Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051677.t1 | XP_003589042.1 | 97.5 | 161 | 4 | 0 | 1 | 161 | 1 | 161 | 4.10E-85 | 323.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051677.t1 | P17093 | 90.1 | 161 | 14 | 1 | 1 | 161 | 1 | 159 | 1.7e-79 | 296.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051677.t1 | G7IBA9 | 97.5 | 161 | 4 | 0 | 1 | 161 | 1 | 161 | 3.0e-85 | 323.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051677 | MS.gene054937 | 0.815597 | 8.52E-52 | -1.69E-46 |
| MS.gene051677 | MS.gene66475 | 0.829051 | 6.39E-55 | -1.69E-46 |
| MS.gene051677 | MS.gene77282 | 0.811047 | 8.51E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051677.t1 | MTR_1g017780 | 97.516 | 161 | 4 | 0 | 1 | 161 | 1 | 161 | 5.34e-115 | 322 |
| MS.gene051677.t1 | MTR_8g076830 | 93.789 | 161 | 9 | 1 | 1 | 161 | 1 | 160 | 1.89e-98 | 280 |
| MS.gene051677.t1 | MTR_4g116410 | 93.168 | 161 | 10 | 1 | 1 | 161 | 1 | 160 | 3.69e-98 | 280 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051677.t1 | AT5G23740 | 87.578 | 161 | 18 | 1 | 1 | 161 | 1 | 159 | 9.01e-93 | 266 |
| MS.gene051677.t1 | AT3G48930 | 86.335 | 161 | 21 | 1 | 1 | 161 | 1 | 160 | 3.51e-91 | 262 |
| MS.gene051677.t1 | AT4G30800 | 86.335 | 161 | 20 | 1 | 1 | 161 | 1 | 159 | 7.99e-89 | 256 |
Find 49 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGGGGATCATGTTATTAT+TGG | 0.267190 | 1.3:-4955472 | MS.gene051677:CDS |
| ATAATTCCTCCTAACAATAA+TGG | 0.308146 | 1.3:+4955674 | None:intergenic |
| AAGCGATTGCCACCCTTTCC+AGG | 0.318925 | 1.3:+4956282 | None:intergenic |
| TGAATAAAACCATTATTGTT+AGG | 0.334061 | 1.3:-4955683 | MS.gene051677:CDS |
| GACTCCTGTAAACGCCTTCT+TGG | 0.362038 | 1.3:+4955247 | None:intergenic |
| GGTGCCAAGAAGGCGTTTAC+AGG | 0.366657 | 1.3:-4955251 | MS.gene051677:CDS |
| GGGACACTTCTTGTCAATAT+AGG | 0.373630 | 1.3:+4955767 | None:intergenic |
| TTGCTTCAGAAAAGCCTTCT+CGG | 0.396604 | 1.3:+4956350 | None:intergenic |
| TTTGAGAAAGAAACCTTCAA+TGG | 0.449940 | 1.3:+4956218 | None:intergenic |
| CCCTAGGAGTCTTAAAACTA+AGG | 0.476406 | 1.3:+4956244 | None:intergenic |
| ACTCCTAGGGAAGCCATTGA+AGG | 0.484194 | 1.3:-4956231 | MS.gene051677:intron |
| GCTGGATCTTCTAGTGGTGC+CGG | 0.487132 | 1.3:-4955272 | MS.gene051677:CDS |
| ATGTTATTATTGGCCAATGC+AGG | 0.499585 | 1.3:-4955462 | MS.gene051677:intron |
| TACAATGATCTAACCTGCAT+TGG | 0.500383 | 1.3:+4955449 | None:intergenic |
| ATCCGAGGTCGTATCATATC+CGG | 0.504599 | 1.3:-4955726 | MS.gene051677:CDS |
| TCTGGGAAGGGGAAGAGACC+TGG | 0.510724 | 1.3:-4956300 | MS.gene051677:CDS |
| AACCTCACTGTCTTAGAAAG+TGG | 0.510906 | 1.3:+4955317 | None:intergenic |
| TAGTGGTGCCGGTGCCAAGA+AGG | 0.518324 | 1.3:-4955261 | MS.gene051677:CDS |
| GACAAGAAGTGTCCCTTCAC+TGG | 0.524673 | 1.3:-4955759 | MS.gene051677:CDS |
| ATCACCTTGTTTCCGAGTGA+AGG | 0.529932 | 1.3:-4955494 | MS.gene051677:CDS |
| AAGGGGAAGAGACCTGGAAA+GGG | 0.530695 | 1.3:-4956294 | MS.gene051677:CDS |
| CCTTGTTTCCGAGTGAAGGA+AGG | 0.534218 | 1.3:-4955490 | MS.gene051677:CDS |
| CCTTCCTTCACTCGGAAACA+AGG | 0.542963 | 1.3:+4955490 | None:intergenic |
| TGCTCGAAGATATCTGGGAA+GGG | 0.550883 | 1.3:-4956312 | MS.gene051677:CDS |
| CTTGTTTCCGAGTGAAGGAA+GGG | 0.551830 | 1.3:-4955489 | MS.gene051677:CDS |
| ATAAAACCATTATTGTTAGG+AGG | 0.551945 | 1.3:-4955680 | MS.gene051677:CDS |
| GTATTGAAAGTCATCCCAGC+TGG | 0.559579 | 1.3:-4955290 | MS.gene051677:CDS |
| GGGAAGAGACCTGGAAAGGG+TGG | 0.564985 | 1.3:-4956291 | MS.gene051677:CDS |
| TTTGAATTTGTAGACCGAGA+AGG | 0.567346 | 1.3:-4956364 | MS.gene051677:intron |
| GCACCACTAGAAGATCCAGC+TGG | 0.568341 | 1.3:+4955275 | None:intergenic |
| CAAACTAACTTGTTCAGCCA+TGG | 0.568984 | 1.3:+4956463 | None:intergenic |
| ATCCCAGCTGGATCTTCTAG+TGG | 0.570183 | 1.3:-4955278 | MS.gene051677:CDS |
| GAAGGGGAAGAGACCTGGAA+AGG | 0.571357 | 1.3:-4956295 | MS.gene051677:CDS |
| CGTGTGCAGGAATATTAGAA+TGG | 0.572181 | 1.3:+4955518 | None:intergenic |
| CTTAGCACTATGGCAAGTAC+CGG | 0.576116 | 1.3:+4955707 | None:intergenic |
| CATGATCCCCTTCCTTCACT+CGG | 0.580378 | 1.3:+4955482 | None:intergenic |
| GTGCTCGAAGATATCTGGGA+AGG | 0.582495 | 1.3:-4956313 | MS.gene051677:CDS |
| AAACCTTCAATGGCTTCCCT+AGG | 0.582868 | 1.3:+4956228 | None:intergenic |
| AAACAAGGTGATACGTGTGC+AGG | 0.596227 | 1.3:+4955505 | None:intergenic |
| GCTCGAAGATATCTGGGAAG+GGG | 0.598893 | 1.3:-4956311 | MS.gene051677:CDS |
| GATTGAGACATTACCAGTGA+AGG | 0.618985 | 1.3:+4955746 | None:intergenic |
| TTGTTTCCGAGTGAAGGAAG+GGG | 0.622320 | 1.3:-4955488 | MS.gene051677:CDS |
| ATTGAGACATTACCAGTGAA+GGG | 0.624989 | 1.3:+4955747 | None:intergenic |
| CACCACTAGAAGATCCAGCT+GGG | 0.629304 | 1.3:+4955276 | None:intergenic |
| ACTGGTAATGTCTCAATCCG+AGG | 0.640857 | 1.3:-4955741 | MS.gene051677:CDS |
| TGTAAACGCCTTCTTGGCAC+CGG | 0.648607 | 1.3:+4955253 | None:intergenic |
| ATTTCATTAAGAAATATCAG+AGG | 0.666848 | 1.3:-4955647 | MS.gene051677:intron |
| GGCCACTTTCTAAGACAGTG+AGG | 0.709841 | 1.3:-4955319 | MS.gene051677:CDS |
| TACCGGATATGATACGACCT+CGG | 0.712271 | 1.3:+4955724 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTGTTTTGATAAATCT+TGG | - | chr1.3:4955293-4955312 | MS.gene051677:CDS | 15.0% |
| !! | ATTTCATTAAGAAATATCAG+AGG | - | chr1.3:4956058-4956077 | MS.gene051677:intron | 20.0% |
| !! | CAAATTATAGATTAATGTGA+AGG | + | chr1.3:4955271-4955290 | None:intergenic | 20.0% |
| !! | GTATTAGATTACAAAAAGAA+AGG | + | chr1.3:4955896-4955915 | None:intergenic | 20.0% |
| !! | GTTACCAATTTATTTACATA+AGG | - | chr1.3:4955869-4955888 | MS.gene051677:intron | 20.0% |
| !! | TGAATAAAACCATTATTGTT+AGG | - | chr1.3:4956022-4956041 | MS.gene051677:intron | 20.0% |
| !! | TTCCTATAAGATTAAGAAAA+AGG | + | chr1.3:4955918-4955937 | None:intergenic | 20.0% |
| !!! | TACCTTTTTCTTAATCTTAT+AGG | - | chr1.3:4955913-4955932 | MS.gene051677:intron | 20.0% |
| !!! | TAGATTGGTTTATTATTGAT+AGG | - | chr1.3:4955602-4955621 | MS.gene051677:intron | 20.0% |
| ! | AAGCTTATGAAAATAGCTTA+TGG | + | chr1.3:4955710-4955729 | None:intergenic | 25.0% |
| ! | AGGACCTTATGTAAATAAAT+TGG | + | chr1.3:4955876-4955895 | None:intergenic | 25.0% |
| ! | AGGACTTATTAAAACAGTTT+AGG | + | chr1.3:4955780-4955799 | None:intergenic | 25.0% |
| ! | ATAAAACCATTATTGTTAGG+AGG | - | chr1.3:4956025-4956044 | MS.gene051677:intron | 25.0% |
| ! | ATAATTCCTCCTAACAATAA+TGG | + | chr1.3:4956034-4956053 | None:intergenic | 25.0% |
| ! | TAAACCACGAATATGAAAAT+TGG | + | chr1.3:4955519-4955538 | None:intergenic | 25.0% |
| ! | TCTTGGATTTACTAACTAAT+TGG | - | chr1.3:4955310-4955329 | MS.gene051677:CDS | 25.0% |
| ! | TTTAAGAATCATAGGTAGAT+TGG | - | chr1.3:4955587-4955606 | MS.gene051677:intron | 25.0% |
| !! | TTTTATTCATCTTAGCACTA+TGG | + | chr1.3:4956011-4956030 | None:intergenic | 25.0% |
| !! | TTTTCATATTCGTGGTTTAA+TGG | - | chr1.3:4955520-4955539 | MS.gene051677:CDS | 25.0% |
| !!! | AATCGCTTTTATAAATCTGT+TGG | - | chr1.3:4955438-4955457 | MS.gene051677:intron | 25.0% |
| !!! | CATTGTGTTTTAAGAATCAT+AGG | - | chr1.3:4955579-4955598 | MS.gene051677:intron | 25.0% |
| !!! | TTGTTTTGTTTATCCAAGTA+AGG | - | chr1.3:4956310-4956329 | MS.gene051677:CDS | 25.0% |
| CTCTGTTTGGTAAAAAATAG+TGG | + | chr1.3:4955687-4955706 | None:intergenic | 30.0% | |
| TGTGTATGCTTGTTCTTATT+AGG | - | chr1.3:4956153-4956172 | MS.gene051677:intron | 30.0% | |
| TTTGAGAAAGAAACCTTCAA+TGG | + | chr1.3:4955490-4955509 | None:intergenic | 30.0% | |
| ! | GTTTCCAATTTTCATATTCG+TGG | - | chr1.3:4955512-4955531 | MS.gene051677:CDS | 30.0% |
| AAGCTTTCATGAAGAGCTTA+TGG | - | chr1.3:4955724-4955743 | MS.gene051677:CDS | 35.0% | |
| ACAAATAGTTAACACACCTG+TGG | + | chr1.3:4956133-4956152 | None:intergenic | 35.0% | |
| AGATAAGCTCAAATAAGCCA+TGG | - | chr1.3:4955837-4955856 | MS.gene051677:intron | 35.0% | |
| AGCAGAAGTAAAGGTAATCA+AGG | + | chr1.3:4956358-4956377 | None:intergenic | 35.0% | |
| ATGTTATTATTGGCCAATGC+AGG | - | chr1.3:4956243-4956262 | MS.gene051677:CDS | 35.0% | |
| ATTGAGACATTACCAGTGAA+GGG | + | chr1.3:4955961-4955980 | None:intergenic | 35.0% | |
| CACAAAAACAGTTCCTTACT+TGG | + | chr1.3:4956326-4956345 | None:intergenic | 35.0% | |
| CTATGAACAAGCAGAAGTAA+AGG | + | chr1.3:4956367-4956386 | None:intergenic | 35.0% | |
| CTTTACTTCTGCTTGTTCAT+AGG | - | chr1.3:4956365-4956384 | MS.gene051677:intron | 35.0% | |
| GAAGGGGATCATGTTATTAT+TGG | - | chr1.3:4956233-4956252 | MS.gene051677:CDS | 35.0% | |
| GCAGAAGTAAAGGTAATCAA+GGG | + | chr1.3:4956357-4956376 | None:intergenic | 35.0% | |
| TACAATGATCTAACCTGCAT+TGG | + | chr1.3:4956259-4956278 | None:intergenic | 35.0% | |
| TTACTGTATTTGTATGCCAC+AGG | - | chr1.3:4956114-4956133 | MS.gene051677:intron | 35.0% | |
| TTTGAATTTGTAGACCGAGA+AGG | - | chr1.3:4955341-4955360 | MS.gene051677:intron | 35.0% | |
| ! | AAATAGCTTATGGCTCTGTT+TGG | + | chr1.3:4955700-4955719 | None:intergenic | 35.0% |
| ! | TTGGTAACATAAACATGCCA+TGG | + | chr1.3:4955857-4955876 | None:intergenic | 35.0% |
| ! | TTTTGTGCTCGAAGATATCT+GGG | - | chr1.3:4955388-4955407 | MS.gene051677:intron | 35.0% |
| ! | TTTTTGTGCTCGAAGATATC+TGG | - | chr1.3:4955387-4955406 | MS.gene051677:intron | 35.0% |
| !!! | CCTTAGTTTTAAGACTCCTA+GGG | - | chr1.3:4955461-4955480 | MS.gene051677:intron | 35.0% |
| AACCTCACTGTCTTAGAAAG+TGG | + | chr1.3:4956391-4956410 | None:intergenic | 40.0% | |
| ATTCATTCCCCTTTCTTACC+AGG | - | chr1.3:4956082-4956101 | MS.gene051677:intron | 40.0% | |
| CCCTAGGAGTCTTAAAACTA+AGG | + | chr1.3:4955464-4955483 | None:intergenic | 40.0% | |
| CGTGTGCAGGAATATTAGAA+TGG | + | chr1.3:4956190-4956209 | None:intergenic | 40.0% | |
| GATTGAGACATTACCAGTGA+AGG | + | chr1.3:4955962-4955981 | None:intergenic | 40.0% | |
| GGGACACTTCTTGTCAATAT+AGG | + | chr1.3:4955941-4955960 | None:intergenic | 40.0% | |
| TTCATTCCCCTTTCTTACCA+GGG | - | chr1.3:4956083-4956102 | MS.gene051677:intron | 40.0% | |
| TTGCTTCAGAAAAGCCTTCT+CGG | + | chr1.3:4955358-4955377 | None:intergenic | 40.0% | |
| !!! | GCCTTAGTTTTAAGACTCCT+AGG | - | chr1.3:4955460-4955479 | MS.gene051677:intron | 40.0% |
| AAACAAGGTGATACGTGTGC+AGG | + | chr1.3:4956203-4956222 | None:intergenic | 45.0% | |
| AAACCTTCAATGGCTTCCCT+AGG | + | chr1.3:4955480-4955499 | None:intergenic | 45.0% | |
| ATCCGAGGTCGTATCATATC+CGG | - | chr1.3:4955979-4955998 | MS.gene051677:intron | 45.0% | |
| CTTAGCACTATGGCAAGTAC+CGG | + | chr1.3:4956001-4956020 | None:intergenic | 45.0% | |
| CTTGTTTCCGAGTGAAGGAA+GGG | - | chr1.3:4956216-4956235 | MS.gene051677:intron | 45.0% | |
| GTATTGAAAGTCATCCCAGC+TGG | - | chr1.3:4956415-4956434 | MS.gene051677:intron | 45.0% | |
| TACCGGATATGATACGACCT+CGG | + | chr1.3:4955984-4956003 | None:intergenic | 45.0% | |
| TGCTCGAAGATATCTGGGAA+GGG | - | chr1.3:4955393-4955412 | MS.gene051677:intron | 45.0% | |
| TTGTTTCCGAGTGAAGGAAG+GGG | - | chr1.3:4956217-4956236 | MS.gene051677:intron | 45.0% | |
| ! | ACTGGTAATGTCTCAATCCG+AGG | - | chr1.3:4955964-4955983 | MS.gene051677:intron | 45.0% |
| ! | ATCACCTTGTTTCCGAGTGA+AGG | - | chr1.3:4956211-4956230 | MS.gene051677:intron | 45.0% |
| AAGGGGAAGAGACCTGGAAA+GGG | - | chr1.3:4955411-4955430 | MS.gene051677:intron | 50.0% | |
| ACTCCTAGGGAAGCCATTGA+AGG | - | chr1.3:4955474-4955493 | MS.gene051677:CDS | 50.0% | |
| ATACAGTAAACTGCGCACCC+TGG | + | chr1.3:4956103-4956122 | None:intergenic | 50.0% | |
| ATCCCAGCTGGATCTTCTAG+TGG | - | chr1.3:4956427-4956446 | MS.gene051677:intron | 50.0% | |
| CACCACTAGAAGATCCAGCT+GGG | + | chr1.3:4956432-4956451 | None:intergenic | 50.0% | |
| CATGATCCCCTTCCTTCACT+CGG | + | chr1.3:4956226-4956245 | None:intergenic | 50.0% | |
| CCTTCCTTCACTCGGAAACA+AGG | + | chr1.3:4956218-4956237 | None:intergenic | 50.0% | |
| GACTCCTGTAAACGCCTTCT+TGG | + | chr1.3:4956461-4956480 | None:intergenic | 50.0% | |
| GCTCGAAGATATCTGGGAAG+GGG | - | chr1.3:4955394-4955413 | MS.gene051677:intron | 50.0% | |
| GTGACATCATTTGCGTTGCG+AGG | + | chr1.3:4955800-4955819 | None:intergenic | 50.0% | |
| GTGCTCGAAGATATCTGGGA+AGG | - | chr1.3:4955392-4955411 | MS.gene051677:intron | 50.0% | |
| TGTAAACGCCTTCTTGGCAC+CGG | + | chr1.3:4956455-4956474 | None:intergenic | 50.0% | |
| ! | CCTTGTTTCCGAGTGAAGGA+AGG | - | chr1.3:4956215-4956234 | MS.gene051677:intron | 50.0% |
| !! | GACAAGAAGTGTCCCTTCAC+TGG | - | chr1.3:4955946-4955965 | MS.gene051677:intron | 50.0% |
| !! | GGCCACTTTCTAAGACAGTG+AGG | - | chr1.3:4956386-4956405 | MS.gene051677:intron | 50.0% |
| AAGCGATTGCCACCCTTTCC+AGG | + | chr1.3:4955426-4955445 | None:intergenic | 55.0% | |
| ACTGCGCACCCTGGTAAGAA+AGG | + | chr1.3:4956094-4956113 | None:intergenic | 55.0% | |
| CTGCGCACCCTGGTAAGAAA+GGG | + | chr1.3:4956093-4956112 | None:intergenic | 55.0% | |
| GAAGGGGAAGAGACCTGGAA+AGG | - | chr1.3:4955410-4955429 | MS.gene051677:intron | 55.0% | |
| GCACCACTAGAAGATCCAGC+TGG | + | chr1.3:4956433-4956452 | None:intergenic | 55.0% | |
| GCTGGATCTTCTAGTGGTGC+CGG | - | chr1.3:4956433-4956452 | MS.gene051677:intron | 55.0% | |
| TGCGCACCCTGGTAAGAAAG+GGG | + | chr1.3:4956092-4956111 | None:intergenic | 55.0% | |
| !! | GGTGCCAAGAAGGCGTTTAC+AGG | - | chr1.3:4956454-4956473 | MS.gene051677:intron | 55.0% |
| GGGAAGAGACCTGGAAAGGG+TGG | - | chr1.3:4955414-4955433 | MS.gene051677:intron | 60.0% | |
| TCTGGGAAGGGGAAGAGACC+TGG | - | chr1.3:4955405-4955424 | MS.gene051677:intron | 60.0% | |
| !! | TAGTGGTGCCGGTGCCAAGA+AGG | - | chr1.3:4956444-4956463 | MS.gene051677:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 4955244 | 4956483 | 4955244 | ID=MS.gene051677 |
| chr1.3 | mRNA | 4955244 | 4956483 | 4955244 | ID=MS.gene051677.t1;Parent=MS.gene051677 |
| chr1.3 | exon | 4956472 | 4956483 | 4956472 | ID=MS.gene051677.t1.exon1;Parent=MS.gene051677.t1 |
| chr1.3 | CDS | 4956472 | 4956483 | 4956472 | ID=cds.MS.gene051677.t1;Parent=MS.gene051677.t1 |
| chr1.3 | exon | 4956232 | 4956373 | 4956232 | ID=MS.gene051677.t1.exon2;Parent=MS.gene051677.t1 |
| chr1.3 | CDS | 4956232 | 4956373 | 4956232 | ID=cds.MS.gene051677.t1;Parent=MS.gene051677.t1 |
| chr1.3 | exon | 4955648 | 4955792 | 4955648 | ID=MS.gene051677.t1.exon3;Parent=MS.gene051677.t1 |
| chr1.3 | CDS | 4955648 | 4955792 | 4955648 | ID=cds.MS.gene051677.t1;Parent=MS.gene051677.t1 |
| chr1.3 | exon | 4955463 | 4955552 | 4955463 | ID=MS.gene051677.t1.exon4;Parent=MS.gene051677.t1 |
| chr1.3 | CDS | 4955463 | 4955552 | 4955463 | ID=cds.MS.gene051677.t1;Parent=MS.gene051677.t1 |
| chr1.3 | exon | 4955244 | 4955340 | 4955244 | ID=MS.gene051677.t1.exon5;Parent=MS.gene051677.t1 |
| chr1.3 | CDS | 4955244 | 4955340 | 4955244 | ID=cds.MS.gene051677.t1;Parent=MS.gene051677.t1 |
| Gene Sequence |
| Protein sequence |
