Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050103.t1 | XP_013446530.1 | 78.6 | 98 | 21 | 0 | 1 | 98 | 1 | 98 | 1.20E-31 | 145.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050103.t1 | A0A072TUN2 | 78.6 | 98 | 21 | 0 | 1 | 98 | 1 | 98 | 8.8e-32 | 145.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050103 | MS.gene057869 | 0.80897 | 2.38E-50 | -1.69E-46 |
| MS.gene050103 | MS.gene64417 | 0.806758 | 7.04E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050103.t1 | MTR_8g080190 | 78.571 | 98 | 21 | 0 | 1 | 98 | 1 | 98 | 7.83e-32 | 117 |
| MS.gene050103.t1 | MTR_8g080190 | 78.571 | 98 | 21 | 0 | 1 | 98 | 1 | 98 | 7.94e-32 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGTTGCTGTTATTAA+TGG | 0.234139 | 4.4:+16359658 | MS.gene050103:CDS |
| TTACCACGAAAACGAGTTTA+AGG | 0.247286 | 4.4:+16359501 | MS.gene050103:CDS |
| GGGTGAGGCAGAAGGATGTT+AGG | 0.280799 | 4.4:+16359632 | MS.gene050103:CDS |
| GAGATTCCAGAGATAGGATT+AGG | 0.320055 | 4.4:+16359611 | MS.gene050103:CDS |
| TCTTCCAGCCGGAGTGATTC+CGG | 0.368458 | 4.4:+16359694 | MS.gene050103:CDS |
| CTGTTATTAATGGTAATTAT+CGG | 0.374727 | 4.4:+16359668 | MS.gene050103:CDS |
| GTATGATCCGGTTAGGCAAT+GGG | 0.408780 | 4.4:+16359575 | MS.gene050103:CDS |
| GGTGAGGCAGAAGGATGTTA+GGG | 0.410047 | 4.4:+16359633 | MS.gene050103:CDS |
| CCCGCCTTGAAACTTCCAAT+TGG | 0.416167 | 4.4:-16359533 | None:intergenic |
| AGATTCCAGAGATAGGATTA+GGG | 0.432389 | 4.4:+16359612 | MS.gene050103:CDS |
| TCCGGTAGCAGTGGCAGCCT+TGG | 0.443004 | 4.4:+16359712 | MS.gene050103:CDS |
| TTAAGGATCACGAGTCCAAT+TGG | 0.446386 | 4.4:+16359518 | MS.gene050103:CDS |
| AGCTCACCAGGCTCTCTAAT+CGG | 0.449923 | 4.4:-16359767 | None:intergenic |
| CCTCACCCTAATCCTATCTC+TGG | 0.463374 | 4.4:-16359617 | None:intergenic |
| GGGGCAGAGATTCCAGAGAT+AGG | 0.464958 | 4.4:+16359605 | MS.gene050103:CDS |
| ATAGATAGATTTATGGCTGC+TGG | 0.474893 | 4.4:+16359466 | None:intergenic |
| ACCAAGGCTGCCACTGCTAC+CGG | 0.481499 | 4.4:-16359713 | None:intergenic |
| TAGGATTAGGGTGAGGCAGA+AGG | 0.482821 | 4.4:+16359624 | MS.gene050103:CDS |
| GAGTCCAATTGGAAGTTTCA+AGG | 0.487269 | 4.4:+16359529 | MS.gene050103:CDS |
| CGAACGAGTATGATCCGGTT+AGG | 0.499273 | 4.4:+16359568 | MS.gene050103:CDS |
| CCAATTGGAAGTTTCAAGGC+GGG | 0.502023 | 4.4:+16359533 | MS.gene050103:CDS |
| GTGGCAGCCTTGGTCCTCGC+CGG | 0.511363 | 4.4:+16359722 | MS.gene050103:CDS |
| AGTATGATCCGGTTAGGCAA+TGG | 0.514232 | 4.4:+16359574 | MS.gene050103:CDS |
| TTTATGGCTGCTGGCAGACA+TGG | 0.522722 | 4.4:+16359475 | None:intergenic |
| AAATTCGAACGAGTATGATC+CGG | 0.536782 | 4.4:+16359563 | MS.gene050103:CDS |
| GCTACCGGAATCACTCCGGC+TGG | 0.551956 | 4.4:-16359698 | None:intergenic |
| AACCACACCGGCGAGGACCA+AGG | 0.564324 | 4.4:-16359729 | None:intergenic |
| CAATTGGAAGTTTCAAGGCG+GGG | 0.579096 | 4.4:+16359534 | MS.gene050103:CDS |
| ATGATCCGGTTAGGCAATGG+GGG | 0.580356 | 4.4:+16359577 | MS.gene050103:CDS |
| TATGATCCGGTTAGGCAATG+GGG | 0.583171 | 4.4:+16359576 | MS.gene050103:CDS |
| CGGAGTGATTCCGGTAGCAG+TGG | 0.587437 | 4.4:+16359703 | MS.gene050103:CDS |
| AGCCTTGGTCCTCGCCGGTG+TGG | 0.605000 | 4.4:+16359727 | MS.gene050103:CDS |
| ATTATCGGTCATCTTCCAGC+CGG | 0.610184 | 4.4:+16359683 | MS.gene050103:CDS |
| CACTGCTACCGGAATCACTC+CGG | 0.615938 | 4.4:-16359702 | None:intergenic |
| CCAGAGATAGGATTAGGGTG+AGG | 0.627823 | 4.4:+16359617 | MS.gene050103:CDS |
| TGATCCGGTTAGGCAATGGG+GGG | 0.662940 | 4.4:+16359578 | MS.gene050103:CDS |
| TCCAATTGGAAGTTTCAAGG+CGG | 0.677428 | 4.4:+16359532 | MS.gene050103:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTGTTATTAATGGTAATTAT+CGG | + | chr4.4:16359668-16359687 | MS.gene050103:CDS | 20.0% |
| !! | AAAGAAGTTGCTGTTATTAA+TGG | + | chr4.4:16359658-16359677 | MS.gene050103:CDS | 25.0% |
| AAATTCGAACGAGTATGATC+CGG | + | chr4.4:16359563-16359582 | MS.gene050103:CDS | 35.0% | |
| AGATTCCAGAGATAGGATTA+GGG | + | chr4.4:16359612-16359631 | MS.gene050103:CDS | 35.0% | |
| TTACCACGAAAACGAGTTTA+AGG | + | chr4.4:16359501-16359520 | MS.gene050103:CDS | 35.0% | |
| GAGATTCCAGAGATAGGATT+AGG | + | chr4.4:16359611-16359630 | MS.gene050103:CDS | 40.0% | |
| GAGTCCAATTGGAAGTTTCA+AGG | + | chr4.4:16359529-16359548 | MS.gene050103:CDS | 40.0% | |
| TCCAATTGGAAGTTTCAAGG+CGG | + | chr4.4:16359532-16359551 | MS.gene050103:CDS | 40.0% | |
| TTAAGGATCACGAGTCCAAT+TGG | + | chr4.4:16359518-16359537 | MS.gene050103:CDS | 40.0% | |
| ! | GATCCTTAAACTCGTTTTCG+TGG | - | chr4.4:16359507-16359526 | None:intergenic | 40.0% |
| ACTTCGCAGAAAAACCACAC+CGG | - | chr4.4:16359744-16359763 | None:intergenic | 45.0% | |
| AGTATGATCCGGTTAGGCAA+TGG | + | chr4.4:16359574-16359593 | MS.gene050103:CDS | 45.0% | |
| ATTATCGGTCATCTTCCAGC+CGG | + | chr4.4:16359683-16359702 | MS.gene050103:CDS | 45.0% | |
| CAATTGGAAGTTTCAAGGCG+GGG | + | chr4.4:16359534-16359553 | MS.gene050103:CDS | 45.0% | |
| CCAATTGGAAGTTTCAAGGC+GGG | + | chr4.4:16359533-16359552 | MS.gene050103:CDS | 45.0% | |
| GTATGATCCGGTTAGGCAAT+GGG | + | chr4.4:16359575-16359594 | MS.gene050103:CDS | 45.0% | |
| TATGATCCGGTTAGGCAATG+GGG | + | chr4.4:16359576-16359595 | MS.gene050103:CDS | 45.0% | |
| ATGATCCGGTTAGGCAATGG+GGG | + | chr4.4:16359577-16359596 | MS.gene050103:CDS | 50.0% | |
| CCAGAGATAGGATTAGGGTG+AGG | + | chr4.4:16359617-16359636 | MS.gene050103:CDS | 50.0% | |
| CCCGCCTTGAAACTTCCAAT+TGG | - | chr4.4:16359536-16359555 | None:intergenic | 50.0% | |
| CCTCACCCTAATCCTATCTC+TGG | - | chr4.4:16359620-16359639 | None:intergenic | 50.0% | |
| CGAACGAGTATGATCCGGTT+AGG | + | chr4.4:16359568-16359587 | MS.gene050103:CDS | 50.0% | |
| GGTGAGGCAGAAGGATGTTA+GGG | + | chr4.4:16359633-16359652 | MS.gene050103:CDS | 50.0% | |
| TAGGATTAGGGTGAGGCAGA+AGG | + | chr4.4:16359624-16359643 | MS.gene050103:CDS | 50.0% | |
| CACTGCTACCGGAATCACTC+CGG | - | chr4.4:16359705-16359724 | None:intergenic | 55.0% | |
| GGGGCAGAGATTCCAGAGAT+AGG | + | chr4.4:16359605-16359624 | MS.gene050103:CDS | 55.0% | |
| GGGTGAGGCAGAAGGATGTT+AGG | + | chr4.4:16359632-16359651 | MS.gene050103:CDS | 55.0% | |
| TCTTCCAGCCGGAGTGATTC+CGG | + | chr4.4:16359694-16359713 | MS.gene050103:CDS | 55.0% | |
| TGATCCGGTTAGGCAATGGG+GGG | + | chr4.4:16359578-16359597 | MS.gene050103:CDS | 55.0% | |
| ACCAAGGCTGCCACTGCTAC+CGG | - | chr4.4:16359716-16359735 | None:intergenic | 60.0% | |
| CACTCCCCCCATTGCCTAAC+CGG | - | chr4.4:16359585-16359604 | None:intergenic | 60.0% | |
| CGGAGTGATTCCGGTAGCAG+TGG | + | chr4.4:16359703-16359722 | MS.gene050103:CDS | 60.0% | |
| GCAGAAAAACCACACCGGCG+AGG | - | chr4.4:16359739-16359758 | None:intergenic | 60.0% | |
| GGTTAGGCAATGGGGGGAGT+GGG | + | chr4.4:16359584-16359603 | MS.gene050103:CDS | 60.0% | |
| GTTAGGCAATGGGGGGAGTG+GGG | + | chr4.4:16359585-16359604 | MS.gene050103:CDS | 60.0% | |
| TTAGGCAATGGGGGGAGTGG+GGG | + | chr4.4:16359586-16359605 | MS.gene050103:CDS | 60.0% | |
| AACCACACCGGCGAGGACCA+AGG | - | chr4.4:16359732-16359751 | None:intergenic | 65.0% | |
| CGGTTAGGCAATGGGGGGAG+TGG | + | chr4.4:16359583-16359602 | MS.gene050103:CDS | 65.0% | |
| GCTACCGGAATCACTCCGGC+TGG | - | chr4.4:16359701-16359720 | None:intergenic | 65.0% | |
| TCCGGTAGCAGTGGCAGCCT+TGG | + | chr4.4:16359712-16359731 | MS.gene050103:CDS | 65.0% | |
| AGCCTTGGTCCTCGCCGGTG+TGG | + | chr4.4:16359727-16359746 | MS.gene050103:CDS | 70.0% | |
| GTGGCAGCCTTGGTCCTCGC+CGG | + | chr4.4:16359722-16359741 | MS.gene050103:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 16359478 | 16359774 | 16359478 | ID=MS.gene050103 |
| chr4.4 | mRNA | 16359478 | 16359774 | 16359478 | ID=MS.gene050103.t1;Parent=MS.gene050103 |
| chr4.4 | exon | 16359478 | 16359774 | 16359478 | ID=MS.gene050103.t1.exon1;Parent=MS.gene050103.t1 |
| chr4.4 | CDS | 16359478 | 16359774 | 16359478 | ID=cds.MS.gene050103.t1;Parent=MS.gene050103.t1 |
| Gene Sequence |
| Protein sequence |
