Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24039.t1 | XP_013468360.1 | 94.5 | 55 | 3 | 0 | 1 | 55 | 1 | 55 | 1.60E-20 | 109 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24039.t1 | A0A072VKU7 | 94.5 | 55 | 3 | 0 | 1 | 55 | 1 | 55 | 1.1e-20 | 109.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene24039 | 0.805114 | 1.56E-49 | -1.69E-46 |
| MS.gene049341 | MS.gene24039 | 0.812796 | 3.54E-51 | -1.69E-46 |
| MS.gene049382 | MS.gene24039 | 0.829641 | 4.59E-55 | -1.69E-46 |
| MS.gene04989 | MS.gene24039 | 0.816903 | 4.35E-52 | -1.69E-46 |
| MS.gene050020 | MS.gene24039 | 0.838727 | 2.42E-57 | -1.69E-46 |
| MS.gene050128 | MS.gene24039 | 0.820982 | 5.14E-53 | -1.69E-46 |
| MS.gene050584 | MS.gene24039 | 0.885259 | 9.49E-72 | -1.69E-46 |
| MS.gene050585 | MS.gene24039 | 0.839586 | 1.45E-57 | -1.69E-46 |
| MS.gene050586 | MS.gene24039 | 0.823451 | 1.38E-53 | -1.69E-46 |
| MS.gene050762 | MS.gene24039 | 0.804789 | 1.83E-49 | -1.69E-46 |
| MS.gene050763 | MS.gene24039 | 0.80588 | 1.08E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene24039 | 0.81819 | 2.23E-52 | -1.69E-46 |
| MS.gene050815 | MS.gene24039 | 0.810802 | 9.62E-51 | -1.69E-46 |
| MS.gene051626 | MS.gene24039 | 0.857956 | 1.15E-62 | -1.69E-46 |
| MS.gene051789 | MS.gene24039 | 0.906788 | 1.02E-80 | -1.69E-46 |
| MS.gene052178 | MS.gene24039 | 0.811135 | 8.14E-51 | -1.69E-46 |
| MS.gene052286 | MS.gene24039 | 0.803246 | 3.82E-49 | -1.69E-46 |
| MS.gene052337 | MS.gene24039 | 0.814579 | 1.43E-51 | -1.69E-46 |
| MS.gene052338 | MS.gene24039 | 0.823031 | 1.72E-53 | -1.69E-46 |
| MS.gene052967 | MS.gene24039 | 0.855305 | 6.90E-62 | -1.69E-46 |
| MS.gene053074 | MS.gene24039 | 0.906612 | 1.23E-80 | -1.69E-46 |
| MS.gene053204 | MS.gene24039 | 0.832237 | 1.06E-55 | -1.69E-46 |
| MS.gene053563 | MS.gene24039 | 0.807314 | 5.37E-50 | -1.69E-46 |
| MS.gene053630 | MS.gene24039 | 0.800446 | 1.44E-48 | -1.69E-46 |
| MS.gene054184 | MS.gene24039 | 0.842672 | 2.24E-58 | -1.69E-46 |
| MS.gene054185 | MS.gene24039 | 0.847071 | 1.46E-59 | -1.69E-46 |
| MS.gene05456 | MS.gene24039 | 0.833752 | 4.45E-56 | -1.69E-46 |
| MS.gene054596 | MS.gene24039 | 0.850889 | 1.27E-60 | -1.69E-46 |
| MS.gene055133 | MS.gene24039 | 0.873461 | 1.44E-67 | -1.69E-46 |
| MS.gene055207 | MS.gene24039 | 0.871034 | 9.29E-67 | -1.69E-46 |
| MS.gene056069 | MS.gene24039 | -0.813136 | 2.98E-51 | -1.69E-46 |
| MS.gene056760 | MS.gene24039 | 0.808382 | 3.18E-50 | -1.69E-46 |
| MS.gene057057 | MS.gene24039 | 0.812289 | 4.57E-51 | -1.69E-46 |
| MS.gene057393 | MS.gene24039 | 0.800726 | 1.26E-48 | -1.69E-46 |
| MS.gene057754 | MS.gene24039 | 0.836255 | 1.04E-56 | -1.69E-46 |
| MS.gene057794 | MS.gene24039 | 0.804375 | 2.23E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene24039 | 0.826984 | 2.01E-54 | -1.69E-46 |
| MS.gene058906 | MS.gene24039 | 0.816175 | 6.33E-52 | -1.69E-46 |
| MS.gene059006 | MS.gene24039 | 0.888622 | 5.01E-73 | -1.69E-46 |
| MS.gene059124 | MS.gene24039 | 0.802016 | 6.85E-49 | -1.69E-46 |
| MS.gene059228 | MS.gene24039 | 0.825028 | 5.86E-54 | -1.69E-46 |
| MS.gene060476 | MS.gene24039 | 0.829835 | 4.12E-55 | -1.69E-46 |
| MS.gene061113 | MS.gene24039 | 0.81598 | 7.00E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24039.t1 | MTR_1g069085 | 95.918 | 49 | 2 | 0 | 1 | 49 | 1 | 49 | 1.93e-27 | 98.2 |
| MS.gene24039.t1 | MTR_7g099670 | 62.264 | 53 | 18 | 2 | 1 | 52 | 1 | 52 | 3.06e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 55 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGGTGCTGGTGGACTTCC+TGG | 0.194284 | 1.3:+48347892 | MS.gene24039:CDS |
| CTTGGTGGTGCTGGTGGCTT+TGG | 0.225781 | 1.3:+48347916 | MS.gene24039:CDS |
| CTTGGCGGTGCTGGTGGACT+TGG | 0.262306 | 1.3:+48347952 | MS.gene24039:CDS |
| GGTGATGCTGGTGGTGGACT+TGG | 0.309365 | 1.3:+48347826 | MS.gene24039:CDS |
| CTCCCCGGACTTGGCGGTGC+TGG | 0.314858 | 1.3:+48347943 | MS.gene24039:CDS |
| CTTGGAGGACTTGGTGGTGC+TGG | 0.318812 | 1.3:+48347970 | MS.gene24039:CDS |
| GGTAACAATGGAATGCCTTT+TGG | 0.333736 | 1.3:+48347781 | MS.gene24039:CDS |
| TTTGGTGGTGCTGGTGATGC+TGG | 0.337285 | 1.3:+48347814 | MS.gene24039:CDS |
| CTTCCTGGACTTGGTGGTGC+TGG | 0.351425 | 1.3:+48347907 | MS.gene24039:CDS |
| TTTGGTGGACTCCCCGGACT+TGG | 0.357923 | 1.3:+48347934 | MS.gene24039:CDS |
| TCCACCAGCACCGCCAAGTC+CGG | 0.370119 | 1.3:-48347947 | None:intergenic |
| GGTGTAGGCTTTGGTGGTGC+TGG | 0.371956 | 1.3:+48347805 | MS.gene24039:CDS |
| GGAATCGGTGGTGGTGGTGC+TGG | 0.378134 | 1.3:+48347880 | MS.gene24039:CDS |
| GGACTTGGTGGTGCTGGTGG+TGG | 0.379039 | 1.3:+48347976 | MS.gene24039:CDS |
| GCTGGTGGACTTGGAGGACT+TGG | 0.388396 | 1.3:+48347961 | MS.gene24039:CDS |
| GCTGGTGGACTTCCTGGACT+TGG | 0.401091 | 1.3:+48347898 | MS.gene24039:CDS |
| CCTGGTGGACTCGGAGGAAT+CGG | 0.402378 | 1.3:+48347865 | MS.gene24039:CDS |
| GGTGCTGGTGATGCTGGTGG+TGG | 0.408529 | 1.3:+48347820 | MS.gene24039:CDS |
| GGTGGCGGTCCTGGTGGACT+CGG | 0.420053 | 1.3:+48347856 | MS.gene24039:CDS |
| GGTGGTGGACTTGGAGGACT+AGG | 0.420340 | 1.3:+48347835 | MS.gene24039:CDS |
| GGTGGTGCTGGTGGCTTTGG+TGG | 0.420680 | 1.3:+48347919 | MS.gene24039:CDS |
| CCACCAGCACCGCCAAGTCC+GGG | 0.421149 | 1.3:-48347946 | None:intergenic |
| GGAGGACTAGGTGGCGGTCC+TGG | 0.432791 | 1.3:+48347847 | MS.gene24039:CDS |
| CTCGGAGGAATCGGTGGTGG+TGG | 0.446365 | 1.3:+48347874 | MS.gene24039:CDS |
| TAACTTGTCGCTCAAGGTTG+AGG | 0.451174 | 1.3:-48348006 | None:intergenic |
| CCCGGACTTGGCGGTGCTGG+TGG | 0.458285 | 1.3:+48347946 | MS.gene24039:CDS |
| TTTGGAGGTGTAGGCTTTGG+TGG | 0.458483 | 1.3:+48347799 | MS.gene24039:CDS |
| AATGTCTAAGTGGTGCATTT+TGG | 0.458799 | 1.3:+48347645 | None:intergenic |
| GGCGGTGCTGGTGGACTTGG+AGG | 0.462449 | 1.3:+48347955 | MS.gene24039:CDS |
| GATGCTGGTGGTGGACTTGG+AGG | 0.463937 | 1.3:+48347829 | MS.gene24039:CDS |
| GGTGGACTTGGAGGACTTGG+TGG | 0.474829 | 1.3:+48347964 | MS.gene24039:CDS |
| GGTGGACTCCCCGGACTTGG+CGG | 0.480200 | 1.3:+48347937 | MS.gene24039:CDS |
| GGTGGCTTTGGTGGACTCCC+CGG | 0.490321 | 1.3:+48347928 | MS.gene24039:CDS |
| GGACTCGGAGGAATCGGTGG+TGG | 0.495212 | 1.3:+48347871 | MS.gene24039:CDS |
| AAGAATTACATGACTGGAGC+TGG | 0.500445 | 1.3:+48347742 | MS.gene24039:CDS |
| ATCGGTGGTGGTGGTGCTGG+TGG | 0.500516 | 1.3:+48347883 | MS.gene24039:CDS |
| GGTGGACTTCCTGGACTTGG+TGG | 0.506089 | 1.3:+48347901 | MS.gene24039:CDS |
| AGCTGGAGAAGATGGTTTGA+AGG | 0.514012 | 1.3:+48347714 | MS.gene24039:CDS |
| GGAGGACTTGGTGGTGCTGG+TGG | 0.518986 | 1.3:+48347973 | MS.gene24039:CDS |
| CCGATTCCTCCGAGTCCACC+AGG | 0.519351 | 1.3:-48347865 | None:intergenic |
| AATGTGCCAGCTGGAGAAGA+TGG | 0.524167 | 1.3:+48347706 | MS.gene24039:CDS |
| CCTGGACTTGGTGGTGCTGG+TGG | 0.528901 | 1.3:+48347910 | MS.gene24039:CDS |
| TTCAAACCATCTTCTCCAGC+TGG | 0.531041 | 1.3:-48347712 | None:intergenic |
| CCACCAGCACCACCAAGTCC+AGG | 0.542661 | 1.3:-48347910 | None:intergenic |
| CCAAAGCCTACACCTCCAAA+AGG | 0.544740 | 1.3:-48347796 | None:intergenic |
| GATCAGAAGAATTACATGAC+TGG | 0.553149 | 1.3:+48347736 | MS.gene24039:CDS |
| GGTGGTGCTGGTGATGCTGG+TGG | 0.553318 | 1.3:+48347817 | MS.gene24039:CDS |
| AATGCAAGAAATGTGCCAGC+TGG | 0.554804 | 1.3:+48347697 | MS.gene24039:CDS |
| GGTGGACTCGGAGGAATCGG+TGG | 0.566209 | 1.3:+48347868 | MS.gene24039:CDS |
| GGACTAGGTGGCGGTCCTGG+TGG | 0.568473 | 1.3:+48347850 | MS.gene24039:CDS |
| CACCAGCACCGCCAAGTCCG+GGG | 0.580869 | 1.3:-48347945 | None:intergenic |
| GGCGGTCCTGGTGGACTCGG+AGG | 0.589973 | 1.3:+48347859 | MS.gene24039:CDS |
| GGACTTGGAGGACTAGGTGG+CGG | 0.603551 | 1.3:+48347841 | MS.gene24039:CDS |
| GGTGGACTTGGAGGACTAGG+TGG | 0.622805 | 1.3:+48347838 | MS.gene24039:CDS |
| TGAAGAGAGAAATGTCTAAG+TGG | 0.655500 | 1.3:+48347635 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GATCAGAAGAATTACATGAC+TGG | + | chr1.3:48347736-48347755 | MS.gene24039:CDS | 35.0% | |
| !!! | TTTTCAGGCATTGGTAACAA+TGG | + | chr1.3:48347769-48347788 | MS.gene24039:CDS | 35.0% |
| AAGAATTACATGACTGGAGC+TGG | + | chr1.3:48347742-48347761 | MS.gene24039:CDS | 40.0% | |
| ! | AACAATGGAATGCCTTTTGG+AGG | + | chr1.3:48347784-48347803 | MS.gene24039:CDS | 40.0% |
| ! | GGTAACAATGGAATGCCTTT+TGG | + | chr1.3:48347781-48347800 | MS.gene24039:CDS | 40.0% |
| !!! | GTGGTGCATTTTGGTTGTTT+TGG | + | chr1.3:48347654-48347673 | MS.gene24039:CDS | 40.0% |
| AATGCAAGAAATGTGCCAGC+TGG | + | chr1.3:48347697-48347716 | MS.gene24039:CDS | 45.0% | |
| AGCTGGAGAAGATGGTTTGA+AGG | + | chr1.3:48347714-48347733 | MS.gene24039:CDS | 45.0% | |
| TTCAAACCATCTTCTCCAGC+TGG | - | chr1.3:48347715-48347734 | None:intergenic | 45.0% | |
| !!! | ACTGGAGCTGGTGTTTTTTC+AGG | + | chr1.3:48347754-48347773 | MS.gene24039:CDS | 45.0% |
| !!! | GCTGGTGTTTTTTCAGGCAT+TGG | + | chr1.3:48347760-48347779 | MS.gene24039:CDS | 45.0% |
| AATGTGCCAGCTGGAGAAGA+TGG | + | chr1.3:48347706-48347725 | MS.gene24039:CDS | 50.0% | |
| CCAAAGCCTACACCTCCAAA+AGG | - | chr1.3:48347799-48347818 | None:intergenic | 50.0% | |
| ! | TTTGGAGGTGTAGGCTTTGG+TGG | + | chr1.3:48347799-48347818 | MS.gene24039:CDS | 50.0% |
| !! | CCTTTTGGAGGTGTAGGCTT+TGG | + | chr1.3:48347796-48347815 | MS.gene24039:CDS | 50.0% |
| !! | GGAATGCCTTTTGGAGGTGT+AGG | + | chr1.3:48347790-48347809 | MS.gene24039:CDS | 50.0% |
| !!! | GGTTGTTTTGGCTCTTGCTG+TGG | + | chr1.3:48347666-48347685 | MS.gene24039:CDS | 50.0% |
| !! | TTTGGTGGTGCTGGTGATGC+TGG | + | chr1.3:48347814-48347833 | MS.gene24039:CDS | 55.0% |
| GGACTTGGAGGACTAGGTGG+CGG | + | chr1.3:48347841-48347860 | MS.gene24039:CDS | 60.0% | |
| ! | GGTGGACTTCCTGGACTTGG+TGG | + | chr1.3:48347901-48347920 | MS.gene24039:CDS | 60.0% |
| ! | GGTGGACTTGGAGGACTAGG+TGG | + | chr1.3:48347838-48347857 | MS.gene24039:CDS | 60.0% |
| ! | GGTGGACTTGGAGGACTTGG+TGG | + | chr1.3:48347964-48347983 | MS.gene24039:CDS | 60.0% |
| !! | CCTGGTGGACTCGGAGGAAT+CGG | + | chr1.3:48347865-48347884 | MS.gene24039:CDS | 60.0% |
| !! | CTTCCTGGACTTGGTGGTGC+TGG | + | chr1.3:48347907-48347926 | MS.gene24039:CDS | 60.0% |
| !! | CTTGGAGGACTTGGTGGTGC+TGG | + | chr1.3:48347970-48347989 | MS.gene24039:CDS | 60.0% |
| !! | CTTGGTGGTGCTGGTGGCTT+TGG | + | chr1.3:48347916-48347935 | MS.gene24039:CDS | 60.0% |
| !! | GATGCTGGTGGTGGACTTGG+AGG | + | chr1.3:48347829-48347848 | MS.gene24039:CDS | 60.0% |
| !! | GCTGGTGGACTTCCTGGACT+TGG | + | chr1.3:48347898-48347917 | MS.gene24039:CDS | 60.0% |
| !! | GCTGGTGGACTTGGAGGACT+TGG | + | chr1.3:48347961-48347980 | MS.gene24039:CDS | 60.0% |
| !! | GGTGATGCTGGTGGTGGACT+TGG | + | chr1.3:48347826-48347845 | MS.gene24039:CDS | 60.0% |
| !! | GGTGGTGGACTTGGAGGACT+AGG | + | chr1.3:48347835-48347854 | MS.gene24039:CDS | 60.0% |
| !! | GGTGTAGGCTTTGGTGGTGC+TGG | + | chr1.3:48347805-48347824 | MS.gene24039:CDS | 60.0% |
| !! | TTTGGTGGACTCCCCGGACT+TGG | + | chr1.3:48347934-48347953 | MS.gene24039:CDS | 60.0% |
| CCACCAGCACCACCAAGTCC+AGG | - | chr1.3:48347913-48347932 | None:intergenic | 65.0% | |
| CCGATTCCTCCGAGTCCACC+AGG | - | chr1.3:48347868-48347887 | None:intergenic | 65.0% | |
| GGACTCGGAGGAATCGGTGG+TGG | + | chr1.3:48347871-48347890 | MS.gene24039:CDS | 65.0% | |
| TCCACCAGCACCGCCAAGTC+CGG | - | chr1.3:48347950-48347969 | None:intergenic | 65.0% | |
| ! | CTCGGAGGAATCGGTGGTGG+TGG | + | chr1.3:48347874-48347893 | MS.gene24039:CDS | 65.0% |
| ! | GGTGGACTCGGAGGAATCGG+TGG | + | chr1.3:48347868-48347887 | MS.gene24039:CDS | 65.0% |
| !! | ATCGGTGGTGGTGGTGCTGG+TGG | + | chr1.3:48347883-48347902 | MS.gene24039:CDS | 65.0% |
| !! | CCTGGACTTGGTGGTGCTGG+TGG | + | chr1.3:48347910-48347929 | MS.gene24039:CDS | 65.0% |
| !! | CTTGGCGGTGCTGGTGGACT+TGG | + | chr1.3:48347952-48347971 | MS.gene24039:CDS | 65.0% |
| !! | GGAATCGGTGGTGGTGGTGC+TGG | + | chr1.3:48347880-48347899 | MS.gene24039:CDS | 65.0% |
| !! | GGACTTGGTGGTGCTGGTGG+TGG | + | chr1.3:48347913-48347932 | MS.gene24039:CDS | 65.0% |
| !! | GGAGGACTTGGTGGTGCTGG+TGG | + | chr1.3:48347973-48347992 | MS.gene24039:CDS | 65.0% |
| !! | GGTGCTGGTGATGCTGGTGG+TGG | + | chr1.3:48347820-48347839 | MS.gene24039:CDS | 65.0% |
| !! | GGTGGCTTTGGTGGACTCCC+CGG | + | chr1.3:48347928-48347947 | MS.gene24039:CDS | 65.0% |
| !! | GGTGGTGCTGGTGATGCTGG+TGG | + | chr1.3:48347817-48347836 | MS.gene24039:CDS | 65.0% |
| !! | GGTGGTGCTGGTGGACTTCC+TGG | + | chr1.3:48347892-48347911 | MS.gene24039:CDS | 65.0% |
| !! | GGTGGTGCTGGTGGCTTTGG+TGG | + | chr1.3:48347919-48347938 | MS.gene24039:CDS | 65.0% |
| CACCAGCACCGCCAAGTCCG+GGG | - | chr1.3:48347948-48347967 | None:intergenic | 70.0% | |
| CCACCAGCACCGCCAAGTCC+GGG | - | chr1.3:48347949-48347968 | None:intergenic | 70.0% | |
| ! | GGACTAGGTGGCGGTCCTGG+TGG | + | chr1.3:48347850-48347869 | MS.gene24039:CDS | 70.0% |
| ! | GGAGGACTAGGTGGCGGTCC+TGG | + | chr1.3:48347847-48347866 | MS.gene24039:CDS | 70.0% |
| ! | GGTGGACTCCCCGGACTTGG+CGG | + | chr1.3:48347937-48347956 | MS.gene24039:CDS | 70.0% |
| !! | GGCGGTGCTGGTGGACTTGG+AGG | + | chr1.3:48347955-48347974 | MS.gene24039:CDS | 70.0% |
| !! | GGTGGCGGTCCTGGTGGACT+CGG | + | chr1.3:48347856-48347875 | MS.gene24039:CDS | 70.0% |
| CTCCCCGGACTTGGCGGTGC+TGG | + | chr1.3:48347943-48347962 | MS.gene24039:CDS | 75.0% | |
| ! | CCCGGACTTGGCGGTGCTGG+TGG | + | chr1.3:48347946-48347965 | MS.gene24039:CDS | 75.0% |
| !! | GGCGGTCCTGGTGGACTCGG+AGG | + | chr1.3:48347859-48347878 | MS.gene24039:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 48347646 | 48348017 | 48347646 | ID=MS.gene24039 |
| chr1.3 | mRNA | 48347646 | 48348017 | 48347646 | ID=MS.gene24039.t1;Parent=MS.gene24039 |
| chr1.3 | exon | 48347646 | 48348017 | 48347646 | ID=MS.gene24039.t1.exon1;Parent=MS.gene24039.t1 |
| chr1.3 | CDS | 48347646 | 48348017 | 48347646 | ID=cds.MS.gene24039.t1;Parent=MS.gene24039.t1 |
| Gene Sequence |
| Protein sequence |
