AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072700


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072700.t1 MTR_7g109510 82.781 151 12 2 1 151 1 137 6.79e-75 220
MS.gene072700.t1 MTR_1g087040 52.055 146 60 3 1 140 1 142 1.04e-39 131
MS.gene072700.t1 MTR_4g070860 48.387 93 47 1 10 101 17 109 1.11e-19 80.5
MS.gene072700.t1 MTR_3g117120 53.012 83 39 0 19 101 27 109 1.13e-19 80.5
MS.gene072700.t1 MTR_1g022495 54.217 83 38 0 19 101 27 109 1.36e-17 75.1
MS.gene072700.t1 MTR_8g086020 40.594 101 55 1 10 105 18 118 9.00e-16 70.5
MS.gene072700.t1 MTR_5g015090 52.857 70 24 1 2 62 32 101 5.31e-13 63.2
MS.gene072700.t1 MTR_4g097440 39.823 113 40 3 7 94 45 154 7.37e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072700.t1 AT3G62420 46.711 152 70 3 1 149 1 144 8.78e-35 119
MS.gene072700.t1 AT2G18160 44.118 102 52 1 18 119 27 123 1.06e-20 83.6
MS.gene072700.t1 AT1G75390 38.849 139 72 3 19 149 38 171 1.30e-20 83.2
MS.gene072700.t1 AT4G34590 48.810 84 43 0 19 102 24 107 1.32e-19 80.5
MS.gene072700.t1 AT1G75390 49.254 67 34 0 19 85 38 104 4.22e-12 59.7

Find 42 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCCGAATCAAGCGATTA+AGG 0.283521 7.3:-5465190 MS.gene072700:CDS
GGCGATTGTTGGAGAACATT+AGG 0.296251 7.3:-5465413 MS.gene072700:CDS
CAGATTGGTTCTTGCATTGA+TGG 0.301394 7.3:-5465155 None:intergenic
GGTTCAACGTGTGACGAGTT+CGG 0.348908 7.3:-5465583 MS.gene072700:CDS
GAGCGATGGACGAGAGAAAA+AGG 0.360427 7.3:-5465542 MS.gene072700:CDS
GTTCAACGTGTGACGAGTTC+GGG 0.369000 7.3:-5465582 MS.gene072700:CDS
TGAATCAATTTGAATGGCTT+CGG 0.385700 7.3:-5465604 None:intergenic
GCGATTGTTGGAGAACATTA+GGG 0.409944 7.3:-5465412 MS.gene072700:CDS
CACGGGTTCAGTTGTGGATC+TGG 0.419548 7.3:+5465222 None:intergenic
GTGATCGCCGTGCGGATTCA+CGG 0.422997 7.3:+5465502 None:intergenic
ATCTTCAAGATGAGGCAAAC+AGG 0.431346 7.3:-5465452 MS.gene072700:CDS
AGTTGTGGATCTGGAATCTT+CGG 0.445106 7.3:+5465231 None:intergenic
AAACGAGAATCGGCGATTGT+TGG 0.446153 7.3:-5465424 MS.gene072700:CDS
AATGCAAACGAAGCCGCTAA+TGG 0.446683 7.3:-5465372 MS.gene072700:CDS
AACATTAGGGCGAGGGAAGA+AGG 0.454886 7.3:-5465399 MS.gene072700:CDS
ACGTGTGACGAGTTCGGGTT+CGG 0.460336 7.3:-5465577 MS.gene072700:CDS
GACGCCTTAATCGCTTGATT+CGG 0.461919 7.3:+5465186 None:intergenic
AGGCGTCTTCGTCTTCAGAT+TGG 0.488511 7.3:-5465170 MS.gene072700:CDS
TGTCCTCGGAGCTCGGATAC+AGG 0.488969 7.3:-5465349 MS.gene072700:CDS
AAAGCTGAGAGGGAAAAGAA+TGG 0.490064 7.3:-5465270 MS.gene072700:CDS
TCGGGTTCGGACGGTGTGAA+TGG 0.491849 7.3:-5465564 MS.gene072700:CDS
GTGAATCCGCACGGCGATCA+CGG 0.512811 7.3:-5465500 MS.gene072700:CDS
CTAATGGTGTCCTCGGAGCT+CGG 0.524737 7.3:-5465356 MS.gene072700:CDS
TGAATCCGCACGGCGATCAC+GGG 0.531059 7.3:-5465499 MS.gene072700:CDS
ACAGGTTGCGAAACGAGAAT+CGG 0.542037 7.3:-5465434 MS.gene072700:CDS
CCGCACGGCGATCACGGGAG+AGG 0.550454 7.3:-5465494 MS.gene072700:CDS
TGTTGGAGAACATTAGGGCG+AGG 0.554484 7.3:-5465407 MS.gene072700:CDS
ATCTGCCACGGGTTCAGTTG+TGG 0.561631 7.3:+5465216 None:intergenic
AGCTCCGAGGACACCATTAG+CGG 0.564090 7.3:+5465359 None:intergenic
GAAGCCGCTAATGGTGTCCT+CGG 0.572523 7.3:-5465363 MS.gene072700:CDS
TCGGACAAAGCATCTGCCAC+GGG 0.574386 7.3:+5465205 None:intergenic
GTTGGAGAACATTAGGGCGA+GGG 0.586730 7.3:-5465406 MS.gene072700:CDS
GGAGAGGAAGCAGAAACTGA+TGG 0.591146 7.3:-5465478 MS.gene072700:CDS
TTCGGACAAAGCATCTGCCA+CGG 0.597740 7.3:+5465204 None:intergenic
GGACGGTGTGAATGGAGCGA+TGG 0.618123 7.3:-5465556 MS.gene072700:CDS
GTGACGAGTTCGGGTTCGGA+CGG 0.621958 7.3:-5465573 MS.gene072700:CDS
GATGGAGGATCTTCAAGATG+AGG 0.635369 7.3:-5465460 MS.gene072700:CDS
CAGATCCACAACTGAACCCG+TGG 0.655694 7.3:-5465221 MS.gene072700:CDS
TTTCGAACCGTGAATCCGCA+CGG 0.677805 7.3:-5465509 MS.gene072700:CDS
CCTCTCCCGTGATCGCCGTG+CGG 0.684095 7.3:+5465494 None:intergenic
GAGGAAGCAGAAACTGATGG+AGG 0.689697 7.3:-5465475 MS.gene072700:CDS
ATTCCTGTATCCGAGCTCCG+AGG 0.746883 7.3:+5465346 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGCTGAGAGGGAAAAGAA+TGG - chr7.3:5465479-5465498 MS.gene072700:CDS 40.0%
ATCTTCAAGATGAGGCAAAC+AGG - chr7.3:5465297-5465316 MS.gene072700:CDS 40.0%
GCGATTGTTGGAGAACATTA+GGG - chr7.3:5465337-5465356 MS.gene072700:CDS 40.0%
TGAGAGAGAAAAAGCTGAGA+GGG - chr7.3:5465469-5465488 MS.gene072700:CDS 40.0%
TTGTCCGAATCAAGCGATTA+AGG - chr7.3:5465559-5465578 MS.gene072700:CDS 40.0%
! AGTTGTGGATCTGGAATCTT+CGG + chr7.3:5465521-5465540 None:intergenic 40.0%
AAACGAGAATCGGCGATTGT+TGG - chr7.3:5465325-5465344 MS.gene072700:CDS 45.0%
AATGCAAACGAAGCCGCTAA+TGG - chr7.3:5465377-5465396 MS.gene072700:CDS 45.0%
ACAGGTTGCGAAACGAGAAT+CGG - chr7.3:5465315-5465334 MS.gene072700:CDS 45.0%
CTGAGAGAGAAAAAGCTGAG+AGG - chr7.3:5465468-5465487 MS.gene072700:CDS 45.0%
GACGCCTTAATCGCTTGATT+CGG + chr7.3:5465566-5465585 None:intergenic 45.0%
GATGGAGGATCTTCAAGATG+AGG - chr7.3:5465289-5465308 MS.gene072700:CDS 45.0%
GGCGATTGTTGGAGAACATT+AGG - chr7.3:5465336-5465355 MS.gene072700:CDS 45.0%
AACATTAGGGCGAGGGAAGA+AGG - chr7.3:5465350-5465369 MS.gene072700:CDS 50.0%
AGGCGTCTTCGTCTTCAGAT+TGG - chr7.3:5465579-5465598 MS.gene072700:CDS 50.0%
GAGCGATGGACGAGAGAAAA+AGG - chr7.3:5465207-5465226 MS.gene072700:CDS 50.0%
GAGGAAGCAGAAACTGATGG+AGG - chr7.3:5465274-5465293 MS.gene072700:CDS 50.0%
GGAGAGGAAGCAGAAACTGA+TGG - chr7.3:5465271-5465290 MS.gene072700:CDS 50.0%
GGTTCAACGTGTGACGAGTT+CGG - chr7.3:5465166-5465185 MS.gene072700:CDS 50.0%
GTTCAACGTGTGACGAGTTC+GGG - chr7.3:5465167-5465186 MS.gene072700:CDS 50.0%
GTTGGAGAACATTAGGGCGA+GGG - chr7.3:5465343-5465362 MS.gene072700:CDS 50.0%
TGTTGGAGAACATTAGGGCG+AGG - chr7.3:5465342-5465361 MS.gene072700:CDS 50.0%
TTTCGAACCGTGAATCCGCA+CGG - chr7.3:5465240-5465259 MS.gene072700:CDS 50.0%
! TTCGGACAAAGCATCTGCCA+CGG + chr7.3:5465548-5465567 None:intergenic 50.0%
AGCTCCGAGGACACCATTAG+CGG + chr7.3:5465393-5465412 None:intergenic 55.0%
ATCTGCCACGGGTTCAGTTG+TGG + chr7.3:5465536-5465555 None:intergenic 55.0%
ATTCCTGTATCCGAGCTCCG+AGG + chr7.3:5465406-5465425 None:intergenic 55.0%
CACGGGTTCAGTTGTGGATC+TGG + chr7.3:5465530-5465549 None:intergenic 55.0%
CAGATCCACAACTGAACCCG+TGG - chr7.3:5465528-5465547 MS.gene072700:CDS 55.0%
CTAATGGTGTCCTCGGAGCT+CGG - chr7.3:5465393-5465412 MS.gene072700:CDS 55.0%
GAAGCCGCTAATGGTGTCCT+CGG - chr7.3:5465386-5465405 MS.gene072700:CDS 55.0%
! ACGTGTGACGAGTTCGGGTT+CGG - chr7.3:5465172-5465191 MS.gene072700:CDS 55.0%
! TCGGACAAAGCATCTGCCAC+GGG + chr7.3:5465547-5465566 None:intergenic 55.0%
GGACGGTGTGAATGGAGCGA+TGG - chr7.3:5465193-5465212 MS.gene072700:CDS 60.0%
GTGAATCCGCACGGCGATCA+CGG - chr7.3:5465249-5465268 MS.gene072700:CDS 60.0%
GTGATCGCCGTGCGGATTCA+CGG + chr7.3:5465250-5465269 None:intergenic 60.0%
TCGGGTTCGGACGGTGTGAA+TGG - chr7.3:5465185-5465204 MS.gene072700:CDS 60.0%
TGAATCCGCACGGCGATCAC+GGG - chr7.3:5465250-5465269 MS.gene072700:CDS 60.0%
TGTCCTCGGAGCTCGGATAC+AGG - chr7.3:5465400-5465419 MS.gene072700:CDS 60.0%
! GTGACGAGTTCGGGTTCGGA+CGG - chr7.3:5465176-5465195 MS.gene072700:CDS 60.0%
CCTCTCCCGTGATCGCCGTG+CGG + chr7.3:5465258-5465277 None:intergenic 70.0%
CCGCACGGCGATCACGGGAG+AGG - chr7.3:5465255-5465274 MS.gene072700:CDS 75.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 5465158 5465613 5465158 ID=MS.gene072700
chr7.3 mRNA 5465158 5465613 5465158 ID=MS.gene072700.t1;Parent=MS.gene072700
chr7.3 exon 5465158 5465613 5465158 ID=MS.gene072700.t1.exon1;Parent=MS.gene072700.t1
chr7.3 CDS 5465158 5465613 5465158 ID=cds.MS.gene072700.t1;Parent=MS.gene072700.t1
Gene Sequence

>MS.gene072700

ATGGCTTCGGTTCAACGTGTGACGAGTTCGGGTTCGGACGGTGTGAATGGAGCGATGGACGAGAGAAAAAGGAAGAGAATGATTTCGAACCGTGAATCCGCACGGCGATCACGGGAGAGGAAGCAGAAACTGATGGAGGATCTTCAAGATGAGGCAAACAGGTTGCGAAACGAGAATCGGCGATTGTTGGAGAACATTAGGGCGAGGGAAGAAGGTTTCAATGCAAACGAAGCCGCTAATGGTGTCCTCGGAGCTCGGATACAGGAATTGACAGATCAATTGAAGTTTCTGAACTCAATCATTGAAAAAGCTGAGAGAGAAAAAGCTGAGAGGGAAAAGAATGGATTTTCTGTTGAGATTCCGAAGATTCCAGATCCACAACTGAACCCGTGGCAGATGCTTTGTCCGAATCAAGCGATTAAGGCGTCTTCGTCTTCAGATTGGTTCTTGCATTGA

Protein sequence

>MS.gene072700.t1

MASVQRVTSSGSDGVNGAMDERKRKRMISNRESARRSRERKQKLMEDLQDEANRLRNENRRLLENIRAREEGFNANEAANGVLGARIQELTDQLKFLNSIIEKAEREKAEREKNGFSVEIPKIPDPQLNPWQMLCPNQAIKASSSSDWFLH