Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051851.t1 | XP_024638198.1 | 93.1 | 509 | 35 | 0 | 1 | 509 | 1 | 509 | 3.80E-270 | 940.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051851.t1 | Q5GKZ7 | 79.5 | 512 | 88 | 8 | 1 | 509 | 1 | 498 | 6.3e-229 | 794.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051851.t1 | A0A396INY4 | 93.1 | 509 | 35 | 0 | 1 | 509 | 1 | 509 | 2.8e-270 | 940.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051851 | MS.gene061836 | 0.810848 | 9.40E-51 | -1.69E-46 |
| MS.gene051851 | MS.gene061837 | 0.817957 | 2.52E-52 | -1.69E-46 |
| MS.gene051851 | MS.gene061838 | 0.826731 | 2.31E-54 | -1.69E-46 |
| MS.gene051851 | MS.gene06354 | 0.815764 | 7.82E-52 | -1.69E-46 |
| MS.gene051851 | MS.gene09895 | 0.824127 | 9.55E-54 | -1.69E-46 |
| MS.gene051851 | MS.gene57577 | 0.894415 | 2.52E-75 | -1.69E-46 |
| MS.gene051851 | MS.gene89532 | 0.825061 | 5.75E-54 | -1.69E-46 |
| MS.gene051851 | MS.gene94818 | 0.800696 | 1.28E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051851.t1 | MTR_4g125980 | 97.446 | 509 | 11 | 1 | 1 | 509 | 1 | 507 | 0.0 | 1022 |
| MS.gene051851.t1 | MTR_4g125980 | 97.380 | 458 | 10 | 1 | 1 | 458 | 1 | 456 | 0.0 | 917 |
| MS.gene051851.t1 | MTR_2g039940 | 74.464 | 513 | 105 | 6 | 21 | 509 | 29 | 539 | 0.0 | 788 |
| MS.gene051851.t1 | MTR_4g125980 | 98.925 | 372 | 4 | 0 | 138 | 509 | 1 | 372 | 0.0 | 765 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051851.t1 | AT2G19450 | 77.830 | 424 | 85 | 3 | 85 | 508 | 105 | 519 | 0.0 | 703 |
Find 108 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAAGCCTGGCCTACTTTA+TGG | 0.102319 | 8.2:-6574338 | MS.gene051851:CDS |
| TATAGAATGTTTCCCTTAAA+AGG | 0.165077 | 8.2:+6573107 | None:intergenic |
| GTTTAGTGGAATTATGTTTC+AGG | 0.291045 | 8.2:-6570306 | MS.gene051851:intron |
| ATAATATTTACTGGAGTTAT+GGG | 0.300470 | 8.2:-6573338 | MS.gene051851:CDS |
| TGAATCTAGGTTGAATATAT+TGG | 0.302386 | 8.2:-6572268 | MS.gene051851:intron |
| GTCAAGCTGATAATATTTAC+TGG | 0.305250 | 8.2:-6573347 | MS.gene051851:CDS |
| GATAATATTTACTGGAGTTA+TGG | 0.318190 | 8.2:-6573339 | MS.gene051851:CDS |
| AATTGGCAGCAGCGATGTTT+TGG | 0.324920 | 8.2:+6577896 | None:intergenic |
| GTTCAAAATCATTGAGAGAT+TGG | 0.327935 | 8.2:-6576555 | MS.gene051851:CDS |
| TCTACTGCTTCTTCCACCTT+TGG | 0.347716 | 8.2:-6573025 | MS.gene051851:intron |
| CGTTGAGAACCGTTGGGTTC+CGG | 0.353692 | 8.2:+6578055 | None:intergenic |
| CCCTTCCATTCGAAAGGGTT+GGG | 0.357388 | 8.2:-6573384 | MS.gene051851:CDS |
| TTGGCAGAGCTTCTTCGATT+TGG | 0.360389 | 8.2:-6572249 | MS.gene051851:CDS |
| GACCTGAGGATTACTAAAAT+AGG | 0.367816 | 8.2:+6576112 | None:intergenic |
| TGCTACTTGCTTGCATTGTA+TGG | 0.370577 | 8.2:-6574836 | MS.gene051851:CDS |
| GCATATTCCATGTGAGTATT+GGG | 0.384799 | 8.2:+6574376 | None:intergenic |
| TCCTTTGGCGGCCTTTATAG+TGG | 0.384946 | 8.2:-6576398 | MS.gene051851:CDS |
| GTATTGGAGGATGTGGAATA+TGG | 0.390765 | 8.2:-6572062 | MS.gene051851:intron |
| CAGAGCCACACATATAGATT+TGG | 0.393170 | 8.2:+6573053 | None:intergenic |
| TAGTCTTGCAATATTTCCTT+TGG | 0.398091 | 8.2:-6576413 | MS.gene051851:CDS |
| GGTTGATTAAGACTGGCTTT+TGG | 0.406433 | 8.2:-6576582 | MS.gene051851:CDS |
| TTCAAGTTGTGGGCGTTTAG+TGG | 0.408945 | 8.2:-6570320 | MS.gene051851:CDS |
| TTGCATTGTATGGTTAAAAC+TGG | 0.410660 | 8.2:-6574826 | MS.gene051851:CDS |
| TGCATATTCCATGTGAGTAT+TGG | 0.413902 | 8.2:+6574375 | None:intergenic |
| CGAATCATCCATTTGTGAAC+AGG | 0.426743 | 8.2:+6571747 | None:intergenic |
| TTGCCACATATTCAAGTTGT+GGG | 0.429494 | 8.2:-6570330 | MS.gene051851:CDS |
| CCCCTTCCATTCGAAAGGGT+TGG | 0.437480 | 8.2:-6573385 | MS.gene051851:CDS |
| AATTTAATCGAAGAAATATC+AGG | 0.447275 | 8.2:+6577856 | None:intergenic |
| GAAGGGGTGCGAGGATATCT+TGG | 0.450792 | 8.2:+6573401 | None:intergenic |
| TCTTGCAATATTTCCTTTGG+CGG | 0.454969 | 8.2:-6576410 | MS.gene051851:CDS |
| TACGGTTGGTTGATTAAGAC+TGG | 0.460510 | 8.2:-6576589 | MS.gene051851:CDS |
| ACTTACAAATTCAATTGAAA+AGG | 0.469129 | 8.2:-6574769 | MS.gene051851:intron |
| CCGCTGACGGAGGCTGCGCT+CGG | 0.471595 | 8.2:+6578009 | None:intergenic |
| CTTGCCACATATTCAAGTTG+TGG | 0.472541 | 8.2:-6570331 | MS.gene051851:CDS |
| CAGCTTCAGAACTCTTTCAA+TGG | 0.473289 | 8.2:+6573082 | None:intergenic |
| TGTTTCCCTTAAAAGGATGC+TGG | 0.475871 | 8.2:+6573114 | None:intergenic |
| TCCTCAGACATGGAAAATAT+AGG | 0.477175 | 8.2:+6571721 | None:intergenic |
| GAATCGTCTGCCACGGTGGA+AGG | 0.484354 | 8.2:-6577979 | MS.gene051851:CDS |
| TATCATTGAGAATTTAATGA+AGG | 0.486505 | 8.2:-6577091 | MS.gene051851:intron |
| TGTTGCAGCCTGTTCACAAA+TGG | 0.490462 | 8.2:-6571755 | MS.gene051851:intron |
| CGCACCCCTTCCATTCGAAA+GGG | 0.495380 | 8.2:-6573389 | MS.gene051851:CDS |
| ACGGTGGAAGGCGAGAGTTC+CGG | 0.499518 | 8.2:-6577967 | MS.gene051851:CDS |
| AAATAAATTCAGCAACTCAA+TGG | 0.506072 | 8.2:-6569803 | MS.gene051851:intron |
| ACGACCCTCACGGTGCCGTC+CGG | 0.508155 | 8.2:-6578074 | MS.gene051851:CDS |
| GGCCTTTATAGTGGAAAAGT+TGG | 0.508985 | 8.2:-6576389 | MS.gene051851:CDS |
| AACCTGACGGAAAATGTTAT+CGG | 0.510027 | 8.2:+6577777 | None:intergenic |
| ATCCTTCACCGACTCTTCAC+CGG | 0.518020 | 8.2:+6577948 | None:intergenic |
| TTATGTAAGCTTCAAAAGCC+TGG | 0.519195 | 8.2:-6574350 | MS.gene051851:CDS |
| GAGGAGTCGTTGAGAACCGT+TGG | 0.519542 | 8.2:+6578048 | None:intergenic |
| TTACGATGAGCGGGAACAGA+AGG | 0.520023 | 8.2:+6577823 | None:intergenic |
| GCCATCAGTATTGGAGGATG+TGG | 0.524661 | 8.2:-6572069 | MS.gene051851:intron |
| TGCTCCTACGTTATGTTACC+AGG | 0.526894 | 8.2:-6574314 | MS.gene051851:intron |
| ACCCAACCCTTTCGAATGGA+AGG | 0.531700 | 8.2:+6573383 | None:intergenic |
| AATCAGATGCAGAGTCATGC+TGG | 0.534169 | 8.2:-6577162 | MS.gene051851:intron |
| GAGCAACCATAAAGTAGGCC+AGG | 0.534701 | 8.2:+6574332 | None:intergenic |
| ACTGCTGCCATCAGTATTGG+AGG | 0.536832 | 8.2:-6572075 | MS.gene051851:intron |
| TCGCACCCCTTCCATTCGAA+AGG | 0.538191 | 8.2:-6573390 | MS.gene051851:CDS |
| ACGCCCACAACTTGAATATG+TGG | 0.541477 | 8.2:+6570327 | None:intergenic |
| GACGGCACCGTGAGGGTCGT+CGG | 0.542876 | 8.2:+6578077 | None:intergenic |
| GTTTCCCTTAAAAGGATGCT+GGG | 0.543462 | 8.2:+6573115 | None:intergenic |
| TCCCGCTCATCGTAAAATCA+AGG | 0.547682 | 8.2:-6577815 | MS.gene051851:CDS |
| CGTAGGAGCAACCATAAAGT+AGG | 0.548280 | 8.2:+6574327 | None:intergenic |
| CTTCTTCGAATCGTCTGCCA+CGG | 0.559270 | 8.2:-6577986 | MS.gene051851:CDS |
| GACATTACCAACACATGAAG+AGG | 0.559412 | 8.2:+6576530 | None:intergenic |
| ATTGGCCCCTCTTCATGTGT+TGG | 0.564337 | 8.2:-6576537 | MS.gene051851:intron |
| GTCAGTGCCGATAGAGTCAT+CGG | 0.569995 | 8.2:+6577918 | None:intergenic |
| TCCACTATAAAGGCCGCCAA+AGG | 0.571464 | 8.2:+6576397 | None:intergenic |
| CCGAGCGCAGCCTCCGTCAG+CGG | 0.572246 | 8.2:-6578009 | MS.gene051851:CDS |
| AGGAGTCGTTGAGAACCGTT+GGG | 0.575988 | 8.2:+6578049 | None:intergenic |
| TTCCGGTGAAGAGTCGGTGA+AGG | 0.582558 | 8.2:-6577950 | MS.gene051851:CDS |
| TCTAGTTCCGATGACTCTAT+CGG | 0.584171 | 8.2:-6577925 | MS.gene051851:CDS |
| CCCAACCCTTTCGAATGGAA+GGG | 0.586326 | 8.2:+6573384 | None:intergenic |
| AAGAGATGGAATACCAAAGG+TGG | 0.589844 | 8.2:+6573012 | None:intergenic |
| CTACTATACAGAGGTTGAAG+AGG | 0.600310 | 8.2:+6577139 | None:intergenic |
| CTGTTCCAAATCTATATGTG+TGG | 0.600615 | 8.2:-6573058 | MS.gene051851:CDS |
| CGAGAGTTCCGGTGAAGAGT+CGG | 0.602185 | 8.2:-6577956 | MS.gene051851:CDS |
| ACATTACCAACACATGAAGA+GGG | 0.608800 | 8.2:+6576531 | None:intergenic |
| TAACACCCAACCCTTTCGAA+TGG | 0.610126 | 8.2:+6573379 | None:intergenic |
| GGTTCCGGACGGCACCGTGA+GGG | 0.610138 | 8.2:+6578070 | None:intergenic |
| CCAACCCTTTCGAATGGAAG+GGG | 0.615118 | 8.2:+6573385 | None:intergenic |
| TTCGAAGAAGCCGCTGACGG+AGG | 0.616528 | 8.2:+6577999 | None:intergenic |
| GAATGCAAGAACTGTTGAAG+AGG | 0.622975 | 8.2:-6572199 | MS.gene051851:intron |
| CGGTGCCGTCCGGAACCCAA+CGG | 0.624501 | 8.2:-6578064 | MS.gene051851:CDS |
| GGGTTCCGGACGGCACCGTG+AGG | 0.624853 | 8.2:+6578069 | None:intergenic |
| GGTATACCAGTCCTCAGACA+TGG | 0.627497 | 8.2:+6571711 | None:intergenic |
| GGGTCGTCGGAAATCGCCAT+CGG | 0.633004 | 8.2:+6578090 | None:intergenic |
| GGTTTCTGCTTTGTTCCATG+AGG | 0.636622 | 8.2:-6570632 | MS.gene051851:intron |
| GCGATTTCCGACGACCCTCA+CGG | 0.636953 | 8.2:-6578084 | MS.gene051851:CDS |
| CACAACTTGAATATGTGGCA+AGG | 0.637365 | 8.2:+6570332 | None:intergenic |
| GAGAACCGTTGGGTTCCGGA+CGG | 0.638001 | 8.2:+6578059 | None:intergenic |
| GTCTGCTGTTCACTGCAACA+AGG | 0.639453 | 8.2:+6577115 | None:intergenic |
| TTTCGAATGGAAGGGGTGCG+AGG | 0.641294 | 8.2:+6573392 | None:intergenic |
| GAACAAAGCAGAAACCATGA+AGG | 0.644488 | 8.2:+6570639 | None:intergenic |
| TAATTAGTTATCAAGACCAA+AGG | 0.651239 | 8.2:+6569832 | None:intergenic |
| TCTGAGGACTGGTATACCAA+AGG | 0.652186 | 8.2:-6571706 | MS.gene051851:intron |
| CTTCGAATCGTCTGCCACGG+TGG | 0.653441 | 8.2:-6577983 | MS.gene051851:CDS |
| CGATTCGAAGAAGCCGCTGA+CGG | 0.657388 | 8.2:+6577996 | None:intergenic |
| TATAATCATTGCAACAACTG+CGG | 0.672463 | 8.2:-6576143 | MS.gene051851:CDS |
| AGCGCTTCCCAATACTCACA+TGG | 0.675287 | 8.2:-6574383 | MS.gene051851:CDS |
| TAGTATAGTAGTACACACAT+AGG | 0.676722 | 8.2:+6569622 | None:intergenic |
| CTGAGTGCAGCTGTGCATCG+CGG | 0.700113 | 8.2:-6570357 | MS.gene051851:intron |
| GATGAGCGGGAACAGAAGGA+CGG | 0.707063 | 8.2:+6577827 | None:intergenic |
| GCTACCTGGTAACATAACGT+AGG | 0.709981 | 8.2:+6574310 | None:intergenic |
| CAACAAGGACTACTATACAG+AGG | 0.712127 | 8.2:+6577130 | None:intergenic |
| CATTACCAACACATGAAGAG+GGG | 0.715726 | 8.2:+6576532 | None:intergenic |
| CGGTCTGCGACGTAAAACAG+AGG | 0.728316 | 8.2:+6578029 | None:intergenic |
| GGAACTCTCGCCTTCCACCG+TGG | 0.744840 | 8.2:+6577969 | None:intergenic |
| AAAATGTTATCGGAGCTGAG+TGG | 0.746573 | 8.2:+6577787 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 6569585 | 6578109 | 6569585 | ID=MS.gene051851 |
| chr8.2 | mRNA | 6569585 | 6578109 | 6569585 | ID=MS.gene051851.t1;Parent=MS.gene051851 |
| chr8.2 | exon | 6577780 | 6578109 | 6577780 | ID=MS.gene051851.t1.exon1;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6577780 | 6578109 | 6577780 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6577092 | 6577178 | 6577092 | ID=MS.gene051851.t1.exon2;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6577092 | 6577178 | 6577092 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6576538 | 6576611 | 6576538 | ID=MS.gene051851.t1.exon3;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6576538 | 6576611 | 6576538 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6576363 | 6576438 | 6576363 | ID=MS.gene051851.t1.exon4;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6576363 | 6576438 | 6576363 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6576115 | 6576182 | 6576115 | ID=MS.gene051851.t1.exon5;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6576115 | 6576182 | 6576115 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6574770 | 6574893 | 6574770 | ID=MS.gene051851.t1.exon6;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6574770 | 6574893 | 6574770 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6574315 | 6574410 | 6574315 | ID=MS.gene051851.t1.exon7;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6574315 | 6574410 | 6574315 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6573322 | 6573423 | 6573322 | ID=MS.gene051851.t1.exon8;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6573322 | 6573423 | 6573322 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6573026 | 6573162 | 6573026 | ID=MS.gene051851.t1.exon9;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6573026 | 6573162 | 6573026 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6572200 | 6572281 | 6572200 | ID=MS.gene051851.t1.exon10;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6572200 | 6572281 | 6572200 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6572063 | 6572083 | 6572063 | ID=MS.gene051851.t1.exon11;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6572063 | 6572083 | 6572063 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6571707 | 6571769 | 6571707 | ID=MS.gene051851.t1.exon12;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6571707 | 6571769 | 6571707 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6570633 | 6570680 | 6570633 | ID=MS.gene051851.t1.exon13;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6570633 | 6570680 | 6570633 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6570307 | 6570369 | 6570307 | ID=MS.gene051851.t1.exon14;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6570307 | 6570369 | 6570307 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6569804 | 6569857 | 6569804 | ID=MS.gene051851.t1.exon15;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6569804 | 6569857 | 6569804 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| chr8.2 | exon | 6569585 | 6569689 | 6569585 | ID=MS.gene051851.t1.exon16;Parent=MS.gene051851.t1 |
| chr8.2 | CDS | 6569585 | 6569689 | 6569585 | ID=cds.MS.gene051851.t1;Parent=MS.gene051851.t1 |
| Gene Sequence |
| Protein sequence |
