AlfalfaGEDB | MS.gene050448

M. sativa cultivar XinJiangDaYe / MS.gene050448

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050448.t1	XP_003625987.2	99.2	118	1	0	1	118	1020	1137	8.30E-59	236.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050448.t1	G7KTM5	99.2	118	1	0	1	118	1020	1137	6.0e-59	236.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050447	MS.gene050448	0.991621	1.25E-188	-1.69E-46
MS.gene050448	MS.gene32700	0.83766	4.56E-57	-1.69E-46
MS.gene050448	MS.gene42606	0.830121	3.51E-55	-1.69E-46
MS.gene050448	MS.gene42607	0.83326	5.90E-56	-1.69E-46
MS.gene050448	MS.gene42612	0.832216	1.07E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050448.t1	MTR_7g109730	99.153	118	1	0	1	118	1020	1137	9.77e-75	240

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050448.t1	AT3G62360	61.607	112	43	0	1	112	1020	1131	2.56e-43	150
MS.gene050448.t1	AT3G62360	61.607	112	43	0	1	112	1050	1161	2.64e-43	150

Find 33 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTGAGGATATCAAGGGTT+TGG	0.335214	7.2:+5955881	MS.gene050448:CDS
ACTTGGAAGAAGTTCGAAAT+TGG	0.340693	7.2:-5956047	None:intergenic
CAACTTACAATTGTCATATC+TGG	0.370044	7.2:-5955924	None:intergenic
ATTGTAAGTTGTCATGTTGA+AGG	0.424855	7.2:+5955936	MS.gene050448:CDS
AACATCTGATGGTAGAAATC+AGG	0.427976	7.2:+5955982	MS.gene050448:CDS
ATGTGGGCCCACTGAGATAC+AGG	0.441245	7.2:+5956186	MS.gene050448:CDS
GGAAGAAGTTCGAAATTGGC+AGG	0.447899	7.2:-5956043	None:intergenic
TGTATCTCAGTGGGCCCACA+TGG	0.456400	7.2:-5956184	None:intergenic
CAAATCTAAGTGAGCTTGAT+GGG	0.474246	7.2:-5956118	None:intergenic
TTCGAACTTCTTCCAAGTGA+AGG	0.478479	7.2:+5956052	MS.gene050448:CDS
TCAAATCTAAGTGAGCTTGA+TGG	0.486532	7.2:-5956119	None:intergenic
GACCAGATCGAAGTTGTAGA+AGG	0.489153	7.2:-5956094	None:intergenic
GGTTGGAACTGAGGATATCA+AGG	0.505962	7.2:+5955875	None:intergenic
GATTGATGATCAGCTGAAGC+AGG	0.507779	7.2:+5956208	MS.gene050448:CDS
AGTTGTCATGTTGAAGGAAA+CGG	0.513703	7.2:+5955942	MS.gene050448:CDS
TCGAACTTCTTCCAAGTGAA+GGG	0.516414	7.2:+5956053	MS.gene050448:CDS
CACCTTCTACAACTTCGATC+TGG	0.522791	7.2:+5956092	MS.gene050448:CDS
TGAAGGGCTTATCGAAGGGC+AGG	0.529646	7.2:+5956069	MS.gene050448:CDS
AAGAAGTTCGAAATTGGCAG+GGG	0.530875	7.2:-5956041	None:intergenic
TGATATCATTGAGGTCGACT+TGG	0.534767	7.2:+5956145	MS.gene050448:CDS
ATTTGAATCTGATATCATTG+AGG	0.563041	7.2:+5956136	MS.gene050448:CDS
CCTTCGATAAGCCCTTCACT+TGG	0.563336	7.2:-5956064	None:intergenic
CCAAGTGAAGGGCTTATCGA+AGG	0.581387	7.2:+5956064	MS.gene050448:CDS
GAAGAAGTTCGAAATTGGCA+GGG	0.582032	7.2:-5956042	None:intergenic
ATGTTTCCAGGTTGGAACTG+AGG	0.588059	7.2:+5955866	None:intergenic
CATCAATCCTGTATCTCAGT+GGG	0.588309	7.2:-5956193	None:intergenic
TGAGCTTCGTAAACATCTGA+TGG	0.589751	7.2:+5955971	MS.gene050448:CDS
TCATGTTGAAGGAAACGGTA+CGG	0.617690	7.2:+5955947	MS.gene050448:CDS
GTATCTCAGTGGGCCCACAT+GGG	0.619772	7.2:-5956183	None:intergenic
CAAGTGAAGGGCTTATCGAA+GGG	0.624792	7.2:+5956065	MS.gene050448:CDS
GTTGGAACTGAGGATATCAA+GGG	0.626948	7.2:+5955876	MS.gene050448:CDS
TCATCAATCCTGTATCTCAG+TGG	0.679879	7.2:-5956194	None:intergenic
AGAAGTTCGAAATTGGCAGG+GGG	0.705475	7.2:-5956040	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTGGATTTTATAGTTTTTG+AGG	+	chr7.2:5955899-5955918	MS.gene050448:CDS	20.0%
!	ATTTGAATCTGATATCATTG+AGG	+	chr7.2:5956136-5956155	MS.gene050448:CDS	25.0%
	AAAAATGTTCAAACCCATGT+GGG	+	chr7.2:5956170-5956189	MS.gene050448:CDS	30.0%
	ATTGTAAGTTGTCATGTTGA+AGG	+	chr7.2:5955936-5955955	MS.gene050448:CDS	30.0%
	CAACTTACAATTGTCATATC+TGG	-	chr7.2:5955927-5955946	None:intergenic	30.0%
	AACATCTGATGGTAGAAATC+AGG	+	chr7.2:5955982-5956001	MS.gene050448:CDS	35.0%
	ACTTGGAAGAAGTTCGAAAT+TGG	-	chr7.2:5956050-5956069	None:intergenic	35.0%
	AGTTGTCATGTTGAAGGAAA+CGG	+	chr7.2:5955942-5955961	MS.gene050448:CDS	35.0%
	CAAAAATGTTCAAACCCATG+TGG	+	chr7.2:5956169-5956188	MS.gene050448:CDS	35.0%
	CAAATCTAAGTGAGCTTGAT+GGG	-	chr7.2:5956121-5956140	None:intergenic	35.0%
	TCAAATCTAAGTGAGCTTGA+TGG	-	chr7.2:5956122-5956141	None:intergenic	35.0%
	AACTGAGGATATCAAGGGTT+TGG	+	chr7.2:5955881-5955900	MS.gene050448:CDS	40.0%
	AAGAAGTTCGAAATTGGCAG+GGG	-	chr7.2:5956044-5956063	None:intergenic	40.0%
	CATCAATCCTGTATCTCAGT+GGG	-	chr7.2:5956196-5956215	None:intergenic	40.0%
	GAAGAAGTTCGAAATTGGCA+GGG	-	chr7.2:5956045-5956064	None:intergenic	40.0%
	GTTGGAACTGAGGATATCAA+GGG	+	chr7.2:5955876-5955895	MS.gene050448:CDS	40.0%
	TCATCAATCCTGTATCTCAG+TGG	-	chr7.2:5956197-5956216	None:intergenic	40.0%
	TCATGTTGAAGGAAACGGTA+CGG	+	chr7.2:5955947-5955966	MS.gene050448:CDS	40.0%
	TCGAACTTCTTCCAAGTGAA+GGG	+	chr7.2:5956053-5956072	MS.gene050448:CDS	40.0%
	TGAGCTTCGTAAACATCTGA+TGG	+	chr7.2:5955971-5955990	MS.gene050448:CDS	40.0%
	TGATATCATTGAGGTCGACT+TGG	+	chr7.2:5956145-5956164	MS.gene050448:CDS	40.0%
	TTCGAACTTCTTCCAAGTGA+AGG	+	chr7.2:5956052-5956071	MS.gene050448:CDS	40.0%
	AGAAGTTCGAAATTGGCAGG+GGG	-	chr7.2:5956043-5956062	None:intergenic	45.0%
	CAAGTGAAGGGCTTATCGAA+GGG	+	chr7.2:5956065-5956084	MS.gene050448:CDS	45.0%
	CACCTTCTACAACTTCGATC+TGG	+	chr7.2:5956092-5956111	MS.gene050448:CDS	45.0%
	GACCAGATCGAAGTTGTAGA+AGG	-	chr7.2:5956097-5956116	None:intergenic	45.0%
	GGAAGAAGTTCGAAATTGGC+AGG	-	chr7.2:5956046-5956065	None:intergenic	45.0%
!	GATTGATGATCAGCTGAAGC+AGG	+	chr7.2:5956208-5956227	MS.gene050448:CDS	45.0%
	CCAAGTGAAGGGCTTATCGA+AGG	+	chr7.2:5956064-5956083	MS.gene050448:CDS	50.0%
	CCTTCGATAAGCCCTTCACT+TGG	-	chr7.2:5956067-5956086	None:intergenic	50.0%
	ATGTGGGCCCACTGAGATAC+AGG	+	chr7.2:5956186-5956205	MS.gene050448:CDS	55.0%
	GTATCTCAGTGGGCCCACAT+GGG	-	chr7.2:5956186-5956205	None:intergenic	55.0%
	TGAAGGGCTTATCGAAGGGC+AGG	+	chr7.2:5956069-5956088	MS.gene050448:CDS	55.0%
	TGTATCTCAGTGGGCCCACA+TGG	-	chr7.2:5956187-5956206	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	5955876	5956229	5955876	ID=MS.gene050448
chr7.2	mRNA	5955876	5956229	5955876	ID=MS.gene050448.t1;Parent=MS.gene050448
chr7.2	exon	5955876	5956229	5955876	ID=MS.gene050448.t1.exon1;Parent=MS.gene050448.t1
chr7.2	CDS	5955876	5956229	5955876	ID=cds.MS.gene050448.t1;Parent=MS.gene050448.t1

Gene Sequence	>MS.gene050448 GTTGGAACTGAGGATATCAAGGGTTTGGATTTTATAGTTTTTGAGGAGCCAGATATGACAATTGTAAGTTGTCATGTTGAAGGAAACGGTACGGATGAGCTTCGTAAACATCTGATGGTAGAAATCAGGTCTGCTAGTGAGACAAGCAAGATAGAGTCAGTCTTCCCCCTGCCAATTTCGAACTTCTTCCAAGTGAAGGGCTTATCGAAGGGCAGGCACCTTCTACAACTTCGATCTGGTCTCCCATCAAGCTCACTTAGATTTGAATCTGATATCATTGAGGTCGACTTGGACAAAAATGTTCAAACCCATGTGGGCCCACTGAGATACAGGATTGATGATCAGCTGAAGCAG
Protein sequence	>MS.gene050448.t1 VGTEDIKGLDFIVFEEPDMTIVSCHVEGNGTDELRKHLMVEIRSASETSKIESVFPLPISNFFQVKGLSKGRHLLQLRSGLPSSSLRFESDIIEVDLDKNVQTHVGPLRYRIDDQLKQ