Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91006.t1 | XP_013443311.1 | 98 | 99 | 2 | 0 | 12 | 110 | 3 | 101 | 1.40E-47 | 198.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91006.t1 | Q9LTK0 | 68.3 | 63 | 20 | 0 | 12 | 74 | 7 | 69 | 4.5e-18 | 92.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91006.t1 | G7ZW11 | 98.0 | 99 | 2 | 0 | 12 | 110 | 3 | 101 | 1.0e-47 | 198.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04915 | MS.gene91006 | 0.817046 | 4.04E-52 | -1.69E-46 |
| MS.gene04917 | MS.gene91006 | 0.815304 | 9.90E-52 | -1.69E-46 |
| MS.gene049378 | MS.gene91006 | 0.815346 | 9.69E-52 | -1.69E-46 |
| MS.gene049438 | MS.gene91006 | 0.804573 | 2.02E-49 | -1.69E-46 |
| MS.gene04991 | MS.gene91006 | -0.857952 | 1.15E-62 | -1.69E-46 |
| MS.gene050114 | MS.gene91006 | 0.80698 | 6.32E-50 | -1.69E-46 |
| MS.gene050540 | MS.gene91006 | -0.802308 | 5.97E-49 | -1.69E-46 |
| MS.gene051122 | MS.gene91006 | 0.824977 | 6.03E-54 | -1.69E-46 |
| MS.gene051184 | MS.gene91006 | 0.841703 | 4.05E-58 | -1.69E-46 |
| MS.gene051541 | MS.gene91006 | 0.841523 | 4.51E-58 | -1.69E-46 |
| MS.gene051655 | MS.gene91006 | 0.857554 | 1.51E-62 | -1.69E-46 |
| MS.gene052493 | MS.gene91006 | 0.802429 | 5.63E-49 | -1.69E-46 |
| MS.gene052586 | MS.gene91006 | 0.80209 | 6.62E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene91006 | 0.829908 | 3.96E-55 | -1.69E-46 |
| MS.gene053340 | MS.gene91006 | 0.803149 | 4.00E-49 | -1.69E-46 |
| MS.gene053726 | MS.gene91006 | 0.80028 | 1.55E-48 | -1.69E-46 |
| MS.gene053963 | MS.gene91006 | 0.805671 | 1.19E-49 | -1.69E-46 |
| MS.gene054222 | MS.gene91006 | 0.804088 | 2.56E-49 | -1.69E-46 |
| MS.gene05439 | MS.gene91006 | 0.806642 | 7.45E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene91006 | 0.813964 | 1.96E-51 | -1.69E-46 |
| MS.gene055442 | MS.gene91006 | 0.809121 | 2.21E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene91006 | 0.807555 | 4.77E-50 | -1.69E-46 |
| MS.gene055864 | MS.gene91006 | 0.800775 | 1.23E-48 | -1.69E-46 |
| MS.gene056638 | MS.gene91006 | 0.823985 | 1.03E-53 | -1.69E-46 |
| MS.gene056849 | MS.gene91006 | 0.806904 | 6.56E-50 | -1.69E-46 |
| MS.gene057155 | MS.gene91006 | 0.8011 | 1.06E-48 | -1.69E-46 |
| MS.gene05761 | MS.gene91006 | 0.830439 | 2.93E-55 | -1.69E-46 |
| MS.gene058081 | MS.gene91006 | 0.800974 | 1.12E-48 | -1.69E-46 |
| MS.gene058223 | MS.gene91006 | -0.806943 | 6.43E-50 | -1.69E-46 |
| MS.gene058429 | MS.gene91006 | 0.845974 | 2.91E-59 | -1.69E-46 |
| MS.gene059872 | MS.gene91006 | -0.800995 | 1.11E-48 | -1.69E-46 |
| MS.gene06010 | MS.gene91006 | 0.828077 | 1.10E-54 | -1.69E-46 |
| MS.gene060520 | MS.gene91006 | 0.83956 | 1.47E-57 | -1.69E-46 |
| MS.gene060850 | MS.gene91006 | 0.826321 | 2.89E-54 | -1.69E-46 |
| MS.gene060851 | MS.gene91006 | 0.843671 | 1.21E-58 | -1.69E-46 |
| MS.gene060852 | MS.gene91006 | 0.84356 | 1.30E-58 | -1.69E-46 |
| MS.gene060869 | MS.gene91006 | 0.845174 | 4.79E-59 | -1.69E-46 |
| MS.gene060870 | MS.gene91006 | 0.821633 | 3.64E-53 | -1.69E-46 |
| MS.gene060879 | MS.gene91006 | 0.806933 | 6.47E-50 | -1.69E-46 |
| MS.gene060932 | MS.gene91006 | 0.802741 | 4.86E-49 | -1.69E-46 |
| MS.gene06121 | MS.gene91006 | 0.804432 | 2.17E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91006.t1 | MTR_0022s0300 | 96.040 | 101 | 4 | 0 | 10 | 110 | 1 | 101 | 1.52e-67 | 201 |
| MS.gene91006.t1 | MTR_1g100747 | 55.000 | 100 | 44 | 1 | 8 | 107 | 13 | 111 | 3.79e-31 | 108 |
| MS.gene91006.t1 | MTR_1g100747 | 56.122 | 98 | 42 | 1 | 10 | 107 | 1 | 97 | 1.19e-30 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91006.t1 | AT5G52200 | 66.154 | 65 | 22 | 0 | 12 | 76 | 7 | 71 | 4.84e-25 | 93.6 |
Find 27 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCACAAGTTGAAGCAATA+TGG | 0.331820 | 6.1:-38656909 | MS.gene91006:CDS |
| ATAGGATGATATGGAGTCTT+AGG | 0.348637 | 6.1:+38657466 | None:intergenic |
| AGAGGTCCTGGACAATCATT+TGG | 0.413756 | 6.1:-38656853 | MS.gene91006:CDS |
| TCATCAATCATAGGATGATA+TGG | 0.442207 | 6.1:+38657457 | None:intergenic |
| ATACAGTCGTCATCAATCAT+AGG | 0.479820 | 6.1:+38657448 | None:intergenic |
| TGAAAATGGAGAATGATCAA+TGG | 0.488046 | 6.1:-38657881 | None:intergenic |
| TGAGGATGAGCTAGAATCAA+TGG | 0.490132 | 6.1:-38656818 | MS.gene91006:CDS |
| GCATCAAAACTTCCTCGTAC+AGG | 0.491468 | 6.1:+38656955 | None:intergenic |
| GTCCTGGACAATCATTTGGT+TGG | 0.507941 | 6.1:-38656849 | MS.gene91006:CDS |
| CATCAAAACTTCCTCGTACA+GGG | 0.511390 | 6.1:+38656956 | None:intergenic |
| TCTAGTAGCAAAAGAGGTCC+TGG | 0.520565 | 6.1:-38656865 | MS.gene91006:CDS |
| CACATTGTAGGGGACGTGTA+AGG | 0.527184 | 6.1:-38657552 | MS.gene91006:intron |
| TGGACTGCTCTGAGTGATGT+AGG | 0.549752 | 6.1:-38656889 | MS.gene91006:CDS |
| GTAGGCTCTAGTAGCAAAAG+AGG | 0.555158 | 6.1:-38656871 | MS.gene91006:CDS |
| GGCTAACATTGGAGAAATTG+AGG | 0.562179 | 6.1:-38657521 | MS.gene91006:CDS |
| GTCCAACCAAATGATTGTCC+AGG | 0.564149 | 6.1:+38656847 | None:intergenic |
| GGGACGTGTAAGGTGGGATG+AGG | 0.575577 | 6.1:-38657542 | MS.gene91006:CDS |
| ATTGTAGGGGACGTGTAAGG+TGG | 0.592991 | 6.1:-38657549 | MS.gene91006:intron |
| ATCAAAACTTCCTCGTACAG+GGG | 0.616697 | 6.1:+38656957 | None:intergenic |
| AGGTGGGATGAGGCTAACAT+TGG | 0.618920 | 6.1:-38657532 | MS.gene91006:CDS |
| ATTTGGTTGGACATCATCTG+AGG | 0.621563 | 6.1:-38656836 | MS.gene91006:CDS |
| TTGTAGGGGACGTGTAAGGT+GGG | 0.629712 | 6.1:-38657548 | MS.gene91006:intron |
| GAATCAATGGAACGAGATGG+TGG | 0.650217 | 6.1:-38656805 | MS.gene91006:CDS |
| CTAGAATCAATGGAACGAGA+TGG | 0.656253 | 6.1:-38656808 | MS.gene91006:CDS |
| TCAATGGAACGAGATGGTGG+TGG | 0.662401 | 6.1:-38656802 | MS.gene91006:CDS |
| GGTTCTCTATCCCCTGTACG+AGG | 0.694449 | 6.1:-38656967 | MS.gene91006:CDS |
| TTGAGGCAAACAAACCTGTG+AGG | 0.736164 | 6.1:-38657504 | MS.gene91006:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACATAATTATAGAATGATA+CGG | + | chr6.1:38656939-38656958 | None:intergenic | 15.0% |
| !! | TATGATATATTACTTAAACA+GGG | - | chr6.1:38657311-38657330 | MS.gene91006:intron | 15.0% |
| !! | AATAAAATGAATCTCACATT+TGG | - | chr6.1:38657080-38657099 | MS.gene91006:intron | 20.0% |
| !! | GTATGATATATTACTTAAAC+AGG | - | chr6.1:38657310-38657329 | MS.gene91006:intron | 20.0% |
| !! | TACTATAGAAACATTCAAAA+TGG | - | chr6.1:38656837-38656856 | MS.gene91006:CDS | 20.0% |
| !!! | CTATTTTTTTTTTTTCCTGA+TGG | + | chr6.1:38657494-38657513 | None:intergenic | 20.0% |
| !!! | TGTTTCTATAGTATAGTATA+TGG | + | chr6.1:38656830-38656849 | None:intergenic | 20.0% |
| ! | ATATATTACTTAAACAGGGA+AGG | - | chr6.1:38657315-38657334 | MS.gene91006:intron | 25.0% |
| !!! | TAGTTTGTCTTTGAAATGTT+TGG | - | chr6.1:38657511-38657530 | MS.gene91006:CDS | 25.0% |
| !!! | TCTGAGATAATATTTTTGTC+TGG | - | chr6.1:38657652-38657671 | MS.gene91006:intron | 25.0% |
| ACATTTGGTATCACATTGTA+GGG | - | chr6.1:38657095-38657114 | MS.gene91006:intron | 30.0% | |
| GACAATGCATAATATATGCA+AGG | - | chr6.1:38657446-38657465 | MS.gene91006:intron | 30.0% | |
| GATAGAGAACCTACAAATAA+GGG | + | chr6.1:38657682-38657701 | None:intergenic | 30.0% | |
| TCATCAATCATAGGATGATA+TGG | + | chr6.1:38657204-38657223 | None:intergenic | 30.0% | |
| TCTGTTAATATAACTGTGTG+AGG | - | chr6.1:38657566-38657585 | MS.gene91006:intron | 30.0% | |
| TTTAGATTCATGCATGAGTA+TGG | - | chr6.1:38657269-38657288 | MS.gene91006:intron | 30.0% | |
| ! | CATGCATGAATCTAAAGTAA+TGG | + | chr6.1:38657265-38657284 | None:intergenic | 30.0% |
| ! | TTATAGAATGATACGGACTA+TGG | + | chr6.1:38656932-38656951 | None:intergenic | 30.0% |
| ATACAGTCGTCATCAATCAT+AGG | + | chr6.1:38657213-38657232 | None:intergenic | 35.0% | |
| ATAGGATGATATGGAGTCTT+AGG | + | chr6.1:38657195-38657214 | None:intergenic | 35.0% | |
| CACATTTGGTATCACATTGT+AGG | - | chr6.1:38657094-38657113 | MS.gene91006:intron | 35.0% | |
| CATTTGGTATCACATTGTAG+GGG | - | chr6.1:38657096-38657115 | MS.gene91006:intron | 35.0% | |
| GGATAGAGAACCTACAAATA+AGG | + | chr6.1:38657683-38657702 | None:intergenic | 35.0% | |
| TGCATAATATATGCAAGGTC+CGG | - | chr6.1:38657451-38657470 | MS.gene91006:intron | 35.0% | |
| ! | CATCACAAGTTGAAGCAATA+TGG | - | chr6.1:38657749-38657768 | MS.gene91006:intron | 35.0% |
| ! | TCTGGATTACCCTTATTTGT+AGG | - | chr6.1:38657670-38657689 | MS.gene91006:intron | 35.0% |
| !!! | GAACTTCTGCTCTTTTTTTG+TGG | - | chr6.1:38656799-38656818 | MS.gene91006:CDS | 35.0% |
| !!! | TTCTGCTCTTTTTTTGTGGT+TGG | - | chr6.1:38656803-38656822 | MS.gene91006:CDS | 35.0% |
| !!! | TTTTTTTTCCTGATGGTGTC+CGG | + | chr6.1:38657487-38657506 | None:intergenic | 35.0% |
| AAAATTCTGCCTCACAGGTT+TGG | + | chr6.1:38656894-38656913 | None:intergenic | 40.0% | |
| AGAAACTACAGTAAGTGAGC+AGG | + | chr6.1:38657605-38657624 | None:intergenic | 40.0% | |
| ATCAAAACTTCCTCGTACAG+GGG | + | chr6.1:38657704-38657723 | None:intergenic | 40.0% | |
| ATTTGGTTGGACATCATCTG+AGG | - | chr6.1:38657822-38657841 | MS.gene91006:intron | 40.0% | |
| CAAACTGACGAGTTAAAAGC+AGG | + | chr6.1:38657055-38657074 | None:intergenic | 40.0% | |
| CATAATATATGCAAGGTCCG+GGG | - | chr6.1:38657453-38657472 | MS.gene91006:CDS | 40.0% | |
| CATCAAAACTTCCTCGTACA+GGG | + | chr6.1:38657705-38657724 | None:intergenic | 40.0% | |
| CTAGAATCAATGGAACGAGA+TGG | - | chr6.1:38657850-38657869 | MS.gene91006:intron | 40.0% | |
| GCATAATATATGCAAGGTCC+GGG | - | chr6.1:38657452-38657471 | MS.gene91006:CDS | 40.0% | |
| GGCTAACATTGGAGAAATTG+AGG | - | chr6.1:38657137-38657156 | MS.gene91006:intron | 40.0% | |
| TGAGGATGAGCTAGAATCAA+TGG | - | chr6.1:38657840-38657859 | MS.gene91006:intron | 40.0% | |
| ! | TCAGTAATTTTCTGCCTCAC+AGG | + | chr6.1:38657171-38657190 | None:intergenic | 40.0% |
| !!! | TTTTTTTCCTGATGGTGTCC+GGG | + | chr6.1:38657486-38657505 | None:intergenic | 40.0% |
| AGAGGTCCTGGACAATCATT+TGG | - | chr6.1:38657805-38657824 | MS.gene91006:intron | 45.0% | |
| GAATCAATGGAACGAGATGG+TGG | - | chr6.1:38657853-38657872 | MS.gene91006:intron | 45.0% | |
| GCATCAAAACTTCCTCGTAC+AGG | + | chr6.1:38657706-38657725 | None:intergenic | 45.0% | |
| GTCCAACCAAATGATTGTCC+AGG | + | chr6.1:38657814-38657833 | None:intergenic | 45.0% | |
| GTCCTGGACAATCATTTGGT+TGG | - | chr6.1:38657809-38657828 | MS.gene91006:intron | 45.0% | |
| TCGGAAAAATTCTGCCTCAC+AGG | + | chr6.1:38656899-38656918 | None:intergenic | 45.0% | |
| TCTAGTAGCAAAAGAGGTCC+TGG | - | chr6.1:38657793-38657812 | MS.gene91006:intron | 45.0% | |
| TTGAGGCAAACAAACCTGTG+AGG | - | chr6.1:38657154-38657173 | MS.gene91006:intron | 45.0% | |
| ! | GTAGGCTCTAGTAGCAAAAG+AGG | - | chr6.1:38657787-38657806 | MS.gene91006:intron | 45.0% |
| ! | TGTGAGGCAGAATTTTTCCG+AGG | - | chr6.1:38656898-38656917 | MS.gene91006:CDS | 45.0% |
| !!! | TTTTTTCCTGATGGTGTCCG+GGG | + | chr6.1:38657485-38657504 | None:intergenic | 45.0% |
| AGGTGGGATGAGGCTAACAT+TGG | - | chr6.1:38657126-38657145 | MS.gene91006:intron | 50.0% | |
| ATTGTAGGGGACGTGTAAGG+TGG | - | chr6.1:38657109-38657128 | MS.gene91006:intron | 50.0% | |
| CACATTGTAGGGGACGTGTA+AGG | - | chr6.1:38657106-38657125 | MS.gene91006:intron | 50.0% | |
| TCTCAGCAACCAAACCTGTG+AGG | - | chr6.1:38656882-38656901 | MS.gene91006:CDS | 50.0% | |
| TTGTAGGGGACGTGTAAGGT+GGG | - | chr6.1:38657110-38657129 | MS.gene91006:intron | 50.0% | |
| ! | TCAATGGAACGAGATGGTGG+TGG | - | chr6.1:38657856-38657875 | MS.gene91006:intron | 50.0% |
| ! | TGGACTGCTCTGAGTGATGT+AGG | - | chr6.1:38657769-38657788 | MS.gene91006:intron | 50.0% |
| CCAACTGAGCTAAGCTCACG+GGG | + | chr6.1:38657422-38657441 | None:intergenic | 55.0% | |
| CGCCAACTGAGCTAAGCTCA+CGG | + | chr6.1:38657424-38657443 | None:intergenic | 55.0% | |
| GCCAACTGAGCTAAGCTCAC+GGG | + | chr6.1:38657423-38657442 | None:intergenic | 55.0% | |
| GGACTATGGACTCTCAGCCT+CGG | + | chr6.1:38656918-38656937 | None:intergenic | 55.0% | |
| GGTTCTCTATCCCCTGTACG+AGG | - | chr6.1:38657691-38657710 | MS.gene91006:intron | 55.0% | |
| TGAGCTTAGCTCAGTTGGCG+AGG | - | chr6.1:38657424-38657443 | MS.gene91006:intron | 55.0% | |
| CCCCGTGAGCTTAGCTCAGT+TGG | - | chr6.1:38657419-38657438 | MS.gene91006:intron | 60.0% | |
| GGGACGTGTAAGGTGGGATG+AGG | - | chr6.1:38657116-38657135 | MS.gene91006:intron | 60.0% | |
| TTCGAACCCCGGACACCATC+AGG | - | chr6.1:38657476-38657495 | MS.gene91006:CDS | 60.0% | |
| AAGGTCCGGGGTTCGAACCC+CGG | - | chr6.1:38657465-38657484 | MS.gene91006:CDS | 65.0% | |
| GGTGTCCGGGGTTCGAACCC+CGG | + | chr6.1:38657473-38657492 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 38656789 | 38657891 | 38656789 | ID=MS.gene91006 |
| chr6.1 | mRNA | 38656789 | 38657891 | 38656789 | ID=MS.gene91006.t1;Parent=MS.gene91006 |
| chr6.1 | exon | 38657860 | 38657891 | 38657860 | ID=MS.gene91006.t1.exon1;Parent=MS.gene91006.t1 |
| chr6.1 | CDS | 38657860 | 38657891 | 38657860 | ID=cds.MS.gene91006.t1;Parent=MS.gene91006.t1 |
| chr6.1 | exon | 38657452 | 38657564 | 38657452 | ID=MS.gene91006.t1.exon2;Parent=MS.gene91006.t1 |
| chr6.1 | CDS | 38657452 | 38657564 | 38657452 | ID=cds.MS.gene91006.t1;Parent=MS.gene91006.t1 |
| chr6.1 | exon | 38656789 | 38656988 | 38656789 | ID=MS.gene91006.t1.exon3;Parent=MS.gene91006.t1 |
| chr6.1 | CDS | 38656789 | 38656988 | 38656789 | ID=cds.MS.gene91006.t1;Parent=MS.gene91006.t1 |
| Gene Sequence |
| Protein sequence |
