Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051760.t1 | XP_003625838.1 | 97.5 | 80 | 2 | 0 | 1 | 80 | 133 | 212 | 7.40E-35 | 156 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051760.t1 | Q9ASP6 | 59.2 | 76 | 31 | 0 | 5 | 80 | 155 | 230 | 1.0e-21 | 103.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051760.t1 | G7KQW4 | 97.5 | 80 | 2 | 0 | 1 | 80 | 133 | 212 | 5.3e-35 | 156.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051760 | MS.gene051800 | 0.80017 | 1.63E-48 | -1.69E-46 |
| MS.gene051760 | MS.gene052514 | 0.825368 | 4.87E-54 | -1.69E-46 |
| MS.gene051760 | MS.gene052609 | 0.812187 | 4.81E-51 | -1.69E-46 |
| MS.gene051760 | MS.gene055467 | 0.805322 | 1.41E-49 | -1.69E-46 |
| MS.gene051760 | MS.gene056308 | 0.80071 | 1.27E-48 | -1.69E-46 |
| MS.gene051760 | MS.gene056507 | 0.840345 | 9.20E-58 | -1.69E-46 |
| MS.gene051760 | MS.gene058010 | 0.804604 | 1.99E-49 | -1.69E-46 |
| MS.gene051760 | MS.gene058919 | 0.802006 | 6.88E-49 | -1.69E-46 |
| MS.gene051760 | MS.gene06133 | 0.801766 | 7.71E-49 | -1.69E-46 |
| MS.gene051760 | MS.gene23030 | 0.834468 | 2.95E-56 | -1.69E-46 |
| MS.gene051760 | MS.gene27302 | 0.815932 | 7.17E-52 | -1.69E-46 |
| MS.gene051760 | MS.gene32028 | 0.824765 | 6.76E-54 | -1.69E-46 |
| MS.gene051760 | MS.gene43161 | 0.807105 | 5.95E-50 | -1.69E-46 |
| MS.gene051760 | MS.gene44969 | 0.80523 | 1.48E-49 | -1.69E-46 |
| MS.gene051760 | MS.gene49422 | 0.823071 | 1.69E-53 | -1.69E-46 |
| MS.gene051760 | MS.gene49423 | 0.831284 | 1.82E-55 | -1.69E-46 |
| MS.gene051760 | MS.gene51789 | 0.805696 | 1.18E-49 | -1.69E-46 |
| MS.gene051760 | MS.gene60935 | 0.819548 | 1.10E-52 | -1.69E-46 |
| MS.gene051760 | MS.gene82806 | 0.801 | 1.11E-48 | -1.69E-46 |
| MS.gene051760 | MS.gene84454 | 0.810473 | 1.13E-50 | -1.69E-46 |
| MS.gene051760 | MS.gene86844 | 0.803174 | 3.95E-49 | -1.69E-46 |
| MS.gene051760 | MS.gene87360 | 0.810248 | 1.27E-50 | -1.69E-46 |
| MS.gene051760 | MS.gene90370 | 0.803195 | 3.91E-49 | -1.69E-46 |
| MS.gene051760 | MS.gene90778 | 0.803725 | 3.04E-49 | -1.69E-46 |
| MS.gene051760 | MS.gene95698 | 0.819348 | 1.22E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051760.t1 | MTR_7g104800 | 97.500 | 80 | 2 | 0 | 1 | 80 | 133 | 212 | 1.45e-48 | 159 |
| MS.gene051760.t1 | MTR_4g052940 | 67.470 | 83 | 24 | 1 | 1 | 80 | 142 | 224 | 1.09e-33 | 118 |
| MS.gene051760.t1 | MTR_4g052940 | 67.470 | 83 | 24 | 1 | 1 | 80 | 142 | 224 | 5.34e-33 | 118 |
| MS.gene051760.t1 | MTR_7g010360 | 62.195 | 82 | 28 | 1 | 2 | 80 | 383 | 464 | 1.70e-28 | 106 |
| MS.gene051760.t1 | MTR_7g118120 | 56.579 | 76 | 33 | 0 | 5 | 80 | 143 | 218 | 1.51e-25 | 97.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051760.t1 | AT4G00830 | 59.211 | 76 | 31 | 0 | 5 | 80 | 155 | 230 | 1.12e-27 | 103 |
| MS.gene051760.t1 | AT4G00830 | 59.211 | 76 | 31 | 0 | 5 | 80 | 155 | 230 | 1.12e-27 | 103 |
| MS.gene051760.t1 | AT4G00830 | 59.211 | 76 | 31 | 0 | 5 | 80 | 155 | 230 | 1.12e-27 | 103 |
| MS.gene051760.t1 | AT3G52660 | 56.579 | 76 | 33 | 0 | 5 | 80 | 131 | 206 | 2.01e-27 | 103 |
| MS.gene051760.t1 | AT3G52660 | 56.579 | 76 | 33 | 0 | 5 | 80 | 131 | 206 | 2.01e-27 | 103 |
| MS.gene051760.t1 | AT3G52660 | 56.579 | 76 | 33 | 0 | 5 | 80 | 131 | 206 | 2.01e-27 | 103 |
| MS.gene051760.t1 | AT4G00830 | 60.294 | 68 | 27 | 0 | 5 | 72 | 155 | 222 | 4.90e-25 | 96.7 |
| MS.gene051760.t1 | AT4G00830 | 60.294 | 68 | 27 | 0 | 5 | 72 | 155 | 222 | 4.90e-25 | 96.7 |
| MS.gene051760.t1 | AT4G00830 | 51.316 | 76 | 37 | 0 | 5 | 80 | 155 | 230 | 7.82e-22 | 87.8 |
Find 23 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAAAGGAATTAGCATCTA+AGG | 0.262798 | 7.1:+6962030 | MS.gene051760:CDS |
| TGCTAATTCCTTTGTCTTGA+AGG | 0.283256 | 7.1:-6962023 | None:intergenic |
| TCTTCTACAGTCCACTCTTT+AGG | 0.290635 | 7.1:-6962338 | None:intergenic |
| GTTAAGCATCGGTTATTCAT+TGG | 0.392291 | 7.1:+6962308 | MS.gene051760:CDS |
| GGTTGGCCCTGGAGTCATTT+CGG | 0.392811 | 7.1:+6962382 | MS.gene051760:CDS |
| CATTTCGGTGGAACTGTTGA+AGG | 0.404786 | 7.1:+6962397 | MS.gene051760:CDS |
| GTTGAACAACTCTGAATTCA+AGG | 0.433692 | 7.1:+6962063 | MS.gene051760:CDS |
| GTTGTTGCCAAGGTTGGCCC+TGG | 0.464947 | 7.1:+6962371 | MS.gene051760:CDS |
| GGTCACAAAAGCATAACCTT+TGG | 0.479540 | 7.1:-6962002 | None:intergenic |
| AAGAAGGTTGTTGCCAAGGT+TGG | 0.496072 | 7.1:+6962365 | MS.gene051760:CDS |
| AGTTCCACCGAAATGACTCC+AGG | 0.523128 | 7.1:-6962389 | None:intergenic |
| AATGACTCCAGGGCCAACCT+TGG | 0.541179 | 7.1:-6962378 | None:intergenic |
| TATGAAGAAGGTTGTTGCCA+AGG | 0.545814 | 7.1:+6962361 | MS.gene051760:CDS |
| GACTGTAGAAGATATGAAGA+AGG | 0.552294 | 7.1:+6962349 | MS.gene051760:CDS |
| GCATAGGTAAGGGTTATGAA+GGG | 0.554101 | 7.1:+6961971 | None:intergenic |
| TTGGTAATGTTCCTAAAGAG+TGG | 0.554375 | 7.1:+6962327 | MS.gene051760:CDS |
| AACCGATGCTTAACTTGAGA+TGG | 0.558623 | 7.1:-6962299 | None:intergenic |
| CACCATCTCAAGTTAAGCAT+CGG | 0.570455 | 7.1:+6962297 | MS.gene051760:CDS |
| CATAGGTAAGGGTTATGAAG+GGG | 0.579849 | 7.1:+6961972 | None:intergenic |
| TGGCCCTGGAGTCATTTCGG+TGG | 0.622046 | 7.1:+6962385 | MS.gene051760:CDS |
| ATTAGCATCTAAGGCTCTCG+AGG | 0.629832 | 7.1:+6962039 | MS.gene051760:CDS |
| GTTCCACCGAAATGACTCCA+GGG | 0.672546 | 7.1:-6962388 | None:intergenic |
| ATGAAGGGGAAAGAAGCCAA+AGG | 0.709242 | 7.1:+6961986 | MS.gene051760:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATTTTATCCATTAAGC+TGG | + | chr7.1:6962204-6962223 | MS.gene051760:intron | 20.0% |
| !!! | TTGTCATTTTCTGTAATATT+TGG | + | chr7.1:6962106-6962125 | MS.gene051760:intron | 20.0% |
| ! | AACTGATTATTAACAAGTCA+TGG | + | chr7.1:6962142-6962161 | MS.gene051760:intron | 25.0% |
| !! | TCATTTTCTGTAATATTTGG+TGG | + | chr7.1:6962109-6962128 | MS.gene051760:intron | 25.0% |
| !! | ACTCTGAATTCAAGGTATTT+TGG | + | chr7.1:6962071-6962090 | MS.gene051760:intron | 30.0% |
| GACAAAGGAATTAGCATCTA+AGG | + | chr7.1:6962030-6962049 | MS.gene051760:CDS | 35.0% | |
| GACTGTAGAAGATATGAAGA+AGG | + | chr7.1:6962349-6962368 | MS.gene051760:CDS | 35.0% | |
| GTTGAACAACTCTGAATTCA+AGG | + | chr7.1:6962063-6962082 | MS.gene051760:CDS | 35.0% | |
| TGCTAATTCCTTTGTCTTGA+AGG | - | chr7.1:6962026-6962045 | None:intergenic | 35.0% | |
| ! | TTTTGTGACCTTCAAGACAA+AGG | + | chr7.1:6962015-6962034 | MS.gene051760:CDS | 35.0% |
| !! | GTTAAGCATCGGTTATTCAT+TGG | + | chr7.1:6962308-6962327 | MS.gene051760:CDS | 35.0% |
| !! | TTGGTAATGTTCCTAAAGAG+TGG | + | chr7.1:6962327-6962346 | MS.gene051760:CDS | 35.0% |
| !!! | AGCACTTTATTTTTCTTCCC+TGG | - | chr7.1:6962277-6962296 | None:intergenic | 35.0% |
| CACCATCTCAAGTTAAGCAT+CGG | + | chr7.1:6962297-6962316 | MS.gene051760:CDS | 40.0% | |
| TATGAAGAAGGTTGTTGCCA+AGG | + | chr7.1:6962361-6962380 | MS.gene051760:CDS | 40.0% | |
| TCTTCTACAGTCCACTCTTT+AGG | - | chr7.1:6962341-6962360 | None:intergenic | 40.0% | |
| ! | AACCGATGCTTAACTTGAGA+TGG | - | chr7.1:6962302-6962321 | None:intergenic | 40.0% |
| ! | GGTCACAAAAGCATAACCTT+TGG | - | chr7.1:6962005-6962024 | None:intergenic | 40.0% |
| AAGAAGGTTGTTGCCAAGGT+TGG | + | chr7.1:6962365-6962384 | MS.gene051760:CDS | 45.0% | |
| ACTACATGCAGCCAGCTTAA+TGG | - | chr7.1:6962218-6962237 | None:intergenic | 45.0% | |
| ATGAAGGGGAAAGAAGCCAA+AGG | + | chr7.1:6961986-6962005 | MS.gene051760:CDS | 45.0% | |
| ! | ATTAGCATCTAAGGCTCTCG+AGG | + | chr7.1:6962039-6962058 | MS.gene051760:CDS | 45.0% |
| AGTTCCACCGAAATGACTCC+AGG | - | chr7.1:6962392-6962411 | None:intergenic | 50.0% | |
| CACTCCTTTCTGCGTTTCCA+GGG | + | chr7.1:6962257-6962276 | MS.gene051760:intron | 50.0% | |
| GTTCCACCGAAATGACTCCA+GGG | - | chr7.1:6962391-6962410 | None:intergenic | 50.0% | |
| TCACTCCTTTCTGCGTTTCC+AGG | + | chr7.1:6962256-6962275 | MS.gene051760:intron | 50.0% | |
| TCTTCCCTGGAAACGCAGAA+AGG | - | chr7.1:6962264-6962283 | None:intergenic | 50.0% | |
| AATGACTCCAGGGCCAACCT+TGG | - | chr7.1:6962381-6962400 | None:intergenic | 55.0% | |
| ! | GGTTGGCCCTGGAGTCATTT+CGG | + | chr7.1:6962382-6962401 | MS.gene051760:CDS | 55.0% |
| TGGCCCTGGAGTCATTTCGG+TGG | + | chr7.1:6962385-6962404 | MS.gene051760:CDS | 60.0% | |
| ! | GTTGTTGCCAAGGTTGGCCC+TGG | + | chr7.1:6962371-6962390 | MS.gene051760:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 6961986 | 6962418 | 6961986 | ID=MS.gene051760 |
| chr7.1 | mRNA | 6961986 | 6962418 | 6961986 | ID=MS.gene051760.t1;Parent=MS.gene051760 |
| chr7.1 | exon | 6961986 | 6962084 | 6961986 | ID=MS.gene051760.t1.exon1;Parent=MS.gene051760.t1 |
| chr7.1 | CDS | 6961986 | 6962084 | 6961986 | ID=cds.MS.gene051760.t1;Parent=MS.gene051760.t1 |
| chr7.1 | exon | 6962278 | 6962418 | 6962278 | ID=MS.gene051760.t1.exon2;Parent=MS.gene051760.t1 |
| chr7.1 | CDS | 6962278 | 6962418 | 6962278 | ID=cds.MS.gene051760.t1;Parent=MS.gene051760.t1 |
| Gene Sequence |
| Protein sequence |
