Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050366.t1 | XP_003611102.1 | 99.2 | 131 | 1 | 0 | 1 | 131 | 877 | 1007 | 1.20E-66 | 262.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050366.t1 | Q60EW9 | 76.3 | 131 | 31 | 0 | 1 | 131 | 644 | 774 | 2.4e-55 | 216.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050366.t1 | G7KC44 | 99.2 | 131 | 1 | 0 | 1 | 131 | 877 | 1007 | 8.6e-67 | 262.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050364 | MS.gene050366 | 0.914142 | 2.71E-84 | -1.69E-46 |
| MS.gene050366 | MS.gene050367 | 0.815921 | 7.21E-52 | -1.69E-46 |
| MS.gene050366 | MS.gene050369 | 0.905119 | 6.01E-80 | -1.69E-46 |
| MS.gene050366 | MS.gene072791 | 0.910763 | 1.30E-82 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050366.t1 | MTR_5g010390 | 99.237 | 131 | 1 | 0 | 1 | 131 | 877 | 1007 | 5.41e-86 | 270 |
| MS.gene050366.t1 | MTR_7g092770 | 75.573 | 131 | 32 | 0 | 1 | 131 | 645 | 775 | 1.92e-68 | 219 |
| MS.gene050366.t1 | MTR_0305s0020 | 76.336 | 131 | 31 | 0 | 1 | 131 | 644 | 774 | 2.41e-68 | 219 |
| MS.gene050366.t1 | MTR_4g066150 | 70.992 | 131 | 38 | 0 | 1 | 131 | 640 | 770 | 1.04e-65 | 212 |
| MS.gene050366.t1 | MTR_8g031270 | 70.992 | 131 | 38 | 0 | 1 | 131 | 873 | 1003 | 6.76e-65 | 212 |
| MS.gene050366.t1 | MTR_0291s0010 | 69.466 | 131 | 40 | 0 | 1 | 131 | 667 | 797 | 6.42e-64 | 207 |
| MS.gene050366.t1 | MTR_7g076900 | 68.702 | 131 | 41 | 0 | 1 | 131 | 883 | 1013 | 7.95e-61 | 201 |
| MS.gene050366.t1 | MTR_3g027150 | 68.702 | 131 | 41 | 0 | 1 | 131 | 692 | 822 | 8.94e-60 | 197 |
| MS.gene050366.t1 | MTR_4g023460 | 67.176 | 131 | 43 | 0 | 1 | 131 | 1012 | 1142 | 9.54e-60 | 198 |
| MS.gene050366.t1 | MTR_4g033215 | 63.359 | 131 | 48 | 0 | 1 | 131 | 914 | 1044 | 4.24e-59 | 196 |
| MS.gene050366.t1 | MTR_4g023250 | 60.769 | 130 | 49 | 1 | 1 | 130 | 1 | 128 | 3.94e-55 | 169 |
| MS.gene050366.t1 | MTR_4g107850 | 63.359 | 131 | 48 | 0 | 1 | 131 | 908 | 1038 | 1.34e-54 | 183 |
| MS.gene050366.t1 | MTR_6g027540 | 54.962 | 131 | 59 | 0 | 1 | 131 | 879 | 1009 | 4.23e-49 | 167 |
| MS.gene050366.t1 | MTR_6g028050 | 54.688 | 128 | 58 | 0 | 4 | 131 | 899 | 1026 | 6.34e-47 | 161 |
| MS.gene050366.t1 | MTR_4g057675 | 48.092 | 131 | 68 | 0 | 1 | 131 | 653 | 783 | 4.23e-36 | 130 |
| MS.gene050366.t1 | MTR_4g071870 | 51.000 | 100 | 49 | 0 | 32 | 131 | 650 | 749 | 2.59e-32 | 120 |
| MS.gene050366.t1 | MTR_1g015540 | 38.931 | 131 | 80 | 0 | 1 | 131 | 643 | 773 | 3.59e-27 | 105 |
| MS.gene050366.t1 | MTR_5g096980 | 41.085 | 129 | 74 | 2 | 4 | 131 | 636 | 763 | 2.23e-25 | 100 |
| MS.gene050366.t1 | MTR_4g023390 | 44.231 | 104 | 36 | 2 | 4 | 107 | 16 | 97 | 3.87e-23 | 86.7 |
| MS.gene050366.t1 | MTR_4g023230 | 64.103 | 39 | 14 | 0 | 1 | 39 | 117 | 155 | 1.05e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050366.t1 | AT4G11610 | 75.573 | 131 | 32 | 0 | 1 | 131 | 744 | 874 | 8.55e-70 | 224 |
| MS.gene050366.t1 | AT4G11610 | 75.573 | 131 | 32 | 0 | 1 | 131 | 881 | 1011 | 2.75e-69 | 224 |
| MS.gene050366.t1 | AT4G11610 | 75.573 | 131 | 32 | 0 | 1 | 131 | 877 | 1007 | 2.80e-69 | 224 |
| MS.gene050366.t1 | AT5G12970 | 73.282 | 131 | 35 | 0 | 1 | 131 | 639 | 769 | 3.19e-67 | 216 |
| MS.gene050366.t1 | AT3G57880 | 74.809 | 131 | 33 | 0 | 1 | 131 | 643 | 773 | 8.07e-67 | 215 |
| MS.gene050366.t1 | AT3G57880 | 74.809 | 131 | 33 | 0 | 1 | 131 | 643 | 773 | 8.07e-67 | 215 |
| MS.gene050366.t1 | AT3G57880 | 74.809 | 131 | 33 | 0 | 1 | 131 | 643 | 773 | 8.07e-67 | 215 |
| MS.gene050366.t1 | AT3G57880 | 74.809 | 131 | 33 | 0 | 1 | 131 | 643 | 773 | 8.07e-67 | 215 |
| MS.gene050366.t1 | AT1G51570 | 73.282 | 131 | 35 | 0 | 1 | 131 | 646 | 776 | 9.70e-66 | 212 |
| MS.gene050366.t1 | AT4G00700 | 67.939 | 131 | 42 | 0 | 1 | 131 | 876 | 1006 | 3.14e-63 | 207 |
| MS.gene050366.t1 | AT4G00700 | 67.939 | 131 | 42 | 0 | 1 | 131 | 876 | 1006 | 3.14e-63 | 207 |
| MS.gene050366.t1 | AT5G06850 | 67.176 | 131 | 43 | 0 | 1 | 131 | 664 | 794 | 1.65e-62 | 204 |
| MS.gene050366.t1 | AT1G22610 | 69.466 | 131 | 40 | 0 | 1 | 131 | 899 | 1029 | 2.03e-61 | 202 |
| MS.gene050366.t1 | AT5G48060 | 67.939 | 131 | 42 | 0 | 1 | 131 | 906 | 1036 | 4.46e-60 | 199 |
| MS.gene050366.t1 | AT1G04150 | 64.885 | 131 | 46 | 0 | 1 | 131 | 882 | 1012 | 4.45e-57 | 190 |
| MS.gene050366.t1 | AT3G61300 | 64.394 | 132 | 46 | 1 | 1 | 131 | 841 | 972 | 1.95e-56 | 188 |
| MS.gene050366.t1 | AT3G03680 | 56.489 | 131 | 57 | 0 | 1 | 131 | 887 | 1017 | 7.66e-53 | 178 |
| MS.gene050366.t1 | AT1G74720 | 58.779 | 131 | 54 | 0 | 1 | 131 | 951 | 1081 | 7.95e-52 | 176 |
| MS.gene050366.t1 | AT4G20080 | 56.061 | 132 | 57 | 1 | 1 | 131 | 643 | 774 | 6.15e-47 | 161 |
| MS.gene050366.t1 | AT5G17980 | 52.308 | 130 | 62 | 0 | 2 | 131 | 920 | 1049 | 9.94e-47 | 161 |
| MS.gene050366.t1 | AT3G61720 | 35.766 | 137 | 82 | 3 | 1 | 131 | 659 | 795 | 7.70e-22 | 90.1 |
| MS.gene050366.t1 | AT5G03435 | 42.105 | 114 | 63 | 2 | 14 | 124 | 626 | 739 | 2.59e-21 | 88.6 |
Find 0 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATGTTACTCCTTTTCAAA+TGG | - | 38013:6936-6955 | MS.gene050366:CDS | 25.0% |
| AAATTCCTCATCCATTTCAT+CGG | + | 38013:6719-6738 | None:intergenic | 30.0% | |
| AACATACTATCAGTTCTTGC+AGG | + | 38013:7051-7070 | None:intergenic | 35.0% | |
| AATTCCTCATCCATTTCATC+GGG | + | 38013:6718-6737 | None:intergenic | 35.0% | |
| ATGAATAAAGAAGTAGCACG+CGG | + | 38013:6886-6905 | MS.gene050366:intergenic | 35.0% | |
| TAGCAGGTTTCTATTTCATG+AGG | - | 38013:6968-6987 | MS.gene050366:CDS | 35.0% | |
| ! | TGTTACTCCTTTTCAAATGG+TGG | - | 38013:6939-6958 | MS.gene050366:CDS | 35.0% |
| ! | TTTCTGTTTGTTAGCTGCTT+TGG | - | 38013:6909-6928 | MS.gene050366:CDS | 35.0% |
| AAACCAGCCACCATTTGAAA+AGG | + | 38013:6949-6968 | None:intergenic | 40.0% | |
| ACAAACGGTTGTAGGTGATT+TGG | - | 38013:6816-6835 | MS.gene050366:CDS | 40.0% | |
| ATCCTGACCTTGTAAGAATG+CGG | - | 38013:6761-6780 | MS.gene050366:CDS | 40.0% | |
| ATTCCTCATCCATTTCATCG+GGG | + | 38013:6717-6736 | MS.gene050366:intergenic | 40.0% | |
| GATCATACCGCATTCTTACA+AGG | + | 38013:6771-6790 | MS.gene050366:intergenic | 40.0% | |
| GGTAGAATACAAACGGTTGT+AGG | - | 38013:6808-6827 | MS.gene050366:CDS | 40.0% | |
| ! | AGGTCAGGATTTTTGCTTGT+TGG | + | 38013:6751-6770 | MS.gene050366:intergenic | 40.0% |
| CGTGGCTGGTAGAATACAAA+CGG | - | 38013:6801-6820 | MS.gene050366:CDS | 45.0% | |
| GAATGCGGTATGATCGTTTG+AGG | - | 38013:6776-6795 | MS.gene050366:CDS | 45.0% | |
| GGAAGCCTGTTTCGAAATCT+GGG | + | 38013:6997-7016 | MS.gene050366:intergenic | 45.0% | |
| TACCGCATTCTTACAAGGTC+AGG | + | 38013:6766-6785 | MS.gene050366:intergenic | 45.0% | |
| TGTAGTACACCCCGATGAAA+TGG | - | 38013:6705-6724 | MS.gene050366:CDS | 45.0% | |
| ! | AAGAAGTTGATCGGTGCAGA+CGG | + | 38013:7018-7037 | MS.gene050366:intergenic | 45.0% |
| ! | ACTCCTTTTCAAATGGTGGC+TGG | - | 38013:6943-6962 | MS.gene050366:CDS | 45.0% |
| ! | AGTCGACGGAAGAAGTTGAT+CGG | + | 38013:7027-7046 | MS.gene050366:intergenic | 45.0% |
| ACACCCCGATGAAATGGATG+AGG | - | 38013:6711-6730 | MS.gene050366:CDS | 50.0% | |
| CAGTTCTTGCAGGAAGTCGA+CGG | + | 38013:7041-7060 | MS.gene050366:intergenic | 50.0% | |
| CGGAAGCCTGTTTCGAAATC+TGG | + | 38013:6998-7017 | MS.gene050366:intergenic | 50.0% | |
| GGATTCATGCACTGTTGAGC+TGG | - | 38013:6854-6873 | MS.gene050366:CDS | 50.0% | |
| GGCATCCCAGATTTCGAAAC+AGG | - | 38013:6989-7008 | MS.gene050366:CDS | 50.0% | |
| GTATGATCGTTTGAGGAGCG+TGG | - | 38013:6783-6802 | MS.gene050366:CDS | 50.0% | |
| ! | GTAGGTGATTTGGCTAGCCA+GGG | - | 38013:6826-6845 | MS.gene050366:CDS | 50.0% |
| ! | TGTAGGTGATTTGGCTAGCC+AGG | - | 38013:6825-6844 | MS.gene050366:CDS | 50.0% |
| !! | CAAATGGTGGCTGGTTTAGC+AGG | - | 38013:6952-6971 | MS.gene050366:CDS | 50.0% |
| GTGCATGAATCCTCTCACCC+TGG | + | 38013:6846-6865 | MS.gene050366:intergenic | 55.0% | |
| ! | ATTTGGCTAGCCAGGGTGAG+AGG | - | 38013:6833-6852 | MS.gene050366:CDS | 55.0% |
| ! | GATCGTTTGAGGAGCGTGGC+TGG | - | 38013:6787-6806 | MS.gene050366:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 38013 | gene | 6679 | 7074 | 6679 | ID=MS.gene050366 |
| 38013 | mRNA | 6679 | 7074 | 6679 | ID=MS.gene050366.t1;Parent=MS.gene050366 |
| 38013 | exon | 6679 | 7074 | 6679 | ID=MS.gene050366.t1.exon1;Parent=MS.gene050366.t1 |
| 38013 | CDS | 6679 | 7074 | 6679 | ID=cds.MS.gene050366.t1;Parent=MS.gene050366.t1 |
| Gene Sequence |
| Protein sequence |
