AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050661

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050661.t1 MTR_7g117670 94.863 584 15 5 1 579 1 574 0.0 1121
MS.gene050661.t1 MTR_8g027495 58.070 632 178 16 8 572 12 623 0.0 623
MS.gene050661.t1 MTR_7g096350 36.458 576 266 21 10 567 9 502 2.37e-98 308
MS.gene050661.t1 MTR_5g030430 41.256 223 106 5 373 573 458 677 1.42e-41 159
MS.gene050661.t1 MTR_5g030430 27.004 237 154 3 8 240 3 224 9.07e-21 97.1
MS.gene050661.t1 MTR_8g067280 41.872 203 86 5 389 566 444 639 6.34e-40 154
MS.gene050661.t1 MTR_8g067280 31.797 217 115 9 48 253 52 246 1.88e-17 86.3
MS.gene050661.t1 MTR_8g024790 37.791 172 98 1 387 549 271 442 6.55e-33 132
MS.gene050661.t1 MTR_8g024770 37.037 189 105 4 387 562 259 446 2.23e-30 124
MS.gene050661.t1 MTR_5g014600 30.058 173 98 2 383 533 125 296 1.55e-18 87.4
MS.gene050661.t1 MTR_5g030420 29.902 204 124 4 42 241 20 208 2.48e-18 85.1
MS.gene050661.t1 MTR_5g030420 29.902 204 124 4 42 241 20 208 3.33e-18 85.1
MS.gene050661.t1 MTR_4g097950 37.097 124 57 2 407 512 70 190 1.74e-17 82.8
MS.gene050661.t1 MTR_5g014520 26.606 218 144 6 361 565 110 324 1.62e-16 81.6
MS.gene050661.t1 MTR_5g014640 37.302 126 68 4 394 514 145 264 2.64e-16 80.9
MS.gene050661.t1 MTR_4g067010 31.515 165 103 3 394 552 137 297 3.64e-16 80.5
MS.gene050661.t1 MTR_2g010450 29.412 136 87 1 407 533 177 312 5.30e-16 80.1
MS.gene050661.t1 MTR_5g014560 27.957 186 112 4 394 564 119 297 6.51e-16 79.3
MS.gene050661.t1 MTR_4g092700 33.577 137 83 1 405 533 156 292 7.38e-16 79.3
MS.gene050661.t1 MTR_2g104550 33.333 153 74 4 385 509 97 249 7.95e-16 79.0
MS.gene050661.t1 MTR_0011s0210 32.298 161 97 4 400 552 180 336 1.07e-14 76.3
MS.gene050661.t1 MTR_0246s0050 30.233 172 106 4 388 554 136 298 1.19e-14 75.9
MS.gene050661.t1 MTR_4g098035 30.233 172 106 4 388 554 157 319 1.29e-14 75.9
MS.gene050661.t1 MTR_1g072320 22.727 198 131 6 50 241 7 188 1.77e-14 77.0
MS.gene050661.t1 MTR_1g072320 46.512 86 44 2 391 474 453 538 9.40e-14 74.7
MS.gene050661.t1 MTR_0250s0040 32.143 168 98 6 384 542 71 231 2.09e-14 73.9
MS.gene050661.t1 MTR_0246s0020 33.129 163 90 4 393 542 190 346 4.11e-14 74.7
MS.gene050661.t1 MTR_4g066380 49.333 75 34 1 388 462 138 208 4.48e-14 73.9
MS.gene050661.t1 MTR_6g047550 34.532 139 76 4 329 464 104 230 4.79e-14 73.2
MS.gene050661.t1 MTR_0246s0020 33.129 163 90 4 393 542 190 346 4.96e-14 74.3
MS.gene050661.t1 MTR_0011s0260 31.677 161 98 3 400 552 180 336 7.06e-14 73.9
MS.gene050661.t1 MTR_8g098275 52.308 65 31 0 403 467 240 304 8.75e-14 74.3
MS.gene050661.t1 MTR_8g098275 52.308 65 31 0 403 467 304 368 1.17e-13 73.9
MS.gene050661.t1 MTR_3g498695 52.239 67 32 0 405 471 296 362 1.24e-13 73.9
MS.gene050661.t1 MTR_3g498695 52.239 67 32 0 405 471 296 362 1.25e-13 73.9
MS.gene050661.t1 MTR_4g097940 35.897 117 66 3 396 507 148 260 1.26e-13 72.8
MS.gene050661.t1 MTR_8g098275 52.308 65 31 0 403 467 397 461 1.62e-13 73.9
MS.gene050661.t1 MTR_8g098275 26.519 181 112 5 65 239 17 182 1.86e-11 67.4
MS.gene050661.t1 MTR_2g038040 58.182 55 23 0 408 462 53 107 1.76e-13 70.9
MS.gene050661.t1 MTR_1g112680 41.053 95 52 2 370 464 284 374 2.10e-13 73.2
MS.gene050661.t1 MTR_2g084280 30.667 150 98 3 411 554 137 286 2.16e-13 71.6
MS.gene050661.t1 MTR_6g047570 27.273 231 145 7 329 548 90 308 2.35e-13 72.0
MS.gene050661.t1 MTR_4g066460 49.333 75 34 1 388 462 140 210 3.37e-13 71.2
MS.gene050661.t1 MTR_4g009110 26.702 191 106 5 334 509 116 287 4.62e-13 71.2
MS.gene050661.t1 MTR_4g057270 30.000 170 106 5 397 554 140 308 4.70e-13 70.9
MS.gene050661.t1 MTR_7g083900 54.098 61 28 0 405 465 284 344 5.87e-13 71.6
MS.gene050661.t1 MTR_2g104490 33.600 125 68 3 395 509 126 245 6.03e-13 70.1
MS.gene050661.t1 MTR_7g083900 54.098 61 28 0 405 465 284 344 6.21e-13 71.6
MS.gene050661.t1 MTR_2g104490 33.600 125 68 3 395 509 126 245 6.79e-13 70.1
MS.gene050661.t1 MTR_2g022280 26.404 178 108 3 406 561 1 177 1.60e-12 67.0
MS.gene050661.t1 MTR_4g009540 26.573 143 91 2 381 509 126 268 4.98e-12 67.8
MS.gene050661.t1 MTR_4g009540 26.573 143 91 2 381 509 126 268 5.96e-12 67.8
MS.gene050661.t1 MTR_2g104560 24.405 168 120 3 408 568 55 222 1.40e-11 65.1
MS.gene050661.t1 MTR_4g097920 46.154 65 31 1 396 460 148 208 2.07e-11 65.9
MS.gene050661.t1 MTR_4g097920 45.455 66 32 1 395 460 132 193 2.10e-11 65.9
MS.gene050661.t1 MTR_3g030700 43.662 71 40 0 395 465 184 254 2.27e-11 66.2
MS.gene050661.t1 MTR_7g080780 25.000 244 156 7 331 562 79 307 4.20e-11 65.1
MS.gene050661.t1 MTR_7g080780 24.028 283 185 9 295 562 53 320 4.37e-11 65.1
MS.gene050661.t1 MTR_3g104100 48.387 62 29 1 411 472 199 257 6.18e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050661.t1 AT1G01260 47.974 617 248 15 4 578 3 588 3.98e-175 508
MS.gene050661.t1 AT1G01260 47.974 617 248 15 4 578 3 588 3.98e-175 508
MS.gene050661.t1 AT1G01260 47.974 617 248 15 4 578 3 588 3.98e-175 508
MS.gene050661.t1 AT2G46510 47.458 590 240 17 9 573 8 552 2.31e-162 475
MS.gene050661.t1 AT4G17880 33.154 558 298 19 48 566 61 582 2.42e-72 243
MS.gene050661.t1 AT4G16430 50.270 185 73 3 386 567 299 467 6.27e-54 190
MS.gene050661.t1 AT4G16430 37.344 241 120 6 10 241 8 226 1.31e-36 142
MS.gene050661.t1 AT5G46760 47.368 190 87 4 388 565 396 584 7.04e-45 168
MS.gene050661.t1 AT5G46830 30.876 502 269 16 67 549 49 491 9.44e-43 160
MS.gene050661.t1 AT1G32640 43.684 190 91 3 388 565 433 618 2.57e-38 149
MS.gene050661.t1 AT1G32640 32.663 199 117 4 48 241 66 252 9.74e-20 93.6
MS.gene050661.t1 AT5G57150 33.178 214 112 8 387 579 40 243 1.11e-19 89.4
MS.gene050661.t1 AT4G09820 27.039 233 141 7 36 254 2 219 1.41e-18 89.7
MS.gene050661.t1 AT4G09820 34.351 131 55 3 383 509 350 453 6.26e-16 81.3
MS.gene050661.t1 AT4G37850 28.194 227 123 5 359 557 98 312 1.80e-18 87.4
MS.gene050661.t1 AT4G37850 28.194 227 123 5 359 557 98 312 1.80e-18 87.4
MS.gene050661.t1 AT5G57150 33.333 180 97 6 387 549 40 213 2.40e-18 84.7
MS.gene050661.t1 AT5G57150 33.333 180 97 6 387 549 41 214 3.93e-18 84.0
MS.gene050661.t1 AT5G57150 33.333 180 97 6 387 549 41 214 3.94e-18 85.1
MS.gene050661.t1 AT5G57150 33.333 180 97 6 387 549 41 214 4.24e-18 84.0
MS.gene050661.t1 AT4G00870 66.667 57 19 0 406 462 248 304 4.39e-18 87.4
MS.gene050661.t1 AT5G41315 30.332 211 132 4 41 241 1 206 2.04e-16 83.2
MS.gene050661.t1 AT5G41315 48.571 70 36 0 401 470 435 504 9.69e-14 74.7
MS.gene050661.t1 AT5G41315 30.332 211 132 4 41 241 6 211 2.09e-16 83.2
MS.gene050661.t1 AT5G41315 48.571 70 36 0 401 470 440 509 9.18e-14 74.7
MS.gene050661.t1 AT5G41315 30.332 211 132 4 41 241 9 214 2.44e-16 82.8
MS.gene050661.t1 AT5G41315 48.571 70 36 0 401 470 443 512 9.97e-14 74.7
MS.gene050661.t1 AT1G63650 29.224 219 134 7 41 248 1 209 8.25e-16 81.3
MS.gene050661.t1 AT1G63650 48.529 68 35 0 403 470 401 468 1.68e-13 73.9
MS.gene050661.t1 AT1G63650 29.224 219 134 7 41 248 1 209 8.25e-16 81.3
MS.gene050661.t1 AT1G63650 48.529 68 35 0 403 470 401 468 1.68e-13 73.9
MS.gene050661.t1 AT1G63650 29.224 219 134 7 41 248 1 209 8.25e-16 81.3
MS.gene050661.t1 AT1G63650 48.529 68 35 0 403 470 401 468 1.68e-13 73.9
MS.gene050661.t1 AT2G22750 32.911 158 89 3 389 533 140 293 2.34e-14 75.1
MS.gene050661.t1 AT2G22750 32.911 158 89 3 389 533 136 289 2.40e-14 75.1
MS.gene050661.t1 AT2G22750 32.911 158 89 3 389 533 112 265 2.61e-14 74.3
MS.gene050661.t1 AT2G22750 32.911 158 89 3 389 533 140 293 2.61e-14 74.7
MS.gene050661.t1 AT2G22750 32.911 158 89 3 389 533 112 265 2.79e-14 74.3
MS.gene050661.t1 AT1G12860 53.731 67 31 0 405 471 265 331 3.49e-14 75.5
MS.gene050661.t1 AT1G12860 53.731 67 31 0 405 471 265 331 4.01e-14 75.5
MS.gene050661.t1 AT4G29930 31.081 148 95 3 391 537 43 184 4.25e-14 72.4
MS.gene050661.t1 AT4G29930 28.485 165 111 3 391 554 43 201 4.48e-14 72.4
MS.gene050661.t1 AT4G00480 30.319 188 119 6 67 244 36 221 5.34e-14 75.1
MS.gene050661.t1 AT4G00480 30.319 188 119 6 67 244 36 221 7.36e-14 74.7
MS.gene050661.t1 AT4G00480 29.348 184 119 5 67 241 36 217 1.18e-13 74.3
MS.gene050661.t1 AT4G29930 27.545 167 113 4 391 554 43 204 1.97e-13 70.5
MS.gene050661.t1 AT2G16910 55.385 65 27 1 411 475 318 380 1.97e-13 73.6
MS.gene050661.t1 AT3G26744 55.738 61 27 0 405 465 305 365 2.30e-13 73.2
MS.gene050661.t1 AT3G26744 55.738 61 27 0 405 465 305 365 2.45e-13 73.2
MS.gene050661.t1 AT3G26744 55.738 61 27 0 405 465 305 365 2.45e-13 73.2
MS.gene050661.t1 AT3G26744 55.738 61 27 0 405 465 305 365 2.45e-13 73.2
MS.gene050661.t1 AT4G29930 48.611 72 32 1 391 462 43 109 2.47e-13 69.3
MS.gene050661.t1 AT4G29930 48.611 72 32 1 391 462 43 109 3.19e-13 70.5
MS.gene050661.t1 AT4G29930 48.611 72 32 1 391 462 43 109 3.19e-13 70.5
MS.gene050661.t1 AT2G22760 29.851 134 83 2 408 530 120 253 3.25e-13 70.9
MS.gene050661.t1 AT4G29930 48.611 72 32 1 391 462 43 109 3.62e-13 70.1
MS.gene050661.t1 AT2G22760 29.851 134 83 2 408 530 120 253 4.12e-13 70.9
MS.gene050661.t1 AT2G22770 50.000 66 30 1 401 463 123 188 5.02e-12 67.8
MS.gene050661.t1 AT2G28160 45.312 64 35 0 411 474 135 198 5.51e-12 67.4
MS.gene050661.t1 AT2G28160 45.312 64 35 0 411 474 135 198 6.20e-12 67.4
MS.gene050661.t1 AT5G65640 29.747 158 100 3 404 555 175 327 3.10e-11 65.5

Find 108 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAATTCAACACAACTTT+TGG 0.177209 7.3:-805654 None:intergenic
ATTGGAATTACGCAATTTAT+TGG 0.206579 7.3:+805040 MS.gene050661:CDS
CTGACTTAATTACAAAATTA+TGG 0.220746 7.3:-806472 None:intergenic
TTACTGCTTTCGTTCTTTCT+TGG 0.235602 7.3:+805422 MS.gene050661:CDS
TGCGTAATTCCAATTGAAAT+TGG 0.241887 7.3:-805031 None:intergenic
ATGCCTATATGGACAAAGTT+TGG 0.244044 7.3:-806565 None:intergenic
TTCAACAAATCAAAGATTTC+AGG 0.251699 7.3:-805525 None:intergenic
CCGGGTCCACCATACCCTTT+TGG 0.264704 7.3:-805351 None:intergenic
CTCTATTTATCATGCCTATA+TGG 0.268867 7.3:-806576 None:intergenic
CAGAAGTTGCATAAAGCTTT+TGG 0.288502 7.3:+805240 MS.gene050661:CDS
GAAACTTGCCATTAGGAAAA+TGG 0.290252 7.3:+805701 MS.gene050661:CDS
GATGAAGACAATTACGCTTT+TGG 0.306225 7.3:+805270 MS.gene050661:CDS
TCTGCCTCCACATGATTTAG+AGG 0.320999 7.3:-806056 None:intergenic
GATCAATAAAGCGCATGATA+TGG 0.340119 7.3:+804848 MS.gene050661:CDS
TGCCTATATGGACAAAGTTT+GGG 0.350641 7.3:-806564 None:intergenic
ATTCTTCATCCAATTTCAAT+TGG 0.357065 7.3:+805022 MS.gene050661:CDS
ACCAGAAGCAAAACACTTAC+CGG 0.359802 7.3:-805370 None:intergenic
GTTTCGATTACTTTCGAAAC+AGG 0.360473 7.3:-806386 None:intergenic
TTGTCCATCTTGGAAATATT+CGG 0.360963 7.3:-806134 None:intergenic
TTAGGGAGAAACTTGCCATT+AGG 0.364646 7.3:+805694 MS.gene050661:CDS
GGACAAAGTTTGGGTTTGTA+AGG 0.367197 7.3:-806555 None:intergenic
CGACTTTCTAAGTCAAAATC+TGG 0.375807 7.3:+805063 MS.gene050661:CDS
TGTTCTTGGTGCTCGAGCTT+TGG 0.376498 7.3:+804900 MS.gene050661:CDS
ACCATGGTTGGAGCTGTTCT+TGG 0.376666 7.3:+804886 MS.gene050661:CDS
ACTCCACCTTCTTCTTCGTT+TGG 0.377124 7.3:-805126 None:intergenic
CTGGTGATTACGTTCTTTGT+TGG 0.387307 7.3:+805082 MS.gene050661:CDS
TGCATTGGCATCTGCCTTTG+TGG 0.387976 7.3:-805882 None:intergenic
CTAGGTCTCCTTTCATCGCT+AGG 0.390719 7.3:-805996 None:intergenic
CATTTGGAAGCAGTACTTCC+AGG 0.403231 7.3:+806237 MS.gene050661:CDS
ATTCGGTACAACAGCTCTTA+AGG 0.408610 7.3:-806117 None:intergenic
ATGGTATTTGTGGTTCTACT+TGG 0.409907 7.3:+805772 MS.gene050661:CDS
AACTTTCCTAGTGCTAGAAA+TGG 0.411288 7.3:+805753 MS.gene050661:CDS
TCTTTCTTGGCTAAATCTGC+TGG 0.412934 7.3:+805435 MS.gene050661:CDS
CGCGGAAGGAAGCCTGCTAA+TGG 0.414504 7.3:+806023 MS.gene050661:CDS
TGGTGATTACGTTCTTTGTT+GGG 0.415145 7.3:+805083 MS.gene050661:CDS
TAAAGATGCCTTGTCCATCT+TGG 0.422075 7.3:-806144 None:intergenic
TGAAACAAAGTATGAAGAAG+TGG 0.425466 7.3:+805211 MS.gene050661:CDS
TGCTTCTAATGGTGTGAGAA+AGG 0.436502 7.3:+805824 MS.gene050661:CDS
AGTTTGGGTTTGTAAGGAGT+TGG 0.441750 7.3:-806549 None:intergenic
TTGCTTCTGGTAAACATTTG+TGG 0.442625 7.3:+805382 MS.gene050661:CDS
GAATACTAGAGTTTCTCTTC+CGG 0.446637 7.3:+806298 MS.gene050661:CDS
TTGTAAGGAGTTGGATTCTC+GGG 0.450320 7.3:-806540 None:intergenic
GAGTCAGAGAGAGAATCATT+TGG 0.451334 7.3:+806221 MS.gene050661:CDS
ATGGACAAGGCATCTTTACT+TGG 0.451417 7.3:+806149 MS.gene050661:CDS
TAGAGTTTCTCTTCCGGATG+TGG 0.452720 7.3:+806304 MS.gene050661:CDS
GGTTCGTCCCTTCCATTAGC+AGG 0.455729 7.3:-806035 None:intergenic
GGCTTCCTTCCGCGCTTCCT+AGG 0.458293 7.3:-806014 None:intergenic
TGGTATTTGTGGTTCTACTT+GGG 0.461524 7.3:+805773 MS.gene050661:CDS
TACGTTCTTTGTTGGGCAGA+TGG 0.462040 7.3:+805090 MS.gene050661:CDS
AGTGCTAGAAATGGTATTTG+TGG 0.472584 7.3:+805762 MS.gene050661:CDS
TTTGTAAGGAGTTGGATTCT+CGG 0.473242 7.3:-806541 None:intergenic
CTTTCTTCATTGACCACACT+AGG 0.491428 7.3:-805606 None:intergenic
GAAGAAAGTACAGTTTCAAA+TGG 0.494078 7.3:+805621 MS.gene050661:CDS
TGCTGCAATCAACTTGTCCT+TGG 0.495738 7.3:-806513 None:intergenic
AATGATAATGATTGTGTGGC+AGG 0.499914 7.3:-805579 None:intergenic
GAGACCGGTTATTGCTGAAT+CGG 0.502485 7.3:+805944 MS.gene050661:CDS
TCAAGCGAAACTCAAGATCA+TGG 0.503060 7.3:+806199 MS.gene050661:CDS
ACGTTCTTTGTTGGGCAGAT+GGG 0.511903 7.3:+805091 MS.gene050661:CDS
AGTACTTCCAGGGATGGATC+AGG 0.512089 7.3:+806248 MS.gene050661:CDS
TCAAGATGAGGTAATAGTAA+AGG 0.517416 7.3:+806340 MS.gene050661:CDS
AACCCAAACTTTGTCCATAT+AGG 0.517452 7.3:+806562 MS.gene050661:CDS
GGTATTTGTGGTTCTACTTG+GGG 0.519442 7.3:+805774 MS.gene050661:CDS
CGAGTTGCCTGATCCATCCC+TGG 0.522358 7.3:-806255 None:intergenic
CCGGAAAAGTCAATTTGCAT+TGG 0.525652 7.3:-805897 None:intergenic
TTGGCATCTGCCTTTGTGGT+TGG 0.531676 7.3:-805878 None:intergenic
AGTTCCGATTCAGCAATAAC+CGG 0.536707 7.3:-805948 None:intergenic
ATTAGCCCTCGAATTTGCTC+CGG 0.540453 7.3:-805916 None:intergenic
CCAAAAGGGTATGGTGGACC+CGG 0.545307 7.3:+805351 MS.gene050661:CDS
GAGGGCTAATTCTGTGAGAC+CGG 0.547286 7.3:+805929 MS.gene050661:CDS
GGAAGCAGTACTTCCAGGGA+TGG 0.552030 7.3:+806242 MS.gene050661:CDS
TGTACCGAATATTTCCAAGA+TGG 0.552909 7.3:+806130 MS.gene050661:CDS
GAAGCTTGAATATCCACATC+CGG 0.553300 7.3:-806317 None:intergenic
ATGTGGAGGCAGAGAGGCAA+AGG 0.553319 7.3:+806066 MS.gene050661:CDS
CCAGAAGCAAAACACTTACC+GGG 0.554206 7.3:-805369 None:intergenic
GAAGAAGAGAAAACCATGGT+TGG 0.555055 7.3:+804874 MS.gene050661:CDS
GAAACAAAGTATGAAGAAGT+GGG 0.558139 7.3:+805212 MS.gene050661:CDS
GAAGGAAGCCTGCTAATGGA+AGG 0.564326 7.3:+806027 MS.gene050661:CDS
ATTTGGAAGCAGTACTTCCA+GGG 0.565753 7.3:+806238 MS.gene050661:CDS
CTGATCAACCTAGCGATGAA+AGG 0.566880 7.3:+805988 MS.gene050661:CDS
AACTGTTTATCAAATGAGAG+TGG 0.571748 7.3:+804937 MS.gene050661:CDS
TCTGAGTCTTCGCATAGAAG+AGG 0.573258 7.3:+805158 MS.gene050661:CDS
CAAATACCATTTCTAGCACT+AGG 0.582823 7.3:-805759 None:intergenic
GGAGACCTAGGAAGCGCGGA+AGG 0.585153 7.3:+806009 MS.gene050661:CDS
GAGAGTGGATTGATTGCTGT+TGG 0.590794 7.3:+804952 MS.gene050661:CDS
CTGTGTCGGTTACACGGTCA+AGG 0.590950 7.3:-805293 None:intergenic
AGTAATCGAAACATTCAAAG+AGG 0.594015 7.3:+806397 MS.gene050661:CDS
ACATCTCTGTGTCGGTTACA+CGG 0.595335 7.3:-805299 None:intergenic
ACCAAGAACAGCTCCAACCA+TGG 0.601753 7.3:-804887 None:intergenic
AGAGAACCAAACGAAGAAGA+AGG 0.608578 7.3:+805120 MS.gene050661:CDS
ATTATCATTGTATCCTAGTG+TGG 0.610408 7.3:+805593 MS.gene050661:CDS
GGAACTTGTTGCTGATGTTG+AGG 0.613094 7.3:+805965 MS.gene050661:CDS
GAACCAAACGAAGAAGAAGG+TGG 0.613103 7.3:+805123 MS.gene050661:CDS
CTAGCGATGAAAGGAGACCT+AGG 0.617300 7.3:+805997 MS.gene050661:CDS
TATTCAAGCTTCTCAAGATG+AGG 0.619645 7.3:+806328 MS.gene050661:CDS
TCTACTTGGGGAGTGAGTCA+AGG 0.622437 7.3:+805786 MS.gene050661:CDS
TCAACAACACCAAAATCAGT+AGG 0.632462 7.3:-805480 None:intergenic
GTTGTGTTGAATTTGAACAA+TGG 0.637607 7.3:+805660 MS.gene050661:CDS
GGACGAACCTCTAAATCATG+TGG 0.640327 7.3:+806049 MS.gene050661:CDS
AAGGAAGCCTGCTAATGGAA+GGG 0.640978 7.3:+806028 MS.gene050661:CDS
GAAAGGAGACCTAGGAAGCG+CGG 0.657593 7.3:+806005 MS.gene050661:CDS
TGGGGAGTGAGTCAAGGTCA+AGG 0.663327 7.3:+805792 MS.gene050661:CDS
GAATATTTCCAAGATGGACA+AGG 0.676800 7.3:+806136 MS.gene050661:CDS
ATACAATGATAATGATTGTG+TGG 0.679020 7.3:-805583 None:intergenic
TAAATCATGTGGAGGCAGAG+AGG 0.679660 7.3:+806060 MS.gene050661:CDS
CGAACCTCTAAATCATGTGG+AGG 0.682050 7.3:+806052 MS.gene050661:CDS
AGGATCTGAACAGCTTACCA+AGG 0.700634 7.3:+806496 MS.gene050661:CDS
ATAGCAACTACCAACCACAA+AGG 0.712491 7.3:+805868 MS.gene050661:CDS
GAATGAAGAAGAGAAAACCA+TGG 0.720626 7.3:+804870 MS.gene050661:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTGACTTAATTACAAAATTA+TGG - chr7.3:806475-806494 None:intergenic 20.0%
!!! AATTTTGTAATTAAGTCAGA+AGG + chr7.3:806476-806495 MS.gene050661:CDS 20.0%
! ATTCTTCATCCAATTTCAAT+TGG + chr7.3:805022-805041 MS.gene050661:CDS 25.0%
! ATTGGAATTACGCAATTTAT+TGG + chr7.3:805040-805059 MS.gene050661:CDS 25.0%
! TTCAACAAATCAAAGATTTC+AGG - chr7.3:805528-805547 None:intergenic 25.0%
!! ATACAATGATAATGATTGTG+TGG - chr7.3:805586-805605 None:intergenic 25.0%
!! ATTACTATCCATTTTCCTAA+TGG - chr7.3:805712-805731 None:intergenic 25.0%
!! TTCAAATTCAACACAACTTT+TGG - chr7.3:805657-805676 None:intergenic 25.0%
AACTGTTTATCAAATGAGAG+TGG + chr7.3:804937-804956 MS.gene050661:CDS 30.0%
AGTAATCGAAACATTCAAAG+AGG + chr7.3:806397-806416 MS.gene050661:CDS 30.0%
ATTATCATTGTATCCTAGTG+TGG + chr7.3:805593-805612 MS.gene050661:CDS 30.0%
GAAACAAAGTATGAAGAAGT+GGG + chr7.3:805212-805231 MS.gene050661:CDS 30.0%
GTTGTGTTGAATTTGAACAA+TGG + chr7.3:805660-805679 MS.gene050661:CDS 30.0%
TAAAAAGAACATCTCTGTGT+CGG - chr7.3:805310-805329 None:intergenic 30.0%
TCAAGATGAGGTAATAGTAA+AGG + chr7.3:806340-806359 MS.gene050661:CDS 30.0%
TGAAACAAAGTATGAAGAAG+TGG + chr7.3:805211-805230 MS.gene050661:CDS 30.0%
TGCGTAATTCCAATTGAAAT+TGG - chr7.3:805034-805053 None:intergenic 30.0%
TTGTCCATCTTGGAAATATT+CGG - chr7.3:806137-806156 None:intergenic 30.0%
! ATGTACTTCTTTTTCCCAAA+AGG + chr7.3:805336-805355 MS.gene050661:CDS 30.0%
! GAAGAAAGTACAGTTTCAAA+TGG + chr7.3:805621-805640 MS.gene050661:CDS 30.0%
! TGTACTTCTTTTTCCCAAAA+GGG + chr7.3:805337-805356 MS.gene050661:CDS 30.0%
!!! GGTGAATTTTTTGCTTCTAA+TGG + chr7.3:805813-805832 MS.gene050661:CDS 30.0%
!!! GTTTTAGTACCTACTGATTT+TGG + chr7.3:805471-805490 MS.gene050661:CDS 30.0%
AACTTTCCTAGTGCTAGAAA+TGG + chr7.3:805753-805772 MS.gene050661:CDS 35.0%
AGTGCTAGAAATGGTATTTG+TGG + chr7.3:805762-805781 MS.gene050661:CDS 35.0%
CGACTTTCTAAGTCAAAATC+TGG + chr7.3:805063-805082 MS.gene050661:CDS 35.0%
GAAACTTGCCATTAGGAAAA+TGG + chr7.3:805701-805720 MS.gene050661:CDS 35.0%
GAAGAAGAAGAAGAAGAAGA+AGG + chr7.3:805186-805205 MS.gene050661:CDS 35.0%
GAATACTAGAGTTTCTCTTC+CGG + chr7.3:806298-806317 MS.gene050661:CDS 35.0%
GAATATTTCCAAGATGGACA+AGG + chr7.3:806136-806155 MS.gene050661:CDS 35.0%
GAATGAAGAAGAGAAAACCA+TGG + chr7.3:804870-804889 MS.gene050661:CDS 35.0%
GATCAATAAAGCGCATGATA+TGG + chr7.3:804848-804867 MS.gene050661:CDS 35.0%
GTTTCGATTACTTTCGAAAC+AGG - chr7.3:806389-806408 None:intergenic 35.0%
TATTCAAGCTTCTCAAGATG+AGG + chr7.3:806328-806347 MS.gene050661:CDS 35.0%
TCAACAACACCAAAATCAGT+AGG - chr7.3:805483-805502 None:intergenic 35.0%
TGGTGATTACGTTCTTTGTT+GGG + chr7.3:805083-805102 MS.gene050661:CDS 35.0%
TGTACCGAATATTTCCAAGA+TGG + chr7.3:806130-806149 MS.gene050661:CDS 35.0%
TTACTGCTTTCGTTCTTTCT+TGG + chr7.3:805422-805441 MS.gene050661:CDS 35.0%
TTGCTTCTGGTAAACATTTG+TGG + chr7.3:805382-805401 MS.gene050661:CDS 35.0%
TTTGTAAGGAGTTGGATTCT+CGG - chr7.3:806544-806563 None:intergenic 35.0%
! AAGTTGCATAAAGCTTTTGG+TGG + chr7.3:805243-805262 MS.gene050661:CDS 35.0%
! AATGATAATGATTGTGTGGC+AGG - chr7.3:805582-805601 None:intergenic 35.0%
! ACAATGGTCGTACACATTTT+AGG + chr7.3:805676-805695 MS.gene050661:CDS 35.0%
! ATGGTATTTGTGGTTCTACT+TGG + chr7.3:805772-805791 MS.gene050661:CDS 35.0%
! ATGGTGTGAGAAAGGATTTT+AGG + chr7.3:805832-805851 MS.gene050661:CDS 35.0%
! CAATGGTCGTACACATTTTA+GGG + chr7.3:805677-805696 MS.gene050661:CDS 35.0%
! CAGAAGTTGCATAAAGCTTT+TGG + chr7.3:805240-805259 MS.gene050661:CDS 35.0%
! GATGAAGACAATTACGCTTT+TGG + chr7.3:805270-805289 MS.gene050661:CDS 35.0%
! TGGTATTTGTGGTTCTACTT+GGG + chr7.3:805773-805792 MS.gene050661:CDS 35.0%
! TTCTTTTTCCCAAAAGGGTA+TGG + chr7.3:805342-805361 MS.gene050661:CDS 35.0%
!! CAAATACCATTTCTAGCACT+AGG - chr7.3:805762-805781 None:intergenic 35.0%
!! CCAATGCAAATTGACTTTTC+CGG + chr7.3:805897-805916 MS.gene050661:CDS 35.0%
!!! GATTTTGGTGTTGTTGAGTT+GGG + chr7.3:805486-805505 MS.gene050661:CDS 35.0%
!!! TGATTTTGGTGTTGTTGAGT+TGG + chr7.3:805485-805504 MS.gene050661:CDS 35.0%
ACCAGAAGCAAAACACTTAC+CGG - chr7.3:805373-805392 None:intergenic 40.0%
AGAGAACCAAACGAAGAAGA+AGG + chr7.3:805120-805139 MS.gene050661:CDS 40.0%
AGTTCCGATTCAGCAATAAC+CGG - chr7.3:805951-805970 None:intergenic 40.0%
ATAGCAACTACCAACCACAA+AGG + chr7.3:805868-805887 MS.gene050661:CDS 40.0%
ATTCGGTACAACAGCTCTTA+AGG - chr7.3:806120-806139 None:intergenic 40.0%
ATTTGGAAGCAGTACTTCCA+GGG + chr7.3:806238-806257 MS.gene050661:CDS 40.0%
CCGGAAAAGTCAATTTGCAT+TGG - chr7.3:805900-805919 None:intergenic 40.0%
CTGGTGATTACGTTCTTTGT+TGG + chr7.3:805082-805101 MS.gene050661:CDS 40.0%
CTTTCTTCATTGACCACACT+AGG - chr7.3:805609-805628 None:intergenic 40.0%
GAAGAAGAGAAAACCATGGT+TGG + chr7.3:804874-804893 MS.gene050661:CDS 40.0%
GAAGCTTGAATATCCACATC+CGG - chr7.3:806320-806339 None:intergenic 40.0%
GAGTCAGAGAGAGAATCATT+TGG + chr7.3:806221-806240 MS.gene050661:CDS 40.0%
TAAAGATGCCTTGTCCATCT+TGG - chr7.3:806147-806166 None:intergenic 40.0%
TCAAGCGAAACTCAAGATCA+TGG + chr7.3:806199-806218 MS.gene050661:CDS 40.0%
TCTTTCTTGGCTAAATCTGC+TGG + chr7.3:805435-805454 MS.gene050661:CDS 40.0%
TGCTTCTAATGGTGTGAGAA+AGG + chr7.3:805824-805843 MS.gene050661:CDS 40.0%
TTAGGGAGAAACTTGCCATT+AGG + chr7.3:805694-805713 MS.gene050661:CDS 40.0%
TTGTAAGGAGTTGGATTCTC+GGG - chr7.3:806543-806562 None:intergenic 40.0%
! AGTTTGGGTTTGTAAGGAGT+TGG - chr7.3:806552-806571 None:intergenic 40.0%
! ATGGACAAGGCATCTTTACT+TGG + chr7.3:806149-806168 MS.gene050661:CDS 40.0%
! GGACAAAGTTTGGGTTTGTA+AGG - chr7.3:806558-806577 None:intergenic 40.0%
! GGTATTTGTGGTTCTACTTG+GGG + chr7.3:805774-805793 MS.gene050661:CDS 40.0%
!! TTTTTCCCAAAAGGGTATGG+TGG + chr7.3:805345-805364 MS.gene050661:CDS 40.0%
AAGGAAGCCTGCTAATGGAA+GGG + chr7.3:806028-806047 MS.gene050661:CDS 45.0%
ACGTTCTTTGTTGGGCAGAT+GGG + chr7.3:805091-805110 MS.gene050661:CDS 45.0%
ACTCCACCTTCTTCTTCGTT+TGG - chr7.3:805129-805148 None:intergenic 45.0%
AGGATCTGAACAGCTTACCA+AGG + chr7.3:806496-806515 MS.gene050661:CDS 45.0%
CATTTGGAAGCAGTACTTCC+AGG + chr7.3:806237-806256 MS.gene050661:CDS 45.0%
CCAGAAGCAAAACACTTACC+GGG - chr7.3:805372-805391 None:intergenic 45.0%
CGAACCTCTAAATCATGTGG+AGG + chr7.3:806052-806071 MS.gene050661:CDS 45.0%
CTGATCAACCTAGCGATGAA+AGG + chr7.3:805988-806007 MS.gene050661:CDS 45.0%
GAACCAAACGAAGAAGAAGG+TGG + chr7.3:805123-805142 MS.gene050661:CDS 45.0%
GAGACCGGTTATTGCTGAAT+CGG + chr7.3:805944-805963 MS.gene050661:CDS 45.0%
GGACGAACCTCTAAATCATG+TGG + chr7.3:806049-806068 MS.gene050661:CDS 45.0%
TAAATCATGTGGAGGCAGAG+AGG + chr7.3:806060-806079 MS.gene050661:CDS 45.0%
TACGTTCTTTGTTGGGCAGA+TGG + chr7.3:805090-805109 MS.gene050661:CDS 45.0%
TAGAGTTTCTCTTCCGGATG+TGG + chr7.3:806304-806323 MS.gene050661:CDS 45.0%
TCTGAGTCTTCGCATAGAAG+AGG + chr7.3:805158-805177 MS.gene050661:CDS 45.0%
TCTGCCTCCACATGATTTAG+AGG - chr7.3:806059-806078 None:intergenic 45.0%
TGCTGCAATCAACTTGTCCT+TGG - chr7.3:806516-806535 None:intergenic 45.0%
! ACTTTTCCGGAGCAAATTCG+AGG + chr7.3:805910-805929 MS.gene050661:CDS 45.0%
! ATTAGCCCTCGAATTTGCTC+CGG - chr7.3:805919-805938 None:intergenic 45.0%
! CTTTTCCGGAGCAAATTCGA+GGG + chr7.3:805911-805930 MS.gene050661:CDS 45.0%
!! ACATCTCTGTGTCGGTTACA+CGG - chr7.3:805302-805321 None:intergenic 45.0%
!! GAGAGTGGATTGATTGCTGT+TGG + chr7.3:804952-804971 MS.gene050661:CDS 45.0%
!! GGAACTTGTTGCTGATGTTG+AGG + chr7.3:805965-805984 MS.gene050661:CDS 45.0%
ACCAAGAACAGCTCCAACCA+TGG - chr7.3:804890-804909 None:intergenic 50.0%
ACCATGGTTGGAGCTGTTCT+TGG + chr7.3:804886-804905 MS.gene050661:CDS 50.0%
AGTACTTCCAGGGATGGATC+AGG + chr7.3:806248-806267 MS.gene050661:CDS 50.0%
CTAGCGATGAAAGGAGACCT+AGG + chr7.3:805997-806016 MS.gene050661:CDS 50.0%
CTAGGTCTCCTTTCATCGCT+AGG - chr7.3:805999-806018 None:intergenic 50.0%
GAAGGAAGCCTGCTAATGGA+AGG + chr7.3:806027-806046 MS.gene050661:CDS 50.0%
GAGGGCTAATTCTGTGAGAC+CGG + chr7.3:805929-805948 MS.gene050661:CDS 50.0%
TCTACTTGGGGAGTGAGTCA+AGG + chr7.3:805786-805805 MS.gene050661:CDS 50.0%
TTGGCATCTGCCTTTGTGGT+TGG - chr7.3:805881-805900 None:intergenic 50.0%
! CCCGGTAAGTGTTTTGCTTC+TGG + chr7.3:805369-805388 MS.gene050661:CDS 50.0%
! TGCATTGGCATCTGCCTTTG+TGG - chr7.3:805885-805904 None:intergenic 50.0%
!! TGTTCTTGGTGCTCGAGCTT+TGG + chr7.3:804900-804919 MS.gene050661:CDS 50.0%
ATGTGGAGGCAGAGAGGCAA+AGG + chr7.3:806066-806085 MS.gene050661:CDS 55.0%
GAAAGGAGACCTAGGAAGCG+CGG + chr7.3:806005-806024 MS.gene050661:CDS 55.0%
GGAAGCAGTACTTCCAGGGA+TGG + chr7.3:806242-806261 MS.gene050661:CDS 55.0%
GGTTCGTCCCTTCCATTAGC+AGG - chr7.3:806038-806057 None:intergenic 55.0%
TGGGGAGTGAGTCAAGGTCA+AGG + chr7.3:805792-805811 MS.gene050661:CDS 55.0%
! CGGGTCCACCATACCCTTTT+GGG - chr7.3:805353-805372 None:intergenic 55.0%
!! CCAAAAGGGTATGGTGGACC+CGG + chr7.3:805351-805370 MS.gene050661:CDS 55.0%
!! CTGTGTCGGTTACACGGTCA+AGG - chr7.3:805296-805315 None:intergenic 55.0%
CGAGTTGCCTGATCCATCCC+TGG - chr7.3:806258-806277 None:intergenic 60.0%
CGCGGAAGGAAGCCTGCTAA+TGG + chr7.3:806023-806042 MS.gene050661:CDS 60.0%
! CCGGGTCCACCATACCCTTT+TGG - chr7.3:805354-805373 None:intergenic 60.0%
GGCTTCCTTCCGCGCTTCCT+AGG - chr7.3:806017-806036 None:intergenic 65.0%
! GGAGACCTAGGAAGCGCGGA+AGG + chr7.3:806009-806028 MS.gene050661:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 804844 806583 804844 ID=MS.gene050661
chr7.3 mRNA 804844 806583 804844 ID=MS.gene050661.t1;Parent=MS.gene050661
chr7.3 exon 804844 806583 804844 ID=MS.gene050661.t1.exon1;Parent=MS.gene050661.t1
chr7.3 CDS 804844 806583 804844 ID=cds.MS.gene050661.t1;Parent=MS.gene050661.t1
Gene Sequence

>MS.gene050661

ATGGGATCAATAAAGCGCATGATATGGAATGAAGAAGAGAAAACCATGGTTGGAGCTGTTCTTGGTGCTCGAGCTTTGGATTTTTTGATAAATAACTGTTTATCAAATGAGAGTGGATTGATTGCTGTTGGTGAAAGTGTTGATTCTCTTCAAAACAAACTCTCTGATCTTGTTGAAAATTCTTCATCCAATTTCAATTGGAATTACGCAATTTATTGGCGACTTTCTAAGTCAAAATCTGGTGATTACGTTCTTTGTTGGGCAGATGGGTGTTGCAGAGAACCAAACGAAGAAGAAGGTGGAGTTAGAGAAACTCTGAGTCTTCGCATAGAAGAGGAAAAAGAAGAAGAAGAAGAAGAAGAAGGAATGAAACAAAGTATGAAGAAGTGGGTTTTGCAGAAGTTGCATAAAGCTTTTGGTGGTTCAGATGAAGACAATTACGCTTTTGGCCTTGACCGTGTAACCGACACAGAGATGTTCTTTTTAGCTTCTATGTACTTCTTTTTCCCAAAAGGGTATGGTGGACCCGGTAAGTGTTTTGCTTCTGGTAAACATTTGTGGTTCAAATCTGTTTCTGATTACTGCTTTCGTTCTTTCTTGGCTAAATCTGCTGGAATTCAAACTGTTGTTTTAGTACCTACTGATTTTGGTGTTGTTGAGTTGGGTTCTGTTAGATTGTTACCTGAAATCTTTGATTTGTTGAACAAAATCAAATCATTGTTTTCAATTTCAAAACCTGCCACACAATCATTATCATTGTATCCTAGTGTGGTCAATGAAGAAAGTACAGTTTCAAATGGTGTTCAAGTTCCAAAAGTTGTGTTGAATTTGAACAATGGTCGTACACATTTTAGGGAGAAACTTGCCATTAGGAAAATGGATAGTAATAATGTTAATAATAATAGCATTAACTTTCCTAGTGCTAGAAATGGTATTTGTGGTTCTACTTGGGGAGTGAGTCAAGGTCAAGGTGAATTTTTTGCTTCTAATGGTGTGAGAAAGGATTTTAGGCTCAACAACAATAATAGCAACTACCAACCACAAAGGCAGATGCCAATGCAAATTGACTTTTCCGGAGCAAATTCGAGGGCTAATTCTGTGAGACCGGTTATTGCTGAATCGGAACTTGTTGCTGATGTTGAGGCTGATCAACCTAGCGATGAAAGGAGACCTAGGAAGCGCGGAAGGAAGCCTGCTAATGGAAGGGACGAACCTCTAAATCATGTGGAGGCAGAGAGGCAAAGGCGTGAGAAGCTTAATCAGCGTTTTTACGCCTTAAGAGCTGTTGTACCGAATATTTCCAAGATGGACAAGGCATCTTTACTTGGTGATGCTATTGCTTACATCAACGAGCTTCAAGCGAAACTCAAGATCATGGAGTCAGAGAGAGAATCATTTGGAAGCAGTACTTCCAGGGATGGATCAGGCAACTCGAGATCAGAAAATCGTCATCAGAATACTAGAGTTTCTCTTCCGGATGTGGATATTCAAGCTTCTCAAGATGAGGTAATAGTAAAGGTCAGCTGTTCTCTTGATACTCATCCTGTTTCGAAAGTAATCGAAACATTCAAAGAGGCACAAATCAGTGTTGTAGAGTCGAAACTTGCTGCTGCAAATGATACTATCTACCATAATTTTGTAATTAAGTCAGAAGGATCTGAACAGCTTACCAAGGACAAGTTGATTGCAGCATTTGCCCGAGAATCCAACTCCTTACAAACCCAAACTTTGTCCATATAG
Protein sequence

>MS.gene050661.t1

MGSIKRMIWNEEEKTMVGAVLGARALDFLINNCLSNESGLIAVGESVDSLQNKLSDLVENSSSNFNWNYAIYWRLSKSKSGDYVLCWADGCCREPNEEEGGVRETLSLRIEEEKEEEEEEEGMKQSMKKWVLQKLHKAFGGSDEDNYAFGLDRVTDTEMFFLASMYFFFPKGYGGPGKCFASGKHLWFKSVSDYCFRSFLAKSAGIQTVVLVPTDFGVVELGSVRLLPEIFDLLNKIKSLFSISKPATQSLSLYPSVVNEESTVSNGVQVPKVVLNLNNGRTHFREKLAIRKMDSNNVNNNSINFPSARNGICGSTWGVSQGQGEFFASNGVRKDFRLNNNNSNYQPQRQMPMQIDFSGANSRANSVRPVIAESELVADVEADQPSDERRPRKRGRKPANGRDEPLNHVEAERQRREKLNQRFYALRAVVPNISKMDKASLLGDAIAYINELQAKLKIMESERESFGSSTSRDGSGNSRSENRHQNTRVSLPDVDIQASQDEVIVKVSCSLDTHPVSKVIETFKEAQISVVESKLAAANDTIYHNFVIKSEGSEQLTKDKLIAAFARESNSLQTQTLSI