AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050540.t1 MTR_7g115340 98.643 442 6 0 1 442 69 510 0.0 882
MS.gene050540.t1 MTR_7g115340 98.643 442 6 0 1 442 1 442 0.0 882
MS.gene050540.t1 MTR_1g070380 61.631 417 142 10 38 441 156 567 4.66e-147 431
MS.gene050540.t1 MTR_1g070380 61.722 418 141 11 38 441 156 568 1.13e-145 427
MS.gene050540.t1 MTR_4g127380 41.918 365 180 8 35 388 32 375 2.08e-86 271
MS.gene050540.t1 MTR_2g038020 38.835 412 211 10 12 400 22 415 1.73e-83 264
MS.gene050540.t1 MTR_2g019090 25.641 351 220 8 46 388 311 628 2.17e-25 109
MS.gene050540.t1 MTR_2g019090 24.234 359 204 10 49 375 44 366 1.87e-15 79.0
MS.gene050540.t1 MTR_6g012630 36.842 171 98 4 46 206 46 216 3.52e-25 107
MS.gene050540.t1 MTR_6g012630 36.842 171 98 4 46 206 46 216 4.40e-25 108
MS.gene050540.t1 MTR_6g012630 36.842 171 98 4 46 206 46 216 5.85e-25 107
MS.gene050540.t1 MTR_4g008080 26.546 388 225 14 46 388 226 598 8.63e-23 101
MS.gene050540.t1 MTR_7g011080 32.447 188 110 4 36 206 38 225 3.04e-22 99.8
MS.gene050540.t1 MTR_7g080630 32.544 169 106 2 48 208 101 269 4.72e-22 96.3
MS.gene050540.t1 MTR_7g080630 32.544 169 106 2 48 208 101 269 5.82e-22 96.3
MS.gene050540.t1 MTR_7g080630 32.544 169 106 2 48 208 101 269 1.47e-21 96.3
MS.gene050540.t1 MTR_3g110650 25.281 356 238 9 48 388 313 655 3.29e-21 97.1
MS.gene050540.t1 MTR_4g093650 36.686 169 99 5 45 207 18 184 1.11e-18 89.0
MS.gene050540.t1 MTR_4g093650 24.202 376 233 14 46 393 265 616 1.56e-18 88.6
MS.gene050540.t1 MTR_2g019090 23.955 359 206 9 49 375 44 367 2.90e-16 81.6
MS.gene050540.t1 MTR_2g019090 23.642 313 198 8 46 350 312 591 1.69e-15 79.3
MS.gene050540.t1 MTR_5g031720 28.191 188 119 5 32 207 21 204 1.46e-15 79.3
MS.gene050540.t1 MTR_5g031720 25.138 362 213 11 48 393 276 595 6.32e-14 74.3
MS.gene050540.t1 MTR_2g009650 26.852 216 132 5 36 233 37 244 8.08e-15 77.4
MS.gene050540.t1 MTR_2g009650 24.706 170 123 1 46 210 330 499 1.71e-13 73.2
MS.gene050540.t1 MTR_4g116880 58.904 73 12 3 94 166 10 64 5.31e-14 67.0
MS.gene050540.t1 MTR_3g091800 23.926 163 117 2 48 210 332 487 3.95e-12 68.6
MS.gene050540.t1 MTR_3g091800 27.841 176 111 4 49 208 65 240 4.75e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050540.t1 AT3G04610 67.342 444 116 11 12 436 119 552 0.0 537
MS.gene050540.t1 AT3G04610 67.342 444 116 11 12 436 143 576 0.0 537
MS.gene050540.t1 AT3G04610 67.342 444 116 11 12 436 143 576 0.0 537
MS.gene050540.t1 AT3G04610 67.342 444 116 11 12 436 119 552 0.0 537
MS.gene050540.t1 AT4G26000 42.147 382 193 8 37 408 64 427 2.98e-92 288
MS.gene050540.t1 AT1G51580 26.648 364 224 10 46 388 275 616 7.95e-26 110
MS.gene050540.t1 AT1G51580 27.184 206 107 6 46 208 17 222 5.00e-11 65.1
MS.gene050540.t1 AT5G15270 26.036 338 186 9 46 371 49 334 3.05e-25 108
MS.gene050540.t1 AT5G15270 26.230 183 131 3 32 213 270 449 1.06e-18 89.0
MS.gene050540.t1 AT5G15270 26.036 338 186 9 46 371 49 334 3.05e-25 108
MS.gene050540.t1 AT5G15270 26.230 183 131 3 32 213 270 449 1.06e-18 89.0
MS.gene050540.t1 AT5G15270 25.740 338 187 8 46 371 49 334 3.39e-25 108
MS.gene050540.t1 AT5G15270 26.230 183 131 3 32 213 270 449 1.19e-18 88.6
MS.gene050540.t1 AT1G14170 31.461 178 104 4 46 206 18 194 3.36e-19 90.1
MS.gene050540.t1 AT1G14170 29.379 177 117 4 46 218 243 415 1.18e-11 67.0
MS.gene050540.t1 AT1G14170 31.461 178 104 4 46 206 43 219 3.78e-19 90.1
MS.gene050540.t1 AT1G14170 29.379 177 117 4 46 218 268 440 1.01e-11 67.0
MS.gene050540.t1 AT1G14170 31.461 178 104 4 46 206 10 186 4.01e-19 89.7
MS.gene050540.t1 AT1G14170 29.379 177 117 4 46 218 235 407 1.45e-11 66.6
MS.gene050540.t1 AT4G18375 26.404 356 210 13 48 376 37 367 8.88e-19 89.0
MS.gene050540.t1 AT4G18375 26.404 356 210 13 48 376 37 367 1.48e-18 88.6
MS.gene050540.t1 AT5G64390 24.304 395 241 9 48 388 453 843 2.32e-18 88.6
MS.gene050540.t1 AT5G64390 28.293 205 120 5 45 232 45 239 5.48e-16 80.9
MS.gene050540.t1 AT5G53060 22.543 346 246 5 43 388 321 644 1.22e-16 82.8
MS.gene050540.t1 AT5G53060 26.020 196 121 5 36 208 57 251 4.50e-13 71.6
MS.gene050540.t1 AT5G64390 28.922 204 120 5 45 232 45 239 5.92e-16 80.9
MS.gene050540.t1 AT5G64390 26.012 173 121 2 48 215 453 623 1.01e-13 73.9
MS.gene050540.t1 AT5G64390 28.922 204 120 5 45 232 45 239 6.07e-16 80.9
MS.gene050540.t1 AT5G64390 26.012 173 121 2 48 215 453 623 1.13e-13 73.9
MS.gene050540.t1 AT5G46190 25.926 351 183 14 48 360 45 356 4.43e-14 75.1
MS.gene050540.t1 AT5G46190 25.926 351 183 14 48 360 45 356 4.67e-14 74.7
MS.gene050540.t1 AT5G46190 25.926 351 183 14 48 360 45 356 4.67e-14 74.7
MS.gene050540.t1 AT5G46190 25.926 351 183 14 48 360 45 356 4.67e-14 74.7
MS.gene050540.t1 AT5G53060 26.020 196 121 5 36 208 57 251 3.23e-13 72.0
MS.gene050540.t1 AT5G53060 26.163 172 119 3 43 210 321 488 6.09e-12 68.2

Find 160 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATACAATTTGATGCTTCT+TGG 0.178221 7.4:+2328198 None:intergenic
TCACAAGCAGGAAGTTTAAT+TGG 0.191863 7.4:-2328248 MS.gene050540:CDS
GTAGCCTATGGTGCAGTTTA+TGG 0.255984 7.4:-2324336 MS.gene050540:CDS
TCGTACGCTGATGCTGTTAT+TGG 0.256618 7.4:-2325436 MS.gene050540:CDS
GCAGTTTATGGTGCAAATTA+TGG 0.271012 7.4:-2324324 MS.gene050540:CDS
TGGTGGGGTGGTGGTGGCAC+TGG 0.284770 7.4:+2326088 None:intergenic
CAAAAGGTTGGTGGGATAAT+TGG 0.293067 7.4:-2330782 MS.gene050540:CDS
CACAGCCCGTTCTGAAGTTC+CGG 0.314112 7.4:+2330688 None:intergenic
GATATAGCTTATATTTGTTC+CGG 0.327385 7.4:+2325408 None:intergenic
GCTGGTGGAGGTCCTGGATT+TGG 0.327478 7.4:-2326031 MS.gene050540:CDS
CAGTTTATGGTGCAAATTAT+GGG 0.346952 7.4:-2324323 MS.gene050540:CDS
AACTCCTCTTGTTTCTTGTA+TGG 0.349928 7.4:+2325360 None:intergenic
CCCCATGATTGGTGGGGTGG+TGG 0.363121 7.4:+2326079 None:intergenic
GATGCTCCTTCAGGTGTGGC+TGG 0.367185 7.4:-2328302 MS.gene050540:CDS
GGCACTGGCATGTGCTCCAC+AGG 0.369095 7.4:+2326103 None:intergenic
CAACCCGGCCATTTCTTCTC+AGG 0.371502 7.4:+2330842 None:intergenic
CCAAATGCTGGTGGAGGTCC+TGG 0.372713 7.4:-2326037 MS.gene050540:CDS
CCAATCATGGGGTCCACCTC+AGG 0.379209 7.4:-2326068 MS.gene050540:CDS
CATGATTGGTGGGGTGGTGG+TGG 0.386854 7.4:+2326082 None:intergenic
GCTCGTGTTAAGATTCTTGA+TGG 0.388997 7.4:-2330716 MS.gene050540:CDS
GATGGGTTAGATGCTCCTTC+AGG 0.390825 7.4:-2328311 MS.gene050540:CDS
GACAGGGTGGTTGAAGTAGT+AGG 0.398062 7.4:-2326327 MS.gene050540:CDS
GCTCATAAACGGTTAATTGA+TGG 0.400721 7.4:-2328329 MS.gene050540:CDS
CACTGCCTTGTGCACTCCAA+TGG 0.404296 7.4:+2326299 None:intergenic
AAACTTCCTGCTTGTGAGGC+TGG 0.410456 7.4:+2328254 None:intergenic
CACCACCACCCCACCAATCA+TGG 0.417160 7.4:-2326081 MS.gene050540:CDS
CCACCTGTTTGTTGCTGCGC+CGG 0.419210 7.4:+2324441 None:intergenic
GCACCGGCGCAGCAACAAAC+AGG 0.420300 7.4:-2324444 MS.gene050540:CDS
GAATTGTATCCTTGGTCTGC+AGG 0.425427 7.4:+2324414 None:intergenic
CCAGGACCTCCACCAGCATT+TGG 0.425866 7.4:+2326037 None:intergenic
TCTCTTCCACCTGCTGTGGA+TGG 0.429032 7.4:-2328362 MS.gene050540:CDS
AATGTTGGTTCCTGCTCAAA+AGG 0.429285 7.4:-2330798 MS.gene050540:CDS
TGCTGTGGATGGCTTATTGA+AGG 0.431392 7.4:-2328351 MS.gene050540:CDS
GCCTCCCGGAACTTCAGAAC+GGG 0.433855 7.4:-2330693 MS.gene050540:CDS
TCCTTCAGGTGTGGCTGGGA+AGG 0.436543 7.4:-2328297 MS.gene050540:CDS
TAATTATCAACTTGCCTTGG+AGG 0.437730 7.4:+2325986 None:intergenic
GGGGCTGTTTGTCCAATGGA+GGG 0.440587 7.4:+2325942 None:intergenic
CACAAGCAGGAAGTTTAATT+GGG 0.440852 7.4:-2328247 MS.gene050540:CDS
AAATTGTATAGTCAGAGTTC+TGG 0.448147 7.4:-2328186 MS.gene050540:CDS
CAAGAAACAAGAGGAGTTCC+TGG 0.449581 7.4:-2325355 MS.gene050540:CDS
CAACCACCCTGTCATCTTGA+AGG 0.450885 7.4:+2326337 None:intergenic
GAGGTGGACCCCATGATTGG+TGG 0.454647 7.4:+2326071 None:intergenic
ACAGGGTGGTTGAAGTAGTA+GGG 0.462789 7.4:-2326326 MS.gene050540:CDS
ACTGCCTTGTGCACTCCAAT+GGG 0.463713 7.4:+2326300 None:intergenic
AGGTGGACCCCATGATTGGT+GGG 0.466069 7.4:+2326072 None:intergenic
ACAGCCCGTTCTGAAGTTCC+GGG 0.466480 7.4:+2330689 None:intergenic
AAGATTCTTGATGGGCCTCC+CGG 0.466560 7.4:-2330707 MS.gene050540:CDS
AATTGTATAGTCAGAGTTCT+GGG 0.468142 7.4:-2328185 MS.gene050540:CDS
TCGTTGAGCCTGAGAAGAAA+TGG 0.468519 7.4:-2330850 MS.gene050540:CDS
GATCTTTCTCCAGATGCAAA+TGG 0.469181 7.4:-2326137 MS.gene050540:intron
GGACCCCATGATTGGTGGGG+TGG 0.475178 7.4:+2326076 None:intergenic
TATGGAAGAGATGCTTCAAT+AGG 0.476740 7.4:-2325902 MS.gene050540:CDS
GCTTATTGAAGGCTCATAAA+CGG 0.481382 7.4:-2328340 MS.gene050540:CDS
GGCTGTTTGTCCAATGGAGG+GGG 0.485028 7.4:+2325944 None:intergenic
CTCATAAACGGTTAATTGAT+GGG 0.485762 7.4:-2328328 MS.gene050540:CDS
TTCAATAAGCCATCCACAGC+AGG 0.487643 7.4:+2328353 None:intergenic
GTTCCTGCTCAAAAGGTTGG+TGG 0.488563 7.4:-2330791 MS.gene050540:CDS
ACAGCATCAGCGTACGATAG+AGG 0.489668 7.4:+2325442 None:intergenic
GGCTACAGCATGTCCTGGAT+TGG 0.489729 7.4:+2324353 None:intergenic
CCAGGACATGCTGTAGCCTA+TGG 0.493986 7.4:-2324348 MS.gene050540:CDS
TTCTTCTCTTCCACCTGCTG+TGG 0.494945 7.4:-2328366 MS.gene050540:CDS
TTCTTCTCAGGCTCAACGAT+CGG 0.495988 7.4:+2330854 None:intergenic
CATTGGAGTGCACAAGGCAG+TGG 0.499275 7.4:-2326298 MS.gene050540:CDS
CAGGGTAGGAATTGTATCCT+TGG 0.499993 7.4:+2324406 None:intergenic
AACAGCACAGCAATTGATAC+AGG 0.502245 7.4:-2325290 MS.gene050540:intron
ACCACCACCCCACCAATCAT+GGG 0.502652 7.4:-2326080 MS.gene050540:CDS
CTTGGAGGTGGCATGAATTG+AGG 0.503745 7.4:+2326001 None:intergenic
CCTGAGGTGGACCCCATGAT+TGG 0.504891 7.4:+2326068 None:intergenic
AAGAAATGGCCGGGTTGGCC+TGG 0.507487 7.4:-2330836 MS.gene050540:CDS
GCGTCACCACCTTCCAATCC+AGG 0.508103 7.4:-2324366 MS.gene050540:CDS
CATCATGGTATATCCGCCTA+TGG 0.508306 7.4:-2325920 MS.gene050540:CDS
ATGCTCCTTCAGGTGTGGCT+GGG 0.509317 7.4:-2328301 MS.gene050540:CDS
CAGGGTCTCCCACCAAATGC+TGG 0.510314 7.4:-2326049 MS.gene050540:CDS
CTCCCACCAAATGCTGGTGG+AGG 0.511292 7.4:-2326043 MS.gene050540:CDS
GGGCTGTTTGTCCAATGGAG+GGG 0.511453 7.4:+2325943 None:intergenic
TATGGCGGAAGCTGCAGCAC+CGG 0.517885 7.4:-2324460 MS.gene050540:CDS
TACTCAGTCTGCACCTTCCA+TGG 0.518367 7.4:-2325861 MS.gene050540:CDS
TTATCAACTTGCCTTGGAGG+TGG 0.522949 7.4:+2325989 None:intergenic
CCATAGGCTACAGCATGTCC+TGG 0.523583 7.4:+2324348 None:intergenic
AATTAAACTTCCTGCTTGTG+AGG 0.525068 7.4:+2328250 None:intergenic
TGGGGCTGTTTGTCCAATGG+AGG 0.525515 7.4:+2325941 None:intergenic
CTGAGAAGAAATGGCCGGGT+TGG 0.525773 7.4:-2330841 MS.gene050540:CDS
TAGTAATTATCAACTTGCCT+TGG 0.526071 7.4:+2325983 None:intergenic
GGAACAAAATGAGTCTGAAA+AGG 0.528718 7.4:-2330888 MS.gene050540:CDS
ATTTCAACTGTCATCTCCCC+AGG 0.529708 7.4:+2325337 None:intergenic
AGATGACAGTTGAAATCAGC+GGG 0.541840 7.4:-2325330 MS.gene050540:CDS
CATAGGCGGATATACCATGA+TGG 0.542292 7.4:+2325921 None:intergenic
CCGGCGCAGCAACAAACAGG+TGG 0.553534 7.4:-2324441 MS.gene050540:CDS
ATAGTCAGAGTTCTGGGAGC+AGG 0.562698 7.4:-2328179 MS.gene050540:intron
GCATAGACAGAACTATGAGC+AGG 0.562952 7.4:+2324387 None:intergenic
ACCTTCCCAGCCACACCTGA+AGG 0.563518 7.4:+2328296 None:intergenic
CTCAATTCATGCCACCTCCA+AGG 0.565069 7.4:-2326000 MS.gene050540:CDS
TGTTTCTTGTATGGTAACAG+TGG 0.565082 7.4:+2325369 None:intergenic
TTCCTGCTCAAAAGGTTGGT+GGG 0.565402 7.4:-2330790 MS.gene050540:CDS
GAAAAGTACCTGTGTGACCA+TGG 0.567170 7.4:+2325844 None:intergenic
GGACCTCCACCAGCATTTGG+TGG 0.567350 7.4:+2326040 None:intergenic
TGAGGAGCAAGTCCAAATCC+AGG 0.567540 7.4:+2326019 None:intergenic
ACACTCTCACCAGGCCAACC+CGG 0.568203 7.4:+2330827 None:intergenic
GGCCTCCCGGAACTTCAGAA+CGG 0.568253 7.4:-2330694 MS.gene050540:CDS
TGAGCCTGAGAAGAAATGGC+CGG 0.568727 7.4:-2330846 MS.gene050540:CDS
ATAGGCGGATATACCATGAT+GGG 0.572279 7.4:+2325922 None:intergenic
GAGCCTGAGAAGAAATGGCC+GGG 0.573327 7.4:-2330845 MS.gene050540:CDS
GGAAGGTGCAGACTGAGTAT+TGG 0.573422 7.4:+2325865 None:intergenic
GCACTGGCATGTGCTCCACA+GGG 0.573640 7.4:+2326104 None:intergenic
TATTGAAGCATCTCTTCCAT+AGG 0.574272 7.4:+2325904 None:intergenic
CTCGTGTTAAGATTCTTGAT+GGG 0.574443 7.4:-2330715 MS.gene050540:CDS
ACAGCATGTCCTGGATTGGA+AGG 0.577875 7.4:+2324357 None:intergenic
AAGAAACAAGAGGAGTTCCT+GGG 0.578584 7.4:-2325354 MS.gene050540:CDS
TTGGTTCCTGCTCAAAAGGT+TGG 0.581334 7.4:-2330794 MS.gene050540:CDS
CTAGTGCCAGCCTCACAAGC+AGG 0.582418 7.4:-2328260 MS.gene050540:CDS
GGATCCCATTGGAGTGCACA+AGG 0.586468 7.4:-2326304 MS.gene050540:CDS
TTGGACAAACAGCCCCATCA+TGG 0.587088 7.4:-2325935 MS.gene050540:CDS
GATGCTGTTATTGGAACAGC+CGG 0.587215 7.4:-2325427 MS.gene050540:CDS
AACCACCCTGTCATCTTGAA+GGG 0.588607 7.4:+2326338 None:intergenic
GTATCAGCTAAAGATGAGCC+TGG 0.589213 7.4:-2328389 MS.gene050540:CDS
GTTCTGATCAACCTCAGCCA+TGG 0.592521 7.4:+2330949 None:intergenic
TGCACCATAAACTGCACCAT+AGG 0.596471 7.4:+2324332 None:intergenic
TTGTATCCTTGGTCTGCAGG+AGG 0.600150 7.4:+2324417 None:intergenic
GGTGGACCCCATGATTGGTG+GGG 0.600476 7.4:+2326073 None:intergenic
AGCTATATCAGAAGAGCAAG+TGG 0.600575 7.4:-2325394 MS.gene050540:CDS
TGATGGGGCTGTTTGTCCAA+TGG 0.602005 7.4:+2325938 None:intergenic
GACCTCCACCAGCATTTGGT+GGG 0.602365 7.4:+2326041 None:intergenic
GGTGGGATAATTGGTCGGAA+AGG 0.602653 7.4:-2330773 MS.gene050540:CDS
GACAGAACTATGAGCAGGGT+AGG 0.604594 7.4:+2324392 None:intergenic
CAGTGGAAATTCCATGGCTG+AGG 0.606695 7.4:-2330960 None:intergenic
CAATCATGGGGTCCACCTCA+GGG 0.610026 7.4:-2326067 MS.gene050540:CDS
GCTATATCAGAAGAGCAAGT+GGG 0.610415 7.4:-2325393 MS.gene050540:CDS
ACAACAGCAAATTGTTGAGA+CGG 0.611449 7.4:-2330909 MS.gene050540:CDS
AATAAGCCATCCACAGCAGG+TGG 0.611934 7.4:+2328356 None:intergenic
ACAAAATGAGTCTGAAAAGG+TGG 0.617900 7.4:-2330885 MS.gene050540:CDS
GGTCTCCCACCAAATGCTGG+TGG 0.624009 7.4:-2326046 MS.gene050540:CDS
TGCACCTTCCATGGTCACAC+AGG 0.626823 7.4:-2325852 MS.gene050540:intron
CAGGGTGGTTGAAGTAGTAG+GGG 0.627870 7.4:-2326325 MS.gene050540:CDS
TGAAGCATCTCTTCCATAGG+CGG 0.628282 7.4:+2325907 None:intergenic
ATTCTGAAAACACTCTCACC+AGG 0.628905 7.4:+2330818 None:intergenic
TGCCCTTCAAGATGACAGGG+TGG 0.630655 7.4:-2326340 MS.gene050540:CDS
GAAGTAGTAGGGGATCCCAT+TGG 0.631279 7.4:-2326315 MS.gene050540:CDS
AGTACCTGTGTGACCATGGA+AGG 0.631574 7.4:+2325848 None:intergenic
AGTTTAATTGGGAAACAAGG+AGG 0.632206 7.4:-2328236 MS.gene050540:CDS
GTTACCATACAAGAAACAAG+AGG 0.636417 7.4:-2325364 MS.gene050540:CDS
CTGGCATGTGCTCCACAGGG+TGG 0.637657 7.4:+2326107 None:intergenic
GGAAGTTTAATTGGGAAACA+AGG 0.639460 7.4:-2328239 MS.gene050540:CDS
CTATATCAGAAGAGCAAGTG+GGG 0.641360 7.4:-2325392 MS.gene050540:CDS
GTTAGATGCTCCTTCAGGTG+TGG 0.645046 7.4:-2328306 MS.gene050540:CDS
GCAGGTGGAAGAGAAGAACC+AGG 0.646569 7.4:+2328371 None:intergenic
CGGCGCAGCAACAAACAGGT+GGG 0.647966 7.4:-2324440 MS.gene050540:CDS
AGGTTGGTGGGATAATTGGT+CGG 0.649103 7.4:-2330778 MS.gene050540:CDS
CCACCACCCCACCAATCATG+GGG 0.649451 7.4:-2326079 MS.gene050540:CDS
TTTGGTGGGAGACCCTGAGG+TGG 0.656048 7.4:+2326055 None:intergenic
GGTGGGCCTCCTGCAGACCA+AGG 0.658048 7.4:-2324423 MS.gene050540:CDS
GCATTTGGTGGGAGACCCTG+AGG 0.663331 7.4:+2326052 None:intergenic
CATAGACAGAACTATGAGCA+GGG 0.665116 7.4:+2324388 None:intergenic
TAGGCGGATATACCATGATG+GGG 0.688165 7.4:+2325923 None:intergenic
GCTGAGGTTGATCAGAACGT+AGG 0.692033 7.4:-2330944 MS.gene050540:CDS
TGAGTCTGAAAAGGTGGTTG+AGG 0.692336 7.4:-2330879 MS.gene050540:CDS
GCCCGTTCTGAAGTTCCGGG+AGG 0.698554 7.4:+2330692 None:intergenic
GCATGTCCTGGATTGGAAGG+TGG 0.712815 7.4:+2324360 None:intergenic
AGAAACAAGAGGAGTTCCTG+GGG 0.720239 7.4:-2325353 MS.gene050540:CDS
AATGGCTAATAACCACCCTG+TGG 0.739150 7.4:-2326119 MS.gene050540:CDS
GAGATGACAGTTGAAATCAG+CGG 0.760601 7.4:-2325331 MS.gene050540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.4 gene 2324314 2330969 2324314 ID=MS.gene050540
chr7.4 mRNA 2324314 2330969 2324314 ID=MS.gene050540.t1;Parent=MS.gene050540
chr7.4 exon 2330691 2330969 2330691 ID=MS.gene050540.t1.exon1;Parent=MS.gene050540.t1
chr7.4 CDS 2330691 2330969 2330691 ID=cds.MS.gene050540.t1;Parent=MS.gene050540.t1
chr7.4 exon 2328180 2328417 2328180 ID=MS.gene050540.t1.exon2;Parent=MS.gene050540.t1
chr7.4 CDS 2328180 2328417 2328180 ID=cds.MS.gene050540.t1;Parent=MS.gene050540.t1
chr7.4 exon 2326230 2326378 2326230 ID=MS.gene050540.t1.exon3;Parent=MS.gene050540.t1
chr7.4 CDS 2326230 2326378 2326230 ID=cds.MS.gene050540.t1;Parent=MS.gene050540.t1
chr7.4 exon 2325853 2326146 2325853 ID=MS.gene050540.t1.exon4;Parent=MS.gene050540.t1
chr7.4 CDS 2325853 2326146 2325853 ID=cds.MS.gene050540.t1;Parent=MS.gene050540.t1
chr7.4 exon 2325291 2325485 2325291 ID=MS.gene050540.t1.exon5;Parent=MS.gene050540.t1
chr7.4 CDS 2325291 2325485 2325291 ID=cds.MS.gene050540.t1;Parent=MS.gene050540.t1
chr7.4 exon 2324314 2324487 2324314 ID=MS.gene050540.t1.exon6;Parent=MS.gene050540.t1
chr7.4 CDS 2324314 2324487 2324314 ID=cds.MS.gene050540.t1;Parent=MS.gene050540.t1
Gene Sequence

>MS.gene050540

ATGGCTGAGGTTGATCAGAACGTAGGTGAATTCGAAGAACAACAGCAAATTGTTGAGACGGAACAAAATGAGTCTGAAAAGGTGGTTGAGGTGCCGATCGTTGAGCCTGAGAAGAAATGGCCGGGTTGGCCTGGTGAGAGTGTTTTCAGAATGTTGGTTCCTGCTCAAAAGGTTGGTGGGATAATTGGTCGGAAAGGAGAGTTTATTAAGAAGATTGTTGAAGAGACACGTGCTCGTGTTAAGATTCTTGATGGGCCTCCCGGAACTTCAGAACGGGCTGTGAGTGTTTTCTCTATTCAGCTTACATCTGTTTTTCTGGTTAAAAAGTTAGCTGCATTTGTATCTTGGTAGCATTGATCTGGAATGATTTATTTTTACCAAGCTCTTTTGGATTTTCTTACTTCCTTCATTTTTTAAAAGGATTCATTTGTTTTTGAATATGGTTTTAAAATTTGTGGTTGAGTTGTAGAATAATATTCATGATATCTGTTTTTTATGGAGCTCTGCAGTGATTGTACTCATTACTTGCAATGATGACATAGTTATTTATTTTATCTGTACTTTTACTAATCCTAGATAAGTACATTATAACAAATAATAAACGAAAAATGTATGCACAGATGTGTGTCTGAGCGTCGCATTTTCTAAGACAAGGACCAATGTGAGAAATATTTACGTATGTAATTAGGTATTTACCTATATAATTTGGGTCCAAAATGCTTGTAAATATTCAGAGTAGGAATTATTGGGAGTGAGGGAAAGTACCGTTAATTCTTTTCAAGGACCTGGAATTTGTGTCTGAAACAACTCATAGCAATTATCCATTTAACTATGTCTCTCTCTGCTTAAGATGTTATGATGAAGAATAAATTGTGGCTGTCTTTCCTTAAGCAATCTTAATTTGCAATGTCCTATTCTCACGACAAGAAATTGTAAGAATAAAAAATATAGCTTGTTTATGGTCAGAACAAAGTGAAACCTTATTCATGTGATACAATGAACACAAAAACTAAGATGAAGAAGACGAAACAAATTCGAAAAAGAATTATGGAAAAAATCCACGAAATATGAATTTAATTCTAACAGAAAATGAATCTGTGCAGGTTCAATATTCCATTTTTAAAGGCAACGACTCAATCATCAATTAGATCCCCAATATTGAAGAACCAAAGTTCCAGATGCAGGATCAGCATTGAGCATTGCAAAGTGCTGAAAGTTTATTAACACGTAGTCATTTTATATCAAAGTACCACGAAGCTCTGCATTCCAGCGGGTAAATTCAAAACCTGGATGATTGATGTCTTGCCGCAGATTCCTAGCATTGAAAAAAAGTTTATCACCAGCTCTAATACAATACCGAAAACAATAAATACCAAACCATAAGTATTTTGTTTGTCATACATGTTATCAAGAAAAAAGGATCCGAAGATTAACCAAGGCTATCAATTAATTTTAATTTTAACACATGGAGTATGTCTATGTTAATATTTGTTATGTTATCACAATAGTTCTTCAGTAGGAAATATTGGTAAATTATAGCTATAATTAACTAAATGCACTGTAGTAAACCAAGACCCAATCAATAAAAAAAAATAGAAGACACTTCCACGGTGACATACATTTTAAATCACACGTCATATCACTTATCGTAATTATGTTCACGTATGACACACTTCCATGTTTCTAAAAGGTTTATCTAGGAAATTCCTTTCACGTTTTAATCGCCATTGGATATGAGTAGTAAAGAATGTTTCTTGTGGTAGTTGGAACTTGGTGATATTAAATATACTGGCAAAGGGTTGGGACAAAATGTTGCTTGTTTTCTTCCCTAACAAGAAACAGGTAGAGGGCTGGGGTAAGTTGCACGAGGTGCAGAAGTATTGCATAGATTGGCAGTGGACATAGAAAGGAAAACTGGTAGGGAGCGGGTGGGCGTTCTAGTTAGACAACGAGTTCATGCACACAATCTTCAAAATTATATTTTAGAATTCTCCATGTTTTTTTTTTCTTTTTCTGTTGTCGATAAAAGATGTTTATCAAAATATCTAAACTGTGCTTCAGTTATCTATCTGAAAACCAACATGCTGTTATATTTGGTACTTTACCACAAGCAGTTTGACATTTTAATTTTCAAAGATTGCAATTGGACGAGGATAGGTTAAATAGTAATGTAGAGTATTTGGGTGCAAGAGCTATTCCTCTTAATAATTCGGCCTTTAAGACTGTTACAAAATTGGTTTGGTGGTGAAGTGAAGGATTCCTTCTGGAATTTTTCCGACAGGTCATGATAATGTAGGTATTAATTGTGGAAGAATATTGGCTATTTAGCTGAATCTCCTATTTGTTGCATTTTTAATAATCAAAGTTGCTTGCATTGCATGCTATCTTACTGATCCCGAGATTGTAGGTGGCATGTAAAGGGCTTTGTAATTGGTGCTCTGGTGGCTTTTTATTGTTTGTCACCATATTATGTCTTCGTGTTGCATATTATCATTAGAGGAAATGTTGAGATCAAAGACAGGCTGATAGTGTTATTTGTTCTTTGTGATTAGGTAATGGTATCAGCTAAAGATGAGCCTGGTTCTTCTCTTCCACCTGCTGTGGATGGCTTATTGAAGGCTCATAAACGGTTAATTGATGGGTTAGATGCTCCTTCAGGTGTGGCTGGGAAGGTTTCTACAAAATTTCTAGTGCCAGCCTCACAAGCAGGAAGTTTAATTGGGAAACAAGGAGGCACCGTAAAATCTATCCAAGAAGCATCAAATTGTATAGTCAGAGTTCTGGGAGCAGGTATCTACTCATATTTGATTTAAAAACTTTCAATCTGATTGCTGGATTGTCAATTTTATTATTACTTAGCATTCTGTTATTCTGAGAAGTTCATCGTAGCTTGTATTACAAATTTTCCTTACCCTAATTTTATATCAGAGATAAGTTTGGGATTCATCCACTGGGTACTAGAGCTGTAATAGAGCAATAGTGTAAATATGCTTTTAAGTCTATTAACTGGGATGGTTCCAAGTTCCTCTTCCTATTCGTCAAATGTGTTTTGTTCTTGTCACTGTTTGGTTTATTACCATTTTTACTATTATTGATACTCCTCTCTTTAATTGTCGCTTTTGTAGAAGAATTTTGTTCCTAGAGGTTAATGAGTTTGATCCATATATGGTTTCGGATAGAACATTATGGCGTAATTTTATCCCTGTAGCCGACCCCACTTAGTGGGATAAGGCTTGGTTGTTGTTGTTACCAAGGTTGTTAAACGCACGCTGTAGATCGTGAGATCGTACGATCTCACGCTTTCTTCTCCCTAAACCGTGCCGGCGCGGTGCGAAAATCGTTGAAGAGGGAGGCGCGGCCGGCGCAGAGGTAGATCGCAAGATCGTGGAAGCGGCGAGTTTTCTCACACCGAATTTGTACCTTTTAACCCATATGACCCCGGTTCGCGGAGAAGAGTTCGTTGACCAAAACGACATGCTTTACAGCGGAAGAGAAATGGATGTTGAAGAGTCAAAACAGTTGAGCTTGCCTTTCACGCAGTCAATATTAAAAGCTGATTTATTATTTTTGGAAAGTGTGCCGCTAATTGCTTGTTATTCTTAATTTTATTTTATAATGCTTCTTTTACGGGAATTTTTATTTTTTAATGCTAATTTGCCAAGTTTACTTTTTTAATGCTAGTAATTTGCTAAGTTTACTCTATTTTTAAAAAGATTTCATTGTTTTTAGCCTTAATAAATCTTATATTTAATATATTATTTCATATATTTTAAAAGCTTTATGCTAACAAATACTTAGATAGTTTTTTTTCTAATAAAAATTAATTAAATAGTTAATTCGTTATTTATTTAACATAAGTACTATTTAATTTAATCATATGATTTAATTATATAACACTATATGTAGTATTTTTTTTGTTTGAAGAAACATTCTATACTTAGTAATATTAAGTGTGTTTCTTAATTATAGTATAATCTTGTCTTTGGTGGGATCTTACGATCCTTGTTACGATCTCACGATTTACGATTTTTCTATACCCAAACGATCTTAGATGAGATCTCGATTTTGACAACCATGGTTATTACTGTGATTGTTTTCAAAGCTCAATGAATGAGATAATAAATAATCAAGAATATTAAGGAATAAGACATGATTTCATAAAAAATAATAAAATTTATTGATTGTATTAATATGTGTAAATACCCTTAAAACAACAAGTTAAAAGAAACGGAGGGAGTATCATGTAAGCACTTAGATATGCATAGCTAACTTCTATCATTACCACCTAAACCCTAAACTTACGATAATTCCCGCTATTTATTAATTCAGTTGTCAGATTCATGTATTCTAATCTAAGTAGTTTAAAACTTTCCCTTTCTAAAACCTTTTTTTGTCTATCCTTTTCATTAAAAATTATTTCTAAGATACAATTTGAAGTTTATGCTTCCAGTCTTCTGTAGTTTATGCTTTTAGATAAAATATTAGTGGAAATTTGAAGTTTTTTTCTCTTTCCGTTTTGGTTCTTGGATGGGCTGTTTGACATTCTCTAGTTGCTAAGCATCTTGCACCTCTGTTGTTTTTTTGTCTGCAGAAGATCTGCCTGTTTTTGCCCTTCAAGATGACAGGGTGGTTGAAGTAGTAGGGGATCCCATTGGAGTGCACAAGGCAGTGGAGATGATTGCATCCCACCTTAAGAAATTTTTAGTTGATCGCAGTATAATTCCAATTTTTGAAATGAATGTAAGTGTGTTAATTCATATTACACTTGTGAAGAATCTATCTTATGCTTACCAAGGAATGCAACTAATTTGATCTTTCTCCAGATGCAAATGGCTAATAACCACCCTGTGGAGCACATGCCAGTGCCACCACCACCCCACCAATCATGGGGTCCACCTCAGGGTCTCCCACCAAATGCTGGTGGAGGTCCTGGATTTGGACTTGCTCCTCAATTCATGCCACCTCCAAGGCAAGTTGATAATTACTACCCACCAGCTGAAATGCCCCCTCCATTGGACAAACAGCCCCATCATGGTATATCCGCCTATGGAAGAGATGCTTCAATAGGAGTTCACGCATCATCCAATACTCAGTCTGCACCTTCCATGGTCACACAGGTACTTTTCTTATTTCAAATTTTGTTTTCTAATAATCCCCAAAAGGAAGTAGTATGGTGATTGGGATGATATATTGGCATTACATTTTTATGTAGTTTGCATTTTTCTGGATGTCTTAGAACTCGCCAGTCTGTCTGTCGTTGTTTATGTAGTTTGATGAGACGACTTGTTGACTGCTGCTTCATGAATTTTGTGCAATAGCCGTGGTAAAGGCTAGTTGTGAAACTTTACCAGTGTTGATTGACATTTGCTGTTGCTTTCCACTTTGCTGTTTAGATTTAAGATAGCAGAGGGTGATGTTAGGTTCCACACTTGGATGATAAGCTGAAATGATACTAATTGTCTTGCTAATGCTGTTGTGGCACAGATCACGCAGCAAATGCAAATTCCTCTATCGTACGCTGATGCTGTTATTGGAACAGCCGGAACAAATATAAGCTATATCAGAAGAGCAAGTGGGGCCACTGTTACCATACAAGAAACAAGAGGAGTTCCTGGGGAGATGACAGTTGAAATCAGCGGGACTGCTTCTCAAGTTCAAACAGCACAGCAATTGATACAGGTAATCCTGATCTTGGTCTTTGATTTGCTCCAGCCGTTCTAATATTAGTGTTGTCCTAGGTTTTCTTCAGACTAGGAAAAACTATAAATGAATAAAATAATATTGTAATTTTACAAACTTTTACTCATTTTTTTTTTTGAAGAAGCTAAATTAGCCCACCCTAATTGGCAAACTTTTCCTCATTAACAGTTGGTTATTAACCATTGTTATACTTGGAGGTAGAATTGGAATGAAATAATTAATGCCACTATTAAAAAACAAAACAGTTATTTTAAGACATTTTTATATGTCGAAGCTGCATGCATTTTAGTTTATACATTACAGTTCATAGCATGCGGCATGTCTTGGAAATTTGTTTTAGATTTATACTGAATTAGAGTAATGAAAAAATATTGAATCGTTTACTGGGTTCCTAAAAGATTCATTTTGCGCTAGAGTTTCATCGAAGATTAATTTTTTATGTAAACAGAGAGTAAATTATAAGATTGTTAAATTTCTAAATTAAACTATTATCCTTCCACGAGTATTTTTTCAATAAGTTGACTGGTTGAGTAAGAAATTTGTCATTTTATCCATCCTCACCCCGTCATTTATTTGATGCAAGGAAATTATCTTCAATCTTTTCTGTTATTTCATTTCATGCAGTATCTTTATTTTCTCAATTTGTTTCCATATAAAAGCTATGAATTTACTGTGCATAGTCCTATCTCACCGAATGCATGTCTTGGATGTTAGAACTGATTGACCTAACATTATCAATTTGCCTTGTACTCAAGCAATATAATCCTGAATCTGTGGTGCAGAATTTTATGGCGGAAGCTGCAGCACCGGCGCAGCAACAAACAGGTGGGCCTCCTGCAGACCAAGGATACAATTCCTACCCTGCTCATAGTTCTGTCTATGCGTCACCACCTTCCAATCCAGGACATGCTGTAGCCTATGGTGCAGTTTATGGTGCAAATTATGGGTACCAATGA
Protein sequence

>MS.gene050540.t1

MAEVDQNVGEFEEQQQIVETEQNESEKVVEVPIVEPEKKWPGWPGESVFRMLVPAQKVGGIIGRKGEFIKKIVEETRARVKILDGPPGTSERAVMVSAKDEPGSSLPPAVDGLLKAHKRLIDGLDAPSGVAGKVSTKFLVPASQAGSLIGKQGGTVKSIQEASNCIVRVLGAEDLPVFALQDDRVVEVVGDPIGVHKAVEMIASHLKKFLVDRSIIPIFEMNMQMANNHPVEHMPVPPPPHQSWGPPQGLPPNAGGGPGFGLAPQFMPPPRQVDNYYPPAEMPPPLDKQPHHGISAYGRDASIGVHASSNTQSAPSMVTQITQQMQIPLSYADAVIGTAGTNISYIRRASGATVTIQETRGVPGEMTVEISGTASQVQTAQQLIQNFMAEAAAPAQQQTGGPPADQGYNSYPAHSSVYASPPSNPGHAVAYGAVYGANYGYQ