Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049752.t1 | XP_003606312.1 | 100 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 2.50E-44 | 187.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049752.t1 | O23249 | 94.3 | 88 | 4 | 1 | 1 | 88 | 1 | 87 | 2.4e-43 | 175.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049752.t1 | G7JM76 | 100.0 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 1.8e-44 | 187.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene86296 | MS.gene049752 | PPI |
| MS.gene54070 | MS.gene049752 | PPI |
| MS.gene29062 | MS.gene049752 | PPI |
| MS.gene021573 | MS.gene049752 | PPI |
| MS.gene73923 | MS.gene049752 | PPI |
| MS.gene031852 | MS.gene049752 | PPI |
| MS.gene30776 | MS.gene049752 | PPI |
| MS.gene015893 | MS.gene049752 | PPI |
| MS.gene053749 | MS.gene049752 | PPI |
| MS.gene94095 | MS.gene049752 | PPI |
| MS.gene57583 | MS.gene049752 | PPI |
| MS.gene049752 | MS.gene036003 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049752.t1 | MTR_4g057040 | 100.000 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 1.71e-62 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049752.t1 | AT2G27970 | 90.000 | 80 | 8 | 0 | 1 | 80 | 1 | 80 | 1.45e-51 | 156 |
| MS.gene049752.t1 | AT2G27960 | 97.183 | 71 | 2 | 0 | 1 | 71 | 1 | 71 | 5.79e-49 | 150 |
Find 26 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGGACAACCAGGCCCAGC+AGG | 0.260365 | 4.2:-46709718 | MS.gene049752:CDS |
| CCAGCATGCCCTGCTGGGCC+TGG | 0.333404 | 4.2:+46709709 | None:intergenic |
| AGCCACACATTATGCTCTTC+AGG | 0.335634 | 4.2:-46709764 | MS.gene049752:CDS |
| TTCAGAGAGAAGTCGATTCT+TGG | 0.337969 | 4.2:+46711552 | None:intergenic |
| ATTTGACCAGCATGCCCTGC+TGG | 0.343644 | 4.2:+46709703 | None:intergenic |
| GAGTGCAGCAGAGCCGTGGC+TGG | 0.380036 | 4.2:-46709812 | MS.gene049752:CDS |
| CAGAATGAATGGCGCGCAAT+TGG | 0.392088 | 4.2:-46709834 | MS.gene049752:CDS |
| GGAAGCAACTTCGCAACCTC+TGG | 0.415067 | 4.2:+46711574 | None:intergenic |
| GTTTAATCTTACAGAATGAA+TGG | 0.420697 | 4.2:-46709845 | MS.gene049752:intron |
| TTTGACCAGCATGCCCTGCT+GGG | 0.452510 | 4.2:+46709704 | None:intergenic |
| TCAGAGAGAAGTCGATTCTT+GGG | 0.456322 | 4.2:+46711553 | None:intergenic |
| CGAAATACTTCTCAGAGTAC+TGG | 0.464971 | 4.2:+46711732 | None:intergenic |
| CCAGGCCCAGCAGGGCATGC+TGG | 0.487057 | 4.2:-46709709 | MS.gene049752:CDS |
| TGCTGCTGCTGATAATTCAA+TGG | 0.507626 | 4.2:+46709738 | None:intergenic |
| CAGGACAACCAGGCCCAGCA+GGG | 0.518346 | 4.2:-46709717 | MS.gene049752:CDS |
| AGTGCAGCAGAGCCGTGGCT+GGG | 0.520271 | 4.2:-46709811 | MS.gene049752:CDS |
| AAGAGCATAATGTGTGGCTC+AGG | 0.531062 | 4.2:+46709768 | None:intergenic |
| TCGATGACACTTATGAGTAC+AGG | 0.536322 | 4.2:-46711712 | MS.gene049752:intron |
| GCATGTTGTTCTTCCTCCAG+AGG | 0.545874 | 4.2:-46711590 | MS.gene049752:CDS |
| AGCAACTTCGCAACCTCTGG+AGG | 0.549766 | 4.2:+46711577 | None:intergenic |
| ATTGAATTATCAGCAGCAGC+AGG | 0.557262 | 4.2:-46709736 | MS.gene049752:CDS |
| ATTGGAGTGCAGCAGAGCCG+TGG | 0.573620 | 4.2:-46709816 | MS.gene049752:CDS |
| TCAGCAGCAGCAGGACAACC+AGG | 0.580092 | 4.2:-46709727 | MS.gene049752:CDS |
| TTGCATAATGAACCCAGCCA+CGG | 0.629727 | 4.2:+46709799 | None:intergenic |
| CACACATTATGCTCTTCAGG+AGG | 0.695794 | 4.2:-46709761 | MS.gene049752:CDS |
| CTCCTGAAGAGCATAATGTG+TGG | 0.729925 | 4.2:+46709762 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGTTTTATTATTTTTTTG+TGG | - | chr4.2:46710099-46710118 | MS.gene049752:intron | 10.0% |
| !!! | GACATTTTTTTTTTTTTTTT+TGG | - | chr4.2:46709919-46709938 | MS.gene049752:intron | 10.0% |
| !!! | TTTTTGAAGATATTATATTT+TGG | - | chr4.2:46710070-46710089 | MS.gene049752:intron | 10.0% |
| !!! | AATTATCGATTAAAAGTTTA+AGG | + | chr4.2:46710814-46710833 | None:intergenic | 15.0% |
| !!! | ATTGTTTTTGTAAATAAGTA+CGG | - | chr4.2:46711443-46711462 | MS.gene049752:intron | 15.0% |
| !!! | ATTTGTAAAATTTAAGTTCA+AGG | + | chr4.2:46711268-46711287 | None:intergenic | 15.0% |
| !! | AATTCAGTATATTTGTGTAT+TGG | + | chr4.2:46710693-46710712 | None:intergenic | 20.0% |
| !! | AGTTATCAGTTAAAAGTTTA+AGG | + | chr4.2:46710759-46710778 | None:intergenic | 20.0% |
| !! | ATTAAATACACGATGAATTT+AGG | + | chr4.2:46711206-46711225 | None:intergenic | 20.0% |
| !! | CAATATCAACAAAAAAAACT+AGG | + | chr4.2:46709809-46709828 | None:intergenic | 20.0% |
| !! | GTTTGTTTAAATATTCTGTT+TGG | - | chr4.2:46711577-46711596 | MS.gene049752:CDS | 20.0% |
| !!! | AATACTGTATGATTTAAGTT+AGG | + | chr4.2:46710735-46710754 | None:intergenic | 20.0% |
| !!! | ATTTGATTTTGGCATTTTTT+AGG | - | chr4.2:46710196-46710215 | MS.gene049752:intron | 20.0% |
| !!! | TATGTCTTAGAGTTTATTTT+AGG | - | chr4.2:46710903-46710922 | MS.gene049752:intron | 20.0% |
| !!! | TGAAGACTTAAATTTGATTT+TGG | - | chr4.2:46710185-46710204 | MS.gene049752:intron | 20.0% |
| !!! | TTTGTTTTATGCTGAATTTT+TGG | - | chr4.2:46710018-46710037 | MS.gene049752:intron | 20.0% |
| !!! | TTTTTTTGTTGCTGTTTTTA+CGG | - | chr4.2:46710347-46710366 | MS.gene049752:intron | 20.0% |
| ! | CAAAAATAGATAATACCCTA+AGG | + | chr4.2:46710468-46710487 | None:intergenic | 25.0% |
| ! | CAGTTATCAACTAAAGTTAA+AGG | + | chr4.2:46710787-46710806 | None:intergenic | 25.0% |
| ! | GATCTAAGTGATTCTTAATT+TGG | - | chr4.2:46710874-46710893 | MS.gene049752:intron | 25.0% |
| ! | GTTTAATCTTACAGAATGAA+TGG | - | chr4.2:46711599-46711618 | MS.gene049752:intron | 25.0% |
| ! | TCTCAGTGTATTTAGATATT+GGG | + | chr4.2:46710947-46710966 | None:intergenic | 25.0% |
| ! | TTCAGATTGTTAAACAATGA+AGG | - | chr4.2:46711359-46711378 | MS.gene049752:intron | 25.0% |
| ! | TTCTCAGTGTATTTAGATAT+TGG | + | chr4.2:46710948-46710967 | None:intergenic | 25.0% |
| !! | CAAACAGTCTTTTCATTAAA+TGG | + | chr4.2:46711562-46711581 | None:intergenic | 25.0% |
| !! | TTATGATGATGACTTAATCT+TGG | - | chr4.2:46710436-46710455 | MS.gene049752:intron | 25.0% |
| !!! | AATGTTAATTTGTGCATGTT+AGG | - | chr4.2:46710135-46710154 | MS.gene049752:intron | 25.0% |
| !!! | AGTGATTCTTAATTTGGTTT+TGG | - | chr4.2:46710880-46710899 | MS.gene049752:intron | 25.0% |
| !!! | ATGATTTTTGGCTTTTGTTA+TGG | - | chr4.2:46711511-46711530 | MS.gene049752:intron | 25.0% |
| !!! | ATGTTAATTTGTGCATGTTA+GGG | - | chr4.2:46710136-46710155 | MS.gene049752:intron | 25.0% |
| !!! | ATTGTTTCTAACAGAAAGTT+TGG | - | chr4.2:46710653-46710672 | MS.gene049752:intron | 25.0% |
| !!! | TGATTTTTGGCTTTTGTTAT+GGG | - | chr4.2:46711512-46711531 | MS.gene049752:intron | 25.0% |
| !!! | TTGAGACGTATATTTGATTT+TGG | - | chr4.2:46710589-46710608 | MS.gene049752:intron | 25.0% |
| !!! | TTTGCATTCACTTTTGAAAT+GGG | - | chr4.2:46711053-46711072 | MS.gene049752:intron | 25.0% |
| AGTTCATTACACAGTGAATT+TGG | + | chr4.2:46710293-46710312 | None:intergenic | 30.0% | |
| GATAACTGAATATTACCATG+TGG | - | chr4.2:46710517-46710536 | MS.gene049752:intron | 30.0% | |
| GGCATAAAAATCATGTTTAG+AGG | + | chr4.2:46711185-46711204 | None:intergenic | 30.0% | |
| GGTATAAAAATCACGTTTAG+AGG | + | chr4.2:46710271-46710290 | None:intergenic | 30.0% | |
| GTTCATTACACAGTGAATTT+GGG | + | chr4.2:46710292-46710311 | None:intergenic | 30.0% | |
| TAATCTTGGTTAAGTCCTTA+GGG | - | chr4.2:46710450-46710469 | MS.gene049752:intron | 30.0% | |
| TTAATCTTGGTTAAGTCCTT+AGG | - | chr4.2:46710449-46710468 | MS.gene049752:intron | 30.0% | |
| ! | AGCTGAAGCAATATGATTTT+TGG | - | chr4.2:46711499-46711518 | MS.gene049752:intron | 30.0% |
| ! | AGTTATCAATCAAAAGTGTG+CGG | + | chr4.2:46711012-46711031 | None:intergenic | 30.0% |
| ! | GTTACTCTGATGATGAAATT+GGG | - | chr4.2:46710040-46710059 | MS.gene049752:intron | 30.0% |
| ! | TTACTCTGATGATGAAATTG+GGG | - | chr4.2:46710041-46710060 | MS.gene049752:intron | 30.0% |
| !! | GTTTGCATTCACTTTTGAAA+TGG | - | chr4.2:46711052-46711071 | MS.gene049752:intron | 30.0% |
| !!! | TGCTTCGTTTTTGTTTTTTC+AGG | - | chr4.2:46709832-46709851 | MS.gene049752:CDS | 30.0% |
| !!! | TGTTTGGTTTTAATGTGGAA+TGG | + | chr4.2:46711305-46711324 | None:intergenic | 30.0% |
| AAACCAAACACACTCAATCA+AGG | - | chr4.2:46711315-46711334 | MS.gene049752:intron | 35.0% | |
| AACACACTCAATCAAGGAAA+CGG | - | chr4.2:46711321-46711340 | MS.gene049752:intron | 35.0% | |
| AACATGCCAAAATGCAAATG+AGG | + | chr4.2:46711090-46711109 | None:intergenic | 35.0% | |
| AATATTACCCTGTGCATGAT+TGG | - | chr4.2:46710835-46710854 | MS.gene049752:intron | 35.0% | |
| CACAACAATAAAATGCCACA+TGG | + | chr4.2:46710535-46710554 | None:intergenic | 35.0% | |
| TTTCCTTGATTGAGTGTGTT+TGG | + | chr4.2:46711321-46711340 | None:intergenic | 35.0% | |
| ! | CATGTGGCATTTTATTGTTG+TGG | - | chr4.2:46710533-46710552 | MS.gene049752:intron | 35.0% |
| ! | GCGGTAACATGATTTAAGTT+TGG | + | chr4.2:46710993-46711012 | None:intergenic | 35.0% |
| ! | GGTTACTCTGATGATGAAAT+TGG | - | chr4.2:46710039-46710058 | MS.gene049752:intron | 35.0% |
| !! | TTTGCATTTTGGCATGTTTG+AGG | - | chr4.2:46711092-46711111 | MS.gene049752:intron | 35.0% |
| !!! | GAGTGTGTTTGGTTTTAATG+TGG | + | chr4.2:46711310-46711329 | None:intergenic | 35.0% |
| AAGTGAATCCAATCATGCAC+AGG | + | chr4.2:46710846-46710865 | None:intergenic | 40.0% | |
| AGTGAATCCAATCATGCACA+GGG | + | chr4.2:46710845-46710864 | None:intergenic | 40.0% | |
| ATTGAATTATCAGCAGCAGC+AGG | - | chr4.2:46711708-46711727 | MS.gene049752:intron | 40.0% | |
| CGAAATACTTCTCAGAGTAC+TGG | + | chr4.2:46709715-46709734 | None:intergenic | 40.0% | |
| GGTATTCTCACTGTTGTAAG+AGG | - | chr4.2:46710554-46710573 | MS.gene049752:intron | 40.0% | |
| TCGATGACACTTATGAGTAC+AGG | - | chr4.2:46709732-46709751 | MS.gene049752:CDS | 40.0% | |
| TGCTGCTGCTGATAATTCAA+TGG | + | chr4.2:46711709-46711728 | None:intergenic | 40.0% | |
| TGCTTCAGCTTTGCACTAAA+TGG | + | chr4.2:46711489-46711508 | None:intergenic | 40.0% | |
| TTGGCATGTTTGAGGATTCT+AGG | - | chr4.2:46711100-46711119 | MS.gene049752:intron | 40.0% | |
| ! | GCGATTCCTCATTTGCATTT+TGG | - | chr4.2:46711081-46711100 | MS.gene049752:intron | 40.0% |
| ! | TCAGAGAGAAGTCGATTCTT+GGG | + | chr4.2:46709894-46709913 | None:intergenic | 40.0% |
| ! | TTCAGAGAGAAGTCGATTCT+TGG | + | chr4.2:46709895-46709914 | None:intergenic | 40.0% |
| AGCCACACATTATGCTCTTC+AGG | - | chr4.2:46711680-46711699 | MS.gene049752:intron | 45.0% | |
| CACACATTATGCTCTTCAGG+AGG | - | chr4.2:46711683-46711702 | MS.gene049752:intron | 45.0% | |
| TTGCATAATGAACCCAGCCA+CGG | + | chr4.2:46711648-46711667 | None:intergenic | 45.0% | |
| ! | AAGAGCATAATGTGTGGCTC+AGG | + | chr4.2:46711679-46711698 | None:intergenic | 45.0% |
| ! | CTCCTGAAGAGCATAATGTG+TGG | + | chr4.2:46711685-46711704 | None:intergenic | 45.0% |
| CAGAATGAATGGCGCGCAAT+TGG | - | chr4.2:46711610-46711629 | MS.gene049752:intron | 50.0% | |
| GCATGTTGTTCTTCCTCCAG+AGG | - | chr4.2:46709854-46709873 | MS.gene049752:intron | 50.0% | |
| AGCAACTTCGCAACCTCTGG+AGG | + | chr4.2:46709870-46709889 | None:intergenic | 55.0% | |
| ATTTGACCAGCATGCCCTGC+TGG | + | chr4.2:46711744-46711763 | None:intergenic | 55.0% | |
| GGAAGCAACTTCGCAACCTC+TGG | + | chr4.2:46709873-46709892 | None:intergenic | 55.0% | |
| TTTGACCAGCATGCCCTGCT+GGG | + | chr4.2:46711743-46711762 | None:intergenic | 55.0% | |
| ATTGGAGTGCAGCAGAGCCG+TGG | - | chr4.2:46711628-46711647 | MS.gene049752:intron | 60.0% | |
| TCAGCAGCAGCAGGACAACC+AGG | - | chr4.2:46711717-46711736 | MS.gene049752:CDS | 60.0% | |
| AGTGCAGCAGAGCCGTGGCT+GGG | - | chr4.2:46711633-46711652 | MS.gene049752:intron | 65.0% | |
| CAGGACAACCAGGCCCAGCA+GGG | - | chr4.2:46711727-46711746 | MS.gene049752:CDS | 65.0% | |
| GAGTGCAGCAGAGCCGTGGC+TGG | - | chr4.2:46711632-46711651 | MS.gene049752:intron | 70.0% | |
| GCAGGACAACCAGGCCCAGC+AGG | - | chr4.2:46711726-46711745 | MS.gene049752:CDS | 70.0% | |
| CCAGCATGCCCTGCTGGGCC+TGG | + | chr4.2:46711738-46711757 | None:intergenic | 75.0% | |
| CCAGGCCCAGCAGGGCATGC+TGG | - | chr4.2:46711735-46711754 | MS.gene049752:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 46709701 | 46711765 | 46709701 | ID=MS.gene049752 |
| chr4.2 | mRNA | 46709701 | 46711765 | 46709701 | ID=MS.gene049752.t1;Parent=MS.gene049752 |
| chr4.2 | exon | 46711713 | 46711765 | 46711713 | ID=MS.gene049752.t1.exon1;Parent=MS.gene049752.t1 |
| chr4.2 | CDS | 46711713 | 46711765 | 46711713 | ID=cds.MS.gene049752.t1;Parent=MS.gene049752.t1 |
| chr4.2 | exon | 46711552 | 46711612 | 46711552 | ID=MS.gene049752.t1.exon2;Parent=MS.gene049752.t1 |
| chr4.2 | CDS | 46711552 | 46711612 | 46711552 | ID=cds.MS.gene049752.t1;Parent=MS.gene049752.t1 |
| chr4.2 | exon | 46709701 | 46709853 | 46709701 | ID=MS.gene049752.t1.exon3;Parent=MS.gene049752.t1 |
| chr4.2 | CDS | 46709701 | 46709853 | 46709701 | ID=cds.MS.gene049752.t1;Parent=MS.gene049752.t1 |
| Gene Sequence |
| Protein sequence |
