Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051118.t1 | XP_003608925.1 | 79 | 248 | 7 | 1 | 1 | 203 | 1 | 248 | 1.80E-101 | 378.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051118.t1 | O48716 | 32.8 | 186 | 109 | 4 | 18 | 202 | 102 | 272 | 7.7e-21 | 102.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051118.t1 | G7JNR9 | 79.0 | 248 | 7 | 1 | 1 | 203 | 1 | 248 | 1.3e-101 | 378.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051118 | MS.gene30401 | 0.908853 | 1.08E-81 | -1.69E-46 |
| MS.gene051118 | MS.gene60331 | 0.806521 | 7.90E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051118.t1 | MTR_4g106480 | 78.601 | 243 | 7 | 1 | 1 | 198 | 1 | 243 | 1.09e-130 | 373 |
| MS.gene051118.t1 | MTR_3g078380 | 61.929 | 197 | 65 | 3 | 9 | 203 | 62 | 250 | 1.42e-80 | 246 |
| MS.gene051118.t1 | MTR_5g035980 | 54.255 | 188 | 78 | 4 | 23 | 203 | 82 | 268 | 7.19e-62 | 199 |
| MS.gene051118.t1 | MTR_5g018520 | 39.785 | 186 | 98 | 3 | 18 | 203 | 103 | 274 | 1.94e-43 | 151 |
| MS.gene051118.t1 | MTR_8g089650 | 49.677 | 155 | 70 | 4 | 49 | 203 | 131 | 277 | 3.96e-43 | 150 |
| MS.gene051118.t1 | MTR_1g021590 | 41.711 | 187 | 93 | 5 | 18 | 203 | 115 | 286 | 3.45e-42 | 148 |
| MS.gene051118.t1 | MTR_4g104410 | 45.213 | 188 | 89 | 6 | 18 | 203 | 43 | 218 | 7.46e-41 | 142 |
| MS.gene051118.t1 | MTR_4g068457 | 39.785 | 186 | 98 | 3 | 18 | 203 | 134 | 305 | 4.81e-39 | 140 |
| MS.gene051118.t1 | MTR_2g060070 | 37.097 | 186 | 102 | 5 | 18 | 203 | 70 | 240 | 8.96e-33 | 122 |
| MS.gene051118.t1 | MTR_2g101410 | 30.811 | 185 | 107 | 5 | 19 | 203 | 92 | 255 | 2.32e-19 | 85.5 |
| MS.gene051118.t1 | MTR_3g110185 | 31.250 | 192 | 103 | 10 | 18 | 203 | 94 | 262 | 2.29e-18 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051118.t1 | AT5G50120 | 57.436 | 195 | 79 | 2 | 13 | 203 | 39 | 233 | 2.03e-75 | 232 |
| MS.gene051118.t1 | AT1G24130 | 56.633 | 196 | 81 | 3 | 10 | 203 | 67 | 260 | 3.25e-73 | 227 |
| MS.gene051118.t1 | AT1G24530 | 49.655 | 145 | 64 | 4 | 59 | 203 | 125 | 260 | 4.51e-43 | 149 |
| MS.gene051118.t1 | AT2G26490 | 40.107 | 187 | 96 | 4 | 18 | 203 | 102 | 273 | 7.88e-41 | 144 |
| MS.gene051118.t1 | AT1G47610 | 40.426 | 188 | 94 | 6 | 18 | 203 | 30 | 201 | 3.51e-38 | 135 |
| MS.gene051118.t1 | AT3G50390 | 39.153 | 189 | 98 | 5 | 18 | 203 | 104 | 278 | 2.90e-37 | 135 |
| MS.gene051118.t1 | AT3G51930 | 39.247 | 186 | 99 | 6 | 18 | 203 | 67 | 238 | 2.67e-36 | 131 |
| MS.gene051118.t1 | AT1G49450 | 39.572 | 187 | 98 | 4 | 18 | 203 | 140 | 312 | 5.91e-36 | 131 |
| MS.gene051118.t1 | AT3G18950 | 38.503 | 187 | 100 | 4 | 18 | 203 | 144 | 316 | 1.80e-35 | 130 |
| MS.gene051118.t1 | AT4G34380 | 38.710 | 186 | 100 | 3 | 18 | 203 | 131 | 302 | 5.42e-34 | 126 |
Find 35 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGAGGGTGAGAATGAATA+TGG | 0.282172 | 8.3:+70268498 | None:intergenic |
| ATGTTGGGAGTGTGGCTAAA+TGG | 0.285118 | 8.3:+70268321 | None:intergenic |
| CCTTGTTATATTCTGTTTCA+TGG | 0.304199 | 8.3:-70268175 | MS.gene051118:CDS |
| AACCAAAGACTTCACGTGTT+TGG | 0.328930 | 8.3:-70268129 | MS.gene051118:CDS |
| TTCCAAACACGTGAAGTCTT+TGG | 0.336054 | 8.3:+70268127 | None:intergenic |
| AACACTAACACAATAGTATC+TGG | 0.378047 | 8.3:-70268467 | MS.gene051118:CDS |
| AAAACCATGTTAATATTAGA+AGG | 0.405172 | 8.3:-70268265 | MS.gene051118:CDS |
| TGTTGGGAGTGTGGCTAAAT+GGG | 0.428968 | 8.3:+70268322 | None:intergenic |
| AAGATAACAAAATCCGCGTT+TGG | 0.438563 | 8.3:-70268388 | MS.gene051118:CDS |
| CAAGGTTCCATCTTTCGATA+AGG | 0.440768 | 8.3:+70268193 | None:intergenic |
| GTGAAACGATCTATGAATGT+TGG | 0.451524 | 8.3:+70268305 | None:intergenic |
| AGACTTCACGTGTTTGGAAT+CGG | 0.453279 | 8.3:-70268123 | MS.gene051118:CDS |
| AACACAATAGTATCTGGAAA+TGG | 0.466792 | 8.3:-70268461 | MS.gene051118:CDS |
| GCAATCACAGTTTCTAATGA+TGG | 0.473911 | 8.3:-70268071 | MS.gene051118:CDS |
| TGAAACGATCTATGAATGTT+GGG | 0.478701 | 8.3:+70268306 | None:intergenic |
| CTTGTTATATTCTGTTTCAT+GGG | 0.480779 | 8.3:-70268174 | MS.gene051118:CDS |
| CCATGATGTGACTTCTGTGT+TGG | 0.496060 | 8.3:+70268529 | None:intergenic |
| TAGTGTTAATTGATTGTTGA+GGG | 0.498235 | 8.3:+70268483 | None:intergenic |
| TCTCTTGCCTTATCGAAAGA+TGG | 0.516196 | 8.3:-70268200 | MS.gene051118:CDS |
| GTAGGAAAATTCCTTTACAC+TGG | 0.545108 | 8.3:-70268557 | MS.gene051118:intron |
| GTTATTAGAGTCGTTGCTGA+AGG | 0.556540 | 8.3:+70268736 | None:intergenic |
| CCAACACAGAAGTCACATCA+TGG | 0.577575 | 8.3:-70268529 | MS.gene051118:CDS |
| CTTGTCTGAATGAACTACAA+TGG | 0.582865 | 8.3:+70268427 | None:intergenic |
| TTCATGGGACAGAACAATCA+AGG | 0.585469 | 8.3:-70268159 | MS.gene051118:CDS |
| GGTGAGAATGAATATGGGAA+AGG | 0.601558 | 8.3:+70268504 | None:intergenic |
| TTGAGGGTGAGAATGAATAT+GGG | 0.604046 | 8.3:+70268499 | None:intergenic |
| TGTTGGATGAACCAGTGTAA+AGG | 0.614019 | 8.3:+70268546 | None:intergenic |
| TTAGTGTTAATTGATTGTTG+AGG | 0.615795 | 8.3:+70268482 | None:intergenic |
| TGTGCCTTCTAATATTAACA+TGG | 0.616075 | 8.3:+70268261 | None:intergenic |
| ATCTATGAATGTTGGGAGTG+TGG | 0.617369 | 8.3:+70268313 | None:intergenic |
| AATGATGGATATGTTTACAC+CGG | 0.626851 | 8.3:-70268056 | MS.gene051118:CDS |
| GAGATGAGACTGTGCCAACA+TGG | 0.629904 | 8.3:+70268219 | None:intergenic |
| CCATGAAACAGAATATAACA+AGG | 0.635096 | 8.3:+70268175 | None:intergenic |
| TGCACATGGGTGCACCATGT+TGG | 0.643160 | 8.3:-70268233 | MS.gene051118:CDS |
| GGGACAGAACAATCAAGGTG+TGG | 0.660498 | 8.3:-70268154 | MS.gene051118:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATTATGAAATTGTTGT+GGG | + | chr8.3:70268147-70268166 | None:intergenic | 15.0% |
| !! | ATCAACAGATAAAAAAATTA+AGG | - | chr8.3:70268747-70268766 | MS.gene051118:CDS | 15.0% |
| !! | AAAACCATGTTAATATTAGA+AGG | - | chr8.3:70268515-70268534 | MS.gene051118:CDS | 20.0% |
| !! | GAAAAATTATGAAATTGTTG+TGG | + | chr8.3:70268148-70268167 | None:intergenic | 20.0% |
| !!! | AATTTTTTTATCTGTTGATC+CGG | + | chr8.3:70268746-70268765 | None:intergenic | 20.0% |
| !!! | CTAATATTAACATGGTTTTT+CGG | + | chr8.3:70268514-70268533 | None:intergenic | 20.0% |
| !!! | TAATATTAACATGGTTTTTC+GGG | + | chr8.3:70268513-70268532 | None:intergenic | 20.0% |
| ! | TAGTGTTAATTGATTGTTGA+GGG | + | chr8.3:70268300-70268319 | None:intergenic | 25.0% |
| ! | TTAGTGTTAATTGATTGTTG+AGG | + | chr8.3:70268301-70268320 | None:intergenic | 25.0% |
| !! | CTTGTTATATTCTGTTTCAT+GGG | - | chr8.3:70268606-70268625 | MS.gene051118:intron | 25.0% |
| !!! | GTGTGGTAATTAACTTTTTT+TGG | + | chr8.3:70268072-70268091 | None:intergenic | 25.0% |
| AACACAATAGTATCTGGAAA+TGG | - | chr8.3:70268319-70268338 | MS.gene051118:CDS | 30.0% | |
| AACACTAACACAATAGTATC+TGG | - | chr8.3:70268313-70268332 | MS.gene051118:CDS | 30.0% | |
| ATATCCTCTTTAACACTTGT+AGG | - | chr8.3:70268205-70268224 | MS.gene051118:CDS | 30.0% | |
| CCATGAAACAGAATATAACA+AGG | + | chr8.3:70268608-70268627 | None:intergenic | 30.0% | |
| TGAAACGATCTATGAATGTT+GGG | + | chr8.3:70268477-70268496 | None:intergenic | 30.0% | |
| TGTGCCTTCTAATATTAACA+TGG | + | chr8.3:70268522-70268541 | None:intergenic | 30.0% | |
| ! | CCTTGTTATATTCTGTTTCA+TGG | - | chr8.3:70268605-70268624 | MS.gene051118:intron | 30.0% |
| ! | TTTTCCTACAAGTGTTAAAG+AGG | + | chr8.3:70268212-70268231 | None:intergenic | 30.0% |
| !! | AATGATGGATATGTTTACAC+CGG | - | chr8.3:70268724-70268743 | MS.gene051118:CDS | 30.0% |
| !!! | TATTGTTGATTTTCCAAACG+CGG | + | chr8.3:70268408-70268427 | None:intergenic | 30.0% |
| AAGATAACAAAATCCGCGTT+TGG | - | chr8.3:70268392-70268411 | MS.gene051118:CDS | 35.0% | |
| AAGGCACAAAAAATGCACAT+GGG | - | chr8.3:70268534-70268553 | MS.gene051118:CDS | 35.0% | |
| CTTGTCTGAATGAACTACAA+TGG | + | chr8.3:70268356-70268375 | None:intergenic | 35.0% | |
| GCAATCACAGTTTCTAATGA+TGG | - | chr8.3:70268709-70268728 | MS.gene051118:intron | 35.0% | |
| GTAGGAAAATTCCTTTACAC+TGG | - | chr8.3:70268223-70268242 | MS.gene051118:CDS | 35.0% | |
| GTGAAACGATCTATGAATGT+TGG | + | chr8.3:70268478-70268497 | None:intergenic | 35.0% | |
| TGTAGAATTTCAGAGTTGTG+AGG | + | chr8.3:70268180-70268199 | None:intergenic | 35.0% | |
| TTGAGGGTGAGAATGAATAT+GGG | + | chr8.3:70268284-70268303 | None:intergenic | 35.0% | |
| AACCAAAGACTTCACGTGTT+TGG | - | chr8.3:70268651-70268670 | MS.gene051118:intron | 40.0% | |
| ATCTATGAATGTTGGGAGTG+TGG | + | chr8.3:70268470-70268489 | None:intergenic | 40.0% | |
| CAAGGTTCCATCTTTCGATA+AGG | + | chr8.3:70268590-70268609 | None:intergenic | 40.0% | |
| GGTGAGAATGAATATGGGAA+AGG | + | chr8.3:70268279-70268298 | None:intergenic | 40.0% | |
| GTTATTAGAGTCGTTGCTGA+AGG | + | chr8.3:70268047-70268066 | None:intergenic | 40.0% | |
| GTTGAGGGTGAGAATGAATA+TGG | + | chr8.3:70268285-70268304 | None:intergenic | 40.0% | |
| TCTCTTGCCTTATCGAAAGA+TGG | - | chr8.3:70268580-70268599 | MS.gene051118:intron | 40.0% | |
| TGTTGGATGAACCAGTGTAA+AGG | + | chr8.3:70268237-70268256 | None:intergenic | 40.0% | |
| TTCATGGGACAGAACAATCA+AGG | - | chr8.3:70268621-70268640 | MS.gene051118:intron | 40.0% | |
| TTCCAAACACGTGAAGTCTT+TGG | + | chr8.3:70268656-70268675 | None:intergenic | 40.0% | |
| ! | AGACTTCACGTGTTTGGAAT+CGG | - | chr8.3:70268657-70268676 | MS.gene051118:intron | 40.0% |
| ! | GAAGGCACAAAAAATGCACA+TGG | - | chr8.3:70268533-70268552 | MS.gene051118:CDS | 40.0% |
| ! | TTGTGAAGAAAGAGTACGTG+TGG | + | chr8.3:70268089-70268108 | None:intergenic | 40.0% |
| !!! | TAACATGGTTTTTCGGGATG+AGG | + | chr8.3:70268507-70268526 | None:intergenic | 40.0% |
| CCAACACAGAAGTCACATCA+TGG | - | chr8.3:70268251-70268270 | MS.gene051118:CDS | 45.0% | |
| CCATGATGTGACTTCTGTGT+TGG | + | chr8.3:70268254-70268273 | None:intergenic | 45.0% | |
| ! | ATGTTGGGAGTGTGGCTAAA+TGG | + | chr8.3:70268462-70268481 | None:intergenic | 45.0% |
| ! | TGTTGGGAGTGTGGCTAAAT+GGG | + | chr8.3:70268461-70268480 | None:intergenic | 45.0% |
| !! | TGTGGGTTTGAGTTGAGAGA+AGG | + | chr8.3:70268130-70268149 | None:intergenic | 45.0% |
| CAGAGTTGTGAGGCATGTGA+AGG | + | chr8.3:70268170-70268189 | None:intergenic | 50.0% | |
| GAGATGAGACTGTGCCAACA+TGG | + | chr8.3:70268564-70268583 | None:intergenic | 50.0% | |
| GGGACAGAACAATCAAGGTG+TGG | - | chr8.3:70268626-70268645 | MS.gene051118:intron | 50.0% | |
| TGAGAGAAGGAACTGATGCG+AGG | + | chr8.3:70268117-70268136 | None:intergenic | 50.0% | |
| TGCACATGGGTGCACCATGT+TGG | - | chr8.3:70268547-70268566 | MS.gene051118:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 70268028 | 70268774 | 70268028 | ID=MS.gene051118 |
| chr8.3 | mRNA | 70268028 | 70268774 | 70268028 | ID=MS.gene051118.t1;Parent=MS.gene051118 |
| chr8.3 | exon | 70268711 | 70268774 | 70268711 | ID=MS.gene051118.t1.exon1;Parent=MS.gene051118.t1 |
| chr8.3 | CDS | 70268711 | 70268774 | 70268711 | ID=cds.MS.gene051118.t1;Parent=MS.gene051118.t1 |
| chr8.3 | exon | 70268028 | 70268575 | 70268028 | ID=MS.gene051118.t1.exon2;Parent=MS.gene051118.t1 |
| chr8.3 | CDS | 70268028 | 70268575 | 70268028 | ID=cds.MS.gene051118.t1;Parent=MS.gene051118.t1 |
| Gene Sequence |
| Protein sequence |
