 | MS.gene36870:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTGAGTTATAAATTGA+GGG | - | chr8.4:64121484-64121503 | MS.gene36870:CDS | 15.0% |
| !!! | GAATTTTGAGTTATAAATTG+AGG | - | chr8.4:64121483-64121502 | MS.gene36870:CDS | 20.0% |
| ! | TGAAAATATTCATGTAACTG+AGG | - | chr8.4:64121208-64121227 | MS.gene36870:CDS | 25.0% |
| ! | TTAGAAATAACGGTTATATC+CGG | - | chr8.4:64121185-64121204 | MS.gene36870:CDS | 25.0% |
| !! | AGTTACATGAATATTTTCAC+CGG | + | chr8.4:64121207-64121226 | None:intergenic | 25.0% |
| !! | CAAAATGGAGTTAATCATTT+TGG | - | chr8.4:64121521-64121540 | MS.gene36870:CDS | 25.0% |
| AAAACTCGAGTTATCTTTCA+TGG | - | chr8.4:64121309-64121328 | MS.gene36870:CDS | 30.0% | |
| CTCACGTACATTAGAAATAA+CGG | - | chr8.4:64121175-64121194 | MS.gene36870:CDS | 30.0% | |
| GAGTTATAAATTGAGGGATT+GGG | - | chr8.4:64121490-64121509 | MS.gene36870:CDS | 30.0% | |
| GTGAAAGATAATGATGATTG+CGG | - | chr8.4:64121284-64121303 | MS.gene36870:CDS | 30.0% | |
| TGAAAGATAATGATGATTGC+GGG | - | chr8.4:64121285-64121304 | MS.gene36870:CDS | 30.0% | |
| TGAGTTATAAATTGAGGGAT+TGG | - | chr8.4:64121489-64121508 | MS.gene36870:CDS | 30.0% | |
| ! | TTATTGGTATTCCTTTTAGG+TGG | - | chr8.4:64121672-64121691 | MS.gene36870:CDS | 30.0% |
| !! | AGTTAATCATTTTGGTGTGA+GGG | - | chr8.4:64121529-64121548 | MS.gene36870:CDS | 30.0% |
| !! | GTGTTATTGGTATTCCTTTT+AGG | - | chr8.4:64121669-64121688 | MS.gene36870:CDS | 30.0% |
| ATAAATTGAGGGATTGGGAA+GGG | - | chr8.4:64121495-64121514 | MS.gene36870:CDS | 35.0% | |
| ATGGAAAGAGTTATGGTGAT+CGG | - | chr8.4:64121606-64121625 | MS.gene36870:CDS | 35.0% | |
| CCGTTTCAGATATTCTAAAC+GGG | - | chr8.4:64121438-64121457 | MS.gene36870:CDS | 35.0% | |
| CGTTTCAGATATTCTAAACG+GGG | - | chr8.4:64121439-64121458 | MS.gene36870:CDS | 35.0% | |
| GCCGTTTCAGATATTCTAAA+CGG | - | chr8.4:64121437-64121456 | MS.gene36870:CDS | 35.0% | |
| TATAAATTGAGGGATTGGGA+AGG | - | chr8.4:64121494-64121513 | MS.gene36870:CDS | 35.0% | |
| ! | CCCGTTTAGAATATCTGAAA+CGG | + | chr8.4:64121441-64121460 | None:intergenic | 35.0% |
| !! | AACTCTAATGGTGGTGTTAT+TGG | - | chr8.4:64121656-64121675 | MS.gene36870:CDS | 35.0% |
| !! | CGTTATTTCTAATGTACGTG+AGG | + | chr8.4:64121177-64121196 | None:intergenic | 35.0% |
| !! | GAGTTAATCATTTTGGTGTG+AGG | - | chr8.4:64121528-64121547 | MS.gene36870:CDS | 35.0% |
| !! | TTCAAAAGTGTTCGTCCTAT+TGG | - | chr8.4:64121401-64121420 | MS.gene36870:CDS | 35.0% |
| AACACCACCATTAGAGTTCT+TGG | + | chr8.4:64121654-64121673 | None:intergenic | 40.0% | |
| AGTTATGGTGATCGGTTAAC+TGG | - | chr8.4:64121614-64121633 | MS.gene36870:CDS | 40.0% | |
| CGGTTAACTGGAATTGATGT+TGG | - | chr8.4:64121626-64121645 | MS.gene36870:CDS | 40.0% | |
| GTTGGTACCAAGAACTCTAA+TGG | - | chr8.4:64121644-64121663 | MS.gene36870:CDS | 40.0% | |
| TATTCTAAACGGGGCTGAAT+CGG | - | chr8.4:64121448-64121467 | MS.gene36870:CDS | 40.0% | |
| !! | ATGCATTTGTTGTCGTTCGA+GGG | - | chr8.4:64121231-64121250 | MS.gene36870:CDS | 40.0% |
| !! | TAATCATTTTGGTGTGAGGG+TGG | - | chr8.4:64121532-64121551 | MS.gene36870:CDS | 40.0% |
| CCTGGGTAATTCCACCTAAA+AGG | + | chr8.4:64121686-64121705 | None:intergenic | 45.0% | |
| GGTACCAAGAACTCTAATGG+TGG | - | chr8.4:64121647-64121666 | MS.gene36870:CDS | 45.0% | |
| GGTTGTGCCTGAGAAAACAT+CGG | - | chr8.4:64121553-64121572 | MS.gene36870:CDS | 45.0% | |
| GTGATTGACCTTGCATGCAA+TGG | + | chr8.4:64121359-64121378 | None:intergenic | 45.0% | |
| TGAACATGCCATTGCATGCA+AGG | - | chr8.4:64121348-64121367 | MS.gene36870:CDS | 45.0% | |
| TGCAAGGTCAATCACGTTTG+AGG | - | chr8.4:64121364-64121383 | MS.gene36870:CDS | 45.0% | |
| ! | ACCGTAACCGATGTTTTCTC+AGG | + | chr8.4:64121563-64121582 | None:intergenic | 45.0% |
| ! | GAAAAGGAAACAATGGCGGA+TGG | - | chr8.4:64121587-64121606 | MS.gene36870:CDS | 45.0% |
| ! | GCCTGAGAAAACATCGGTTA+CGG | - | chr8.4:64121559-64121578 | MS.gene36870:CDS | 45.0% |
| ! | TGAGAAAACATCGGTTACGG+TGG | - | chr8.4:64121562-64121581 | MS.gene36870:CDS | 45.0% |
| !! | ATGGCGGATGGAAAGAGTTA+TGG | - | chr8.4:64121599-64121618 | MS.gene36870:CDS | 45.0% |
| !! | CCTTTTAGGTGGAATTACCC+AGG | - | chr8.4:64121683-64121702 | MS.gene36870:CDS | 45.0% |
| !! | GATGCATTTGTTGTCGTTCG+AGG | - | chr8.4:64121230-64121249 | MS.gene36870:CDS | 45.0% |
| !! | TGCATTTGTTGTCGTTCGAG+GGG | - | chr8.4:64121232-64121251 | MS.gene36870:CDS | 45.0% |
| AAGTGTTCGTCCTATTGGCG+TGG | - | chr8.4:64121406-64121425 | MS.gene36870:CDS | 50.0% | |
| AATTGAGGGATTGGGAAGGG+AGG | - | chr8.4:64121498-64121517 | MS.gene36870:CDS | 50.0% | |
| GATTGGGAAGGGAGGCAAAA+TGG | - | chr8.4:64121506-64121525 | MS.gene36870:CDS | 50.0% | |
| GGCGGAAAAGGAAACAATGG+CGG | - | chr8.4:64121583-64121602 | MS.gene36870:CDS | 50.0% | |
| ! | GAAAACATCGGTTACGGTGG+CGG | - | chr8.4:64121565-64121584 | MS.gene36870:CDS | 50.0% |
| !! | GGTGGCGGAAAAGGAAACAA+TGG | - | chr8.4:64121580-64121599 | MS.gene36870:CDS | 50.0% |
| GCGATTCTTGCCACGCCAAT+AGG | + | chr8.4:64121419-64121438 | None:intergenic | 55.0% | |
| !! | ATCGGTTACGGTGGCGGAAA+AGG | - | chr8.4:64121571-64121590 | MS.gene36870:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 64121164 | 64121715 | 64121164 | ID=MS.gene36870 |
| chr8.4 | mRNA | 64121164 | 64121715 | 64121164 | ID=MS.gene36870.t1;Parent=MS.gene36870 |
| chr8.4 | exon | 64121164 | 64121715 | 64121164 | ID=MS.gene36870.t1.exon1;Parent=MS.gene36870.t1 |
| chr8.4 | CDS | 64121164 | 64121715 | 64121164 | ID=cds.MS.gene36870.t1;Parent=MS.gene36870.t1 |
| Gene Sequence |
| Protein sequence |
