AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051017

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051017.t1 MTR_8g023400 94.322 317 17 1 3 318 5 321 0.0 552
MS.gene051017.t1 MTR_7g117540 40.800 250 113 5 15 260 12 230 5.93e-45 155
MS.gene051017.t1 MTR_4g120270 42.135 178 76 7 19 178 13 181 1.47e-31 119
MS.gene051017.t1 MTR_2g436100 36.571 175 94 5 17 179 23 192 2.88e-30 116
MS.gene051017.t1 MTR_3g464780 38.150 173 88 4 27 180 26 198 5.56e-23 96.3
MS.gene051017.t1 MTR_5g064890 29.808 208 139 5 27 229 63 268 8.80e-23 97.4
MS.gene051017.t1 MTR_4g106680 35.385 195 92 8 27 201 48 228 4.67e-22 94.7
MS.gene051017.t1 MTR_5g034790 35.507 138 80 4 29 158 49 185 5.47e-22 95.5
MS.gene051017.t1 MTR_5g071930 39.860 143 68 3 27 163 7 137 8.93e-21 88.6
MS.gene051017.t1 MTR_8g063660 32.639 144 72 4 28 157 4 136 1.35e-20 90.9
MS.gene051017.t1 MTR_5g024730 31.285 179 97 4 28 202 33 189 1.63e-20 88.6
MS.gene051017.t1 MTR_4g129420 38.793 116 62 3 44 157 78 186 2.07e-20 92.0
MS.gene051017.t1 MTR_3g110190 52.778 72 33 1 90 160 66 137 8.03e-20 89.0
MS.gene051017.t1 MTR_6g087150 31.980 197 102 6 27 217 42 212 1.20e-19 89.0
MS.gene051017.t1 MTR_4g093830 32.584 178 107 4 30 194 58 235 1.48e-19 88.2
MS.gene051017.t1 MTR_1g100737 48.649 74 32 1 90 157 92 165 2.16e-19 84.7
MS.gene051017.t1 MTR_2g090950 36.306 157 85 3 12 160 19 168 4.72e-19 85.1
MS.gene051017.t1 MTR_7g113800 29.839 248 148 9 36 267 44 281 6.21e-19 86.7
MS.gene051017.t1 MTR_3g071300 31.210 157 89 5 17 168 17 159 8.35e-19 82.8
MS.gene051017.t1 MTR_6g007737 36.957 138 71 5 29 157 71 201 9.17e-19 86.3
MS.gene051017.t1 MTR_3g116000 44.737 76 42 0 90 165 79 154 1.01e-18 82.8
MS.gene051017.t1 MTR_8g092960 46.835 79 39 2 90 166 103 180 1.09e-18 83.6
MS.gene051017.t1 MTR_8g092840 46.835 79 39 2 90 166 113 190 1.11e-18 83.6
MS.gene051017.t1 MTR_5g066250 44.186 86 47 1 77 161 121 206 1.59e-18 85.9
MS.gene051017.t1 MTR_1g074190 33.824 136 75 4 36 164 53 180 1.68e-18 85.1
MS.gene051017.t1 MTR_8g089880 36.792 106 56 2 89 193 126 221 2.24e-18 84.0
MS.gene051017.t1 MTR_5g064990 35.971 139 67 5 26 157 62 185 3.21e-18 84.7
MS.gene051017.t1 MTR_7g073200 39.344 122 69 3 60 179 53 171 3.81e-18 80.9
MS.gene051017.t1 MTR_1g043410 35.461 141 79 5 30 162 34 170 4.36e-18 83.6
MS.gene051017.t1 MTR_3g094000 47.222 72 35 1 90 158 91 162 4.60e-18 80.9
MS.gene051017.t1 MTR_8g093010 41.935 93 50 3 76 166 94 184 5.66e-18 81.6
MS.gene051017.t1 MTR_7g076250 42.683 82 37 2 78 157 59 132 7.46e-18 80.5
MS.gene051017.t1 MTR_3g100400 48.000 75 38 1 90 163 130 204 1.20e-17 83.2
MS.gene051017.t1 MTR_2g090905 45.455 77 35 1 91 160 40 116 1.33e-17 78.2
MS.gene051017.t1 MTR_3g086730 32.000 175 83 5 27 186 1 154 1.62e-17 80.5
MS.gene051017.t1 MTR_2g096350 42.308 104 48 3 60 160 86 180 1.77e-17 80.1
MS.gene051017.t1 MTR_4g063070 35.252 139 69 6 33 157 247 378 1.78e-17 82.8
MS.gene051017.t1 MTR_8g092930 44.304 79 41 2 90 166 112 189 1.98e-17 80.1
MS.gene051017.t1 MTR_7g108855 37.857 140 67 5 68 203 47 170 2.27e-17 79.3
MS.gene051017.t1 MTR_8g092985 44.304 79 41 2 90 166 111 188 2.35e-17 79.7
MS.gene051017.t1 MTR_1g090480 32.593 135 80 3 29 158 17 145 2.51e-17 78.6
MS.gene051017.t1 MTR_8g073120 41.250 80 44 1 90 166 70 149 2.86e-17 79.3
MS.gene051017.t1 MTR_3g099650 35.915 142 68 7 26 160 57 182 3.87e-17 81.6
MS.gene051017.t1 MTR_3g086700 30.688 189 94 6 27 200 1 167 4.52e-17 78.2
MS.gene051017.t1 MTR_5g030010 31.387 137 89 3 28 160 58 193 4.79e-17 81.3
MS.gene051017.t1 MTR_3g095560 46.154 78 39 2 90 165 109 185 5.25e-17 79.3
MS.gene051017.t1 MTR_3g091620 40.230 87 46 2 79 164 95 176 5.99e-17 80.9
MS.gene051017.t1 MTR_6g055030 46.914 81 39 2 90 166 97 177 1.05e-16 79.7
MS.gene051017.t1 MTR_2g009310 43.038 79 44 1 80 157 125 203 1.33e-16 80.1
MS.gene051017.t1 MTR_1g082550 46.053 76 36 2 90 160 98 173 3.76e-16 78.6
MS.gene051017.t1 MTR_4g030070 46.479 71 34 3 89 157 84 152 5.53e-16 76.3
MS.gene051017.t1 MTR_5g082940 30.973 113 63 2 54 163 51 151 2.22e-15 73.2
MS.gene051017.t1 MTR_8g092990 52.830 53 24 1 114 166 140 191 3.03e-15 74.3
MS.gene051017.t1 MTR_8g092970 52.830 53 24 1 114 166 130 181 3.11e-15 73.9
MS.gene051017.t1 MTR_2g055790 47.059 68 34 1 90 157 77 142 3.39e-15 73.2
MS.gene051017.t1 MTR_3g114500 46.667 75 38 1 90 164 48 120 3.45e-15 71.6
MS.gene051017.t1 MTR_1g070140 41.892 74 41 1 90 161 103 176 4.21e-15 75.5
MS.gene051017.t1 MTR_1g026070 41.463 82 46 1 90 171 48 127 7.81e-15 70.5
MS.gene051017.t1 MTR_4g094725 42.105 76 41 1 90 162 78 153 1.01e-14 70.9
MS.gene051017.t1 MTR_1g492830 29.412 136 85 3 24 157 4 130 1.72e-14 72.0
MS.gene051017.t1 MTR_5g026730 59.574 47 19 0 111 157 90 136 2.42e-14 70.5
MS.gene051017.t1 MTR_6g007680 28.859 149 80 3 29 176 4 127 3.01e-14 69.7
MS.gene051017.t1 MTR_3g012550 38.961 77 44 1 85 158 75 151 4.06e-14 69.7
MS.gene051017.t1 MTR_8g066780 46.667 75 37 2 89 160 113 187 4.45e-14 72.4
MS.gene051017.t1 MTR_5g064980 43.056 72 37 1 90 157 108 179 4.89e-14 72.4
MS.gene051017.t1 MTR_7g116220 38.667 75 38 2 90 158 70 142 1.05e-13 68.9
MS.gene051017.t1 MTR_8g066740 38.710 93 45 3 90 175 76 163 1.49e-13 70.5
MS.gene051017.t1 MTR_5g026720 56.250 48 21 0 111 158 89 136 1.52e-13 67.8
MS.gene051017.t1 MTR_2g035800 42.466 73 39 2 90 160 76 147 1.75e-13 67.8
MS.gene051017.t1 MTR_4g056590 41.096 73 41 2 90 162 100 170 6.40e-13 67.0
MS.gene051017.t1 MTR_5g076990 29.221 154 87 6 27 175 27 163 2.02e-12 67.0
MS.gene051017.t1 MTR_7g096690 29.452 146 78 7 27 161 15 146 2.20e-12 67.0
MS.gene051017.t1 MTR_7g079280 35.616 73 44 1 89 158 79 151 2.38e-12 65.1
MS.gene051017.t1 MTR_1g090460 44.776 67 32 1 98 159 73 139 2.56e-12 64.3
MS.gene051017.t1 MTR_8g071815 46.875 64 30 1 115 174 90 153 2.70e-12 64.7
MS.gene051017.t1 MTR_8g072020 36.364 99 52 5 79 167 400 497 3.15e-12 67.4
MS.gene051017.t1 MTR_7g116180 38.356 73 41 2 90 158 67 139 3.25e-12 64.7
MS.gene051017.t1 MTR_8g072020 36.364 99 52 5 79 167 361 458 3.41e-12 67.4
MS.gene051017.t1 MTR_3g078450 36.000 75 45 1 87 158 73 147 4.07e-12 65.1
MS.gene051017.t1 MTR_8g028465 28.261 138 93 3 25 158 22 157 5.04e-12 64.3
MS.gene051017.t1 MTR_2g041200 61.364 44 17 0 114 157 108 151 5.10e-12 63.9
MS.gene051017.t1 MTR_1g070425 38.356 73 41 2 90 158 68 140 5.67e-12 63.9
MS.gene051017.t1 MTR_5g022920 57.778 45 19 0 113 157 187 231 5.81e-12 64.7
MS.gene051017.t1 MTR_7g116200 36.232 69 44 0 90 158 68 136 6.26e-12 63.5
MS.gene051017.t1 MTR_8g099495 51.111 45 22 0 115 159 347 391 6.57e-12 66.2
MS.gene051017.t1 MTR_7g059360 45.902 61 31 1 103 161 144 204 8.45e-12 64.3
MS.gene051017.t1 MTR_6g055030 56.000 50 22 0 117 166 97 146 8.80e-12 65.1
MS.gene051017.t1 MTR_7g079290 35.897 78 41 2 90 160 125 200 9.89e-12 63.9
MS.gene051017.t1 MTR_6g007670 30.769 104 65 1 96 199 14 110 1.03e-11 64.3
MS.gene051017.t1 MTR_8g032690 53.488 43 20 0 115 157 19 61 1.65e-11 60.8
MS.gene051017.t1 MTR_3g078470 36.000 75 45 1 87 158 73 147 2.37e-11 62.8
MS.gene051017.t1 MTR_4g022410 52.000 50 24 0 112 161 110 159 2.65e-11 62.0
MS.gene051017.t1 MTR_4g108830 42.857 70 38 2 90 158 121 189 2.68e-11 62.0
MS.gene051017.t1 MTR_4g108830 42.857 70 38 2 90 158 149 217 2.74e-11 62.8
MS.gene051017.t1 MTR_4g077570 49.020 51 22 2 110 157 6 55 3.75e-11 58.9
MS.gene051017.t1 MTR_5g009210 53.333 45 21 0 115 159 315 359 5.88e-11 63.2
MS.gene051017.t1 MTR_8g009190 39.130 69 38 1 93 157 79 147 5.95e-11 62.8
MS.gene051017.t1 MTR_7g116210 35.294 68 44 0 90 157 10 77 6.36e-11 59.3
MS.gene051017.t1 MTR_3g081030 44.444 72 36 3 88 157 92 161 6.42e-11 61.6
MS.gene051017.t1 MTR_1g096780 42.254 71 37 3 90 157 208 277 6.80e-11 63.2
MS.gene051017.t1 MTR_5g009200 53.333 45 21 0 115 159 350 394 7.46e-11 62.8
MS.gene051017.t1 MTR_3g087900 40.506 79 43 3 114 191 68 143 8.19e-11 60.1
MS.gene051017.t1 MTR_2g044570 53.191 47 22 0 114 160 82 128 8.46e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051017.t1 AT1G72310 40.064 312 144 6 14 302 11 302 1.55e-65 209
MS.gene051017.t1 AT1G53820 44.695 311 116 11 22 301 19 304 7.29e-65 207
MS.gene051017.t1 AT3G16720 36.824 296 140 12 18 308 21 274 2.86e-42 148
MS.gene051017.t1 AT3G62690 37.333 150 89 2 12 160 13 158 1.44e-26 105
MS.gene051017.t1 AT4G15975 43.103 116 54 3 45 160 18 121 5.93e-25 100
MS.gene051017.t1 AT3G14320 48.684 76 39 0 88 163 61 136 1.64e-23 96.3
MS.gene051017.t1 AT1G23980 36.296 135 81 3 28 157 52 186 5.57e-23 98.2
MS.gene051017.t1 AT5G17600 33.939 165 101 4 30 193 59 216 1.29e-22 97.1
MS.gene051017.t1 AT2G42360 36.111 144 77 2 19 160 23 153 2.94e-22 93.6
MS.gene051017.t1 AT5G05280 36.306 157 83 4 4 157 13 155 4.56e-22 91.7
MS.gene051017.t1 AT1G49220 47.170 106 45 4 68 166 84 185 5.13e-22 93.2
MS.gene051017.t1 AT2G47560 36.585 164 89 4 1 160 1 153 5.26e-22 92.8
MS.gene051017.t1 AT2G20030 39.437 142 72 4 23 163 44 172 4.82e-21 92.8
MS.gene051017.t1 AT1G04360 35.570 149 71 6 27 164 49 183 7.25e-21 92.4
MS.gene051017.t1 AT3G18773 41.584 101 56 2 90 188 103 202 3.15e-20 87.8
MS.gene051017.t1 AT3G48030 33.108 148 95 2 27 172 41 186 4.05e-20 88.6
MS.gene051017.t1 AT1G20823 33.766 154 85 5 17 165 20 161 5.42e-20 86.7
MS.gene051017.t1 AT4G40070 35.714 154 80 6 24 171 40 180 5.99e-20 89.0
MS.gene051017.t1 AT3G48030 33.108 148 95 2 27 172 119 264 9.13e-20 89.0
MS.gene051017.t1 AT1G49210 50.633 79 36 2 90 166 108 185 9.76e-20 86.7
MS.gene051017.t1 AT1G49200 48.810 84 40 2 85 166 104 186 1.16e-19 86.3
MS.gene051017.t1 AT5G05810 52.000 75 35 1 90 163 120 194 3.80e-19 87.8
MS.gene051017.t1 AT5G43420 31.841 201 108 7 16 197 31 221 3.83e-19 87.4
MS.gene051017.t1 AT1G76410 33.571 140 78 4 28 165 28 154 4.74e-19 83.6
MS.gene051017.t1 AT3G10910 44.565 92 48 2 90 179 88 178 5.36e-19 83.6
MS.gene051017.t1 AT2G35000 29.130 230 145 7 28 248 51 271 6.94e-19 86.7
MS.gene051017.t1 AT4G28890 44.792 96 50 2 90 184 97 190 7.24e-19 87.0
MS.gene051017.t1 AT3G19140 42.353 85 43 1 79 157 37 121 1.34e-18 81.3
MS.gene051017.t1 AT4G30400 42.157 102 52 3 61 157 77 176 1.68e-18 86.3
MS.gene051017.t1 AT5G40250 34.014 147 73 4 29 157 45 185 3.07e-18 84.7
MS.gene051017.t1 AT4G33565 37.736 106 65 1 80 184 181 286 6.93e-18 83.6
MS.gene051017.t1 AT1G72200 29.208 202 113 5 7 191 45 233 9.11e-18 83.6
MS.gene051017.t1 AT4G10150 30.769 130 88 1 28 157 26 153 1.03e-17 81.3
MS.gene051017.t1 AT3G03550 38.182 110 55 2 91 187 134 243 1.12e-17 82.8
MS.gene051017.t1 AT5G10380 52.857 70 32 1 89 157 108 177 1.13e-17 82.4
MS.gene051017.t1 AT5G01880 45.833 72 38 1 88 158 77 148 1.75e-17 78.6
MS.gene051017.t1 AT2G18650 45.977 87 44 3 78 161 87 173 2.53e-17 82.4
MS.gene051017.t1 AT4G17905 33.000 200 103 8 80 270 117 294 6.49e-17 80.1
MS.gene051017.t1 AT2G27940 31.343 134 89 1 28 158 50 183 7.86e-17 79.0
MS.gene051017.t1 AT4G17905 33.000 200 103 8 80 270 143 320 9.14e-17 80.1
MS.gene051017.t1 AT3G05200 37.500 104 56 3 90 184 99 202 9.61e-17 80.5
MS.gene051017.t1 AT1G33480 40.698 86 48 1 72 157 60 142 1.45e-16 78.6
MS.gene051017.t1 AT4G10160 42.857 70 40 0 88 157 70 139 1.46e-16 77.8
MS.gene051017.t1 AT4G09110 32.374 139 72 3 29 163 51 171 1.51e-16 79.0
MS.gene051017.t1 AT2G17450 38.938 113 56 3 71 175 55 162 1.68e-16 76.6
MS.gene051017.t1 AT1G72220 29.861 144 94 3 21 162 86 224 2.15e-16 79.7
MS.gene051017.t1 AT5G42200 31.544 149 80 4 15 157 14 146 2.31e-16 75.9
MS.gene051017.t1 AT4G09120 38.144 97 55 2 71 163 76 171 2.55e-16 79.0
MS.gene051017.t1 AT2G35420 32.812 128 76 4 33 157 25 145 2.70e-16 77.8
MS.gene051017.t1 AT1G49230 40.659 91 51 2 90 178 104 193 3.93e-16 76.6
MS.gene051017.t1 AT2G42350 30.496 141 82 1 17 157 18 142 7.30e-16 75.5
MS.gene051017.t1 AT5G57750 30.464 151 86 5 17 158 25 165 8.26e-16 75.5
MS.gene051017.t1 AT2G34990 43.056 72 37 1 90 157 89 160 1.05e-15 77.0
MS.gene051017.t1 AT2G34990 43.056 72 37 1 90 157 67 138 1.21e-15 76.6
MS.gene051017.t1 AT4G09130 37.363 91 53 2 78 164 79 169 2.37e-15 76.3
MS.gene051017.t1 AT1G32361 36.364 99 56 3 65 163 111 202 2.41e-15 75.9
MS.gene051017.t1 AT1G74410 42.500 80 44 2 83 160 142 221 3.64e-15 73.9
MS.gene051017.t1 AT5G27420 40.244 82 45 2 90 167 95 176 3.91e-15 75.9
MS.gene051017.t1 AT5G46650 35.211 142 71 7 28 157 25 157 5.08e-15 74.7
MS.gene051017.t1 AT5G07040 39.189 74 42 1 88 158 64 137 9.57e-15 71.2
MS.gene051017.t1 AT4G17920 39.394 99 55 2 90 184 81 178 1.49e-14 73.2
MS.gene051017.t1 AT3G18930 36.559 93 47 2 80 160 109 201 1.98e-14 73.9
MS.gene051017.t1 AT3G18930 36.559 93 47 2 80 160 109 201 1.98e-14 73.9
MS.gene051017.t1 AT3G18930 36.559 93 47 2 80 160 109 201 1.98e-14 73.9
MS.gene051017.t1 AT5G58580 39.286 112 60 3 90 193 111 222 6.20e-14 71.6
MS.gene051017.t1 AT1G22500 39.130 92 51 2 76 162 75 166 1.16e-13 71.2
MS.gene051017.t1 AT3G11110 30.667 150 87 3 17 164 22 156 1.67e-13 67.8
MS.gene051017.t1 AT1G35330 41.667 72 38 1 90 157 100 171 1.76e-13 70.5
MS.gene051017.t1 AT4G09100 42.667 75 39 1 90 160 56 130 2.29e-13 66.6
MS.gene051017.t1 AT3G61550 26.250 160 85 5 25 158 27 179 6.75e-13 67.4
MS.gene051017.t1 AT4G24015 44.737 76 39 2 102 175 91 165 1.15e-12 65.9
MS.gene051017.t1 AT5G66160 34.286 140 61 6 29 164 169 281 1.28e-12 67.8
MS.gene051017.t1 AT3G60220 38.372 86 46 2 80 158 75 160 1.57e-12 67.8
MS.gene051017.t1 AT2G46493 45.833 48 26 0 111 158 134 181 2.01e-12 65.5
MS.gene051017.t1 AT4G35480 38.889 72 44 0 108 179 106 177 2.20e-12 65.5
MS.gene051017.t1 AT5G66160 34.286 140 61 6 29 164 169 281 2.74e-12 66.6
MS.gene051017.t1 AT2G46160 30.612 98 57 2 72 158 84 181 2.79e-12 65.5
MS.gene051017.t1 AT2G46160 30.612 98 57 2 72 158 84 181 2.79e-12 65.5
MS.gene051017.t1 AT1G71980 36.782 87 51 3 90 173 205 290 4.81e-12 66.6
MS.gene051017.t1 AT5G06490 34.615 78 43 1 89 158 95 172 5.71e-12 64.3
MS.gene051017.t1 AT2G44578 58.140 43 18 0 115 157 70 112 6.37e-12 62.8
MS.gene051017.t1 AT2G46494 47.917 48 25 0 111 158 312 359 8.54e-12 65.9
MS.gene051017.t1 AT5G66070 54.348 46 21 0 113 158 174 219 8.61e-12 64.3
MS.gene051017.t1 AT5G66070 54.348 46 21 0 113 158 174 219 8.61e-12 64.3
MS.gene051017.t1 AT5G66070 55.556 45 20 0 113 157 198 242 1.41e-11 63.9
MS.gene051017.t1 AT2G17730 40.278 72 39 1 90 157 167 238 2.22e-11 63.2
MS.gene051017.t1 AT5G47610 41.270 63 35 1 100 160 90 152 2.22e-11 62.0
MS.gene051017.t1 AT5G53110 36.735 98 54 3 69 158 276 373 2.39e-11 64.3
MS.gene051017.t1 AT1G28040 45.833 48 26 0 111 158 249 296 2.48e-11 63.9
MS.gene051017.t1 AT4G30370 38.889 72 43 1 90 160 89 160 5.12e-11 61.2
MS.gene051017.t1 AT3G60966 60.465 43 17 0 115 157 62 104 6.97e-11 59.7
MS.gene051017.t1 AT2G44581 53.488 43 20 0 115 157 70 112 7.75e-11 59.7
MS.gene051017.t1 AT3G20395 36.111 72 45 1 90 161 147 217 7.97e-11 61.6
MS.gene051017.t1 AT2G46495 45.833 48 26 0 111 158 316 363 9.67e-11 62.8
MS.gene051017.t1 AT2G46495 45.833 48 26 0 111 158 288 335 9.90e-11 62.4

Find 52 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCAAGATCCTGTTGTTTA+TGG 0.168039 8.3:+68784705 MS.gene051017:CDS
CCATTTGAATCAGTACTATT+TGG 0.192512 8.3:-68784972 None:intergenic
TCTTAAGTCTTTGCCTGTTT+TGG 0.218884 8.3:+68784758 MS.gene051017:CDS
TGAAACCCATGATTGCATTT+TGG 0.226368 8.3:-68784867 None:intergenic
CGTCGACGACGGCGGTTTCT+TGG 0.237232 8.3:-68784663 None:intergenic
ATTCTACTTGTCCTCTTTGT+AGG 0.257676 8.3:+68784922 MS.gene051017:CDS
ATTTATATGCAAAGTGGTTT+TGG 0.278266 8.3:+68784610 MS.gene051017:CDS
TCCTTCAACTCCAAGTTCTA+TGG 0.291895 8.3:+68785385 MS.gene051017:CDS
AAATTTCCCTACAAATGTTT+TGG 0.305638 8.3:+68785064 MS.gene051017:CDS
GTTTATGGAAGTCACCAAAT+TGG 0.322819 8.3:+68784720 MS.gene051017:CDS
ACATCATCATCATCTACTTT+AGG 0.335783 8.3:+68785170 MS.gene051017:CDS
TGAGGATTTCAAAGATGGTT+TGG 0.341504 8.3:+68784791 MS.gene051017:CDS
ACACAAGTTAGTTCTTCTTT+TGG 0.347982 8.3:+68785104 MS.gene051017:CDS
AAAGGGTCAGGTGATGTATT+AGG 0.362982 8.3:+68784498 MS.gene051017:CDS
CAAGATTGAGATCATTTAAG+AGG 0.369842 8.3:+68785336 MS.gene051017:CDS
AGTAATAGATGCAATGGAAA+TGG 0.383393 8.3:+68785209 MS.gene051017:CDS
CCCCAAACCAAAACATTTGT+AGG 0.395435 8.3:-68785071 None:intergenic
TCTAATACAGTTGAAATAAC+AGG 0.400393 8.3:+68784525 MS.gene051017:CDS
GCAACTGCAACTGCAACAAA+AGG 0.404413 8.3:+68784480 MS.gene051017:CDS
TCTACTTTAGGTAGTGATAA+TGG 0.408867 8.3:+68785182 MS.gene051017:CDS
TGTCTTTGTGATGTTGTTGA+AGG 0.438454 8.3:+68784825 MS.gene051017:CDS
TCCATAGAACTTGGAGTTGA+AGG 0.444903 8.3:-68785386 None:intergenic
ATTTAGATTGTATTGATATG+TGG 0.449698 8.3:+68784889 MS.gene051017:CDS
TGGTGACTTCCATAAACAAC+AGG 0.454316 8.3:-68784714 None:intergenic
CGACGGCGGTTTCTTGGTTG+AGG 0.455007 8.3:-68784657 None:intergenic
TTTGGTGTTTCTTTGGAAGA+AGG 0.471878 8.3:+68785122 MS.gene051017:CDS
TGTTCCACATCCATAGAACT+TGG 0.475183 8.3:-68785395 None:intergenic
AACTCCAAGTTCTATGGATG+TGG 0.479911 8.3:+68785391 MS.gene051017:CDS
GAGGAAGAACTAAACTCTCT+TGG 0.492277 8.3:-68785257 None:intergenic
GGTAATAGTAATAGATGCAA+TGG 0.506680 8.3:+68785203 MS.gene051017:CDS
CCAAATAGTACTGATTCAAA+TGG 0.506743 8.3:+68784972 MS.gene051017:CDS
GTCACTAGCACTATTAGCTC+TGG 0.507539 8.3:-68785421 None:intergenic
AAACAAACCGCCGTCGACGA+CGG 0.534947 8.3:-68784674 None:intergenic
CCCAAACCAAAACATTTGTA+GGG 0.536138 8.3:-68785070 None:intergenic
TGCTTCATTTATATGCAAAG+TGG 0.539115 8.3:+68784604 MS.gene051017:CDS
GCAACTGCAACAAAAGGGTC+AGG 0.546145 8.3:+68784486 MS.gene051017:CDS
CAACTGCAACTGCAACAAAA+GGG 0.562538 8.3:+68784481 MS.gene051017:CDS
GATCTCAATCTTGTTGCCAT+TGG 0.564352 8.3:-68785326 None:intergenic
GAAACTAAATTCCTACAAAG+AGG 0.564972 8.3:-68784933 None:intergenic
ATGCAATGGAAATGGAATGT+TGG 0.570708 8.3:+68785217 MS.gene051017:CDS
CAAGAAACCGCCGTCGTCGA+CGG 0.576823 8.3:+68784664 MS.gene051017:CDS
AGTTCTATGGATGTGGAACA+AGG 0.581073 8.3:+68785398 MS.gene051017:CDS
GAACTAAACTCTCTTGGCAA+TGG 0.586066 8.3:-68785251 None:intergenic
GAAAATTTGAGTCATGTGAA+TGG 0.596814 8.3:+68785029 MS.gene051017:CDS
AGATGAAATGAAATCACCAA+TGG 0.606642 8.3:+68785310 MS.gene051017:CDS
TCAACACAGCTATTATCAGA+AGG 0.635451 8.3:-68785287 None:intergenic
CAAACCGCCGTCGACGACGG+CGG 0.650391 8.3:-68784671 None:intergenic
GAAACCGCCGTCGTCGACGG+CGG 0.652134 8.3:+68784667 MS.gene051017:CDS
ATTATCAGAAGGTGACAATG+AGG 0.653561 8.3:-68785276 None:intergenic
TAAACAACAGGATCTTGACC+AGG 0.667684 8.3:-68784702 None:intergenic
AATGTGTTAGTGTCATCAGA+AGG 0.695473 8.3:+68785005 MS.gene051017:CDS
GATGATGATGATGTTGAACA+AGG 0.699539 8.3:-68785161 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGCAATAATTTTTCTATTCA+TGG + chr8.3:68784563-68784582 MS.gene051017:CDS 20.0%
!!! ATTTAGATTGTATTGATATG+TGG + chr8.3:68784889-68784908 MS.gene051017:CDS 20.0%
! AAAAATGAGGATTTCAAAGA+TGG + chr8.3:68784786-68784805 MS.gene051017:CDS 25.0%
! TCTAATACAGTTGAAATAAC+AGG + chr8.3:68784525-68784544 MS.gene051017:CDS 25.0%
! TGAAATAACAGGAAAAATCA+TGG + chr8.3:68784536-68784555 MS.gene051017:CDS 25.0%
!! AAATTTCCCTACAAATGTTT+TGG + chr8.3:68785064-68785083 MS.gene051017:CDS 25.0%
!! ATTTATATGCAAAGTGGTTT+TGG + chr8.3:68784610-68784629 MS.gene051017:CDS 25.0%
!! TTTTACCAAAATGCAATCAT+GGG + chr8.3:68784862-68784881 MS.gene051017:CDS 25.0%
!!! TCATTTTTGAAAACCAAAAC+AGG - chr8.3:68784774-68784793 None:intergenic 25.0%
!!! TGTTTTGGTTTTCAAAAATG+AGG + chr8.3:68784773-68784792 MS.gene051017:CDS 25.0%
ACATCATCATCATCTACTTT+AGG + chr8.3:68785170-68785189 MS.gene051017:CDS 30.0%
AGATGAAATGAAATCACCAA+TGG + chr8.3:68785310-68785329 MS.gene051017:CDS 30.0%
AGTAATAGATGCAATGGAAA+TGG + chr8.3:68785209-68785228 MS.gene051017:CDS 30.0%
CAAGATTGAGATCATTTAAG+AGG + chr8.3:68785336-68785355 MS.gene051017:CDS 30.0%
CCATTTGAATCAGTACTATT+TGG - chr8.3:68784975-68784994 None:intergenic 30.0%
GAAAATTTGAGTCATGTGAA+TGG + chr8.3:68785029-68785048 MS.gene051017:CDS 30.0%
GAAACTAAATTCCTACAAAG+AGG - chr8.3:68784936-68784955 None:intergenic 30.0%
GGTAATAGTAATAGATGCAA+TGG + chr8.3:68785203-68785222 MS.gene051017:CDS 30.0%
TCTACTTTAGGTAGTGATAA+TGG + chr8.3:68785182-68785201 MS.gene051017:CDS 30.0%
TGCTTCATTTATATGCAAAG+TGG + chr8.3:68784604-68784623 MS.gene051017:CDS 30.0%
! CTTTTACCAAAATGCAATCA+TGG + chr8.3:68784861-68784880 MS.gene051017:CDS 30.0%
! GGCAAAGACTTAAGAATTTT+AGG - chr8.3:68784753-68784772 None:intergenic 30.0%
!! ACACAAGTTAGTTCTTCTTT+TGG + chr8.3:68785104-68785123 MS.gene051017:CDS 30.0%
!! CCAAATAGTACTGATTCAAA+TGG + chr8.3:68784972-68784991 MS.gene051017:CDS 30.0%
!!! TTCTTCTTTTGGTGTTTCTT+TGG + chr8.3:68785115-68785134 MS.gene051017:CDS 30.0%
!!! TTTTAGGTTCAAGTCCAATT+TGG - chr8.3:68784737-68784756 None:intergenic 30.0%
AATGTGTTAGTGTCATCAGA+AGG + chr8.3:68785005-68785024 MS.gene051017:CDS 35.0%
ATGCAATGGAAATGGAATGT+TGG + chr8.3:68785217-68785236 MS.gene051017:CDS 35.0%
ATTATCAGAAGGTGACAATG+AGG - chr8.3:68785279-68785298 None:intergenic 35.0%
ATTCTACTTGTCCTCTTTGT+AGG + chr8.3:68784922-68784941 MS.gene051017:CDS 35.0%
CCCAAACCAAAACATTTGTA+GGG - chr8.3:68785073-68785092 None:intergenic 35.0%
GTTTATGGAAGTCACCAAAT+TGG + chr8.3:68784720-68784739 MS.gene051017:CDS 35.0%
TCAACACAGCTATTATCAGA+AGG - chr8.3:68785290-68785309 None:intergenic 35.0%
TGAGGATTTCAAAGATGGTT+TGG + chr8.3:68784791-68784810 MS.gene051017:CDS 35.0%
TGTCTTTGTGATGTTGTTGA+AGG + chr8.3:68784825-68784844 MS.gene051017:CDS 35.0%
! GATGATGATGATGTTGAACA+AGG - chr8.3:68785164-68785183 None:intergenic 35.0%
! TCTTAAGTCTTTGCCTGTTT+TGG + chr8.3:68784758-68784777 MS.gene051017:CDS 35.0%
! TGAAACCCATGATTGCATTT+TGG - chr8.3:68784870-68784889 None:intergenic 35.0%
!! TTTGGTGTTTCTTTGGAAGA+AGG + chr8.3:68785122-68785141 MS.gene051017:CDS 35.0%
!!! CCCTACAAATGTTTTGGTTT+GGG + chr8.3:68785070-68785089 MS.gene051017:CDS 35.0%
!!! CCTACAAATGTTTTGGTTTG+GGG + chr8.3:68785071-68785090 MS.gene051017:CDS 35.0%
!!! CTACAAATGTTTTGGTTTGG+GGG + chr8.3:68785072-68785091 MS.gene051017:CDS 35.0%
!!! TATATGCAAAGTGGTTTTGG+TGG + chr8.3:68784613-68784632 MS.gene051017:CDS 35.0%
!!! TCCCTACAAATGTTTTGGTT+TGG + chr8.3:68785069-68785088 MS.gene051017:CDS 35.0%
AAAGGGTCAGGTGATGTATT+AGG + chr8.3:68784498-68784517 MS.gene051017:CDS 40.0%
AACTCCAAGTTCTATGGATG+TGG + chr8.3:68785391-68785410 MS.gene051017:CDS 40.0%
AGTTCTATGGATGTGGAACA+AGG + chr8.3:68785398-68785417 MS.gene051017:CDS 40.0%
CAACTGCAACTGCAACAAAA+GGG + chr8.3:68784481-68784500 MS.gene051017:CDS 40.0%
CCCCAAACCAAAACATTTGT+AGG - chr8.3:68785074-68785093 None:intergenic 40.0%
GAACTAAACTCTCTTGGCAA+TGG - chr8.3:68785254-68785273 None:intergenic 40.0%
GAGGAAGAACTAAACTCTCT+TGG - chr8.3:68785260-68785279 None:intergenic 40.0%
GATCTCAATCTTGTTGCCAT+TGG - chr8.3:68785329-68785348 None:intergenic 40.0%
TAAACAACAGGATCTTGACC+AGG - chr8.3:68784705-68784724 None:intergenic 40.0%
TCCATAGAACTTGGAGTTGA+AGG - chr8.3:68785389-68785408 None:intergenic 40.0%
TCCTTCAACTCCAAGTTCTA+TGG + chr8.3:68785385-68785404 MS.gene051017:CDS 40.0%
TGGTGACTTCCATAAACAAC+AGG - chr8.3:68784717-68784736 None:intergenic 40.0%
TGTTCCACATCCATAGAACT+TGG - chr8.3:68785398-68785417 None:intergenic 40.0%
! GGTCAAGATCCTGTTGTTTA+TGG + chr8.3:68784705-68784724 MS.gene051017:CDS 40.0%
GCAACTGCAACTGCAACAAA+AGG + chr8.3:68784480-68784499 MS.gene051017:CDS 45.0%
GCAACTGCAACAAAAGGGTC+AGG + chr8.3:68784486-68784505 MS.gene051017:CDS 50.0%
!!! GTGGTTTTGGTGGCGTTTTG+AGG + chr8.3:68784623-68784642 MS.gene051017:CDS 50.0%
AAACAAACCGCCGTCGACGA+CGG - chr8.3:68784677-68784696 None:intergenic 55.0%
!!! CGGCGGTTTGTTTTTGCTCC+TGG + chr8.3:68784684-68784703 MS.gene051017:CDS 55.0%
CAAGAAACCGCCGTCGTCGA+CGG + chr8.3:68784664-68784683 MS.gene051017:CDS 60.0%
CGACGGCGGTTTCTTGGTTG+AGG - chr8.3:68784660-68784679 None:intergenic 60.0%
CGTCGACGACGGCGGTTTCT+TGG - chr8.3:68784666-68784685 None:intergenic 65.0%
CAAACCGCCGTCGACGACGG+CGG - chr8.3:68784674-68784693 None:intergenic 70.0%
GAAACCGCCGTCGTCGACGG+CGG + chr8.3:68784667-68784686 MS.gene051017:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 68784474 68785430 68784474 ID=MS.gene051017
chr8.3 mRNA 68784474 68785430 68784474 ID=MS.gene051017.t1;Parent=MS.gene051017
chr8.3 exon 68784474 68785430 68784474 ID=MS.gene051017.t1.exon1;Parent=MS.gene051017.t1
chr8.3 CDS 68784474 68785430 68784474 ID=cds.MS.gene051017.t1;Parent=MS.gene051017.t1
Gene Sequence

>MS.gene051017

ATGGGTGCAACTGCAACTGCAACAAAAGGGTCAGGTGATGTATTAGGTGATTCTAATACAGTTGAAATAACAGGAAAAATCATGGTTTTAGCAATAATTTTTCTATTCATGGTTGTTATGTTTGTTCTAATGCTTCATTTATATGCAAAGTGGTTTTGGTGGCGTTTTGAGGAAAGAACTGTTCCTCAACCAAGAAACCGCCGTCGTCGACGGCGGTTTGTTTTTGCTCCTGGTCAAGATCCTGTTGTTTATGGAAGTCACCAAATTGGACTTGAACCTAAAATTCTTAAGTCTTTGCCTGTTTTGGTTTTCAAAAATGAGGATTTCAAAGATGGTTTGGAATGTGCTGTTTGTCTTTGTGATGTTGTTGAAGGAGAAAAAACTAGACTTTTACCAAAATGCAATCATGGGTTTCATTTAGATTGTATTGATATGTGGTTTCAATCTCATTCTACTTGTCCTCTTTGTAGGAATTTAGTTTCTGTTGAATCATGTAAACCAAATAGTACTGATTCAAATGGTGAAGAGATGAATGTGTTAGTGTCATCAGAAGGTGAAAATTTGAGTCATGTGAATGGTCTAGAGTCTTCAAATTTCCCTACAAATGTTTTGGTTTGGGGGAATTCTCAAACACAAGTTAGTTCTTCTTTTGGTGTTTCTTTGGAAGAAGGAAGTTCTCATCAACAACCTTGTTCAACATCATCATCATCTACTTTAGGTAGTGATAATGGTAATAGTAATAGATGCAATGGAAATGGAATGTTGGTGATTGATATACCATTGCCAAGAGAGTTTAGTTCTTCCTCATTGTCACCTTCTGATAATAGCTGTGTTGAAGATGAAATGAAATCACCAATGGCAACAAGATTGAGATCATTTAAGAGGCTTTTAAGTAGAGACAAAAAGTTTAGTCCTTCAACTCCAAGTTCTATGGATGTGGAACAAGGCCAGAGCTAA
Protein sequence

>MS.gene051017.t1

MGATATATKGSGDVLGDSNTVEITGKIMVLAIIFLFMVVMFVLMLHLYAKWFWWRFEERTVPQPRNRRRRRRFVFAPGQDPVVYGSHQIGLEPKILKSLPVLVFKNEDFKDGLECAVCLCDVVEGEKTRLLPKCNHGFHLDCIDMWFQSHSTCPLCRNLVSVESCKPNSTDSNGEEMNVLVSSEGENLSHVNGLESSNFPTNVLVWGNSQTQVSSSFGVSLEEGSSHQQPCSTSSSSTLGSDNGNSNRCNGNGMLVIDIPLPREFSSSSLSPSDNSCVEDEMKSPMATRLRSFKRLLSRDKKFSPSTPSSMDVEQGQS