Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055467.t1 | XP_027360988.1 | 92.3 | 104 | 8 | 0 | 9 | 112 | 2 | 105 | 1.60E-40 | 175.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055467.t1 | F4JJP1 | 70.9 | 103 | 30 | 0 | 10 | 112 | 147 | 249 | 4.5e-31 | 135.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055467.t1 | A0A445M7E6 | 89.4 | 104 | 11 | 0 | 9 | 112 | 2 | 105 | 7.7e-40 | 172.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene055467 | 0.809185 | 2.14E-50 | -1.69E-46 |
| MS.gene050551 | MS.gene055467 | 0.818678 | 1.73E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene055467 | 0.81666 | 4.93E-52 | -1.69E-46 |
| MS.gene051102 | MS.gene055467 | 0.817955 | 2.52E-52 | -1.69E-46 |
| MS.gene05157 | MS.gene055467 | 0.804693 | 1.91E-49 | -1.69E-46 |
| MS.gene051760 | MS.gene055467 | 0.805322 | 1.41E-49 | -1.69E-46 |
| MS.gene052397 | MS.gene055467 | 0.81541 | 9.38E-52 | -1.69E-46 |
| MS.gene052514 | MS.gene055467 | 0.828322 | 9.59E-55 | -1.69E-46 |
| MS.gene052516 | MS.gene055467 | 0.82493 | 6.18E-54 | -1.69E-46 |
| MS.gene052609 | MS.gene055467 | 0.81081 | 9.58E-51 | -1.69E-46 |
| MS.gene052665 | MS.gene055467 | 0.812264 | 4.63E-51 | -1.69E-46 |
| MS.gene052732 | MS.gene055467 | 0.820986 | 5.13E-53 | -1.69E-46 |
| MS.gene053143 | MS.gene055467 | 0.805594 | 1.24E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene055467 | 0.821408 | 4.10E-53 | -1.69E-46 |
| MS.gene053581 | MS.gene055467 | 0.820168 | 7.91E-53 | -1.69E-46 |
| MS.gene054584 | MS.gene055467 | 0.81964 | 1.04E-52 | -1.69E-46 |
| MS.gene054824 | MS.gene055467 | 0.824516 | 7.74E-54 | -1.69E-46 |
| MS.gene055465 | MS.gene055467 | 0.900048 | 1.08E-77 | -1.69E-46 |
| MS.gene055466 | MS.gene055467 | 0.854238 | 1.41E-61 | -1.69E-46 |
| MS.gene055467 | MS.gene055468 | 0.842682 | 2.23E-58 | -1.69E-46 |
| MS.gene055467 | MS.gene055469 | 0.890591 | 8.58E-74 | -1.69E-46 |
| MS.gene055467 | MS.gene055537 | 0.820229 | 7.66E-53 | -1.69E-46 |
| MS.gene055467 | MS.gene056005 | 0.803951 | 2.73E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene056308 | 0.826551 | 2.55E-54 | -1.69E-46 |
| MS.gene055467 | MS.gene056507 | 0.84014 | 1.04E-57 | -1.69E-46 |
| MS.gene055467 | MS.gene057446 | 0.807212 | 5.64E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene057915 | 0.802474 | 5.52E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene058087 | 0.851925 | 6.46E-61 | -1.69E-46 |
| MS.gene055467 | MS.gene058647 | 0.805888 | 1.07E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene058649 | 0.815588 | 8.56E-52 | -1.69E-46 |
| MS.gene055467 | MS.gene061006 | 0.840924 | 6.49E-58 | -1.69E-46 |
| MS.gene055467 | MS.gene06133 | 0.825554 | 4.40E-54 | -1.69E-46 |
| MS.gene055467 | MS.gene06258 | 0.820424 | 6.91E-53 | -1.69E-46 |
| MS.gene055467 | MS.gene064521 | 0.800291 | 1.54E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene065593 | 0.800411 | 1.46E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene066112 | 0.80346 | 3.45E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene067071 | 0.806713 | 7.20E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene068022 | 0.812815 | 3.51E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene068925 | 0.809486 | 1.85E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene06940 | 0.808057 | 3.73E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene069884 | 0.810924 | 9.05E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene070339 | 0.818476 | 1.92E-52 | -1.69E-46 |
| MS.gene055467 | MS.gene074435 | 0.800022 | 1.75E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene074519 | 0.822526 | 2.26E-53 | -1.69E-46 |
| MS.gene055467 | MS.gene074590 | 0.822528 | 2.26E-53 | -1.69E-46 |
| MS.gene055467 | MS.gene09087 | 0.811599 | 6.46E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene09947 | 0.814125 | 1.81E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene21144 | 0.809143 | 2.19E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene21960 | 0.814364 | 1.60E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene22309 | 0.825197 | 5.34E-54 | -1.69E-46 |
| MS.gene055467 | MS.gene22897 | 0.824094 | 9.72E-54 | -1.69E-46 |
| MS.gene055467 | MS.gene23105 | 0.812456 | 4.20E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene24268 | 0.800507 | 1.40E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene25810 | 0.81603 | 6.82E-52 | -1.69E-46 |
| MS.gene055467 | MS.gene26380 | 0.811631 | 6.36E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene26659 | 0.816676 | 4.89E-52 | -1.69E-46 |
| MS.gene055467 | MS.gene26851 | 0.800965 | 1.13E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene27302 | 0.809793 | 1.59E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene27568 | 0.813308 | 2.73E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene27700 | 0.823464 | 1.37E-53 | -1.69E-46 |
| MS.gene055467 | MS.gene29022 | 0.811457 | 6.94E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene30419 | 0.810046 | 1.40E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene30739 | 0.801877 | 7.32E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene31203 | 0.800681 | 1.29E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene31204 | 0.800959 | 1.13E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene31667 | 0.812981 | 3.22E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene31668 | 0.83641 | 9.51E-57 | -1.69E-46 |
| MS.gene055467 | MS.gene32028 | 0.825862 | 3.72E-54 | -1.69E-46 |
| MS.gene055467 | MS.gene34180 | 0.802396 | 5.72E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene34286 | 0.835036 | 2.12E-56 | -1.69E-46 |
| MS.gene055467 | MS.gene35631 | 0.818294 | 2.11E-52 | -1.69E-46 |
| MS.gene055467 | MS.gene36567 | 0.813052 | 3.11E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene38303 | 0.834731 | 2.53E-56 | -1.69E-46 |
| MS.gene055467 | MS.gene38561 | 0.80071 | 1.27E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene40739 | 0.82312 | 1.64E-53 | -1.69E-46 |
| MS.gene055467 | MS.gene40871 | 0.806163 | 9.40E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene41427 | 0.809649 | 1.70E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene41651 | 0.809683 | 1.68E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene42157 | 0.811572 | 6.55E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene44427 | 0.804307 | 2.30E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene44726 | 0.806853 | 6.72E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene46909 | 0.800808 | 1.21E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene48814 | 0.801838 | 7.45E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene49559 | 0.800815 | 1.21E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene49883 | 0.844868 | 5.79E-59 | -1.69E-46 |
| MS.gene055467 | MS.gene50163 | 0.816032 | 6.82E-52 | -1.69E-46 |
| MS.gene055467 | MS.gene50646 | 0.800282 | 1.55E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene52215 | 0.823632 | 1.25E-53 | -1.69E-46 |
| MS.gene055467 | MS.gene53064 | 0.80115 | 1.03E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene54530 | 0.809193 | 2.14E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene56717 | 0.812808 | 3.52E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene56748 | 0.801279 | 9.71E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene57213 | 0.81783 | 2.69E-52 | -1.69E-46 |
| MS.gene055467 | MS.gene59294 | 0.814888 | 1.22E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene59388 | 0.801026 | 1.09E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene61226 | 0.8096 | 1.75E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene62214 | 0.811389 | 7.17E-51 | -1.69E-46 |
| MS.gene055467 | MS.gene62682 | 0.817352 | 3.45E-52 | -1.69E-46 |
| MS.gene055467 | MS.gene63327 | 0.803695 | 3.08E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene63434 | 0.81738 | 3.40E-52 | -1.69E-46 |
| MS.gene055467 | MS.gene64671 | 0.824571 | 7.51E-54 | -1.69E-46 |
| MS.gene055467 | MS.gene68106 | 0.80672 | 7.17E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene68107 | 0.804048 | 2.60E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene68418 | 0.833377 | 5.52E-56 | -1.69E-46 |
| MS.gene055467 | MS.gene70730 | 0.825588 | 4.32E-54 | -1.69E-46 |
| MS.gene055467 | MS.gene71704 | 0.800977 | 1.12E-48 | -1.69E-46 |
| MS.gene055467 | MS.gene72011 | 0.806139 | 9.51E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene72555 | 0.821848 | 3.25E-53 | -1.69E-46 |
| MS.gene055467 | MS.gene73327 | 0.824354 | 8.45E-54 | -1.69E-46 |
| MS.gene055467 | MS.gene75604 | 0.802512 | 5.42E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene76549 | 0.80571 | 1.17E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene76734 | 0.807242 | 5.56E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene78189 | 0.828044 | 1.12E-54 | -1.69E-46 |
| MS.gene055467 | MS.gene82806 | 0.803162 | 3.98E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene82989 | 0.824045 | 9.99E-54 | -1.69E-46 |
| MS.gene055467 | MS.gene83055 | 0.828876 | 7.04E-55 | -1.69E-46 |
| MS.gene055467 | MS.gene84454 | 0.810666 | 1.03E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene84840 | 0.81924 | 1.29E-52 | -1.69E-46 |
| MS.gene055467 | MS.gene86844 | 0.803924 | 2.76E-49 | -1.69E-46 |
| MS.gene055467 | MS.gene88304 | 0.806834 | 6.78E-50 | -1.69E-46 |
| MS.gene055467 | MS.gene90610 | 0.818691 | 1.72E-52 | -1.69E-46 |
| MS.gene055467 | MS.gene95401 | 0.830511 | 2.82E-55 | -1.69E-46 |
| MS.gene055467 | MS.gene95698 | 0.824863 | 6.41E-54 | -1.69E-46 |
| MS.gene055467 | MS.gene97761 | 0.828758 | 7.52E-55 | -1.69E-46 |
| MS.gene055467 | MS.gene98395 | 0.800956 | 1.13E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055467.t1 | MTR_5g007350 | 95.238 | 105 | 5 | 0 | 8 | 112 | 164 | 268 | 4.87e-61 | 200 |
| MS.gene055467.t1 | MTR_4g478190 | 75.000 | 104 | 26 | 0 | 9 | 112 | 139 | 242 | 2.54e-47 | 161 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055467.t1 | AT1G65010 | 68.807 | 109 | 34 | 0 | 4 | 112 | 129 | 237 | 1.61e-41 | 145 |
| MS.gene055467.t1 | AT4G27595 | 70.874 | 103 | 30 | 0 | 10 | 112 | 134 | 236 | 1.05e-40 | 143 |
| MS.gene055467.t1 | AT4G27595 | 70.874 | 103 | 30 | 0 | 10 | 112 | 125 | 227 | 1.08e-40 | 143 |
| MS.gene055467.t1 | AT5G16730 | 57.282 | 103 | 41 | 1 | 10 | 112 | 148 | 247 | 1.81e-24 | 96.7 |
| MS.gene055467.t1 | AT3G02930 | 57.282 | 103 | 41 | 1 | 10 | 112 | 135 | 234 | 2.41e-22 | 90.9 |
| MS.gene055467.t1 | AT3G02930 | 57.282 | 103 | 41 | 1 | 10 | 112 | 137 | 236 | 2.53e-22 | 90.9 |
| MS.gene055467.t1 | AT3G02930 | 57.282 | 103 | 41 | 1 | 10 | 112 | 137 | 236 | 2.53e-22 | 90.9 |
Find 25 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAAGCAAATGAGAAACTT+AGG | 0.390966 | 5.1:+1838366 | None:intergenic |
| AGAGAAAGCGGAGATTCTTT+CGG | 0.435111 | 5.1:+1838674 | MS.gene055467:CDS |
| CCGAGCTGTTGAGCTGGAAC+AGG | 0.436065 | 5.1:+1838443 | MS.gene055467:CDS |
| CGGCTGAACTGATACGTTGA+AGG | 0.440735 | 5.1:+1838694 | MS.gene055467:CDS |
| TGTGAGGAACCAGCATGCTT+TGG | 0.468644 | 5.1:+1838521 | MS.gene055467:CDS |
| CGTCGGCATGACTCAGTGCC+TGG | 0.471915 | 5.1:-1838623 | None:intergenic |
| TGAGCTGGAACAGGCTGGAA+TGG | 0.495836 | 5.1:+1838452 | MS.gene055467:CDS |
| GAAGTTCCGAGCTGTTGAGC+TGG | 0.508758 | 5.1:+1838437 | MS.gene055467:CDS |
| GCTGTTGAGCTGGAACAGGC+TGG | 0.518387 | 5.1:+1838447 | MS.gene055467:CDS |
| TGAGAAACTTAGGGAGGCAA+TGG | 0.522492 | 5.1:+1838376 | MS.gene055467:CDS |
| GGAAGCAAATGAGAAACTTA+GGG | 0.544021 | 5.1:+1838367 | None:intergenic |
| TGCTCTTCTCGCCACCACTC+AGG | 0.550863 | 5.1:+1838551 | MS.gene055467:CDS |
| AATGGAAGCTGCTCAGAAGA+AGG | 0.554766 | 5.1:+1838470 | MS.gene055467:CDS |
| AGCTGCTCAGAAGAAGGAAG+AGG | 0.566414 | 5.1:+1838476 | MS.gene055467:CDS |
| GAGAAACTTAGGGAGGCAAT+GGG | 0.591625 | 5.1:+1838377 | MS.gene055467:CDS |
| CCTGTTCCAGCTCAACAGCT+CGG | 0.591772 | 5.1:-1838443 | None:intergenic |
| CAGACACATCCAAAGCATGC+TGG | 0.595358 | 5.1:-1838530 | None:intergenic |
| CTCAGAAGAAGGAAGAGGAA+TGG | 0.599765 | 5.1:+1838481 | MS.gene055467:CDS |
| GACTTGTGATGCAAAGAACC+AGG | 0.603818 | 5.1:+1838605 | MS.gene055467:CDS |
| AGAGAGAGCTTGAAAGTGTG+AGG | 0.609297 | 5.1:+1838505 | MS.gene055467:CDS |
| TGAGCTTCATGTAGAGAAAG+CGG | 0.632260 | 5.1:+1838662 | MS.gene055467:CDS |
| TCGTTGAAGCTCCTGAGTGG+TGG | 0.632686 | 5.1:-1838562 | None:intergenic |
| AAACTTAGGGAGGCAATGGG+TGG | 0.654540 | 5.1:+1838380 | MS.gene055467:CDS |
| AACTCGTTGAAGCTCCTGAG+TGG | 0.663561 | 5.1:-1838565 | None:intergenic |
| AGCAAATGAGAAACTTAGGG+AGG | 0.687762 | 5.1:+1838370 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGAGAAAGCGGAGATTCTTT+CGG | + | chr5.1:1838674-1838693 | MS.gene055467:CDS | 40.0% | |
| TGAGCTTCATGTAGAGAAAG+CGG | + | chr5.1:1838662-1838681 | MS.gene055467:CDS | 40.0% | |
| !!! | AGCAATTTTAGCTGCATCGT+CGG | - | chr5.1:1838643-1838662 | None:intergenic | 40.0% |
| AATGGAAGCTGCTCAGAAGA+AGG | + | chr5.1:1838470-1838489 | MS.gene055467:CDS | 45.0% | |
| CTCAGAAGAAGGAAGAGGAA+TGG | + | chr5.1:1838481-1838500 | MS.gene055467:CDS | 45.0% | |
| GACTTGTGATGCAAAGAACC+AGG | + | chr5.1:1838605-1838624 | MS.gene055467:CDS | 45.0% | |
| GAGAAACTTAGGGAGGCAAT+GGG | + | chr5.1:1838377-1838396 | MS.gene055467:CDS | 45.0% | |
| TGAGAAACTTAGGGAGGCAA+TGG | + | chr5.1:1838376-1838395 | MS.gene055467:CDS | 45.0% | |
| ! | AGAGAGAGCTTGAAAGTGTG+AGG | + | chr5.1:1838505-1838524 | MS.gene055467:CDS | 45.0% |
| AAACTTAGGGAGGCAATGGG+TGG | + | chr5.1:1838380-1838399 | MS.gene055467:CDS | 50.0% | |
| AACTCGTTGAAGCTCCTGAG+TGG | - | chr5.1:1838568-1838587 | None:intergenic | 50.0% | |
| AGCTGCTCAGAAGAAGGAAG+AGG | + | chr5.1:1838476-1838495 | MS.gene055467:CDS | 50.0% | |
| CAGACACATCCAAAGCATGC+TGG | - | chr5.1:1838533-1838552 | None:intergenic | 50.0% | |
| TGTGAGGAACCAGCATGCTT+TGG | + | chr5.1:1838521-1838540 | MS.gene055467:CDS | 50.0% | |
| CCTGTTCCAGCTCAACAGCT+CGG | - | chr5.1:1838446-1838465 | None:intergenic | 55.0% | |
| GAAGTTCCGAGCTGTTGAGC+TGG | + | chr5.1:1838437-1838456 | MS.gene055467:CDS | 55.0% | |
| TCGTTGAAGCTCCTGAGTGG+TGG | - | chr5.1:1838565-1838584 | None:intergenic | 55.0% | |
| TGAGCTGGAACAGGCTGGAA+TGG | + | chr5.1:1838452-1838471 | MS.gene055467:CDS | 55.0% | |
| CCGAGCTGTTGAGCTGGAAC+AGG | + | chr5.1:1838443-1838462 | MS.gene055467:CDS | 60.0% | |
| GCTGTTGAGCTGGAACAGGC+TGG | + | chr5.1:1838447-1838466 | MS.gene055467:CDS | 60.0% | |
| TGCTCTTCTCGCCACCACTC+AGG | + | chr5.1:1838551-1838570 | MS.gene055467:CDS | 60.0% | |
| CGTCGGCATGACTCAGTGCC+TGG | - | chr5.1:1838626-1838645 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 1838375 | 1838713 | 1838375 | ID=MS.gene055467 |
| chr5.1 | mRNA | 1838375 | 1838713 | 1838375 | ID=MS.gene055467.t1;Parent=MS.gene055467 |
| chr5.1 | exon | 1838375 | 1838713 | 1838375 | ID=MS.gene055467.t1.exon1;Parent=MS.gene055467.t1 |
| chr5.1 | CDS | 1838375 | 1838713 | 1838375 | ID=cds.MS.gene055467.t1;Parent=MS.gene055467.t1 |
| Gene Sequence |
| Protein sequence |
