AlfalfaGEDB | MS.gene051741

M. sativa cultivar XinJiangDaYe / MS.gene051741

KEGG: K02935
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00542.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051741.t1	XP_013448534.1	90.8	218	19	1	1	218	1	217	2.80E-92	348.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051741.t1	P53163	36.5	167	96	4	62	218	28	194	2.1e-16	87.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051741.t1	I3SW10	90.8	218	19	1	1	218	1	217	2.0e-92	348.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene005792	MS.gene051741	PPI
MS.gene60517	MS.gene051741	PPI
MS.gene68085	MS.gene051741	PPI
MS.gene051741	MS.gene09988	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051741.t1	MTR_7g053520	94.954	218	10	1	1	218	1	217	1.09e-141	394
MS.gene051741.t1	MTR_2g064300	84.862	218	31	2	1	218	1	216	1.69e-121	343
MS.gene051741.t1	MTR_2g031760	41.212	165	92	3	55	218	30	190	1.83e-35	124
MS.gene051741.t1	MTR_8g076780	47.973	148	67	2	80	218	49	195	1.39e-31	114
MS.gene051741.t1	MTR_2g031960	48.120	133	64	2	87	218	27	155	1.79e-29	107

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051741.t1	AT1G70190	55.856	222	80	4	1	218	1	208	3.66e-67	206
MS.gene051741.t1	AT1G70190	55.856	222	80	4	1	218	1	208	3.66e-67	206
MS.gene051741.t1	AT4G36420	43.455	191	92	5	31	218	2	179	3.36e-34	120
MS.gene051741.t1	AT3G06040	44.667	150	74	3	70	218	45	186	3.82e-31	113
MS.gene051741.t1	AT3G06040	44.667	150	74	3	70	218	45	186	3.82e-31	113
MS.gene051741.t1	AT3G06040	44.667	150	74	3	70	218	45	186	3.82e-31	113
MS.gene051741.t1	AT4G37660	47.727	132	61	2	87	218	44	167	8.96e-26	98.6
MS.gene051741.t1	AT2G03130	55.294	85	38	0	134	218	7	91	5.69e-16	71.2

Find 49 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTGATGACCTTAATTT+TGG	0.127344	7.3:-50427655	None:intergenic
GAAACCAGGATCTGTAAATT+TGG	0.208162	7.3:+50427536	MS.gene051741:CDS
ACACCTTTCTTTATAATAGA+AGG	0.301571	7.3:-50427735	None:intergenic
TGAAGCAGCTTCCAAAATTA+AGG	0.341436	7.3:+50427644	MS.gene051741:CDS
TCTAGCTGAAGGTGCAGAAT+TGG	0.346308	7.3:+50427458	MS.gene051741:CDS
TTGTCTATACAACAAACTAT+TGG	0.364579	7.3:-50427205	None:intergenic
CCAGCCAAATTTACAGATCC+TGG	0.386608	7.3:-50427540	None:intergenic
GAAGGAGGCAAAGGATTTAG+TGG	0.417133	7.3:+50427704	MS.gene051741:CDS
TGTCTATACAACAAACTATT+GGG	0.417171	7.3:-50427204	None:intergenic
CTTTCCTTGCAGCTTGACCT+AGG	0.449900	7.3:-50427287	None:intergenic
CCAGGATCTGTAAATTTGGC+TGG	0.453905	7.3:+50427540	MS.gene051741:CDS
CCTCTTACTGCTAGCACTGT+AGG	0.457292	7.3:+50427228	MS.gene051741:CDS
CTAGCTGAAGGTGCAGAATT+GGG	0.458679	7.3:+50427459	MS.gene051741:CDS
ATCTGTAAATTTGGCTGGAA+TGG	0.461697	7.3:+50427545	MS.gene051741:CDS
CCTACAGTGCTAGCAGTAAG+AGG	0.466523	7.3:-50427228	None:intergenic
TCTAGGCGCAATTGTCGTTA+TGG	0.475224	7.3:+50427800	MS.gene051741:CDS
TCAGTAGGATCAAATGTTGC+AGG	0.479265	7.3:-50427375	None:intergenic
AAAGGTGTTTCGAAGGAAGA+AGG	0.479617	7.3:+50427750	MS.gene051741:CDS
GGTTTCATAAATCCCACATT+GGG	0.482477	7.3:-50427519	None:intergenic
AGCTGCAAGGAAAGAGGAGG+AGG	0.499403	7.3:+50427296	MS.gene051741:CDS
TGCAAGGAAAGAGGAGGAGG+AGG	0.500493	7.3:+50427299	MS.gene051741:CDS
ACACCTTCTATTATAAAGAA+AGG	0.509780	7.3:+50427732	MS.gene051741:CDS
GTTGCAGGATCAAAATCAGT+TGG	0.513514	7.3:-50427360	None:intergenic
CTGAAAACTCTTTCCGATGG+AGG	0.526100	7.3:-50427408	None:intergenic
TTCCAAAATTAAGGTCATCA+AGG	0.526956	7.3:+50427653	MS.gene051741:CDS
TTTCAGGCTTGTTGATGAAG+TGG	0.533852	7.3:+50427425	MS.gene051741:CDS
TATAAAGAAAGGTGTTTCGA+AGG	0.534435	7.3:+50427743	MS.gene051741:CDS
AAGGTCATCAAGGAGGTTCG+AGG	0.535857	7.3:+50427663	MS.gene051741:CDS
CTCTTACTGCTAGCACTGTA+GGG	0.537187	7.3:+50427229	MS.gene051741:CDS
TGATCATCGTGGGCCTCCAT+CGG	0.539706	7.3:+50427395	MS.gene051741:CDS
ATAGAGAAATTGAAAGCTCT+AGG	0.542277	7.3:+50427783	MS.gene051741:CDS
TTTAGGTTTGAAGGAGGCAA+AGG	0.553743	7.3:+50427695	MS.gene051741:CDS
AGGTCAAGCTGCAAGGAAAG+AGG	0.555154	7.3:+50427290	MS.gene051741:CDS
TTGCAGGATCAAAATCAGTT+GGG	0.555773	7.3:-50427359	None:intergenic
GATGTATTTACAAGAAACCT+AGG	0.571651	7.3:+50427270	MS.gene051741:CDS
AGCCTGAAAACTCTTTCCGA+TGG	0.583898	7.3:-50427411	None:intergenic
TGGTTTCATAAATCCCACAT+TGG	0.595026	7.3:-50427520	None:intergenic
GGAGGCCCACGATGATCAGT+AGG	0.597332	7.3:-50427390	None:intergenic
TACTGATTTAGGTTTGAAGG+AGG	0.609454	7.3:+50427689	MS.gene051741:CDS
GAAACCTAGGTCAAGCTGCA+AGG	0.610947	7.3:+50427283	MS.gene051741:CDS
CAAAATTAAGGTCATCAAGG+AGG	0.615684	7.3:+50427656	MS.gene051741:CDS
AACATCAGCAGAAGCCAAGG+AGG	0.619521	7.3:+50427575	MS.gene051741:CDS
GCATCTCTAACTCTAGCTGA+AGG	0.625143	7.3:+50427447	MS.gene051741:CDS
TCAAGCTGCAAGGAAAGAGG+AGG	0.631410	7.3:+50427293	MS.gene051741:CDS
AATGTGGGATTTATGAAACC+AGG	0.639785	7.3:+50427522	MS.gene051741:CDS
GATAAAGGAGATGCCCAATG+TGG	0.647613	7.3:+50427506	MS.gene051741:CDS
ATAAAGGAGATGCCCAATGT+GGG	0.651305	7.3:+50427507	MS.gene051741:CDS
TTGATCCTACTGATCATCGT+GGG	0.654861	7.3:+50427385	MS.gene051741:CDS
TTTGATCCTACTGATCATCG+TGG	0.702613	7.3:+50427384	MS.gene051741:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTCAAATACAGTTTTTTC+TGG	-	chr7.3:50427609-50427628	None:intergenic	20.0%
!	ACACCTTCTATTATAAAGAA+AGG	+	chr7.3:50427732-50427751	MS.gene051741:CDS	25.0%
!	ACACCTTTCTTTATAATAGA+AGG	-	chr7.3:50427738-50427757	None:intergenic	25.0%
!	TGTCTATACAACAAACTATT+GGG	-	chr7.3:50427207-50427226	None:intergenic	25.0%
!	TTGTCTATACAACAAACTAT+TGG	-	chr7.3:50427208-50427227	None:intergenic	25.0%
!!!	TGAGTTTGCTTTTAAGAATA+AGG	+	chr7.3:50427172-50427191	MS.gene051741:CDS	25.0%
!!!	TTTTACTGATTTAGGTTTGA+AGG	+	chr7.3:50427686-50427705	MS.gene051741:CDS	25.0%
	ATAGAGAAATTGAAAGCTCT+AGG	+	chr7.3:50427783-50427802	MS.gene051741:CDS	30.0%
	GATGAAAAAAATGGGGATAA+AGG	+	chr7.3:50427491-50427510	MS.gene051741:CDS	30.0%
	GATGTATTTACAAGAAACCT+AGG	+	chr7.3:50427270-50427289	MS.gene051741:CDS	30.0%
	TTCCAAAATTAAGGTCATCA+AGG	+	chr7.3:50427653-50427672	MS.gene051741:CDS	30.0%
!!	TATAAAGAAAGGTGTTTCGA+AGG	+	chr7.3:50427743-50427762	MS.gene051741:CDS	30.0%
	AATGTGGGATTTATGAAACC+AGG	+	chr7.3:50427522-50427541	MS.gene051741:CDS	35.0%
	ATCTGTAAATTTGGCTGGAA+TGG	+	chr7.3:50427545-50427564	MS.gene051741:CDS	35.0%
	CAAAATTAAGGTCATCAAGG+AGG	+	chr7.3:50427656-50427675	MS.gene051741:CDS	35.0%
	CTCGCTCTGATGAAAAAAAT+GGG	+	chr7.3:50427483-50427502	MS.gene051741:CDS	35.0%
	GAAACCAGGATCTGTAAATT+TGG	+	chr7.3:50427536-50427555	MS.gene051741:CDS	35.0%
	GGTTTCATAAATCCCACATT+GGG	-	chr7.3:50427522-50427541	None:intergenic	35.0%
	TCGCTCTGATGAAAAAAATG+GGG	+	chr7.3:50427484-50427503	MS.gene051741:CDS	35.0%
	TCTCGCTCTGATGAAAAAAA+TGG	+	chr7.3:50427482-50427501	MS.gene051741:CDS	35.0%
	TGAAGCAGCTTCCAAAATTA+AGG	+	chr7.3:50427644-50427663	MS.gene051741:CDS	35.0%
	TGGTTTCATAAATCCCACAT+TGG	-	chr7.3:50427523-50427542	None:intergenic	35.0%
	TTGCAGGATCAAAATCAGTT+GGG	-	chr7.3:50427362-50427381	None:intergenic	35.0%
!!	AATTTTGGAAGCTGCTTCAT+AGG	-	chr7.3:50427643-50427662	None:intergenic	35.0%
!!	CTCCTTGATGACCTTAATTT+TGG	-	chr7.3:50427658-50427677	None:intergenic	35.0%
!!	TACTGATTTAGGTTTGAAGG+AGG	+	chr7.3:50427689-50427708	MS.gene051741:CDS	35.0%
!!!	GTTCGAGGTTTTACTGATTT+AGG	+	chr7.3:50427678-50427697	MS.gene051741:CDS	35.0%
	AAAAACATCAGCAGAAGCCA+AGG	+	chr7.3:50427572-50427591	MS.gene051741:CDS	40.0%
	ATAAAGGAGATGCCCAATGT+GGG	+	chr7.3:50427507-50427526	MS.gene051741:CDS	40.0%
	GTTGCAGGATCAAAATCAGT+TGG	-	chr7.3:50427363-50427382	None:intergenic	40.0%
	TCAGTAGGATCAAATGTTGC+AGG	-	chr7.3:50427378-50427397	None:intergenic	40.0%
	TTGATCCTACTGATCATCGT+GGG	+	chr7.3:50427385-50427404	MS.gene051741:CDS	40.0%
	TTTGATCCTACTGATCATCG+TGG	+	chr7.3:50427384-50427403	MS.gene051741:CDS	40.0%
!	TTTAGGTTTGAAGGAGGCAA+AGG	+	chr7.3:50427695-50427714	MS.gene051741:CDS	40.0%
!!	AAAGGTGTTTCGAAGGAAGA+AGG	+	chr7.3:50427750-50427769	MS.gene051741:CDS	40.0%
!!	TTTCAGGCTTGTTGATGAAG+TGG	+	chr7.3:50427425-50427444	MS.gene051741:CDS	40.0%
	AGCCTGAAAACTCTTTCCGA+TGG	-	chr7.3:50427414-50427433	None:intergenic	45.0%
	CCAGCCAAATTTACAGATCC+TGG	-	chr7.3:50427543-50427562	None:intergenic	45.0%
	CCAGGATCTGTAAATTTGGC+TGG	+	chr7.3:50427540-50427559	MS.gene051741:CDS	45.0%
	CTCTTACTGCTAGCACTGTA+GGG	+	chr7.3:50427229-50427248	MS.gene051741:CDS	45.0%
	CTGAAAACTCTTTCCGATGG+AGG	-	chr7.3:50427411-50427430	None:intergenic	45.0%
	GAAGGAGGCAAAGGATTTAG+TGG	+	chr7.3:50427704-50427723	MS.gene051741:CDS	45.0%
	GATAAAGGAGATGCCCAATG+TGG	+	chr7.3:50427506-50427525	MS.gene051741:CDS	45.0%
	GCATCTCTAACTCTAGCTGA+AGG	+	chr7.3:50427447-50427466	MS.gene051741:CDS	45.0%
	TCTAGGCGCAATTGTCGTTA+TGG	+	chr7.3:50427800-50427819	MS.gene051741:CDS	45.0%
!	CTAGCTGAAGGTGCAGAATT+GGG	+	chr7.3:50427459-50427478	MS.gene051741:CDS	45.0%
!	CTCCATCGGAAAGAGTTTTC+AGG	+	chr7.3:50427409-50427428	MS.gene051741:CDS	45.0%
!	TCTAGCTGAAGGTGCAGAAT+TGG	+	chr7.3:50427458-50427477	MS.gene051741:CDS	45.0%
	AACATCAGCAGAAGCCAAGG+AGG	+	chr7.3:50427575-50427594	MS.gene051741:CDS	50.0%
	AAGGTCATCAAGGAGGTTCG+AGG	+	chr7.3:50427663-50427682	MS.gene051741:CDS	50.0%
	AGGTCAAGCTGCAAGGAAAG+AGG	+	chr7.3:50427290-50427309	MS.gene051741:CDS	50.0%
	CCTACAGTGCTAGCAGTAAG+AGG	-	chr7.3:50427231-50427250	None:intergenic	50.0%
	CCTCTTACTGCTAGCACTGT+AGG	+	chr7.3:50427228-50427247	MS.gene051741:CDS	50.0%
	CTTTCCTTGCAGCTTGACCT+AGG	-	chr7.3:50427290-50427309	None:intergenic	50.0%
	GAAACCTAGGTCAAGCTGCA+AGG	+	chr7.3:50427283-50427302	MS.gene051741:CDS	50.0%
	TCAAGCTGCAAGGAAAGAGG+AGG	+	chr7.3:50427293-50427312	MS.gene051741:CDS	50.0%
!!!	TTCTGGCTTTTGCTCCTCCT+TGG	-	chr7.3:50427592-50427611	None:intergenic	50.0%
	AGCTGCAAGGAAAGAGGAGG+AGG	+	chr7.3:50427296-50427315	MS.gene051741:CDS	55.0%
	TGATCATCGTGGGCCTCCAT+CGG	+	chr7.3:50427395-50427414	MS.gene051741:CDS	55.0%
	TGCAAGGAAAGAGGAGGAGG+AGG	+	chr7.3:50427299-50427318	MS.gene051741:CDS	55.0%
	GGAGGCCCACGATGATCAGT+AGG	-	chr7.3:50427393-50427412	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	50427171	50427827	50427171	ID=MS.gene051741
chr7.3	mRNA	50427171	50427827	50427171	ID=MS.gene051741.t1;Parent=MS.gene051741
chr7.3	exon	50427171	50427827	50427171	ID=MS.gene051741.t1.exon1;Parent=MS.gene051741.t1
chr7.3	CDS	50427171	50427827	50427171	ID=cds.MS.gene051741.t1;Parent=MS.gene051741.t1

Gene Sequence

>MS.gene051741

ATGAGTTTGCTTTTAAGAATAAGGCATTGTTTACCCAATAGTTTGTTGTATAGACAACCTCTTACTGCTAGCACTGTAGGGCTTAATGCTAGAACATTAGATGTATTTACAAGAAACCTAGGTCAAGCTGCAAGGAAAGAGGAGGAGGAGGAAGATGTAGAAGAAGTTGAAATTGATCAACGAAGTCTCCCAACTGATTTTGATCCTGCAACATTTGATCCTACTGATCATCGTGGGCCTCCATCGGAAAGAGTTTTCAGGCTTGTTGATGAAGTGGCATCTCTAACTCTAGCTGAAGGTGCAGAATTGGGTCTCGCTCTGATGAAAAAAATGGGGATAAAGGAGATGCCCAATGTGGGATTTATGAAACCAGGATCTGTAAATTTGGCTGGAATGGCAGCAAAAACATCAGCAGAAGCCAAGGAGGAGCAAAAGCCAGAAAAAACTGTATTTGAATTGAAACTGACGTCCTATGAAGCAGCTTCCAAAATTAAGGTCATCAAGGAGGTTCGAGGTTTTACTGATTTAGGTTTGAAGGAGGCAAAGGATTTAGTGGAAAAAACACCTTCTATTATAAAGAAAGGTGTTTCGAAGGAAGAAGGAGAGCAGATAATAGAGAAATTGAAAGCTCTAGGCGCAATTGTCGTTATGGAATGA

Protein sequence

>MS.gene051741.t1

MSLLLRIRHCLPNSLLYRQPLTASTVGLNARTLDVFTRNLGQAARKEEEEEDVEEVEIDQRSLPTDFDPATFDPTDHRGPPSERVFRLVDEVASLTLAEGAELGLALMKKMGIKEMPNVGFMKPGSVNLAGMAAKTSAEAKEEQKPEKTVFELKLTSYEAASKIKVIKEVRGFTDLGLKEAKDLVEKTPSIIKKGVSKEEGEQIIEKLKALGAIVVME