Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04989.t1 | XP_003600698.1 | 85.6 | 181 | 26 | 0 | 11 | 191 | 1 | 181 | 4.70E-64 | 254.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04989.t1 | G7J8S2 | 85.6 | 181 | 26 | 0 | 11 | 191 | 1 | 181 | 3.4e-64 | 254.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04989 | MS.gene051789 | 0.806892 | 6.60E-50 | -1.69E-46 |
| MS.gene04989 | MS.gene055133 | 0.805452 | 1.33E-49 | -1.69E-46 |
| MS.gene04989 | MS.gene24039 | 0.816903 | 4.35E-52 | -1.69E-46 |
| MS.gene04989 | MS.gene62720 | 0.806204 | 9.21E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGTGGATGGAAGTGCTTTA+AGG | 0.237219 | 3.3:-58751923 | MS.gene04989:CDS |
| TTATAAACTTCAAAGGGTTT+TGG | 0.325679 | 3.3:-58751983 | MS.gene04989:CDS |
| TCAAGAAATGTTGTTGTTTG+TGG | 0.387907 | 3.3:-58751960 | MS.gene04989:CDS |
| TGGCCAAGGTAACTGATCTT+AGG | 0.402243 | 3.3:-58752184 | MS.gene04989:CDS |
| ATTGTCATCCATATGTTTGT+TGG | 0.412328 | 3.3:+58752055 | None:intergenic |
| TTTCTCTCTGAGCTCTCTCA+TGG | 0.423373 | 3.3:-58752390 | MS.gene04989:CDS |
| TCTCTGCTTTCTTGAGGTTG+AGG | 0.424088 | 3.3:+58752353 | None:intergenic |
| CTCAAGTACCAACAAACATA+TGG | 0.441612 | 3.3:-58752063 | MS.gene04989:CDS |
| AATGTTGTTGTTTGTGGTGG+TGG | 0.453933 | 3.3:-58751954 | MS.gene04989:CDS |
| AGTGGATGGAAGTGCTTTAA+GGG | 0.453943 | 3.3:-58751922 | MS.gene04989:CDS |
| AGAATGATGATGGTAATAGA+AGG | 0.459333 | 3.3:-58752025 | MS.gene04989:CDS |
| AAAAGGTTTATAAACTTCAA+AGG | 0.467170 | 3.3:-58751990 | MS.gene04989:CDS |
| AAAGGTTTATAAACTTCAAA+GGG | 0.469059 | 3.3:-58751989 | MS.gene04989:CDS |
| AGCTCATCTCTGCTTTCTTG+AGG | 0.473151 | 3.3:+58752347 | None:intergenic |
| GAAGGATGAATAATAAGAAA+AGG | 0.487756 | 3.3:-58752007 | MS.gene04989:CDS |
| ACGAAGTACAAAGCATTTCA+AGG | 0.503831 | 3.3:-58752106 | MS.gene04989:CDS |
| CCTTGTAAAAGCAGCTGCAT+GGG | 0.506382 | 3.3:-58752321 | MS.gene04989:CDS |
| CACAGGTATAAAAGAGGAAG+TGG | 0.510905 | 3.3:-58752441 | None:intergenic |
| CCCTTGTAAAAGCAGCTGCA+TGG | 0.515732 | 3.3:-58752322 | MS.gene04989:CDS |
| GAGCTCAGAGAGAAAAGCCT+TGG | 0.515979 | 3.3:+58752398 | None:intergenic |
| AGAAATGTTGTTGTTTGTGG+TGG | 0.518716 | 3.3:-58751957 | MS.gene04989:CDS |
| TTTCAGATCTTGAACCATGT+TGG | 0.535997 | 3.3:+58752291 | None:intergenic |
| ATGTTGTTGTTTGTGGTGGT+GGG | 0.536424 | 3.3:-58751953 | MS.gene04989:CDS |
| AACATTCACAGGTATAAAAG+AGG | 0.541854 | 3.3:-58752447 | None:intergenic |
| AGAAGCAATGCGAATGGCCA+AGG | 0.547792 | 3.3:-58752198 | MS.gene04989:CDS |
| TAAAAGCAGCTGCATGGGCA+TGG | 0.548811 | 3.3:-58752316 | MS.gene04989:CDS |
| AACTTGTAACGAGAAGGCCT+CGG | 0.561358 | 3.3:+58752221 | None:intergenic |
| CTTGCTAGATACTTAACTAG+AGG | 0.565930 | 3.3:+58751869 | None:intergenic |
| TCTTAGGTGCAACGGACTTG+AGG | 0.567071 | 3.3:-58752168 | MS.gene04989:CDS |
| TGGTGGTGGGAATGATGTAG+TGG | 0.574409 | 3.3:-58751940 | MS.gene04989:CDS |
| AGGTGCAACGGACTTGAGGT+TGG | 0.576584 | 3.3:-58752164 | MS.gene04989:CDS |
| GGTGGGAATGATGTAGTGGA+TGG | 0.584519 | 3.3:-58751936 | MS.gene04989:CDS |
| AATTGTGCAAAGAATGATGA+TGG | 0.588625 | 3.3:-58752035 | MS.gene04989:CDS |
| GCTTCTAACTTGTAACGAGA+AGG | 0.592890 | 3.3:+58752215 | None:intergenic |
| GCACCTAAGATCAGTTACCT+TGG | 0.604459 | 3.3:+58752181 | None:intergenic |
| GTAACTGATCTTAGGTGCAA+CGG | 0.616466 | 3.3:-58752176 | MS.gene04989:CDS |
| ATCAGTTGTCTGTGAAGCAT+AGG | 0.619879 | 3.3:-58751896 | MS.gene04989:CDS |
| TGTTGGTACTTGAGTCAATG+AGG | 0.623112 | 3.3:+58752072 | None:intergenic |
| CAAGTTAGAAGCAATGCGAA+TGG | 0.644774 | 3.3:-58752204 | MS.gene04989:CDS |
| GCATGGGCATGGTACCAACA+TGG | 0.678579 | 3.3:-58752305 | MS.gene04989:CDS |
| GTTATATAATCATGTCACCA+AGG | 0.679061 | 3.3:-58752415 | MS.gene04989:CDS |
| GTGGATGGAAGTGCTTTAAG+GGG | 0.679790 | 3.3:-58751921 | MS.gene04989:CDS |
| CAAGAACTCGACGTTCACCG+AGG | 0.736761 | 3.3:-58752238 | MS.gene04989:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGGTTTATAAACTTCAA+AGG | - | chr3.3:58752327-58752346 | MS.gene04989:CDS | 20.0% |
| !! | AAAGGTTTATAAACTTCAAA+GGG | - | chr3.3:58752328-58752347 | MS.gene04989:CDS | 20.0% |
| ! | GAAGGATGAATAATAAGAAA+AGG | - | chr3.3:58752310-58752329 | MS.gene04989:CDS | 25.0% |
| !! | TTATAAACTTCAAAGGGTTT+TGG | - | chr3.3:58752334-58752353 | MS.gene04989:CDS | 25.0% |
| AATTGTGCAAAGAATGATGA+TGG | - | chr3.3:58752282-58752301 | MS.gene04989:CDS | 30.0% | |
| ATTGTCATCCATATGTTTGT+TGG | + | chr3.3:58752265-58752284 | None:intergenic | 30.0% | |
| GTTATATAATCATGTCACCA+AGG | - | chr3.3:58751902-58751921 | MS.gene04989:CDS | 30.0% | |
| !! | AGAATGATGATGGTAATAGA+AGG | - | chr3.3:58752292-58752311 | MS.gene04989:CDS | 30.0% |
| !! | TCAAGAAATGTTGTTGTTTG+TGG | - | chr3.3:58752357-58752376 | MS.gene04989:CDS | 30.0% |
| CTCAAGTACCAACAAACATA+TGG | - | chr3.3:58752254-58752273 | MS.gene04989:CDS | 35.0% | |
| TTTCAGATCTTGAACCATGT+TGG | + | chr3.3:58752029-58752048 | None:intergenic | 35.0% | |
| ! | ACGAAGTACAAAGCATTTCA+AGG | - | chr3.3:58752211-58752230 | MS.gene04989:CDS | 35.0% |
| !! | AGAAATGTTGTTGTTTGTGG+TGG | - | chr3.3:58752360-58752379 | MS.gene04989:CDS | 35.0% |
| ATCAGTTGTCTGTGAAGCAT+AGG | - | chr3.3:58752421-58752440 | MS.gene04989:CDS | 40.0% | |
| CAAGTTAGAAGCAATGCGAA+TGG | - | chr3.3:58752113-58752132 | MS.gene04989:CDS | 40.0% | |
| GCTCTCTCATGGAGAAAAAA+AGG | - | chr3.3:58751938-58751957 | MS.gene04989:CDS | 40.0% | |
| GTAACTGATCTTAGGTGCAA+CGG | - | chr3.3:58752141-58752160 | MS.gene04989:CDS | 40.0% | |
| TGTTGGTACTTGAGTCAATG+AGG | + | chr3.3:58752248-58752267 | None:intergenic | 40.0% | |
| ! | GCTTCTAACTTGTAACGAGA+AGG | + | chr3.3:58752105-58752124 | None:intergenic | 40.0% |
| !! | AATGTTGTTGTTTGTGGTGG+TGG | - | chr3.3:58752363-58752382 | MS.gene04989:CDS | 40.0% |
| !! | AGTGGATGGAAGTGCTTTAA+GGG | - | chr3.3:58752395-58752414 | MS.gene04989:CDS | 40.0% |
| !! | ATGTTGTTGTTTGTGGTGGT+GGG | - | chr3.3:58752364-58752383 | MS.gene04989:CDS | 40.0% |
| !! | TAGTGGATGGAAGTGCTTTA+AGG | - | chr3.3:58752394-58752413 | MS.gene04989:CDS | 40.0% |
| AACTTGTAACGAGAAGGCCT+CGG | + | chr3.3:58752099-58752118 | None:intergenic | 45.0% | |
| AGCTCATCTCTGCTTTCTTG+AGG | + | chr3.3:58751973-58751992 | None:intergenic | 45.0% | |
| CCTTGTAAAAGCAGCTGCAT+GGG | - | chr3.3:58751996-58752015 | MS.gene04989:CDS | 45.0% | |
| GCACCTAAGATCAGTTACCT+TGG | + | chr3.3:58752139-58752158 | None:intergenic | 45.0% | |
| TCTCTGCTTTCTTGAGGTTG+AGG | + | chr3.3:58751967-58751986 | None:intergenic | 45.0% | |
| TGGCCAAGGTAACTGATCTT+AGG | - | chr3.3:58752133-58752152 | MS.gene04989:CDS | 45.0% | |
| TTTCTCTCTGAGCTCTCTCA+TGG | - | chr3.3:58751927-58751946 | MS.gene04989:CDS | 45.0% | |
| !! | GTGGATGGAAGTGCTTTAAG+GGG | - | chr3.3:58752396-58752415 | MS.gene04989:CDS | 45.0% |
| !!! | CCATGCAGCTGCTTTTACAA+GGG | + | chr3.3:58751998-58752017 | None:intergenic | 45.0% |
| AGAAGCAATGCGAATGGCCA+AGG | - | chr3.3:58752119-58752138 | MS.gene04989:CDS | 50.0% | |
| CCCTTGTAAAAGCAGCTGCA+TGG | - | chr3.3:58751995-58752014 | MS.gene04989:CDS | 50.0% | |
| GAGCTCAGAGAGAAAAGCCT+TGG | + | chr3.3:58751922-58751941 | None:intergenic | 50.0% | |
| TAAAAGCAGCTGCATGGGCA+TGG | - | chr3.3:58752001-58752020 | MS.gene04989:CDS | 50.0% | |
| ! | GGTGGGAATGATGTAGTGGA+TGG | - | chr3.3:58752381-58752400 | MS.gene04989:CDS | 50.0% |
| ! | TCTTAGGTGCAACGGACTTG+AGG | - | chr3.3:58752149-58752168 | MS.gene04989:CDS | 50.0% |
| !! | TGGTGGTGGGAATGATGTAG+TGG | - | chr3.3:58752377-58752396 | MS.gene04989:CDS | 50.0% |
| !!! | CCCATGCAGCTGCTTTTACA+AGG | + | chr3.3:58751999-58752018 | None:intergenic | 50.0% |
| CAAGAACTCGACGTTCACCG+AGG | - | chr3.3:58752079-58752098 | MS.gene04989:CDS | 55.0% | |
| GCATGGGCATGGTACCAACA+TGG | - | chr3.3:58752012-58752031 | MS.gene04989:CDS | 55.0% | |
| ! | AGGTGCAACGGACTTGAGGT+TGG | - | chr3.3:58752153-58752172 | MS.gene04989:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 58751881 | 58752458 | 58751881 | ID=MS.gene04989 |
| chr3.3 | mRNA | 58751881 | 58752458 | 58751881 | ID=MS.gene04989.t1;Parent=MS.gene04989 |
| chr3.3 | exon | 58751881 | 58752458 | 58751881 | ID=MS.gene04989.t1.exon1;Parent=MS.gene04989.t1 |
| chr3.3 | CDS | 58751881 | 58752458 | 58751881 | ID=cds.MS.gene04989.t1;Parent=MS.gene04989.t1 |
| Gene Sequence |
| Protein sequence |
