Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09850.t1 | XP_024625861.1 | 92.4 | 251 | 18 | 1 | 1 | 250 | 1 | 251 | 1.70E-101 | 379 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09850.t1 | G7L0L2 | 92.4 | 251 | 18 | 1 | 1 | 250 | 85 | 335 | 1.2e-101 | 379.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049580 | MS.gene09850 | 0.833168 | 6.23E-56 | -1.69E-46 |
| MS.gene049653 | MS.gene09850 | 0.811914 | 5.52E-51 | -1.69E-46 |
| MS.gene049825 | MS.gene09850 | 0.845512 | 3.88E-59 | -1.69E-46 |
| MS.gene049873 | MS.gene09850 | 0.840369 | 9.06E-58 | -1.69E-46 |
| MS.gene050277 | MS.gene09850 | 0.818994 | 1.47E-52 | -1.69E-46 |
| MS.gene050303 | MS.gene09850 | 0.811768 | 5.94E-51 | -1.69E-46 |
| MS.gene050944 | MS.gene09850 | 0.803118 | 4.06E-49 | -1.69E-46 |
| MS.gene051678 | MS.gene09850 | 0.833274 | 5.86E-56 | -1.69E-46 |
| MS.gene051903 | MS.gene09850 | 0.801242 | 9.88E-49 | -1.69E-46 |
| MS.gene052286 | MS.gene09850 | 0.814711 | 1.34E-51 | -1.69E-46 |
| MS.gene052302 | MS.gene09850 | 0.807634 | 4.59E-50 | -1.69E-46 |
| MS.gene052358 | MS.gene09850 | -0.851903 | 6.55E-61 | -1.69E-46 |
| MS.gene052376 | MS.gene09850 | 0.800759 | 1.24E-48 | -1.69E-46 |
| MS.gene052458 | MS.gene09850 | 0.808749 | 2.66E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene09850 | 0.805713 | 1.17E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene09850 | 0.800924 | 1.15E-48 | -1.69E-46 |
| MS.gene053006 | MS.gene09850 | 0.833563 | 4.96E-56 | -1.69E-46 |
| MS.gene053045 | MS.gene09850 | 0.806267 | 8.94E-50 | -1.69E-46 |
| MS.gene053189 | MS.gene09850 | 0.819026 | 1.44E-52 | -1.69E-46 |
| MS.gene053294 | MS.gene09850 | 0.857777 | 1.30E-62 | -1.69E-46 |
| MS.gene053401 | MS.gene09850 | 0.852506 | 4.42E-61 | -1.69E-46 |
| MS.gene053963 | MS.gene09850 | 0.804974 | 1.67E-49 | -1.69E-46 |
| MS.gene053995 | MS.gene09850 | 0.833297 | 5.78E-56 | -1.69E-46 |
| MS.gene055374 | MS.gene09850 | 0.851867 | 6.71E-61 | -1.69E-46 |
| MS.gene05548 | MS.gene09850 | 0.866524 | 2.68E-65 | -1.69E-46 |
| MS.gene055555 | MS.gene09850 | 0.842938 | 1.90E-58 | -1.69E-46 |
| MS.gene055808 | MS.gene09850 | 0.837385 | 5.36E-57 | -1.69E-46 |
| MS.gene055818 | MS.gene09850 | 0.801021 | 1.10E-48 | -1.69E-46 |
| MS.gene056091 | MS.gene09850 | 0.861611 | 9.08E-64 | -1.69E-46 |
| MS.gene056631 | MS.gene09850 | 0.813498 | 2.48E-51 | -1.69E-46 |
| MS.gene057060 | MS.gene09850 | 0.816641 | 4.98E-52 | -1.69E-46 |
| MS.gene057079 | MS.gene09850 | 0.832496 | 9.14E-56 | -1.69E-46 |
| MS.gene057555 | MS.gene09850 | 0.807171 | 5.76E-50 | -1.69E-46 |
| MS.gene057705 | MS.gene09850 | 0.853099 | 2.99E-61 | -1.69E-46 |
| MS.gene05795 | MS.gene09850 | 0.837882 | 4.00E-57 | -1.69E-46 |
| MS.gene058008 | MS.gene09850 | 0.801948 | 7.08E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene09850 | 0.81628 | 6.00E-52 | -1.69E-46 |
| MS.gene058635 | MS.gene09850 | 0.83297 | 6.97E-56 | -1.69E-46 |
| MS.gene05954 | MS.gene09850 | 0.841776 | 3.87E-58 | -1.69E-46 |
| MS.gene06001 | MS.gene09850 | 0.806798 | 6.91E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene09850 | 0.808325 | 3.27E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene09850 | 0.819858 | 9.32E-53 | -1.69E-46 |
| MS.gene060917 | MS.gene09850 | 0.82057 | 6.40E-53 | -1.69E-46 |
| MS.gene061040 | MS.gene09850 | -0.800112 | 1.68E-48 | -1.69E-46 |
| MS.gene061266 | MS.gene09850 | 0.829709 | 4.42E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene061900 | MS.gene09850 | PPI |
| MS.gene09850 | MS.gene061900 | PPI |
| MS.gene09850 | MS.gene64517 | PPI |
| MS.gene09850 | MS.gene068527 | PPI |
| MS.gene09850 | MS.gene058978 | PPI |
| MS.gene09850 | MS.gene018558 | PPI |
| MS.gene09850 | MS.gene073852 | PPI |
| MS.gene09850 | MS.gene53776 | PPI |
| MS.gene09850 | MS.gene24068 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09850.t1 | MTR_7g099950 | 92.430 | 251 | 18 | 1 | 1 | 250 | 85 | 335 | 9.03e-139 | 393 |
| MS.gene09850.t1 | MTR_1g068825 | 45.312 | 256 | 122 | 5 | 1 | 250 | 1 | 244 | 1.22e-47 | 158 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09850.t1 | AT2G37480 | 40.336 | 119 | 48 | 4 | 80 | 193 | 71 | 171 | 1.71e-13 | 67.4 |
| MS.gene09850.t1 | AT3G53670 | 36.800 | 125 | 71 | 3 | 68 | 192 | 61 | 177 | 1.96e-13 | 67.4 |
| MS.gene09850.t1 | AT3G53670 | 36.800 | 125 | 71 | 3 | 68 | 192 | 61 | 177 | 1.96e-13 | 67.4 |
| MS.gene09850.t1 | AT2G37480 | 40.336 | 119 | 49 | 3 | 80 | 193 | 71 | 172 | 4.01e-13 | 66.6 |
Find 38 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTCCGCAGTTTGAATCT+TGG | 0.342574 | 7.1:+7303893 | None:intergenic |
| GGCAACGTCGCATGAGGAAT+TGG | 0.368193 | 7.1:+7304685 | None:intergenic |
| ATAGGAAATTGACGGTGAAA+TGG | 0.412052 | 7.1:-7304173 | MS.gene09850:CDS |
| TTTATCACGGCTACCTTTCA+AGG | 0.425025 | 7.1:+7304209 | None:intergenic |
| ACAAGAAACAGCCGAAATCC+AGG | 0.447680 | 7.1:-7304098 | MS.gene09850:CDS |
| TAGGAAATTGACGGTGAAAT+GGG | 0.449608 | 7.1:-7304172 | MS.gene09850:CDS |
| TGGGATTCTCAAGTTATTGA+AGG | 0.461070 | 7.1:-7303949 | MS.gene09850:CDS |
| AAGCAGTCTCGCAACGTTGG+TGG | 0.501681 | 7.1:-7303997 | MS.gene09850:CDS |
| TCCTCGTCAGAAGAGCTTGA+AGG | 0.508539 | 7.1:+7304664 | None:intergenic |
| AAGGCAGATTTGAGCTCCGA+AGG | 0.526871 | 7.1:+7304228 | None:intergenic |
| AGCAGTCTCGCAACGTTGGT+GGG | 0.540201 | 7.1:-7303996 | MS.gene09850:CDS |
| GCCTTCAAGCTCTTCTGACG+AGG | 0.541579 | 7.1:-7304665 | MS.gene09850:CDS |
| TGCGACACTAGTGTAGGTAT+AGG | 0.544332 | 7.1:+7304132 | None:intergenic |
| TTCACCGTCAATTTCCTATG+TGG | 0.544822 | 7.1:+7304177 | None:intergenic |
| AAATGTAAAGAAAGGTAACA+AGG | 0.560400 | 7.1:-7304055 | MS.gene09850:CDS |
| AATGTAAAGAAAGGTAACAA+GGG | 0.568123 | 7.1:-7304054 | MS.gene09850:CDS |
| GAGCTCAAATCTGCCTTGAA+AGG | 0.568787 | 7.1:-7304222 | MS.gene09850:CDS |
| TGAACCACATAGGAAATTGA+CGG | 0.581672 | 7.1:-7304181 | MS.gene09850:CDS |
| CTGATCTGTGCAGTAAGTCG+TGG | 0.587620 | 7.1:-7303969 | MS.gene09850:CDS |
| TGCGATGTGCAATGTAGCCA+AGG | 0.589787 | 7.1:-7304782 | MS.gene09850:CDS |
| CTTGAAGGCAACGTCGCATG+AGG | 0.604027 | 7.1:+7304679 | None:intergenic |
| AGAAGTGCATTACTCAGTTG+CGG | 0.604827 | 7.1:-7303848 | MS.gene09850:CDS |
| TGATCTGTGCAGTAAGTCGT+GGG | 0.608135 | 7.1:-7303968 | MS.gene09850:CDS |
| AAGAAGCAGTCTCGCAACGT+TGG | 0.609694 | 7.1:-7304000 | MS.gene09850:CDS |
| TAGCCAAGGAAAACTCAAGT+GGG | 0.611731 | 7.1:-7304768 | MS.gene09850:CDS |
| ATCAAGAGAGTCAAATTCAG+AGG | 0.614512 | 7.1:+7303924 | None:intergenic |
| ATGTAAAGAAAGGTAACAAG+GGG | 0.626414 | 7.1:-7304053 | MS.gene09850:CDS |
| TGATGAGCAGAATGCTCATG+AGG | 0.641178 | 7.1:+7304616 | None:intergenic |
| AAGGGGAATTCAACCCGAGG+GGG | 0.642568 | 7.1:-7304036 | MS.gene09850:CDS |
| GAAAGAATCAAAACCCAACA+TGG | 0.643797 | 7.1:-7304734 | MS.gene09850:CDS |
| CGTCGCATGAGGAATTGGAA+AGG | 0.653621 | 7.1:+7304690 | None:intergenic |
| ACAGTGTGCGACACTAGTGT+AGG | 0.663479 | 7.1:+7304126 | None:intergenic |
| TGTAGCCAAGATTCAAACTG+CGG | 0.664125 | 7.1:-7303898 | MS.gene09850:CDS |
| GTAGCCAAGGAAAACTCAAG+TGG | 0.667603 | 7.1:-7304769 | MS.gene09850:CDS |
| AACAAGGGGAATTCAACCCG+AGG | 0.692269 | 7.1:-7304039 | MS.gene09850:CDS |
| ACAAGGGGAATTCAACCCGA+GGG | 0.697575 | 7.1:-7304038 | MS.gene09850:CDS |
| AGCCAAGGAAAACTCAAGTG+GGG | 0.705918 | 7.1:-7304767 | MS.gene09850:CDS |
| CAAGGGGAATTCAACCCGAG+GGG | 0.745728 | 7.1:-7304037 | MS.gene09850:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGGCTAAATTAAAAAAAAAA+CGG | + | chr7.1:7304439-7304458 | None:intergenic | 15.0% |
| !! | AGTTTAACACTATATTGAAT+AGG | + | chr7.1:7304345-7304364 | None:intergenic | 20.0% |
| !! | ATTGAATAGGTAAATTCAAT+AGG | + | chr7.1:7304332-7304351 | None:intergenic | 20.0% |
| !! | GAAAAGAAAAATGTAAAGAA+AGG | - | chr7.1:7304675-7304694 | MS.gene09850:CDS | 20.0% |
| !! | GATGTAAAATACATAGAAAA+AGG | + | chr7.1:7304483-7304502 | None:intergenic | 20.0% |
| !! | TCAATATAGTGTTAAACTAA+TGG | - | chr7.1:7304347-7304366 | MS.gene09850:intron | 20.0% |
| ! | AAATGTAAAGAAAGGTAACA+AGG | - | chr7.1:7304683-7304702 | MS.gene09850:CDS | 25.0% |
| ! | AATGTAAAGAAAGGTAACAA+GGG | - | chr7.1:7304684-7304703 | MS.gene09850:CDS | 25.0% |
| ! | TTAATGATGCTTATGTGTAT+TGG | - | chr7.1:7304201-7304220 | MS.gene09850:CDS | 25.0% |
| ! | TTGTAACATGAAAAACTTAC+AGG | + | chr7.1:7304173-7304192 | None:intergenic | 25.0% |
| !! | AGTTTTTCATGTTACAAAGT+CGG | - | chr7.1:7304176-7304195 | MS.gene09850:CDS | 25.0% |
| !! | TTCTATGTATTTTACATCAC+AGG | - | chr7.1:7304485-7304504 | MS.gene09850:intron | 25.0% |
| !!! | AATTTCTGTTTTGAAAAAGC+TGG | - | chr7.1:7304245-7304264 | MS.gene09850:intron | 25.0% |
| ATGTAAAGAAAGGTAACAAG+GGG | - | chr7.1:7304685-7304704 | MS.gene09850:CDS | 30.0% | |
| CAACAAATCAATACCACATA+TGG | + | chr7.1:7304278-7304297 | None:intergenic | 30.0% | |
| TGTCCGAATAACAAAAAGAA+CGG | + | chr7.1:7304384-7304403 | None:intergenic | 30.0% | |
| ! | CTTTTCATTCTTCTTTTTCC+TGG | + | chr7.1:7304661-7304680 | None:intergenic | 30.0% |
| ! | TTCTTCTTTTTCCTGGATTT+CGG | + | chr7.1:7304654-7304673 | None:intergenic | 30.0% |
| !! | CATATGTGGTATTGATTTGT+TGG | - | chr7.1:7304276-7304295 | MS.gene09850:intron | 30.0% |
| !! | TTTTTCAAACACACCATGTT+GGG | + | chr7.1:7304020-7304039 | None:intergenic | 30.0% |
| !!! | ACACCGTTCTTTTTGTTATT+CGG | - | chr7.1:7304378-7304397 | MS.gene09850:intron | 30.0% |
| !!! | TTTTTTCAAACACACCATGT+TGG | + | chr7.1:7304021-7304040 | None:intergenic | 30.0% |
| AAAGGTAGCCGTGATAAAAA+CGG | - | chr7.1:7304534-7304553 | MS.gene09850:intron | 35.0% | |
| AGCTGTTGAAAAAAATGCAC+AGG | + | chr7.1:7304459-7304478 | None:intergenic | 35.0% | |
| ATAAAAACGGTGAACCACAT+AGG | - | chr7.1:7304547-7304566 | MS.gene09850:intron | 35.0% | |
| ATAGGAAATTGACGGTGAAA+TGG | - | chr7.1:7304565-7304584 | MS.gene09850:intron | 35.0% | |
| ATCAAGAGAGTCAAATTCAG+AGG | + | chr7.1:7304817-7304836 | None:intergenic | 35.0% | |
| CAATAGGAATGCTTAACAGA+TGG | + | chr7.1:7304316-7304335 | None:intergenic | 35.0% | |
| GAAAGAATCAAAACCCAACA+TGG | - | chr7.1:7304004-7304023 | MS.gene09850:CDS | 35.0% | |
| TAGGAAATTGACGGTGAAAT+GGG | - | chr7.1:7304566-7304585 | MS.gene09850:intron | 35.0% | |
| TGAACCACATAGGAAATTGA+CGG | - | chr7.1:7304557-7304576 | MS.gene09850:intron | 35.0% | |
| TGGGATTCTCAAGTTATTGA+AGG | - | chr7.1:7304789-7304808 | MS.gene09850:CDS | 35.0% | |
| TAGCCAAGGAAAACTCAAGT+GGG | - | chr7.1:7303970-7303989 | MS.gene09850:CDS | 40.0% | |
| TGTAGCCAAGATTCAAACTG+CGG | - | chr7.1:7304840-7304859 | MS.gene09850:CDS | 40.0% | |
| TTCACCGTCAATTTCCTATG+TGG | + | chr7.1:7304564-7304583 | None:intergenic | 40.0% | |
| TTTATCACGGCTACCTTTCA+AGG | + | chr7.1:7304532-7304551 | None:intergenic | 40.0% | |
| ! | AGAAGTGCATTACTCAGTTG+CGG | - | chr7.1:7304890-7304909 | MS.gene09850:CDS | 40.0% |
| ! | TTTTACATCACAGGCTCCTT+CGG | - | chr7.1:7304494-7304513 | MS.gene09850:intron | 40.0% |
| !! | GTGGTTCACCGTTTTTATCA+CGG | + | chr7.1:7304545-7304564 | None:intergenic | 40.0% |
| ACAAGAAACAGCCGAAATCC+AGG | - | chr7.1:7304640-7304659 | MS.gene09850:CDS | 45.0% | |
| AGCCAAGGAAAACTCAAGTG+GGG | - | chr7.1:7303971-7303990 | MS.gene09850:CDS | 45.0% | |
| AGCTGGCGTTATTCCATATG+TGG | - | chr7.1:7304262-7304281 | MS.gene09850:intron | 45.0% | |
| GAGCTCAAATCTGCCTTGAA+AGG | - | chr7.1:7304516-7304535 | MS.gene09850:intron | 45.0% | |
| GTAGCCAAGGAAAACTCAAG+TGG | - | chr7.1:7303969-7303988 | MS.gene09850:CDS | 45.0% | |
| TGATCTGTGCAGTAAGTCGT+GGG | - | chr7.1:7304770-7304789 | MS.gene09850:CDS | 45.0% | |
| TGATGAGCAGAATGCTCATG+AGG | + | chr7.1:7304125-7304144 | None:intergenic | 45.0% | |
| TGCGACACTAGTGTAGGTAT+AGG | + | chr7.1:7304609-7304628 | None:intergenic | 45.0% | |
| TGCTTCCGCAGTTTGAATCT+TGG | + | chr7.1:7304848-7304867 | None:intergenic | 45.0% | |
| ! | AACCCCACTTGAGTTTTCCT+TGG | + | chr7.1:7303976-7303995 | None:intergenic | 45.0% |
| AACAAGGGGAATTCAACCCG+AGG | - | chr7.1:7304699-7304718 | MS.gene09850:CDS | 50.0% | |
| AAGAAGCAGTCTCGCAACGT+TGG | - | chr7.1:7304738-7304757 | MS.gene09850:CDS | 50.0% | |
| AAGGCAGATTTGAGCTCCGA+AGG | + | chr7.1:7304513-7304532 | None:intergenic | 50.0% | |
| ACAAGGGGAATTCAACCCGA+GGG | - | chr7.1:7304700-7304719 | MS.gene09850:CDS | 50.0% | |
| ACAGTGTGCGACACTAGTGT+AGG | + | chr7.1:7304615-7304634 | None:intergenic | 50.0% | |
| CGTCGCATGAGGAATTGGAA+AGG | + | chr7.1:7304051-7304070 | None:intergenic | 50.0% | |
| CTGATCTGTGCAGTAAGTCG+TGG | - | chr7.1:7304769-7304788 | MS.gene09850:CDS | 50.0% | |
| TATCTTTGCCATTGCCCCCT+CGG | + | chr7.1:7304719-7304738 | None:intergenic | 50.0% | |
| TCCTCGTCAGAAGAGCTTGA+AGG | + | chr7.1:7304077-7304096 | None:intergenic | 50.0% | |
| TGCGATGTGCAATGTAGCCA+AGG | - | chr7.1:7303956-7303975 | MS.gene09850:CDS | 50.0% | |
| AAGCAGTCTCGCAACGTTGG+TGG | - | chr7.1:7304741-7304760 | MS.gene09850:CDS | 55.0% | |
| AAGGGGAATTCAACCCGAGG+GGG | - | chr7.1:7304702-7304721 | MS.gene09850:CDS | 55.0% | |
| AATTCAACCCGAGGGGGCAA+TGG | - | chr7.1:7304708-7304727 | MS.gene09850:CDS | 55.0% | |
| AGCAGTCTCGCAACGTTGGT+GGG | - | chr7.1:7304742-7304761 | MS.gene09850:CDS | 55.0% | |
| ATCTTTGCCATTGCCCCCTC+GGG | + | chr7.1:7304718-7304737 | None:intergenic | 55.0% | |
| CAAGGGGAATTCAACCCGAG+GGG | - | chr7.1:7304701-7304720 | MS.gene09850:CDS | 55.0% | |
| CTTGAAGGCAACGTCGCATG+AGG | + | chr7.1:7304062-7304081 | None:intergenic | 55.0% | |
| GCCTTCAAGCTCTTCTGACG+AGG | - | chr7.1:7304073-7304092 | MS.gene09850:CDS | 55.0% | |
| GGCAACGTCGCATGAGGAAT+TGG | + | chr7.1:7304056-7304075 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 7303837 | 7304923 | 7303837 | ID=MS.gene09850 |
| chr7.1 | mRNA | 7303837 | 7304923 | 7303837 | ID=MS.gene09850.t1;Parent=MS.gene09850 |
| chr7.1 | exon | 7304588 | 7304923 | 7304588 | ID=MS.gene09850.t1.exon1;Parent=MS.gene09850.t1 |
| chr7.1 | CDS | 7304588 | 7304923 | 7304588 | ID=cds.MS.gene09850.t1;Parent=MS.gene09850.t1 |
| chr7.1 | exon | 7303837 | 7304253 | 7303837 | ID=MS.gene09850.t1.exon2;Parent=MS.gene09850.t1 |
| chr7.1 | CDS | 7303837 | 7304253 | 7303837 | ID=cds.MS.gene09850.t1;Parent=MS.gene09850.t1 |
| Gene Sequence |
| Protein sequence |
