AlfalfaGEDB | MS.gene050447

M. sativa cultivar XinJiangDaYe / MS.gene050447

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050447.t1	XP_003625987.2	99.2	118	1	0	1	118	1020	1137	8.30E-59	236.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050447.t1	G7KTM5	99.2	118	1	0	1	118	1020	1137	6.0e-59	236.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050447	MS.gene050448	0.991621	1.25E-188	-1.69E-46
MS.gene050447	MS.gene32700	0.828705	7.75E-55	-1.69E-46
MS.gene050447	MS.gene42606	0.820972	5.17E-53	-1.69E-46
MS.gene050447	MS.gene42607	0.821912	3.14E-53	-1.69E-46
MS.gene050447	MS.gene42612	0.822579	2.20E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050447.t1	MTR_7g109730	99.153	118	1	0	1	118	1020	1137	9.77e-75	240

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050447.t1	AT3G62360	61.607	112	43	0	1	112	1020	1131	2.56e-43	150
MS.gene050447.t1	AT3G62360	61.607	112	43	0	1	112	1050	1161	2.64e-43	150

Find 33 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTGAGGATATCAAGGGTT+TGG	0.335214	7.2:+6077150	MS.gene050447:CDS
ACTTGGAAGAAGTTCGAAAT+TGG	0.340693	7.2:-6077316	None:intergenic
CAACTTACAATTGTCATATC+TGG	0.370044	7.2:-6077193	None:intergenic
ATTGTAAGTTGTCATGTTGA+AGG	0.424855	7.2:+6077205	MS.gene050447:CDS
AACATCTGATGGTAGAAATC+AGG	0.427976	7.2:+6077251	MS.gene050447:CDS
ATGTGGGCCCACTGAGATAC+AGG	0.441245	7.2:+6077455	MS.gene050447:CDS
GGAAGAAGTTCGAAATTGGC+AGG	0.447899	7.2:-6077312	None:intergenic
TGTATCTCAGTGGGCCCACA+TGG	0.456400	7.2:-6077453	None:intergenic
CAAATCTAAGTGAGCTTGAT+GGG	0.474246	7.2:-6077387	None:intergenic
TTCGAACTTCTTCCAAGTGA+AGG	0.478479	7.2:+6077321	MS.gene050447:CDS
TCAAATCTAAGTGAGCTTGA+TGG	0.486532	7.2:-6077388	None:intergenic
GACCAGATCGAAGTTGTAGA+AGG	0.489153	7.2:-6077363	None:intergenic
GGTTGGAACTGAGGATATCA+AGG	0.505962	7.2:+6077144	None:intergenic
GATTGATGATCAGCTGAAGC+AGG	0.507779	7.2:+6077477	MS.gene050447:CDS
AGTTGTCATGTTGAAGGAAA+CGG	0.513703	7.2:+6077211	MS.gene050447:CDS
TCGAACTTCTTCCAAGTGAA+GGG	0.516414	7.2:+6077322	MS.gene050447:CDS
CACCTTCTACAACTTCGATC+TGG	0.522791	7.2:+6077361	MS.gene050447:CDS
TGAAGGGCTTATCGAAGGGC+AGG	0.529646	7.2:+6077338	MS.gene050447:CDS
AAGAAGTTCGAAATTGGCAG+GGG	0.530875	7.2:-6077310	None:intergenic
TGATATCATTGAGGTCGACT+TGG	0.534767	7.2:+6077414	MS.gene050447:CDS
ATTTGAATCTGATATCATTG+AGG	0.563041	7.2:+6077405	MS.gene050447:CDS
CCTTCGATAAGCCCTTCACT+TGG	0.563336	7.2:-6077333	None:intergenic
CCAAGTGAAGGGCTTATCGA+AGG	0.581387	7.2:+6077333	MS.gene050447:CDS
GAAGAAGTTCGAAATTGGCA+GGG	0.582032	7.2:-6077311	None:intergenic
ATGTTTCCAGGTTGGAACTG+AGG	0.588059	7.2:+6077135	None:intergenic
CATCAATCCTGTATCTCAGT+GGG	0.588309	7.2:-6077462	None:intergenic
TGAGCTTCGTAAACATCTGA+TGG	0.589751	7.2:+6077240	MS.gene050447:CDS
TCATGTTGAAGGAAACGGTA+CGG	0.617690	7.2:+6077216	MS.gene050447:CDS
GTATCTCAGTGGGCCCACAT+GGG	0.619772	7.2:-6077452	None:intergenic
CAAGTGAAGGGCTTATCGAA+GGG	0.624792	7.2:+6077334	MS.gene050447:CDS
GTTGGAACTGAGGATATCAA+GGG	0.626948	7.2:+6077145	MS.gene050447:CDS
TCATCAATCCTGTATCTCAG+TGG	0.679879	7.2:-6077463	None:intergenic
AGAAGTTCGAAATTGGCAGG+GGG	0.705475	7.2:-6077309	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGGATTTTATAGTTTTTG+AGG	+	chr7.2:6077168-6077187	MS.gene050447:CDS	20.0%
!	ATTTGAATCTGATATCATTG+AGG	+	chr7.2:6077405-6077424	MS.gene050447:CDS	25.0%
	AAAAATGTTCAAACCCATGT+GGG	+	chr7.2:6077439-6077458	MS.gene050447:CDS	30.0%
	ATTGTAAGTTGTCATGTTGA+AGG	+	chr7.2:6077205-6077224	MS.gene050447:CDS	30.0%
	CAACTTACAATTGTCATATC+TGG	-	chr7.2:6077196-6077215	None:intergenic	30.0%
	AACATCTGATGGTAGAAATC+AGG	+	chr7.2:6077251-6077270	MS.gene050447:CDS	35.0%
	ACTTGGAAGAAGTTCGAAAT+TGG	-	chr7.2:6077319-6077338	None:intergenic	35.0%
	AGTTGTCATGTTGAAGGAAA+CGG	+	chr7.2:6077211-6077230	MS.gene050447:CDS	35.0%
	CAAAAATGTTCAAACCCATG+TGG	+	chr7.2:6077438-6077457	MS.gene050447:CDS	35.0%
	CAAATCTAAGTGAGCTTGAT+GGG	-	chr7.2:6077390-6077409	None:intergenic	35.0%
	TCAAATCTAAGTGAGCTTGA+TGG	-	chr7.2:6077391-6077410	None:intergenic	35.0%
	AACTGAGGATATCAAGGGTT+TGG	+	chr7.2:6077150-6077169	MS.gene050447:CDS	40.0%
	AAGAAGTTCGAAATTGGCAG+GGG	-	chr7.2:6077313-6077332	None:intergenic	40.0%
	CATCAATCCTGTATCTCAGT+GGG	-	chr7.2:6077465-6077484	None:intergenic	40.0%
	GAAGAAGTTCGAAATTGGCA+GGG	-	chr7.2:6077314-6077333	None:intergenic	40.0%
	GTTGGAACTGAGGATATCAA+GGG	+	chr7.2:6077145-6077164	MS.gene050447:CDS	40.0%
	TCATCAATCCTGTATCTCAG+TGG	-	chr7.2:6077466-6077485	None:intergenic	40.0%
	TCATGTTGAAGGAAACGGTA+CGG	+	chr7.2:6077216-6077235	MS.gene050447:CDS	40.0%
	TCGAACTTCTTCCAAGTGAA+GGG	+	chr7.2:6077322-6077341	MS.gene050447:CDS	40.0%
	TGAGCTTCGTAAACATCTGA+TGG	+	chr7.2:6077240-6077259	MS.gene050447:CDS	40.0%
	TGATATCATTGAGGTCGACT+TGG	+	chr7.2:6077414-6077433	MS.gene050447:CDS	40.0%
	TTCGAACTTCTTCCAAGTGA+AGG	+	chr7.2:6077321-6077340	MS.gene050447:CDS	40.0%
	AGAAGTTCGAAATTGGCAGG+GGG	-	chr7.2:6077312-6077331	None:intergenic	45.0%
	CAAGTGAAGGGCTTATCGAA+GGG	+	chr7.2:6077334-6077353	MS.gene050447:CDS	45.0%
	CACCTTCTACAACTTCGATC+TGG	+	chr7.2:6077361-6077380	MS.gene050447:CDS	45.0%
	GACCAGATCGAAGTTGTAGA+AGG	-	chr7.2:6077366-6077385	None:intergenic	45.0%
	GGAAGAAGTTCGAAATTGGC+AGG	-	chr7.2:6077315-6077334	None:intergenic	45.0%
!	GATTGATGATCAGCTGAAGC+AGG	+	chr7.2:6077477-6077496	MS.gene050447:CDS	45.0%
	CCAAGTGAAGGGCTTATCGA+AGG	+	chr7.2:6077333-6077352	MS.gene050447:CDS	50.0%
	CCTTCGATAAGCCCTTCACT+TGG	-	chr7.2:6077336-6077355	None:intergenic	50.0%
	ATGTGGGCCCACTGAGATAC+AGG	+	chr7.2:6077455-6077474	MS.gene050447:CDS	55.0%
	GTATCTCAGTGGGCCCACAT+GGG	-	chr7.2:6077455-6077474	None:intergenic	55.0%
	TGAAGGGCTTATCGAAGGGC+AGG	+	chr7.2:6077338-6077357	MS.gene050447:CDS	55.0%
	TGTATCTCAGTGGGCCCACA+TGG	-	chr7.2:6077456-6077475	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	6077145	6077498	6077145	ID=MS.gene050447
chr7.2	mRNA	6077145	6077498	6077145	ID=MS.gene050447.t1;Parent=MS.gene050447
chr7.2	exon	6077145	6077498	6077145	ID=MS.gene050447.t1.exon1;Parent=MS.gene050447.t1
chr7.2	CDS	6077145	6077498	6077145	ID=cds.MS.gene050447.t1;Parent=MS.gene050447.t1

Gene Sequence	>MS.gene050447 GTTGGAACTGAGGATATCAAGGGTTTGGATTTTATAGTTTTTGAGGAGCCAGATATGACAATTGTAAGTTGTCATGTTGAAGGAAACGGTACGGATGAGCTTCGTAAACATCTGATGGTAGAAATCAGGTCTGCTAGTGAGACAAGCAAGATAGAGTCAGTCTTCCCCCTGCCAATTTCGAACTTCTTCCAAGTGAAGGGCTTATCGAAGGGCAGGCACCTTCTACAACTTCGATCTGGTCTCCCATCAAGCTCACTTAGATTTGAATCTGATATCATTGAGGTCGACTTGGACAAAAATGTTCAAACCCATGTGGGCCCACTGAGATACAGGATTGATGATCAGCTGAAGCAG
Protein sequence	>MS.gene050447.t1 VGTEDIKGLDFIVFEEPDMTIVSCHVEGNGTDELRKHLMVEIRSASETSKIESVFPLPISNFFQVKGLSKGRHLLQLRSGLPSSSLRFESDIIEVDLDKNVQTHVGPLRYRIDDQLKQ