Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62720.t1 | AFK36884.1 | 96.2 | 239 | 9 | 0 | 1 | 239 | 119 | 357 | 2.90E-79 | 305.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62720.t1 | Q9LXF1 | 31.9 | 260 | 104 | 7 | 1 | 239 | 119 | 326 | 2.8e-14 | 80.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62720.t1 | I3S9E2 | 96.2 | 239 | 9 | 0 | 1 | 239 | 119 | 357 | 2.1e-79 | 305.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049151 | MS.gene62720 | 0.808761 | 2.64E-50 | -1.69E-46 |
| MS.gene049260 | MS.gene62720 | 0.847425 | 1.17E-59 | -1.69E-46 |
| MS.gene049297 | MS.gene62720 | 0.80891 | 2.46E-50 | -1.69E-46 |
| MS.gene049341 | MS.gene62720 | 0.816951 | 4.24E-52 | -1.69E-46 |
| MS.gene049382 | MS.gene62720 | 0.849422 | 3.27E-60 | -1.69E-46 |
| MS.gene049756 | MS.gene62720 | 0.800596 | 1.34E-48 | -1.69E-46 |
| MS.gene049758 | MS.gene62720 | 0.82362 | 1.26E-53 | -1.69E-46 |
| MS.gene04989 | MS.gene62720 | 0.806204 | 9.21E-50 | -1.69E-46 |
| MS.gene049915 | MS.gene62720 | 0.803392 | 3.56E-49 | -1.69E-46 |
| MS.gene049979 | MS.gene62720 | 0.814659 | 1.38E-51 | -1.69E-46 |
| MS.gene050020 | MS.gene62720 | 0.878456 | 2.77E-69 | -1.69E-46 |
| MS.gene050128 | MS.gene62720 | 0.816398 | 5.65E-52 | -1.69E-46 |
| MS.gene050226 | MS.gene62720 | 0.807328 | 5.33E-50 | -1.69E-46 |
| MS.gene050438 | MS.gene62720 | 0.826061 | 3.34E-54 | -1.69E-46 |
| MS.gene050584 | MS.gene62720 | 0.873553 | 1.35E-67 | -1.69E-46 |
| MS.gene050585 | MS.gene62720 | 0.840666 | 7.58E-58 | -1.69E-46 |
| MS.gene050586 | MS.gene62720 | 0.868494 | 6.26E-66 | -1.69E-46 |
| MS.gene050587 | MS.gene62720 | 0.806546 | 7.81E-50 | -1.69E-46 |
| MS.gene050762 | MS.gene62720 | 0.834081 | 3.68E-56 | -1.69E-46 |
| MS.gene050763 | MS.gene62720 | 0.834792 | 2.44E-56 | -1.69E-46 |
| MS.gene050807 | MS.gene62720 | 0.857754 | 1.32E-62 | -1.69E-46 |
| MS.gene050808 | MS.gene62720 | 0.825073 | 5.72E-54 | -1.69E-46 |
| MS.gene050815 | MS.gene62720 | 0.84343 | 1.41E-58 | -1.69E-46 |
| MS.gene051243 | MS.gene62720 | 0.82238 | 2.44E-53 | -1.69E-46 |
| MS.gene051626 | MS.gene62720 | 0.889908 | 1.59E-73 | -1.69E-46 |
| MS.gene051662 | MS.gene62720 | 0.814698 | 1.35E-51 | -1.69E-46 |
| MS.gene051663 | MS.gene62720 | 0.813455 | 2.54E-51 | -1.69E-46 |
| MS.gene051789 | MS.gene62720 | 0.936676 | 1.19E-97 | -1.69E-46 |
| MS.gene051860 | MS.gene62720 | 0.802806 | 4.71E-49 | -1.69E-46 |
| MS.gene052142 | MS.gene62720 | 0.822195 | 2.70E-53 | -1.69E-46 |
| MS.gene052178 | MS.gene62720 | 0.831001 | 2.14E-55 | -1.69E-46 |
| MS.gene052286 | MS.gene62720 | 0.826994 | 2.00E-54 | -1.69E-46 |
| MS.gene052302 | MS.gene62720 | 0.815493 | 8.99E-52 | -1.69E-46 |
| MS.gene052337 | MS.gene62720 | 0.818742 | 1.67E-52 | -1.69E-46 |
| MS.gene052376 | MS.gene62720 | 0.822297 | 2.55E-53 | -1.69E-46 |
| MS.gene052665 | MS.gene62720 | 0.846831 | 1.70E-59 | -1.69E-46 |
| MS.gene052856 | MS.gene62720 | 0.80923 | 2.10E-50 | -1.69E-46 |
| MS.gene052858 | MS.gene62720 | 0.806176 | 9.34E-50 | -1.69E-46 |
| MS.gene052860 | MS.gene62720 | 0.818431 | 1.97E-52 | -1.69E-46 |
| MS.gene052967 | MS.gene62720 | 0.884514 | 1.80E-71 | -1.69E-46 |
| MS.gene053074 | MS.gene62720 | 0.914043 | 3.04E-84 | -1.69E-46 |
| MS.gene053184 | MS.gene62720 | 0.80674 | 7.10E-50 | -1.69E-46 |
| MS.gene053204 | MS.gene62720 | 0.841494 | 4.59E-58 | -1.69E-46 |
| MS.gene053563 | MS.gene62720 | 0.826697 | 2.35E-54 | -1.69E-46 |
| MS.gene053630 | MS.gene62720 | 0.860626 | 1.81E-63 | -1.69E-46 |
| MS.gene053836 | MS.gene62720 | 0.821026 | 5.02E-53 | -1.69E-46 |
| MS.gene054184 | MS.gene62720 | 0.865695 | 4.90E-65 | -1.69E-46 |
| MS.gene054185 | MS.gene62720 | 0.870085 | 1.90E-66 | -1.69E-46 |
| MS.gene054214 | MS.gene62720 | 0.816151 | 6.41E-52 | -1.69E-46 |
| MS.gene054379 | MS.gene62720 | 0.822917 | 1.83E-53 | -1.69E-46 |
| MS.gene05456 | MS.gene62720 | 0.867293 | 1.52E-65 | -1.69E-46 |
| MS.gene054596 | MS.gene62720 | 0.884064 | 2.64E-71 | -1.69E-46 |
| MS.gene054667 | MS.gene62720 | 0.815861 | 7.44E-52 | -1.69E-46 |
| MS.gene054942 | MS.gene62720 | 0.810071 | 1.38E-50 | -1.69E-46 |
| MS.gene055085 | MS.gene62720 | 0.802621 | 5.14E-49 | -1.69E-46 |
| MS.gene055133 | MS.gene62720 | 0.861706 | 8.49E-64 | -1.69E-46 |
| MS.gene055207 | MS.gene62720 | 0.891721 | 3.07E-74 | -1.69E-46 |
| MS.gene055359 | MS.gene62720 | 0.813518 | 2.46E-51 | -1.69E-46 |
| MS.gene055604 | MS.gene62720 | 0.805723 | 1.16E-49 | -1.69E-46 |
| MS.gene056069 | MS.gene62720 | -0.841662 | 4.15E-58 | -1.69E-46 |
| MS.gene056380 | MS.gene62720 | 0.813633 | 2.32E-51 | -1.69E-46 |
| MS.gene056552 | MS.gene62720 | 0.803892 | 2.81E-49 | -1.69E-46 |
| MS.gene056589 | MS.gene62720 | 0.81034 | 1.21E-50 | -1.69E-46 |
| MS.gene056760 | MS.gene62720 | 0.843754 | 1.15E-58 | -1.69E-46 |
| MS.gene056875 | MS.gene62720 | 0.803789 | 2.95E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene62720 | 0.80536 | 1.39E-49 | -1.69E-46 |
| MS.gene057260 | MS.gene62720 | 0.831502 | 1.61E-55 | -1.69E-46 |
| MS.gene057393 | MS.gene62720 | 0.815282 | 1.00E-51 | -1.69E-46 |
| MS.gene057750 | MS.gene62720 | 0.812513 | 4.08E-51 | -1.69E-46 |
| MS.gene057754 | MS.gene62720 | 0.863249 | 2.85E-64 | -1.69E-46 |
| MS.gene057869 | MS.gene62720 | 0.801212 | 1.00E-48 | -1.69E-46 |
| MS.gene058168 | MS.gene62720 | 0.883117 | 5.89E-71 | -1.69E-46 |
| MS.gene058182 | MS.gene62720 | 0.820377 | 7.08E-53 | -1.69E-46 |
| MS.gene058906 | MS.gene62720 | 0.837294 | 5.66E-57 | -1.69E-46 |
| MS.gene058982 | MS.gene62720 | 0.819896 | 9.13E-53 | -1.69E-46 |
| MS.gene059006 | MS.gene62720 | 0.913197 | 8.14E-84 | -1.69E-46 |
| MS.gene059124 | MS.gene62720 | 0.860991 | 1.40E-63 | -1.69E-46 |
| MS.gene059228 | MS.gene62720 | 0.822275 | 2.58E-53 | -1.69E-46 |
| MS.gene059421 | MS.gene62720 | 0.802102 | 6.58E-49 | -1.69E-46 |
| MS.gene059937 | MS.gene62720 | 0.824995 | 5.97E-54 | -1.69E-46 |
| MS.gene060320 | MS.gene62720 | 0.810895 | 9.18E-51 | -1.69E-46 |
| MS.gene060404 | MS.gene62720 | 0.804542 | 2.05E-49 | -1.69E-46 |
| MS.gene060470 | MS.gene62720 | 0.824257 | 8.90E-54 | -1.69E-46 |
| MS.gene060476 | MS.gene62720 | 0.855968 | 4.42E-62 | -1.69E-46 |
| MS.gene060480 | MS.gene62720 | 0.805354 | 1.39E-49 | -1.69E-46 |
| MS.gene06065 | MS.gene62720 | 0.801099 | 1.06E-48 | -1.69E-46 |
| MS.gene060803 | MS.gene62720 | 0.819745 | 9.88E-53 | -1.69E-46 |
| MS.gene060998 | MS.gene62720 | 0.802439 | 5.61E-49 | -1.69E-46 |
| MS.gene061113 | MS.gene62720 | 0.83939 | 1.63E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62720.t1 | MTR_6g012690 | 93.305 | 239 | 16 | 0 | 1 | 239 | 119 | 357 | 2.81e-133 | 379 |
| MS.gene62720.t1 | MTR_7g011170 | 54.839 | 124 | 28 | 5 | 132 | 239 | 198 | 309 | 8.57e-24 | 97.4 |
| MS.gene62720.t1 | MTR_7g011170 | 75.000 | 56 | 11 | 1 | 1 | 56 | 119 | 171 | 1.33e-19 | 85.9 |
| MS.gene62720.t1 | MTR_4g082290 | 63.333 | 60 | 21 | 1 | 1 | 59 | 119 | 178 | 2.63e-16 | 77.8 |
| MS.gene62720.t1 | MTR_4g082230 | 63.333 | 60 | 21 | 1 | 1 | 59 | 119 | 178 | 2.68e-16 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62720.t1 | AT5G15310 | 37.308 | 260 | 90 | 8 | 1 | 239 | 40 | 247 | 1.69e-35 | 126 |
| MS.gene62720.t1 | AT5G15310 | 38.258 | 264 | 82 | 10 | 1 | 239 | 119 | 326 | 6.60e-35 | 127 |
| MS.gene62720.t1 | AT5G15310 | 40.164 | 244 | 94 | 11 | 1 | 239 | 119 | 315 | 2.71e-31 | 117 |
| MS.gene62720.t1 | AT5G15310 | 73.585 | 53 | 12 | 1 | 1 | 53 | 119 | 169 | 1.45e-15 | 73.9 |
Find 34 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAATTATGTGAACGAATTT+TGG | 0.141100 | 6.3:-7271702 | None:intergenic |
| ATTTCTCCACTATTCATAAT+TGG | 0.239670 | 6.3:-7271809 | None:intergenic |
| ATTATGAATAGTGGAGAAAT+TGG | 0.314925 | 6.3:+7271812 | MS.gene62720:CDS |
| ATCATAATCACTTGAAAGCT+TGG | 0.362434 | 6.3:+7271732 | MS.gene62720:CDS |
| TGTCTATGATTGAATTTGTT+GGG | 0.385408 | 6.3:+7271903 | MS.gene62720:CDS |
| TTTCTCATTACCATTATTGT+TGG | 0.395908 | 6.3:-7271849 | None:intergenic |
| GGAGAAAATACCAACAATAA+TGG | 0.401933 | 6.3:+7271839 | MS.gene62720:CDS |
| TCTTAGTCACATGGCTCAAT+GGG | 0.413275 | 6.3:+7271637 | MS.gene62720:CDS |
| AATGGTGGTGATGGTGGAAA+TGG | 0.425976 | 6.3:+7272154 | MS.gene62720:CDS |
| GTGTCTATGATTGAATTTGT+TGG | 0.441639 | 6.3:+7271902 | MS.gene62720:CDS |
| ATCTTAGTCACATGGCTCAA+TGG | 0.445039 | 6.3:+7271636 | MS.gene62720:CDS |
| GTTGCACCAATTATGAATAG+TGG | 0.464401 | 6.3:+7271803 | MS.gene62720:CDS |
| AGTTCAATTGTGAAAGAAGA+AGG | 0.467308 | 6.3:+7271935 | MS.gene62720:CDS |
| ATGAAGATAACAATAACTAC+TGG | 0.468008 | 6.3:+7272194 | MS.gene62720:CDS |
| GGTGATGGTGGAAATGGAAG+TGG | 0.475445 | 6.3:+7272160 | MS.gene62720:CDS |
| ATGATCAAGATCAAGATCAA+TGG | 0.499871 | 6.3:+7271963 | MS.gene62720:CDS |
| TCATGAGTTGACTATGTCAA+TGG | 0.502069 | 6.3:+7272027 | MS.gene62720:CDS |
| CAAAACATTGGTGAATCAGA+TGG | 0.512301 | 6.3:-7272247 | None:intergenic |
| AATAGTGGAGAAATTGGAGG+AGG | 0.518144 | 6.3:+7271818 | MS.gene62720:CDS |
| TTGAAAGATAAAGTTGAAGT+AGG | 0.524078 | 6.3:-7271773 | None:intergenic |
| GACTTGAGTTTGTCAGAGAG+TGG | 0.538409 | 6.3:+7272118 | MS.gene62720:CDS |
| GATGATGTCATTGCTGAAGA+AGG | 0.549060 | 6.3:+7272061 | MS.gene62720:CDS |
| ATTAATAATGATGAGAACAA+TGG | 0.557396 | 6.3:+7271875 | MS.gene62720:CDS |
| ATGATGTCATTGCTGAAGAA+GGG | 0.582083 | 6.3:+7272062 | MS.gene62720:CDS |
| GAATCTAACAATGGTGGTGA+TGG | 0.584861 | 6.3:+7272145 | MS.gene62720:CDS |
| CAAGATCAAGATCAATGGAA+AGG | 0.588024 | 6.3:+7271968 | MS.gene62720:CDS |
| ATGAATAGTGGAGAAATTGG+AGG | 0.588583 | 6.3:+7271815 | MS.gene62720:CDS |
| TCTAACAATGGTGGTGATGG+TGG | 0.594929 | 6.3:+7272148 | MS.gene62720:CDS |
| CACATCTTATATCAAAACAT+TGG | 0.606562 | 6.3:-7272259 | None:intergenic |
| TTGAGTTTGTCAGAGAGTGG+AGG | 0.626434 | 6.3:+7272121 | MS.gene62720:CDS |
| TCAATTGTGAAAGAAGAAGG+TGG | 0.631897 | 6.3:+7271938 | MS.gene62720:CDS |
| TGCTTCAAATCTTAGTCACA+TGG | 0.636039 | 6.3:+7271628 | MS.gene62720:CDS |
| AGTGGAGGTGAATCTAACAA+TGG | 0.688377 | 6.3:+7272136 | MS.gene62720:CDS |
| GGAGGTGAATCTAACAATGG+TGG | 0.762552 | 6.3:+7272139 | MS.gene62720:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAATAATGATGAGAACAA+TGG | + | chr6.3:7271875-7271894 | MS.gene62720:CDS | 20.0% |
| ! | ATGAAGATAACAATAACTAC+TGG | + | chr6.3:7272194-7272213 | MS.gene62720:CDS | 25.0% |
| ! | ATTTCTCCACTATTCATAAT+TGG | - | chr6.3:7271812-7271831 | None:intergenic | 25.0% |
| ! | TTGAAAGATAAAGTTGAAGT+AGG | - | chr6.3:7271776-7271795 | None:intergenic | 25.0% |
| ! | TTTCTCATTACCATTATTGT+TGG | - | chr6.3:7271852-7271871 | None:intergenic | 25.0% |
| !! | ATTATGAATAGTGGAGAAAT+TGG | + | chr6.3:7271812-7271831 | MS.gene62720:CDS | 25.0% |
| !! | TGAATTATGTGAACGAATTT+TGG | - | chr6.3:7271705-7271724 | None:intergenic | 25.0% |
| !! | TGTCTATGATTGAATTTGTT+GGG | + | chr6.3:7271903-7271922 | MS.gene62720:CDS | 25.0% |
| AGTTCAATTGTGAAAGAAGA+AGG | + | chr6.3:7271935-7271954 | MS.gene62720:CDS | 30.0% | |
| ATCATAATCACTTGAAAGCT+TGG | + | chr6.3:7271732-7271751 | MS.gene62720:CDS | 30.0% | |
| ATGATCAAGATCAAGATCAA+TGG | + | chr6.3:7271963-7271982 | MS.gene62720:CDS | 30.0% | |
| GGAGAAAATACCAACAATAA+TGG | + | chr6.3:7271839-7271858 | MS.gene62720:CDS | 30.0% | |
| GTGTCTATGATTGAATTTGT+TGG | + | chr6.3:7271902-7271921 | MS.gene62720:CDS | 30.0% | |
| !! | GAAAGAAGTGCATCATTTTT+AGG | - | chr6.3:7271581-7271600 | None:intergenic | 30.0% |
| !!! | TTTTTAGGTTTGTGTGTAAC+TGG | - | chr6.3:7271566-7271585 | None:intergenic | 30.0% |
| ATGATGTCATTGCTGAAGAA+GGG | + | chr6.3:7272062-7272081 | MS.gene62720:CDS | 35.0% | |
| CAAAACATTGGTGAATCAGA+TGG | - | chr6.3:7272250-7272269 | None:intergenic | 35.0% | |
| CAAGATCAAGATCAATGGAA+AGG | + | chr6.3:7271968-7271987 | MS.gene62720:CDS | 35.0% | |
| GTTGCACCAATTATGAATAG+TGG | + | chr6.3:7271803-7271822 | MS.gene62720:CDS | 35.0% | |
| TCAATTGTGAAAGAAGAAGG+TGG | + | chr6.3:7271938-7271957 | MS.gene62720:CDS | 35.0% | |
| TGCTTCAAATCTTAGTCACA+TGG | + | chr6.3:7271628-7271647 | MS.gene62720:CDS | 35.0% | |
| ! | ATGAATAGTGGAGAAATTGG+AGG | + | chr6.3:7271815-7271834 | MS.gene62720:CDS | 35.0% |
| ! | TCATGAGTTGACTATGTCAA+TGG | + | chr6.3:7272027-7272046 | MS.gene62720:CDS | 35.0% |
| AGTGGAGGTGAATCTAACAA+TGG | + | chr6.3:7272136-7272155 | MS.gene62720:CDS | 40.0% | |
| ATCTTAGTCACATGGCTCAA+TGG | + | chr6.3:7271636-7271655 | MS.gene62720:CDS | 40.0% | |
| GATGATGTCATTGCTGAAGA+AGG | + | chr6.3:7272061-7272080 | MS.gene62720:CDS | 40.0% | |
| TCTTAGTCACATGGCTCAAT+GGG | + | chr6.3:7271637-7271656 | MS.gene62720:CDS | 40.0% | |
| ! | AATAGTGGAGAAATTGGAGG+AGG | + | chr6.3:7271818-7271837 | MS.gene62720:CDS | 40.0% |
| ! | GAATCTAACAATGGTGGTGA+TGG | + | chr6.3:7272145-7272164 | MS.gene62720:CDS | 40.0% |
| GACTTGAGTTTGTCAGAGAG+TGG | + | chr6.3:7272118-7272137 | MS.gene62720:CDS | 45.0% | |
| GGAGGTGAATCTAACAATGG+TGG | + | chr6.3:7272139-7272158 | MS.gene62720:CDS | 45.0% | |
| TTGAGTTTGTCAGAGAGTGG+AGG | + | chr6.3:7272121-7272140 | MS.gene62720:CDS | 45.0% | |
| !! | AATGGTGGTGATGGTGGAAA+TGG | + | chr6.3:7272154-7272173 | MS.gene62720:CDS | 45.0% |
| !! | TCTAACAATGGTGGTGATGG+TGG | + | chr6.3:7272148-7272167 | MS.gene62720:CDS | 45.0% |
| !! | GGTGATGGTGGAAATGGAAG+TGG | + | chr6.3:7272160-7272179 | MS.gene62720:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 7271551 | 7272270 | 7271551 | ID=MS.gene62720 |
| chr6.3 | mRNA | 7271551 | 7272270 | 7271551 | ID=MS.gene62720.t1;Parent=MS.gene62720 |
| chr6.3 | exon | 7271551 | 7272270 | 7271551 | ID=MS.gene62720.t1.exon1;Parent=MS.gene62720.t1 |
| chr6.3 | CDS | 7271551 | 7272270 | 7271551 | ID=cds.MS.gene62720.t1;Parent=MS.gene62720.t1 |
| Gene Sequence |
| Protein sequence |
