AlfalfaGEDB | MS.gene050219

M. sativa cultivar XinJiangDaYe / MS.gene050219

KEGG: K14431
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006139 GO:0006351 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0018130 GO:0019438 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0046483 GO:0071704 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576
PFAM: PF14144.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050219.t1	XP_003591665.2	93.3	149	9	1	1	149	93	240	2.20E-72	281.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050219.t1	Q39162	40.6	165	80	5	1	148	191	354	6.7e-22	105.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050219.t1	I3S1H9	93.3	149	9	1	1	149	93	240	1.6e-72	281.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050218	MS.gene050219	0.992809	1.41E-195	-1.69E-46
MS.gene050219	MS.gene050221	0.855014	8.38E-62	-1.69E-46
MS.gene050219	MS.gene21599	0.897082	1.98E-76	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050219.t1	MTR_1g090370	93.289	149	9	1	1	149	81	228	3.81e-101	289
MS.gene050219.t1	MTR_3g087940	40.120	167	80	4	1	149	190	354	1.33e-28	108
MS.gene050219.t1	MTR_8g091650	37.725	167	84	4	1	149	112	276	1.47e-26	101
MS.gene050219.t1	MTR_8g091650	37.725	167	84	4	1	149	185	349	5.72e-26	100
MS.gene050219.t1	MTR_8g091650	37.725	167	84	4	1	149	221	385	8.48e-26	100
MS.gene050219.t1	MTR_7g089800	37.931	145	73	3	21	149	273	416	7.01e-20	84.7
MS.gene050219.t1	MTR_7g089800	37.931	145	73	3	21	149	302	445	8.48e-20	84.7
MS.gene050219.t1	MTR_7g092750	34.965	143	77	3	21	149	215	355	1.85e-19	83.2
MS.gene050219.t1	MTR_7g092750	34.965	143	77	3	21	149	316	456	3.19e-19	83.2
MS.gene050219.t1	MTR_8g040120	34.682	173	88	4	1	149	288	459	1.39e-18	81.3
MS.gene050219.t1	MTR_2g086420	39.706	136	70	5	23	149	329	461	2.00e-18	80.9
MS.gene050219.t1	MTR_2g086420	39.706	136	70	5	23	149	330	462	2.02e-18	80.9
MS.gene050219.t1	MTR_1g111310	34.320	169	90	3	1	149	158	325	2.62e-17	77.8
MS.gene050219.t1	MTR_1g111310	34.320	169	90	3	1	149	533	700	2.62e-17	77.8
MS.gene050219.t1	MTR_3g073560	33.129	163	81	4	1	141	176	332	2.57e-16	75.1
MS.gene050219.t1	MTR_5g060950	36.232	138	78	3	21	149	206	342	4.80e-16	73.9
MS.gene050219.t1	MTR_4g053250	36.496	137	79	3	20	149	319	454	6.01e-16	73.9
MS.gene050219.t1	MTR_0126s0080	45.070	71	37	2	21	91	359	427	6.02e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050219.t1	AT5G10030	40.606	165	80	5	1	148	191	354	1.20e-27	105
MS.gene050219.t1	AT5G10030	40.606	165	80	5	1	148	191	354	1.20e-27	105
MS.gene050219.t1	AT5G10030	40.606	165	80	5	1	148	191	354	1.20e-27	105
MS.gene050219.t1	AT5G10030	40.606	165	80	5	1	148	191	354	1.20e-27	105
MS.gene050219.t1	AT5G10030	40.606	165	80	5	1	148	191	354	1.20e-27	105
MS.gene050219.t1	AT5G65210	39.759	166	80	4	1	148	125	288	2.85e-26	100
MS.gene050219.t1	AT5G65210	39.873	158	75	4	1	140	195	350	1.11e-25	100
MS.gene050219.t1	AT5G65210	39.873	158	75	4	1	140	195	350	1.11e-25	100
MS.gene050219.t1	AT5G65210	39.873	158	75	4	1	140	195	350	1.11e-25	100
MS.gene050219.t1	AT5G65210	39.873	158	75	4	1	140	195	350	1.11e-25	100
MS.gene050219.t1	AT5G65210	39.873	158	75	4	1	140	195	350	1.11e-25	100
MS.gene050219.t1	AT5G65210	39.873	158	75	4	1	140	195	350	1.11e-25	100
MS.gene050219.t1	AT1G77920	36.310	168	86	4	1	149	194	359	2.88e-24	96.7
MS.gene050219.t1	AT1G22070	34.524	168	90	3	1	149	209	375	5.77e-21	87.8
MS.gene050219.t1	AT1G08320	36.145	166	86	7	1	149	160	322	2.44e-19	83.2
MS.gene050219.t1	AT1G08320	36.145	166	86	7	1	149	160	322	2.44e-19	83.2
MS.gene050219.t1	AT1G08320	36.145	166	86	7	1	149	284	446	8.54e-19	82.0
MS.gene050219.t1	AT1G08320	36.145	166	86	7	1	149	284	446	8.54e-19	82.0
MS.gene050219.t1	AT1G08320	36.145	166	86	7	1	149	284	446	8.54e-19	82.0
MS.gene050219.t1	AT5G06950	34.302	172	89	3	1	149	153	323	9.37e-19	81.3
MS.gene050219.t1	AT5G06950	34.302	172	89	3	1	149	153	323	9.37e-19	81.3
MS.gene050219.t1	AT5G06950	34.302	172	89	3	1	149	153	323	9.37e-19	81.3
MS.gene050219.t1	AT5G06950	34.302	172	89	3	1	149	153	323	9.37e-19	81.3
MS.gene050219.t1	AT5G06950	34.302	172	89	3	1	149	153	323	9.37e-19	81.3
MS.gene050219.t1	AT5G06960	35.465	172	87	3	1	149	133	303	3.29e-18	79.3
MS.gene050219.t1	AT5G06960	35.465	172	87	3	1	149	153	323	4.20e-18	79.3
MS.gene050219.t1	AT5G06960	35.465	172	87	3	1	149	153	323	4.20e-18	79.3
MS.gene050219.t1	AT5G06839	32.000	175	92	3	1	149	236	409	5.40e-18	79.7
MS.gene050219.t1	AT5G06839	32.000	175	92	3	1	149	235	408	5.43e-18	79.7
MS.gene050219.t1	AT5G06839	32.000	175	92	3	1	149	278	451	7.33e-18	79.3
MS.gene050219.t1	AT3G12250	33.140	172	91	3	1	149	126	296	4.97e-17	76.3
MS.gene050219.t1	AT3G12250	33.140	172	91	3	1	149	147	317	6.45e-17	75.9
MS.gene050219.t1	AT3G12250	33.140	172	91	3	1	149	178	348	8.02e-17	75.9
MS.gene050219.t1	AT3G12250	33.140	172	91	4	1	149	153	323	8.43e-17	75.9
MS.gene050219.t1	AT3G12250	33.140	172	91	4	1	149	153	323	8.43e-17	75.9
MS.gene050219.t1	AT3G12250	33.140	172	91	4	1	149	153	323	8.43e-17	75.9
MS.gene050219.t1	AT3G12250	33.140	172	91	4	1	149	153	323	8.43e-17	75.9
MS.gene050219.t1	AT1G68640	33.140	172	91	4	1	149	275	445	1.15e-12	64.7

Find 22 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTATGATATTTATAATCT+TGG	0.237785	1.2:+67646963	MS.gene050219:CDS
TCGTCCTTCTCAACTTCTTC+AGG	0.297180	1.2:+67646684	MS.gene050219:CDS
CAACATGTGAACGCAATCTA+TGG	0.375038	1.2:+67646647	MS.gene050219:CDS
TTGCTTTATCAATGATTTGT+TGG	0.412400	1.2:-67647037	None:intergenic
TTACCTCTTTGAAGAAGCTT+AGG	0.413182	1.2:-67647106	None:intergenic
AAAATTGATGCAAGAATTGC+CGG	0.420339	1.2:-67647255	None:intergenic
ATTGATAAAGCAACATTAGC+AGG	0.485264	1.2:+67647047	MS.gene050219:CDS
CAAAGTGAGCTCCGAGGCTG+CGG	0.496681	1.2:-67647343	None:intergenic
GAGGCTGCGGATTATGTAGA+TGG	0.504063	1.2:-67647330	None:intergenic
TTGAAGAAGCTTAGGTGTTG+AGG	0.516308	1.2:-67647098	None:intergenic
CGCAATCTATGGTGGATTGG+TGG	0.541391	1.2:+67646658	MS.gene050219:CDS
ATCTACATAATCCGCAGCCT+CGG	0.542523	1.2:+67647332	MS.gene050219:CDS
TAGATTGCGTTCACATGTTG+TGG	0.545132	1.2:-67646644	None:intergenic
TACAGGCTGATAATCTTCGC+CGG	0.591845	1.2:+67647236	MS.gene050219:intron
GTGCACAAATGTTTCAGTTG+AGG	0.592785	1.2:-67646930	None:intergenic
ACACCTAAGCTTCTTCAAAG+AGG	0.598796	1.2:+67647103	MS.gene050219:CDS
CATGTGAACGCAATCTATGG+TGG	0.599889	1.2:+67646650	MS.gene050219:CDS
GAACGCAATCTATGGTGGAT+TGG	0.599907	1.2:+67646655	MS.gene050219:CDS
GCTGAATATGCTCTTGCACA+AGG	0.606518	1.2:+67647002	MS.gene050219:CDS
TGAGCTCGTAGTTACTCTGA+AGG	0.607552	1.2:+67647306	MS.gene050219:CDS
ATTACCTGAAGAAGTTGAGA+AGG	0.659326	1.2:-67646688	None:intergenic
AATTCTCAAAGTGAGCTCCG+AGG	0.740720	1.2:-67647349	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTTATGATATTTATAATCT+TGG	+	chr1.2:67646963-67646982	MS.gene050219:CDS	15.0%
!!	TAAGAAAACATAATTCAAAT+TGG	-	chr1.2:67646867-67646886	None:intergenic	15.0%
!!	AAGTACTATGTGATTTAATA+TGG	+	chr1.2:67646888-67646907	MS.gene050219:intron	20.0%
!!	GATTTAATATGGATATACAT+AGG	+	chr1.2:67646899-67646918	MS.gene050219:intron	20.0%
!!	TAGGGTTAAAACTTAAAAAT+AGG	-	chr1.2:67647134-67647153	None:intergenic	20.0%
!!	TTGCTTTATCAATGATTTGT+TGG	-	chr1.2:67647040-67647059	None:intergenic	25.0%
	AAAATTGATGCAAGAATTGC+CGG	-	chr1.2:67647258-67647277	None:intergenic	30.0%
	AACTAATTCTACCCCTAATT+AGG	-	chr1.2:67646829-67646848	None:intergenic	30.0%
	ATTGATAAAGCAACATTAGC+AGG	+	chr1.2:67647047-67647066	MS.gene050219:CDS	30.0%
	ATTGATGTGATGTCCTAATT+AGG	+	chr1.2:67646813-67646832	MS.gene050219:intron	30.0%
	CATCATATAGAAAACTGACT+TGG	+	chr1.2:67647202-67647221	MS.gene050219:intron	30.0%
	TTGATGTGATGTCCTAATTA+GGG	+	chr1.2:67646814-67646833	MS.gene050219:intron	30.0%
!!!	GAAGCACTGTTTTGAATTAT+TGG	+	chr1.2:67646588-67646607	MS.gene050219:CDS	30.0%
	ACTTGGTTTCTTGTAACTAC+AGG	+	chr1.2:67647219-67647238	MS.gene050219:intron	35.0%
	ATTACCTGAAGAAGTTGAGA+AGG	-	chr1.2:67646691-67646710	None:intergenic	35.0%
	TGATGTGATGTCCTAATTAG+GGG	+	chr1.2:67646815-67646834	MS.gene050219:intron	35.0%
!	TTACCTCTTTGAAGAAGCTT+AGG	-	chr1.2:67647109-67647128	None:intergenic	35.0%
	ACACCTAAGCTTCTTCAAAG+AGG	+	chr1.2:67647103-67647122	MS.gene050219:CDS	40.0%
	CAACATGTGAACGCAATCTA+TGG	+	chr1.2:67646647-67646666	MS.gene050219:CDS	40.0%
	GTGCACAAATGTTTCAGTTG+AGG	-	chr1.2:67646933-67646952	None:intergenic	40.0%
	TAGATTGCGTTCACATGTTG+TGG	-	chr1.2:67646647-67646666	None:intergenic	40.0%
!	TTGAAGAAGCTTAGGTGTTG+AGG	-	chr1.2:67647101-67647120	None:intergenic	40.0%
	ACCCTACACACTCTCTAACA+CGG	+	chr1.2:67647148-67647167	MS.gene050219:intron	45.0%
	ATCTACATAATCCGCAGCCT+CGG	+	chr1.2:67647332-67647351	MS.gene050219:CDS	45.0%
	CATGTGAACGCAATCTATGG+TGG	+	chr1.2:67646650-67646669	MS.gene050219:CDS	45.0%
	GCTGAATATGCTCTTGCACA+AGG	+	chr1.2:67647002-67647021	MS.gene050219:CDS	45.0%
	TACAGGCTGATAATCTTCGC+CGG	+	chr1.2:67647236-67647255	MS.gene050219:intron	45.0%
	TCGTCCTTCTCAACTTCTTC+AGG	+	chr1.2:67646684-67646703	MS.gene050219:CDS	45.0%
	TGAGCTCGTAGTTACTCTGA+AGG	+	chr1.2:67647306-67647325	MS.gene050219:CDS	45.0%
!!	GAACGCAATCTATGGTGGAT+TGG	+	chr1.2:67646655-67646674	MS.gene050219:CDS	45.0%
!	AGCCGTGTTAGAGAGTGTGT+AGG	-	chr1.2:67647153-67647172	None:intergenic	50.0%
!	GAGGCTGCGGATTATGTAGA+TGG	-	chr1.2:67647333-67647352	None:intergenic	50.0%
!	GCCGTGTTAGAGAGTGTGTA+GGG	-	chr1.2:67647152-67647171	None:intergenic	50.0%
!!	CGCAATCTATGGTGGATTGG+TGG	+	chr1.2:67646658-67646677	MS.gene050219:CDS	50.0%
	CAAAGTGAGCTCCGAGGCTG+CGG	-	chr1.2:67647346-67647365	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	67646586	67647366	67646586	ID=MS.gene050219
chr1.2	mRNA	67646586	67647366	67646586	ID=MS.gene050219.t1;Parent=MS.gene050219
chr1.2	exon	67646586	67646705	67646586	ID=MS.gene050219.t1.exon1;Parent=MS.gene050219.t1
chr1.2	CDS	67646586	67646705	67646586	ID=cds.MS.gene050219.t1;Parent=MS.gene050219.t1
chr1.2	exon	67646921	67647124	67646921	ID=MS.gene050219.t1.exon2;Parent=MS.gene050219.t1
chr1.2	CDS	67646921	67647124	67646921	ID=cds.MS.gene050219.t1;Parent=MS.gene050219.t1
chr1.2	exon	67647241	67647366	67647241	ID=MS.gene050219.t1.exon3;Parent=MS.gene050219.t1
chr1.2	CDS	67647241	67647366	67647241	ID=cds.MS.gene050219.t1;Parent=MS.gene050219.t1

Gene Sequence

>MS.gene050219

ATGAAGCACTGTTTTGAATTATTGGAGATAAAAACACGTTCTGCAAATGTAGATTCTGCCACAACATGTGAACGCAATCTATGGTGGATTGGTGGCTTTCGTCCTTCTCAACTTCTTCAGGTAATGCATGTTGATATTTTGTAAATATGAGTCAGACTCAATTTCAACACTATATATTGTCACTTACGCATTCATTTTGTTCTTTATATTTTTTTAGTGCTAAAAAGATTGATGTGATGTCCTAATTAGGGGTAGAATTAGTTAGAAACATATACACACCAATTTGAATTATGTTTTCTTACAAGTACTATGTGATTTAATATGGATATACATAGGTTATTCTGCCTCAACTGAAACATTTGTGCACTCAACAACAACTTTATGATATTTATAATCTTGGACAATCATGTCAACAAGCTGAATATGCTCTTGCACAAGGCATGATAGAACTCCAACAAATCATTGATAAAGCAACATTAGCAGGTGACAAAGAGTTTCAGCAGACGTGTGTACCTCAACACCTAAGCTTCTTCAAAGAGGTAATACCTATTTTTAAGTTTTAACCCTACACACTCTCTAACACGGCTCGATTATAAGTTGAAATTCATGCGAGTCTCATCATATAGAAAACTGACTTGGTTTCTTGTAACTACAGGCTGATAATCTTCGCCGGCAATTCTTGCATCAATTTTCTCGTCTCTTTACAATTTCTCAGCAAGCTGAGCTCGTAGTTACTCTGAAGGACCATCTACATAATCCGCAGCCTCGGAGCTCACTTTGA

Protein sequence

>MS.gene050219.t1

MKHCFELLEIKTRSANVDSATTCERNLWWIGGFRPSQLLQVILPQLKHLCTQQQLYDIYNLGQSCQQAEYALAQGMIELQQIIDKATLAGDKEFQQTCVPQHLSFFKEADNLRRQFLHQFSRLFTISQQAELVVTLKDHLHNPQPRSSL