Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050944.t1 | XP_003626174.1 | 73.6 | 129 | 2 | 1 | 1 | 97 | 1 | 129 | 4.70E-44 | 186.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050944.t1 | Q8LG10 | 47.6 | 103 | 22 | 2 | 21 | 91 | 40 | 142 | 4.7e-16 | 85.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050944.t1 | G7KWI0 | 73.6 | 129 | 2 | 1 | 1 | 97 | 1 | 129 | 3.4e-44 | 186.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene050944.t1 | TF | C2C2-GATA |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050944 | MS.gene09850 | 0.803118 | 4.06E-49 | -1.69E-46 |
| MS.gene050944 | MS.gene64517 | 0.800966 | 1.12E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050944.t1 | MTR_7g112330 | 73.643 | 129 | 2 | 1 | 1 | 97 | 1 | 129 | 1.14e-59 | 179 |
| MS.gene050944.t1 | MTR_4g121110 | 39.316 | 117 | 30 | 2 | 21 | 97 | 29 | 144 | 2.07e-20 | 79.7 |
| MS.gene050944.t1 | MTR_4g126350 | 80.000 | 30 | 6 | 0 | 22 | 51 | 186 | 215 | 9.31e-14 | 65.1 |
| MS.gene050944.t1 | MTR_2g038720 | 76.667 | 30 | 7 | 0 | 22 | 51 | 164 | 193 | 1.30e-12 | 62.0 |
| MS.gene050944.t1 | MTR_1g016170 | 68.750 | 32 | 10 | 0 | 22 | 53 | 187 | 218 | 1.86e-12 | 61.6 |
| MS.gene050944.t1 | MTR_2g094600 | 56.410 | 39 | 17 | 0 | 13 | 51 | 207 | 245 | 2.61e-12 | 61.2 |
| MS.gene050944.t1 | MTR_1g116010 | 55.263 | 38 | 16 | 1 | 14 | 51 | 157 | 193 | 8.51e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050944.t1 | AT3G06740 | 46.364 | 110 | 26 | 3 | 21 | 97 | 40 | 149 | 4.19e-23 | 87.0 |
| MS.gene050944.t1 | AT5G49300 | 47.619 | 105 | 27 | 1 | 21 | 97 | 35 | 139 | 8.45e-23 | 85.9 |
| MS.gene050944.t1 | AT5G26930 | 52.632 | 57 | 26 | 1 | 15 | 71 | 20 | 75 | 1.20e-14 | 64.7 |
| MS.gene050944.t1 | AT5G56860 | 75.000 | 36 | 8 | 1 | 18 | 53 | 227 | 261 | 3.31e-14 | 66.6 |
| MS.gene050944.t1 | AT4G26150 | 75.000 | 32 | 8 | 0 | 22 | 53 | 199 | 230 | 5.35e-14 | 66.2 |
| MS.gene050944.t1 | AT3G16870 | 54.545 | 55 | 24 | 1 | 20 | 74 | 40 | 93 | 5.29e-13 | 61.6 |
| MS.gene050944.t1 | AT4G16141 | 41.860 | 86 | 38 | 3 | 21 | 95 | 36 | 120 | 7.39e-13 | 61.6 |
| MS.gene050944.t1 | AT1G08010 | 67.647 | 34 | 10 | 1 | 18 | 51 | 217 | 249 | 1.61e-12 | 61.6 |
| MS.gene050944.t1 | AT1G08010 | 67.647 | 34 | 10 | 1 | 18 | 51 | 217 | 249 | 1.61e-12 | 61.6 |
| MS.gene050944.t1 | AT1G08010 | 67.647 | 34 | 10 | 1 | 18 | 51 | 217 | 249 | 1.61e-12 | 61.6 |
| MS.gene050944.t1 | AT3G50870 | 63.636 | 33 | 12 | 0 | 19 | 51 | 149 | 181 | 1.84e-11 | 58.9 |
| MS.gene050944.t1 | AT2G18380 | 53.191 | 47 | 19 | 1 | 8 | 51 | 75 | 121 | 6.29e-11 | 56.6 |
| MS.gene050944.t1 | AT4G36620 | 60.000 | 35 | 14 | 0 | 17 | 51 | 70 | 104 | 8.24e-11 | 56.2 |
Find 23 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTCTCCAAAGTGGTGTTT+TGG | 0.317787 | 7.1:+3718161 | None:intergenic |
| AATGATACCTTGGGTCCATT+TGG | 0.368205 | 7.1:+3718135 | None:intergenic |
| AATGGGAATCATGGATCAAA+TGG | 0.411033 | 7.1:-3718365 | MS.gene050944:CDS |
| ATGTTGTTCATACGATGAAA+TGG | 0.428480 | 7.1:-3718220 | MS.gene050944:CDS |
| TGTGCAACGCTTGTGGAATC+AGG | 0.447660 | 7.1:-3717856 | MS.gene050944:intron |
| GAAAATGAAGTTAGTGGCAT+TGG | 0.448903 | 7.1:-3717684 | MS.gene050944:CDS |
| AATCATGGATCAAATGGATA+AGG | 0.481862 | 7.1:-3718359 | MS.gene050944:intron |
| AAAATGAAGTTAGTGGCATT+GGG | 0.487144 | 7.1:-3717683 | MS.gene050944:CDS |
| GTGTTTGAAAATGAAGTTAG+TGG | 0.497670 | 7.1:-3717690 | MS.gene050944:CDS |
| TGTTGCAGAAGAAACAAAGA+AGG | 0.501168 | 7.1:-3717649 | MS.gene050944:CDS |
| CTTTGGAGAGGAGGACCAAA+TGG | 0.503109 | 7.1:-3718150 | MS.gene050944:CDS |
| TGTGAATCAAATGGGAATCA+TGG | 0.514991 | 7.1:-3718374 | None:intergenic |
| TGTTTGATGGCACTCTCTTG+TGG | 0.524330 | 7.1:-3717593 | MS.gene050944:CDS |
| AAACCACCAAAACACCACTT+TGG | 0.542523 | 7.1:-3718167 | MS.gene050944:CDS |
| ACAAGCTGCTGTGTGTTTGA+TGG | 0.545240 | 7.1:-3717606 | MS.gene050944:CDS |
| TGTTGTTCATACGATGAAAT+GGG | 0.559954 | 7.1:-3718219 | MS.gene050944:CDS |
| AAGGATTAAACTAGGTGAAG+AGG | 0.572329 | 7.1:-3717630 | MS.gene050944:CDS |
| TAGACGTTGTGCAACGCTTG+TGG | 0.606961 | 7.1:-3717863 | MS.gene050944:CDS |
| AAACAAAGAAGGATTAAACT+AGG | 0.611388 | 7.1:-3717638 | MS.gene050944:CDS |
| TTTGGTCCTCCTCTCCAAAG+TGG | 0.621363 | 7.1:+3718153 | None:intergenic |
| AAAACACCACTTTGGAGAGG+AGG | 0.625709 | 7.1:-3718159 | MS.gene050944:CDS |
| AGGAGGACCAAATGGACCCA+AGG | 0.633971 | 7.1:-3718142 | MS.gene050944:intron |
| ACCAAAACACCACTTTGGAG+AGG | 0.669357 | 7.1:-3718162 | MS.gene050944:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGTTTATTAATTTGATTAA+AGG | - | chr7.1:3717990-3718009 | MS.gene050944:intron | 10.0% |
| !!! | TGTTTATTAATTTGATTAAA+GGG | - | chr7.1:3717991-3718010 | MS.gene050944:intron | 10.0% |
| !! | AAAAAAAAAAAATGATACCT+TGG | + | chr7.1:3717819-3717838 | None:intergenic | 15.0% |
| !! | AAAAAAAAAAATGATACCTT+GGG | + | chr7.1:3717818-3717837 | None:intergenic | 15.0% |
| !! | AAACAATAATCATATCAAAA+AGG | + | chr7.1:3717955-3717974 | None:intergenic | 15.0% |
| !! | AAGTGAATAATATATCAAAT+TGG | + | chr7.1:3717926-3717945 | None:intergenic | 15.0% |
| !!! | GATTTTTTTTTGTTTTTGTT+GGG | - | chr7.1:3718050-3718069 | MS.gene050944:intron | 15.0% |
| !!! | GTTTTCAAATATAGATAAAA+TGG | + | chr7.1:3717898-3717917 | None:intergenic | 15.0% |
| !!! | TGATTTTTTTTTGTTTTTGT+TGG | - | chr7.1:3718049-3718068 | MS.gene050944:intron | 15.0% |
| !! | AAGTTACAAAATGATGAAAT+GGG | + | chr7.1:3717849-3717868 | None:intergenic | 20.0% |
| !! | TAAGTTACAAAATGATGAAA+TGG | + | chr7.1:3717850-3717869 | None:intergenic | 20.0% |
| !! | TGAATAATATATCAAATTGG+TGG | + | chr7.1:3717923-3717942 | None:intergenic | 20.0% |
| ! | AAACAAAGAAGGATTAAACT+AGG | - | chr7.1:3718303-3718322 | MS.gene050944:intron | 25.0% |
| ! | AGATAAAATGGAAGTGAAAA+AGG | + | chr7.1:3717886-3717905 | None:intergenic | 25.0% |
| ! | CTTCTTTCTATTTGTTTAAC+AGG | - | chr7.1:3717697-3717716 | MS.gene050944:intron | 25.0% |
| AAAATGAAGTTAGTGGCATT+GGG | - | chr7.1:3718258-3718277 | MS.gene050944:intron | 30.0% | |
| AATCATGGATCAAATGGATA+AGG | - | chr7.1:3717582-3717601 | MS.gene050944:CDS | 30.0% | |
| ATGTTGTTCATACGATGAAA+TGG | - | chr7.1:3717721-3717740 | MS.gene050944:intron | 30.0% | |
| GTGTTTGAAAATGAAGTTAG+TGG | - | chr7.1:3718251-3718270 | MS.gene050944:intron | 30.0% | |
| TGTTGTTCATACGATGAAAT+GGG | - | chr7.1:3717722-3717741 | MS.gene050944:intron | 30.0% | |
| AAGGATTAAACTAGGTGAAG+AGG | - | chr7.1:3718311-3718330 | MS.gene050944:intron | 35.0% | |
| AATCAGGTACAGAAAGAGAA+GGG | - | chr7.1:3718101-3718120 | MS.gene050944:intron | 35.0% | |
| GAAAATGAAGTTAGTGGCAT+TGG | - | chr7.1:3718257-3718276 | MS.gene050944:intron | 35.0% | |
| TGTTGCAGAAGAAACAAAGA+AGG | - | chr7.1:3718292-3718311 | MS.gene050944:intron | 35.0% | |
| TTGTAGCAAAGGACAAGAAA+GGG | - | chr7.1:3718128-3718147 | MS.gene050944:intron | 35.0% | |
| TTTCTTTGAGCTAACTAGCT+AGG | - | chr7.1:3717619-3717638 | MS.gene050944:CDS | 35.0% | |
| AAACCACCAAAACACCACTT+TGG | - | chr7.1:3717774-3717793 | MS.gene050944:intron | 40.0% | |
| AATGATACCTTGGGTCCATT+TGG | + | chr7.1:3717809-3717828 | None:intergenic | 40.0% | |
| ACAAGAAAGGGAAAGGGAAA+GGG | - | chr7.1:3718140-3718159 | MS.gene050944:intron | 40.0% | |
| ATCAGGTACAGAAAGAGAAG+GGG | - | chr7.1:3718102-3718121 | MS.gene050944:intron | 40.0% | |
| CAAAGGACAAGAAAGGGAAA+GGG | - | chr7.1:3718134-3718153 | MS.gene050944:intron | 40.0% | |
| GAATCAGGTACAGAAAGAGA+AGG | - | chr7.1:3718100-3718119 | MS.gene050944:intron | 40.0% | |
| GTTGTAGCAAAGGACAAGAA+AGG | - | chr7.1:3718127-3718146 | MS.gene050944:intron | 40.0% | |
| AAAGGGAAAGGGAAAGGGAA+AGG | - | chr7.1:3718145-3718164 | MS.gene050944:CDS | 45.0% | |
| AAGGTGCACAGAATGGGAAT+GGG | - | chr7.1:3718178-3718197 | MS.gene050944:CDS | 45.0% | |
| ACAAGCTGCTGTGTGTTTGA+TGG | - | chr7.1:3718335-3718354 | MS.gene050944:intron | 45.0% | |
| AGAAGGGGTTGTTGTAGCAA+AGG | - | chr7.1:3718117-3718136 | MS.gene050944:intron | 45.0% | |
| AGGGAAAGGAAGAGAGAGAA+AGG | - | chr7.1:3718159-3718178 | MS.gene050944:CDS | 45.0% | |
| GACAAGAAAGGGAAAGGGAA+AGG | - | chr7.1:3718139-3718158 | MS.gene050944:intron | 45.0% | |
| GCAAAGGACAAGAAAGGGAA+AGG | - | chr7.1:3718133-3718152 | MS.gene050944:intron | 45.0% | |
| ! | AAAACACCACTTTGGAGAGG+AGG | - | chr7.1:3717782-3717801 | MS.gene050944:intron | 45.0% |
| ! | AAAGGTGCACAGAATGGGAA+TGG | - | chr7.1:3718177-3718196 | MS.gene050944:CDS | 45.0% |
| ! | ACCAAAACACCACTTTGGAG+AGG | - | chr7.1:3717779-3717798 | MS.gene050944:intron | 45.0% |
| ! | AGAGAGAAAGGTGCACAGAA+TGG | - | chr7.1:3718171-3718190 | MS.gene050944:CDS | 45.0% |
| ! | GAGAGAAAGGTGCACAGAAT+GGG | - | chr7.1:3718172-3718191 | MS.gene050944:CDS | 45.0% |
| ! | TCCTCTCCAAAGTGGTGTTT+TGG | + | chr7.1:3717783-3717802 | None:intergenic | 45.0% |
| ! | TGTTTGATGGCACTCTCTTG+TGG | - | chr7.1:3718348-3718367 | MS.gene050944:intron | 45.0% |
| !! | TCTCCAAAGTGGTGTTTTGG+TGG | + | chr7.1:3717780-3717799 | None:intergenic | 45.0% |
| TAGACGTTGTGCAACGCTTG+TGG | - | chr7.1:3718078-3718097 | MS.gene050944:intron | 50.0% | |
| TGCACAGAATGGGAATGGGA+GGG | - | chr7.1:3718182-3718201 | MS.gene050944:CDS | 50.0% | |
| TGTGCAACGCTTGTGGAATC+AGG | - | chr7.1:3718085-3718104 | MS.gene050944:intron | 50.0% | |
| TTTGGTCCTCCTCTCCAAAG+TGG | + | chr7.1:3717791-3717810 | None:intergenic | 50.0% | |
| ! | CTTTGGAGAGGAGGACCAAA+TGG | - | chr7.1:3717791-3717810 | MS.gene050944:intron | 50.0% |
| AGGAGGACCAAATGGACCCA+AGG | - | chr7.1:3717799-3717818 | MS.gene050944:intron | 55.0% | |
| GCACAGAATGGGAATGGGAG+GGG | - | chr7.1:3718183-3718202 | MS.gene050944:CDS | 55.0% | |
| GGTGGTACTAGCAGTAGCAG+TGG | - | chr7.1:3718204-3718223 | MS.gene050944:CDS | 55.0% | |
| GTGCACAGAATGGGAATGGG+AGG | - | chr7.1:3718181-3718200 | MS.gene050944:CDS | 55.0% | |
| CAGAATGGGAATGGGAGGGG+TGG | - | chr7.1:3718186-3718205 | MS.gene050944:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 3717577 | 3718386 | 3717577 | ID=MS.gene050944 |
| chr7.1 | mRNA | 3717577 | 3718386 | 3717577 | ID=MS.gene050944.t1;Parent=MS.gene050944 |
| chr7.1 | exon | 3718360 | 3718386 | 3718360 | ID=MS.gene050944.t1.exon1;Parent=MS.gene050944.t1 |
| chr7.1 | CDS | 3718360 | 3718386 | 3718360 | ID=cds.MS.gene050944.t1;Parent=MS.gene050944.t1 |
| chr7.1 | exon | 3718143 | 3718244 | 3718143 | ID=MS.gene050944.t1.exon2;Parent=MS.gene050944.t1 |
| chr7.1 | CDS | 3718143 | 3718244 | 3718143 | ID=cds.MS.gene050944.t1;Parent=MS.gene050944.t1 |
| chr7.1 | exon | 3717857 | 3717882 | 3717857 | ID=MS.gene050944.t1.exon3;Parent=MS.gene050944.t1 |
| chr7.1 | CDS | 3717857 | 3717882 | 3717857 | ID=cds.MS.gene050944.t1;Parent=MS.gene050944.t1 |
| chr7.1 | exon | 3717577 | 3717715 | 3717577 | ID=MS.gene050944.t1.exon4;Parent=MS.gene050944.t1 |
| chr7.1 | CDS | 3717577 | 3717715 | 3717577 | ID=cds.MS.gene050944.t1;Parent=MS.gene050944.t1 |
| Gene Sequence |
| Protein sequence |
