Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050285.t1 | XP_013450122.1 | 96.1 | 204 | 6 | 2 | 1 | 202 | 54 | 257 | 1.10E-106 | 396 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050285.t1 | Q9FMR3 | 44.4 | 196 | 93 | 3 | 1 | 196 | 55 | 234 | 4.8e-39 | 162.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050285.t1 | A0A072UEA7 | 96.1 | 204 | 6 | 2 | 1 | 202 | 54 | 257 | 7.7e-107 | 396.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050282 | MS.gene050285 | 0.917929 | 2.92E-86 | -1.69E-46 |
| MS.gene050283 | MS.gene050285 | 0.910704 | 1.39E-82 | -1.69E-46 |
| MS.gene050285 | MS.gene050286 | 0.906334 | 1.66E-80 | -1.69E-46 |
| MS.gene050285 | MS.gene051751 | 0.945244 | 4.38E-104 | -1.69E-46 |
| MS.gene050285 | MS.gene24711 | 0.94222 | 1.06E-101 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCGCAAGTTGAATCTTTC+TGG | 0.167753 | 7.3:+9498709 | None:intergenic |
| GAGCAAGTGGATTGGTTCTT+AGG | 0.188696 | 7.3:-9498546 | MS.gene050285:CDS |
| CTCACTTCACTTTCTTGCCT+TGG | 0.279048 | 7.3:+9499527 | None:intergenic |
| AAGAGGATGTTTCATCTAAC+TGG | 0.287039 | 7.3:+9499327 | None:intergenic |
| TCTTCAGATAATCGCATCAT+TGG | 0.352302 | 7.3:-9499434 | MS.gene050285:CDS |
| TTGGAAAATGAACGAGTATA+AGG | 0.364384 | 7.3:-9499354 | MS.gene050285:CDS |
| TCTGAAGAATAAACATGATT+AGG | 0.369085 | 7.3:+9499449 | None:intergenic |
| TTATGATGCTTTGTTGGATT+GGG | 0.384703 | 7.3:-9498568 | MS.gene050285:CDS |
| ATTATGATGCTTTGTTGGAT+TGG | 0.386486 | 7.3:-9498569 | MS.gene050285:CDS |
| TGCTGATTATGATGCTTTGT+TGG | 0.395773 | 7.3:-9498574 | MS.gene050285:CDS |
| CATTGGAGTGAAAAGAACTA+TGG | 0.407082 | 7.3:-9499417 | MS.gene050285:CDS |
| CCAGTGTTGGTGAGACGCTT+TGG | 0.428965 | 7.3:+9499494 | None:intergenic |
| CATAATCAGCAATATCCATT+TGG | 0.446503 | 7.3:+9498586 | None:intergenic |
| AGCAAGTGGATTGGTTCTTA+GGG | 0.460177 | 7.3:-9498545 | MS.gene050285:CDS |
| TCTATAGAGGAAGAGCTCTC+TGG | 0.482763 | 7.3:+9498645 | None:intergenic |
| CAAATGGAAAGAAAACTGAT+TGG | 0.496748 | 7.3:-9499373 | MS.gene050285:CDS |
| CCAAAGCGTCTCACCAACAC+TGG | 0.504150 | 7.3:-9499494 | MS.gene050285:CDS |
| ACATGGGTTGTAACCCTCCT+TGG | 0.505741 | 7.3:+9498750 | None:intergenic |
| AGCTTCTCCAACCTCTTGTG+AGG | 0.507371 | 7.3:+9498611 | None:intergenic |
| GTCTCACCAACACTGGGTAC+TGG | 0.511931 | 7.3:-9499487 | MS.gene050285:CDS |
| TTCAAACAAAGTAGCTCCTA+AGG | 0.519978 | 7.3:-9499306 | MS.gene050285:intron |
| GGCTTTCCAGTACCCAGTGT+TGG | 0.521942 | 7.3:+9499481 | None:intergenic |
| AGGAGCTACTTTGTTTGAAG+AGG | 0.523871 | 7.3:+9499310 | None:intergenic |
| GGTTGGAGAAGCTAGCCAAA+TGG | 0.532147 | 7.3:-9498601 | MS.gene050285:CDS |
| TATAGTGGAAGAGCTCCAAA+TGG | 0.542069 | 7.3:-9499389 | MS.gene050285:CDS |
| GCTTCTCCAACCTCTTGTGA+GGG | 0.545254 | 7.3:+9498612 | None:intergenic |
| TGGATTGGGAGCAAGTGGAT+TGG | 0.559258 | 7.3:-9498554 | MS.gene050285:CDS |
| GGGTTGAACATGGTCTATAG+AGG | 0.566952 | 7.3:+9498632 | None:intergenic |
| AGTGGATTGGTTCTTAGGGT+CGG | 0.569927 | 7.3:-9498541 | MS.gene050285:CDS |
| GTTCAACCCTCACAAGAGGT+TGG | 0.570806 | 7.3:-9498618 | MS.gene050285:CDS |
| ACTGGGTACTGGAAAGCCAC+TGG | 0.577981 | 7.3:-9499476 | MS.gene050285:CDS |
| CCTCTTGTGAGGGTTGAACA+TGG | 0.579803 | 7.3:+9498622 | None:intergenic |
| ACCCATGTTCAAAATGCTCA+AGG | 0.588355 | 7.3:-9498735 | MS.gene050285:CDS |
| TGGGTTGTAACCCTCCTTGG+TGG | 0.590628 | 7.3:+9498753 | None:intergenic |
| GTTGTAACCCTCCTTGGTGG+TGG | 0.601972 | 7.3:+9498756 | None:intergenic |
| CCATGTTCAACCCTCACAAG+AGG | 0.611064 | 7.3:-9498622 | MS.gene050285:CDS |
| CAAGAAAGTGAAGTGAGAGG+AGG | 0.612857 | 7.3:-9499521 | MS.gene050285:CDS |
| TAGACGACCACCACCAAGGA+GGG | 0.617242 | 7.3:-9498763 | MS.gene050285:CDS |
| ACATGATGTTCAAATCTCGA+TGG | 0.619431 | 7.3:-9498688 | MS.gene050285:CDS |
| CAAAGCGTCTCACCAACACT+GGG | 0.625021 | 7.3:-9499493 | MS.gene050285:CDS |
| GGATGTTTCATCTAACTGGA+TGG | 0.629602 | 7.3:+9499331 | None:intergenic |
| TTTGTTGGATTGGGAGCAAG+TGG | 0.640642 | 7.3:-9498559 | MS.gene050285:CDS |
| CCCATGTTCAAAATGCTCAA+GGG | 0.648110 | 7.3:-9498734 | MS.gene050285:CDS |
| AACATGATTAGGAGATCCAG+TGG | 0.656277 | 7.3:+9499460 | None:intergenic |
| GTATGAGAGACACGGAACAA+TGG | 0.656880 | 7.3:-9499562 | MS.gene050285:CDS |
| AGAGGCAAGTATGAGAGACA+CGG | 0.662506 | 7.3:-9499570 | MS.gene050285:CDS |
| AGGCAAGAAAGTGAAGTGAG+AGG | 0.663868 | 7.3:-9499524 | MS.gene050285:CDS |
| TTGATAGACGACCACCACCA+AGG | 0.683488 | 7.3:-9498767 | MS.gene050285:CDS |
| ATAGACGACCACCACCAAGG+AGG | 0.747279 | 7.3:-9498764 | MS.gene050285:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTAGTTAATTTGTTTTA+CGG | - | chr7.3:9499024-9499043 | MS.gene050285:intron | 10.0% |
| !!! | TTTGATATTGATTATTTAAT+TGG | - | chr7.3:9499259-9499278 | MS.gene050285:intron | 10.0% |
| !!! | ATTTAGTTAATTTGTTTTAC+GGG | - | chr7.3:9499025-9499044 | MS.gene050285:intron | 15.0% |
| !! | AATTCAAACCAATATAAATC+AGG | + | chr7.3:9498907-9498926 | None:intergenic | 20.0% |
| !!! | ATTAACCTTTTGATTAATAC+TGG | - | chr7.3:9499223-9499242 | MS.gene050285:intron | 20.0% |
| !!! | CTAACAGACTTTATTTTTTT+AGG | + | chr7.3:9498864-9498883 | None:intergenic | 20.0% |
| !!! | CTATCTTTATTCTTTTTTTG+AGG | - | chr7.3:9499093-9499112 | MS.gene050285:intron | 20.0% |
| !!! | TTTAGTTAATTTGTTTTACG+GGG | - | chr7.3:9499026-9499045 | MS.gene050285:intron | 20.0% |
| !!! | TTTGTTTTGTTTTGTTTTAC+AGG | - | chr7.3:9499177-9499196 | MS.gene050285:intron | 20.0% |
| ! | ATTAGCCAGTATTAATCAAA+AGG | + | chr7.3:9499231-9499250 | None:intergenic | 25.0% |
| ! | GTATACAACCTGATTTATAT+TGG | - | chr7.3:9498896-9498915 | MS.gene050285:intron | 25.0% |
| ! | TATTTAATTGGTTGCAGTTA+AGG | - | chr7.3:9499271-9499290 | MS.gene050285:intron | 25.0% |
| ! | TCTGAAGAATAAACATGATT+AGG | + | chr7.3:9498667-9498686 | None:intergenic | 25.0% |
| !! | ACAAAAAATCAAAGTGCTTA+AGG | - | chr7.3:9499319-9499338 | MS.gene050285:CDS | 25.0% |
| !! | CAAAAAATCAAAGTGCTTAA+GGG | - | chr7.3:9499320-9499339 | MS.gene050285:CDS | 25.0% |
| !!! | ACTTTGATTTTTTGTACACT+CGG | + | chr7.3:9499314-9499333 | None:intergenic | 25.0% |
| !!! | TTTTTGAGGTTCATATTAGA+TGG | - | chr7.3:9499107-9499126 | MS.gene050285:intron | 25.0% |
| ACCAATATGTACGAAATTCA+AGG | + | chr7.3:9498946-9498965 | None:intergenic | 30.0% | |
| ACCTTGAATTTCGTACATAT+TGG | - | chr7.3:9498942-9498961 | MS.gene050285:intron | 30.0% | |
| ATATGATATGTACGTACCTT+AGG | + | chr7.3:9498826-9498845 | None:intergenic | 30.0% | |
| CAAATGGAAAGAAAACTGAT+TGG | - | chr7.3:9498740-9498759 | MS.gene050285:CDS | 30.0% | |
| CATAATCAGCAATATCCATT+TGG | + | chr7.3:9499530-9499549 | None:intergenic | 30.0% | |
| TATCATATGCATCTTGAACT+AGG | - | chr7.3:9498838-9498857 | MS.gene050285:intron | 30.0% | |
| TATGATCCACGATATAAAAC+TGG | + | chr7.3:9499136-9499155 | None:intergenic | 30.0% | |
| TTGGAAAATGAACGAGTATA+AGG | - | chr7.3:9498759-9498778 | MS.gene050285:CDS | 30.0% | |
| ! | AGAACTATGGTTTTCTATAG+TGG | - | chr7.3:9498709-9498728 | MS.gene050285:CDS | 30.0% |
| ! | ATTATGATGCTTTGTTGGAT+TGG | - | chr7.3:9499544-9499563 | MS.gene050285:CDS | 30.0% |
| ! | CAATCAGTTTTCTTTCCATT+TGG | + | chr7.3:9498742-9498761 | None:intergenic | 30.0% |
| ! | GTGATTTTCATGGGAAAAAA+TGG | - | chr7.3:9499064-9499083 | MS.gene050285:intron | 30.0% |
| ! | TAATAAGGCGTGATTTTCAT+GGG | - | chr7.3:9499055-9499074 | MS.gene050285:intron | 30.0% |
| ! | TTATGATGCTTTGTTGGATT+GGG | - | chr7.3:9499545-9499564 | MS.gene050285:CDS | 30.0% |
| !! | AATGGTTCTTTTTCATTCCA+AGG | - | chr7.3:9498569-9498588 | MS.gene050285:CDS | 30.0% |
| AAGAGGATGTTTCATCTAAC+TGG | + | chr7.3:9498789-9498808 | None:intergenic | 35.0% | |
| ACATGATGTTCAAATCTCGA+TGG | - | chr7.3:9499425-9499444 | MS.gene050285:CDS | 35.0% | |
| CATTGGAGTGAAAAGAACTA+TGG | - | chr7.3:9498696-9498715 | MS.gene050285:CDS | 35.0% | |
| TCTTCAGATAATCGCATCAT+TGG | - | chr7.3:9498679-9498698 | MS.gene050285:CDS | 35.0% | |
| TTCAAACAAAGTAGCTCCTA+AGG | - | chr7.3:9498807-9498826 | MS.gene050285:CDS | 35.0% | |
| TTTACGGGGTCAATCTAATA+AGG | - | chr7.3:9499040-9499059 | MS.gene050285:intron | 35.0% | |
| TTTCATGGGAAAAAATGGCA+GGG | - | chr7.3:9499069-9499088 | MS.gene050285:intron | 35.0% | |
| ! | CTAATAAGGCGTGATTTTCA+TGG | - | chr7.3:9499054-9499073 | MS.gene050285:intron | 35.0% |
| ! | TGCTGATTATGATGCTTTGT+TGG | - | chr7.3:9499539-9499558 | MS.gene050285:CDS | 35.0% |
| ! | TTTTCATGGGAAAAAATGGC+AGG | - | chr7.3:9499068-9499087 | MS.gene050285:intron | 35.0% |
| !! | TGGAAACCAGTTTTATATCG+TGG | - | chr7.3:9499127-9499146 | MS.gene050285:intron | 35.0% |
| AACATGATTAGGAGATCCAG+TGG | + | chr7.3:9498656-9498675 | None:intergenic | 40.0% | |
| ACCCATGTTCAAAATGCTCA+AGG | - | chr7.3:9499378-9499397 | MS.gene050285:CDS | 40.0% | |
| CCCATGTTCAAAATGCTCAA+GGG | - | chr7.3:9499379-9499398 | MS.gene050285:CDS | 40.0% | |
| GGATGTTTCATCTAACTGGA+TGG | + | chr7.3:9498785-9498804 | None:intergenic | 40.0% | |
| ! | AGGAGCTACTTTGTTTGAAG+AGG | + | chr7.3:9498806-9498825 | None:intergenic | 40.0% |
| ! | CCCTTGAGCATTTTGAACAT+GGG | + | chr7.3:9499382-9499401 | None:intergenic | 40.0% |
| ! | GTTCGCAAGTTGAATCTTTC+TGG | + | chr7.3:9499407-9499426 | None:intergenic | 40.0% |
| ! | TATAGTGGAAGAGCTCCAAA+TGG | - | chr7.3:9498724-9498743 | MS.gene050285:CDS | 40.0% |
| !! | AGCAAGTGGATTGGTTCTTA+GGG | - | chr7.3:9499568-9499587 | MS.gene050285:CDS | 40.0% |
| AGAGGCAAGTATGAGAGACA+CGG | - | chr7.3:9498543-9498562 | MS.gene050285:CDS | 45.0% | |
| AGGCAAGAAAGTGAAGTGAG+AGG | - | chr7.3:9498589-9498608 | MS.gene050285:CDS | 45.0% | |
| CAAGAAAGTGAAGTGAGAGG+AGG | - | chr7.3:9498592-9498611 | MS.gene050285:CDS | 45.0% | |
| CTCACTTCACTTTCTTGCCT+TGG | + | chr7.3:9498589-9498608 | None:intergenic | 45.0% | |
| GGGTTGAACATGGTCTATAG+AGG | + | chr7.3:9499484-9499503 | None:intergenic | 45.0% | |
| GTATGAGAGACACGGAACAA+TGG | - | chr7.3:9498551-9498570 | MS.gene050285:CDS | 45.0% | |
| TCTATAGAGGAAGAGCTCTC+TGG | + | chr7.3:9499471-9499490 | None:intergenic | 45.0% | |
| ! | GCCCTTGAGCATTTTGAACA+TGG | + | chr7.3:9499383-9499402 | None:intergenic | 45.0% |
| ! | TTTGTTGGATTGGGAGCAAG+TGG | - | chr7.3:9499554-9499573 | MS.gene050285:CDS | 45.0% |
| !! | AGTGGATTGGTTCTTAGGGT+CGG | - | chr7.3:9499572-9499591 | MS.gene050285:CDS | 45.0% |
| !! | GAGCAAGTGGATTGGTTCTT+AGG | - | chr7.3:9499567-9499586 | MS.gene050285:CDS | 45.0% |
| AGCTTCTCCAACCTCTTGTG+AGG | + | chr7.3:9499505-9499524 | None:intergenic | 50.0% | |
| CCATGTTCAACCCTCACAAG+AGG | - | chr7.3:9499491-9499510 | MS.gene050285:CDS | 50.0% | |
| CCTCTTGTGAGGGTTGAACA+TGG | + | chr7.3:9499494-9499513 | None:intergenic | 50.0% | |
| GCTTCTCCAACCTCTTGTGA+GGG | + | chr7.3:9499504-9499523 | None:intergenic | 50.0% | |
| GGTTGGAGAAGCTAGCCAAA+TGG | - | chr7.3:9499512-9499531 | MS.gene050285:CDS | 50.0% | |
| GTTCAACCCTCACAAGAGGT+TGG | - | chr7.3:9499495-9499514 | MS.gene050285:CDS | 50.0% | |
| TTGATAGACGACCACCACCA+AGG | - | chr7.3:9499346-9499365 | MS.gene050285:CDS | 50.0% | |
| ! | ACATGGGTTGTAACCCTCCT+TGG | + | chr7.3:9499366-9499385 | None:intergenic | 50.0% |
| ! | CAAAGCGTCTCACCAACACT+GGG | - | chr7.3:9498620-9498639 | MS.gene050285:CDS | 50.0% |
| ! | TGGATTGGGAGCAAGTGGAT+TGG | - | chr7.3:9499559-9499578 | MS.gene050285:CDS | 50.0% |
| ATAGACGACCACCACCAAGG+AGG | - | chr7.3:9499349-9499368 | MS.gene050285:CDS | 55.0% | |
| GGCTTTCCAGTACCCAGTGT+TGG | + | chr7.3:9498635-9498654 | None:intergenic | 55.0% | |
| GTCTCACCAACACTGGGTAC+TGG | - | chr7.3:9498626-9498645 | MS.gene050285:CDS | 55.0% | |
| GTTGTAACCCTCCTTGGTGG+TGG | + | chr7.3:9499360-9499379 | None:intergenic | 55.0% | |
| TAGACGACCACCACCAAGGA+GGG | - | chr7.3:9499350-9499369 | MS.gene050285:CDS | 55.0% | |
| TGGGTTGTAACCCTCCTTGG+TGG | + | chr7.3:9499363-9499382 | None:intergenic | 55.0% | |
| ! | CCAAAGCGTCTCACCAACAC+TGG | - | chr7.3:9498619-9498638 | MS.gene050285:CDS | 55.0% |
| !! | ACTGGGTACTGGAAAGCCAC+TGG | - | chr7.3:9498637-9498656 | MS.gene050285:CDS | 55.0% |
| !! | CCAGTGTTGGTGAGACGCTT+TGG | + | chr7.3:9498622-9498641 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 9498533 | 9499602 | 9498533 | ID=MS.gene050285 |
| chr7.3 | mRNA | 9498533 | 9499602 | 9498533 | ID=MS.gene050285.t1;Parent=MS.gene050285 |
| chr7.3 | exon | 9499307 | 9499602 | 9499307 | ID=MS.gene050285.t1.exon1;Parent=MS.gene050285.t1 |
| chr7.3 | CDS | 9499307 | 9499602 | 9499307 | ID=cds.MS.gene050285.t1;Parent=MS.gene050285.t1 |
| chr7.3 | exon | 9498533 | 9498847 | 9498533 | ID=MS.gene050285.t1.exon2;Parent=MS.gene050285.t1 |
| chr7.3 | CDS | 9498533 | 9498847 | 9498533 | ID=cds.MS.gene050285.t1;Parent=MS.gene050285.t1 |
| Gene Sequence |
| Protein sequence |
