Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64517.t1 | XP_024625861.1 | 92.4 | 251 | 18 | 1 | 1 | 250 | 1 | 251 | 1.70E-101 | 379 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64517.t1 | G7L0L2 | 92.4 | 251 | 18 | 1 | 1 | 250 | 85 | 335 | 1.2e-101 | 379.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049580 | MS.gene64517 | 0.80769 | 4.47E-50 | -1.69E-46 |
| MS.gene049825 | MS.gene64517 | 0.840132 | 1.05E-57 | -1.69E-46 |
| MS.gene049873 | MS.gene64517 | 0.845099 | 5.02E-59 | -1.69E-46 |
| MS.gene050799 | MS.gene64517 | 0.804395 | 2.21E-49 | -1.69E-46 |
| MS.gene050944 | MS.gene64517 | 0.800966 | 1.12E-48 | -1.69E-46 |
| MS.gene051678 | MS.gene64517 | 0.820857 | 5.49E-53 | -1.69E-46 |
| MS.gene052208 | MS.gene64517 | 0.800201 | 1.61E-48 | -1.69E-46 |
| MS.gene052286 | MS.gene64517 | 0.804923 | 1.71E-49 | -1.69E-46 |
| MS.gene052358 | MS.gene64517 | -0.850904 | 1.26E-60 | -1.69E-46 |
| MS.gene052458 | MS.gene64517 | 0.814615 | 1.41E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene64517 | 0.815614 | 8.45E-52 | -1.69E-46 |
| MS.gene053006 | MS.gene64517 | 0.830841 | 2.34E-55 | -1.69E-46 |
| MS.gene053294 | MS.gene64517 | 0.84995 | 2.33E-60 | -1.69E-46 |
| MS.gene053401 | MS.gene64517 | 0.839673 | 1.38E-57 | -1.69E-46 |
| MS.gene053900 | MS.gene64517 | 0.807172 | 5.75E-50 | -1.69E-46 |
| MS.gene053995 | MS.gene64517 | 0.834246 | 3.35E-56 | -1.69E-46 |
| MS.gene055374 | MS.gene64517 | 0.861331 | 1.11E-63 | -1.69E-46 |
| MS.gene05548 | MS.gene64517 | 0.861437 | 1.03E-63 | -1.69E-46 |
| MS.gene055555 | MS.gene64517 | 0.835899 | 1.28E-56 | -1.69E-46 |
| MS.gene055808 | MS.gene64517 | 0.824766 | 6.76E-54 | -1.69E-46 |
| MS.gene055818 | MS.gene64517 | 0.808391 | 3.17E-50 | -1.69E-46 |
| MS.gene056091 | MS.gene64517 | 0.842958 | 1.88E-58 | -1.69E-46 |
| MS.gene056366 | MS.gene64517 | 0.802712 | 4.92E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene64517 | 0.820472 | 6.74E-53 | -1.69E-46 |
| MS.gene057079 | MS.gene64517 | 0.808544 | 2.94E-50 | -1.69E-46 |
| MS.gene057705 | MS.gene64517 | 0.845 | 5.33E-59 | -1.69E-46 |
| MS.gene05795 | MS.gene64517 | 0.831017 | 2.12E-55 | -1.69E-46 |
| MS.gene058037 | MS.gene64517 | 0.818061 | 2.39E-52 | -1.69E-46 |
| MS.gene058590 | MS.gene64517 | 0.81034 | 1.21E-50 | -1.69E-46 |
| MS.gene058635 | MS.gene64517 | 0.817355 | 3.44E-52 | -1.69E-46 |
| MS.gene05954 | MS.gene64517 | 0.837965 | 3.81E-57 | -1.69E-46 |
| MS.gene060572 | MS.gene64517 | 0.815783 | 7.75E-52 | -1.69E-46 |
| MS.gene060917 | MS.gene64517 | 0.825715 | 4.03E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene64517 | MS.gene068527 | PPI |
| MS.gene24068 | MS.gene64517 | PPI |
| MS.gene64517 | MS.gene058978 | PPI |
| MS.gene64517 | MS.gene018558 | PPI |
| MS.gene061900 | MS.gene64517 | PPI |
| MS.gene09850 | MS.gene64517 | PPI |
| MS.gene073852 | MS.gene64517 | PPI |
| MS.gene53776 | MS.gene64517 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64517.t1 | MTR_7g099950 | 92.430 | 251 | 18 | 1 | 1 | 250 | 85 | 335 | 9.03e-139 | 393 |
| MS.gene64517.t1 | MTR_1g068825 | 45.312 | 256 | 122 | 5 | 1 | 250 | 1 | 244 | 1.22e-47 | 158 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64517.t1 | AT2G37480 | 40.336 | 119 | 48 | 4 | 80 | 193 | 71 | 171 | 1.71e-13 | 67.4 |
| MS.gene64517.t1 | AT3G53670 | 36.800 | 125 | 71 | 3 | 68 | 192 | 61 | 177 | 1.96e-13 | 67.4 |
| MS.gene64517.t1 | AT3G53670 | 36.800 | 125 | 71 | 3 | 68 | 192 | 61 | 177 | 1.96e-13 | 67.4 |
| MS.gene64517.t1 | AT2G37480 | 40.336 | 119 | 49 | 3 | 80 | 193 | 71 | 172 | 4.01e-13 | 66.6 |
Find 38 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTCCGCAGTTTGAATCT+TGG | 0.342574 | 7.3:-12635922 | None:intergenic |
| GGCAACGTCGCATGAGGAAT+TGG | 0.368193 | 7.3:-12635130 | None:intergenic |
| ATAGGAAATTGACGGTGAAA+TGG | 0.412052 | 7.3:+12635642 | MS.gene64517:CDS |
| TTTATCACGGCTACCTTTCA+AGG | 0.425025 | 7.3:-12635606 | None:intergenic |
| ACAAGAAACAGCCGAAATCC+AGG | 0.447680 | 7.3:+12635717 | MS.gene64517:CDS |
| TAGGAAATTGACGGTGAAAT+GGG | 0.449608 | 7.3:+12635643 | MS.gene64517:CDS |
| TGGGATTCTCAAGTTATTGA+AGG | 0.461070 | 7.3:+12635866 | MS.gene64517:CDS |
| AAGCAGTCTCGCAACGTTGG+TGG | 0.501681 | 7.3:+12635818 | MS.gene64517:CDS |
| TCCTCGTCAGAAGAGCTTGA+AGG | 0.508539 | 7.3:-12635151 | None:intergenic |
| AAGGCAGATTTGAGCTCCGA+AGG | 0.526871 | 7.3:-12635587 | None:intergenic |
| AGCAGTCTCGCAACGTTGGT+GGG | 0.540201 | 7.3:+12635819 | MS.gene64517:CDS |
| GCCTTCAAGCTCTTCTGACG+AGG | 0.541579 | 7.3:+12635150 | MS.gene64517:CDS |
| TGCGACACTAGTGTAGGTAT+AGG | 0.544332 | 7.3:-12635683 | None:intergenic |
| TTCACCGTCAATTTCCTATG+TGG | 0.544822 | 7.3:-12635638 | None:intergenic |
| AAATGTAAAGAAAGGTAACA+AGG | 0.560400 | 7.3:+12635760 | MS.gene64517:CDS |
| AATGTAAAGAAAGGTAACAA+GGG | 0.568123 | 7.3:+12635761 | MS.gene64517:CDS |
| GAGCTCAAATCTGCCTTGAA+AGG | 0.568787 | 7.3:+12635593 | MS.gene64517:CDS |
| TGAACCACATAGGAAATTGA+CGG | 0.581672 | 7.3:+12635634 | MS.gene64517:CDS |
| CTGATCTGTGCAGTAAGTCG+TGG | 0.587620 | 7.3:+12635846 | MS.gene64517:CDS |
| TGCGATGTGCAATGTAGCCA+AGG | 0.589787 | 7.3:+12635033 | MS.gene64517:CDS |
| CTTGAAGGCAACGTCGCATG+AGG | 0.604027 | 7.3:-12635136 | None:intergenic |
| AGAAGTGCATTACTCAGTTG+CGG | 0.604827 | 7.3:+12635967 | MS.gene64517:CDS |
| TGATCTGTGCAGTAAGTCGT+GGG | 0.608135 | 7.3:+12635847 | MS.gene64517:CDS |
| AAGAAGCAGTCTCGCAACGT+TGG | 0.609694 | 7.3:+12635815 | MS.gene64517:CDS |
| TAGCCAAGGAAAACTCAAGT+GGG | 0.611731 | 7.3:+12635047 | MS.gene64517:CDS |
| ATCAAGAGAGTCAAATTCAG+AGG | 0.614512 | 7.3:-12635891 | None:intergenic |
| ATGTAAAGAAAGGTAACAAG+GGG | 0.626414 | 7.3:+12635762 | MS.gene64517:CDS |
| TGATGAGCAGAATGCTCATG+AGG | 0.641178 | 7.3:-12635199 | None:intergenic |
| AAGGGGAATTCAACCCGAGG+GGG | 0.642568 | 7.3:+12635779 | MS.gene64517:CDS |
| GAAAGAATCAAAACCCAACA+TGG | 0.643797 | 7.3:+12635081 | MS.gene64517:CDS |
| CGTCGCATGAGGAATTGGAA+AGG | 0.653621 | 7.3:-12635125 | None:intergenic |
| ACAGTGTGCGACACTAGTGT+AGG | 0.663479 | 7.3:-12635689 | None:intergenic |
| TGTAGCCAAGATTCAAACTG+CGG | 0.664125 | 7.3:+12635917 | MS.gene64517:CDS |
| GTAGCCAAGGAAAACTCAAG+TGG | 0.667603 | 7.3:+12635046 | MS.gene64517:CDS |
| AACAAGGGGAATTCAACCCG+AGG | 0.692269 | 7.3:+12635776 | MS.gene64517:CDS |
| ACAAGGGGAATTCAACCCGA+GGG | 0.697575 | 7.3:+12635777 | MS.gene64517:CDS |
| AGCCAAGGAAAACTCAAGTG+GGG | 0.705918 | 7.3:+12635048 | MS.gene64517:CDS |
| CAAGGGGAATTCAACCCGAG+GGG | 0.745728 | 7.3:+12635778 | MS.gene64517:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGGCTAAATTAAAAAAAAAA+CGG | - | chr7.3:12635516-12635535 | None:intergenic | 15.0% |
| !! | AGTTTAACACTATATTGAAT+AGG | - | chr7.3:12635422-12635441 | None:intergenic | 20.0% |
| !! | ATTGAATAGGTAAATTCAAT+AGG | - | chr7.3:12635409-12635428 | None:intergenic | 20.0% |
| !! | GAAAAGAAAAATGTAAAGAA+AGG | + | chr7.3:12635752-12635771 | MS.gene64517:CDS | 20.0% |
| !! | GATGTAAAATACATAGAAAA+AGG | - | chr7.3:12635560-12635579 | None:intergenic | 20.0% |
| !! | TCAATATAGTGTTAAACTAA+TGG | + | chr7.3:12635424-12635443 | MS.gene64517:intron | 20.0% |
| ! | AAATGTAAAGAAAGGTAACA+AGG | + | chr7.3:12635760-12635779 | MS.gene64517:CDS | 25.0% |
| ! | AATGTAAAGAAAGGTAACAA+GGG | + | chr7.3:12635761-12635780 | MS.gene64517:CDS | 25.0% |
| ! | TTAATGATGCTTATGTGTAT+TGG | + | chr7.3:12635278-12635297 | MS.gene64517:intron | 25.0% |
| ! | TTGTAACATGAAAAACTTAC+AGG | - | chr7.3:12635250-12635269 | None:intergenic | 25.0% |
| !! | AGTTTTTCATGTTACAAAGT+CGG | + | chr7.3:12635253-12635272 | MS.gene64517:intron | 25.0% |
| !! | TTCTATGTATTTTACATCAC+AGG | + | chr7.3:12635562-12635581 | MS.gene64517:intron | 25.0% |
| !!! | AATTTCTGTTTTGAAAAAGC+TGG | + | chr7.3:12635322-12635341 | MS.gene64517:intron | 25.0% |
| ATGTAAAGAAAGGTAACAAG+GGG | + | chr7.3:12635762-12635781 | MS.gene64517:CDS | 30.0% | |
| CAACAAATCAATACCACATA+TGG | - | chr7.3:12635355-12635374 | None:intergenic | 30.0% | |
| TGTCCGAATAACAAAAAGAA+CGG | - | chr7.3:12635461-12635480 | None:intergenic | 30.0% | |
| ! | CTTTTCATTCTTCTTTTTCC+TGG | - | chr7.3:12635738-12635757 | None:intergenic | 30.0% |
| ! | TTCTTCTTTTTCCTGGATTT+CGG | - | chr7.3:12635731-12635750 | None:intergenic | 30.0% |
| !! | CATATGTGGTATTGATTTGT+TGG | + | chr7.3:12635353-12635372 | MS.gene64517:intron | 30.0% |
| !! | TTTTTCAAACACACCATGTT+GGG | - | chr7.3:12635097-12635116 | None:intergenic | 30.0% |
| !!! | ACACCGTTCTTTTTGTTATT+CGG | + | chr7.3:12635455-12635474 | MS.gene64517:intron | 30.0% |
| !!! | TTTTTTCAAACACACCATGT+TGG | - | chr7.3:12635098-12635117 | None:intergenic | 30.0% |
| AAAGGTAGCCGTGATAAAAA+CGG | + | chr7.3:12635611-12635630 | MS.gene64517:CDS | 35.0% | |
| AGCTGTTGAAAAAAATGCAC+AGG | - | chr7.3:12635536-12635555 | None:intergenic | 35.0% | |
| ATAAAAACGGTGAACCACAT+AGG | + | chr7.3:12635624-12635643 | MS.gene64517:CDS | 35.0% | |
| ATAGGAAATTGACGGTGAAA+TGG | + | chr7.3:12635642-12635661 | MS.gene64517:CDS | 35.0% | |
| ATCAAGAGAGTCAAATTCAG+AGG | - | chr7.3:12635894-12635913 | None:intergenic | 35.0% | |
| CAATAGGAATGCTTAACAGA+TGG | - | chr7.3:12635393-12635412 | None:intergenic | 35.0% | |
| GAAAGAATCAAAACCCAACA+TGG | + | chr7.3:12635081-12635100 | MS.gene64517:CDS | 35.0% | |
| TAGGAAATTGACGGTGAAAT+GGG | + | chr7.3:12635643-12635662 | MS.gene64517:CDS | 35.0% | |
| TGAACCACATAGGAAATTGA+CGG | + | chr7.3:12635634-12635653 | MS.gene64517:CDS | 35.0% | |
| TGGGATTCTCAAGTTATTGA+AGG | + | chr7.3:12635866-12635885 | MS.gene64517:CDS | 35.0% | |
| TAGCCAAGGAAAACTCAAGT+GGG | + | chr7.3:12635047-12635066 | MS.gene64517:CDS | 40.0% | |
| TGTAGCCAAGATTCAAACTG+CGG | + | chr7.3:12635917-12635936 | MS.gene64517:CDS | 40.0% | |
| TTCACCGTCAATTTCCTATG+TGG | - | chr7.3:12635641-12635660 | None:intergenic | 40.0% | |
| TTTATCACGGCTACCTTTCA+AGG | - | chr7.3:12635609-12635628 | None:intergenic | 40.0% | |
| ! | AGAAGTGCATTACTCAGTTG+CGG | + | chr7.3:12635967-12635986 | MS.gene64517:CDS | 40.0% |
| ! | TTTTACATCACAGGCTCCTT+CGG | + | chr7.3:12635571-12635590 | MS.gene64517:intron | 40.0% |
| !! | GTGGTTCACCGTTTTTATCA+CGG | - | chr7.3:12635622-12635641 | None:intergenic | 40.0% |
| ACAAGAAACAGCCGAAATCC+AGG | + | chr7.3:12635717-12635736 | MS.gene64517:CDS | 45.0% | |
| AGCCAAGGAAAACTCAAGTG+GGG | + | chr7.3:12635048-12635067 | MS.gene64517:CDS | 45.0% | |
| AGCTGGCGTTATTCCATATG+TGG | + | chr7.3:12635339-12635358 | MS.gene64517:intron | 45.0% | |
| GAGCTCAAATCTGCCTTGAA+AGG | + | chr7.3:12635593-12635612 | MS.gene64517:CDS | 45.0% | |
| GTAGCCAAGGAAAACTCAAG+TGG | + | chr7.3:12635046-12635065 | MS.gene64517:CDS | 45.0% | |
| TGATCTGTGCAGTAAGTCGT+GGG | + | chr7.3:12635847-12635866 | MS.gene64517:CDS | 45.0% | |
| TGATGAGCAGAATGCTCATG+AGG | - | chr7.3:12635202-12635221 | None:intergenic | 45.0% | |
| TGCGACACTAGTGTAGGTAT+AGG | - | chr7.3:12635686-12635705 | None:intergenic | 45.0% | |
| TGCTTCCGCAGTTTGAATCT+TGG | - | chr7.3:12635925-12635944 | None:intergenic | 45.0% | |
| ! | AACCCCACTTGAGTTTTCCT+TGG | - | chr7.3:12635053-12635072 | None:intergenic | 45.0% |
| AACAAGGGGAATTCAACCCG+AGG | + | chr7.3:12635776-12635795 | MS.gene64517:CDS | 50.0% | |
| AAGAAGCAGTCTCGCAACGT+TGG | + | chr7.3:12635815-12635834 | MS.gene64517:CDS | 50.0% | |
| AAGGCAGATTTGAGCTCCGA+AGG | - | chr7.3:12635590-12635609 | None:intergenic | 50.0% | |
| ACAAGGGGAATTCAACCCGA+GGG | + | chr7.3:12635777-12635796 | MS.gene64517:CDS | 50.0% | |
| ACAGTGTGCGACACTAGTGT+AGG | - | chr7.3:12635692-12635711 | None:intergenic | 50.0% | |
| CGTCGCATGAGGAATTGGAA+AGG | - | chr7.3:12635128-12635147 | None:intergenic | 50.0% | |
| CTGATCTGTGCAGTAAGTCG+TGG | + | chr7.3:12635846-12635865 | MS.gene64517:CDS | 50.0% | |
| TATCTTTGCCATTGCCCCCT+CGG | - | chr7.3:12635796-12635815 | None:intergenic | 50.0% | |
| TCCTCGTCAGAAGAGCTTGA+AGG | - | chr7.3:12635154-12635173 | None:intergenic | 50.0% | |
| TGCGATGTGCAATGTAGCCA+AGG | + | chr7.3:12635033-12635052 | MS.gene64517:CDS | 50.0% | |
| AAGCAGTCTCGCAACGTTGG+TGG | + | chr7.3:12635818-12635837 | MS.gene64517:CDS | 55.0% | |
| AAGGGGAATTCAACCCGAGG+GGG | + | chr7.3:12635779-12635798 | MS.gene64517:CDS | 55.0% | |
| AATTCAACCCGAGGGGGCAA+TGG | + | chr7.3:12635785-12635804 | MS.gene64517:CDS | 55.0% | |
| AGCAGTCTCGCAACGTTGGT+GGG | + | chr7.3:12635819-12635838 | MS.gene64517:CDS | 55.0% | |
| ATCTTTGCCATTGCCCCCTC+GGG | - | chr7.3:12635795-12635814 | None:intergenic | 55.0% | |
| CAAGGGGAATTCAACCCGAG+GGG | + | chr7.3:12635778-12635797 | MS.gene64517:CDS | 55.0% | |
| CTTGAAGGCAACGTCGCATG+AGG | - | chr7.3:12635139-12635158 | None:intergenic | 55.0% | |
| GCCTTCAAGCTCTTCTGACG+AGG | + | chr7.3:12635150-12635169 | MS.gene64517:CDS | 55.0% | |
| GGCAACGTCGCATGAGGAAT+TGG | - | chr7.3:12635133-12635152 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 12634914 | 12636000 | 12634914 | ID=MS.gene64517 |
| chr7.3 | mRNA | 12634914 | 12636000 | 12634914 | ID=MS.gene64517.t1;Parent=MS.gene64517 |
| chr7.3 | exon | 12634914 | 12635249 | 12634914 | ID=MS.gene64517.t1.exon1;Parent=MS.gene64517.t1 |
| chr7.3 | CDS | 12634914 | 12635249 | 12634914 | ID=cds.MS.gene64517.t1;Parent=MS.gene64517.t1 |
| chr7.3 | exon | 12635584 | 12636000 | 12635584 | ID=MS.gene64517.t1.exon2;Parent=MS.gene64517.t1 |
| chr7.3 | CDS | 12635584 | 12636000 | 12635584 | ID=cds.MS.gene64517.t1;Parent=MS.gene64517.t1 |
| Gene Sequence |
| Protein sequence |
